Journal of Microbiology

Research Support, Non-U.S. Gov't

Effect of Mycelial Extract of Clavicorona pyxidata on the Production of Amyloid β-Peptide and the Inhibition of Endogenous β-Secretase Activity in vitro: Tae-Hee Lee , Young-Il Park , Yeong-Hwan Han; J. Microbiol. 2006;44(6):665-670.; DOI: https://doi.org/2459 [pii]

Abstract: Amyloid β-peptide (Aβ), which is a product of the proteolytic effect of β-secretase (BACE) on an amyloid precursor protein, is closely associated with Alzheimer’s disease (AD) pathogenesis. There is sufficient evidence to suggest that a BACE inhibitor may reduce Aβ levels, thus decreasing the risk of AD. In a previous study, an extract of Clavicorona pyxidata DGUM 29005 mycelia was found to inhibit the production of a soluble β-amyloid precursor protein (sβAPP), Aβ, and BACE in neuronal cell lines. We sought to determine whether this mycelial extract exerts the same effect in human rhabdomyosarcoma A-204 and rat pheochromocytoma PC-12 cells. We found that the production of Aβ decreased in a dose-dependent manner in the presence of the mycelial extract and that the concentration of Aβ never exceeded 50 μg/ml. The presence of sAPP was detected in every culture medium to which the mycelial extract had been added and its concentration remained the same, regardless of the concentration of the extract used. Endogenous β-secretase <br>activity in A-204 and PC-12 cellular homogenates also decreased in the presence of this extract. These cells, in culture, were not susceptible to the cytotoxic activity of the mycelial extract.

Journal Article

Effect of Mycelial Extract of Clavicorona pyxidata on Acetylcholinesterase and β-Secretase Activity in vitro: Tae-Hee Lee , Young-Il Park , Yeong-Hwan Han; J. Microbiol. 2006;44(5):502-507.; DOI: https://doi.org/2448 [pii]

Abstract: In a previous study, an extract of Clavicorona pyxidata DGUM 29005 mycelia demonstrated an inhibitory effect against enzyme-associated perceptual disorders. We have attempted to determine whether this mycelial extract is also capable of inhibiting the activities of acetylcholinesterase (AChE) and β-secretase (BACE) activity. Butanol, ethanol, and water extracts of C. pyxidata DGUM 29005 mycelia were shown to inhibit AChE activity by 99.3%, 93.7%, and 91.7%, respectively. The inhibitory value of the butanol extract was more profound than that of tacrine (95.4%). The ethanol extract also exerted an inhibitory effect against BACE activity; this fraction may harbor the potential for development into a pharmocotherapeutic modality for the treatment of Alzheimer''s disease (AD) patients. Rat pheochromocytoma PC12 cells in culture were not determined to be susceptible to the cytotoxic activity evidenced by the mycelial extract. The ethanol extract inhibited endogenous AChE activity in PC12 cellular homogenates, with an IC50 of 67.5 μg/ml, after incubation with intact cells, and also inhibited BACE activity in a dose-dependent fashion. These results suggest that the C. pyxidata mycelial extract has the potential to enhance cholinergic function and, therefore, may perform a function in the amelioration of the cholinergic deficit observed in cases of AD, as well as other types of age-associated memory impairment.

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