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- Isolation and Functional Characterization of a Delta 6-Desaturase Gene from the Pike Eel (Muraenesox cinereus)
- Sun Hee Kim , Kyung Hee Roh , Jung-Bong Kim , Kwang-Soo Kim , Nam Shin Kim , Hyun Uk Kim , Kyeong-Ryeol Lee , Jong-Sug Park , Jong-Bum Kim
- J. Microbiol. 2013;51(6):807-813. Published online October 5, 2013
- DOI: https://doi.org/10.1007/s12275-013-3144-3
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Abstract
-
Stearidonic acid (STA; 18:4n-3) and γ-linolenic acid (GLA;
18:3n-6) are significant intermediates in the biosynthetic pathway
for the very-long-chain polyunsaturated fatty acids of
eicosapentaenoic acid (EPA; 20:5n-3) and arachidonic acid
(ARA; 20:4n-6), respectively. To develop a sustainable system
for the production of dietary polyunsaturated fatty acids,
we focused on the action of the enzyme delta 6-desaturase
(D6DES) on the essential acids, linoleic acid (LA; 18:2n-6)
and α-linolenic acid (ALA; 18:3n-3). A 1,335-bp full-length
cDNA encoding D6DES (McD6DES) was cloned from Muraenesox
cinereus using degenerate PCR and RACE-PCR
methods
. To investigate the enzymatic activity of McD6DES in the production of n-6 and n-3 fatty acids, a recombinant plasmid expressing McD6DES (pYES-McD6DES) was transformed into and expressed in Saccharomyces cerevisiae. The exogenously expressed McD6DES produced GLA and STA at conversion rates of 14.2% and 45.9%, respectively, from the exogenous LA and ALA substrates. These results indicate that McD6DES is essentially a delta 6-desaturase involved in very-long-chain polyunsaturated fatty acid synthesis. -
Citations
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- Regulation of long-chain polyunsaturated fatty acid biosynthesis in teleost fish
Dizhi Xie, Cuiying Chen, Yewei Dong, Cuihong You, Shuqi Wang, Óscar Monroig, Douglas R. Tocher, Yuanyou Li
Progress in Lipid Research.2021; 82: 101095. CrossRef - Comparative and functional analysis of desaturase FADS1 (∆5) and FADS2 (∆6) orthologues of marine organisms
Crisalejandra Rivera-Pérez, Fausto Valenzuela-Quiñonez, Javier Caraveo-Patiño
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics.2020; 35: 100704. CrossRef - Δ6 fatty acid desaturases in polyunsaturated fatty acid biosynthesis: insights into the evolution, function with substrate specificities and biotechnological use
Jie Cui, Haiqin Chen, Xin Tang, Jianxin Zhao, Hao Zhang, Yong Q. Chen, Wei Chen
Applied Microbiology and Biotechnology.2020; 104(23): 9947. CrossRef - In Silico Structural Studies and Molecular Docking Analysis of Delta6-desaturase in HUFA Biosynthetic Pathway
Suvra Roy, Hirak jyoti Chakraborty, Vikash Kumar, B K Behera, R S Rana, Gireesh Babu
Animal Biotechnology.2018; 29(3): 161. CrossRef - Changes in Plasma and Tissue Long-Chain Polyunsaturated Fatty Acid (LC-PUFA) Content in the Eel Anguilla japonica After External and Internal Osmotic Stress
Qinghao Zhang, Marty K. S. Wong, Yiqi Li, Yuanyou Li, Yoshio Takei
Zoological Science.2017; 34(5): 429. CrossRef - Effect of low temperature on highly unsaturated fatty acid biosynthesis in activated sludge
Su He, Li-li Ding, Ke Xu, Jin-ju Geng, Hong-qiang Ren
Bioresource Technology.2016; 211: 494. CrossRef - Metabolic engineering to produce γ-linolenic acid in Brassica napus using a Δ6-desaturase from pike eel
Sun Hee Kim, Kyung Hee Roh, Kyeong-Ryeol Lee, Han-Chul Kang, Hyun Uk Kim, Jong Bum Kim
Plant Biotechnology Reports.2016; 10(6): 475. CrossRef - Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids inArabidopsis
Sun Hee Kim, Kyung Hee Roh, Jong-Sug Park, Kwang-Soo Kim, Hyun Uk Kim, Kyeong-Ryeol Lee, Han-Chul Kang, Jong-Bum Kim
BioMed Research International.2015; 2015: 1. CrossRef - Coexpression of multiple genes reconstitutes two pathways of very long-chain polyunsaturated fatty acid biosynthesis in Pichia pastoris
Sun Hee Kim, Kyung Hee Roh, Kwang-Soo Kim, Hyun Uk Kim, Kyeong-Ryeol Lee, Han-Chul Kang, Jong-Bum Kim
Biotechnology Letters.2014; 36(9): 1843. CrossRef
- Regulation of long-chain polyunsaturated fatty acid biosynthesis in teleost fish
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