- Inhibition of KIF20A suppresses the replication of influenza A virus by inhibiting viral entry
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Hoyeon Jeon , Younghyun Lim , In-Gu Lee , Dong-In Kim , Keun Pil Kim , So-Hee Hong , Jeongkyu Kim , Youn-Sang Jung , Young-Jin Seo
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J. Microbiol. 2022;60(11):1113-1121. Published online November 1, 2022
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DOI: https://doi.org/10.1007/s12275-022-2436-x
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 Abstract
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The influenza A virus (IAV) has caused several pandemics,
and therefore there are many ongoing efforts to identify novel
antiviral therapeutic strategies including vaccines and antiviral
drugs. However, influenza viruses continuously undergo
antigenic drift and shift, resulting in the emergence of mutated
viruses. In turn, this decreases the efficiency of existing vaccines
and antiviral drugs to control IAV infection. Therefore,
this study sought to identify alternative therapeutic strategies
targeting host cell factors rather than viruses to avoid infection
by mutated viruses. Particularly, we investigated the role
of KIF20A that is one of kinesin superfamily proteins in the
replication of IAV. The KIF20A increased viral protein levels in
IAV-infected cells by regulating the initial entry stage during
viral infection. Furthermore, the KIF20A inhibitor significantly
suppressed viral replication, which protected mice from morbidity
and mortality. Therefore, our findings demonstrated
that KIF20A is highly involved in the viral replication process
and viral propagation both in vitro and in vivo, and could thus
be used as a target for the development of novel antiviral drugs.
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Citations
Citations to this article as recorded by  - Zika virus entry inhibitors
Weiyi Yin, Rui Zhou, Wencui Zhang, Yanping Li
Bioorganic & Medicinal Chemistry.2026; 137: 118611. CrossRef - Inhibiting kinesin family member 20A disrupts Zika virus entry by blocking internalization
Jeonghyeon Lee, Younghyun Lim, Hyeong-Rae Kim, Yong-Bin Cho, In-Gu Lee, Young-Jin Seo
Journal of Microbiology.2025; 63(6): e2503008. CrossRef - Deciphering European Sea Bass (Dicentrarchus labrax) Resistance to Nervous Necrosis Virus by Transcriptome Analysis from Early Infection Towards Establishment of Virus Carrier State
Dimitra K. Toubanaki, Odysseas-Panagiotis Tzortzatos, Antonia Efstathiou, Vasileios Bakopoulos, Evdokia Karagouni
International Journal of Molecular Sciences.2025; 26(18): 9220. CrossRef - Emerging roles of cytoskeletal transport and scaffold systems in human viral propagation
Younghyun Lim, Yong-Bin Cho, Young-Jin Seo
Animal Cells and Systems.2024; 28(1): 506. CrossRef
- Yeast polyubiquitin unit regulates synaptonemal complex formation and recombination during meiosis
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Min-Kyung Jo , Kiwon Rhee , Keun Pil Kim , Soogil Hong
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J. Microbiol. 2022;60(7):705-714. Published online July 4, 2022
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DOI: https://doi.org/10.1007/s12275-022-2204-y
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651
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Abstract
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Ubiquitin is highly conserved in most eukaryotes and involved
in diverse physiological processes, including cell division, protein
quality control, and protein degradation mediated by the
ubiquitin-proteasome system after heat shock, glucose-starvation,
and oxidative stress. However, the role of the ubiquitin
gene UBI4, which contains five consecutive head-to-tail ubiquitin
repeats, in meiosis has not been investigated. In this
study, we show that the Saccharomyces cerevisiae polyubiquitin
precursor gene, UBI4, is required to promote synaptonemal
complex (SC) formation and suppress excess doublestrand
break formation. Moreover, the proportion of Zip1
polycomplexes, which indicate abnormal SC formation, in
cells with a mutation in UBI4 (i.e., ubi4Δ cells) is higher than
that of wild-type cells, implying that the UBI4 plays an important
role in the early meiotic prophase I. Interestingly, although
ubi4Δ cells rarely form full-length SCs in the pachytene
stage of prophase I, the Zip3 foci are still seen, as in
wild-type cells. Moreover, ubi4Δ cells proficiently form crossover
and noncrossover products with a slight delay compared
to wild-type cells, suggesting that UBI4 is dispensable in SCcoupled
recombination. Our findings demonstrate that UBI4
exhibits dual functions that are associated with both positive
and negative roles in SC formation and recombination during
meiosis.
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Citations
Citations to this article as recorded by -
The deubiquitinase Usp7 in
Drosophila melanogaster
is required for synaptonemal complex maintenance
Cathleen M. Lake, Jennifer Gardner, Salam Briggs, Zulin Yu, Grace McKown, R. Scott Hawley
Proceedings of the National Academy of Sciences.2024;[Epub] CrossRef
- Meiotic prophase roles of Pds5 in recombination and chromosome condensation in budding yeast
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Jeong Hwan Joo , Hyun Ah Kang , Keun Pil Kim , Soogil Hong
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J. Microbiol. 2022;60(2):177-186. Published online February 1, 2022
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DOI: https://doi.org/10.1007/s12275-022-1635-9
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658
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Genetic variation in eukaryotes is mediated during meiosis by
the exchange of genetic material between homologous chromosomes
to produce recombinant chromosomes. Cohesin is
essential to promote proper chromosome segregation, chromosome
morphogenesis, and recombination in meiotic cells.
Cohesin consists of three main subunits–Smc1, Smc3, and the
kleisin subunit Mcd1/Scc1 (Rec8 in meiosis)–and cohesin accessory
factors. In Saccharomyces cerevisiae, the cohesin regulatory
subunit Pds5 plays a role in homolog pairing, meiotic
axis formation, and interhomolog recombination. In this
study, we examine the prophase functions of Pds5 by performing
physical analysis of recombination and three-dimensional
high-resolution microscopy analysis to identify its roles in
meiosis-specific recombination and chromosome morphogenesis.
To investigate whether Pds5 plays a role in mitoticlike
recombination, we inhibited Mek1 kinase activity, which
result
ed in switching to sister template bias by Rad51-dependent
recombination. Reductions in double-strand breaks
and crossover products and defective interhomolog recombination
occurred in the absence of Pds5. Furthermore, recombination
intermediates, including single-end invasion
and double-Holliday junction, were reduced in the absence
of Pds5 with Mek1 kinase inactivation compared to Mek1
kinase inactivation cells. Interestingly, the absence of Pds5
result
ed in increasing numbers of chromosomes with hypercompaction
of the chromosome axis. Thus, we suggest that
Pds5 plays an essential role in recombination by suppressing
the pairing of sister chromatids and abnormal compaction
of the chromosome axis.
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Citations
Citations to this article as recorded by - Recombination-coupled DNA synthesis facilitates post-invasion steps in meiotic crossover and noncrossover formations
Hyungseok Choi, Jun Seo Lee, Jeong H Joo, Soogene Lee, Keun P Kim
Nucleic Acids Research.2025;[Epub] CrossRef - Multiple Autopolyploid Arabidopsis lyrata Populations Stabilized by Long-Range Adaptive Introgression Across Eurasia
Alison D Scott, Uliana K Kolesnikova, Anna Glushkevich, Laura Steinmann, Nikita P Tikhomirov, Ursula Pfordt, Magdalena Bohutínská, Robin Burns, Alexey P Seregin, Filip Kolar, Roswitha Schmickl, Polina Yu Novikova, Kathryn Hodgins
Molecular Biology and Evolution.2025;[Epub] CrossRef - RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination
Jeong H Joo, Soogil Hong, Mika T Higashide, Eui-Hwan Choi, Seobin Yoon, Min-Su Lee, Hyun Ah Kang, Akira Shinohara, Nancy Kleckner, Keun P Kim
Nucleic Acids Research.2024; 52(7): 3794. CrossRef - Cohesin is required for meiotic spindle assembly independent of its role in cohesion in C. elegans
Karen P. McNally, Elizabeth A. Beath, Brennan M. Danlasky, Consuelo Barroso, Ting Gong, Wenzhe Li, Enrique Martinez-Perez, Francis J. McNally, Sarit Smolikove
PLOS Genetics.2022; 18(10): e1010136. CrossRef - Yeast polyubiquitin unit regulates synaptonemal complex formation and recombination during meiosis
Min-Kyung Jo, Kiwon Rhee, Keun Pil Kim, Soogil Hong
Journal of Microbiology.2022; 60(7): 705. CrossRef
- Molecular characterization of Hsf1 as a master regulator of heat shock response in the thermotolerant methylotrophic yeast Ogataea parapolymorpha
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Jin Ho Choo , Su-Bin Lee , Hye Yun Moon , Kun Hwa Lee , Su Jin Yoo , Keun Pil Kim , Hyun Ah Kang
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J. Microbiol. 2021;59(2):151-163. Published online February 1, 2021
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DOI: https://doi.org/10.1007/s12275-021-0646-2
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595
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5
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6
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Abstract
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Ogataea parapolymorpha (Hansenula polymorpha DL-1) is
a thermotolerant methylotrophic yeast with biotechnological
applications. Here, O. parapolymorpha genes whose expression
is induced in response to heat shock were identified by
transcriptome analysis and shown to possess heat shock elements
(HSEs) in their promoters. The function of O. parapolymorpha
HSF1 encoding a putative heat shock transcription
factor 1 (OpHsf1) was characterized in the context of heat
stress response. Despite exhibiting low sequence identity
(26%) to its Saccharomyces cerevisiae homolog, OpHsf1 harbors
conserved domains including a DNA binding domain
(DBD), domains involved in trimerization (TRI), transcriptional
activation (AR1, AR2), transcriptional repression (CE2),
and a C-terminal modulator (CTM) domain. OpHSF1 could
complement the temperature sensitive (Ts) phenotype of a
S. cerevisiae hsf1 mutant. An O. parapolymorpha strain with
an H221R mutation in the DBD domain of OpHsf1 exhibited
significantly retarded growth and a Ts phenotype. Intriguingly,
the expression of heat-shock-protein‒coding genes harboring
HSEs was significantly decreased in the H221R mutant
strain, even under non-stress conditions, indicating the importance
of the DBD for the basal growth of O. parapolymorpha.
Notably, even though the deletion of C-terminal domains
(ΔCE2, ΔAR2, ΔCTM) of OpHsf1 destroyed complementation
of the growth defect of the S. cerevisiae hsf1 strain,
the C-terminal domains were shown to be dispensable in O.
parapolymorpha. Overexpression of OpHsf1 in S. cerevisiae
increased resistance to transient heat shock, supporting the
idea that OpHsf1 could be useful in the development of heatshock‒
resistant yeast host strains.
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Citations
Citations to this article as recorded by - Thermotolerant yeasts and their biotechnological applications
Roksolana Vasylyshyn, Justyna Ruchala, Kostyantyn Dmytruk, Andriy Sibirny
Trends in Biotechnology.2026;[Epub] CrossRef - Lignocellulosic biomass fermentation: a roadmap for Candida famata and Ogataea polymorpha
Dominik Wojdyła, Roksolana Vasylyshyn, Alicja Najdecka, Justyna Ruchala
FEMS Yeast Research.2025;[Epub] CrossRef - Sucrose‐Induced Transcriptomic Response in Ogataea polymorpha TBRC 4839 Reveals its Potential for Recombinant Protein Production
Somsak Likhitrattanapisal, Chitwadee Phithakrotchanakoon, Aekkachai Puseenam, Paopit Siriarchawatana, Natta Wiriyakun, Jiraprapa Nirapun, Warasirin Sornlek, Supawadee Ingsriswang, Niran Roongsawang
Yeast.2025; 42(8-10): 181. CrossRef - A comprehensive review and comparison of L-tryptophan biosynthesis in Saccharomyces cerevisiae and Escherichia coli
Xinru Ren, Yue Wei, Honglu Zhao, Juanjuan Shao, Fanli Zeng, Zhen Wang, Li Li
Frontiers in Bioengineering and Biotechnology.2023;[Epub] CrossRef - Heat shock in Cronobacter sakazakii induces direct protection and cross-protection against simulated gastric fluid stress
Hongmei Niu, MingzheYang, Yonghua Qi, Yangtai Liu, Xiang Wang, Qingli Dong
Food Microbiology.2022; 103: 103948. CrossRef - A review of yeast: High cell-density culture, molecular mechanisms of stress response and tolerance during fermentation
Dongxu Shen, Xiaoli He, Peifang Weng, Yanan Liu, Zufang Wu
FEMS Yeast Research.2022;[Epub] CrossRef
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