Journal of Microbiology

Volume 47(5); October 2009

Research Support, Non-U.S. Gov'ts

Antarcticimonas flava gen. nov., sp. nov., Isolated from Antarctic Coastal Seawater: Seung-Jo Yang , Hyun-Myung Oh , Sangyun Chung , Jang-Cheon Cho; J. Microbiol. 2009;47(5):517-523. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0225-4

80 View
0 Download
9 Scopus

Abstract: A marine bacterium, designated IMCC3175T, was isolated from a seawater sample collected off the Antarctic coast. The strain was Gram-negative, obligately aerobic, carotenoid pigment-containing, and rod-shaped bacterium that divided by binary fission. As determined by 16S rRNA gene sequence comparisons, the most closely related genera were Formosa (92.9~93.3%), Bizionia (91.6~93.2%), Gaetbulibacter (91.5~92.8%), Sediminibacter (92.7%), Yeosuana (92.6%), Subsaximicrobium (92.1~92.2%), and Gillisia (89.5~92.2%). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a monophyletic clade together with the genera Sediminibacter and Subsaximicrobium but represented an independent phyletic line in this clade of the family Flavobacteriaceae. The DNA G+C content of the strain was 37.3 mol%. The major respiratory quinone was MK-6 and the predominant cellular fatty acids were C16:1 ω7c and/or iso-C15:0 2-OH (12.8%), anteiso-C15:0 (9.4%), and iso-C16:1 (9.4%). Low 16S rRNA gene sequence similarity, formation of a distinct phylogenetic branch, and several phenotypic characteristics, including a narrow range of temperature and salinity for growth, differentiated strain IMCC3175T from other related genera in the family Flavobacteriaceae. Therefore the name Antarcticimonas flava gen. nov., sp. nov. is proposed, with strain IMCC3175T (=KCCM 42713T =NBRC 103398T) as the type strain.

Paenibacillus filicis sp. nov., Isolated from the Rhizosphere of the Fern: Byung-Chun Kim , Mi Na Kim , Kang Hyun Lee , Sun Beom Kwon , Kyung Sook Bae , Kee-Sun Shin; J. Microbiol. 2009;47(5):524-529. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0266-8

112 View
0 Download
8 Crossref

A Gram-positive and endospore-forming bacterial strain, designated S4T, was isolated from the rhizosphere of ferns in Daejeon, Republic of Korea. This isolate is strictly aerobic, motile, and rod-like in shape, and it is positive for catalase, oxidase, esterase lipase, and β-galactosidase activities. In addition, this strain grows when cultured at temperatures between 15 and 37°C and at pH values ranging from 5.5 to 9.0. The DNA G+C content was determined to be 53.2 mol%. Strain S4T has meso-diaminopimelic acid in the cell-wall peptidoglycan; it also contains menaquinone 7 (MK-7) as the predominant isoprenoid quinone and anteiso-C15:0 (57.5%), iso-C16:0 (11.3%), and C16:0 (9.4%) as the major cellular fatty acids. Phylogenetic analysis based on alignments of the 16S rRNA gene sequence showed that S4T is affiliated with a cluster of strains within the genus Paenibacillaceae and is most closely related to Paenibacillus chinjuensis WN9T, with 96.8% similarity. Based on the phylogenetic and phenotypic characteristics of strain S4T, we believe that this isolate should be distinguished from all type species of the genus Paenibacillus and should thus represent a novel taxon within the genus Paenibacillus. We propose naming this type species Paenibacillus filicis sp. nov. for the rhizosphere isolate; the type strain will be known as S4T (=KCTC 13693T =KACC 14197T =JCM 16417T).

Citations

Citations to this article as recorded by

Paenibacillus gyeongsangnamensis sp. nov., Isolated from Soil
Hyosun Lee, Dhiraj Kumar Chaudhary, Dong-Uk Kim
Journal of Microbiology and Biotechnology.2024; 34(8): 1636. CrossRef
Paenibacillus puerhi sp. nov., isolated from the rhizosphere soil of Pu-erh tea plants (Camellia sinensis var. assamica)
Rui-Juan Yang, De Zhou, Qiao-Mei Wang, Xing-Hua Wang, Wen-Jie Zhang, Li Zhuang, Xuan-Jun Wang, Liang Yan, Jie Lv, Jun Sheng
Archives of Microbiology.2021; 203(4): 1375. CrossRef
Paenibacillus konkukensis sp. nov., isolated from animal feed
Wan-Taek Im, Kwon-Jung Yi, Sang-Suk Lee, Hyung In Moon, Che Ok Jeon, Dong-Woon Kim, Soo-Ki Kim
International Journal of Systematic and Evolutionary Microbiology.2017; 67(7): 2343. CrossRef
Paenibacillus cucumis sp. nov. isolated from greenhouse soil
Jae-Hyung Ahn, Byoung-chan Kim, Byung-Yong Kim, Soo-Jin Kim, Jaekyeong Song, Soon-Wo Kwon, Hang-Yeon Weon
Journal of Microbiology.2014; 52(6): 460. CrossRef
Paenibacillus vulneris sp. nov., isolated from a necrotic wound
Stefanie P. Glaeser, Enevold Falsen, Hans-Jürgen Busse, Peter Kämpfer
International Journal of Systematic and Evolutionary Microbiology .2013; 63(Pt_2): 777. CrossRef
Identification and Characterization of Psychrotolerant Sporeformers Associated with Fluid Milk Production and Processing
Reid A. Ivy, Matthew L. Ranieri, Nicole H. Martin, Henk C. den Bakker, Bruno M. Xavier, Martin Wiedmann, Kathryn J. Boor
Applied and Environmental Microbiology.2012; 78(6): 1853. CrossRef
List of new names and new combinations previously effectively, but not validly, published

International Journal of Systematic and Evolutionary Microbiology .2010; 60(1): 1. CrossRef
Paenibacillus pini sp. nov., a cellulolytic bacterium isolated from the rhizosphere of pine tree
Byung-Chun Kim, Kang Hyun Lee, Mi Na Kim, Eun-Mi Kim, Sung Ran Min, Hyun Soon Kim, Kee-Sun Shin
The Journal of Microbiology.2009; 47(6): 699. CrossRef

Paenibacillus pinihumi sp. nov., a Cellulolytic Bacterium Isolated from the Rhizosphere of Pinus densiflora: Byung-Chun Kim , Kang Hyun Lee , Mi Na Kim , Eun-Mi Kim , Moon-Soo Rhee , O-Yu Kwon , Kee-Sun Shin; J. Microbiol. 2009;47(5):530-535. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0270-z

85 View
0 Download
22 Scopus

Abstract: A novel cellulolytic bacterium, strain S23T, was isolated from the rhizosphere of the pine trees in Daejeon, Republic of Korea. This isolate was Gram-positive, strictly aerobic, rod-shaped, catalase-negative, oxidase- positive, motile by means of peritrichous flagella, and tested positive for alkaline phosphatase, esterase lipase, leucine arylamidase, α-galactosidase, and β-galactosidase activities. The DNA G+C content was 49.5mol%. The main cellular fatty acids were anteiso-C15:0 (51.9%), iso-C16:0 (14.7%), and iso-C15:0 (13.2%). The major isoprenoid quinone was menaquinone 7 (MK-7). Diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. Comparative 16S rRNA gene sequence analysis showed that this strain clustered with Paenibacillus species. The 16S rRNA gene sequence similarity values between S23T and other Paenibacillus species were between 89.9% and 95.9%, and S23T was most closely related to Paenibacillus tarimensis SA-7-6T. On the basis of phylogenetic and phenotypic properties of strain S23T, the isolate is considered as a novel species belonging to the genus Paenibacillus. Therefore, the name, Paenibacillus pinihumi sp. nov., is proposed for the rhizosphere isolate; the type strain is S23T (=KCTC 13695T =KACC 14199T =JCM 16419T)

Nitroreductase II Involved in 2,4,6-Trinitrotoluene Degradation: Purification and Characterization from Klebsiella sp. C1: Jung-Hye Shin , Hong-Gyu Song; J. Microbiol. 2009;47(5):536-541. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-008-0171-6

93 View
0 Download
14 Scopus

Abstract: Three 2,4,6-trinitrotoluene (TNT) nitroreductases from Klebsiella sp. C1 have different reduction capabilities that can degrade TNT by simultaneous utilization of two initial reduction pathways. Of these, nitroreductase II was purified to homogeneity by sequential chromatographies. Nitroreductase II is an oxygen- insensitive enzyme and reduces both TNT and nitroblue tetrazolium. The N-terminal amino acid sequence of the enzyme did not show any sequence similarity with those of other nitroreductases reported. However, it transformed TNT by the reduction of nitro groups like nitroreductase I. It had a higher substrate affinity and specific activity for TNT reduction than other nitroreductases, and it showed a higher oxidation rate of NADPH with the ortho-substituted isomers of TNT metabolites (2-hydroxylaminodinitrotoluene and 2-aminodinitrotoluene) than with para-substituted compounds (4-hydroxylaminodinitrotoluene and 4-aminodinitrotoluene).

Characterization of a Novel β-Glucosidase-Like Activity from a Soil Metagenome: Chengjian Jiang , Gefei Ma , Shuangxi Li , Tingting Hu , Zhiqun Che , Peihong Shen , Bing Yan , Bo Wu; J. Microbiol. 2009;47(5):542-548. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0024-y

90 View
0 Download
29 Crossref

Abstract

We report the cloning of a novel β-glucosidase-like gene by function-based screening of a metagenomic library from uncultured soil microorganisms. The gene was named bgl1C and has an open reading frame of 1,443 base pairs. It encodes a 481 amino acid polypeptide with a predicted molecular mass of about 57.8 kDa. The deduced amino acid sequence did not show any homology with known β-glucosidases. The putative β-glucosidase gene was subcloned into the pETBlue-2 vector and overexpressed in E. coli Tuner (DE3) pLacІ; the recombinant protein was purified to homogeneity. Functional characterization with a high performance liquid chromatography method demonstrated that the recombinant Bgl1C protein hydrolyzed D-glucosyl-β-(1-4)-D-glucose to glucose. The maximum activity for Bgl1C protein occurred at pH 8.0 and 42°C using p-nitrophenyl-β-D-glucoside as the substrate. A CaCl2 concentration of 1 mM was required for optimal activity. The putative β-glucosidase had an apparent Km value of 0.19 mM, a Vmax value of 4.75 U/mg and a kcat value of 316.7/min under the optimal reaction conditions. The biochemical characterization of Bgl1C has enlarged our understanding of the novel enzymes that can be isolated from the soil metagenome.

Citations

Citations to this article as recorded by

Nature-Inspired Bioreactor for Enzymatic Hydrolysis of Non-Newtonian Biomass Slurry: A Numerical Study
Ao Xia, Chang Zhang, Tong Zhu, Kai Lin, Du Zhang, Yun Huang, Xianqing Zhu, Xun Zhu, Qiang Liao
Industrial & Engineering Chemistry Research.2025; 64(5): 3004. CrossRef
Insight into the synergistic effects of surface microstructures and wall peristalsis on glucose production in a bionic microreactor
Kai Lin, Ao Xia, Yun Huang, Xianqing Zhu, Xun Zhu, Kaiyong Cai, Zidong Wei, Qiang Liao
Chemical Engineering Journal.2024; 490: 151602. CrossRef
Numerical simulation of enzymatic hydrolysis in a bionic intestinal segmentation reactor
Qiang Liao, Tong Zhu, Chang Zhang, Yun Huang, Xianqing Zhu, Xun Zhu, Ao Xia
Physics of Fluids.2024;[Epub] CrossRef
Soil metaphenomics: a step forward in metagenomics
Muhammad Azeem, Parimala Gnana Soundari, Amjad Ali, Muhammad Ibrahim Tahir, Muhammad Imran, Saqib Bashir, Muhammad Irfan, Gang Li, Yong-Guan Zhu, Zenqqiang Zhang
Archives of Agronomy and Soil Science.2022; 68(12): 1645. CrossRef
Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective
Samaila Boyi Ajeje, Yun Hu, Guojie Song, Sunday Bulus Peter, Richmond Godwin Afful, Fubao Sun, Mohammad Ali Asadollahi, Hamid Amiri, Ali Abdulkhani, Haiyan Sun
Frontiers in Bioengineering and Biotechnology.2021;[Epub] CrossRef
Emerging priorities for microbial metagenome research
Rangasamy Kirubakaran, K.N. ArulJothi, Sundaravadivel Revathi, Nowsheen Shameem, Javid A. Parray
Bioresource Technology Reports.2020; 11: 100485. CrossRef
Lignocellulolytic systems of soil bacteria: A vast and diverse toolbox for biotechnological conversion processes
Rubén López-Mondéjar, Camelia Algora, Petr Baldrian
Biotechnology Advances.2019; 37(6): 107374. CrossRef
RETRACTED: Thermostable cellulases: Current status and perspectives
Anil K. Patel, Reeta Rani Singhania, Sang Jun Sim, Ashok Pandey
Bioresource Technology.2019; 279: 385. CrossRef
Recent Advances in Function-Based Metagenomic Screening
Tanyaradzwa Rodgers Ngara, Houjin Zhang
Genomics, Proteomics & Bioinformatics.2018; 16(6): 405. CrossRef
Bioprospecting of functional cellulases from metagenome for second generation biofuel production: a review
Rameshwar Tiwari, Lata Nain, Nikolaos E. Labrou, Pratyoosh Shukla
Critical Reviews in Microbiology.2018; 44(2): 244. CrossRef
Novel archaeal thermostable cellulases from an oil reservoir metagenome
Anna Lewin, Jinglie Zhou, Vu Thuy Trang Pham, Tone Haugen, Mohamed El Zeiny, Olav Aarstad, Wolfgang Liebl, Alexander Wentzel, Mark R. Liles
AMB Express.2017;[Epub] CrossRef
Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform
Kazuki Nakamura, Ryo Iizuka, Shinro Nishi, Takao Yoshida, Yuji Hatada, Yoshihiro Takaki, Ayaka Iguchi, Dong Hyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji, Takashi Funatsu
Scientific Reports.2016;[Epub] CrossRef
Revisiting cellulase production and redefining current strategies based on major challenges
Ramesh Chander Kuhad, Deepa Deswal, Sonia Sharma, Abhishek Bhattacharya, Kavish Kumar Jain, Amandeep Kaur, Brett I. Pletschke, Ajay Singh, Matti Karp
Renewable and Sustainable Energy Reviews.2016; 55: 249. CrossRef
Optimization of a heat‐tolerant β‐glucosidase production by Bacillus sp. ZJ1308 and its purification and characterization
Zhenni Xu, Lei Zhang, Ping Yu
Biotechnology and Applied Biochemistry.2016; 63(4): 553. CrossRef
Bg10: A Novel Metagenomics Alcohol-Tolerant and Glucose-Stimulated GH1 ß-Glucosidase Suitable for Lactose-Free Milk Preparation
Elisângela Soares Gomes-Pepe, Elwi Guillermo Machado Sierra, Mariana Rangel Pereira, Tereza Cristina Luque Castellane, Eliana Gertrudes de Macedo Lemos, Israel Silman
PLOS ONE.2016; 11(12): e0167932. CrossRef
Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening
María‐Eugenia Guazzaroni, Rafael Silva‐Rocha, Richard John Ward
Microbial Biotechnology.2015; 8(1): 52. CrossRef
New carbohydrate-active enzymes identified by screening two metagenomic libraries derived from the soil of a winter wheat field
A. Stroobants, D. Portetelle, M. Vandenbol
Journal of Applied Microbiology.2014; 117(4): 1045. CrossRef
Phylogenetic and Functional Analysis of Gut Microbiota of a Fungus-Growing Higher Termite: Bacteroidetes from Higher Termites Are a Rich Source of β-Glucosidase Genes
Meiling Zhang, Ning Liu, Changli Qian, Qianfu Wang, Qian Wang, Yanhua Long, Yongping Huang, Zhihua Zhou, Xing Yan
Microbial Ecology.2014; 68(2): 416. CrossRef
Evaluation of a Pooled Strategy for High-Throughput Sequencing of Cosmid Clones from Metagenomic Libraries
Kathy N. Lam, Michael W. Hall, Katja Engel, Gregory Vey, Jiujun Cheng, Josh D. Neufeld, Trevor C. Charles, Axel Cloeckaert
PLoS ONE.2014; 9(6): e98968. CrossRef
Discovery of two novel β-glucosidases from an Amazon soil metagenomic library
Jessica C. Bergmann, Ohana Yonara A. Costa, John M. Gladden, Steven Singer, Richard Heins, Patrik D'haeseleer, Blake A. Simmons, Betania F. Quirino
FEMS Microbiology Letters.2014; 351(2): 147. CrossRef
Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials
Annette Sørensen, Mette Lübeck, Peter Lübeck, Birgitte Ahring
Biomolecules.2013; 3(3): 612. CrossRef
Characterization of a Cold-Active β-Glucosidase from Paenibacillus xylanilyticus KJ-03 Capable of Hydrolyzing Isoflavones Daidzin and Genistin
Dong-Ju Park, Yong-Suk Lee, Yong-Lark Choi
The Protein Journal.2013; 32(7): 579. CrossRef
Application of metagenomic techniques in mining enzymes from microbial communities for biofuel synthesis
Mei-Ning Xing, Xue-Zhu Zhang, He Huang
Biotechnology Advances.2012; 30(4): 920. CrossRef
Cloning and Characterization of Two β-Glucosidase/Xylosidase Enzymes from Yak Rumen Metagenome
Lei Bao, Qiang Huang, Lei Chang, Qingwen Sun, Jungang Zhou, Hong Lu
Applied Biochemistry and Biotechnology.2012; 166(1): 72. CrossRef
Characterization of a novel thermostable β-glucosidase from a metagenomic library of termite gut
Qianfu Wang, Changli Qian, Xiao-Zhou Zhang, Nin Liu, Xing Yan, Zhihua Zhou
Enzyme and Microbial Technology.2012; 51(6-7): 319. CrossRef
Characterization of a Glucose-, Xylose-, Sucrose-, and d-Galactose-Stimulated β-Glucosidase from the Alkalophilic Bacterium Bacillus halodurans C-125
Hu Xu, Ai-Sheng Xiong, Wei Zhao, Yong-Sheng Tian, Ri-He Peng, Jian-Min Chen, Quan-Hong Yao
Current Microbiology.2011; 62(3): 833. CrossRef
Biochemical characterization of two novel β-glucosidase genes by metagenome expression cloning
Chengjian Jiang, Shuang-Xi Li, Feng-Feng Luo, Ke Jin, Qin Wang, Zhen-Yu Hao, Lan-Lan Wu, Gao-Chao Zhao, Ge-Fei Ma, Pei-Hong Shen, Xian-Lai Tang, Bo Wu
Bioresource Technology.2011; 102(3): 3272. CrossRef
Mining metagenomes for novel cellulase genes
Cheng-Jie Duan, Jia-Xun Feng
Biotechnology Letters.2010; 32(12): 1765. CrossRef
Identification of a metagenome-derived β-glucosidase from bioreactor contents
Chengjian Jiang, Zhen-Yu Hao, Ke Jin, Shuang-Xi Li, Zhi-Qun Che, Ge-Fei Ma, Bo Wu
Journal of Molecular Catalysis B: Enzymatic.2010; 63(1-2): 11. CrossRef

Journal Article

Analysis and Identification of ADP-Ribosylated Proteins of Streptomyces coelicolor M145: András Penyige , Judit Keser&# , Ferenc Fazakas , Iván Schmelczer , Krisztina Szirák , György Barabás , Sándor Biró; J. Microbiol. 2009;47(5):549-556. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0032-y

87 View
0 Download
11 Scopus

Abstract: Mono-ADP-ribosylation is the enzymatic transfer of ADP-ribose from NAD+ to acceptor proteins catalyzed by ADP-ribosyltransferases. Using m-aminophenylboronate affinity chromatography, 2D-gel electrophoresis, in-gel digestion and MALDI-TOF analysis we have identified eight in vitro ADP-ribosylated proteins in Streptomyces coelicolor, which can be classified into three categories: (i) secreted proteins; (ii) metabolic enzymes using NAD+/NADH or NADP+/NADPH as coenzymes; and (iii) other proteins. The secreted proteins could be classified into two functional categories: SCO2008 and SCO5477 encode members of the family of periplasmic extracellular solute-binding proteins, and SCO6108 and SCO1968 are secreted hydrolases. Dehydrogenases are encoded by SCO4824 and SCO4771. The other targets are GlnA (glutamine synthetaseI., SCO2198) and SpaA (starvation-sensing protein encoded by SCO7629). SCO2008 protein and GlnA had been identified as ADP-ribosylated proteins in previous studies. With these results we provided experimental support for a previous suggestion that ADP-ribosylation may regulate membrane transport and localization of periplasmic proteins. Since ADP-ribosylation results in inactivation of the target protein, ADP-ribosylation of dehydrogenases might modulate crucial primary metabolic pathways in Streptomyces. Several of the proteins identified here could provide a strong connection between protein ADP-ribosylation and the regulation of morphological differentiation in S. coelicolor.

Research Support, Non-U.S. Gov't

Overexpression of Outer Membrane Protein OprT and Increase of Membrane Permeability in phoU Mutant of Toluene-Tolerant Bacterium Pseudomonas putida GM730: Kyunghee Lee , Juna Jung , Kwang Kim , Dongwon Bae , Dongbin Lim; J. Microbiol. 2009;47(5):557-562. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0105-y

100 View
0 Download
4 Crossref

Abstract

Eight toluene-sensitive mutants were previously isolated from the toluene-tolerant bacterium Pseudomonas putida GM730. One of these mutants was TOS6, in which Tn5 had been inserted into phoU. Susceptibility to multiple antibiotics, as well as toluene sensitivity, was increased in the phoU mutant of P. putida GM730. We compared the outer membrane proteins from the phoU mutant and wild-type via two-dimensional gel electrophoresis. A 45 kDa protein was dramatically overexpressed as the result of phoU inactivation, and this protein was identified by peptide mass fingerprinting and microsequencing as a conserved hypothetical protein consisting of 414 amino acids. The protein, designated as OprT, harbors a signal sequence and extended β-sheets, both of which are features common to the bacterial porins. The rate of ethidium bromide accumulation in TOS6 was higher than in GM730, which indicates that the TOS6 membranes may be more permeable to ethidium bromide than are the membranes of GM730. We propose that the toluene sensitivity and increased antibiotic susceptibility observed in the phoU mutant may be attributable to increased membrane permeability.

Citations

Citations to this article as recorded by

Sublethal Paraquat Confers Multidrug Tolerance in Pseudomonas aeruginosa by Inducing Superoxide Dismutase Activity and Lowering Envelope Permeability
Dorival Martins, Geoffrey A. McKay, Ann M. English, Dao Nguyen
Frontiers in Microbiology.2020;[Epub] CrossRef
Superoxide dismutase activity confers (p)ppGpp-mediated antibiotic tolerance to stationary-phase Pseudomonas aeruginosa
Dorival Martins, Geoffrey McKay, Gowthami Sampathkumar, Malika Khakimova, Ann M. English, Dao Nguyen
Proceedings of the National Academy of Sciences.2018; 115(39): 9797. CrossRef
Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization
Xue LIU, Chang-xiong ZHU, Delvigne Frank, Ming-bo GONG
Journal of Integrative Agriculture.2017; 16(9): 2110. CrossRef
Crystal structure of PhoU from Pseudomonas aeruginosa, a negative regulator of the Pho regulon
Sang Jae Lee, Ye Seol Park, Soon-Jong Kim, Bong-Jin Lee, Se Won Suh
Journal of Structural Biology.2014; 188(1): 22. CrossRef

Journal Article

Response Surface Analysis for the Production of an Enantioselective Lipase from Aspergillus niger by Solid-State Fermentation: Fabiano Jares Contesini , Vania Castriani Fernades da Silva , Rafael Ferreira Maciel , Rosemary Joana de Lima , Francisco Fábio Cavalcante Barros , Patrícia de Oliveira Carvalho; J. Microbiol. 2009;47(5):563-571. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-008-0279-8

100 View
0 Download
30 Scopus

Abstract: The lipase produced by the Aspergillus niger strain AC-54 has been widely studied due to its enantioselectivity for racemic mixtures. This study aimed to optimize the production of this enzyme using statistical methodology. Initially a Plackett-Burman (PB) design was used to evaluate the effects of the culture medium components and the culture conditions. Twelve factors were screened: water content, glucose, yeast extract, peptone, olive oil, temperature, NaH2PO4, KH2PO4, MgSO4․7H2O, CaCl2, NaCl, and MnSO4. The screening showed that the significant factors were water content, glucose, yeast extract, peptone, NaH2PO4, and KH2PO4, which were optimized using response surface methodology (RSM) and a mathematical model obtained to explain the behavioral process. The best lipase activity was attained using the following conditions: water content (20%), glucose (4.8%), yeast extract (4.0%), and NaH2PO4 (4.0%). The predicted lipase activity was 33.03 U/ml and the experimental data confirmed the validity of the model. The enzymatic activity was expressed as µmoles of oleic acid released per minute of reaction (µmol/min).

Research Support, Non-U.S. Gov't

Ligand-Receptor Recognition for Activation of Quorum Sensing in Staphylococcus aureus: Li-Chun Chen , Li-Tse Tsou , Feng-Jui Chen; J. Microbiol. 2009;47(5):572-581. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0004-2

105 View
0 Download
12 Crossref

Abstract

The accessory gene regulator (agr) locus controls many of the virulence toxins involved in Staphylococcus aureus pathogenesis, and can be divided into four specificity groups. AgrC is the only group-specific receptor to mediate both intra-group activation and inter-group inhibition. We studied the ligand-receptor recognition of the agr system in depth by using a luciferase reporter system to identify the key residues responsible for AgrC activation in two closely related agr groups, AgrC-I, and AgrC-IV. Fusion PCR and site-directed mutagenesis were used to screen for functional residues of AgrC. Our data suggest that for AgrC-IV activation, residue 101 is critical for activating the receptor. In contrast, the key residues for the activation of AgrC-I are located at residues 49~59, 107, and 116. However, three residue changes, T101A, V107S, I116S, are sufficient to convert the AIP recognizing specificity from AgrC-IV to AgrC-I.

Citations

Citations to this article as recorded by

Virulence attributes of successful methicillin-resistant Staphylococcus aureus lineages
Jhih-Hang Jiang, David R. Cameron, Cara Nethercott, Marta Aires-de-Sousa, Anton Y. Peleg, Graeme N. Forrest
Clinical Microbiology Reviews.2023;[Epub] CrossRef
Proteoliposome‐based model for screening inhibitors targeting histidine kinase AgrC
Liying Zhang, Chunshan Quan, Xuning Zhang, Wen Xiong, Shengdi Fan
Chemical Biology & Drug Design.2019; 93(5): 712. CrossRef
Effect of a Point Mutation in mprF on Susceptibility to Daptomycin, Vancomycin, and Oxacillin in an MRSA Clinical Strain
Feng-Jui Chen, Tsai-Ling Lauderdale, Chen-Hsiang Lee, Yu-Chieh Hsu, I-Wen Huang, Pei-Chi Hsu, Chung-Shi Yang
Frontiers in Microbiology.2018;[Epub] CrossRef
Specificity and complexity in bacterial quorum-sensing systems
Lisa A. Hawver, Sarah A. Jung, Wai-Leung Ng, Aimee Shen
FEMS Microbiology Reviews.2016; 40(5): 738. CrossRef
An Electrostatic Interaction between BlpC and BlpH Dictates Pheromone Specificity in the Control of Bacteriocin Production and Immunity in Streptococcus pneumoniae
Marisa D. Pinchas, Nathan C. LaCross, Suzanne Dawid, G. A. O'Toole
Journal of Bacteriology.2015; 197(7): 1236. CrossRef
Role of the mecA Gene in Oxacillin Resistance in a Staphylococcus aureus Clinical Strain with a pvl -Positive ST59 Genetic Background
Feng-Jui Chen, Chen-Her Wang, Ching-Yi Chen, Yu-Chieh Hsu, Kaun-Ting Wang
Antimicrobial Agents and Chemotherapy.2014; 58(2): 1047. CrossRef
Origin and Evolution of European Community-Acquired Methicillin-Resistant Staphylococcus aureus
Marc Stegger, Thierry Wirth, Paal S. Andersen, Robert L. Skov, Anna De Grassi, Patricia Martins Simões, Anne Tristan, Andreas Petersen, Maliha Aziz, Kristoffer Kiil, Ivana Cirković, Edet E. Udo, Rosa del Campo, Jaana Vuopio-Varkila, Norazah Ahmad, Sima To
mBio.2014;[Epub] CrossRef
Green Fluorescent Protein (GFP)-Based Overexpression Screening and Characterization of AgrC, a Receptor Protein of Quorum Sensing in Staphylococcus aureus
Lina Wang, Chunshan Quan, Baoquan Liu, Yongbin Xu, Pengchao Zhao, Wen Xiong, Shengdi Fan
International Journal of Molecular Sciences.2013; 14(9): 18470. CrossRef
Functional Reconstitution of Staphylococcus aureus Truncated AgrC Histidine Kinase in a Model Membrane System
Lina Wang, Chunshan Quan, Baoquan Liu, Jianfeng Wang, Wen Xiong, Pengchao Zhao, Shengdi Fan, Gunnar F. Kaufmann
PLoS ONE.2013; 8(11): e80400. CrossRef
Peptide‐based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling
Nicholas Marchand, Cynthia H. Collins
Biotechnology and Bioengineering.2013; 110(11): 3003. CrossRef
Peptide Signaling in the Staphylococci
Matthew Thoendel, Jeffrey S. Kavanaugh, Caralyn E. Flack, Alexander R. Horswill
Chemical Reviews.2011; 111(1): 117. CrossRef
Structural Basis for Ligand Recognition and Discrimination of a Quorum-quenching Antibody
Robert N. Kirchdoerfer, Amanda L. Garner, Caralyn E. Flack, Jenny M. Mee, Alexander R. Horswill, Kim D. Janda, Gunnar F. Kaufmann, Ian A. Wilson
Journal of Biological Chemistry.2011; 286(19): 17351. CrossRef

Validation Study

Development and Validation of a Recombinant Nucleocapsid Protein-Based ELISA for Detection of the Antibody to Porcine Reproductive and Respiratory Syndrome Virus: Jia-Qi Chu , Xu-Min Hu , Myung-Cheol Kim , Chang-Sik Park , Moo-Hyung Jun; J. Microbiol. 2009;47(5):582-588. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0033-x

135 View
0 Download
13 Scopus

Abstract: Three indirect enzyme-linked immunosorbent assays (iELISA) based on the North American like (NA-like), European like (EU-like) and co-expressed NA- and EU-like recombinant nucleocapsid proteins (N-protein) of porcine reproductive and respiratory syndrome virus (PRRSV) were validated for the detection of the antibodies in porcine sera. A total of 422 serum samples from unvaccinated pigs were tested. The cut-off value was optimized by a two-graph receiver operating characteristics analysis at a 95% confidence level. This assay was validated with Western blot analysis and IDEXX HerdChek™ ELISA. Cross-reactivity results showed that iELISA was PRRSV-specific. Repeatability tests revealed that the coefficients of variation of positive sera within and between runs were less than 10%. The results indicate that iELISA is simpler to produce and perform, time-saving and suitable for large scale surveys of PRRSV infection at low cost, and is potentially useful to evaluate the efficiency of various vaccines against PRRSV.

Research Support, Non-U.S. Gov'ts

Identification of a Novel Linear B-Cell Epitope in the M Protein of Avian Infectious Bronchitis Coronaviruses: Junji Xing , Shengwang Liu , Zongxi Han , Yuhao Shao , Huixin Li , Xiangang Kong; J. Microbiol. 2009;47(5):589-599. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0104-z

102 View
0 Download
11 Crossref

Abstract

This report describes the identification of a novel linear B-cell epitope at the C-terminus of the membrane (M) protein of avian infectious bronchitis virus (IBV). A monoclonal antibody (MAb) (designated as 15E2) against the IBV M protein was prepared and a series of 14 partially-overlapping fragments of the IBV M gene were expressed with a GST tag. These peptides were subjected to enzyme-linked immunosorbent assay (ELISA) and western blotting analysis using MAb 15E2 to identify the epitope. A linear motif, 199FATFVYAK206, which was located at the C-terminus of the M protein, was identified by MAb 15E2. ELISA and western blotting also showed that this epitope could be recognized by IBV-positive serum from chicken. Given that 15E2 showed reactivity with the 199FATFVYAK206 motif, expressed as a GST fusion protein, in both western blotting and in an ELISA, we proposed that this motif represented a linear B-cell epitope of the M protein. The 199FATFVYAK206 motif was the minimal requirement for reactivity as demonstrated by analysis of the reactivity of 15E2 with several truncated peptides that were derived from the motif. Alignment and comparison of the 15E2-defined epitope sequence with the sequences of other coronaviruses indicated that the epitope is well conserved among chicken and turkey coronaviruses. The identified epitope should be useful in clinical applications and as a tool for the further study of the structure and function of the M protein of IBV.

Citations

Citations to this article as recorded by

A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs
Francisco Jesus Castañeda-Montes, José Luis Cerriteño-Sánchez, María Azucena Castañeda-Montes, Julieta Sandra Cuevas-Romero, Susana Mendoza-Elvira
Viruses.2023; 15(5): 1049. CrossRef
Predicted 3D model of the M protein of Porcine Epidemic Diarrhea Virus and analysis of its immunogenic potential
Alan Rodríguez-Enríquez, Irma Herrera-Camacho, Lourdes Millán-Pérez-Peña, Julio Reyes-Leyva, Gerardo Santos-López, José Francisco Rivera-Benítez, Nora Hilda Rosas-Murrieta, Claude Krummenacher
PLOS ONE.2022; 17(2): e0263582. CrossRef
Genetic and antigenic heterogeneity of infectious bronchitis virus in South America: implications for control programmes
Ana Marandino, Ariel Vagnozzi, María Isabel Craig, Gonzalo Tomás, Claudia Techera, Yanina Panzera, Federico Vera, Ruben Pérez
Avian Pathology.2019; 48(3): 270. CrossRef
Prediction and identification of novel IBV S1 protein derived CTL epitopes in chicken
Lei Tan, Ying Liao, Jin Fan, Yuqiang Zhang, Xiang Mao, Yingjie Sun, Cuiping Song, Xusheng Qiu, Chunchun Meng, Chan Ding
Vaccine.2016; 34(3): 380. CrossRef
Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection
Lei Tan, Yuqiang Zhang, Fang Liu, Yanmei Yuan, Yuan Zhan, Yingjie Sun, Xusheng Qiu, Chunchun Meng, Cuiping Song, Chan Ding
Vaccine.2016; 34(44): 5209. CrossRef
Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein
Hui Dong, Xin Zhang, Hongyan Shi, Jianfei Chen, Da Shi, Yunnuan Zhu, Li Feng
Viruses.2016; 8(12): 327. CrossRef
Development of an ELISA based on a multi-fragment antigen of infectious bronchitis virus for antibodies detection
Meng-die Ding, Xin Yang, Hong-ning Wang, An-yun Zhang, Zhi-kun Zhang, Wen-qiao Fan, Hai-peng Cao
Biotechnology Letters.2015; 37(12): 2453. CrossRef
Two novel neutralizing antigenic epitopes of the s1 subunit protein of a QX-like avian infectious bronchitis virus strain Sczy3 as revealed using a phage display peptide library
Nianli Zou, Jing Xia, Fuyan Wang, Zhenzhen Duan, Dan Miao, Qigui Yan, Sanjie Cao, Xintian Wen, Ping Liu, Yong Huang
Veterinary Immunology and Immunopathology.2015; 168(1-2): 49. CrossRef
Adaptation and Attenuation of Duck Tembusu Virus Strain Du/CH/LSD/110128 following Serial Passage in Chicken Embryos
Ling Sun, Yunxia Li, Yue Zhang, Zongxi Han, Yang Xu, Xiangang Kong, Shengwang Liu, R. L. Hodinka
Clinical and Vaccine Immunology.2014; 21(8): 1046. CrossRef
Identification of a conserved linear B-cell epitope in the M protein of porcine epidemic diarrhea virus
Zhibang Zhang, Jianfei Chen, Hongyan Shi, Xiaojin Chen, Da Shi, Li Feng, Bin Yang
Virology Journal.2012;[Epub] CrossRef
Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus
Xiaoli Liu, Zongxi Han, Yuhao Shao, Dan Yu, Huixin Li, Yu Wang, Xiangang Kong, Shengwang Liu
Virology Journal.2010;[Epub] CrossRef

Production of and Applications for a Polyclonal IgY Diagnostic Reagent Specific for Mycobacterium avium subsp. paratuberculosis: Sung Jae Shin , Seung-Sub Lee , Elizabeth J. B. Manning , Michael T. Collins; J. Microbiol. 2009;47(5):600-609. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0052-7

101 View
0 Download
6 Crossref

Abstract

Antibodies specific to the cell surface antigens of Mycobacterium avium subsp. paratuberculosis (MAP) have multiple useful applications, e.g. organism detection, immunoconcentration, and cell visualization. The aim of this study was to produce and compare polyclonal antibodies for such research and diagnostic purposes. Three polyclonal antibodies to MAP were produced using sera from immunized rabbits and chickens plus naturally infected cows. Cross-reactive antibodies in each MAP antibody preparation were removed by absorption with heterologous mycobacterial and non-mycobacterial cells. The specificity of each resulting polyclonal antibody preparation was evaluated by ELISA to multiple bacterial cell wall extract antigens. After absorption, chicken anti-MAP IgY had the highest specificity of the three antibody preparations. FITC-labeled anti-MAP IgY was used to effectively locate MAP in macrophages 12 h post-infection. Also, immuno- magnetic beads coated with anti-MAP IgY enhanced recovery of MAP from bacterial suspensions in comparison with non-antibody coated beads. Anti-MAP IgY provides a novel new reagent with broad diagnostic and research applications requiring specific concentration, detection, and quantification of MAP.

Citations

Citations to this article as recorded by

Detection of Mycobacterium avium Subspecies paratuberculosis (MAP) Microorganisms Using Antigenic MAP Cell Envelope Proteins
Shanmugasundaram Karuppusamy, Lucy Mutharia, David Kelton, Brandon Plattner, Sanjay Mallikarjunappa, Niel Karrow, Gordon Kirby
Frontiers in Veterinary Science.2021;[Epub] CrossRef
Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
Gentil Arthur Bentes, Natália Maria Lanzarini, Lyana Rodrigues Pinto Lima, Pedro Paulo de Abreu Manso, Alexandre dos Santos da Silva, Sergio da Silva e Mouta Junior, Juliana Rodrigues Guimarães, Marcia Terezinha Baroni de Moraes, Marcelo Pelajo-Machado, M
Memórias do Instituto Oswaldo Cruz.2015; 110(4): 577. CrossRef
An immunological method for granulovirus detection in larvae ofTuta absoluta: searching for isolates with prospects for biological control of this pest in Colombia
Juliana Gómez-Valderrama, Lorena Herrera, Daniel Uribe-Vélez, Miguel López-Ferber, Laura Villamizar
International Journal of Pest Management.2014; 60(2): 136. CrossRef
Recent research on bovine paratuberculosis in South Korea
Han Sang Yoo, Sung Jae Shin
Veterinary Immunology and Immunopathology.2012; 148(1-2): 23. CrossRef
Identification and profiling of circulating antigens by screening with the sera from schistosomiasis japonica patients
Yan Lu, Bin Xu, Chuan Ju, Xiaojin Mo, Shenbo Chen, Zheng Feng, Xiaoning Wang, Wei Hu
Parasites & Vectors.2012;[Epub] CrossRef
Chicken Monoclonal IgY Antibody: A Novel Antibody Development Strategy
Xiaoying Zhang, Hongxiu Chen, Zehua Tian, Shulin Chen, Rüdiger Schade
Avian Biology Research.2010; 3(3): 97. CrossRef

Retracted Publication

Identification and Characterization of a Novel Antibacterial Peptide, Avian β-Defensin 2 from Ducks: Deying Ma , Ruiqin Wang , Wenyan Liao , Zongxi Han , Shengwang Liu; J. Microbiol. 2009;47(5):610-618. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0068-z

100 View
0 Download
23 Crossref

Abstract

In this study, a novel avian β-defensin (AvBD) was isolated from duck pancreas. The complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. Homology, characterization and comparison of the gene with AvBD from other avian species confirmed that it was duck AvBD2. The mRNA expression of the gene was analyzed in 17 tissues from 21-day-old ducks. AvBD2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed in the muscular stomach, small intestine, kidney, spleen, thymus, and bursa of Fabricius; and weakly expressed in skin. We produced and purified recombinant AvBD2 by expressing the gene in Escherichia coli. As expected, the recombinant peptide exhibited strong bactericidal properties against Bacillus cereus, Staphylococcus aureus, and Pasteurella multocida, and weak bactericidal properties against E. coli and Salmonella choleraesuis. In addition, the recombinant protein retained antimicrobial activity against S. aureus under different temperatures (range, -20°C to 100°C) and pH values (range, 3 to 12).

Citations

Citations to this article as recorded by

Utilization of Antiviral Peptides in Treating Coronavirus Infection: An Overview
Qian Xu, Fangyu Wang, Hua Feng, Qiang Wei, Xuefeng Sun, Guangxu Xing, Gaiping Zhang
International Journal of Peptide Research and Therapeutics.2024;[Epub] CrossRef
Application of antimicrobial peptide Mytichitin‐A in pork preservation during refrigerated storage
De‐Mei Meng, Xiao‐Min Yang, Xue‐Qing Sun, Lei Cheng, Zhen‐Chuan Fan
Journal of Food Processing and Preservation.2021;[Epub] CrossRef
Effect of Bifunctional β Defensin 2-Modified Scaffold on Bone Defect Reconstruction
Yiyu Peng, Lunhao Li, Qingyue Yuan, Ping Gu, Zhengwei You, Ai Zhuang, Xiaoping Bi
ACS Omega.2020; 5(8): 4302. CrossRef
Antioxidant activities and protective effects of duck embryo peptides against H2 O2-induced oxidative damage in HepG2 cells
Ying He, Lijun Bu, Huadong Xie, Guizhao Liang
Poultry Science.2019; 98(12): 7118. CrossRef
Efficient production of a recombinant Venerupis philippinarum defensin (VpDef) in Pichia pastoris and characterization of its antibacterial activity and stability
De-Mei Meng, Yu-Jie Lv, Jing-Fang Zhao, Qing-Yan Liu, Lin-Yue Shi, Jun-Ping Wang, Yong-Hai Yang, Zhen-Chuan Fan
Protein Expression and Purification.2018; 147: 78. CrossRef
Expression of Immune-Related Genes of Ducks Infected with Avian Pathogenic Escherichia coli (APEC)
Rong Li, Ning Li, Jinzhou Zhang, Yao Wang, Jiyuan Liu, Yumei Cai, Tongjie Chai, Liangmeng Wei
Frontiers in Microbiology.2016;[Epub] CrossRef
In vitro and in vivo characterization of a new recombinant antimicrobial peptide, MP1102, against methicillin-resistant Staphylococcus aureus
Yong Zhang, Da Teng, Xiumin Wang, Ruoyu Mao, Xintao Cao, Xiaoyuan Hu, Lifen Zong, Jianhua Wang
Applied Microbiology and Biotechnology.2015; 99(15): 6255. CrossRef
A dual mechanism involved in membrane and nucleic acid disruption of AvBD103b, a new avian defensin from the king penguin, against Salmonella enteritidis CVCC3377
Da Teng, Xiumin Wang, Di Xi, Ruoyu Mao, Yong Zhang, Qingfeng Guan, Jun Zhang, Jianhua Wang
Applied Microbiology and Biotechnology.2014; 98(19): 8313. CrossRef
Molecular cloning, characterization and tissue distribution of two ostrich β-defensins: AvBD2 and AvBD7
Shun Lu, Kemei Peng, Qishuang Gao, Min Xiang, Huazhen Liu, Hui Song, Keli Yang, Haibo Huang, Ke Xiao
Gene.2014; 552(1): 1. CrossRef
High expression of a plectasin-derived peptide NZ2114 in Pichia pastoris and its pharmacodynamics, postantibiotic and synergy against Staphylococcus aureus
Yong Zhang, Da Teng, Ruoyu Mao, Xiumin Wang, Di Xi, Xiaoyuan Hu, Jianhua Wang
Applied Microbiology and Biotechnology.2014; 98(2): 681. CrossRef
Tissue distribution, expression, and antimicrobial activity of Anas platyrhynchos avian β-defensin 6
K.S. Peng, L.S. Ruan, J. Tu, K.Z. Qi, L.H. Jiang
Poultry Science.2013; 92(1): 97. CrossRef
Identification of three novel avian beta-defensins from goose and their significance in the pathogenesis of Salmonella
Deying Ma, Mingyue Zhang, Kexin Zhang, Xiaoli Liu, Zongxi Han, Yuhao Shao, Shengwang Liu
Molecular Immunology.2013; 56(4): 521. CrossRef
Cardiac beta-defensins upregulate with a high fat diet and influence monocyte migration
Annika Linde, Christopher Ross, Frank Blecha, Gerald H. Lushington, Tonatiuh Melgarejo
Open Journal of Internal Medicine.2013; 03(02): 81. CrossRef
Avian host defense peptides
Tryntsje Cuperus, Maarten Coorens, Albert van Dijk, Henk P. Haagsman
Developmental & Comparative Immunology.2013; 41(3): 352. CrossRef
Cloning, expression and characterization of antimicrobial porcine β defensin 1 in Escherichia coli
Chun-li Li, Ting-ting Xu, Rui-bo Chen, Xian-xian Huang, Yan-cong Zhao, Yuan-yuan Bao, Wei-dong Zhao, Zhen-yu Zheng
Protein Expression and Purification.2013; 88(1): 47. CrossRef
Identification, Expression and Activity Analyses of Five Novel Duck Beta-Defensins
Deying Ma, Kexin Zhang, Mingyue Zhang, Shengnan Xin, Xiaoli Liu, Zongxi Han, Yuhao Shao, Shengwang Liu, Tavis John E
PLoS ONE.2012; 7(10): e47743. CrossRef
Discovery and characterization of Coturnix chinensis avian β‐defensin 10, with broad antibacterial activity
Deying Ma, Lijuan Lin, Kexin Zhang, Zongxi Han, Yuhao Shao, Ruiqin Wang, Shengwang Liu
Journal of Peptide Science.2012; 18(4): 224. CrossRef
Functional analysis and induction of four novel goose (Anser cygnoides) avian β-defensins in response to salmonella enteritidis infection
Deying Ma, Caiyuan Zhou, Mingyue Zhang, Zongxi Han, Yuhao Shao, Shengwang Liu
Comparative Immunology, Microbiology and Infectious Diseases.2012; 35(2): 197. CrossRef
Three novel Anas platyrhynchos avian β-defensins, upregulated by duck hepatitis virus, with antibacterial and antiviral activities
Deying Ma, Lijuan Lin, Kexin Zhang, Zongxi Han, Yuhao Shao, Xiaoli Liu, Shengwang Liu
Molecular Immunology.2011; 49(1-2): 84. CrossRef
Animal contact as a source of human non-typhoidal salmonellosis
Karin Hoelzer, Andrea Moreno Switt, Martin Wiedmann
Veterinary Research.2011; 42(1): 34. CrossRef
Expression of plectasin in Pichia pastoris and its characterization as a new antimicrobial peptide against Staphyloccocus and Streptococcus
Jun Zhang, Yalin Yang, Da Teng, Zigang Tian, Shaoran Wang, Jianhua Wang
Protein Expression and Purification.2011; 78(2): 189. CrossRef
Identification and characterization of an avian β-defensin orthologue, avian β-defensin 9, from quails
Ruiqin Wang, Deying Ma, Lijuan Lin, Caiyuan Zhou, Zongxi Han, Yuhao Shao, Wenyan Liao, Shengwang Liu
Applied Microbiology and Biotechnology.2010; 87(4): 1395. CrossRef
Biologically active substances of bird skin: a review
J. Rajchard
Veterinární medicína.2010; 55(9): 413. CrossRef

Research Support, Non-U.S. Gov'ts

The Photodynamic Effect of Methylene Blue and Toluidine Blue on Candida albicans Is Dependent on Medium Conditions: Gabriela Guimarães Carvalho , Monalisa Poliana Felipe , Maricilia Silva Costa; J. Microbiol. 2009;47(5):619-623. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0059-0

100 View
0 Download
41 Crossref

Abstract

Due to the increased number of immunocompromised patients, the infections associated with the pathogen of the genus Candida and other fungi have increased dramatically. Photodynamic antimicrobial chemotherapy (PACT) has been presented as a potential antimicrobial therapy, in a process that combines light and a photosensitizing drug, which promotes a phototoxic response by the treated cells. In this work, we studied the effects of the different medium conditions during PACT, using either methylene blue (MB) or toluidine blue (TB) on Candida albicans. The inhibition of the growth produced by PACT was decreased for different pH values (6.0, 7.0, and 8.0) in a buffered medium. The phototoxic effects were observed only in the presence of saline (not buffered medium). PACT was modulated by calcium in a different manner using either MB or TB. Also when using MB both verapamil or sodium azide were able to decrease the phototoxic effects on the C. albicans. These results show that PACT is presented as a new and promising antifungal therapy, however, new studies are necessary to understand the mechanism by which this event occurs.

Citations

Citations to this article as recorded by

Antimicrobial Photodynamic Therapy for Superficial, Skin, and Mucosal Fungal Infections: An Update
Laura Beatriz Borim da Silva, Ivana Giovannetti Castilho, Fabiana Alves de Souza Silva, Mahmoud Ghannoum, Maíra Terra Garcia, Paulo Henrique Fonseca do Carmo
Microorganisms.2025; 13(6): 1406. CrossRef
What Is the Impact of Antimicrobial Photodynamic Therapy on Oral Candidiasis? An In Vitro Study
Emira D’Amico, Silvia Di Lodovico, Tania Vanessa Pierfelice, Domenico Tripodi, Adriano Piattelli, Giovanna Iezzi, Morena Petrini, Simonetta D’Ercole
Gels.2024; 10(2): 110. CrossRef
Antimicrobial efficacy of solar disinfection in cellulose fiber supported photoactive materials
Daniel Langerreiter, Katariina Solin, Mireia Jordà-Redondo, Roger Bresolí-Obach, Lukas Fliri, Santi Nonell, Mauri A. Kostiainen, Eduardo Anaya-Plaza
Materials Today Communications.2024; 38: 107858. CrossRef
The role of the light source in antimicrobial photodynamic therapy
Marta Piksa, Cheng Lian, Imogen C. Samuel, Krzysztof J. Pawlik, Ifor D. W. Samuel, Katarzyna Matczyszyn
Chemical Society Reviews.2023; 52(5): 1697. CrossRef
Reactive oxygen and nitrogen species are crucial for the antifungal activity of amorolfine and ciclopirox olamine against the dermatophyte Trichophyton interdigitale
Paulo H F Carmo, Gustavo J C Freitas, João C M Dornelas, Bruna C T Almeida, Ludmila M Baltazar, Gabriella F Ferreira, Nalu T A Peres, Daniel A Santos
Medical Mycology.2022;[Epub] CrossRef
A Novel Technique for Disinfection Treatment of Contaminated Dental Implant Surface Using 0.1% Riboflavin and 445 nm Diode Laser—An In Vitro Study
Luka Morelato, Ana Budimir, Igor Smojver, Ivan Katalinić, Marko Vuletić, Muhamed Ajanović, Dragana Gabrić
Bioengineering.2022; 9(7): 308. CrossRef
Visible Lights Combined with Photosensitizing Compounds Are Effective against Candida albicans Biofilms
Priyanka Bapat, Gurbinder Singh, Clarissa J. Nobile
Microorganisms.2021; 9(3): 500. CrossRef
Influence of Incubation Time on Ortho-Toluidine Blue Mediated Antimicrobial Photodynamic Therapy Directed against Selected Candida Strains—An In Vitro Study
Rafał Wiench, Joanna Nowicka, Magdalena Pajączkowska, Piotr Kuropka, Dariusz Skaba, Anna Kruczek-Kazibudzka, Anna Kuśka-Kiełbratowska, Kinga Grzech-Leśniak
International Journal of Molecular Sciences.2021; 22(20): 10971. CrossRef
Photodynamic inactivation of Lasiodiplodia theobromae : lighting the way towards an environmentally friendly phytosanitary treatment
M. Garcia, B. David, I. N. Sierra-Garcia, M. A. F. Faustino, A. Alves, A. C. Esteves, A. Cunha
Biology Letters.2021;[Epub] CrossRef
Photosensitizers Mediated Photodynamic Inactivation against Fungi
Daniel Ziental, Dariusz T. Mlynarczyk, Beata Czarczynska-Goslinska, Konrad Lewandowski, Lukasz Sobotta
Nanomaterials.2021; 11(11): 2883. CrossRef
Efficacy of Toluidine Blue—Mediated Antimicrobial Photodynamic Therapy on Candida spp. A Systematic Review
Rafał Wiench, Dariusz Skaba, Jacek Matys, Kinga Grzech-Leśniak
Antibiotics.2021; 10(4): 349. CrossRef
Photodynamic Therapy Is Effective Against Candida auris Biofilms
Priyanka S. Bapat, Clarissa J. Nobile
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Development and in vitro evaluation of buccal mucoadhesive films for photodynamic inactivation of Candida albicans
Camila Felix Vecchi, Rafaela Said dos Santos, Jéssica Bassi da Silva, Hélen Cassia Rosseto, Karina Mayumi Sakita, Terezinha Inez Estivalet Svidzinski, Patrícia de Souza Bonfim-Mendonça, Marcos Luciano Bruschi
Photodiagnosis and Photodynamic Therapy.2020; 32: 101957. CrossRef
Newly formulated 5% 5-aminolevulinic acid photodynamic therapy on Candida albicans
Giuseppe Greco, Simone Di Piazza, Jiemei Chan, Mirca Zotti, Reem Hanna, Ezio Gheno, Angelina O. Zekiy, Claudio Pasquale, Nicola De Angelis, Andrea Amaroli
Photodiagnosis and Photodynamic Therapy.2020; 29: 101575. CrossRef
Efficient in vitro photodynamic inactivation using repetitive light energy density on Candida albicans and Trichophyton mentagrophytes
S.A. Torres-Hurtado, J. Ramírez-Ramírez, A.C. Larios-Morales, J.C. Ramírez-San-Juan, R. Ramos-García, A.P. Espinosa-Texis, T. Spezzia-Mazzocco
Photodiagnosis and Photodynamic Therapy.2019; 26: 203. CrossRef
Photodynamic Antimicrobial Chemotherapy (PACT) using methylene blue inhibits the viability of the biofilm produced by Candida albicans
Leonardo de Carvalho Leonel, Moisés Lopes Carvalho, Bruna Marques da Silva, Stella Zamuner, Carlos Alberto-Silva, Maricilia Silva Costa
Photodiagnosis and Photodynamic Therapy.2019; 26: 316. CrossRef
Photodynamic Inactivation of Candida albicans in Blood Plasma and Whole Blood
Vera Sousa, Ana T. P. C. Gomes, Américo Freitas, Maria A. F. Faustino, Maria G. P. M. S. Neves, Adelaide Almeida
Antibiotics.2019; 8(4): 221. CrossRef
Photodynamic Antimicrobial Chemotherapy (PACT), using Toluidine blue O inhibits the viability of biofilm produced by Candida albicans at different stages of development
Ana Paula Pinto, Isabela Bueno Rosseti, Moisés Lopes Carvalho, Bruna Graziele Marques da Silva, Carlos Alberto-Silva, Maricilia Silva Costa
Photodiagnosis and Photodynamic Therapy.2018; 21: 182. CrossRef
Photodynamic antimicrobial chemotherapy (PACT) using toluidine blue inhibits both growth and biofilm formation by Candida krusei
Bruna Graziele Marques da Silva, Moisés Lopes Carvalho, Isabela Bueno Rosseti, Stella Zamuner, Maricilia Silva Costa
Lasers in Medical Science.2018; 33(5): 983. CrossRef
Light treatments of nail fungal infections
Jessica Houang, Gabriel Perrone, Damia Mawad, Philip C. Boughton, Andrew J. Ruys, Antonio Lauto
Journal of Biophotonics.2018;[Epub] CrossRef
Controlling methylene blue aggregation: a more efficient alternative to treat Candida albicans infections using photodynamic therapy
Gabriela Alves da Collina, Fernanda Freire, Thabata Paulino da Costa Santos, Natalia Gesse Sobrinho, Simone Aquino, Renato Araújo Prates, Daniela de Fátima Teixeira da Silva, Anna Carolina Ratto Tempestini Horliana, Christiane Pavani
Photochemical & Photobiological Sciences.2018; 17(10): 1355. CrossRef
Oral hygiene in intensive care unit patients with photodynamic therapy: study protocol for randomised controlled trial
Gabriela Alves Da Collina, Anna Carolina Ratto Tempestini-Horliana, Daniela de Fátima Teixeira da Silva, Priscila Larcher Longo, Maria Luisa Faria Makabe, Christiane Pavani
Trials.2017;[Epub] CrossRef
Spray layer-by-layer films for photodynamic inactivation
Kevin F. dos Santos, Romário J. da Silva, Karla B. Romio, Paula C.S. Souto, Josmary R. Silva, Nara C. de Souza
Photodiagnosis and Photodynamic Therapy.2016; 15: 197. CrossRef
Current trends in outwitting resistance development inCandidainfections through photodynamic and short peptide therapies: a strategic-shift from conventional antifungal agents
Bengyella Louis, Sayanika Devi Waikhom, Peter W. Atadja
Expert Review of Anti-infective Therapy.2016; 14(3): 345. CrossRef
Effect of 5-aminolevulinic acid photodynamic therapy on Candida albicans biofilms: An in vitro study
Hang Shi, Jiyang Li, Hui Zhang, Jie Zhang, Hongying Sun
Photodiagnosis and Photodynamic Therapy.2016; 15: 40. CrossRef
A novel set of symmetric methylene blue derivatives exhibits effective bacteria photokilling — a structure — response study
Anita Gollmer, Ariane Felgenträger, Wolfgang Bäumler, Tim Maisch, Andreas Späth
Photochemical & Photobiological Sciences.2015; 14(2): 335. CrossRef
Antimicrobial photodynamic therapy: an effective alternative approach to control fungal infections
Ludmila M. Baltazar, Anjana Ray, Daniel A. Santos, PatrÃcia S. Cisalpino, Adam J. Friedman, Joshua D. Nosanchuk
Frontiers in Microbiology.2015;[Epub] CrossRef
Gold Nanoparticle-Photosensitizer Conjugate Based Photodynamic Inactivation of Biofilm Producing Cells: Potential for Treatment of C. albicans Infection in BALB/c Mice
Mohd. Asif Sherwani, Saba Tufail, Aijaz Ahmed Khan, Mohammad Owais, Joy Sturtevant
PLOS ONE.2015; 10(7): e0131684. CrossRef
Photodynamic antimicrobial chemotherapy (PACT) inhibits biofilm formation by Candida albicans, increasing both ROS production and membrane permeability
Isabela Bueno Rosseti, Luciene Reginato Chagas, Maricilia Silva Costa
Lasers in Medical Science.2014; 29(3): 1059. CrossRef
Hydrogen Bond Acceptors and Additional Cationic Charges in Methylene Blue Derivatives: Photophysics and Antimicrobial Efficiency
Ariane Felgenträger, Tim Maisch, Daniel Dobler, Andreas Späth
BioMed Research International.2013; 2013: 1. CrossRef
Photodynamic inactivation of Candida albicans mediated by a low density of light energy
Gabriel Salles Barbério, Soraia Veloso da Costa, Mariana dos Santos Silva, Thaís Marchini de Oliveira, Thiago Cruvinel Silva, Maria Aparecida de Andrade Moreira Machado
Lasers in Medical Science.2013;[Epub] CrossRef
Photodynamic inhibition of Trichophyton rubrum: in vitro activity and the role of oxidative and nitrosative bursts in fungal death
L. d. M. Baltazar, B. M. Soares, H. C. S. Carneiro, T. V. Avila, L. F. Gouveia, D. G. Souza, M. V. L. Ferreira, M. Pinotti, D. d. A. Santos, P. S. Cisalpino
Journal of Antimicrobial Chemotherapy.2013; 68(2): 354. CrossRef
The impact of antimicrobial photodynamic therapy in an artificial biofilm model
Martin Schneider, Gregor Kirfel, Michael Berthold, Matthias Frentzen, Felix Krause, Andreas Braun
Lasers in Medical Science.2012; 27(3): 615. CrossRef
Paradoxical potentiation of methylene blue-mediated antimicrobial photodynamic inactivation by sodium azide: Role of ambient oxygen and azide radicals
Liyi Huang, Tyler G St. Denis, Yi Xuan, Ying-Ying Huang, Masamitsu Tanaka, Andrzej Zadlo, Tadeusz Sarna, Michael R. Hamblin
Free Radical Biology and Medicine.2012; 53(11): 2062. CrossRef
Miconazole Induces Fungistasis and Increases Killing of Candida albicans Subjected to Photodynamic Therapy†
Sara B. Snell, Thomas H. Foster, Constantine G. Haidaris
Photochemistry and Photobiology.2012; 88(3): 596. CrossRef
In Vitro Fungicidal Photodynamic Effect of Hypericin on Candida Species†
Antonio Rezusta, Patricia López‐Chicón, Manuel P. Paz‐Cristobal, Mireia Alemany‐Ribes, David Royo‐Díez, Montserrat Agut, Carlos Semino, Santi Nonell, Mª Jose Revillo, Carmen Aspiroz, Yolanda Gilaberte
Photochemistry and Photobiology.2012; 88(3): 613. CrossRef
Mechanistic Aspects of Photoinactivation of Candida albicans by Exogenous Porphyrins†
Sarit Oriel, Yeshayahu Nitzan
Photochemistry and Photobiology.2012; 88(3): 604. CrossRef
Photodynamic Antimicrobial Chemotherapy in Aquaculture: Photoinactivation Studies of Vibrio fischeri
Eliana Alves, Maria A. F. Faustino, João P. C. Tomé, Maria G. P. M. S. Neves, Augusto C. Tomé, José A. S. Cavaleiro, Ângela Cunha, Newton C. M. Gomes, Adelaide Almeida, Mark Alexander Webber
PLoS ONE.2011; 6(6): e20970. CrossRef
Effective photosensitization and selectivity In Vivo of Candida Albicans by meso‐tetra (N‐methyl‐4‐pyridyl) porphine tetra tosylate
Soumya Mitra, Constantine G. Haidaris, Sara B. Snell, Benjamin R. Giesselman, Steven M. Hupcher, Thomas H. Foster
Lasers in Surgery and Medicine.2011; 43(4): 324. CrossRef
Effect of pH on Methylene Blue Transient States and Kinetics and Bacteria Photoinactivation
Jie Chen, Thomas C. Cesario, Peter M. Rentzepis
The Journal of Physical Chemistry A.2011; 115(13): 2702. CrossRef
Susceptibility of Candida albicans to photodynamic action of 5,10,15,20-tetra(4-N-methylpyridyl)porphyrin in different media
Ezequiel D. Quiroga, María Gabriela Alvarez, Edgardo N. Durantini
FEMS Immunology & Medical Microbiology.2010; 60(2): 123. CrossRef

Identification of the Vibrio vulnificus ahpC1 Gene and Its Influence on Survival under Oxidative Stress and Virulence: Woon Ki Baek , Hyun Sung Lee , Man Hwan Oh , Myung Jin Koh , Kun-Soo Kim , Sang Ho Choi; J. Microbiol. 2009;47(5):624-632. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0130-x

114 View
0 Download
13 Crossref

Abstract

Pathogens have evolved sophisticated mechanisms to survive oxidative stresses imposed by host defense systems, and the mechanisms are closely linked to their virulence. In the present study, ahpC1, a homologue of Escherichia coli ahpC encoding a peroxiredoxin, was identified among the Vibrio vulnificus genes specifically induced by exposure to H2O2. In order to analyze the role of AhpC1 in the pathogenesis of V. vulnificus, a mutant, in which the ahpC1 gene was disrupted, was constructed by allelic exchanges. The ahpC1 mutant was hypersusceptable to killing by reactive oxygen species (ROS) such as H2O2 and t-BOOH, which is one of the most commonly used hydroperoxides in vitro. The purified AhpC1 reduced H2O2 in the presence of AhpF and NADH as a hydrogen donor, indicating that V. vulnificus AhpC1 is a NADH-dependent peroxiredoxin and constitutes a peroxide reductase system with AhpF. Compared to wild type, the ahpC1 mutant exhibited less cytotoxicity toward INT-407 epithelial cells in vitro and reduced virulence in a mouse model. In addition, the ahpC1 mutant was significantly diminished in growth with INT-407 epithelial cells, reflecting that the ability of the mutant to grow, survive, and persist during infection is also impaired. Consequently, the combined results suggest that AhpC1 and the capability of resistance to oxidative stresses contribute to the virulence of V. vulnificus by assuring growth and survival during infection.

Citations

Citations to this article as recorded by

Exploring functional and structural features of chemically related natural prenylated hydroquinone and benzoic acid from Piper crassinervium (Piperaceae) on bacterial peroxiredoxin inhibition
Vitoria Isabela Montanhero Cabrera, Gabrielle do Nascimento Sividanes, Natalia Fernanda Quintiliano, Marcos Hikari Toyama, João Henrique Ghilardi Lago, Marcos Antonio de Oliveira, A. Ganesan
PLOS ONE.2023; 18(2): e0281322. CrossRef
Relevance of peroxiredoxins in pathogenic microorganisms
Marcos Antonio de Oliveira, Carlos A. Tairum, Luis Eduardo Soares Netto, Ana Laura Pires de Oliveira, Rogerio Luis Aleixo-Silva, Vitoria Isabela Montanhero Cabrera, Carlos A. Breyer, Melina Cardoso dos Santos
Applied Microbiology and Biotechnology.2021; 105(14-15): 5701. CrossRef
Vibrio vulnificus RtxA Is a Major Factor Driving Inflammatory T Helper Type 17 Cell Responses in vitro and in vivo
Arim Lee, Myun Soo Kim, Daeho Cho, Kyung Ku Jang, Sang Ho Choi, Tae Sung Kim
Frontiers in Immunology.2018;[Epub] CrossRef
Influence of oxyR on Growth, Biofilm Formation, and Mobility of Vibrio parahaemolyticus
Chun-Hui Chung, Shin-yuan Fen, Shu-Chuan Yu, Hin-chung Wong, H. Nojiri
Applied and Environmental Microbiology.2016; 82(3): 788. CrossRef
Protective roles ofkatG-homologous genes against extrinsic peroxides inVibrio parahaemolyticus
Shu-Chuan Yu, Shin-yuan Fen, Cheng-Lun Chien, Hin-chung Wong, Séamus Fanning
FEMS Microbiology Letters.2016; 363(6): fnw038. CrossRef
Distinct characteristics of OxyR2, a new OxyR‐type regulator, ensuring expression of Peroxiredoxin 2 detoxifying low levels of hydrogen peroxide in Vibrio vulnificus
Suyeon Kim, Ye‐Ji Bang, Dukyun Kim, Jong Gyu Lim, Man Hwan Oh, Sang Ho Choi
Molecular Microbiology.2014; 93(5): 992. CrossRef
Characterization of the Vibrio vulnificus 1-Cys Peroxiredoxin Prx3 and Regulation of Its Expression by the Fe-S Cluster Regulator IscR in Response to Oxidative Stress and Iron Starvation
Jong Gyu Lim, Ye-Ji Bang, Sang Ho Choi
Journal of Biological Chemistry.2014; 289(52): 36263. CrossRef
IscR Is a Global Regulator Essential for Pathogenesis of Vibrio vulnificus and Induced by Host Cells
Jong Gyu Lim, Sang Ho Choi, A. Camilli
Infection and Immunity.2014; 82(2): 569. CrossRef
Activities of Alkyl Hydroperoxide Reductase Subunits C1 and C2 of Vibrio parahaemolyticus against Different Peroxides
Chun-Hui Chung, Tsung-yong Ma, Shin-yuan Fen, Hin-chung Wong, J. Björkroth
Applied and Environmental Microbiology.2014; 80(23): 7398. CrossRef
Roles of Alkyl Hydroperoxide Reductase Subunit C (AhpC) in Viable but Nonculturable Vibrio parahaemolyticus
Hen-Wei Wang, Chun-Hui Chung, Tsung-Yong Ma, Hin-chung Wong
Applied and Environmental Microbiology.2013; 79(12): 3734. CrossRef
Distinct Characteristics of Two 2-Cys Peroxiredoxins of Vibrio vulnificus Suggesting Differential Roles in Detoxifying Oxidative Stress
Ye-Ji Bang, Man Hwan Oh, Sang Ho Choi
Journal of Biological Chemistry.2012; 287(51): 42516. CrossRef
Characterizing the Host and Symbiont Proteomes in the Association between the Bobtail Squid, Euprymna scolopes, and the Bacterium, Vibrio fischeri
Tyler R. Schleicher, Spencer V. Nyholm, Immo A. Hansen
PLoS ONE.2011; 6(10): e25649. CrossRef
Evidence that Vibrio vulnificus ahpC2 is essential for survival under high salinity by modulating intracellular level of ROS
Myung Jin Koh, Hyun Sung Lee, Jee Eun Rhee, Sang Ho Choi
The Journal of Microbiology.2010; 48(1): 129. CrossRef

First
Prev
Page of 2
Next
Last

Previous issues