Journal of Microbiology

Volume 54(6); June 2016

Review

MINIREVIEW] Advances in novel influenza vaccines: a patent review: Jae-Min Song; J. Microbiol. 2016;54(6):403-412. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6176-7

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The threat of a major human influenza pandemic such as the avian H5N1 or the 2009 new H1N1 has emphasized the need for effective prevention strategies to combat these pathogens. Although egg based influenza vaccines have been well established for a long time, it remains an ongoing public health need to develop alternative production methods that ensures improved safety, efficacy, and ease of administration compared with conventional influenza vaccines. This article is intended to cover some of the recent advances and related patents on the development of influenza vaccines including live attenuated, cell based, genomic and synthetic peptide vaccines.

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Plant-made virus-like particles bearing influenza hemagglutinin (HA) recapitulate early interactions of native influenza virions with human monocytes/macrophages
Alexander I. Makarkov, Sabrina Chierzi, Stéphane Pillet, Keith K. Murai, Nathalie Landry, Brian J. Ward
Vaccine.2017; 35(35): 4629. CrossRef

Journal Articles

Calculibacillus koreensis gen. nov., sp. nov., an anaerobic Fe(III)-reducing bacterium isolated from sediment of mine tailings: Ui-Gi Min , So-Jeong Kim , Heeji Hong , Song-Gun Kim , Joo-Han Gwak , Man-Young Jung , Jong-Geol Kim , Jeong-Geol Na , Sung-Keun Rhee; J. Microbiol. 2016;54(6):413-419. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6086-8

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Abstract

A strictly anaerobic bacterium, strain B5T, was isolated from sediment of an abandoned coal mine in Taebaek, Republic of Korea. Cells of strain B5T were non-spore-forming, straight, Gram-positive rods. The optimum pH and temperature for growth were pH 7.0 and 30°C, respectively, while the strain was able to grow within pH and temperature ranges of 5.5– 7.5 and 25–45°C, respectively. Growth of strain B5T was observed at NaCl concentrations of 0 to 6.0% (w/v) with an optimum at 3.0–4.0% (w/v). The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and three unknown polar lipids. Strain B5T grew anaerobically by reducing nitrate, nitrite, ferric-citrate, ferric-nitrilotriacetate, elemental sulfur, thiosulfate, and anthraquinone- 2-sulfonate in the presence of proteinaceous compounds, organic acids, and carbohydrates as electron donors. The isolate was not able to grow by fermentation. Strain B5T did not grow under aerobic or microaerobic conditions. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B5T is most closely related to the genus Tepidibacillus (T. fermentans STGHT; 96.3%) and Vulcanibacillus (V. modesticaldus BRT; 94.6%). The genomic DNA G+C content (36.9 mol%) of strain B5T was higher than those of T. fermentans STGHT (34.8 mol%) and V. modesticaldus BRT (34.5 mol%). Based on its phenotypic, chemotaxonomic, and phylogenetic properties, we describe a new species of a novel genus Calculibacillus, represented by strain B5T (=KCTC 15397T =JCM 19989T), for which we propose the name Calculibacillus koreensis gen. nov., sp. nov.

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Bacillales: From Taxonomy to Biotechnological and Industrial Perspectives
Sharareh Harirchi, Taner Sar, Mohaddaseh Ramezani, Habibu Aliyu, Zahra Etemadifar, Seyed Ali Nojoumi, Fatemeh Yazdian, Mukesh Kumar Awasthi, Mohammad J. Taherzadeh
Microorganisms.2022; 10(12): 2355. CrossRef
List of new names and new combinations that have appeared in effective publications outside of the IJSEM and are submitted for valid publication
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology .2019;[Epub] CrossRef
Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea
Yeon Bee Kim, Joon Yong Kim, Hye Seon Song, Changsu Lee, Joseph Kwon, Jisu Kang, Jin-Kyu Rhee, Myeong Seon Jeong, Young-Do Nam, Seong Woon Roh
Gut Pathogens.2017;[Epub] CrossRef

Dankookia rubra gen. nov., sp. nov., an alphaproteobacterium isolated from sediment of a shallow stream: Wan-Hoe Kim , Do-hak Kim , Keunsoo Kang , Tae-Young Ahn; J. Microbiol. 2016;54(6):420-425. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6054-3

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Abstract

WS-10T–a Gram-negative, non-motile, and aerobic bacterial strain–was isolated from the sediment of a shallow stream in Korea. The optimum ranges of temperature and pH for growth were 20–40°C (optimum 28°C) and pH 6.0–8.0 (optimum pH 7.0), respectively. The DNA G+C content of strain WS-10T was 72.7 mol%. The major fatty acids (>5%) were summed feature 8 (C18:1 ω7c), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0, and C18:1 2-OH. The major polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, and unidentified aminolipids. Q-10 was the predominant respiratory quinone. The highest similarities in the 16S rRNA gene sequence were shown with Paracraurococcus ruber (95.3%), Belnapia soli (95.3%), B. moabensis (95.1%), and B. rosea (95.0%). A phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain WS-10T formed a distinct line within a clade containing the genera Paracraurococcus, Craurococcus, and Belnapia in the family Acetobacteraceae. On the basis of polyphasic evidence, strain WS-10T represents a novel species of a new genus in the family Acetobacteraceae, for which the name Dankookia rubra gen. nov., sp. nov. is proposed. The type strain of the type species is WS-10T (= KACC 18533T = JCM 30602T).

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Limobrevibacterium gyesilva gen. nov., sp. nov., Isolated from Forest Soil
Nhan Le Thi Tuyet, Jaisoo Kim
Diversity.2023; 15(5): 684. CrossRef
Roseicella aquatilis sp. nov., isolated from freshwater lake
Dan-Dan Zhang, Ya-Nan Zhao, Rui-Han He, Yu-Qi Yan, Zong-Jun Du
Archives of Microbiology.2022;[Epub] CrossRef
Sabulicella rubraurantiaca gen. nov., sp. nov., a new member of the family Acetobacteraceae, isolated from desert soil
Zhi-Liang Lin, Shuai Li, Qi-Chuang Wei, Wen-Hui Lian, Chun-Yan Lu, Lu Xu, Li-Nan Huang, Lei Dong, Wen-Jun Li
Archives of Microbiology.2022;[Epub] CrossRef
Caldovatus aquaticus sp. nov., a moderately thermophilic bacterium isolated from hot spring microbial mat
Chao-Jian Hu, Wen-Dong Xian, Yi-Qing Lv, Cheng-Xiang Peng, Rong-Xu Shan, Ze-Chang Cheng, Qian Lv, Ye Tian, Jian-Yu Jiao, Sha Tan, Wen-Jun Li
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera
Anusha Rai, Uppada Jagadeeshwari, Gupta Deepshikha, Nandardhane Smita, Chintalapati Sasikala, Chintalapati Venkata Ramana
Frontiers in Microbiology.2021;[Epub] CrossRef
Roseicella frigidaeris gen. nov., sp. nov., isolated from an air-conditioning system
Shehzad Abid Khan, Sang Eun Jeong, Hye Su Jung, Zhe-Xue Quan, Che Ok Jeon
International Journal of Systematic and Evolutionary Microbiology.2019; 69(5): 1384. CrossRef
Isolation of Uncultured Bacteria from Antarctica Using Long Incubation Periods and Low Nutritional Media
Andre A. Pulschen, Amanda G. Bendia, Ashwana D. Fricker, Vivian H. Pellizari, Douglas Galante, Fabio Rodrigues
Frontiers in Microbiology.2017;[Epub] CrossRef
Siccirubricoccus deserti gen. nov., sp. nov., a proteobacterium isolated from a desert sample
Zi-Wen Yang, Nimaichand Salam, Zheng-Shuang Hua, Bing-Bing Liu, Ming-Xian Han, Bao-Zhu Fang, Dong Wang, Min Xiao, Wael N. Hozzein, Wen-Jun Li
International Journal of Systematic and Evolutionary Microbiology.2017; 67(11): 4862. CrossRef
List of new names and new combinations previously effectively, but not validly, published
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology .2016; 66(9): 3761. CrossRef

PprM is necessary for up-regulation of katE1, encoding the major catalase of Deinococcus radiodurans, under unstressed culture conditions: Sun-Wook Jeong , Ho Seong Seo , Min-Kyu Kim , Jong-Il Choi , Heon-Man Lim , Sangyong Lim; J. Microbiol. 2016;54(6):426-431. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6175-8

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Abstract

Deinococcus radiodurans is a poly-extremophilic organism, capable of tolerating a wide variety of different stresses, such as gamma/ultraviolet radiation, desiccation, and oxidative stress. PprM, a cold shock protein homolog, is involved in the radiation resistance of D. radiodurans, but its role in the oxidative stress response has not been investigated. In this study, we investigated the effect of pprM mutation on catalase gene expression. pprM disruption decreased the mRNA and protein levels of KatE1, which is the major catalase in D. radiodurans, under normal culture conditions. A pprM mutant strain (pprMMT) exhibited decreased catalase activity, and its resistance to hydrogen peroxide (H2O2) decreased accordingly compared with that of the wild-type strain. We confirmed that RecG helicase negatively regulates katE1 under normal culture conditions. Among katE1 transcriptional regulators, the positive regulator drRRA was not altered in pprM-, while the negative regulators perR, dtxR, and recG were activated more than 2.5-fold in pprMMT. These findings suggest that PprM is necessary for KatE1 production under normal culture conditions by down-regulation of katE1 negative regulators.

Citations

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Antioxidant defense of Deinococcus radiodurans : how does it contribute to extreme radiation resistance?
Izabela Sadowska-Bartosz, Grzegorz Bartosz
International Journal of Radiation Biology.2023; 99(12): 1803. CrossRef
Development and Regulation of the Extreme Biofilm Formation of Deinococcus radiodurans R1 under Extreme Environmental Conditions
Qiannan Guo, Yuhua Zhan, Wei Zhang, Jin Wang, Yongliang Yan, Wenxiu Wang, Min Lin
International Journal of Molecular Sciences.2023; 25(1): 421. CrossRef
A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
Jordan K. Villa, Runhua Han, Chen-Hsun Tsai, Angela Chen, Philip Sweet, Gabriela Franco, Respina Vaezian, Rok Tkavc, Michael J. Daly, Lydia M. Contreras
Scientific Reports.2021;[Epub] CrossRef
Lack of the Bacterial Phytochrome Protein Decreases Deinococcus radiodurans Resistance to Mitomycin C
Jong-Hyun Jung, Soyoung Jeong, Seonghun Im, Min-Kyu Kim, Ho Seong Seo, Sangyong Lim
Frontiers in Microbiology.2021;[Epub] CrossRef
Effects of Conserved Wedge Domain Residues on DNA Binding Activity of Deinococcus radiodurans RecG Helicase
Sun-Wook Jeong, Min-Kyu Kim, Lei Zhao, Seul-Ki Yang, Jong-Hyun Jung, Heon-Man Lim, Sangyong Lim
Frontiers in Genetics.2021;[Epub] CrossRef
The Novel ncRNA OsiR Positively Regulates Expression of katE2 and is Required for Oxidative Stress Tolerance in Deinococcus radiodurans
Lihua Gao, Xiaonan Chen, Ye Tian, Yongliang Yan, Yuhua Zhan, Zhengfu Zhou, Wei Zhang, Min Lin, Ming Chen
International Journal of Molecular Sciences.2020; 21(9): 3200. CrossRef
Conservation and diversity of radiation and oxidative stress resistance mechanisms inDeinococcusspecies
Sangyong Lim, Jong-Hyun Jung, Laurence Blanchard, Arjan de Groot
FEMS Microbiology Reviews.2019; 43(1): 19. CrossRef
Gene regulation for the extreme resistance to ionizing radiation of Deinococcus radiodurans
Wuzhou Wang, Yun Ma, Junyan He, Huizhou Qi, Fangzhu Xiao, Shuya He
Gene.2019; 715: 144008. CrossRef
PprM, a Cold Shock Domain-Containing Protein from Deinococcus radiodurans, Confers Oxidative Stress Tolerance to Escherichia coli
Sun-Ha Park, Harinder Singh, Deepti Appukuttan, Sunwook Jeong, Yong Jun Choi, Jong-Hyun Jung, Issay Narumi, Sangyong Lim
Frontiers in Microbiology.2017;[Epub] CrossRef
Knockout of pprM Decreases Resistance to Desiccation and Oxidation in Deinococcus radiodurans
Yang Zeng, Yun Ma, Fangzhu Xiao, Wuzhou Wang, Shuya He
Indian Journal of Microbiology.2017; 57(3): 316. CrossRef
RNA-Binding Domain is Necessary for PprM Function in Response to the Extreme Environmental Stress in Deinococcus radiodurans
Wei Li, Yun Ma, Jie Yang, Fangzhu Xiao, Wuzhou Wang, Shuya He
Indian Journal of Microbiology.2017; 57(4): 492. CrossRef

Identification of D-amino acid dehydrogenase as an upstream regulator of the autoinduction of a putative acyltransferase in Corynebacterium glutamicum: Jung-Hoon Lee , Yong-Jae Kim , Hee-Sung Shin , Heung-Shick Lee , Shouguang Jin , Un-Hwan Ha; J. Microbiol. 2016;54(6):432-439. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6046-3

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Abstract

Expression of a putative acyltransferase encoded by NCgl- 0350 of Corynebacterium glutamicum is induced by cell-free culture fluids obtained from stationary-phase growth of both C. glutamicum and Pseudomonas aeruginosa, providing evidence for interspecies communication. Here, we further confirmed that such communication occurs by showing that acyltransferase expression is induced by culture fluid obtained from diverse Gram-negative and -positive bacterial strains, including Escherichia coli, Salmonella Typhimurium, Bacillus subtilis, Staphylococcus aureus, Mycobacterium sp. strain JC1, and Mycobacterium smegmatis. A homologous acyltransferase encoded by PA5238 of P. aeruginosa was also induced by fluids obtained from P. aeruginosa as well as other bacterial strains, as observed for NCgl0350 of C. glutamicum. Because C. glutamicum is difficult to study using molecular approaches, the homologous gene PA5238 of P. aeruginosa was used to identify PA5309 as an upstream regulator of expression. A homologous D-amino acid dehydrogenase encoded by NCgl- 2909 of C. glutamicum was cloned based on amino acid similarity to PA5309, and its role in the regulation of NCgl0350 expression was confirmed. Moreover, NCgl2909 played positive roles in growth of C. glutamicum. Thus, we identified a D-amino acid dehydrogenase as an upstream regulator of the autoinduction of a putative acyltransferase in C. glutamicum.

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Enhanced Bacterial Growth and Gene Expression of D-Amino Acid Dehydrogenase With D-Glutamate as the Sole Carbon Source
Takeshi Naganuma, Yoshiakira Iinuma, Hitomi Nishiwaki, Ryota Murase, Kazuo Masaki, Ryosuke Nakai
Frontiers in Microbiology.2018;[Epub] CrossRef

Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans: Young Jun An , Sara E. Rowland , Frank T. Robb , Sun-Shin Cha; J. Microbiol. 2016;54(6):440-444. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6089-5

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Abstract

Chaperonins (CPNs) are megadalton sized ATP-dependent nanomachines that facilitate protein folding through complex cycles of complex allosteric articulation. They consist of two back-to-back stacked multisubunit rings. CPNs are usually classified into Group I and Group II. Here, we report the crystallization of both the AMPPNP (an ATP analogue) and ADP bound forms of a novel CPN, classified as belonging to a third Group, recently discovered in the extreme thermophile Carboxydothermus hydrogenoformans. Crystals of the two forms were grown by the vapor batch crystallization
method
at 295 K. Crystals of the Ch-CPN/AMPPNP complex diffracted to 3.0 Å resolution and belonged to the space group P422, with unit-cell parameters a = b = 186.166, c = 160.742 Å. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 60.02%. Crystals of the Ch-CPN/ADP complex diffracted to 4.0 Å resolution and belonged to the space group P4212, with unit-cell parameters a = b = 209.780, c = 169.813Å. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 70.19%.

Citations

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Bridging human chaperonopathies and microbial chaperonins
Everly Conway de Macario, Masafumi Yohda, Alberto J. L. Macario, Frank T. Robb
Communications Biology.2019;[Epub] CrossRef
Prokaryotic Chaperonins as Experimental Models for Elucidating Structure-Function Abnormalities of Human Pathogenic Mutant Counterparts
Everly Conway de Macario, Frank T. Robb, Alberto J. L. Macario
Frontiers in Molecular Biosciences.2017;[Epub] CrossRef
Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Young Jun An, Sara E. Rowland, Jung-Hyun Na, Dario Spigolon, Seung Kon Hong, Yeo Joon Yoon, Jung-Hyun Lee, Frank T. Robb, Sun-Shin Cha
Nature Communications.2017;[Epub] CrossRef

Characteristics of the community-genotype sequence type 72 methicillin-resistant Staphylococcus aureus isolates that underlie their persistence in hospitals: Eun-Jeong Joo , Ji-Young Choi , Doo Ryeon Chung , Jae-Hoon Song , Kwan Soo Ko; J. Microbiol. 2016;54(6):445-450. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6157-x

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Abstract

Panton-Valentine leukocidin-negative methicillin-resistant Staphylococcus aureus (MRSA) clone ST72, known as a major community-associated MRSA in Korea, has emerged as an important pathogen in hospitals. To understand bacterial properties that underlie transformation of this clone into a nosocomial pathogen, we compared characteristics of the community-genotype ST72 MRSA isolates with those of ST5 and ST239 MRSA, which have been predominant nosocomial MRSA clones in Korea. Several genes associated with adhesion and virulence were absent or rarely found in ST72 isolates. Many ST72 isolates (70.1%) belonged to agr group I, but the agr group of other ST72 isolates could not be determined. As indicated by δ-hemolysin production, ST72 isolates expressed fully functional agr, whereas agr dysfunction was observed in ST5 and ST239 isolates. In the biofilm formation assay, no upregulation of biofilm-forming activity of ST72 MRSA was detected. However, ST72 isolates demonstrated persistence under hypotonic and desiccating conditions (survival rates 72.3% and 33.9%, respectively), which was similar to characteristics of ST5 or ST239 isolates. ST72- MRSA isolates showed low virulence, but properties of their functional agr system could facilitate their spread in hospitals. In conclusion, tolerance to stressful environments, e.g., hypotonic and dry conditions, may also contribute to survival of the community-associated MRSA clones in healthcare facilities.

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Dynamics of the MRSA Population in a Chilean Hospital: a Phylogenomic Analysis (2000–2016)
José R. W. Martínez, Paul J. Planet, Maria Spencer-Sandino, Lina Rivas, Lorena Díaz, Ahmed M. Moustafa, Ana Quesille-Villalobos, Roberto Riquelme-Neira, Manuel Alcalde-Rico, Blake Hanson, Lina P. Carvajal, Sandra Rincón, Jinnethe Reyes, Marusella Lam, Jua
Microbiology Spectrum.2023;[Epub] CrossRef
Comparing the Phylogenetic Distribution of Multilocus Sequence Typing, Staphylococcal Protein A, and Staphylococcal Cassette Chromosome Mec Types in Methicillin-Resistant Staphylococcus Aureus (MRSA) in Korea from 1994 to 2020
You-Jin Hwang
Antibiotics.2023; 12(9): 1397. CrossRef
Performance Evaluation of the IR Biotyper® System for Clinical Microbiology: Application for Detection of Staphylococcus aureus Sequence Type 8 Strains
Jun Sung Hong, Dokyun Kim, Seok Hoon Jeong
Antibiotics.2022; 11(7): 909. CrossRef
Comparative Genomic Analysis Provides Insights into the Evolution and Genetic Diversity of Community-Genotype Sequence Type 72 Staphylococcus aureus Isolates
Wangxiao Zhou, Ye Jin, Yanzi Zhou, Yuan Wang, Luying Xiong, Qixia Luo, Yonghong Xiao, Suleyman Yildirim
mSystems.2021;[Epub] CrossRef
C-reactive protein predicts persistent bacteremia caused by community-acquired methicillin-resistant Staphylococcus aureus strain
Hyemin Chung, Eunsil Kim, Eunmi Yang, Yun Woo Lee, Joung Ha Park, Seongman Bae, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim
European Journal of Clinical Microbiology & Infectious Diseases.2021; 40(12): 2497. CrossRef
Genome-Wide Analysis of Staphylococcus aureus Sequence Type 72 Isolates Provides Insights Into Resistance Against Antimicrobial Agents and Virulence Potential
Nayab Batool, Amen Shamim, Akhilesh Kumar Chaurasia, Kyeong Kyu Kim
Frontiers in Microbiology.2021;[Epub] CrossRef
A Longitudinal Study of Adult Patients withStaphylococcus aureusBacteremia over 11 Years in Korea
Seong-Ho Choi, Jeongsoo Lee, Jiwon Jung, Eun Sil Kim, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Jun Hee Woo, Yang Soo Kim
Journal of Korean Medical Science.2021;[Epub] CrossRef
Changing characteristics of S. aureus bacteremia caused by PVL-negative, MRSA strain over 11 years
Eunmi Yang, Eunsil Kim, Hyemin Chung, Yun Woo Lee, Seongman Bae, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Ho Choi, Sang-Oh Lee, Yang Soo Kim
Scientific Reports.2021;[Epub] CrossRef
Functional Identification of Serine Hydroxymethyltransferase as a Key Gene Involved in Lysostaphin Resistance and Virulence Potential of Staphylococcus aureus Strains
Nayab Batool, Kwan Soo Ko, Akhilesh Kumar Chaurasia, Kyeong Kyu Kim
International Journal of Molecular Sciences.2020; 21(23): 9135. CrossRef
Sterilization efficiency of pathogen-contaminated cottons in a laundry machine
Yoonjae Shin, Jungha Park, Woojun Park
Journal of Microbiology.2020; 58(1): 30. CrossRef
Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
Lorena Tuchscherr, Christine Pöllath, Anke Siegmund, Stefanie Deinhardt-Emmer, Verena Hoerr, Carl-Magnus Svensson, Marc Thilo Figge, Stefan Monecke, Bettina Löffler
Toxins.2019; 11(3): 135. CrossRef
Impact of Community-Onset Methicillin-ResistantStaphylococcus aureusonStaphylococcus aureusBacteremia in a Central Korea Veterans Health Service Hospital
Eunsin Bae, Choon Kwan Kim, Jung-Hyun Jang, Heungsup Sung, YounMi Choi, Mi-Na Kim
Annals of Laboratory Medicine.2019; 39(2): 158. CrossRef
Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean
Yae Sung Mun, You Jin Hwang
Antibiotics.2019; 8(4): 202. CrossRef
Emergence of Community-Genotype Methicillin-ResistantStaphylococcus aureusin Korean Hospitals: Clinical Characteristics of Nosocomial Infections by Community-Genotype Strain
Eun-Jeong Joo, Doo Ryeon Chung, So Hyun Kim, Jin Yang Baek, Nam Yong Lee, Sun Young Cho, Young Eun Ha, Cheol-In Kang, Kyong Ran Peck, Jae-Hoon Song
Infection & Chemotherapy.2017; 49(2): 109. CrossRef
Comparison of subsequent infection in methicillin-resistant Staphylococcus aureus nasal carriers between ST72 community-genotype and hospital genotypes: a retrospective cohort study
So Yeon Park, Doo Ryeon Chung, Yu Ri Kang, So Hyun Kim, Sun Young Cho, Young Eun Ha, Cheol-In Kang, Kyong Ran Peck, Jae-Hoon Song
Antimicrobial Resistance & Infection Control.2017;[Epub] CrossRef
Molecular Typing and Resistance Profiles of Vancomycin-IntermediateStaphylococcus aureusin Korea: Results from a National Surveillance Study, 2007-2013
Jung Wook Kim, Gi Su Kang, Jae Il Yoo, Hwa Su Kim, Yeong Seon Lee, Jae-Yon Yu, Kwang-Jun Lee, Chan Park, Il-Hwan Kim
Annals of Clinical Microbiology.2016; 19(4): 88. CrossRef

Roles of human apolipoprotein E in the infectivity and replication of hepatitis C virus genotype 2a: Bo-Kyoung Jung , Hye-Ran Kim , Gyu-Nam Park , Guangxiang Luo , Kyung-Soo Chang; J. Microbiol. 2016;54(6):451-458. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6099-3

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Abstract

Hepatitis C virus (HCV) infection is associated with lipoproteins, and apolipoprotein E (apoE) plays an essential role in infectious HCV particles. Although the role of apoE in HCV infection is well known, its role in the replication of HCV remains unclear. The aims of this study were to determine the role of apoE in the RNA replication of major HCV genotypes 1b and 2a, and to determine whether this role is HCVgenotype- dependent using HCV genotype 1b replicon cells and HCV genotype 2a producing (HP) cells. HCV infection was blocked in Huh7.5 cells treated with low-density lipoproteins, very low-density lipoproteins, or apoE3. An apoE3- specific monoclonal antibody also efficiently neutralized HCV infectivity, and HCV infection was dramatically suppressed by the knockdown of apoE expression with an apoE-specific small interfering RNA, suggesting a requirement for apoE in infectious HCV particles. HCV RNA replication was not affected in HP cells treated with each apoE isoform or transfected with apoE-specific siRNAs. However, the knockdown of apoE expression suppressed RNA replication of HCV genotype 1b. The siRNA-mediated knockdown of apoE, apoA1, and apoB expression also suppressed the RNA replication of HCV genotype 1b, but not that of HCV genotype 2a. Taken together, these findings indicate that apoE plays an important role in HCV genotype 2a infection and in HCV genotype 1b RNA replication, but not in the replication of HCV genotype 2a. These results provide important information for the future development of HCV-genotypespecific anti-HCV agents.

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The Role of ApoE in HCV Infection and Comorbidity
Yue Gong, Wei Cun
International Journal of Molecular Sciences.2019; 20(8): 2037. CrossRef
How Have Retrovirus Pseudotypes Contributed to Our Understanding of Viral Entry?
Barnabas King, Alexander W Tarr
Future Virology.2017; 12(10): 569. CrossRef

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