- Volume 52(2); February 2014
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Review
- MINIREVIEW] To Peep into Pif1 Helicase: Multifaceted All the Way from Genome Stability to Repair-Associated DNA Synthesis
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Woo-Hyun Chung
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J. Microbiol. 2014;52(2):89-98. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3524-3
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Abstract
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Pif1 DNA helicase is the prototypical member of a 5' to 3' helicase superfamily conserved from bacteria to humans. In Saccharomyces cerevisiae, Pif1 and its homologue Rrm3, localize in both mitochondria and nucleus playing multiple roles in the maintenance of genomic homeostasis. They display relatively weak processivities in vitro, but have largely non-overlapping functions on common genomic loci such as mitochondrial DNA, telomeric ends, and many replication forks especially at hard-to-replicate regions including ribosomal DNA and G-quadruplex structures. Recently, emerging evidence shows that Pif1, but not Rrm3, has a significant new role in repair-associated DNA synthesis with Polδ during homologous recombination stimulating D-loop migration for conservative DNA replication. Comparative genetic and biochemical studies on the structure and function of Pif1 family helicases across different biological systems are further needed to elucidate both diversity and specificity of their mechanisms of action that contribute to genome stability.
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Citations
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- Signification and Application of Mutator and Antimutator Phenotype-Induced Genetic Variations in Evolutionary Adaptation and Cancer Therapeutics
Woo-Hyun Chung
Journal of Microbiology.2023; 61(12): 1013. CrossRef - The Pif1 helicase is actively inhibited during meiotic recombination which restrains gene conversion tract length
Dipti Vinayak Vernekar, Giordano Reginato, Céline Adam, Lepakshi Ranjha, Florent Dingli, Marie-Claude Marsolier, Damarys Loew, Raphaël Guérois, Bertrand Llorente, Petr Cejka, Valérie Borde
Nucleic Acids Research.2021; 49(8): 4522. CrossRef - Approaching Protein Barriers: Emerging Mechanisms of Replication Pausing in Eukaryotes
Maksym Shyian, David Shore
Frontiers in Cell and Developmental Biology.2021;[Epub] CrossRef - Structural and functional studies of SF1B Pif1 from Thermus oshimai reveal dimerization-induced helicase inhibition
Yang-Xue Dai, Wei-Fei Chen, Na-Nv Liu, Fang-Yuan Teng, Hai-Lei Guo, Xi-Miao Hou, Shuo-Xing Dou, Stephane Rety, Xu-Guang Xi
Nucleic Acids Research.2021; 49(7): 4129. CrossRef - When the Ends Justify the Means: Regulation of Telomere Addition at Double-Strand Breaks in Yeast
Remington E. Hoerr, Katrina Ngo, Katherine L. Friedman
Frontiers in Cell and Developmental Biology.2021;[Epub] CrossRef - Lysine acetylation regulates the activity of nuclear Pif1
Onyekachi E. Ononye, Christopher W. Sausen, Lata Balakrishnan, Matthew L. Bochman
Journal of Biological Chemistry.2020; 295(46): 15482. CrossRef - Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family
Julius Muellner, Kristina H. Schmidt
Genes.2020; 11(2): 224. CrossRef - Two Pif1 Family DNA Helicases Cooperate in Centromere Replication and Segregation in Saccharomyces cerevisiae
Chi-Fu Chen, Thomas J Pohl, Sebastian Pott, Virginia A Zakian
Genetics.2019; 211(1): 105. CrossRef - Ku complex suppresses recombination in the absence of MRX activity during budding yeast meiosis
Hyeseon Yun, Keunpil Kim
BMB Reports.2019; 52(10): 607. CrossRef - Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases
Nannan Su, Alicia K. Byrd, Sakshibeedu R. Bharath, Olivia Yang, Yu Jia, Xuhua Tang, Taekjip Ha, Kevin D. Raney, Haiwei Song
Nature Communications.2019;[Epub] CrossRef - The nature of meiotic chromosome dynamics and recombination in budding yeast
Soogil Hong, Jeong Hwan Joo, Hyeseon Yun, Keunpil Kim
Journal of Microbiology.2019; 57(4): 221. CrossRef - The Drosophila melanogaster PIF1 Helicase Promotes Survival During Replication Stress and Processive DNA Synthesis During Double-Strand Gap Repair
Ece Kocak, Sarah Dykstra, Alexandra Nemeth, Catherine G Coughlin, Kasey Rodgers, Mitch McVey
Genetics.2019; 213(3): 835. CrossRef - The signature motif of the Saccharomyces cerevisiae Pif1 DNA helicase is essential in vivo for mitochondrial and nuclear functions and in vitro for ATPase activity
Carly L Geronimo, Saurabh P Singh, Roberto Galletto, Virginia A Zakian
Nucleic Acids Research.2018; 46(16): 8357. CrossRef - DNA-unwinding activity of Saccharomyces cerevisiae Pif1 is modulated by thermal stability, folding conformation, and loop lengths of G-quadruplex DNA
Lei Wang, Qing-Man Wang, Yi-Ran Wang, Xu-Guang Xi, Xi-Miao Hou
Journal of Biological Chemistry.2018; 293(48): 18504. CrossRef - Role of the Pif1-PCNA Complex in Pol δ-Dependent Strand Displacement DNA Synthesis and Break-Induced Replication
Olga Buzovetsky, Youngho Kwon, Nhung Tuyet Pham, Claire Kim, Grzegorz Ira, Patrick Sung, Yong Xiong
Cell Reports.2017; 21(7): 1707. CrossRef - Structure and function of Pif1 helicase
Alicia K. Byrd, Kevin D. Raney
Biochemical Society Transactions.2017; 45(5): 1159. CrossRef - Mechanistic and biological considerations of oxidatively damaged DNA for helicase-dependent pathways of nucleic acid metabolism
Jack D. Crouch, Robert M. Brosh
Free Radical Biology and Medicine.2017; 107: 245. CrossRef - PIF1 family DNA helicases suppress R-loop mediated genome instability at tRNA genes
Phong Lan Thao Tran, Thomas J. Pohl, Chi-Fu Chen, Angela Chan, Sebastian Pott, Virginia A. Zakian
Nature Communications.2017;[Epub] CrossRef - Yeast Helicase Pif1 Unwinds RNA:DNA Hybrids with Higher Processivity than DNA:DNA Duplexes
Shubeena Chib, Alicia K. Byrd, Kevin D. Raney
Journal of Biological Chemistry.2016; 291(11): 5889. CrossRef - Getting it done at the ends: Pif1 family DNA helicases and telomeres
Carly L. Geronimo, Virginia A. Zakian
DNA Repair.2016; 44: 151. CrossRef - Genetic instability in budding and fission yeast—sources and mechanisms
Adrianna Skoneczna, Aneta Kaniak, Marek Skoneczny, Antoine Danchin
FEMS Microbiology Reviews.2015; 39(6): 917. CrossRef - TheBacteroides sp. 3_1_23Pif1 protein is a multifunctional helicase
Na-Nv Liu, Xiao-Lei Duan, Xia Ai, Yan-Tao Yang, Ming Li, Shuo-Xing Dou, Stephane Rety, Eric Deprez, Xu-Guang Xi
Nucleic Acids Research.2015; 43(18): 8942. CrossRef - Yeast Pif1 Accelerates Annealing of Complementary DNA Strands
Ramanagouda Ramanagoudr-Bhojappa, Alicia K. Byrd, Christopher Dahl, Kevin D. Raney
Biochemistry.2014; 53(48): 7659. CrossRef
Research Support, Non-U.S. Gov'ts
- Comparative Phylogenetic Relationships and Genetic Structure of the Caterpillar Fungus Ophiocordyceps sinensis and Its Host Insects Inferred from Multiple Gene Sequences
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Qing-Mei Quan , Qing-Xia Wang , Xue-Li Zhou , Shan Li , Xiao-Ling Yang , Yun-Guo Zhu , Zhou Cheng
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J. Microbiol. 2014;52(2):99-105. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3391-y
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Abstract
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Ophiocordyceps sinensis (Ascomycota: Ophiocordycipitaceae) is a native fungal parasite of Hepialidae caterpillars and one of the most economically important medicinal caterpillar fungi in China. However, little is known about the phylogenetic and evolutionary relationships between O. sinensis and its host insects. In this study, nuclear ITS and β-tubulin sequences from O. sinensis and mitochondrial COI, COII, and Cytb sequences from its hosts were analyzed across 33 populations sampled from five regions in China. Phylogenetically, both O. sinensis and its hosts were divided into three geographically correlated clades, and their phylogenies were congruent. Analysis of molecular variance and calculated coefficients of genetic differentiation revealed significant genetic divergence among the clades within both O. sinensis (FST=0.878, NST=0.842) and its hosts (FST=0.861, NST=0.816). Estimated gene flow was very low for O. sinensis (Nm=0.04) and the host insects (Nm=0.04) among these three clades. Mantel tests demonstrated a significant correlation (P<0.01) between the genetic distances for O. sinensis and its hosts, as well as a significant association (P<0.05) between geographic and genetic distances in both. The similar phylogenetic relationships, geographic distributions, and genetic structure and differentiation between O. sinensis and its hosts imply that they have coevolved.
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- A Review: Ophiocordycep Sinensis (Berk.) as a Traditional Tibetan Medicine and its Potential in the Treatment of Various Human Ailments
Yangchen Dolma Kom, R. Karthiyayini
Current Traditional Medicine.2024;[Epub] CrossRef - The phylogeny and divergence time of Ophiocordyceps sinensis and its host insects based on elongation factor 1 alpha
Shan Li, Yunguo Zhu, Zixian Xu, Lingling Chen, Wenqian Wang, Zhou Cheng
Archives of Microbiology.2023;[Epub] CrossRef - Profiling, monitoring and conserving caterpillar fungus in the Himalayan region using anchored hybrid enrichment markers
Zhengyang Wang, Wa Da, Chandra Singh Negi, Puspa Lal Ghimire, Karma Wangdi, Pramod K. Yadav, Zhuoma Pubu, Laiku Lama, Kuenga Yarpel, Sarah C. Maunsell, Yong Liu, Krushnamegh Kunte, Kamaljit S. Bawa, Darong Yang, Naomi E. Pierce
Proceedings of the Royal Society B: Biological Sciences.2022;[Epub] CrossRef - Interspecific Hybridization and Complete Mitochondrial Genome Analysis of Two Ghost Moth Species
Hua Wu, Li Cao, Meiyu He, Richou Han, Patrick De Clercq
Insects.2021; 12(11): 1046. CrossRef - Conserving the Chinese caterpillar fungus under climate change
Yi Li, Yujing Yan, Zhiyao Tang, Ke Wang, Jin-Sheng He, Yi-Jian Yao
Biodiversity and Conservation.2021; 30(2): 547. CrossRef - Contrive Himalayan Soft Gold Cordyceps Species: a Lineage of Eumycota Bestowing Tremendous Pharmacological and Therapeutic Potential
Loknath Deshmukh, Anil K. Sharma, Sardul Singh Sandhu
Current Pharmacology Reports.2020; 6(4): 155. CrossRef - Effects of fungus–host associations on nucleoside differences among Ophiocordyceps sinensis populations on the Qinghai–Tibet Plateau of China
Zixian Xu, Shan Li, Lingling Chen, Yunguo Zhu, Lingyan Xuan, Zhou Cheng
Archives of Microbiology.2020; 202(8): 2323. CrossRef - Haplotype Diversity of NADPH-Cytochrome P450 Reductase Gene of Ophiocordyceps sinensis and the Effect on Fungal Infection in Host Insects
Zixian Xu, Yunguo Zhu, Lingyan Xuan, Shan Li, Zhou Cheng
Microorganisms.2020; 8(7): 968. CrossRef - Thitarodes shambalaensis sp. nov. (Lepidoptera, Hepialidae): a new host of the caterpillar fungus Ophiocordyceps sinensis supported by genome-wide SNP data
Zhengyang Wang, Hailing Zhuang, Min Wang, Naomi E. Pierce
ZooKeys.2019; 885: 89. CrossRef - Investigation on natural resources and species conservation of Ophiocordyceps sinensis, the famous medicinal fungus endemic to the Tibetan Plateau
Wenjing Wang, Ke Wang, Xiaoliang Wang, Ruiheng Yang, Yi Li, Yijian Yao
Protein & Cell.2018; 9(8): 671. CrossRef - Habitat Ecology of Ophiocordyceps sinensis in Western Nepal
Shalik R. Sigdel, Maan B. Rokaya, Zuzana Münzbergová, Eryuan Liang
Mountain Research and Development.2017; 37(2): 216. CrossRef - Detection of Ophiocordyceps sinensis and Its Common Adulterates Using Species-Specific Primers
Yang Liu, Xiao-yue Wang, Zi-tong Gao, Jian-ping Han, Li Xiang
Frontiers in Microbiology.2017;[Epub] CrossRef - Cordyceps collected from Bhutan, an appropriate alternative of Cordyceps sinensis
Ding-Tao Wu, Guang-Ping Lv, Jian Zheng, Qian Li, Shuang-Cheng Ma, Shao-Ping Li, Jing Zhao
Scientific Reports.2016;[Epub] CrossRef - The intronic minisatellite OsMin1 within a serine protease gene in the Chinese caterpillar fungus Ophiocordyceps sinensis
Yong-Jie Zhang, Jun-Xiu Hou, Shu Zhang, Georg Hausner, Xing-Zhong Liu, Wen-Jia Li
Applied Microbiology and Biotechnology.2016; 100(8): 3599. CrossRef - Pyrosequencing analysis revealed complex endogenetic microorganism community from natural DongChong XiaCao and its microhabitat
Fei Xia, Yan Liu, Meng-Yuan Guo, Guang-Rong Shen, Juan Lin, Xuan-Wei Zhou
BMC Microbiology.2016;[Epub] CrossRef - Enhancement of cordyceps polysaccharide production via biosynthetic pathway analysis in Hirsutella sinensis
Shan Lin, Zhi-Qiang Liu, Peter James Baker, Ming Yi, Hui Wu, Feng Xu, Yi Teng, Yu-Guo Zheng
International Journal of Biological Macromolecules.2016; 92: 872. CrossRef - Complete mitochondrial genome of the medicinal fungus Ophiocordyceps sinensis
Yi Li, Xiao-Di Hu, Rui-Heng Yang, Tom Hsiang, Ke Wang, De-Quan Liang, Fan Liang, De-Ming Cao, Fan Zhou, Ge Wen, Yi-Jian Yao
Scientific Reports.2015;[Epub] CrossRef - Diversity, population genetics, and evolution of macrofungi associated with animals
Xiaozhao Tang, Fei Mi, Ying Zhang, Xiaoxia He, Yang Cao, Pengfei Wang, Chunli Liu, Dan Yang, Jianyong Dong, Keqing Zhang, Jianping Xu
Mycology.2015; 6(2): 94. CrossRef - Phylogeography and evolution of a fungal–insect association on theTibetanPlateau
Yongjie Zhang, Shu Zhang, Yuling Li, Shaoli Ma, Chengshu Wang, Meichun Xiang, Xin Liu, Zhiqiang An, Jianping Xu, Xingzhong Liu
Molecular Ecology.2014; 23(21): 5337. CrossRef - Genetic Diversity and Distribution Patterns of Host Insects of Caterpillar Fungus Ophiocordyceps sinensis in the Qinghai-Tibet Plateau
Qing-Mei Quan, Ling-Ling Chen, Xi Wang, Shan Li, Xiao-Ling Yang, Yun-Guo Zhu, Mu Wang, Zhou Cheng, Dee A. Carter
PLoS ONE.2014; 9(3): e92293. CrossRef
- Paenibacillus swuensis sp. nov., a Bacterium Isolated from Soil
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Jae-Jin Lee , Da-Hye Yang , Ye-Sil Ko , Jae-Kyoung Park , Eun-Young Im , Ju-Yeon Kim , Ka-Young Kwon , Yu-Jung Lee , Hyung-Mi Kim , Myung Kyum Kim
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J. Microbiol. 2014;52(2):106-110. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3546-x
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Abstract
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Strain DY6T, a Gram-positive endospore-forming motile rodshaped bacterium, was isolated from soil in South Korea and characterized to determine its taxonomic position. Phylogenetic analyses based on the 16S rRNA gene sequence of strain DY6T revealed that strain DY6T belongs to the genus Paenibacillus in the family Paenibacillaceae in the class Bacilli. The highest degree of sequence similarities of strain DY6T were found with Paenibacillus gansuensis B518T (97.9%), P. chitinolyticus IFO 15660T (95.3%), P. chinjuensis WN9T (94.7%), and P. rigui WPCB173T (94.7%). Chemotaxonomic data revealed that the predominant fatty acids were anteiso-C15:0 (38.7%) and C16:0 (18.0%). A complex polar lipid profile consisted of major amounts of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The predominant respiratory quinone was MK-7. Based on these phylogenetic, chemotaxonomic, and phenotypic data, strain DY6T (=KCTC 33026T =JCM 18491T) should be classified as a type strain of a novel species, for which the name Paenibacillus swuensis sp. nov. is proposed.
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- Paenibacillus arcticus sp. nov., isolated from Arctic soil
In-Tae Cha, Eui-Sang Cho, Yesol Yoo, Yoon Ji Seok, Inhye Park, Hee Seon Lim, Jung-Min Park, Seong Woon Roh, Young-Do Nam, Hak-Jong Choi, Yoo Kyung Lee, Myung-Ji Seo
International Journal of Systematic and Evolutionary Microbiology.2017; 67(11): 4385. CrossRef - Paenibacillus albidus sp. nov., isolated from grassland soil
Junli Zhuang, Di Xin, Yu-Qin Zhang, Jingnan Guo, Jianli Zhang
International Journal of Systematic and Evolutionary Microbiology.2017; 67(11): 4685. CrossRef - Complete genome sequence of Paenibacillus swuensis DY6T, a bacterium isolated from gamma-ray irradiated soil
Myung Kyum Kim, Seung-Yeol Lee, Hee-Young Jung, Sathiyaraj Srinivasan
The Korean Journal of Microbiology.2016; 52(4): 500. CrossRef - Paenibacillus baekrokdamisoli sp. nov., isolated from soil of crater lake
Keun Chul Lee, Kwang Kyu Kim, Jong-Shik Kim, Dae-Shin Kim, Suk-Hyung Ko, Seung-Hoon Yang, Jung-Sook Lee
International Journal of Systematic and Evolutionary Microbiology.2016; 66(5): 1937. CrossRef - List of new names and new combinations previously effectively, but not validly, published
A. Oren, G. M. Garrity
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY.2015; 65(Pt 3): 741. CrossRef
- Microbial Communities in Semi-consolidated Carbonate Sediments of the Southwest Indian Ridge
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Jiwei Li , Xiaotong Peng , Huaiyang Zhou , Jiangtao Li , Zhilei Sun , Shun Chen
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J. Microbiol. 2014;52(2):111-119. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3133-1
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Abstract
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White semi-consolidated carbonate sediments attached to black ferromanganese oxide films were collected approximately 50 km west of a newly discovered hydrothermal field near the Southwest Indian Ridge (SWIR). The biodiversity of the prokaryotic communities within the field was examined using clone library-based culture-independent analysis of the exterior black oxides and the interior white carbonates. Subsequent 16S rRNA gene analysis suggested that Gammaproteobacteria, Acidobacteria, and Thaumarchaeota members dominated the bacterial and archaeal clone libraries. To further characterize the metabolic processes within the microbial community, analyses of the amoA (coding the alpha subunit of the ammonia monooxygenase for Archaea) and aprA (coding the alpha subunit of the dissimilatory adenosine-5'-phosphosulfate reductase for the sulfate-reducing and sulfur-oxidizing prokaryotes) functional genes were conducted. The functional gene analysis results suggested that Thaumarchaeota and Alphaproteobacteria members were the potential players that participated in N and S cycles in this marine carbonate sedimentary environment. This paper is the first to describe the microbial communities and their potential metabolic pathways within the semi-consolidated carbonate sediments of the SWIR.
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- An insight into the prokaryotic diversity from a polymetallic nodule-rich region in the Central Indian Ocean Basin using next generation sequencing approach
Shruti Shah, Samir R. Damare, Maria Brenda Luzia Mascarenhas-Pereira, Jayesh Patil, Sneha Parab, Sushil Nair, Arpita Ghosh
Frontiers in Microbiology.2024;[Epub] CrossRef - Biogeography and potential ecological functions of prokaryotes in the hydrothermal and non-hydrothermal field sediments of the Indian Ocean Ridges
Jing Huang, Ping Chen, Yaxin Zhu, Jian Wang, Lei Song, Xiqiu Han, Ying Huang
Frontiers in Marine Science.2023;[Epub] CrossRef - Spatiotemporal dynamics of high and low nucleic acid-content bacterial communities in Chinese coastal seawater: assembly process, co-occurrence relationship and the ecological functions
Wei Hu, Ningning Zheng, Yadi Zhang, Mark Bartlam, Yingying Wang
Frontiers in Microbiology.2023;[Epub] CrossRef - Impact of arsenic on microbial community structure and their metabolic potential from rice soils of West Bengal, India
Himadri Bose, Rajendra Prasad Sahu, Pinaki Sar
Science of The Total Environment.2022; 841: 156486. CrossRef - Microbial diversity and community structure in deep-sea sediments of South Indian Ocean
Daochen Zhu, Sivasamy Sethupathy, Lu Gao, Muhammad Zohaib Nawaz, Weimin Zhang, Jianxiong Jiang, Jianzhong Sun
Environmental Science and Pollution Research.2022; 29(30): 45793. CrossRef - Spatial patterns and co-occurrence networks of microbial communities related to environmental heterogeneity in deep-sea surface sediments around Yap Trench, Western Pacific Ocean
Chenru Zhang, Qian Liu, Xianrong Li, Min Wang, Xiaoshou Liu, Jinpeng Yang, Jishang Xu, Yong Jiang
Science of The Total Environment.2021; 759: 143799. CrossRef - Bacterial and Fungal Diversity in Sediment and Water Column From the Abyssal Regions of the Indian Ocean
Natasha Maria Barnes, Samir R. Damare, Belle Damodara Shenoy
Frontiers in Marine Science.2021;[Epub] CrossRef - High-throughput single-cell cultivation reveals the underexplored rare biosphere in deep-sea sediments along the Southwest Indian Ridge
Beiyu Hu, Bingxue Xu, Juanli Yun, Jian Wang, Bingliang Xie, Caiming Li, Yanghuan Yu, Ying Lan, Yaxin Zhu, Xin Dai, Ying Huang, Li Huang, Jianzhang Pan, Wenbin Du
Lab on a Chip.2020; 20(2): 363. CrossRef - Polyphasic approach revealed complex bacterial community structure and function in deep sea sediment of ultra-slow spreading Southwest Indian Ridge
Rupesh Kumar Sinha, K.P. Krishnan, Femi Anna Thomas, M.B. Binish, Mahesh Mohan, P. John Kurian
Ecological Indicators.2019; 96: 40. CrossRef - Macrofaunal burrowing enhances deep-sea carbonate lithification on the Southwest Indian Ridge
Hengchao Xu, Xiaotong Peng, Shun Chen, Jiwei Li, Shamik Dasgupta, Kaiwen Ta, Mengran Du
Biogeosciences.2018; 15(21): 6387. CrossRef - Lipid Biomarkers Reveal Microbial Communities in Hydrothermal Chimney Structures from the 49.6°E Hydrothermal Vent Field at the Southwest Indian Ocean Ridge
Jijiang Lei, Fengyou Chu, Xiaoguo Yu, Xiaohu Li, Chunhui Tao
Geomicrobiology Journal.2017; 34(6): 557. CrossRef - Fungal communities from the calcareous deep-sea sediments in the Southwest India Ridge revealed by Illumina sequencing technology
Likui Zhang, Manyu Kang, Yangchao Huang, Lixiang Yang
World Journal of Microbiology and Biotechnology.2016;[Epub] CrossRef - Bacterial and archaeal communities in the deep-sea sediments of inactive hydrothermal vents in the Southwest India Ridge
Likui Zhang, Manyu Kang, Jiajun Xu, Jian Xu, Yinjie Shuai, Xiaojian Zhou, Zhihui Yang, Kesen Ma
Scientific Reports.2016;[Epub] CrossRef - Diversity, Biogeography, and Biodegradation Potential of Actinobacteria in the Deep-Sea Sediments along the Southwest Indian Ridge
Ping Chen, Limin Zhang, Xiaoxuan Guo, Xin Dai, Li Liu, Lijun Xi, Jian Wang, Lei Song, Yuezhu Wang, Yaxin Zhu, Li Huang, Ying Huang
Frontiers in Microbiology.2016;[Epub] CrossRef
- Effect of Fumarate Reducing Bacteria on In Vitro Rumen Fermentation, Methane Mitigation and Microbial Diversity
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Lovelia Mamuad , Seon Ho Kim , Chang Dae Jeong , Yeon Jae Choi , Che Ok Jeon , Sang-Suk Lee
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J. Microbiol. 2014;52(2):120-128. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3518-1
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Abstract
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The metabolic pathways involved in hydrogen (H2) production, utilization and the activity of methanogens are the important factors that should be considered in controlling methane (CH4) emissions by ruminants. H2 as one of the major substrate for CH4 production is therefore should be controlled. One of the strategies on reducing CH4 is through the use of hydrogenotrophic microorganisms such as fumarate reducing bacteria. This study determined the effect of fumarate reducing bacteria, Mitsuokella jalaludinii, supplementation on in vitro rumen fermentation, CH4 production, diversity and quantity. M. jalaludinii significantly reduced CH4 at 48 and 72 h of incubation and significantly increased succinate at 24 h. Although not significantly different, propionate was found to be highest in treatment containing M. jalaludinii at 12 and 48 h of incubation. These results suggest that supplementation of fumarate reducing bacteria to ruminal fermentation reduces CH4 production and quantity, increases succinate and changes the rumen microbial diversity.
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Journal of Fungi.2022; 8(4): 338. CrossRef - Dietary wheat and reduced methane yield are linked to rumen microbiome changes in dairy cows
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PLOS ONE.2022; 17(5): e0268157. CrossRef - Effect of Autochthonous Nepalese Fruits on Nutrient Degradation, Fermentation Kinetics, Total Gas Production, and Methane Production in In-Vitro Rumen Fermentation
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Livestock Science.2020; 231: 103862. CrossRef - Metabolic Hydrogen Flows in Rumen Fermentation: Principles and Possibilities of Interventions
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Frontiers in Microbiology.2020;[Epub] CrossRef - Rumen fermentation and microbial community composition influenced by live Enterococcus faecium supplementation
Lovelia L. Mamuad, Seon Ho Kim, Ashraf A. Biswas, Zhongtang Yu, Kwang-Keun Cho, Sang-Bum Kim, Kichoon Lee, Sang Suk Lee
AMB Express.2019;[Epub] CrossRef - Advanced estimation and mitigation strategies: a cumulative approach to enteric methane abatement from ruminants
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Lovelia L. Mamuad, Sung Sill Lee, Sang Suk Lee
Asian-Australasian Journal of Animal Sciences.2019; 32(8): 1321. CrossRef - Effects of illite supplementation on in vitro and in vivo rumen fermentation, microbial population and methane emission of Hanwoo steers fed high concentrate diets
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Animal Science Journal.2018; 89(1): 114. CrossRef - Effect of different concentrate diet levels on rumen fluid inoculum used for determination of in vitro rumen fermentation, methane concentration, and methanogen abundance and diversity
Seon-Ho Kim, Lovelia L. Mamuad, Eun-Joong Kim, Ha-Guyn Sung, Gui-Seck Bae, Kwang-Keun Cho, Chanhee Lee, Sang-Suk Lee
Italian Journal of Animal Science.2018; 17(2): 359. CrossRef - Methanobacterium formicicum as a target rumen methanogen for the development of new methane mitigation interventions: A review
P Chellapandi, M Bharathi, C Sangavai, R Prathiviraj
Veterinary and Animal Science.2018; 6: 86. CrossRef - Rumen prokaryotic communities of ruminants under different feeding paradigms on the Qinghai-Tibetan Plateau
Dan Xue, Huai Chen, Xinquan Zhao, Shixiao Xu, Linyong Hu, Tianwei Xu, Lin Jiang, Wei Zhan
Systematic and Applied Microbiology.2017; 40(4): 227. CrossRef - Increased propionate concentration inLactobacillus mucosae-fermented wet brewers grains and duringin vitrorumen fermentation
L.L. Mamuad, S.H. Kim, Y.J. Choi, A.P. Soriano, K.K. Cho, K. Lee, G.S. Bae, S.S. Lee
Journal of Applied Microbiology.2017; 123(1): 29. CrossRef - Use of Lysozyme as a Feed Additive on In vitro Rumen Fermentation and Methane Emission
Ashraf A. Biswas, Sung Sill Lee, Lovelia L. Mamuad, Seon-Ho Kim, Yeon-Jae Choi, Gui-Seck Bae, Kichoon Lee, Ha-Guyn Sung, Sang-Suk Lee
Asian-Australasian Journal of Animal Sciences.2016; 29(11): 1601. CrossRef - Quantification of organic acids in ruminal in vitro batch culture fermentation supplemented with fumarate using a herb mix as a substrate
J. Pisarčíková, Z. Váradyová, K. Mihaliková, S. Kišidayová, J. Plaizier
Canadian Journal of Animal Science.2016; 96(1): 60. CrossRef - Rumen fermentation and performance of Hanwoo steers fed total mixed ration with Korean rice wine residue
Chang-Dae Jeong, Lovelia L. Mamuad, Jong Youl Ko, Ha Guyn Sung, Keun Kyu Park, Yoo Kyung Lee, Sang-Suk Lee
Journal of Animal Science and Technology.2016;[Epub] CrossRef - Limits to Dihydrogen Incorporation into Electron Sinks Alternative to Methanogenesis in Ruminal Fermentation
Emilio M. Ungerfeld
Frontiers in Microbiology.2015;[Epub] CrossRef - Effect of Soybean Meal and Soluble Starch on Biogenic Amine Production and Microbial Diversity Using In vitro Rumen Fermentation
Chang-Dae Jeong, Lovelia L. Mamuad, Seon-Ho Kim, Yeon Jae Choi, Alvin P. Soriano, Kwang Keun Cho, Che-Ok Jeon, Sung Sil Lee, Sang-Suk Lee
Asian-Australasian Journal of Animal Sciences.2014; 28(1): 50. CrossRef
- Functional Analysis of a Subtilisin-like Serine Protease Gene from Biocontrol Fungus Trichoderma harzianum
-
Haijuan Fan , Zhihua Liu , Rongshu Zhang , Na Wang , Kai Dou , Gulijimila Mijiti , Guiping Diao , Zhiying Wang
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J. Microbiol. 2014;52(2):129-138. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3308-9
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Abstract
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The subtilisin-like serine protease gene ThSS45 has been cloned from Trichoderma harzianum ACCC30371. Its coding region is 1302 bp in length, encoding 433 amino acids, with a predicted protein molecular weight of 44.9 kDa and pI of 5.91. ThSS45 was shown by RT-qPCR analysis to be differentially transcribed in response to eight different treatments. The transcription of ThSS45 was up-regulated when grown in mineral medium, under carbon starvation, and nitrogen starvation, and in the presence of 1% root powder, 1% stem powder, and 1% leaf powder derived from Populus davidiana × P. bolleana (Shanxin poplar) aseptic seedlings. The highest increase in transcription approached 3.5-fold that of the control at 6 h under induction with 1% poplar root powder. The transcription of ThSS45 was also slightly up-regulated by 1% Alternaria alternata cell wall and 5% A. alternata fermentation liquid. Moreover, the analyses of coding and promoter regions of ThSS45 homologs indicated that serine protease may be involved in both mycoparasitism and antibiotic secretion. ThSS45 was cloned into the pGEX-4T-2 vector and then expressed in Escherichia coli BL21. The recombinant protein, with an expected molecular weight of approximately 69 kDa, was then purified. When transformant BL21-ss was induced with 1 mM IPTG for 6 h, the purified protease activity reached a peak of 18.25 U/ml at pH 7.0 and 40°C. In antifungal assays the purified protease obviously inhibited the growth of A. alternata mycelia.
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- The role of Trichoderma koningii and Trichoderma harzianum in mitigating the combined stresses motivated by Sclerotiniasclerotiorum and salinity in common bean (Phaseolusvulgaris)
Abdelrazek S. Abdelrhim, Nada F. Hemeda, Mai Ali Mwaheb, Maha O.A. Omar, Mona F.A. Dawood
Plant Stress.2024; 11: 100370. CrossRef - Mechanism of oxalate decarboxylase Oxd_S12 from Bacillus velezensis BvZ45-1 in defence against cotton verticillium wilt
Ying Sun, Na Yang, Sirui Li, Fei Chen, Yijing Xie, Canming Tang, Monica Höfte
Journal of Experimental Botany.2024; 75(11): 3500. CrossRef - Purification and Identification of the Nematicidal Activity of S1 Family Trypsin-Like Serine Protease (PRA1) from Trichoderma longibrachiatum T6 Through Prokaryotic Expression and Biological Function Assays
Nan Ma, Hang Lv, Solomon Boamah, Shuwu Zhang, Bingliang Xu
Genes.2024; 15(11): 1437. CrossRef - Genome and transcriptome sequencing of Trichoderma harzianum T4, an important biocontrol fungus of Rhizoctonia solani, reveals genes related to mycoparasitism
Yaping Wang, Jian Wang, Xiaochong Zhu, Wei Wang
Canadian Journal of Microbiology.2024; 70(3): 86. CrossRef - Strain improvement of Trichoderma harzianum for enhanced biocontrol capacity: Strategies and prospects
Ziyang Xiao, Qinqin Zhao, Wei Li, Liwei Gao, Guodong Liu
Frontiers in Microbiology.2023;[Epub] CrossRef - Analysis of the Properties of 44 ABC Transporter Genes from Biocontrol Agent Trichoderma asperellum ACCC30536 and Their Responses to Pathogenic Alternaria alternata Toxin Stress
Hua-Ying Du, Yu-Zhou Zhang, Kuo Liu, Pei-Wen Gu, Shuang Cao, Xiang Gao, Zhi-Ying Wang, Zhi-Hua Liu, Ze-Yang Yu
Current Issues in Molecular Biology.2023; 45(2): 1570. CrossRef - Insights into the ecological generalist lifestyle of Clonostachys fungi through analysis of their predicted secretomes
Edoardo Piombo, Micol Guaschino, Dan Funck Jensen, Magnus Karlsson, Mukesh Dubey
Frontiers in Microbiology.2023;[Epub] CrossRef - The dark septate endophyte Phialocephala sphaeroides suppresses conifer pathogen transcripts and promotes root growth of Norway spruce
Kai Wang, Zilan Wen, Fred O Asiegbu, Malin Elfstrand
Tree Physiology.2022; 42(12): 2627. CrossRef - Extracellular proteins of Trichoderma and their role in plant health
Anu Sharma, Richa Salwan, Vivek Sharma
South African Journal of Botany.2022; 147: 359. CrossRef - Predicted Input of Uncultured Fungal Symbionts to a Lichen Symbiosis from Metagenome-Assembled Genomes
Gulnara Tagirdzhanova, Paul Saary, Jeffrey P Tingley, David Díaz-Escandón, D Wade Abbott, Robert D Finn, Toby Spribille, Jason Stajich
Genome Biology and Evolution.2021;[Epub] CrossRef - Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis
Ruud Heshof, Bram Visscher, Eric van de Zilver, Rick van de Vondervoort, Femke van Keulen, Roy J. B. M. Delahaije, Richèle D. Wind
AMB Express.2020;[Epub] CrossRef - An approach to select Lactobacillus isolates as protective cultures for food fermentations
Raffael C. Inglin, Alessia I. Delbrück, Benjamin Fässler, Katharina E. Siebenmann, Christophe Lacroix, Marc J. A. Stevens, Leo Meile
Journal of Food Safety.2018;[Epub] CrossRef - Biocontrol activity of recombinant aspartic protease from Trichoderma harzianum against pathogenic fungi
Jun-Jin Deng, Wei-Qian Huang, Zhi-Wei Li, De-Lin Lu, Yuanyuan Zhang, Xiao-chun Luo
Enzyme and Microbial Technology.2018; 112: 35. CrossRef - Functional analysis of eliciting plant response protein Epl1-Tas from Trichoderma asperellum ACCC30536
Wenjing Yu, Gulijimila Mijiti, Ying Huang, Haijuan Fan, Yucheng Wang, Zhihua Liu
Scientific Reports.2018;[Epub] CrossRef - Comparative evolutionary histories of fungal proteases reveal gene gains in the mycoparasitic and nematode-parasitic fungus Clonostachys rosea
Mudassir Iqbal, Mukesh Dubey, Mikael Gudmundsson, Maria Viketoft, Dan Funck Jensen, Magnus Karlsson
BMC Evolutionary Biology.2018;[Epub] CrossRef - Expression analysis on mycoparasitism related genes during antagonism of Trichoderma with Colletotrichum falcatum causing red rot in sugarcane
Elangovan Elamathi, Palaniyandi Malathi, Rasappa Viswanathan, Amalraj Ramesh Sundar
Journal of Plant Biochemistry and Biotechnology.2018; 27(3): 351. CrossRef - Subtilisin-like serine protease gene TghSS42 from Trichoderma ghanense ACCC 30153 was successfully expressed in Escherichia coli and recombinant protease rTghSS42 exhibited antifungal ability to five phytopathogens
HUIFANG ZHANG, NA WANG, YUCHENG WANG, JINJIE WANG, HONG ZHENG, ZHIHUA LIU
Biocontrol Science.2017; 22(3): 145. CrossRef - A novel organic solvent- and detergent-stable serine alkaline protease from Trametes cingulata strain CTM10101
Maroua Omrane Benmrad, Emna Moujehed, Mouna Ben Elhoul, Nadia Zaraî Jaouadi, Sondes Mechri, Hatem Rekik, Sidali Kourdali, Mohamed El Hattab, Abdelmalek Badis, Sami Sayadi, Samir Bejar, Bassem Jaouadi
International Journal of Biological Macromolecules.2016; 91: 961. CrossRef - Differential Response of Extracellular Proteases of Trichoderma Harzianum Against Fungal Phytopathogens
Vivek Sharma, Richa Salwan, Prem N. Sharma
Current Microbiology.2016; 73(3): 419. CrossRef - Fungal proteins and genes associated with biocontrol mechanisms of soil-borne pathogens: a review
Yohann Daguerre, Katarzyna Siegel, Véronique Edel-Hermann, Christian Steinberg
Fungal Biology Reviews.2014; 28(4): 97. CrossRef
- Identification and Characterization of Ectoine Biosynthesis Genes and Heterologous Expression of the ectABC Gene Cluster from Halomonas sp. QHL1, a Moderately Halophilic Bacterium Isolated from Qinghai Lake
-
Derui Zhu , Jian Liu , Rui Han , Guoping Shen , Qifu Long , Xiaoxing Wei , Deli Liu
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J. Microbiol. 2014;52(2):139-147. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3389-5
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16
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Abstract
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The moderately halophilic bacterium Halomonas sp. QHL1 was identified as a member of the genus Halomonas by 16S rRNA gene sequencing. HPLC analysis showed that strain QHL1 synthesizes ectoine in its cytoplasm. The genes involved in the ectoine biosynthesis pathway were identified on the chromosome in the order ectABC. Subsequently, the ectB gene from this strain was amplified by PCR, and the entire ectABC gene cluster (3,580 bp) was cloned using genome walking. Analysis showed that the ectA (579 bp), ectB (1269 bp), and ectC (390 bp) genes were organized in a single transcriptional unit and were predicted to encode three peptides of 21.2 kDa, 46.4 kDa, and 14.7 kDa, respectively. Two putative promoters, a δ70-dependent promoter and a δ38-controlled promoter, as well as several conserved motifs with unknown function were identified. Individual ectA, ectB, and ectC genes, and the entire ectABC gene cluster were inserted into the expression plasmid pET-28a(+) to generate the recombinant plasmids pET-28a(+)-ectA, pET-28a(+)-ectB, pET-28a(+)-ectC and pET-28a(+)-ectABC, respectively. Heterologous expression of these proteins in Escherichia coli BL21 (DE3) was confirmed by SDS-PAGE. The recombinant E. coli strain BL21 (pET-28a (+)-ectABC) displayed a higher salt tolerance than native E. coli cells but produced far less ectoine than the wild-type QHL1 strain.
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- Biotechnological production of ectoine: current status and prospects
Jun Chen, Deliang Qiao, Tao Yuan, Yeyuan Feng, Pengjun Zhang, Xuejun Wang, Li Zhang
Folia Microbiologica.2024; 69(2): 247. CrossRef - Comparative genomic analysis of Halomonas campaniensis wild-type and ultraviolet radiation-mutated strains reveal genomic differences associated with increased ectoine production
Zhibo Wang, Yongzhen Li, Xiang Gao, Jiangwa Xing, Rong Wang, Derui Zhu, Guoping Shen
International Microbiology.2023; 26(4): 1009. CrossRef - Comparative Genome Analysis of a Novel Alkaliphilic Actinobacterial Species Nesterenkonia haasae
Shuang Wang, Lei Sun, Manik Prabhu Narsing Rao, Bao‑zhu Fang, Wen‑jun Li
Polish Journal of Microbiology.2022; 71(3): 453. CrossRef - Whole genome sequencing of the halophilic Halomonas qaidamensis XH36, a novel species strain with high ectoine production
Tiantian Zhang, Tianqi Cui, Yaning Cao, Yongzhen Li, Fenghui Li, Derui Zhu, Jiangwa Xing
Antonie van Leeuwenhoek.2022; 115(4): 545. CrossRef - Identification and characterization of an ectoine biosynthesis gene cluster from Aestuariispira ectoiniformans sp. nov., isolated from seawater
Ji Young Kang, Binna Lee, Jeong Ah Kim, Min-Soo Kim, Chul Ho Kim
Microbiological Research.2022; 254: 126898. CrossRef -
Genome Sequence of
Streptomyces
sp. Strain GQFP Isolated from Soil Near the Roots of Pharmaceutical Plant Elaeagnus pungens
Jie Zhu, David A. Baltrus
Microbiology Resource Announcements.2022;[Epub] CrossRef - Enhanced production of ectoine from methane using metabolically engineered Methylomicrobium alcaliphilum 20Z
Sukhyeong Cho, Yun Seo Lee, Hanyu Chai, Sang Eun Lim, Jeong Geol Na, Jinwon Lee
Biotechnology for Biofuels and Bioproducts.2022;[Epub] CrossRef - Ectoine Production Using Novel Heterologous EctABCS. salarius from Marine Bacterium Salinicola salarius
Yue Su, Wenting Peng, Tong Wang, Yanhui Li, Luyu Zhao, Xinyu Wang, Ying Li, Ling Lin
Applied Sciences.2021; 11(15): 6873. CrossRef - High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium
Qi Zhao, Shannan Li, Peiwen Lv, Simian Sun, Cuiqing Ma, Ping Xu, Haijun Su, Chunyu Yang
Microbial Cell Factories.2019;[Epub] CrossRef - Analysis of Zobellella denitrificans ZD1 draft genome: Genes and gene clusters responsible for high polyhydroxybutyrate (PHB) production from glycerol under saline conditions and its CRISPR-Cas system
Yu-Wei Wu, Shih-Hung Yang, Myung Hwangbo, Kung-Hui Chu, Chih-Horng Kuo
PLOS ONE.2019; 14(9): e0222143. CrossRef - Engineering the Salt-Inducible Ectoine Promoter Region of Halomonas elongata for Protein Expression in a Unique Stabilizing Environment
Lisa Stiller, Erwin Galinski, Elisabeth Witt
Genes.2018; 9(4): 184. CrossRef - New Alpiniamides From Streptomyces sp. IB2014/011-12 Assembled by an Unusual Hybrid Non-ribosomal Peptide Synthetase Trans-AT Polyketide Synthase Enzyme
Constanze Paulus, Yuriy Rebets, Josef Zapp, Christian Rückert, Jörn Kalinowski, Andriy Luzhetskyy
Frontiers in Microbiology.2018;[Epub] CrossRef - Metabolic engineering of Escherichia coli for L-tryptophan production
V. B. Panichkin, V. A. Livshits, I. V. Biryukova, S. V. Mashko
Applied Biochemistry and Microbiology.2016; 52(9): 783. CrossRef -
Draft Genome Sequence of Halomonas elongata Strain K4, an Endophytic Growth-Promoting Bacterium Enhancing Salinity Tolerance
In Planta
Feras F. Lafi, Juan S. Ramirez-Prado, Intikhab Alam, Vladimir B. Bajic, Heribert Hirt, Maged M. Saad
Genome Announcements.2016;[Epub] CrossRef - High production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinant Escherichia coli
Yong-Zhi He, Jiao Gong, Hai-Ying Yu, Yong Tao, Shan Zhang, Zhi-Yang Dong
Microbial Cell Factories.2015;[Epub] CrossRef - Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis
Wei Chen, Shan Zhang, Peixia Jiang, Jun Yao, Yongzhi He, Lincai Chen, Xiwu Gui, Zhiyang Dong, Shuang-Yan Tang
Metabolic Engineering.2015; 30: 149. CrossRef
- Interaction between the α-Barrel Tip of Vibrio vulnificus TolC Homologs and AcrA Implies the Adapter Bridging Model
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Seunghwa Lee , Saemee Song , Minho Lee , Soonhye Hwang , Ji-Sun Kim , Nam-Chul Ha , Kangseok Lee
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J. Microbiol. 2014;52(2):148-153. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3578-2
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46
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10
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Abstract
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The AcrAB-TolC multidrug efflux pump confers resistance to Escherichia coli against many antibiotics and toxic compounds. The TolC protein is an outer membrane factor that participates in the formation of type I secretion systems. The genome of Vibrio vulnificus encodes two proteins homologous to the E. coli TolC, designated TolCV1 and TolCV2. Here, we show that both TolCV1 and TolCV2 partially complement the E. coli TolC function and physically interact with the membrane fusion protein AcrA, a component of the E. coli AcrAB-TolC efflux pump. Using site-directed mutational analyses and an in vivo cross-linking assay, we demonstrated that the α-barrel tip region of TolC homologs plays a critical role in the formation of functional AcrAB-TolC efflux pumps. Our findings suggest the adapter bridging model as a general assembly mechanism for tripartite drug efflux pumps in Gram-negative bacteria.
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- Progress of Antimicrobial Mechanisms of Stilbenoids
Xiancai Li, Yongqing Li, Binghong Xiong, Shengxiang Qiu
Pharmaceutics.2024; 16(5): 663. CrossRef - Membrane Efflux Pumps of Pathogenic Vibrio Species: Role in Antimicrobial Resistance and Virulence
Jerusha Stephen, Manjusha Lekshmi, Parvathi Ammini, Sanath H. Kumar, Manuel F. Varela
Microorganisms.2022; 10(2): 382. CrossRef - TolCV1 Has Multifaceted Roles During Vibrio vulnificus Infection
Yue Gong, Rui Hong Guo, Joon Haeng Rhee, Young Ran Kim
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system
Inseong Jo, Jin-Sik Kim, Yongbin Xu, Jaekyung Hyun, Kangseok Lee, Nam-Chul Ha
Journal of Microbiology.2019; 57(3): 185. CrossRef - NaCl promotes antibiotic resistance by reducing redox states in Vibrio alginolyticus
Jun Yang, Zao‐Hai Zeng, Man‐Jun Yang, Zhi‐Xue Cheng, Xuan‐Xian Peng, Hui Li
Environmental Microbiology.2018; 20(11): 4022. CrossRef - ATP-Binding Cassette Transporter VcaM from Vibrio cholerae is Dependent on the Outer Membrane Factor Family for Its Function
Wen-Jung Lu, Hsuan-Ju Lin, Thamarai Janganan, Cheng-Yi Li, Wei-Chiang Chin, Vassiliy Bavro, Hong-Ting Lin
International Journal of Molecular Sciences.2018; 19(4): 1000. CrossRef - Functional analysis of Vibrio vulnificus RND efflux pumps homologous to Vibrio cholerae VexAB and VexCD, and to Escherichia coli AcrAB
Seunghwa Lee, Ji-Hyun Yeom, Sojin Seo, Minho Lee, Sarang Kim, Jeehyeon Bae, Kangseok Lee, Jihwan Hwang
Journal of Microbiology.2015; 53(4): 256. CrossRef - Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model
Saemee Song, Jin-Sik Kim, Kangseok Lee, Nam-Chul Ha
Journal of Microbiology.2015; 53(6): 355. CrossRef - Interaction Mediated by the Putative Tip Regions of MdsA and MdsC in the Formation of a Salmonella-Specific Tripartite Efflux Pump
Saemee Song, Soonhye Hwang, Seunghwa Lee, Nam-Chul Ha, Kangseok Lee, Eric Cascales
PLoS ONE.2014; 9(6): e100881. CrossRef - Functional Analysis of TolC Homologs in Vibrio vulnificus
Seunghwa Lee, Saemee Song, Kangseok Lee
Current Microbiology.2014; 68(6): 729. CrossRef
- Plasminogen Activator Inhibitor Type 1 Expression Induced by Lipopolysaccharide of Porphyromonas gingivalis in Human Gingival Fibroblast
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Hee Sam Na , Eun J. Lim , So Y. Jeong , Mi H. Ryu , Mi Hee Park , Jin Chung
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J. Microbiol. 2014;52(2):154-160. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3022-7
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50
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9
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Abstract
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In the gingival tissues of patients with periodontitis, inflammatory responses are mediated by a wide variety of genes. In our previous screening study, plasminogen activator inhibitor type 1 (PAI-1) mRNA binding protein expression was increased in gingiva from periodontitis patients. In this study, we further investigated the signaling pathway involved in PAI-1 expression induced by Porphyromonas gingivalis LPS (Pg LPS) in human gingival fibroblasts (HGF). When HGFs were treated with Pg LPS, both PAI-1 mRNA expression and PAI-1 protein were induced in a dose-dependent manner. Pg LPS induced NF-κB activation and the expressions of PAI-1 mRNA and protein were suppressed by pretreating with a NF-κB inhibitor. Pg LPS also induced ERK, p38, and JNK activation, and Pg LPS-induced PAI-1 expression was inhibited by ERK/p38/JNK inhibitor pretreatment. In conclusion, Pg LPS induced PAI-1 expression through NF-κB and MAP kinases activation in HGF.
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- Impaired fibrinolysis in patients with atrial fibrillation and elevated circulating lipopolysaccharide
Marcin Sadowski, Michał Ząbczyk, Anetta Undas
Journal of Thrombosis and Thrombolysis.2024; 57(5): 842. CrossRef - Impact of scaling and root planing on salivary and serum plasminogen activator inhibitor‐1 expression in patients with periodontitis with and without type 2 diabetes mellitus
Sanjeela R. Guru, Suchetha Aghanashini
Journal of Periodontology.2023; 94(1): 20. CrossRef - Porphyromonas gingivalis adopts intricate and unique molecular mechanisms to survive and persist within the host: a critical update
Aditi Chopra, Subraya G. Bhat, Karthik Sivaraman
Journal of Oral Microbiology.2020; 12(1): 1801090. CrossRef - Periodontal Pathogens and Neuropsychiatric Health
Abhishek Wadhawan, Mark A. Reynolds, Hina Makkar, Alison J. Scott, Eileen Potocki, Andrew J. Hoisington, Lisa A. Brenner, Aline Dagdag, Christopher A. Lowry, Yogesh Dwivedi, Teodor T. Postolache
Current Topics in Medicinal Chemistry.2020; 20(15): 1353. CrossRef - MicroRNA-212-5p regulates the inflammatory response of periodontal ligament cells by targeting myeloid differentiation factor 88
Bo Hua, Junbo Xiang, Lin Guo, Dongling Lu
Archives of Oral Biology.2020; 118: 104831. CrossRef - An update on possible pathogenic mechanisms of periodontal pathogens on renal dysfunction
Aditi Chopra, Karthik Sivaraman
Critical Reviews in Microbiology.2019; 45(5-6): 514. CrossRef - Periodontitis induced byPorphyromonas gingivalisdrives periodontal microbiota dysbiosis and insulin resistance via an impaired adaptive immune response
Vincent Blasco-Baque, Lucile Garidou, Céline Pomié, Quentin Escoula, Pascale Loubieres, Sandrine Le Gall-David, Mathieu Lemaitre, Simon Nicolas, Pascale Klopp, Aurélie Waget, Vincent Azalbert, André Colom, Martine Bonnaure-Mallet, Philippe Kemoun, Matteo
Gut.2017; 66(5): 872. CrossRef - Porphyromonas gingivalis: An Overview of Periodontopathic Pathogen below the Gum Line
Kah Yan How, Keang Peng Song, Kok Gan Chan
Frontiers in Microbiology.2016;[Epub] CrossRef - Porphyromonas gingivalis: Major Periodontopathic Pathogen Overview
Jaroslav Mysak, Stepan Podzimek, Pavla Sommerova, Yelena Lyuya-Mi, Jirina Bartova, Tatjana Janatova, Jarmila Prochazkova, Jana Duskova
Journal of Immunology Research.2014; 2014: 1. CrossRef
- Sequential Immunosuppressive Activities of Bacterial Secondary Metabolites from the Entomopahogenic Bacterium Xenorhabdus nematophila
-
Seonghyeon Eom , Youngjin Park , Yonggyun Kim
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J. Microbiol. 2014;52(2):161-168. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3251-9
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Abstract
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The entomopathogenic bacterium Xenorhabdus nematophila secretes at least eight bacterial metabolites that play crucial roles suppressing target insect immune responses by inhibiting eicosanoid biosynthesis. We analyzed sequential changes in bacterial metabolite production during bacterial growth and analyzed their individual immunosuppressive activities against the insect host, Spodoptera exigua. X. nematophila exhibited a typical bacterial growth pattern in both insect host and culture medium, and eight metabolites were secreted at different time points. At the early growth phase (6–12 h), Ac-FGV and PHPP were detected in significant amounts in the culture broth. At this early phase, both Ac-FGV (18 μg/ml) and oxindole (110 μg/ml) levels significantly inhibited phenoloxidase and phospholipase A2 activities in S. exigua hemolymph. At the late growth phase (12–36 h), all eight metabolites were detected at significant levels (10–140 μg/ml) in the culture broth and were sufficient to induce hemocyte toxicity. These results suggest that X. nematophila sequentially produces immunosuppressive metabolites that might sequentially and cooperatively inhibit different steps of insect immune responses.
-
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Najwa Seddiqi Kallali, Abderrahman Ouijja, Khadija Goura, Salah-Eddine Laasli, Jihane Kenfaoui, Youssef Benseddik, Abdelali Blenzar, Abdelmalek Boutaleb Joutei, Moussa El Jarroudi, Fouad Mokrini, Rachid Lahlali
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Su Hyeon Hwang, Ho Am Jang, Maryam Ali Mohammadie Kojour, Keunho Yun, Yong Seok Lee, Yeon Soo Han, Yong Hun Jo
Scientific Reports.2023;[Epub] CrossRef - Assessment of biocontrol potential of different Steinernema species and their bacterial symbionts, Xenorhabdus species against larvae of almond moth, Ephestia cautella (Walker)
Ebubekir Yüksel, Nesrin Ormanoğlu, Mustafa İmren, Ramazan Canhilal
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Andrea Arévalo-Cortés, Ashish Damania, Yurany Granada, Sara Zuluaga, Rojelio Mejia, Omar Triana-Chavez
Viruses.2022; 14(10): 2197. CrossRef - The Entomopathogenic Nematodes H. bacteriophora and S. carpocapsae Inhibit the Activation of proPO System of the Nipa Palm Hispid Octodonta nipae (Coleoptera: Chrysomelidae)
Nafiu Sanda, Bofeng Hou, Youming Hou
Life.2022; 12(7): 1019. CrossRef - Larvicidal activity of Photorhabdus and Xenorhabdus bacteria isolated from insect parasitic nematodes against Aedes aegypti and Aedes albopictus
Chanakan Subkrasae, Jiranun Ardpairin, Abdulhakam Dumidae, Pichamon Janthu, Paramaporn Muangpat, Raxsina Polseela, Sarunporn Tandhavanant, Aunchalee Thanwisai, Apichat Vitta
Acta Tropica.2022; 235: 106668. CrossRef - Entomopathogenic nematodes as potential and effective biocontrol agents against cutworms, Agrotis spp.: present and future scenario
Deepak Kumar, Pritam Kumari, Rubal Kamboj, Anil Kumar, Prakash Banakar, Vinod Kumar
Egyptian Journal of Biological Pest Control.2022;[Epub] CrossRef - Integrated Biological Control Using a Mixture of Two Entomopathogenic Bacteria, Bacillus thuringiensis and Xenorhabdus hominickii, against Spodoptera exigua and Other Congeners
Md Tafim Hossain Hrithik, Youngjin Park, Hyemi Park, Yonggyun Kim
Insects.2022; 13(10): 860. CrossRef - Photorhabdus and Xenorhabdus as Symbiotic Bacteria for Bio-Control Housefly (Musca domestica L.)
Ahmed Mohamed Abd El-Rah, Amr Mohamed Abdelazeem, Hanaa Elbrense, Santiago Vergara-Pi
Pakistan Journal of Biological Sciences.2022; 25(7): 586. CrossRef - Control Potentials of Three Entomopathogenic Bacterial Isolates for the Carob Moth, Ectomyelois ceratoniae (Lepidoptera: Pyralidae) in Pomegranates
Saqer S. Alotaibi, Hadeer Darwish, Sarah Alharthi, Akram Alghamdi, Ahmed Noureldeen, Ahmed M. Fallatah, András Fodor, Amal Al-Barty, Bander Albogami, Alaa Baazeem
Agriculture.2021; 11(12): 1256. CrossRef - Functional interaction of bacterial virulence factors of Xenorhabdus nematophila with a calcium-independent cytosolic PLA2 of Spodoptera exigua
Vatanparast Mohammad, Yonggyun Kim
Journal of Invertebrate Pathology.2020; 169: 107309. CrossRef - Immunosuppressive Activities of Novel PLA2 Inhibitors from Xenorhabdus hominickii, an Entomopathogenic Bacterium
Md. Mahi Imam Mollah, Aman Dekebo, Yonggyun Kim
Insects.2020; 11(8): 505. CrossRef - The great potential of entomopathogenic bacteria Xenorhabdus and Photorhabdus for mosquito control: a review
Wellington Junior da Silva, Harry Luiz Pilz-Júnior, Ralf Heermann, Onilda Santos da Silva
Parasites & Vectors.2020;[Epub] CrossRef - Benzylideneacetone and other phenylethylamide bacterial metabolites induce apoptosis to kill insects
Md. Mahi Imam Mollah, Fatema Yeasmin, Yonggyun Kim
Journal of Asia-Pacific Entomology.2020; 23(2): 449. CrossRef - Partner-specific induction of Spodoptera frugiperda immune genes in response to the entomopathogenic nematobacterial complex Steinernema carpocapsae-Xenorhabdus nematophila
Louise Huot, Audrey Bigourdan, Sylvie Pagès, Jean-Claude Ogier, Pierre-Alain Girard, Nicolas Nègre, Bernard Duvic
Developmental & Comparative Immunology.2020; 108: 103676. CrossRef - The prophenoloxidase system in Drosophila participates in the anti-nematode immune response
Dustin Cooper, Caitlin Wuebbolt, Christa Heryanto, Ioannis Eleftherianos
Molecular Immunology.2019; 109: 88. CrossRef - Variation in pathogenicity of different strains of Xenorhabdus nematophila; Differential immunosuppressive activities and secondary metabolite production
Md. Ariful Hasan, Shabbir Ahmed, Md. Mahi Imam Mollah, Dongwoon Lee, Yonggyun Kim
Journal of Invertebrate Pathology.2019; 166: 107221. CrossRef - Prostaglandins and Other Eicosanoids in Insects: Biosynthesis and Biological Actions
David Stanley, Yonggyun Kim
Frontiers in Physiology.2019;[Epub] CrossRef - Identification of entomopathogenic nematodes and symbiotic bacteria from Nam Nao National Park in Thailand and larvicidal activity of symbiotic bacteria against Aedes aegypti and Aedes albopictus
Temsiri Yooyangket, Paramaporn Muangpat, Raxsina Polseela, Sarunporn Tandhavanant, Aunchalee Thanwisai, Apichat Vitta, Guido Favia
PLOS ONE.2018; 13(4): e0195681. CrossRef - Differential immunosuppression by inhibiting PLA2 affects virulence of Xenorhabdus hominickii and Photorhabdus temperata temperata
Shabbir Ahmed, Yonggyun Kim
Journal of Invertebrate Pathology.2018; 157: 136. CrossRef - Challenging the larvae of Helicoverpa armigera and assessing the immune responses to nematode-bacterium complex
Istkhar, Ashok Kumar Chaubey
Phytoparasitica.2018; 46(1): 75. CrossRef - Refining the Natural Product Repertoire in Entomopathogenic Bacteria
Nicholas Jay Tobias, Yi-Ming Shi, Helge B. Bode
Trends in Microbiology.2018; 26(10): 833. CrossRef - Role of Endosymbionts in Insect–Parasitic Nematode Interactions
Ioannis Eleftherianos, Shruti Yadav, Eric Kenney, Dustin Cooper, Yaprak Ozakman, Jelena Patrnogic
Trends in Parasitology.2018; 34(5): 430. CrossRef - Eicosanoid mediation of immune responses at early bacterial infection stage and its inhibition by Photorhabdus temperata subsp. temperata, an entomopathogenic bacterium
Hyoil Kim, Duyeol Choi, Jihyeon Jung, Yonggyun Kim
Archives of Insect Biochemistry and Physiology.2018;[Epub] CrossRef - Identification and bacterial characteristics of Xenorhabdus hominickii ANU101 from an entomopathogenic nematode, Steinernema monticolum
Youngjin Park, Sangjin Kang, Md. Sadekuzzaman, Hyeonghwan Kim, Jin-Kyo Jung, Yonggyun Kim
Journal of Invertebrate Pathology.2017; 144: 74. CrossRef - The Global Transcription Factor Lrp Is both Essential for and Inhibitory to Xenorhabdus nematophila Insecticidal Activity
Ángel M. Casanova-Torres, Upasana Shokal, Neta Morag, Ioannis Eleftherianos, Heidi Goodrich-Blair, Harold L. Drake
Applied and Environmental Microbiology.2017;[Epub] CrossRef - Effects of an entomopathogen nematode on the immune response of the insect pest red palm weevil: Focus on the host antimicrobial response
Simona Binda-Rossetti, Maristella Mastore, Marina Protasoni, Maurizio F. Brivio
Journal of Invertebrate Pathology.2016; 133: 110. CrossRef - A Mixture ofBacillus thuringiensissubsp.israelensisWithXenorhabdus nematophila-Cultured Broth Enhances Toxicity Against MosquitoesAedes albopictusandCulex pipiens pallens(Diptera: Culicidae)
Youngjin Park, Jin Kyo Jung, Yonggyun Kim
Journal of Economic Entomology.2016; 109(3): 1086. CrossRef - Microbiology of sugar‐rich environments: diversity, ecology and system constraints
Bart Lievens, John E. Hallsworth, Maria I. Pozo, Zouhaier Ben Belgacem, Andrew Stevenson, Kris A. Willems, Hans Jacquemyn
Environmental Microbiology.2015; 17(2): 278. CrossRef - Comparison of Xenorhabdus bovienii bacterial strain genomes reveals diversity in symbiotic functions
Kristen E. Murfin, Amy C. Whooley, Jonathan L. Klassen, Heidi Goodrich-Blair
BMC Genomics.2015;[Epub] CrossRef - Entomopathogenic bacterium, Xenorhabdus nematophila and Photorhabdus luminescens, enhances Bacillus thuringiensis Cry4Ba toxicity against yellow fever mosquito, Aedes aegypti (Diptera: Culicidae)
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Journal of Asia-Pacific Entomology.2015; 18(3): 459. CrossRef - A Technique to Enhance Insecticidal Efficacy Using Bt Cry Toxin Mixture and Eicosanoid Biosynthesis Inhibitor
Seonghyeon Eom, Youngjin Park, Yonggyun Kim
The Korean Journal of Pesticide Science.2015; 19(3): 301. CrossRef - Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasion
Susana Ramos, Ana Custódio, Henrique Silveira
Memórias do Instituto Oswaldo Cruz.2014; 109(5): 668. CrossRef - Integrin β subunit and its RNA interference in immune and developmental processes of the Oriental tobacco budworm, Helicoverpa assulta
Youngjin Park, Seung-Joon Ahn, Heiko Vogel, Yonggyun Kim
Developmental & Comparative Immunology.2014; 47(1): 59. CrossRef - A Technique to Enhance Bacillus thuringiensis Spectrum and Control Efficacy Using Cry Toxin Mixture and Immunosuppressant
Seonghyeon Eom, Youngjin Park, Yonggyun Kim
The Korean Journal of Pesticide Science.2014; 18(3): 181. CrossRef - Effect of Cellular Phospholipase A2Inhibition on Enhancement of Bt Insecticidal Activity
Seonghyeon Eom, Jiyeong Park, Kunwoo Kim, Yonggyun Kim
Korean journal of applied entomology.2014; 53(3): 271. CrossRef
Journal Article
- Predictive Modelling of Lactobacillus casei KN291 Survival in Fermented Soy Beverage
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Zieli , Koło , Goryl Antoni , Ilona Motyl
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J. Microbiol. 2014;52(2):169-178. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3045-0
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Abstract
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The aim of the study was to construct and verify predictive growth and survival models of a potentially probiotic bacteria in fermented soy beverage. The research material included natural soy beverage (Polgrunt, Poland) and the strain of lactic acid bacteria (LAB) – Lactobacillus casei KN291. To construct predictive models for the growth and survival of L. casei KN291 bacteria in the fermented soy beverage we design an experiment which allowed the collection of CFU data. Fermented soy beverage samples were stored at various temperature conditions (5, 10, 15, and 20°C) for 28 days. On the basis of obtained data concerning the survival of L. casei KN291 bacteria in soy beverage at different temperature and time conditions, two non-linear models (r2= 0.68–0.93) and two surface models (r2=0.76–0.79) were constructed; these models described the behaviour of the bacteria in the product to a satisfactory extent. Verification of the surface models was carried out utilizing the validation data - at 7°C during 28 days. It was found that applied models were well fitted and charged with small systematic errors, which is evidenced by accuracy factor - Af, bias factor - Bf and mean squared error - MSE. The constructed microbiological growth and survival models of L. casei KN291 in fermented soy beverage enable the estimation of products shelf life period, which in this case is defined by the requirement for the level of the bacteria to be above 106 CFU/cm3. The constructed models may be useful as a tool for the manufacture of probiotic foods to estimate of their shelf life period.
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Citations
Citations to this article as recorded by

- Modeling of Growth and Organic Acid Kinetics and Evolution of the Protein Profile and Amino Acid Content during Lactiplantibacillus plantarum ITM21B Fermentation in Liquid Sourdough
Mariaelena Di Biase, Yvan Le Marc, Anna Rita Bavaro, Stella Lisa Lonigro, Michela Verni, Florence Postollec, Francesca Valerio
Foods.2022; 11(23): 3942. CrossRef - A Predictive Growth Model for Pro-technological and Probiotic Lacticaseibacillus paracasei Strains Fermenting White Cabbage
Mariaelena Di Biase, Yvan Le Marc, Anna Rita Bavaro, Palmira De Bellis, Stella Lisa Lonigro, Paola Lavermicocca, Florence Postollec, Francesca Valerio
Frontiers in Microbiology.2022;[Epub] CrossRef - Modeling of Listeria monocytogenes survival and quality attributes of sliced mushroom (Agaricus bisporus) subjected to pulsed UV light
Gamze Koçer Alaşalvar, Nene Meltem Keklik
Journal of Food Process Engineering.2021;[Epub] CrossRef - Effect of Probiotic Soy Milk on Serum Levels of Adiponectin, Inflammatory Mediators, Lipid Profile, and Fasting Blood Glucose Among Patients with Type II Diabetes Mellitus
Sadegh Feizollahzadeh, Reza Ghiasvand, Abbas Rezaei, Hossein Khanahmad, Akram sadeghi, Mitra Hariri
Probiotics and Antimicrobial Proteins.2017; 9(1): 41. CrossRef - Recent research process of fermented plant extract: A review
Yanjun Feng, Min Zhang, Arun S. Mujumdar, Zhongxue Gao
Trends in Food Science & Technology.2017; 65: 40. CrossRef - Stability and functionality of synbiotic soy food during shelf-life
Olga Lucía Mondragón-Bernal, José Guilherme Lembi Ferreira Alves, Mariá Andrade Teixeira, Maria Fernanda Perina Ferreira, Francisco Maugeri Filho
Journal of Functional Foods.2017; 35: 134. CrossRef - Modeling the Effect of Inulin, pH and Storage Time on the Viability of Selected Lactobacillus in a Probiotic Fruity Yogurt Drink Using the Monte Carlo Simulation
Parang Nikmaram, Seyed Mohamad Mousavi, Hossein Kiani, Zahra Emamdjomeh, Seyed Hadi Razavi, Zeinab Mousavi
Journal of Food Quality.2016; 39(4): 362. CrossRef - A randomized, double-blind, placebo-controlled, clinical trial on probiotic soy milk and soy milk: effects on epigenetics and oxidative stress in patients with type II diabetes
Mitra Hariri, Rasoul Salehi, Awat Feizi, Maryam Mirlohi, Reza Ghiasvand, Nahal Habibi
Genes & Nutrition.2015;[Epub] CrossRef
Research Support, Non-U.S. Gov't
- NOTE] Effects of Light Intensity on Components and Topographical Structures of Extracellular Polysaccharides from the Cyanobacteria Nostoc sp.
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Hongmei Ge , Ling Xia , Xuping Zhou , Delu Zhang , Chunxiang Hu
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J. Microbiol. 2014;52(2):179-183. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-2720-5
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Abstract
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A study on the effects of light intensity (40 and 80 μE/m2/sec) on the components and topographical structures of extracellular polysaccharides (EPS) was carried out in cyanobacteria Nostoc sp.. EPS yield increased with light intensity. However, light intensity did not significantly affect the EPS fractions and monosaccharide composition. Higher light intensity generally resulted in higher protein content of EPS in similar fractions. The topographical structure of EPS, investigated by atomic force microscopy, appeared as spherical lumps, chains and networks. The long chains were observed at higher light intensity. Thus, light intensity affected the yield and nature of EPS.
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Citations
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- Bacterial extracellular biopolymers: Eco-diversification, biosynthesis, technological development and commercial applications
Saumi Pandey, Vinod K. Kannaujiya
International Journal of Biological Macromolecules.2024; 279: 135261. CrossRef - A novel bioactive and functional exopolysaccharide from the cyanobacterial strain Arthrospira maxima cultivated under salinity stress
Amel Harbaoui, Nadia Khelifi, Neyssene Aissaoui, Murielle Muzard, Agathe Martinez, Issam Smaali
Bioprocess and Biosystems Engineering.2024;[Epub] CrossRef - Eco-friendly cultivation of microalgae using a horizontal twin layer system for treatment of real solid waste leachate
Sahar Saleem, Zeshan Sheikh, Rashid Iftikhar, Mazhar Iqbal Zafar
Journal of Environmental Management.2024; 351: 119847. CrossRef - Microalgal extracellular polymeric substances (EPS) and their roles in cultivation, biomass harvesting, and bioproducts extraction
Yun Zhou, Xiaocai Cui, Beibei Wu, Ziqi Wang, Ying Liu, Tian Ren, Siqing Xia, Bruce E. Rittmann
Bioresource Technology.2024; 406: 131054. CrossRef - Regulation of ARGs abundance by biofilm colonization on microplastics under selective pressure of antibiotics in river water environment
Zhijie Zheng, Xiaolong Wang, Wenzhu Zhang, Lan Wang, Honghong Lyu, Jingchun Tang
Journal of Environmental Management.2024; 355: 120402. CrossRef - Cyanobacterial and microalgae polymers: antiviral activity and applications
Mariana Barbalho Farias da Silva, Cláudia Maria Luz Lapa Teixeira
Brazilian Journal of Microbiology.2024;[Epub] CrossRef - Microalgal biofilms: Towards a sustainable biomass production
Houda Ennaceri, Tasneema Ishika, Victor Okorie Mkpuma, Navid Reza Moheimani
Algal Research.2023; 72: 103124. CrossRef - Biological activities of polysaccharides fraction from Arthrospira platensis and Nostoc muscorum cultivated under abiotic stress conditions
Walaa S. Abd El Monsef, Awad A. Ragab, Sanaa M. Shanab, Ghada I. Mahmoud, Emad A. Shalaby
Egyptian Pharmaceutical Journal.2023; 22(4): 582. CrossRef - Insight into the formation mechanism of algal biofilm in soy sauce wastewater
Jun Qian, Ting Wan, Yuxin Ye, Jingjing Li, Tatsuki Toda, Hongwu Li, Mutsumi Sekine, Yoshiki Takayama, Shinichi Koga, Shengxi Shao, Liangliang Fan, Peilun Xu, Wenguang Zhou
Journal of Cleaner Production.2023; 394: 136179. CrossRef - Microalgae and cyanobacteria polysaccharides: Important link for nutrient recycling and revalorization of agro-industrial wastewater
Mariana Franco-Morgado, Genaro G. Amador-Espejo, Marisol Pérez-Cortés, Janet Alejandra Gutiérrez-Uribe
Applied Food Research.2023; 3(1): 100296. CrossRef - Performance of a biocrust cyanobacteria-indigenous bacteria (BCIB) co-culture system for nutrient capture and transfer in municipal wastewater
Li Wu, Linghui Quan, Zhikang Deng, Ashiwin Vadiveloo, Yongtao Cheng, Lie Yang, Zulin Zhang, Abdullah A. Saber, Shubin Lan
Science of The Total Environment.2023; 888: 164236. CrossRef - Assessment of optimal growth conditions for biomass and exopolysaccharides production in the thermotolerant cyanobacterium Phormidium sp. ETS-05
Raffaella Margherita Zampieri, Fabrizio Caldara, Nicoletta La Rocca
Journal of Applied Phycology.2023; 35(4): 1575. CrossRef - Anticoagulant and antibacterial activities of polysaccharides fraction from Nostoc muscorum and Arthrospira platensis cultivated under abiotic stress conditions
Walaa S. Abd El Monsef, Awad A. Ragab, Sanaa M. Shanab, Ghada I. Mahmoud, Emad A. Shalaby
Egyptian Pharmaceutical Journal.2023; 22(3): 516. CrossRef - Exopolysaccharides from microalgae: Production in a biorefinery framework and potential applications
M.G. Morais, T.D. Santos, L. Moraes, B.S. Vaz, E.G. Morais, J.A.V. Costa
Bioresource Technology Reports.2022; 18: 101006. CrossRef - Influence of Process Operation on the Production of Exopolysaccharides in Arthrospira platensis and Chlamydomonas asymmetrica
Sun-Hwa Jung, Niklas Zell, Fabian Boßle, Ulrich Teipel, Cornelia Rauh, Christopher McHardy, Christoph Lindenberger
Frontiers in Sustainable Food Systems.2022;[Epub] CrossRef - Promising Biomolecules with High Antioxidant Capacity Derived from Cryptophyte Algae Grown under Different Light Conditions
Maryam Abidizadegan, Jaanika Blomster, David Fewer, Elina Peltomaa
Biology.2022; 11(8): 1112. CrossRef - Exopolysaccharides from Microalgae and Cyanobacteria: Diversity of Strains, Production Strategies, and Applications
Céline Laroche
Marine Drugs.2022; 20(5): 336. CrossRef - The involvement of type IV pili and the phytochrome CphA in gliding motility, lateral motility and photophobotaxis of the cyanobacterium Phormidium lacuna
Tilman Lamparter, Jennifer Babian, Katrin Fröhlich, Marion Mielke, Nora Weber, Nadja Wunsch, Finn Zais, Kevin Schulz, Vera Aschmann, Nina Spohrer, Norbert Krauß, Pushkar P Lele
PLOS ONE.2022; 17(1): e0249509. CrossRef - Nitrogen concentration acting as an environmental signal regulates cyanobacterial EPS excretion
Long Qian, Xingwang Ye, Jingshang Xiao, Simeng Lin, Hongyu Wang, Zhe Liu, Yongfei Ma, Lie Yang, Zulin Zhang, Li Wu
Chemosphere.2022; 291: 132878. CrossRef - Light intensity stimulates the production of extracellular polymeric substances (EPS) in a culture of the desert cyanobacterium Trichormus sp
Pablo Dahech, Michael Schlömann, Claudia Ortiz
Journal of Applied Phycology.2021; 33(5): 2795. CrossRef - Extracellular Polymeric Substances (EPS) as Microalgal Bioproducts: A Review of Factors Affecting EPS Synthesis and Application in Flocculation Processes
Wioleta Babiak, Izabela Krzemińska
Energies.2021; 14(13): 4007. CrossRef - Physiology of microalgal biofilm: a review on prediction of adhesion on substrates
Yi Tong Cheah, Derek Juinn Chieh Chan
Bioengineered.2021; 12(1): 7577. CrossRef - Biocrust Research in China: Recent Progress and Application in Land Degradation Control
Xinrong Li, Rong Hui, Huijuan Tan, Yang Zhao, Rentao Liu, Naiping Song
Frontiers in Plant Science.2021;[Epub] CrossRef - Microalgae Polysaccharides: An Overview of Production, Characterization, and Potential Applications
Jorge Alberto Vieira Costa, Bárbara Franco Lucas, Ana Gabrielle Pires Alvarenga, Juliana Botelho Moreira, Michele Greque de Morais
Polysaccharides.2021; 2(4): 759. CrossRef - New insights into external layers of cyanobacteria and microalgae based on multiscale analysis of AFM force-distance curves
Yan Xiao, Yuran Cheng, Pan He, Xinghua Wu, Zhe Li
Science of The Total Environment.2021; 774: 145680. CrossRef - Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties
Rita Mota, Ricardo Vidal, Carolina Pandeirada, Carlos Flores, Alessandra Adessi, Roberto De Philippis, Cláudia Nunes, Manuel A. Coimbra, Paula Tamagnini
Carbohydrate Polymers.2020; 229: 115525. CrossRef - Modifying the Cyanobacterial Metabolism as a Key to Efficient Biopolymer Production in Photosynthetic Microorganisms
Maciej Ciebiada, Katarzyna Kubiak, Maurycy Daroch
International Journal of Molecular Sciences.2020; 21(19): 7204. CrossRef - Extraction methods of different morphotypes of surface coat in cyanobacteria
LIU Lingxin, XIAO Yan, LI Zhe, WU Xinghua, ZHANG Yu
Journal of Lake Sciences.2020; 32(3): 887. CrossRef - Resilience and self-regulation processes of microalgae under UV radiation stress
Rajesh P. Rastogi, Datta Madamwar, Hitoshi Nakamoto, Aran Incharoensakdi
Journal of Photochemistry and Photobiology C: Photochemistry Reviews.2020; 43: 100322. CrossRef - Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development
Diogo Cruz, Vitor Vasconcelos, Guillaume Pierre, Philippe Michaud, Cédric Delattre
Applied Sciences.2020; 10(11): 3763. CrossRef - Differences in Production, Composition, and Antioxidant Activities of Exopolymeric Substances (EPS) Obtained from Cultures of Endophytic Fusarium culmorum Strains with Different Effects on Cereals
Jolanta Jaroszuk-Ściseł, Artur Nowak, Iwona Komaniecka, Adam Choma, Anna Jarosz-Wilkołazka, Monika Osińska-Jaroszuk, Renata Tyśkiewicz, Adrian Wiater, Jerzy Rogalski
Molecules.2020; 25(3): 616. CrossRef - Characterization and comparison of Porphyridium sordidum and Porphyridium purpureum concerning growth characteristics and polysaccharide production
Edilberto Vicente Medina-Cabrera, Broder Rühmann, Jochen Schmid, Volker Sieber
Algal Research.2020; 49: 101931. CrossRef - Screening of marine microalgae: Investigation of new exopolysaccharide producers
C. Gaignard, C. Laroche, G. Pierre, P. Dubessay, C. Delattre, C. Gardarin, P. Gourvil, I. Probert, A. Dubuffet, P. Michaud
Algal Research.2019; 44: 101711. CrossRef - Enhancing the auto-flocculation of photosynthetic bacteria to realize biomass recovery in brewery wastewater treatment
Haifeng Lu, Shan Dong, Guangming Zhang, Ting Han, Yuanhui Zhang, Baoming Li
Environmental Technology.2019; 40(16): 2147. CrossRef - The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions
Pei-pei Han, Shun-yu Yao, Rong-jun Guo, Shi-gang Shen, Rong-rong Yan, Zhi-lei Tan, Shi-ru Jia
Carbohydrate Polymers.2017; 174: 111. CrossRef - Microalgal-bacterial aggregates: Applications and perspectives for wastewater treatment
Guillermo Quijano, Juan S. Arcila, Germán Buitrón
Biotechnology Advances.2017; 35(6): 772. CrossRef - Low-melanin containing pullulan production from sugarcane bagasse hydrolysate by Aureobasidium pullulans in fermentations assisted by light-emitting diode
Ruly Terán Hilares, Camila Ayres Orsi, Muhammad Ajaz Ahmed, Paulo Franco Marcelino, Carlos Renato Menegatti, Silvio Silvério da Silva, Júlio César dos Santos
Bioresource Technology.2017; 230: 76. CrossRef - The effects of the exopolysaccharide and growth rate on the morphogenesis of the terrestrial filamentous cyanobacterium Nostoc flagelliforme
Lijuan Cui, Haiyan Xu, Zhaoxia Zhu, Xiang Gao
Biology Open.2017; 6(9): 1329. CrossRef - Influence of solar irradiance levels on the formation of microalgae-bacteria aggregates for municipal wastewater treatment
Juan S. Arcila, Germán Buitrón
Algal Research.2017; 27: 190. CrossRef - Production, extraction and characterization of microalgal and cyanobacterial exopolysaccharides
Cédric Delattre, Guillaume Pierre, Céline Laroche, Philippe Michaud
Biotechnology Advances.2016; 34(7): 1159. CrossRef - Potentials of the microalgae inoculant in restoration of biological soil crusts to combat desertification
A. Lababpour
International Journal of Environmental Science and Technology.2016; 13(10): 2521. CrossRef - High-resolution imaging of the microbial cell surface
Ki Woo Kim
Journal of Microbiology.2016; 54(11): 703. CrossRef
- Erratum] Inhibitory Effect of Chlorophyllin on the Propionibacterium acnes-Induced Chemokine Expression
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Mi-Sun Kang , Jin-Hee Kim , Boo-Ahn Shin , Hyun-Chul Lee , Youn-Shin Kim , Hae-Soon Lim , Jong-Suk Oh
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J. Microbiol. 2014;52(2):184-184.
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DOI: https://doi.org/10.1007/s12275-014-0698-7
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Abstract
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In the article by Kang et al. published in Journal of Microbiology 2013; 51, 844-849. Figure 4 on page 847 should be changed as below.