Journal of Microbiology

Volume 58(1); January 2020

Review

[Minireview]Recent advances in genetic engineering tools based on synthetic biology: Jun Ren , Jingyu Lee , Dokyun Na; J. Microbiol. 2020;58(1):1-10. Published online January 2, 2020; DOI: https://doi.org/10.1007/s12275-020-9334-x

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Genome-scale engineering is a crucial methodology to rationally regulate microbiological system operations, leading to expected biological behaviors or enhanced bioproduct yields. Over the past decade, innovative genome modification technologies have been developed for effectively regulating and manipulating genes at the genome level. Here, we discuss the current genome-scale engineering technologies used for microbial engineering. Recently developed strategies, such as clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9, multiplex automated genome engineering (MAGE), promoter engineering, CRISPR-based regulations, and synthetic small regulatory RNA (sRNA)-based knockdown, are considered as powerful tools for genome-scale engineering in microbiological systems. MAGE, which modifies specific nucleotides of the genome sequence, is utilized as a genome-editing tool. Contrastingly, synthetic sRNA, CRISPRi, and CRISPRa are mainly used to regulate gene expression without modifying the genome sequence. This review introduces the recent genome-scale editing and regulating technologies and their applications in metabolic engineering.

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Construction of a tunable promoter library to optimize gene expression in Methylomonas sp. DH-1, a methanotroph, and its application to cadaverine production
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Nachweismethoden von SARS‐CoV‐2
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Journal Articles

[Protocol]Rapid method for chromatin immunoprecipitation (ChIP) assay in a dimorphic fungus, Candida albicans: Jueun Kim , Jung-Shin Lee; J. Microbiol. 2020;58(1):11-16. Published online June 11, 2019; DOI: https://doi.org/10.1007/s12275-020-9143-2

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Abstract

A chromatin immunoprecipitation (ChIP) assay is a method to identify how much a protein of interest binds to the DNA region. This method is indispensable to study the mechanisms of how the transcription factors or chromatin modifications regulate the gene expression. Candida albicans is a dimorphic pathogenic fungus, which can change its morphology very rapidly from yeast to hypha in response to the environmental signal. The morphological change of C. albicans is one of the critical factors for its virulence. Therefore, it is necessary to understand how to regulate the expression of genes for C. albicans to change its morphology. One of the essential methods for us to understand this regulation is a ChIP assay. There have been many efforts to optimize the protocol to lower the background signal and to analyze the results accurately because a ChIP assay can provide very different results even with slight differences in the experimental procedure. We have optimized the rapid and efficient ChIP protocol so that it could be applied equally for both yeast and hyphal forms of C. albicans. Our method in this protocol is also comparatively rapid to the method widely used. In this protocol, we described our rapid method for the ChIP assay in C. albicans in detail.

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Transcription tuned by S-nitrosylation underlies a mechanism for Staphylococcus aureus to circumvent vancomycin killing
Xueqin Shu, Yingying Shi, Yi Huang, Dan Yu, Baolin Sun
Nature Communications.2023;[Epub] CrossRef
Molecular Identification, Dimorphism and Virulence of C. albicans
Mohsen A. Sayed, Gihad A. Sayed, Eman Abdullah M. Ali
Research Journal of Pharmacy and Technology.2023; : 1007. CrossRef
Methyltransferase-like 3 silenced inhibited the ferroptosis development via regulating the glutathione peroxidase 4 levels in the intracerebral hemorrhage progression
Liu Zhang, Xiangyu Wang, Wenqiang Che, Yongjun Yi, Shuoming Zhou, Yongjian Feng
Bioengineered.2022; 13(6): 14215. CrossRef
Ino80 is required for H2A.Z eviction from hypha‐specific promoters and hyphal development of Candida albicans
Qun Zhao, Baodi Dai, Hongyu Wu, Wencheng Zhu, Jiangye Chen
Molecular Microbiology.2022; 118(1-2): 92. CrossRef
Set1-mediated H3K4 methylation is required for Candida albicans virulence by regulating intracellular level of reactive oxygen species
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Virulence.2021; 12(1): 2648. CrossRef

Paraflavitalea soli gen. nov., sp. nov., isolated from greenhouse soil: Jun Heo , Hang-Yeon Weon , Hayoung Cho , Seung-Beom Hong , Jeong-Seon Kim , Soo-Jin Kim , Soon-Wo Kwon; J. Microbiol. 2020;58(1):17-23. Published online November 23, 2019; DOI: https://doi.org/10.1007/s12275-020-9236-y

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Abstract

A bacterial strain designated 5GH32-13T was isolated from greenhouse soil in Yongin-city, Republic of Korea. Cells were Gram-stain-negative, strictly aerobic, motile rods of two different shapes. The strain was catalase-positive and oxidasenegative. Flexirubin-like pigments were not detected. β-Carotene was produced. The strain grew in the range of 10–37°C (optimum of 28–30°C) and pH 6–8 (optimum of pH 7) and tolerated up to 1% (w/v) NaCl (optimum of 0%). According to the 16S rRNA gene sequence comparison, strain 5GH32- 13T shared a sequence similarity of less than 96.0% with all validly named taxa, having the highest sequence similarity with Pseudoflavitalea soli KIS20-3T (95.8%), Pseudoflavitalea rhizosphaerae T16R-265T (95.4%), Flavitalea gansuensis JCN-23T (95.3%), Pseudobacter ginsenosidimutans Gsoil 221T (95.3%), and Flavitalea populi HY-50RT (95.2%). A phylogenetic tree showed that strain 5GH32-13T was not grouped consistently into any specific genus. Its only polyamine was homospermidine, and its major fatty acids (> 10% of total fatty acids) were iso-C15:0, iso-C17:0 3-OH, and iso-C15:1 G. The strain’s only respiratory quinone was MK-7, and its polar lipids were phosphatidylethanolamine, one unidentified phospholipid, six unidentified aminolipids and four unidentified lipids. Its DNA G + C content was 47.5 mol%. The results from chemotaxonomic, phenotypic and phylogenetic analyses indicated that strain 5GH32-13T represents a novel species of a novel genus of the family Chitinophagaceae, and the name Paraflavitalea soli gen. nov., sp. nov. is proposed. The type strain is 5GH32-13T (= KACC 17331T = JCM 33061T).

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Lisanne Hameleers, Lucie A. Gaenssle, Salvador Bertran‐Llorens, Tjaard Pijning, Edita Jurak
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Paraflavitalea pollutisoli sp. nov., Pollutibacter soli gen. nov. sp. nov., Polluticoccus soli gen. nov. sp. nov., and Terrimonas pollutisoli sp. nov., four new members of the family Chitinophagaceae from polluted soil
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Ruiqing Wang, Zhihua Zhang, Fengjuan Lv, Hongxin Lin, Lingen Wei, Yunping Xiao
Rhizosphere.2022; 23: 100550. CrossRef
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Xiaoxiao Hou, Hongliang Liu, Yumang Shang, Sidi Mao, Shucheng Li, Feng Sang, Hongkuan Deng, Lijuan Wang, Ling Kong, ChunYang Zhang, Zhongfeng Ding, Yan Gao, Shuzhen Wei, Zhiwei Chen
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Saccharibacillus brassicae sp. nov., an endophytic bacterium isolated from kimchi cabbage (Brassica rapa subsp. pekinensis) seeds: Lingmin Jiang , Chan Ju Lim , Song-Gun Kim , Jae Cheol Jeong , Cha Young Kim , Dae-Hyuk Kim , Suk Weon Kim , Jiyoung Lee; J. Microbiol. 2020;58(1):24-29. Published online November 25, 2019; DOI: https://doi.org/10.1007/s12275-020-9346-6

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Abstract

Strain ATSA2T was isolated from surface-sterilized kimchi cabbage (Brassica rapa subsp. pekinensis) seeds and represents a novel bacterium based on the polyphasic taxonomic approach. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ATSA2T formed a lineage within genus Saccharibacillus and was most closely to Saccharibacillus deserti WLG055T (98.1%) and Saccharibacillus qingshengii H6T (97.9%). The whole-genome of ATSA2T comprised a 5,619,468 bp of circular chromosome with 58.4% G + C content. The DNA-DNA relatedness values between strain ATSA2T and its closely related type strains S. deserti WLJ055T and S. qingshengii H6T were 26.0% and 24.0%, respectively. Multiple gene clusters associated with plant growth promotion activities (stress response, nitrogen and phosphorus metabolism, and auxin biosynthesis) were annotated in the genome. Strain ATSA2T was Gram-positive, endospore-forming, facultatively anaerobic, and rod-shaped. It grew at 15–37°C (optimum 25°C), pH 6.0–10.0 (optimum pH 8.0), and in the presence of 0–5% (w/v) NaCl (optimum 1%). The major cellular fatty acids (> 10%) of strain ATSA2T were anteiso- C15:0 and C16:0. MK-7 was the major isoprenoid quinone. The major polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, and three unknown glycolipids. Based on its phylogenetic, genomic, phenotypic, and chemotaxonomic features, strain ATSA2T is proposed to represent a novel species of genus Saccharibacillus, for which the name is Saccharibacillus brassicae sp. nov. The type strain is ATSA2T (KCTC 43072T = CCTCC AB 2019223T).

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Yuxin Peng, Dong Hyun Cho, Zalfa Humaira, Yu Lim Park, Ki Hyun Kim, Cha Young Kim, Jiyoung Lee
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Dasania phycosphaerae sp. nov., isolated from phytoplankton sample from the south coast of the Republic of Korea
Yue Jiang, Yong Guan, Sungmo Kang, Mi-Kyung Lee, Ki-Hyun Kim, Zhun Li
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Genome insights into the plant growth-promoting bacterium Saccharibacillus brassicae ATSA2T
Lingmin Jiang, Jiyoon Seo, Yuxin Peng, Doeun Jeon, Soon Ju Park, Cha Young Kim, Pyoung Il Kim, Chul Hong Kim, Ju Huck Lee, Jiyoung Lee
AMB Express.2023;[Epub] CrossRef
Emticicia fluvialis sp. nov., a potential hormone-degrading bacterium isolated from Nakdong River, Republic of Korea
Hyun-Sun Baek, Yong Guan, Min-Ju Kim, Yue Jiang, Mi-Kyung Lee, Ki-Hyun Kim, Jaeyoon Lee, Yuna Shin, Yoon-Ho Kang, Zhun Li
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Identification and genomic analysis of Pseudosulfitobacter koreense sp. nov. isolated from toxin-producing dinoflagellate Alexandrium pacificum
Yue Jiang, Zhun Li
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Gymnodinialimonas phycosphaerae sp. nov., a phycosphere bacterium isolated from Karlodinium veneficum
Yuxin Peng, Lingmin Jiang, Yue Jiang, Jiyoon Seo, Doeun Jeon, Young-Min Kim, Zhun Li, Jiyoung Lee
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Flavobacterium endoglycinae sp. nov., an endophytic bacterium isolated from soybean (Glycine max L. cv. Gwangan) stems
Jiyoon Seo, Yuxin Peng, Lingmin Jiang, Sang-Beom Lee, Rae-Dong Jeong, Soon Ju Park, Cha Young Kim, Man-Soo Choi, Jiyoung Lee
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Gymnodinialimonas ceratoperidinii gen. nov., sp. nov., isolated from rare marine dinoflagellate Ceratoperidinium margalefii
Yue Jiang, Yuxin Peng, Hyeon Ho Shin, Hyun Jung Kim, Ki-Hyun Kim, Lingmin Jiang, Jiyoung Lee, Zhun Li
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Yue Jiang, Lingmin Jiang, Yuxin Peng, Ki-Hyun Kim, Hyeon Ho Shin, Young-Min Kim, Jiyoung Lee, Zhun Li
International Journal of Systematic and Evolutionary Microbiology .2021;[Epub] CrossRef
Pedobacter endophyticus sp. nov., an endophytic bacterium isolated from Carex pumila
Yuxin Peng, Lingmin Jiang, Jiyoon Seo, Zhun Li, Hanna Choe, Jae Cheol Jeong, Suk Weon Kim, Young-Min Kim, Cha Young Kim, Jiyoung Lee
International Journal of Systematic and Evolutionary Microbiology .2021;[Epub] CrossRef
Neobacillus endophyticus sp. nov., an endophytic bacterium isolated from Selaginella involvens roots
Lingmin Jiang, Myoung Hui Lee, Jae Cheol Jeong, Dae-Hyuk Kim, Cha Young Kim, Suk Weon Kim, Jiyoung Lee
International Journal of Systematic and Evolutionary Microbiology .2019;[Epub] CrossRef

Sterilization efficiency of pathogen-contaminated cottons in a laundry machine: Yoonjae Shin , Jungha Park , Woojun Park; J. Microbiol. 2020;58(1):30-38. Published online November 25, 2019; DOI: https://doi.org/10.1007/s12275-020-9391-1

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Abstract

Pathogenic bacteria on abiotic surfaces such as fabrics, bedding, patient wears, and surgical tools are known to increase the risk of bacterial diseases in infants and the elderly. The desiccation tolerance of bacteria affects their viability in cotton. Thus, washing and drying machines are required to use conditions that ensure the sterilization of bacteria in cotton. The objective of this study is to determine the effects of various sterilization conditions of washing and drying machines on the survival of three pathogenic bacteria (Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus) commonly presented in contaminated cotton and two non-pathogenic bacteria (Bacillus subtilis and Escherichia coli) in cotton. High survival rates of A. baumannii and S. aureus in desiccated cotton were observed based on scanning electron microscope and replicate organism direct agar contact assay. The survival rates of A. baumannii and S. aureus exposed in desiccated cotton for 8 h were higher (14.4 and 5.0%, respectively) than those of other bacteria (< 0.5%). All tested bacteria were eradicated at low-temperature (< 40°C) washing with activated oxygen bleach (AOB). However, bacterial viability was shown in low temperature washing without AOB. High-temperature (> 60°C) washing was required to achieve 99.9% of the sterilization rate in washing without AOB. The sterilization rate was 93.2% using a drying machine at 60°C for 4 h. This level of sterilization was insufficient in terms of time and energy efficiency. High sterilization efficiency (> 99.9%) at 75°C for 3 h using a drying machine was confirmed. This study suggests standard conditions of drying machines to remove bacterial contamination in cotton by providing practical data.

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Identification and characterization of a novel light-induced promoter for recombinant protein production in Pleurotus ostreatus: Chaomin Yin , Xiuzhi Fan , Kun Ma , Zheya Chen , Defang Shi , Fen Yao , Hong Gao , Aimin Ma; J. Microbiol. 2020;58(1):39-45. Published online November 4, 2019; DOI: https://doi.org/10.1007/s12275-020-9230-4

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Abstract

A lectin gene (plectin) with a high level of expression was previously identified by comparative transcriptome analysis of Pleurotus ostreatus. In this study, we cloned a 733-bp DNA fragment from the start codon of the plectin gene. Sequence analysis showed that the plectin promoter (Plp) region contained several eukaryotic transcription factor binding motifs, such as the TATA-box, four possible CAAT-box, light responsiveness motifs and MeJA-responsiveness motifs. To determine whether the Plp promoter was a light-regulated promoter, we constructed an expression vector with the fused egfp-hph fragment under the control of the Plp promoter and transformed P. ostreatus mycelia via Agrobacterium tumefaciens. PCR and Southern blot analyses confirmed the Plpegfp- hph fragment was integrated into the chromosomal DNA of transformants. qRT-PCR, egfp visualization, and intracellular egfp determination experiments showed the Plp promoter could be a light-induced promoter that may be suitable for P. ostreatus genetic engineering. This study lays the foundation for gene homologous expression in P. ostreatus.

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The GATA transcription factor BcWCL2 regulates citric acid secretion to maintain redox homeostasis and full virulence in Botrytis cinerea
Weiheng Ren, Chen Qian, Dandan Ren, Yunfei Cai, Zhaohui Deng, Ning Zhang, Congcong Wang, Yiwen Wang, Pinkuan Zhu, Ling Xu, Regine Kahmann
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Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate: Woo-Chang Chung , Kwang Yeon Hwang , Suk-Jo Kang , Jae-Ouk Kim , Moon Jung Song; J. Microbiol. 2020;58(1):46-53. Published online November 25, 2019; DOI: https://doi.org/10.1007/s12275-020-9384-0

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Abstract

The Chikungunya virus (CHIKV) belongs to the Alphavirus genus of Togaviridae family and contains a positive-sense single stranded RNA genome. Infection by this virus mainly causes sudden high fever, rashes, headache, and severe joint pain that can last for several months or years. CHIKV, a mosquito- borne arbovirus, is considered a re-emerging pathogen that has become one of the most pressing global health concerns due to a rapid increase in epidemics. Because handling of CHIKV is restricted to Biosafety Level 3 (BSL-3) facilities, the evaluation of prophylactic vaccines or antivirals has been substantially hampered. In this study, we first identified the whole structural polyprotein sequence of a CHIKV strain isolated in South Korea (KNIH/2009/77). Phylogenetic analysis showed that this sequence clustered within the East/ Central/South African CHIKV genotype. Using this sequence information, we constructed a CHIKV-pseudotyped lentivirus expressing the structural polyprotein of the Korean CHIKV isolate (CHIKVpseudo) and dual reporter genes of green fluorescence protein and luciferase. We then developed a pseudovirus-based neutralization assay (PBNA) using CHIKVpseudo. Results from this assay compared to those from the conventional plaque reduction neutralization test showed that our PBNA was a reliable and rapid method to evaluate the efficacy of neutralizing antibodies. More importantly, the neutralizing activities of human sera from CHIKVinfected individuals were quantitated by PBNA using CHIKVpseudo. Taken together, these results suggest that our PBNA for CHIKV may serve as a useful and safe method for testing the neutralizing activity of antibodies against CHIKV in BSL-2 facilities.

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Scientific Reports.2022;[Epub] CrossRef
Prevalence of Malaria and Chikungunya Co-Infection in Febrile Patients: A Systematic Review and Meta-Analysis
Wanida Mala, Polrat Wilairatana, Kwuntida Uthaisar Kotepui, Manas Kotepui
Tropical Medicine and Infectious Disease.2021; 6(3): 119. CrossRef

Human cytomegalovirus IE86 protein aa 136–289 mediates STING degradation and blocks the cGAS-STING pathway: Jun-Kyu Lee , Jung-Eun Kim , Bang Ju Park , Yoon-Jae Song; J. Microbiol. 2020;58(1):54-60. Published online January 2, 2020; DOI: https://doi.org/10.1007/s12275-020-9577-6

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Abstract

We previously reported that human cytomegalovirus (HCMV) 86 kDa immediate-early 2 gene product (IE86) promotes proteasome-dependent degradation of STING. In the present study, we determined the specific residues of IE86 responsible for STING degradation using a STING-firefly luciferase fusion protein expression system for quantitative measurement of STING protein levels. IE86 amino acids (aa) 136–289 were sufficient to promote STING degradation and further induced down-regulation of 2􍿁3􍿁-cyclic GMP-AMP (cGAMP)-mediated IFN-β promoter activation. Interestingly, transactivation domains (TAD) of the IE86 protein located at the N- and C-termini were required for down-regulation of Toll/interleukin-1 receptor (TIR) domain-containing adaptor- inducing interferon β (IFN-β) (TRIF)-mediated IFN-β- and p65/RelA-induced NF-κB-dependent promoter activation while amino acids (aa) 136–289 had no significant effects. Our collective data suggest that the IE86 protein utilizes the aa 136–289 region to promote STING degradation and inhibit the cGAS-STING pathway.

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IUPHAR ECR review: The cGAS-STING pathway: Novel functions beyond innate immune and emerging therapeutic opportunities
Xu He, Abdalla Wedn, Jian Wang, Yanlun Gu, Hongjin Liu, Juqi Zhang, Zhiqiang Lin, Renpeng Zhou, Xiaocong Pang, Yimin Cui
Pharmacological Research.2024; 201: 107063. CrossRef
The battle between host antiviral innate immunity and immune evasion by cytomegalovirus
Shuang Li, Yuanyang Xie, Changyin Yu, Chunfu Zheng, Zucai Xu
Cellular and Molecular Life Sciences.2024;[Epub] CrossRef
Human cytomegalovirus: pathogenesis, prevention, and treatment
Zifang Shang, Xin Li
Molecular Biomedicine.2024;[Epub] CrossRef
Sensing of viral lung infections by cGAS-STING
Lei Fang, Michael Roth
Exploration of Immunology.2022; : 303. CrossRef
Advances in cGAS-STING Signaling Pathway and Diseases
Yuting Yang, Yiming Huang, Zhenguo Zeng
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Evasion of the Host Immune Response by Betaherpesviruses
Daniel Sausen, Kirstin Reed, Maimoona Bhutta, Elisa Gallo, Ronen Borenstein
International Journal of Molecular Sciences.2021; 22(14): 7503. CrossRef
Regulation of antiviral innate immune signaling and viral evasion following viral genome sensing
Kiramage Chathuranga, Asela Weerawardhana, Niranjan Dodantenna, Jong-Soo Lee
Experimental & Molecular Medicine.2021; 53(11): 1647. CrossRef
Zika virus NS2A inhibits interferon signaling by degradation of STAT1 and STAT2
Elisa Fanunza, Fabrizio Carletti, Marina Quartu, Nicole Grandi, Laura Ermellino, Jessica Milia, Angela Corona, Maria Rosaria Capobianchi, Giuseppe Ippolito, Enzo Tramontano
Virulence.2021; 12(1): 1580. CrossRef
Human Cytomegalovirus Immediate Early Protein 2 Protein Causes Cognitive Disorder by Damaging Synaptic Plasticity in Human Cytomegalovirus-UL122-Tg Mice
Zhifei Wang, Wenwen Yu, Lili Liu, Junyun Niu, Xianjuan Zhang, Fulong Nan, Lili Xu, Bin Jiang, Dingxin Ke, Wenhua Zhu, Zibin Tian, Yashuo Wang, Bin Wang
Frontiers in Aging Neuroscience.2021;[Epub] CrossRef

Repositioning of a mucolytic drug to a selective antibacterial against Vibrio cholerae: In-Young Chung† , Bi-o Kim† , Hye-Jeong Jang† , You-Hee Cho; J. Microbiol. 2020;58(1):61-66. Published online January 2, 2020; DOI: https://doi.org/10.1007/s12275-020-9590-9

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Abstract

Drug repositioning, the approach to explore existing drugs for use in new therapeutic indications, has emerged as an alternative drug development strategy. In this study, we found that a mucolytic drug, N-acetylcysteine (NAC) showed antibacterial activity against Vibrio cholerae. NAC can provide acid stress that selectively inhibited the growth of V. cholerae among other bacterial pathogens. To address the antibacterial mechanism of NAC against V. cholerae, six acr (acetylcysteine- resistant) mutants were isolated from 3,118 random transposon insertion clones. The transposon insertion sites of the six mutants were mapped at the five genes. All these mutants did not display NAC resistance under acidic conditions, despite their resistance to NAC under alkaline conditions, indicating that the NAC resistance directed by the acr mutations was independent of the unusual pH-sensitivity of V. cholerae. Furthermore, all these mutants displayed attenuated virulence and reduced biofilm formation, suggesting that the acr genes are required for pathogenesis of V. cholerae. This study validates the relevance of drug repositioning for antibacterials with new modes of action and will provide an insight into a novel antibacterial therapy for V. cholerae infections to minimize side effects and resistance emergence.

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Identification of brevinin-1EMa-derived stapled peptides as broad-spectrum virus entry blockers
Mi Il Kim, Thanh K. Pham, Dahee Kim, Minkyung Park, Bi-o Kim, You-Hee Cho, Young-Woo Kim, Choongho Lee
Virology.2021; 561: 6. CrossRef

Zur-regulated lipoprotein A contributes to the fitness of Acinetobacter baumannii: Eun Kyung Lee , Chul Hee Choi , Man Hwan Oh; J. Microbiol. 2020;58(1):67-77. Published online January 2, 2020; DOI: https://doi.org/10.1007/s12275-020-9531-7

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Abstract

Acinetobacter baumannii is a notorious nosocomial pathogen that commonly infects severely ill patients. Zinc (Zn) is essential to survive and adapt to different environment and host niches in A. baumannii. Of the Zinc uptake regulator (Zur)-regulated genes in A. baumannii, the A1S_3412 gene encoding a Zur-regulated lipoprotein A (ZrlA) is critical for cell envelope integrity and overcoming antibiotic exposure. This study investigated whether ZrlA contributes to the fitness of A. baumannii in vitro and in vivo using the wildtype A. baumannii ATCC 17978, ΔzrlA mutant, and zrlAcomplemented strains. The ΔzrlA mutant showed reduced biofilm formation, surface motility, and adherence to and invasion of epithelial cells compared to the wild-type strain. In a mouse pneumonia model, the ΔzrlA mutant showed significantly lower bacterial numbers in the blood than the wildtype strain. These virulence traits were restored in the zrlAcomplemented strain. Under static conditions, the expression of csuCDE, which are involved in the chaperone-usher pili assembly system, was significantly lower in the ΔzrlA mutant than in the wild-type strain. Moreover, the expression of the bfmR/S genes, which regulate the CsuA/BABCDE system, was significantly lower in the ΔzrlA mutant under static conditions than in the wild-type strain. Our results indicate that the zrlA gene plays a role in the fitness of A. baumannii by regulating the BfmR/S two-component system and subsequently the CsuA/BABCDE chaperone-usher pili assembly system, suggesting it as a potential target for anti-virulence strategies against A. baumannii.

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Molecular Detection of Pap II, OmpA, and LuxR Genes Responsible for Biofilm Formation in Acinetobacter baumannii Isolated from Hospitalized Patients
Estabraq Ali Maklef, Amal A. Kareem, Susan F. K. Al-Sudani
Medical Journal of Babylon.2024; 21(Suppl 2): S258. CrossRef
Pathogenicity and virulence of Acinetobacter baumannii : Factors contributing to the fitness in healthcare settings and the infected host
Massimiliano Lucidi, Daniela Visaggio, Antonella Migliaccio, Giulia Capecchi, Paolo Visca, Francesco Imperi, Raffaele Zarrilli
Virulence.2024;[Epub] CrossRef
Characterization of the Zinc Uptake Repressor (Zur) from Acinetobacter baumannii
Minyong Kim, My Tra Le, Lixin Fan, Courtney Campbell, Sambuddha Sen, Daiana A. Capdevila, Timothy L. Stemmler, David P. Giedroc
Biochemistry.2024; 63(5): 660. CrossRef
Acinetobacter Metabolism in Infection and Antimicrobial Resistance
Xiaomei Ren, Lauren D. Palmer, Karen M. Ottemann
Infection and Immunity.2023;[Epub] CrossRef
A response regulator controls Acinetobacter baumannii virulence by acting as an indole receptor
Binbin Cui, Quan Guo, Xia Li, Shihao Song, Mingfang Wang, Gerun Wang, Aixin Yan, Jianuan Zhou, Yinyue Deng, Marenda Wilson-Pham
PNAS Nexus.2023;[Epub] CrossRef
The role of quorum sensing, biofilm formation, and iron acquisition as key virulence mechanisms in Acinetobacter baumannii and the corresponding anti-virulence strategies
Soffi Kei Kei Law, Hock Siew Tan
Microbiological Research.2022; 260: 127032. CrossRef
Carboxy-Terminal Processing Protease Controls Production of Outer Membrane Vesicles and Biofilm in Acinetobacter baumannii
Rakesh Roy, Ren-In You, Chan-Hua Chang, Chiou-Ying Yang, Nien-Tsung Lin
Microorganisms.2021; 9(6): 1336. CrossRef
ppGpp signaling plays a critical role in virulence of Acinetobacter baumannii
Kyeongmin Kim, Maidul Islam, Hye-won Jung, Daejin Lim, Kwangsoo Kim, Sung-Gwon Lee, Chungoo Park, Je Chul Lee, Minsang Shin
Virulence.2021; 12(1): 2122. CrossRef
COG0523 proteins: a functionally diverse family of transition metal-regulated G3E P-loop GTP hydrolases from bacteria to man
Katherine A Edmonds, Matthew R Jordan, David P Giedroc
Metallomics.2021;[Epub] CrossRef
The role of Zur-regulated lipoprotein A in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles in Acinetobacter baumannii
Nayeong Kim, Hyo Jeong Kim, Man Hwan Oh, Se Yeon Kim, Mi Hyun Kim, Joo Hee Son, Seung Il Kim, Minsang Shin, Yoo Chul Lee, Je Chul Lee
BMC Microbiology.2021;[Epub] CrossRef
Insights Into Mechanisms of Biofilm Formation in Acinetobacter baumannii and Implications for Uropathogenesis
Jennifer M. Colquhoun, Philip N. Rather
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef

Published Erratums

Erratum]Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology: Kui Wu , Yangyun Zheng , Qingping Wu , Haiying Chen , Songzhe Fu , Biao Kan , Yongyan Long , Xiansheng Ni , Junling Tu; J. Microbiol. 2020;58(1):78-78.; DOI: https://doi.org/10.1007/s12275-020-9721-3

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Abstract: In the article by Wu et al. published in Journal of Microbiology 2019; 57, 1105–1114, the figure 8 is unfortunately incorrect. The figure 8 should be corrected as below. We apologize for any inconvenience that this may have caused.

Erratum]Methylobacterium terrae sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil: Jiyoun Kim , Geeta Chhetri , Inhyup Kim , Hyungdong Kim , Myung Kyum Kim , Taegun Seo; J. Microbiol. 2020;58(1):79-79.; DOI: https://doi.org/10.1007/s12275-020-9722-2

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Abstract

In the article by Kim et al. published in Journal of Microbiology 2019; 57, 959–966, The NBRC accession number NBRC 112879T on 33th line of 2nd paragraph in the section of ‘Description of Methylobacterium terrae sp. nov.’ on page 964 should be corrected in NBRC 112873T. The sentence in abstract should have read: The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and genome sequences of the type strain 17Sr1-28T (= KCTC 52904T = NBRC 112873T) are KY939566 and CP029553, respectively. We apologize for any inconvenience that this may have caused.

Citations

Citations to this article as recorded by

International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Rhizobia and Agrobacteria Minutes of the closed meeting by videoconference, 6 July 2020
Philippe de Lajudie, Seyed Abdollah Mousavi, J. Peter W. Young
International Journal of Systematic and Evolutionary Microbiology .2021;[Epub] CrossRef
International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Rhizobia and Agrobacteria Minutes of the closed meeting by videoconference, 17 July 2019
Philippe de Lajudie, J. Peter W. Young
International Journal of Systematic and Evolutionary Microbiology .2020; 70(5): 3563. CrossRef

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