Journal of Microbiology

Research Support, Non-U.S. Gov'ts

Characterization of Conjugative Plasmids Carrying Antibiotic Resistance Genes Encoding 16S rRNA Methylase, Extended-Spectrum Beta-Lactamase, and/or Plasmid-Mediated AmpC Beta-Lactamase: Hee Young Kang , Jungmin Kim , Sung Yong Seol , Yoo Chul Lee , Je Chul Lee , Dong Taek Cho; J. Microbiol. 2009;47(1):68-75. Published online February 20, 2009; DOI: https://doi.org/10.1007/s12275-008-0158-3

Abstract: In this study, we identified extended-spectrum ß-lactamase (ESBL) and plasmid-mediated AmpC ß-lactamase which were associated with 16S rRNA methylase gene on the conjugative plasmid. Among 82 clinical isolates of Enterobacteriaceae that carry 16S rRNA methylase gene (64 strains, armA, and 18 strains, rmtB), blaSHV-12 was detected either alone or combined with blaDHA-1, blaCTX-M-3, and blaCTX-M-14 in 30 strains carrying armA and 6 strains carrying rmtB. The blaCTX-M-3 was detected in 13 of 64 strains carrying armA but no strains carrying rmtB. Whereas blaCTX-M-14 was detected in 15 of 18 strains carrying rmtB but only 2 of 64 strains carrying armA. Overall, blaSHV-12 and blaCTX-M-14 was the most common ESBL gene which was associated with armA and rmtB, respectively. In addition, we found that blaCTX-M-3 localized with armA on the same IncL/M plasmid and blaCTX-M-14 localized with rmtB on the same IncA/C plasmid. Restriction fragment length polymorphism of conjugative plasmids and pulsed-field gel electrophoresis of genomic DNAs revealed that intercellular horizontal transfer of conjugative plasmid and clonal transmission have been occurred at the same time.

Multidrug-Resistant Providencia Isolates Carrying blaPER-1, blaVIM-2, and armA: Hee-Woo Lee , Hee-Young Kang , Kyeong-Seob Shin , Jungmin Kim; J. Microbiol. 2007;45(3):272-274.; DOI: https://doi.org/2531 [pii]

Abstract: During May to July 2004, three strains of Providencia spp. with multidrug-resistance (MDR) were isolated from urinary specimen of three patients hospitalized with a same hospital room. By PCR analysis, all three strains have been found to carry both VIM-2 type metallo-β-lactamase gene and PER-1 type extendedspectrum β-lactamase gene. One out of three strains carried additional resistance gene, armA, 16S rRNA methylase gene responsible for high level resistance to aminoglycosides. To our knowledge, this is the first report on the identification of Providencia spp. simultaneously carrying blaVIM-2, blaPER-1, and armA genes.

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