Journal of Microbiology

Full articles

Lactiplantibacillus koreensis sp. nov. and Lactiplantibacillus kimchii sp. nov., isolated from kimchi, a traditional Korean fermented food: Min Ji Lee, Jisu Lee, Sohee Nam, Mi-Ja Jung, Yeon Bee Kim, Yujin Kim, Jeong Ui Yun, Seong Woon Roh, Tae Woong Whon, Che Ok Jeon, Se Hee Lee; J. Microbiol. 2025;63(11):e2507007. Published online November 30, 2025; DOI: https://doi.org/10.71150/jm.2507007

1,057 View
38 Download

Abstract PDF Supplementary Material: Two Gram-stain-positive, facultatively anaerobic, rod-shaped, and non-motile lactic acid bacterial strains, designated as strains CBA3605^T and CBA3606^T, were isolated from kimchi, a traditional Korean fermented food. Both strains were oxidase- and catalase-negative, non-spore-forming, non-hemolytic, and non-gas-producing. Optimal growth conditions for the two strains were observed at 30°C, pH 5.0, and 0% NaCl. The two genomes were composed of a circular chromosome and three plasmids and the DNA G + C content of 43.0%, respectively. Strains CBA3605^T and CBA3606^T were most closely related to Lactiplantibacillus (Lp.) pingfangensis 382-1^T with 16S rRNA sequence similarity of 99.4% and 99.1%, respectively. However, the orthologous average nucleotide identities between CBA3605^T and CBA3606^T were 91.7%, and those with strain 382-1^T were 76.9% and 76.5%, respectively. Digital DNA–DNA hybridization values between CBA3605^T and CBA3606^T were 45.0%, and those with strain 382-1^T were 21.4% and 21.0%, respectively. The major fatty acids detected in both strains included C_16:0, C_18:1 ω9c, and summed features 7 (C_19:1 ω7c, C_19:1 ω6c, C_19:0 cyclo ω10c, and/or C_19:0 ω6c). The peptidoglycan of both strains CBA3605^T and CBA3606^T contained meso-diaminopimelic acid and was classified as A4α type (L-Lys–D-Asp). In polar lipid analyses, only strain CBA3605^T contained aminophosphoglycolipid, which was absent in CBA3606^T, although both strains harbored same major polar lipids (diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine). Based on phenotypic, phylogenetic, genomic, biochemical, and chemotaxonomic analyses, strains CBA3605^T and CBA3606^T represent two novel species of the genus Lactiplantibacillus, for which the names Lactiplantibacillus koreensis sp. nov. and Lactiplantibacillus kimchii sp. nov. are proposed, with CBA3605^T (= KACC 81073BP^T = JCM 37965^T), and CBA3606^T (= KACC 81074BP^T = JCM 37966^T) as the type strains.

Pycnogenol reduces the expression of P. aeruginosa T3SS and inflammatory response in NCI-H292 cells: Seung-Ho Kim, Da Yun Seo, Sang-Bae Han, Un-Hwan Ha, Ji-Won Park, Kyung-Seop Ahn; J. Microbiol. 2025;63(10):2503004. Published online September 19, 2025; DOI: https://doi.org/10.71150/jm.2503004

1,102 View
32 Download

Abstract PDF Supplementary Material: Nosocomial infections caused by Pseudomonas aeruginosa (P. aeruginosa) have become increasingly common, particularly among immunocompromised individuals, who experience high mortality rates and prolonged treatment durations due to the limited availability of effective therapies. In this study, we screened for anti-ExoS compounds targeting P. aeruginosa and identified pycnogenol (PYC) as a potent inhibitor of the type III secretion system (T3SS), a major virulence mechanism responsible for the translocation of effectors such as ExoS. Using ELISA, western blotting, and real-time PCR analyses in both P. aeruginosa and infected H292 cells, we found that PYC significantly reduced T3SS activity. Mechanistically, PYC suppressed the transcription of T3SS-related genes by downregulating exsA expression in P. aeruginosa. Furthermore, pretreatment with PYC attenuated the cytotoxic effects and reduced the expression of proinflammatory cytokines, including interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-18 (IL-18), in P. aeruginosa-infected H292 cells. These effects were associated with the inhibition of NF-κB signaling and inflammasome activation. Taken together, our findings suggest that PYC may serve as a promising therapeutic candidate against P. aeruginosa infections by targeting T3SS-mediated virulence and modulating host inflammatory responses.

The photosensitizer DH-I-180-3 regulates intracellular bacterial growth by increasing the secretion of proinflammatory cytokines via the NF-κB- and MAPK-mediated signaling pathways and promoting phagosome maturation in Salmonella-infected mouse macrophages: Hyo-Jung Kim, Eui-Kwon Jeong, Hyo-Ji Lee, Yu-Jin Jung; J. Microbiol. 2025;63(6):e2502003. Published online June 4, 2025; DOI: https://doi.org/10.71150/jm.2502003

1,312 View
55 Download
1 Web of Science
1 Crossref

Abstract PDF

Photodynamic therapy (PDT) is a known strategy for treating cancer; in PDT, photosensitizers are activated by light stimulation and then induce reactive oxygen species (ROS) production to damage cancer tissues. Recently evidence has shown that PDT can also be used as a novel treatment strategy to control pathogenic bacteria. In previous studies, the photosensitizer DH-I-180-3 was reported to effectively regulate multidrug-resistant Mycobacterium tuberculosis growth. Here, we confirmed the effects of DH-I-180-3 on the antibacterial activity and inflammatory response of macrophages to Salmonella. Photoactivated DH-I-180-3 regulated intracellular bacterial growth in Salmonella-infected macrophages. Moreover, DH-I-180-3 increased intracellular ROS levels in Salmonella-infected macrophages. The phosphorylation of the intracellular signaling proteins IκBα and JNK1/2 was increased in DH-I-180-3-treated Salmonella-infected macrophages. Additionally, we observed that DH-I-180-3 significantly increased the mRNA expression and protein secretion of the proinflammatory cytokine TNF-α and promoted phagosome maturation by upregulating EEA1, LAMP1, and Cathepsin D in Salmonella-infected macrophages. Overall, these results demonstrate that photoactivated DH-I-180-3 enhances the bactericidal response to intracellular bacterial infection by promoting inflammatory signaling pathways and phagosome maturation. Therefore, DH-I-180-3 has the potential to be developed into PDT for treating bacterial-infection.

Citations

Citations to this article as recorded by

Transcriptome Analysis Reveals Circadian Rhythmic Regulation of Lipid Metabolism and Immune Function in Chicken Livers
Jiahua Li, Jie Dong, Minjie Huang, Yuting Jin, Xiaodong Tan, Deqian Wang
Animals.2025; 15(22): 3241. CrossRef

Alizarin, which reduces ExoS, attenuates inflammation by P. aeruginosa in H292 cells: Seung-Ho Kim, Hye In Ahn, Jae-Hoon Oh, Da Yun Seo, Jung-Hee Kim, Ok-kyoung Kwon, Ji-Won Park, Kyung-Seop Ahn; J. Microbiol. 2025;63(5):e2411012. Published online May 27, 2025; DOI: https://doi.org/10.71150/jm.2411012

1,461 View
31 Download
1 Web of Science
1 Crossref

Abstract PDF

Pseudomonas aeruginosa (P. aeruginosa) is resistant to several drugs as well as antibiotics and is thus classified as multidrug resistant and extensively drug resistant. These bacteria have a secretion system called the "type 3 secretion system (T3SS)", which facilitates infection by delivering an effector protein. ExoenzymeS (ExoS) is known to induce cell death and activate caspase-1. In particular, patients infected with P. aeruginosa develop diseases associated with high mortality, such as pneumonia, because no drug targets an ExoS or T3SS. We selected natural compounds to treat T3SS-mediated pneumonia and chose alizarin, a red dye. We confirmed the effects of alizarin on T3SS by bacterial PCR and ELISA. It was confirmed that alizarin regulates ExoS by inhibiting exsA but also popD and pscF. Furthermore, in infected H292 cells, it not only attenuates inflammation by inhibiting lipopolysaccharide (LPS)-induced phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 but also interferes with the level of ExoS delivered into the host and modulates caspase-1. We confirmed this result and determined that it led to decreases in proinflammatory cytokines such as Interleukin-1beta (IL-1β), Interleukin-6 (IL-6), and Interleukin-18 (IL-18). Therefore, we suggest that alizarin is a suitable drug for treating pneumonia caused by P. aeruginosa because it helps to attenuate inflammation by regulating T3SS and NF-κB signaling.

Citations

Citations to this article as recorded by

Beyond pathogenicity: applications of the type III secretion system (T3SS) of Pseudomonas aeruginosa
Tianqi Su, Lin Zhang, Jie Shen, Danyu Qian, Yulei Guo, Zhenpeng Li
Frontiers in Microbiology.2025;[Epub] CrossRef

Journal Articles

H-NS is a Transcriptional Repressor of the CRISPR-Cas System in Acinetobacter baumannii ATCC 19606: Kyeongmin Kim, Md Maidul Islam, Seunghyeok Bang, Jeongah Kim, Chung-Young Lee, Je Chul Lee, Minsang Shin; J. Microbiol. 2024;62(11):999-1012. Published online November 11, 2024; DOI: https://doi.org/10.1007/s12275-024-00182-5

448 View
7 Download
1 Web of Science
2 Crossref

Abstract PDF

Acinetobacter baumannii is a multidrug-resistant opportunistic pathogen primarily associated with hospital-acquired infections. The bacterium can gain multidrug resistance through several mechanisms, including horizontal gene transfer. A CRISPR-Cas system including several Cas genes could restrict the horizontal gene transfer. However, the molecular mechanism of CRISPR- Cas transcriptional regulation remains unclear. We identified a type I-F CRISPR-Cas system in A. baumannii ATCC 19606^T standard strain based on sequence analysis. We focused on the transcriptional regulation of Cas3, a key protein of the CRISPR-Cas system. We performed a DNA affinity chromatography-pulldown assay to identify transcriptional regulators of the Cas3 promoter. We identified several putative transcriptional factors, such as H-NS, integration host factor, and HU, that can bind to the promoter region of Cas3. We characterized AbH-NS using size exclusion chromatography and cross-linking experiments and demonstrated that the Cas3 promoter can be regulated by AbH-NS in a concentration-dependent manner via an in vitro transcription assay. CRISPR-Cas expression levels in wild-type and hns mutant strains in the early stationary phase were examined by qPCR and β-galactosidase assay. We found that H-NS can act as a repressor of Cas3. Our transformation efficiency results indicated that the hns mutation decreased the transformation efficiency, while the Cas3 mutation increased it. We report the existence and characterization of the CRISPR-Cas system in A. baumannii 19606^T and demonstrate that AbH-NS is a transcriptional repressor of CRISPR-Cas-related genes in A. baumannii.

Citations

Citations to this article as recorded by

The H-NS homologues MvaT and MvaU repress CRISPR-Cas in Pseudomonas aeruginosa
Kira Céline Koonce, Jesper Juel Mauritzen, Ida Friberg Hitz, Emil Funk Vangsgaard, Elizabeth H. M. Putz, Anne Sofie Wajn, Frederik Hagelund Leth, Nina Molin Høyland-Kroghsbo
Philosophical Transactions of the Royal Society B: Biological Sciences.2025;[Epub] CrossRef
BaeR and H-NS control CRISPR-Cas-mediated immunity and virulence in Acinetobacter baumannii
Ting Yu, Jun Xie, Xinyue Huang, Jiayuan Huang, Guangyu Bao, Wenjie Yuan, Chengfeng Gao, Cuicui Liu, Jian Hu, Weixuan Yang, Guocai Li, Ryan McClure
mSystems.2025;[Epub] CrossRef

Whole-Genome Sequencing Reveals the Population Structure and Genetic Diversity of Salmonella Typhimurium ST34 and ST19 Lineages: Zhen-Xu Zhuo, Yu-Lian Feng, Xi-Wei Zhang, Hao Liu, Fang-Yin Zeng, Xiao-Yan Li; J. Microbiol. 2024;62(10):859-870. Published online November 4, 2024; DOI: https://doi.org/10.1007/s12275-024-00170-9

337 View
10 Download
1 Crossref

Abstract PDF

Salmonella Typhimurium is an invasive gastrointestinal pathogen for both humans and animals. To investigate the genetic framework and diversity of S. Typhimurium, a total of 194 S. Typhimurium isolates were collected from patients in a tertiary hospital between 2020 and 2021. Antimicrobial susceptibility testing was used to confirm the resistance phenotype. Whole-genome sequencing and bioinformatics analysis were performed to determine the sequence type, phylogenetic relationships, resistance gene profiles, Salmonella pathogenicity island (SPI) and the diversity of the core and pan genome. The result showed that 57.22% of S. Typhimurium isolates were multidrug resistant and resistance of total isolates to the first-line drug ciprofloxacin was identified in 60.82%. The population structure of S. Typhimurium was categorized into three lineages: ST19 (20.10%, 39/194), ST34-1 (47.42%, 92/194) and ST34-2 (40.65%, 63/194), with the population size exhibiting increasing trends. All lineages harbored variety of fimbrial operons, prophages, SPIs and effectors that contributed to the virulence and long-term infections of S. Typhimurium. Importantly, ST34-1 lineage might potentially be more invasive due to the possession of SPI1-effector gene sopE which was essential for the proliferation, internalization and intracellular presence of S. Typhimurium in hosts. Multiple antimicrobial resistance genes were characteristically distributed across three lineages, especially carbapenem genes only detected in ST34-1&2 lineages. The distinct functional categories of pan genome among three lineages were observed in metabolism, signaling and gene information processing. This study provides a theoretical foundation for the evolved adaptation and genetic diversity of S. Typhimurium ST19 and ST34, among which ST34 lineages with multidrug resistance and potential hypervirulence need to pay more attention to epidemiological surveillance.

Citations

Citations to this article as recorded by

Genomic Evidence for the Rise of Salmonella Typhimurium ST34 with Increased Plasmid-Mediated Resistance in the Thailand Pork Chain
Hongmei Liu, Ning Wang, Sunpetch Angkititrakul, Wengui Li, Zhongyang Luo, Mingpeng Hou, Yi Wu, Yubo Shi, Yuelin Wang, Fengyun Li, Yaowen Liu, Xin Wu, Fanan Suksawat
Pathogens.2025; 14(12): 1190. CrossRef

Unexpected Requirement of Small Amino Acids at Position 183 for DNA Binding in the Escherichia coli cAMP Receptor Protein: Marcus Carranza, Amanda Rea, Daisy Pacheco, Christian Montiel, Jin Park, Hwan Youn; J. Microbiol. 2024;62(10):871-882. Published online September 6, 2024; DOI: https://doi.org/10.1007/s12275-024-00169-2

440 View
9 Download
2 Web of Science
1 Crossref

Abstract PDF

The Escherichia coli cAMP receptor protein (CRP) relies on the F-helix, the recognition helix of the helix-turn-helix motif, for DNA binding. The importance of the CRP F-helix in DNA binding is well-established, yet there is little information on the roles of its non-base-contacting residues. Here, we show that a CRP F-helix position occupied by a non-base-contacting residue Val183 bears an unexpected importance in DNA binding. Codon randomization and successive in vivo screening selected six amino acids (alanine, cysteine, glycine, serine, threonine, and valine) at CRP position 183 to be compatible with DNA binding. These amino acids are quite different in their amino acid properties (polar, non-polar, hydrophobicity), but one commonality is that they are all relatively small. Larger amino acid substitutions such as histidine, methionine, and tyrosine were made site-directedly and showed to have no detectable DNA binding, further supporting the requirement of small amino acids at CRP position 183. Bioinformatics analysis revealed that small amino acids (92.15% valine and 7.75% alanine) exclusively occupy the position analogous to CRP Val183 in 1,007 core CRP homologs, consistent with our mutant data. However, in extended CRP homologs comprising 3700 proteins, larger amino acids could also occupy the position analogous to CRP Val183 albeit with low occurrence. Another bioinformatics analysis suggested that large amino acids could be tolerated by compensatory small-sized amino acids at their neighboring positions. A full understanding of the unexpected requirement of small amino acids at CRP position 183 for DNA binding entails the verification of the hypothesized compensatory change(s) in CRP.

Citations

Citations to this article as recorded by

SPD_0410 negatively regulates capsule polysaccharide synthesis and virulence in Streptococcus pneumoniae D39
Ye Tao, Li Lei, Shuhui Wang, Xuemei Zhang, Yibing Yin, Yuqiang Zheng
Frontiers in Microbiology.2025;[Epub] CrossRef

Enterococcus Phage vB_EfaS_HEf13 as an Anti-Biofilm Agent Against Enterococcus faecalis: Dongwook Lee, Jintaek Im, A Reum Kim, Woohyung Jun, Cheol-Heui Yun, Seung Hyun Han; J. Microbiol. 2024;62(8):683-693. Published online June 27, 2024; DOI: https://doi.org/10.1007/s12275-024-00150-z

480 View
11 Download
2 Web of Science
2 Crossref

Abstract PDF

Enterococcus faecalis is a Gram-positive bacterium that is frequently found in the periapical lesion of patients with apical periodontitis. Its biofilm formation in root canal is closely related to the development of refractory apical periodontitis by providing increased resistance to endodontic treatments. Phage therapy has recently been considered as an efficient therapeutic strategy in controlling various periodontal pathogens. We previously demonstrated the bactericidal capacities of Enterococcus phage vB_EfaS_HEf13 (phage HEf13) against clinically-isolated E. faecalis strains. Here, we investigated whether phage HEf13 affects biofilm formation and pre-formed biofilm of clinically-isolated E. faecalis, and its combinatory effect with endodontic treatments, including chlorhexidine (CHX) and penicillin. The phage HEf13 inhibited biofilm formation and disrupted pre-formed biofilms of E. faecalis in a dose- and time-dependent manner. Interestingly, phage HEf13 destroyed E. faecalis biofilm exopolysaccharide (EPS), which is known to be a major component of bacterial biofilm. Furthermore, combined treatment of phage HEf13 with CHX or penicillin more potently inhibited biofilm formation and disrupted pre-formed biofilm than either treatment alone. Confocal laser scanning microscopic examination demonstrated that these additive effects of the combination treatments on disruption of pre-formed biofilm are mediated by relatively enhanced reduction in thickness distribution and biomass of biofilm. Collectively, our results suggest that the effect of phage HEf13 on E. faecalis biofilm is mediated by its EPS-degrading property, and its combination with endodontic treatments more potently suppresses E. faecalis biofilm, implying that phage HEf13 has potential to be used as a combination therapy against E. faecalis infections.

Citations

Citations to this article as recorded by

Size-dependent ecotoxicological impacts of tire wear particles on zebrafish physiology and gut microbiota: Implications for aquatic ecosystem health
Yun Zhang, Qianqian Song, Qingxuan Meng, Tianyu Zhao, Xiaolong Wang, Xinrui Meng, Jing Cong
Journal of Hazardous Materials.2025; 487: 137215. CrossRef
Phage therapy as a revitalized weapon for treating clinical diseases
Yingjie Wang, Yamei Yu
Microbiome Research Reports.2025;[Epub] CrossRef

Repeated Exposure of Vancomycin to Vancomycin-Susceptible Staphylococcus aureus (VSSA) Parent Emerged VISA and VRSA Strains with Enhanced Virulence Potentials: An Nguyen, J Jean Sophy Roy, Ji-Hoon Kim, Kyung-Hee Yun, Wonsik Lee, Kyeong Kyu Kim, Truc Kim, Akhilesh Kumar Chaurasia; J. Microbiol. 2024;62(7):535-553. Published online May 30, 2024; DOI: https://doi.org/10.1007/s12275-024-00139-8

425 View
8 Download
2 Web of Science
2 Crossref

Abstract PDF

The emergence of resistance against the last-resort antibiotic vancomycin in staphylococcal infections is a serious concern for human health. Although various drug-resistant pathogens of diverse genetic backgrounds show higher virulence potential, the underlying mechanism behind this is not yet clear due to variability in their genetic dispositions. In this study, we investigated the correlation between resistance and virulence in adaptively evolved isogenic strains. The vancomycin-susceptible Staphylococcus aureus USA300 was exposed to various concentrations of vancomycin repeatedly as a mimic of the clinical regimen to obtain mutation(s)-accrued-clonally-selected (MACS) strains. The phenotypic analyses followed by expression of the representative genes responsible for virulence and resistance of MACS strains were investigated. MACS strains obtained under 2 and 8 µg/ml vancomycin, named Van2 and Van8, respectively; showed enhanced vancomycin minimal inhibitory concentrations (MIC) to 4 and 16 µg/ml, respectively. The cell adhesion and invasion of MACS strains increased in proportion to their MICs. The correlation between resistance and virulence potential was partially explained by the differential expression of genes known to be involved in both virulence and resistance in MACS strains compared to parent S. aureus USA300. Repeated treatment of vancomycin against vancomycin-susceptible S. aureus (VSSA) leads to the emergence of vancomycin-resistant strains with variable levels of enhanced virulence potentials.

Citations

Citations to this article as recorded by

Targeting the G-quadruplex as a novel strategy for developing antibiotics against hypervirulent drug-resistant Staphylococcus aureus
Maria Sultan, Maria Razzaq, Joohyun Lee, Shreyasi Das, Shrute Kannappan, Vinod Kumar Subramani, Wanki Yoo, Truc Kim, Hye-Ra Lee, Akhilesh K. Chaurasia, Kyeong Kyu Kim
Journal of Biomedical Science.2025;[Epub] CrossRef
Staphylococcus parequorum sp. nov. and Staphylococcus halotolerans sp. nov., isolated from traditional Korean soybean foods
Ju Hye Baek, Dong Min Han, Dae Gyu Choi, Chae Yeong Moon, Jae Kyeong Lee, Chul-Hong Kim, Jung-Woong Kim, Che Ok Jeon
Journal of Microbiology.2025; 63(8): e2503003. CrossRef

Genetically Engineered CLDN18.2 CAR-T Cells Expressing Synthetic PD1/CD28 Fusion Receptors Produced Using a Lentiviral Vector: Heon Ju Lee, Seo Jin Hwang, Eun Hee Jeong, Mi Hee Chang; J. Microbiol. 2024;62(7):555-568. Published online May 3, 2024; DOI: https://doi.org/10.1007/s12275-024-00133-0

510 View
11 Download
3 Web of Science
4 Crossref

Abstract PDF

This study aimed to develop synthetic Claudin18.2 (CLDN18.2) chimeric antigen receptor (CAR)-T (CAR-T) cells as a treatment for advanced gastric cancer using lentiviral vector genetic engineering technology that targets the CLDN18.2 antigen and simultaneously overcomes the immunosuppressive environment caused by programmed cell death protein 1 (PD-1). Synthetic CAR T cells are a promising approach in cancer immunotherapy but face many challenges in solid tumors. One of the major problems is immunosuppression caused by PD-1. CLDN18.2, a gastric-specific membrane protein, is considered a potential therapeutic target for gastric and other cancers. In our study, CLDN18.2 CAR was a second-generation CAR with inducible T-cell costimulatory (CD278), and CLDN18.2-PD1/CD28 CAR was a third-generation CAR, wherein the synthetic PD1/CD28 chimeric-switch receptor (CSR) was added to the second-generation CAR. In vitro, we detected the secretion levels of different cytokines and the killing ability of CAR-T cells. We found that the secretion of cytokines such as interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) secreted by three types of CAR-T cells was increased, and the killing ability against CLDN18.2-positive GC cells was enhanced. In vivo, we established a xenograft GC model and observed the antitumor effects and off-target toxicity of CAR-T cells. These results support that synthetic anti-CLDN18.2 CAR-T cells have antitumor effect and anti-CLDN18.2-PD1/CD28 CAR could provide a promising design strategy to improve the efficacy of CAR-T cells in advanced gastric cancer.

Citations

Citations to this article as recorded by

Enhancing the antitumor activity of CD19/BCMA CAR-T cells in vitro with a PD1IL7R chimeric switch receptor
Kai Yan, Zhongdang Xiao
Cellular Immunology.2025; 415-416: 105001. CrossRef
Research progress on mechanisms of tumor immune microenvironment and gastrointestinal resistance to immunotherapy: mini review
Zheng Zhang, Yangping Wu
Frontiers in Immunology.2025;[Epub] CrossRef
On-target off-tumor toxicity of claudin18.2-directed CAR-T cells in preclinical models
Filippo Birocchi, Antonio J. Almazan, Aiyana Parker, Amanda A. Bouffard, Sadie Goncalves, Christopher Kelly, Jessica Frank, Mark B. Leick, Nicholas J. Haradhvala, Shaw Kagawa, Gad Getz, Giulia Escobar, Diego Salas-Benito, Adele Mucci, Trisha R. Berger, Ma
Nature Communications.2025;[Epub] CrossRef
Innovative CAR-T approaches targeting Claudin 18.2 to counteract drug resistance in gastric cancer
Giovanni Calice, Carlo Calabrese, Tiziana Notarangelo
Biomedicine & Pharmacotherapy.2025; 193: 118863. CrossRef

Transcriptomic Insights into Archaeal Nitrification in the Amundsen Sea Polynya, Antarctica: Joo-Han Gwak , Samuel Imisi Awala , So-Jeong Kim , Sang-Hoon Lee , Eun-Jin Yang , Jisoo Park , Jinyoung Jung , Sung-Keun Rhee; J. Microbiol. 2023;61(11):967-980. Published online December 7, 2023; DOI: https://doi.org/10.1007/s12275-023-00090-0

359 View
0 Download
2 Web of Science
2 Crossref

Abstract PDF

Antarctic polynyas have the highest Southern Ocean summer primary productivity, and due to anthropogenic climate change, these areas have formed faster recently. Ammonia-oxidizing archaea (AOA) are among the most ubiquitous and abundant microorganisms in the ocean and play a primary role in the global nitrogen cycle. We utilized metagenomics and metatranscriptomics to gain insights into the physiology and metabolism of AOA in polar oceans, which are associated with ecosystem functioning. A polar-specific ecotype of AOA, from the “Candidatus Nitrosomarinus”-like group, was observed to be dominant in the Amundsen Sea Polynya (ASP), West Antarctica, during a succession of summer phytoplankton blooms. AOA had the highest transcriptional activity among prokaryotes during the bloom decline phase (DC). Metatranscriptomic analysis of key genes involved in ammonia oxidation, carbon fixation, transport, and cell division indicated that this polar AOA ecotype was actively involved in nitrification in the bloom DC in the ASP. This study revealed the physiological and metabolic traits of this key polar-type AOA in response to phytoplankton blooms in the ASP and provided insights into AOA functions in polar oceans.

Citations

Citations to this article as recorded by

Bulk metagenomics and machine learning unravels nitrogen metabolism patterns in extreme-temperature marine environments
Zheng Guo, Yong-Guang Li, Xiao-Lin Liu, Zhao-Jie Teng, Qi-Long Qin, Qian-Qian Cha, Zhi-Bin Wang, Shou-Qing Ni
Bioresource Technology.2026; 441: 133551. CrossRef
Alleviated photoinhibition on nitrification in the Indian Sector of the Southern Ocean
Lingfang Fan, Min Chen, Zifei Yang, Minfang Zheng, Yusheng Qiu
Acta Oceanologica Sinica.2024; 43(7): 52. CrossRef

Development of a Novel Korean H9‑Specific rRT‑PCR Assay and Its Application for Avian Influenza Virus Surveillance in Korea: Mingeun Sagong , Yong-Myung Kang , Na Yeong Kim , Eun Bi Noh , Gyeong-Beom Heo , Se-Hee An , Youn-Jeong Lee , Young Ki Choi , Kwang-Nyeong Lee; J. Microbiol. 2023;61(10):929-936. Published online November 27, 2023; DOI: https://doi.org/10.1007/s12275-023-00088-8

401 View
0 Download
3 Web of Science
3 Crossref

Abstract PDF

Since the 2000s, the Y439 lineage of H9N2 avian influenza virus (AIV) has been the predominant strain circulating in poultry in Korea; however, in 2020, the Y280 lineage emerged and spread rapidly nationwide, causing large economic losses. To prevent further spread and circulation of such viruses, rapid detection and diagnosis through active surveillance programs are crucial. Here, we developed a novel H9 rRT-PCR assay that can detect a broad range of H9Nx viruses in situations in which multiple lineages of H9 AIVs are co-circulating. We then evaluated its efficacy using a large number of clinical samples. The assay, named the Uni Kor-H9 assay, showed high sensitivity for Y280 lineage viruses, as well as for the Y439 lineage originating in Korean poultry and wild birds. In addition, the assay showed no cross-reactivity with other subtypes of AIV or other avian pathogens. Furthermore, the Uni Kor-H9 assay was more sensitive, and had higher detection rates, than reference H9 rRT-PCR methods when tested against a panel of domestically isolated H9 AIVs. In conclusion, the novel Uni Kor-H9 assay enables more rapid and efficient diagnosis than the “traditional” method of virus isolation followed by subtyping RT-PCR. Application of the new H9 rRT-PCR assay to AI active surveillance programs will help to control and manage Korean H9 AIVs more efficiently.

Citations

Citations to this article as recorded by

Development of a multiplex and universal RT-PCR assay for hemagglutinin and neuraminidase subtyping of avian influenza virus
Se-Hee An, Gyeong-Beom Heo, Yong-Myung Kang, Youn-Jeong Lee, Kwang-Nyeong Lee
Journal of Veterinary Science.2025;[Epub] CrossRef
A Systematic Review of New, Enhanced Surveillance Systems and Methodologies for Zoonotic Influenza Viruses in Animals and Human–Animal Interface
Rebecca Badra, Wenqing Zhang, John S. L. Tam, Richard Webby, Sylvie Van Der Werf, Sergejs Nikisins, Ann Cullinane, Saad Gharaibeh, Richard Njouom, Malik Peiris, Ghazi Kayali, Jean‐Michel Heraud
Influenza and Other Respiratory Viruses.2025;[Epub] CrossRef
Development and evaluation of a multiplex real-time RT-PCR assay for simultaneous detection of H5, H7, and H9 subtype avian influenza viruses
Se-Hee An, Na-Yeong Kim, Gyeong-Beom Heo, Yong-Myung Kang, Youn-Jeong Lee, Kwang-Nyeong Lee
Journal of Virological Methods.2024; 327: 114942. CrossRef

NEDD4 Regulated Pyroptosis Occurred from Co‑infection between Influenza A Virus and Streptococcus pneumoniae: Jiangzhou You , Linlin Zhou , Xudong San , Hailing Li , Mingyuan Li , Baoning Wang; J. Microbiol. 2023;61(8):777-789. Published online October 4, 2023; DOI: https://doi.org/10.1007/s12275-023-00076-y

360 View
0 Download
9 Web of Science
8 Crossref

Abstract PDF

Co-infection of respiratory tract viruses and bacteria often result in excess mortality, especially pneumonia caused by influenza viruses and Streptococcus pneumoniae. However, the synergistic mechanisms remain poorly understood. Therefore, it is necessary to develop a clearer understanding of the molecular basis of the interaction between influenza virus and Streptococcus pneumonia. Here, we developed the BALB/c mouse model and the A549 cell model to investigate inflammation and pyroptotic cell death during co-infection. Co-infection significantly activated the NLRP3 inflammasome and induced pyroptotic cell death, correlated with excess mortality. The E3 ubiquitin ligase NEDD4 interacted with both NLRP3 and GSDMD, the executor of pyroptosis. NEDD4 negatively regulated NLRP3 while positively regulating GSDMD, thereby modulating inflammation and pyroptotic cell death. Our findings suggest that NEDD4 may play a crucial role in regulating the GSDMD-mediated pyroptosis signaling pathway. Targeting NEDD4 represents a promising approach to mitigate excess mortality during influenza pandemics by suppressing synergistic inflammation during co-infection of influenza A virus and Streptococcus pneumoniae.

Citations

Citations to this article as recorded by

GSDMD and GSDME exhibit distinct roles in enteric coronavirus PDCoV-induced pyroptosis and inflammatory responses
Chenyu Li, Yuting Shi, Chunying Xie, Kaiqi Duan, Tong Ding, Xiangfei Xu, Liurong Fang, Yanrong Zhou, Shaobo Xiao, Tom Gallagher
Journal of Virology.2025;[Epub] CrossRef
Updated insights into the molecular networks for NLRP3 inflammasome activation
Seungwha Paik, Jin Kyung Kim, Hyo Jung Shin, Eun-Jin Park, In Soo Kim, Eun-Kyeong Jo
Cellular & Molecular Immunology.2025; 22(6): 563. CrossRef
Universal and highly sensitive detection of influenza A virus and streptococcus pneumoniae using WGA-modified magnetic SERS nanotags-based lateral flow assay
Xiaofei Jia, Zhenzhen Liu, Juan Zhou, Chunran Cao, Yunwei Hao, Jin Chen, Han Han, Jing Liang, Zhibin Zhao, Yi Wang, Zhendong Niu, Rui Xiao
Nanomedicine: Nanotechnology, Biology and Medicine.2025; 69: 102853. CrossRef
Post-influenza bacterial infection: mechanisms of pathogenesis and advances in therapeutic strategies
Biao Lei, Shun Wang, Linzhong Yu, Qinhai Ma
Frontiers in Microbiology.2025;[Epub] CrossRef
Pyroptosis in Respiratory Virus Infections: A Narrative Review of Mechanisms, Pathophysiology, and Potential Therapeutic Interventions
Runqi Lin, Barbara N. Porto
Microorganisms.2025; 13(9): 2109. CrossRef
Yinqin Qingfei granules alleviate Mycoplasma pneumoniae pneumonia via inhibiting NLRP3 inflammasome-mediated macrophage pyroptosis
Zhe Song, Chengen Han, Guangzhi Luo, Guangyuan Jia, Xiao Wang, Baoqing Zhang
Frontiers in Pharmacology.2024;[Epub] CrossRef
Overexpression of DTX1 inhibits D-GalN/TNF-α-induced pyroptosis and inflammation in hepatocytes by regulating NLRP3 ubiquitination
Mingshui Liu, Jing Gu, Li Chen, Wei Sun, Xiaoping Huang, Jianhe Gan
Toxicology Research.2024;[Epub] CrossRef
NLRP3 Inflammasomes: Dual Function in Infectious Diseases
Yanbo Li, Rui Qiang, Zhengmin Cao, Qingjuan Wu, Jiuchong Wang, Wenliang Lyu
The Journal of Immunology.2024; 213(4): 407. CrossRef

Comparison of Conjunctival Sac Microbiome between Low and High Myopic Eyes: Kang Xiao , Zhengyu Chen , Qin Long; J. Microbiol. 2023;61(5):571-578. Published online April 21, 2023; DOI: https://doi.org/10.1007/s12275-023-00045-5

400 View
0 Download
4 Web of Science
2 Crossref

Abstract PDF

Microbial communities played a vital role in maintaining homeostasis of ocular surface. However, no studies explored the myopia-associated conjunctiva microbiota changes until now. In this study, conjunctival sac swab specimens were collected from 12 eyes of low myopia (LM), and 14 eyes of high myopia (HM) patients. The V3–V4 region of the 16S rRNA gene was amplified and then sequenced. Statistical analysis was performed to investigate differences in the taxonomy and diversity between two groups. Compared to LM, higher Ocular Surface Disease Index (OSDI) scores were observed in HM group. The Shannon index of the HM was lower than that of the LM group (P = 0.017). Principle coordinate analysis and Partial Least Squares Discrimination Analysis showed distinct microbiome composition between two groups. At the phylum level, there were higher relative abundances of Proteobacteria (68.27% vs 38.51%) and lower abundances of Actinobacteria (3.71% vs 9.19%) in HM, compared to LM group (P = 0.031, 0.010, respectively). At the genus level, the abundances of Acinetobacter in HM (18.16%) were significantly higher than the LM (6.52%) group (P = 0.011). Actinobacteria levels were negatively correlated with the myopic spherical equivalent and OSDI scores. Moreover, positive correlations were found between Proteobacteria levels and OSDI scores, Acinetobacter levels were positively correlated with myopic spherical equivalent and OSDI scores. In conclusion, HM Patients have bacterial microbiota imbalance in the conjunctival sac, compared with LM patients. Proteobacteria, Actinobacteria, Acinetobacter may play roles in the HM associated ocular surface irritation.

Citations

Citations to this article as recorded by

Harnessing Lactiplantibacillus plantarum EP21 and its membrane vesicles to inhibit myopia development
Chi-Fong Lin, Yu-An Hsu, Yung-Lan Chou, Ying-Chi Chen, En-Shyh Lin, Peng-Tai Tien, Jamie jiin-Yi Chen, Ming-Yen Wu, Chia-Hung Lin, Hui-Ju Lin, Lei Wan
Gut Microbes.2025;[Epub] CrossRef
Gut Microbiota Profiles in Myopes and Nonmyopes
Wan E. W. Omar, Gurdeep Singh, Andrew J. McBain, Fiona Cruickshank, Hema Radhakrishnan
Investigative Ophthalmology & Visual Science.2024; 65(5): 2. CrossRef

Vaginal Microbiome Dysbiosis is Associated with the Different Cervical Disease Status: Yingying Ma , Yanpeng Li , Yanmei Liu , Le Cao , Xiao Han , Shujun Gao , Chiyu Zhang; J. Microbiol. 2023;61(4):423-432. Published online April 3, 2023; DOI: https://doi.org/10.1007/s12275-023-00039-3

454 View
0 Download
14 Web of Science
15 Crossref

Abstract PDF

Vaginal microbiome composition was demonstrated to be associated with cervical disease. The colonization characteristics of vaginal microbes and their association with the different cervical disease status, especially cervical cancer (CC), are rarely investigated. In this cross-sectional study, we characterized the vaginal microbiome of women with different status of cervical diseases, including 22 NV + (normal tissue with HPV infection), low-grade squamous intraepithelial lesion (LSIL, n = 45), high-grade squamous intraepithelial lesion (HSIL, n = 36) and CC (n = 27) using bacterial 16S DNA sequencing. Thirty HPV-negative women with normal tissue were used as the control group. We found that higher diversity of microbiome with gradual depletion of Lactobacillus, especially L. crispatus, was associated with the severity of cervical disease. High-risk HPV16 infection was associated with higher microbiome diversity and depletion of Lactobacillus in high-grade cervical diseases (i.e. HSIL and CC). The CC group was characterized by higher levels of Fannyhessea vaginae, Prevotella, Bacteroides, Finegoldia, Vibrio, Veillonella, Peptostreptococcus, and Dialister. Co-occurrence network analyses showed that negative correlations were exclusively observed between Lactobacillus and other bacteria, and almost all non-Lactobacillus bacteria were positively correlated with each other. In particular, the most diverse and complex co-occurrence network of vaginal bacteria, as well as a complete loss of L. crispatus, was observed in women with CC. Logistic regression model identified HPV16 and Lactobacillus as significant risk and protective factors for CC, respectively. These results suggest that specific Lactobacillus species (e.g. L. crispatus and L. iners) can be used as important markers to target prevention measures prioritizing HPV16-infected women and other hrHPV-infected women for test, vaccination and treat initiatives.

Citations

Citations to this article as recorded by

Vaginal Microbiota and Local Immunity in HPV-Induced High-Grade Cervical Dysplasia: A Narrative Review
Helena C. J. Schellekens, Lotte M. S. Schmidt, Servaas A. Morré, Edith M. G. van Esch, Peggy J. de Vos van Steenwijk
International Journal of Molecular Sciences.2025; 26(9): 3954. CrossRef
The Vaginal Microbiota, Human Papillomavirus, and Cervical Dysplasia—A Review
Justė Kazlauskaitė, Guoda Žukienė, Vilius Rudaitis, Daiva Bartkevičienė
Medicina.2025; 61(5): 847. CrossRef
Nanoparticles and the Vaginal Microbiota: Diagnostic and Therapeutic Innovations in Human Papilloma Virus-associated Cervical Cancer – A Systematic Review
Saranya Velmurugan, Karthikeyan Ganesan, Archana Rajasundaram, C. Thangam, Rozario Cyril, Gowtham Kumar Subbaraj
Nigerian Postgraduate Medical Journal.2025; 32(1): 1. CrossRef
Exploring the Interplay Between Cervicovaginal Microbiome, HPV Infection, and Cervical Intraepithelial Neoplasia in Taiwanese Women
Chung‐Yao Yang, Ting‐Chang Chang, Yi‐Tzu Lee, Ting‐Ying Shih, Chang‐Wei Li, Chao‐Min Cheng
Journal of Medical Virology.2025;[Epub] CrossRef
A nomogram prediction model for embryo implantation outcomes based on the cervical microbiota of the infertile patients during IVF-FET
Yanan Wu, Lingyun Shi, Zili Jin, Wenjun Chen, Fuxin Wang, Huihua Wu, Hong Li, Ce Zhang, Rui Zhu, Simone Filardo
Microbiology Spectrum.2025;[Epub] CrossRef
The Interplay Between Cervicovaginal Microbiota Diversity, Lactobacillus Profiles and Human Papillomavirus in Cervical Cancer: A Systematic Review
Giosuè Giordano Incognito, Carlo Ronsini, Vittorio Palmara, Paola Romeo, Giuseppe Vizzielli, Stefano Restaino, Marco La Verde, Orazio De Tommasi, Marco Palumbo, Stefano Cianci
Healthcare.2025; 13(6): 599. CrossRef
Associations of the gut, cervical, and vaginal microbiota with cervical cancer: a systematic review and meta-analysis
Qin Wen, Shubin Wang, Yalan Min, Xinyi Liu, Jian Fang, Jinyi Lang, Meihua Chen
BMC Women's Health.2025;[Epub] CrossRef
Quantification of Lactobacillus spp. of interest for the study of the vaginal microbiota
Vivian Heimbecker, Bárbara Pontarollo Dal Santos, Ana Paula Thomaz, Keite da Silva Nogueira, Camila Marconi
Journal of Microbiological Methods.2025; 236: 107158. CrossRef
Associations of Atopobium, Garderella, Megasphaera, Prevotella, Sneathia, and Streptococcus with human papillomavirus infection, cervical intraepithelial neoplasia, and cancer: a systematic review and meta-analysis
Yan Peng, Qin Tang, Shiming Wu, Chengzhi Zhao
BMC Infectious Diseases.2025;[Epub] CrossRef
Role of Vaginal and Gut Microbiota in Human Papillomavirus (HPV) Progression and Cervical Cancer: A Systematic Review of Microbial Diversity and Probiotic Interventions
Hrishikesh D Pai, Rashmi Baid, Nandita P Palshetkar, Rishma Pai, Arnav Pai, Rohan Palshetkar
Cureus.2025;[Epub] CrossRef
Translating the vaginal microbial landscape: a connecting link between bacterial vaginosis and preeclampsia
Devanshi Gajjar, Sriram Seshadri
Exploration of Immunology.2025;[Epub] CrossRef
Vaginal Microbiome and Pregnancy Complications: A Review
Angeliki Gerede, Konstantinos Nikolettos, Eleftherios Vavoulidis, Chrysoula Margioula-Siarkou, Stamatios Petousis, Maria Giourga, Panagiotis Fotinopoulos, Maria Salagianni, Sofoklis Stavros, Konstantinos Dinas, Nikolaos Nikolettos, Ekaterini Domali
Journal of Clinical Medicine.2024; 13(13): 3875. CrossRef
Advancements in the Vaginal Microenvironment and Regression of High-Risk Human Papillomavirus
Na He, Cunjian Yi, Qingsong Zeng, Wumei Jing, Wenrong He
Indian Journal of Microbiology.2024;[Epub] CrossRef
Research Progress on Related Factors of Cervical High-Grade Squamous Intraepithelial Lesions
红颖王
Advances in Clinical Medicine.2023; 13(12): 20536. CrossRef
Role of the vaginal microbiome in miscarriage: exploring the relationship
Marwa Saadaoui, Parul Singh, Osman Ortashi, Souhaila Al Khodor
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef

Rhizosphere Microbial Community and Metabolites of Susceptible and Resistant Tobacco Cultivars to Bacterial Wilt: Wan Zhao , Yanyan Li , Chunlei Yang , Yong Yang , Yun Hu; J. Microbiol. 2023;61(4):389-402. Published online March 7, 2023; DOI: https://doi.org/10.1007/s12275-023-00012-0

372 View
0 Download
7 Web of Science
6 Crossref

Abstract PDF

Soil-borne diseases are closely related to rhizosphere microecosystem. While, plant species and genotypes are important factors affected rhizosphere microecosystem. In this study, the rhizosphere soil microbial community and metabolites of susceptible and resistant tobacco cultivars were investigated. The results showed that there were significant differences in the rhizosphere microbial community and metabolites between susceptible cultivar Yunyan87 and resistant cultivar Fandi3. Furthermore, the rhizosphere soil of Fandi3 showed a higher microbial diversity than that of Yunyan87. The abundance of R. solanacearum was much higher in the rhizosphere soil of Yunyan87 than in the rhizosphere soil of Fandi3, resulting in a higher disease incidence and index. While the abundance of beneficial bacteria in the rhizosphere soil of Fandi3 were higher than that of Yunyan87. Additionally, there were significant differences in metabolites between Yunyan87 and Fandi3 cultivars, and 4-hydroxybenzaldehyde, 3-hydroxy-4-methoxybenzoic acid, vamillic aldehyde, benzoic acid, 4-hydroxybenzyl alcohol, p-hydroxybenzoic acid and phthalic acid were notably high in Yunyan87. Redundancy analysis (RDA) indicated that the rhizosphere microbial community of Fandi3 and Yunyan87 were highly correlated with various environmental factors and metabolites. Overall, susceptible and resistant tobacco cultivars had different impact on rhizosphere microbial community and metabolites. The results expand our understanding of the roles of tobacco cultivars in plant-micro-ecosystem interactions, and provide a basis for the control of tobacco bacterial wilt.

Citations

Citations to this article as recorded by

Diversity and composition of soil microbial communities in the rhizospheres of late blight-resistant tomatoes after Phytophthora infestans inoculation
Xinyan Zhou, Liyuan Liao, Ken Chen, Yan Yin, Lulu Qiu, Xinni Li, Qingshan Li, Shangdong Yang
Frontiers in Plant Science.2025;[Epub] CrossRef
MAPK Cascades in Plant Microbiota Structure and Functioning
Thijs Van Gerrewey, Hoo Sun Chung
Journal of Microbiology.2024; 62(3): 231. CrossRef
Response of Soil Microorganisms and Phenolic to Pseudostelariae heterophylla Cultivation in Different Soil Types
Yingying Liu, Dan Wu, Yongjun Kan, Li Zhao, Chang Jiang, Wensheng Pang, Juan Hu, Meilan Zhou
Eurasian Soil Science.2024; 57(3): 446. CrossRef
Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysis
Chengjian Wei, Jinchang Liang, Rui Wang, Luping Chi, Wenjing Wang, Jun Tan, Heli Shi, Xueru Song, Zhenzhen Cui, Qiang Xie, Dejie Cheng, Xiaoqiang Wang
Frontiers in Plant Science.2024;[Epub] CrossRef
The Composition and Function of the Rhizosphere Bacterial Community of Paeonia lactiflora Varies with the Cultivar
Liping Yang, Xin Wan, Runyang Zhou, Yingdan Yuan
Biology.2023; 12(11): 1363. CrossRef
Analysis of the response mechanisms of Pinellia ternata to terahertz wave stresses using transcriptome and metabolic data
Dongdong Wang, Surendra Sarsaiya, Xu Qian, Leilei Jin, Fuxing Shu, Chuanyou Zhang, Jishuang Chen
Frontiers in Plant Science.2023;[Epub] CrossRef

The Revision of Lichen Flora Around Maxwell Bay, King George Island, Maritime Antarctic: Jae Eun So , Josef P. Halda , Soon Gyu Hong , Jae&# , Ji Hee Kim; J. Microbiol. 2023;61(2):159-173. Published online February 27, 2023; DOI: https://doi.org/10.1007/s12275-023-00015-x

479 View
0 Download
5 Web of Science
4 Crossref

Abstract PDF

Since the floristic study of lichens at the Barton and Weaver Peninsulas of King George Island in 2006, there have been intense investigations of the lichen flora of the two peninsulas as well as that of Fildes Peninsula and Ardley Island in Maxwell Bay, King George Island, South Shetland Islands, maritime Antarctic. In this study, a total of 104 species belonging to 53 genera, are identified from investigations of lichens that were collected in austral summer seasons from 2008 to 2016. Phenotypic and molecular analyses were incorporated for taxonomic identification. In particular, 31 species are found to be endemic to the Antarctic and 22 species are newly recorded to the Maxwell Bay region. Lepra dactylina, Stereocaulon caespitosum, and Wahlenbergiella striatula are newly recorded in the Antarctic, and the previously reported taxon Cladonia furcata is excluded from the formerly recorded list due to misidentification. We also provide ecological and geographical information about lichen associations and habitat preferences.

Citations

Citations to this article as recorded by

Lichens and Health—Trends and Perspectives for the Study of Biodiversity in the Antarctic Ecosystem
Tatiana Prado, Wim Maurits Sylvain Degrave, Gabriela Frois Duarte
Journal of Fungi.2025; 11(3): 198. CrossRef
Antioxidant and Antidiabetic Potential of the Antarctic Lichen Gondwania regalis Ethanolic Extract: Metabolomic Profile and In Vitro and In Silico Evaluation
Alfredo Torres-Benítez, José Erick Ortega-Valencia, Nicolás Jara-Pinuer, Jaqueline Stephanie Ley-Martínez, Salvador Herrera Velarde, Iris Pereira, Marta Sánchez, María Pilar Gómez-Serranillos, Ferdinando Carlo Sasso, Mario Simirgiotis, Alfredo Caturano
Antioxidants.2025; 14(3): 298. CrossRef
Morphology and molecular characterization of a new Chloroidium (Trebouxiophyceae, Chlorophyta) species isolated from lichen in Antarctica
Hyunsik Chae, Yung Mi Lee, Hyodong Lee, Jae Eun So, Jeongha So, Han‐Gu Choi, Sanghee Kim, Ji Hee Kim
Phycological Research.2025; 73(2): 125. CrossRef
Lichens of Larsemann Hills and adjacent oases in the area of Prydz Bay (Princess Elizabeth Land and MacRobertson Land, Antarctica)
Mikhail Andreev (Mихаил АНДРЕЕВ)
Polar Science.2023; 38: 101009. CrossRef

Genome Sequencing Highlights the Plant Cell Wall Degrading Capacity of Edible Mushroom Stropharia rugosoannulata: Mengpei Guo , Xiaolong Ma , Yan Zhou , Yinbing Bian , Gaolei Liu , Yingli Cai , Tianji Huang , Hongxia Dong , Dingjun Cai , Xueji Wan , Zhihong Wang , Yang Xiao , Heng Kang; J. Microbiol. 2023;61(1):83-93. Published online February 1, 2023; DOI: https://doi.org/10.1007/s12275-022-00003-7

424 View
0 Download
11 Web of Science
9 Crossref

Abstract PDF

The basidiomycetous edible mushroom Stropharia rugosoannulata has excellent nutrition, medicine, bioremediation, and biocontrol properties. S. rugosoannulata has been widely and easily cultivated using agricultural by-products showing strong lignocellulose degradation capacity. However, the unavailable high-quality genome information has hindered the research on gene function and molecular breeding of S. rugosoannulata. This study provided a high-quality genome assembly and annotation from S. rugosoannulata monokaryotic strain QGU27 based on combined Illumina-Nanopore data. The genome size was about 47.97 Mb and consisted of 20 scaffolds, with an N50 of 3.73 Mb and a GC content of 47.9%. The repetitive sequences accounted for 17.41% of the genome, mostly long terminal repeats (LTRs). A total of 15,726 coding gene sequences were putatively identified with the BUSCO score of 98.7%. There are 142 genes encoding plant cell wall degrading enzymes (PCWDEs) in the genome, and 52, 39, 30, 11, 8, and 2 genes related to lignin, cellulose, hemicellulose, pectin, chitin, and cutin degradation, respectively. Comparative genomic analysis revealed that S. rugosoannulata is superior in utilizing aldehyde-containing lignins and is possible to utilize algae during the cultivation.

Citations

Citations to this article as recorded by

Analysis of Gene Regulatory Network and Transcription Factors in Different Tissues of the Stropharia rugosoannulata Fruiting Body
Jia Lu, Jing Yan, Na Lu, Jiling Song, Jiayao Lin, Xiaohua Zhou, Xuebing Ying, Zhen Li, Zufa Zhou, Fangjie Yao
Journal of Fungi.2025; 11(2): 123. CrossRef
Livestock–Crop–Mushroom (LCM) Circular System: An Eco-Friendly Approach for Enhancing Plant Performance and Mitigating Microbiological Risks
Dong Liu, Yousif Abdelrahman Yousif Abdellah, Tingting Dou, Katharina Maria Keiblinger, Ziyan Zhou, Parag Bhople, Jishao Jiang, Xiaofei Shi, Fengming Zhang, Fuqiang Yu, Baoshan Xing
Environmental Science & Technology.2025; 59(17): 8541. CrossRef
Crop–Mushroom Rotation: A Comprehensive Review of Its Multifaceted Impacts on Soil Quality, Agricultural Sustainability, and Ecosystem Health
Tingting Dou, Kaixuan Zhang, Xiaofei Shi, Wei Liu, Fuqiang Yu, Dong Liu
Agronomy.2025; 15(3): 563. CrossRef
High-Resolution Core Gene-Associated Multiple Nucleotide Polymorphism (cgMNP) Markers for Strain Identification in the Wine Cap Mushroom Stropharia rugosoannulata
Fei Liu, Bin Cao, Hongmei Dai, Guojie Li, Shoumian Li, Wei Gao, Ruilin Zhao
Microorganisms.2025; 13(7): 1685. CrossRef
Isolation and Structural Characterization of Melanins from Red and Yellow Varieties of Stropharia rugosoannulata
Zhen-Fei Xie, Wei-Wei Zhang, Shun-Yin Zhao, Xiao-Han Zhang, Shu-Ning You, Chun-Mei Liu, Guo-Qing Zhang
International Journal of Molecular Sciences.2025; 26(14): 6985. CrossRef
The rise of Stropharia rugosoannulata industry in China: current state and prospects
Lei Huang, Can Si, Chun-mei He, Xun-cheng Liu, Jun Duan
Applied Microbiology and Biotechnology.2025;[Epub] CrossRef
Evaluation of Genetic Diversity and Agronomic Traits of Germplasm Resources of Stropharia rugosoannulata
Miao Gu, Qiang Chen, Yan Zhang, Yongchang Zhao, Li Wang, Xiangli Wu, Mengran Zhao, Wei Gao
Horticulturae.2024; 10(3): 213. CrossRef
Molecular Profiling of Rice Straw Degradability Discrepancy in Stropharia rugosoannulata Core Germplasm
Wenbing Gong, Yuyu Zeng, Xinru Li, Zhidong Zhao, Nan Shen, Yan Zhou, Yinbing Bian, Yang Xiao
Journal of Agricultural and Food Chemistry.2024; 72(45): 25379. CrossRef
RETRACTED ARTICLE: Genome assembly of M. spongiola and comparative genomics of the genus Morchella provide initial insights into taxonomy and adaptive evolution
Qing Meng, Zhanling Xie, Hongyan Xu, Jing Guo, Qingqing Peng, Yanyan Li, Jiabao Yang, Deyu Dong, Taizhen Gao, Fan Zhang
BMC Genomics.2024;[Epub] CrossRef

Potential Use of Mycobacterium paragordonae for Antimycobacterial Drug Screening Systems: Ga-Yeong Cha , Hyejun Seo , Jaehun Oh , Byoung-Jun Kim , Bum-Joon Kim; J. Microbiol. 2023;61(1):121-129. Published online January 31, 2023; DOI: https://doi.org/10.1007/s12275-022-00009-1

414 View
0 Download
1 Web of Science
2 Crossref

Abstract PDF

Our recent genome-based study indicated that Mycobacterium paragordonae (Mpg) has evolved to become more adapted to an intracellular lifestyle within free-living environmental amoeba and its enhanced intracellular survival within Acanthamoeba castellanii was also proved. Here, we sought to investigate potential use of Mpg for antimycobacterial drug screening systems. Our data showed that Mpg is more susceptible to various antibiotics compared to the close species M. marinum (Mmar) and M. gordonae, further supporting its intracellular lifestyle in environments, which would explain its protection from environmental insults. In addition, we developed two bacterial whole-cell-based drug screening systems using a recombinant Mpg stain harboring a luciferase reporter vector (rMpg-LuxG13): one for direct application to rMpg-LuxG13 and the other for drug screening via the interaction of rMpg-LuxG13 with A. castellanii. Direct application to rMpg-LuxG13 showed lower inhibitory concentration 50 ( IC50) values of rifampin, isoniazid, clarithromycin, and ciprofloxacin against Mpg compared to Mmar. Application of drug screening system via the interaction of rMpg-LuxG13 with A. castellanii also exhibited lower IC50 values for rifampin against Mpg compared to Mmar. In conclusion, our data indicate that Mpg is more susceptible to various antibiotics than other strains. In addition, our data also demonstrate the feasibility of two whole cellbased drug screening systems using rMpg-LuxG13 strain for the discovery of novel anti-mycobacterial drugs.

Citations

Citations to this article as recorded by

Mycobacterium paragordonae: Insights into its Research Progress and Potential Applications
Hyejun Seo, Ju-Young Lee, Bum-Joon Kim
Journal of Bacteriology and Virology.2024; 54(4): 273. CrossRef
Protection against tuberculosis achieved by dissolving microneedle patches loaded with live Mycobacterium paragordonae in a BCG prime-boost strategy
Mi-Hyun Lee, Hyejun Seo, Moon-Su Lee, Byoung Jun Kim, Hye Lin Kim, Du Hyung Lee, Jaehun Oh, Ju Yeop Shin, Ju Young Jin, Do Hyeon Jeong, Bum-Joon Kim
Frontiers in Immunology.2023;[Epub] CrossRef

Coumarin-based combined computational study to design novel drugs against Candida albicans: Akhilesh Kumar Maurya , Nidhi Mishra; J. Microbiol. 2022;60(12):1201-1207. Published online November 10, 2022; DOI: https://doi.org/10.1007/s12275-022-2279-5

521 View
0 Download
5 Web of Science
4 Crossref

Abstract PDF

Candida species cause the most prevalent fungal illness, candidiasis. Candida albicans is known to cause bloodstream infections. This species is a commensal bacterium, but it can cause hospital–acquired diseases, particularly in COVID-19 patients with impaired immune systems. Candida infections have increased in patients with acute respiratory distress syndrome. Coumarins are both naturally occurring and synthetically produced. In this study, the biological activity of 40 coumarin derivatives was used to create a three-dimensional quantitative structure activity relationship (3D-QSAR) model. The training and test minimum inhibitory concentration values of C. albicans active compounds were split, and a regression model based on statistical data was established. This model served as a foundation for the creation of coumarin derivative QSARs. This is a unique way to create new therapeutic compounds for various ailments. We constructed novel structural coumarin derivatives using the derived QSAR model, and the models were confirmed using molecular docking and molecular dynamics simulation.

Citations

Citations to this article as recorded by

Synthesis, molecular docking and anti-biofilm activity of novel benzo[4,5]imidazo[2,1-a]quinazoline, 4H-chromene, and acridine derivatives as potent anti-candida agents
Farid M. Sroor, Ahmed Younis, Mohamed Abdelraof, Ismail A. Abdelhamid
Journal of Molecular Structure.2025; 1331: 141520. CrossRef
Coumarin derivatives ameliorate the intestinal inflammation and pathogenic gut microbiome changes in the model of infectious colitis through antibacterial activity
Hui-su Jung, Yei Ju Park, Bon-Hee Gu, Goeun Han, Woonhak Ji, Su mi Hwang, Myunghoo Kim
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Therapeutic Effects of Coumarins with Different Substitution Patterns
Virginia Flores-Morales, Ana P. Villasana-Ruíz, Idalia Garza-Veloz, Samantha González-Delgado, Margarita L. Martinez-Fierro
Molecules.2023; 28(5): 2413. CrossRef
Cyclometalated iridium(III) complexes combined with fluconazole: antifungal activity against resistant C. albicans
Jun-Jian Lu, Zhi-Chang Xu, Hou Zhu, Lin-Yuan Zhu, Xiu-Rong Ma, Rui-Rui Wang, Rong-Tao Li, Rui-Rong Ye
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef

Genomic and physiological analysis of C50 carotenoid-producing novel Halorubrum ruber sp. nov.: Chi Young Hwang , Eui-Sang Cho , Won Jong Rhee , Eunjung Kim , Myung-Ji Seo; J. Microbiol. 2022;60(10):1007-1020. Published online August 26, 2022; DOI: https://doi.org/10.1007/s12275-022-2173-1

366 View
1 Download
8 Web of Science
9 Crossref

Abstract PDF

A novel haloarchaeal species designated as MBLA0099T was isolated from seawater near Yeongheung Island. Cells were Gram-negative, non-motile, red-pigmented, and rod-shaped. They grew at 10–45°C, within pH 5.5–9.0, and between 7.5% and 30% NaCl concentrations. Cells were able to grow without Mg2+ and were lysed in distilled water. The size of the whole-genome and G + C content of DNA was 3.02 Mb and 68.9 mol%, respectively. Phylogenetic analysis shows that the strain MBLA0099T belongs to the genus Halorubrum. The average nucleotide and amino acid identity, and in silico DNA-DNA hybridization values were below the species delineation threshold. Pan-genomic analysis revealed that 3.2% of all genes present in strain MBLA0099T were unique to the strain. The red carotenoid produced by strain MBLA0099T was subjected to spectrometric and chromatographic analyses and confirmed to be bacterioruberin as C50 carotenoid. Mevalonic acid, terpenoid backbone, and carotenoid biosynthesis pathway were annotated for strain MBLA0099T. The C50 carotenoid production by strain MBLA0099T was also enhanced under various stress conditions including relatively netural pH, high oxidative and salinity conditions. Additionally, the strain MBLA0099T-derived bacterioruberin showed the antioxidant activity with EC50 value of 12.29 μg/ml, based on the evaluation of DPPH free radical scavenging activity. The present study would be the first report on the identification of C50 carotenoid from the strain MBLA0099T representing a novel species of the genus Halorubrum, for which the name Halorubrum ruber sp. nov. is proposed. The typestrain used was MBLA0099T (= KCTC 4296T = JCM 34701T).

Citations

Citations to this article as recorded by

Genomic insights into Halorubrum ezzemoulense strain TC23: Genetic basis for halophilic traits and biotechnological potential
Fevziye Işıl Kesbiç
Science Progress.2025;[Epub] CrossRef
Genome-based classification of Halonovum salinarum gen. nov., sp. nov., and Halonovum rutilum sp. nov., two novel halophilic archaea isolated from a solar saltern
Chi Young Hwang, Eui-Sang Cho, Ki-Eun Lee, Eun-Young Lee, Myung-Ji Seo
BMC Microbiology.2025;[Epub] CrossRef
Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential
Chi Young Hwang, Eui-Sang Cho, Sungjun Kim, Kyobum Kim, Myung-Ji Seo
Microbial Cell Factories.2024;[Epub] CrossRef
Microbial Bacterioruberin: A Comprehensive Review
Mouliraj Palanisamy, Sathishkumar Ramalingam
Indian Journal of Microbiology.2024; 64(4): 1477. CrossRef
Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. Within the OM60/NOR5 Clade, Isolated from Seawater, and Emended Description of the Genus Congregibacter
Hyeonsu Tak, Miri S. Park, Hyerim Cho, Yeonjung Lim, Jang-Cheon Cho
Journal of Microbiology.2024; 62(9): 739. CrossRef
Genomic insights on carotenoid synthesis by extremely halophilic archaea Haloarcula rubripromontorii BS2, Haloferax lucentense BBK2 and Halogeometricum borinquense E3 isolated from the solar salterns of India
Devika. N. Nagar, Kabilan Mani, Judith M. Braganca
Scientific Reports.2024;[Epub] CrossRef
Microbial bacterioruberin: The new C50 carotenoid player in food industries
Cassamo U. Mussagy, Angie V. Caicedo-Paz, Fabiane O. Farias, Leonardo M. de Souza Mesquita, Daniele Giuffrida, Laurent Dufossé
Food Microbiology.2024; 124: 104623. CrossRef
Evaluation of Various Escherichia coli Strains for Enhanced Lycopene Production
Jun Ren, Junhao Shen, Thi Duc Thai, Min-gyun Kim, Seung Ho Lee, Wonseop Lim, Dokyun Na
Journal of Microbiology and Biotechnology.2023; 33(7): 973. CrossRef
Bioactive molecules from haloarchaea: Scope and prospects for industrial and therapeutic applications
Jamseel Moopantakath, Madangchanok Imchen, V. T. Anju, Siddhardha Busi, Madhu Dyavaiah, Rosa María Martínez-Espinosa, Ranjith Kumavath
Frontiers in Microbiology.2023;[Epub] CrossRef

Review

The crosstalk between bacteria and host autophagy: host defense or bacteria offense: Lin Zheng , Fang Wei , Guolin Li; J. Microbiol. 2022;60(5):451-460. Published online April 29, 2022; DOI: https://doi.org/10.1007/s12275-022-2009-z

352 View
0 Download
6 Web of Science
7 Crossref

Abstract PDF

Xenophagy is a specific selective autophagy for the elimination of intracellular bacteria. Current evidence suggests that the processes for host autophagy system to recognize and eliminate invading bacteria are complex, and vary according to different pathogens. Although both ubiquitin-dependent and ubiquitin-independent autophagy exist in host to defense invading bacteria, successful pathogens have evolved diverse strategies to escape from or paralyze host autophagy system. In this review, we discuss the mechanisms of host autophagy system to recognize and eliminate intracellular pathogens and the mechanisms of different pathogens to escape from or paralyze host autophagy system, with a particular focus on the most extensively studied bacteria.

Citations

Citations to this article as recorded by

Complement C3 deposition restricts the proliferation of internalized Staphylococcus aureus by promoting autophagy
Yining Deng, Yunke Zhang, Tong Wu, Kang Niu, Xiaoyu Jiao, Wenge Ma, Chen Peng, Wenxue Wu
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Innate immune response of host cells infected with Salmonella
Hongfei Fan, Juane Lu, Hao Wu, Haihua Ruan, Wenjun Song, Y.-T. Yu, P.P. Piccaluga, S. Xie
BIO Web of Conferences.2024; 111: 01022. CrossRef
Exploring the Connections: Autophagy, Gut Microbiota, and Inflammatory Bowel Disease Pathogenesis
Arunkumar Subramanian, Afrarahamed J, Tamilanban T, Vinoth Kumarasamy, M Yasmin Begum, Mahendran Sekar, Vetriselvan Subramaniyan, Ling Shing Wong, Adel Al Fatease
Journal of Inflammation Research.2024; Volume 17: 10453. CrossRef
Programmed cell death and Salmonella pathogenesis: an interactive overview
Yu Zhang, Maodou Xu, Yujiao Guo, Li Chen, Wanwipa Vongsangnak, Qi Xu, Lizhi Lu
Frontiers in Microbiology.2024;[Epub] CrossRef
Bacterial lipoprotein plays an important role in the macrophage autophagy and apoptosis induced by Salmonella typhimurium and Staphylococcus aureus
Shanshan Jiang, Jinyao He, Lijie Zhang, Qiaojiajie Zhao, Shuqi Zhao
Open Life Sciences.2023;[Epub] CrossRef
Xenophagy as a Strategy for Mycobacterium leprae Elimination during Type 1 or Type 2 Leprosy Reactions: A Systematic Review
Débora Dantas Nucci Cerqueira, Ana Letícia Silva Pereira, Ana Elisa Coelho da Costa, Tarcísio Joaquim de Souza, Matheus Santos de Sousa Fernandes, Fabrício Oliveira Souto, Patrícia d’Emery Alves Santos
Pathogens.2023; 12(12): 1455. CrossRef
Brucella BtpB Manipulates Apoptosis and Autophagic Flux in RAW264.7 Cells
Junmei Li, Lin Qi, Ziyang Diao, Mengyu Zhang, Bin Li, Yunyi Zhai, Mingyue Hao, Dong Zhou, Wei Liu, Yaping Jin, Aihua Wang
International Journal of Molecular Sciences.2022; 23(22): 14439. CrossRef

Journal Articles

Gamma-glutamyltransferase of Helicobacter pylori alters the proliferation, migration, and pluripotency of mesenchymal stem cells by affecting metabolism and methylation status: Zeyu Wang , Weijun Wang , Huiying Shi , Lingjun Meng , Xin Jiang , Suya Pang , Mengke Fan , Rong Lin; J. Microbiol. 2022;60(6):627-639. Published online April 18, 2022; DOI: https://doi.org/10.1007/s12275-022-1575-4

373 View
0 Download
14 Web of Science
8 Crossref

Abstract PDF

Virulence factor gamma-glutamyltransferase (GGT) of H. pylori consumes glutamine (Gln) in the stomach to decrease the tricarboxylic acid metabolite alpha-ketoglutarate (α-kg) and alter the downstream regulation of α-kg as well as cellular biological characteristics. Our previous research indicated that under H. pylori infection, mesenchymal stem cells (MSCs) migrated to the stomach and participated in gastric cancer (GC) development either by differentiating into epithelial cells or promoting angiogenesis. However, how MSCs themselves participate in H. pylori-indicated GC remains unclear. Therefore, a GGT knockout H. pylori strain (Hp- KS-1) was constructed, and downstream histone H3K9 and H3K27 methylation and the PI3K/AKT signaling pathway of α-kg were detected using Western blotting. The biological characteristics of MSCs were also examined. An additive α-kg supplement was also added to H. pylori-treated MSCs to investigate alterations in these aspects. Compared to the control and Hp-KS-1 groups, H. pylori-treated MSCs reduced Gln and α-kg, increased H3K9me3 and H3K27me3, activated the PI3K-AKT signaling pathway, and promoted the proliferation, migration, self-renewal, and pluripotency of MSCs. The addition of α-kg rescued the H. pylori-induced alterations. Injection of MSCs to nude mice resulted in the largest tumors in the H. pylori group and significantly reduced tumor sizes in the Hp-KS-1 and α-kg groups. In summary, GGT of H. pylori affected MSCs by interfering with the metabolite α-kg to increase trimethylation of histone H3K9 and H3K27, activating the PI3K/AKT signaling pathway, and promoting proliferation, migration, self-renewal, and pluripotency in tumorigenesis, elucidating the mechanisms of MSCs in GC development.

Citations

Citations to this article as recorded by

Helicobacter pylori infection promotes the formation of the β‐catenin/HIF‐1α complex, enabling adaptive responses in gastric cancer cells
Héctor A. Tapia, Camila García‐Navarrete, Patricio Silva, Joaquín Lizana, Carla Fonfach, Ignacio Pezoa‐Soto, Tania Flores, Nadia Hernández, Daniel Peña‐Oyarzún, Jorge Toledo, Safka Hernández‐Gutiérrez, Daniela Herrera, Manuel Varas‐Godoy, Denisse Bravo, V
The FEBS Journal.2025;[Epub] CrossRef
Glucose Metabolism Recoding Regulates the Correa Cascade Response: An Important Strategy for Gastric Cancer Prevention and Treatment in Traditional Chinese Medicine
玉霞龚
Traditional Chinese Medicine.2025; 14(07): 3119. CrossRef
Advances and challenges in Helicobacter pylori subunit vaccine development: antigen candidates and immunization strategies
Zhili Liu, He Li, Xiaotian Huang, Qiong Liu
Journal of Applied Microbiology.2025;[Epub] CrossRef
Gamma-glutamyl transferase secreted by Helicobacter pylori promotes the development of gastric cancer by affecting the energy metabolism and histone methylation status of gastric epithelial cells
Xin Jiang, Weijun Wang, Zeyu Wang, Zhe Wang, Huiying Shi, Lingjun Meng, Suya Pang, Mengke Fan, Rong Lin
Cell Communication and Signaling.2024;[Epub] CrossRef
Design of a Helicobacter pylori multi-epitope vaccine based on immunoinformatics
Man Cui, Xiaohui Ji, Fengtao Guan, Guimin Su, Lin Du
Frontiers in Immunology.2024;[Epub] CrossRef
Gastric cancer and mesenchymal stem cell-derived exosomes: from pro-tumorigenic effects to anti-cancer vehicles
Maryam Dolatshahi, Ahmad Reza Bahrami, Qaiser Iftikhar Sheikh, Mohsen Ghanbari, Maryam M. Matin
Archives of Pharmacal Research.2024; 47(1): 1. CrossRef
Mesenchymal Stem Cell-Derived Exosomes Modulate Angiogenesis in Gastric Cancer
Fawzy Akad, Veronica Mocanu, Sorin Nicolae Peiu, Viorel Scripcariu, Bogdan Filip, Daniel Timofte, Florin Zugun-Eloae, Magdalena Cuciureanu, Monica Hancianu, Teodor Oboroceanu, Laura Condur, Radu Florin Popa
Biomedicines.2023; 11(4): 1031. CrossRef
Helicobacter pylori and Its Role in Gastric Cancer
Victor E. Reyes
Microorganisms.2023; 11(5): 1312. CrossRef

Lysobacter ciconiae sp. nov., and Lysobacter avium sp. nov., isolated from the faeces of an Oriental stork: So-Yeon Lee , Pil Soo Kim , Hojun Sung , Dong-Wook Hyun , Jin-Woo Bae; J. Microbiol. 2022;60(5):469-477. Published online March 31, 2022; DOI: https://doi.org/10.1007/s12275-022-1647-5

392 View
0 Download
7 Web of Science
6 Crossref

Abstract PDF

Two Gram-stain-negative, mesophilic, strictly aerobic, nonspore forming, and yellow-pigmented strains with rod-shaped cells, designated H21R20T and H23M41T, were isolated from the faeces of an Oriental stork (Ciconia boyciana). Based on 16S rRNA gene sequences, both strains showed the highest similarity (98.3−98.4%) to the type strain of Lysobacter concretionis. Phylogenetic analysis based on the 16S rRNA genes and 92 bacterial core genes showed that strains H21R20T and H23M41T were robustly clustered with L. concretionis Ko07T. Whole genome sequencing revealed that the genomes of both strains were approximately 2.9 Mb in size. The DNA G + C contents of the H21R20T and H23M41T strains were 67.3 and 66.6%, respectively. The two strains showed 80.1−81.7% average nucleotide identity with L. concretionis Ko07T. Strain H21R20T grew optimally at 30°C and pH 8.0 and in the presence of 0.5–3% (wt/vol) NaCl, while strain H23M41T grew optimally at 30°C and pH 7.0–8.0 and in the presence of 0–3% (wt/vol) NaCl. Both strains possessed iso-C15:0, iso-C16:0 and summed feature 9 (iso-C17:1 ω9c and/or C16:0 10-methyl) as the major cellular fatty acids, ubiquinone Q-8 as a predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as the major polar lipids. A multifaceted investigation demonstrated that strains H21R20T and H23M41T represent novel species of the genus Lysobacter, for which we propose the names Lysobacter ciconiae sp. nov. and Lysobacter avium sp. nov. for strains H21R20T (= KCTC 82316T = JCM 34832T) and H23M41T (= KCTC 62676T = JCM 33223T), respectively.

Citations

Citations to this article as recorded by

Isolation and Characterization of an Antioxidant Aryl Polyene Pigment from Antarctic Bacterium Lysobacter sp. A03
Maria Elisa Pailliè-Jiménez, Paolo Stincone, Jamile Queiroz Pereira, Patricio Román Santagapita, Eliseu Rodrigues, Adriano Brandelli
Molecular Biotechnology.2024;[Epub] CrossRef
Luteimonas flava sp. nov. and Aquilutibacter rugosus gen. nov., sp. nov., isolated from freshwater environments in China and re-examining the taxonomic status of genera Luteimonas and Lysobacter
Huibin Lu, Li Chen, Yujing Wang, Peng Xing, Qinglong Wu
International Journal of Systematic and Evolutionary Microbiology .2024;[Epub] CrossRef
An update on novel taxa and revised taxonomic status of bacteria isolated from non-domestic animals described in 2022
Claire R. Burbick, Sara D. Lawhon, Erik Munson, Elizabeth Thelen, Amanda Zapp, Anastasia Wilson, Romney M. Humphries
Journal of Clinical Microbiology.2023;[Epub] CrossRef
Lysobacter changpingensis sp. nov., a novel species of the genus Lysobacter isolated from a rhizosphere soil of strawberry in China
Bang-Yan Niu, Dong-Jun Ren, Fang-Bo Zhang, Hong-Tu Zhu, Hai-Lei Wei, Ming-Chao Ma, Miao Gao
Folia Microbiologica.2023; 68(6): 991. CrossRef
Nocardioides palaemonis sp. nov. and Tessaracoccus palaemonis sp. nov., isolated from the gastrointestinal tract of lake prawn
Do-Yeon Kim, In-Chul Jeong, So-Yeon Lee, Yun-Seok Jeong, Jeong Eun Han, Euon Jung Tak, June-Young Lee, Pil Soo Kim, Dong-Wook Hyun, Jin-Woo Bae
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Validation List no. 207. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, George Garrity
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef

Reviews

T cell responses to SARS-CoV-2 in humans and animals: Sameer-ul-Salam Mattoo , Jinjong Myoung; J. Microbiol. 2022;60(3):276-289. Published online February 14, 2022; DOI: https://doi.org/10.1007/s12275-022-1624-z

386 View
0 Download
10 Web of Science
10 Crossref

Abstract PDF

SARS-CoV-2, the causative agent of COVID-19, first emerged in 2019. Antibody responses against SARS-CoV-2 have been given a lot of attention. However, the armamentarium of humoral and T cells may have differing roles in different viral infections. Though the exact role of T cells in COVID-19 remains to be elucidated, prior experience with human coronavirus has revealed an essential role of T cells in the outcomes of viral infections. Moreover, an increasing body of evidence suggests that T cells might be effective against SARS-CoV-2. This review summarizes the role of T cells in mouse CoV, human pathogenic respiratory CoV in general and SARSCoV- 2 in specific.

Citations

Citations to this article as recorded by

Vaccine Strategies Against RNA Viruses: Current Advances and Future Directions
Kuei-Ching Hsiung, Huan-Jung Chiang, Sebastian Reinig, Shin-Ru Shih
Vaccines.2024; 12(12): 1345. CrossRef
Intradermal Fractional ChAdOx1 nCoV-19 Booster Vaccine Induces Memory T Cells: A Follow-Up Study
Ratchanon Sophonmanee, Perawas Preampruchcha, Jomkwan Ongarj, Bunya Seeyankem, Porntip Intapiboon, Smonrapat Surasombatpattana, Supattra Uppanisakorn, Pasuree Sangsupawanich, Sarunyou Chusri, Nawamin Pinpathomrat
Vaccines.2024; 12(2): 109. CrossRef
Safety and efficacy of COVID-19 vaccine immunization during pregnancy in 1024 pregnant women infected with the SARS-CoV-2 Omicron virus in Shanghai, China
Hongmei Deng, Yinpeng Jin, Minmin Sheng, Min Liu, Jie Shen, Wei Qian, Gang Zou, Yixin Liao, Tiefu Liu, Yun Ling, Xiaohong Fan
Frontiers in Immunology.2024;[Epub] CrossRef
Differential Impact of Spike Protein Mutations on SARS-CoV-2 Infectivity and Immune Evasion: Insights from Delta and Kappa Variants
Tae-Hun Kim, Sojung Bae, Jinjong Myoung
Journal of Microbiology and Biotechnology.2024; 34(12): 2506. CrossRef
Animal models for COVID-19 and tuberculosis
Björn Corleis, Max Bastian, Donata Hoffmann, Martin Beer, Anca Dorhoi
Frontiers in Immunology.2023;[Epub] CrossRef
A mathematical model and simulation scenarios for T and B cells immune response to severe acute respiratory syndrome-coronavirus-2
L Cuesta-Herrera, F Córdova-Lepe, L Pastenes, A D Arencibia, Y Baldera-Moreno, H A Torres-Mantilla
Journal of Physics: Conference Series.2023; 2516(1): 012007. CrossRef
Distinctive Combinations of RBD Mutations Contribute to Antibody Evasion in the Case of the SARS-CoV-2 Beta Variant
Tae-Hun Kim, Sojung Bae, Sunggeun Goo, Jinjong Myoung
Journal of Microbiology and Biotechnology.2023; 33(12): 1587. CrossRef
Two years of COVID-19 pandemic: where are we now?
Jinjong Myoung
Journal of Microbiology.2022; 60(3): 235. CrossRef
Escape and Over-Activation of Innate Immune Responses by SARS-CoV-2: Two Faces of a Coin
Sameer-ul-Salam Mattoo, Seong-Jun Kim, Dae-Gyun Ahn, Jinjong Myoung
Viruses.2022; 14(3): 530. CrossRef
Development of a T Cell-Based COVID-19 Vaccine Using a Live Attenuated Influenza Vaccine Viral Vector
Irina Isakova-Sivak, Ekaterina Stepanova, Victoria Matyushenko, Sergei Niskanen, Daria Mezhenskaya, Ekaterina Bazhenova, Elena Krutikova, Tatiana Kotomina, Polina Prokopenko, Bogdan Neterebskii, Aleksandr Doronin, Elena Vinogradova, Kirill Yakovlev, Konst
Vaccines.2022; 10(7): 1142. CrossRef

SARS-CoV-2-mediated evasion strategies for antiviral interferon pathways: Soo-Jin Oh , Ok Sarah Shin; J. Microbiol. 2022;60(3):290-299. Published online February 5, 2022; DOI: https://doi.org/10.1007/s12275-022-1525-1

428 View
0 Download
16 Web of Science
17 Crossref

Abstract PDF

With global expansion of the COVID-19 pandemic and the emergence of new variants, extensive efforts have been made to develop highly effective antiviral drugs and vaccines against SARS-CoV-2. The interactions of coronaviruses with host antiviral interferon pathways ultimately determine successful viral replication and SARS-CoV-2-induced pathogenesis. Innate immune receptors play an essential role in host defense against SARS-CoV-2 via the induction of IFN production and signaling. Here, we summarize the recent advances in innate immune sensing mechanisms of SARS-CoV-2 and various strategies by which SARS-CoV-2 antagonizes antiviral innate immune signaling pathways, with a particular focus on mechanisms utilized by multiple SARS-CoV-2 proteins to evade interferon induction and signaling in host cell. Understanding the underlying immune evasion mechanisms of SARS-CoV-2 is essential for the improvement of vaccines and therapeutic strategies.

Citations

Citations to this article as recorded by

Efficacy and safety of interferon alpha-2b aerosol therapy for patients infected with the SARS-CoV-2 omicron variant: A randomized controlled single-blind study
Yinpeng Jin, Xianming Meng, Zhiping Qian, Jun Zhao, Ying Lv, Yun Ling, Xiaohong Fan
Cellular Immunology.2025; 414: 104992. CrossRef
Overview of the SARS-CoV-2 nucleocapsid protein
Ahmed Eltayeb, Faisal Al-Sarraj, Mona Alharbi, Raed Albiheyri, Ehab Mattar, Isam M. Abu Zeid, Thamer A. Bouback, Atif Bamagoos, Bassam O. Aljohny, Vladimir N. Uversky, Elrashdy M. Redwan
International Journal of Biological Macromolecules.2024; 260: 129523. CrossRef
Does SARS-CoV-2 Induce IgG4 Synthesis to Evade the Immune System?
Alberto Rubio-Casillas, Elrashdy Redwan, Vladimir Uversky
Biomolecules.2023; 13(9): 1338. CrossRef
Appearance of tolerance-induction and non-inflammatory SARS-CoV-2 spike-specific IgG4 antibodies after COVID-19 booster vaccinations
Marjahan Akhtar, Md. Rashedul Islam, Fatema Khaton, Umma Hany Soltana, Syeda Anoushka Jafrin, Sadia Isfat Ara Rahman, Imam Tauheed, Tasnuva Ahmed, Ishtiakul Islam Khan, Afroza Akter, Zahid Hasan Khan, Md. Taufiqul Islam, Farhana Khanam, Prasanta Kumar Bis
Frontiers in Immunology.2023;[Epub] CrossRef
Inhibition of p38 signaling curtails the SARS-CoV-2 induced inflammatory response but retains the IFN-dependent antiviral defense of the lung epithelial barrier
Aileen Faist, Sebastian Schloer, Angeles Mecate-Zambrano, Josua Janowski, André Schreiber, Yvonne Boergeling, Beate C.G. Conrad, Sriram Kumar, Leonie Toebben, Klaus Schughart, Morris Baumgardt, Mirjana Kessler, Katja Hoenzke, Andreas Hocke, Marcel Trautma
Antiviral Research.2023; 209: 105475. CrossRef
Serum neutralization of SARS‐CoV‐2 Omicron BA.2, BA.2.75, BA.2.76, BA.5, BF.7, BQ.1.1 and XBB.1.5 in individuals receiving Evusheld
Qianqian Zhao, Xin Wang, Ze Zhang, Xuefei Liu, Ping Wang, Jin Cao, Qiming Liang, Jieming Qu, Min Zhou
Journal of Medical Virology.2023;[Epub] CrossRef
IgG4 Antibodies Induced by Repeated Vaccination May Generate Immune Tolerance to the SARS-CoV-2 Spike Protein
Vladimir Uversky, Elrashdy Redwan, William Makis, Alberto Rubio-Casillas
Vaccines.2023; 11(5): 991. CrossRef
Warmer ambient air temperatures reduce nasal turbinate and brain infection, but increase lung inflammation in the K18-hACE2 mouse model of COVID-19
Troy Dumenil, Thuy T. Le, Daniel J. Rawle, Kexin Yan, Bing Tang, Wilson Nguyen, Cameron Bishop, Andreas Suhrbier
Science of The Total Environment.2023; 859: 160163. CrossRef
Dexamethasone impairs the expression of antimicrobial mediators in lipopolysaccharide-activated primary macrophages by inhibiting both expression and function of interferon β
John D. O’Neil, Oliwia O. Bolimowska, Sally A. Clayton, Tina Tang, Kalbinder K. Daley, Samuel Lara-Reyna, Jordan Warner, Claire S. Martin, Rahul Y. Mahida, Rowan S. Hardy, J. Simon C. Arthur, Andrew R. Clark
Frontiers in Immunology.2023;[Epub] CrossRef
Selenoprotein S Interacts with the Replication and Transcription Complex of SARS-CoV-2 by Binding nsp7
Farid Ghelichkhani, Fabio A. Gonzalez, Mariia A. Kapitonova, Sharon Rozovsky
Journal of Molecular Biology.2023; 435(8): 168008. CrossRef
Pathogenesis and Mechanisms of SARS-CoV-2 Infection in the Intestine, Liver, and Pancreas
Zaid Khreefa, Mallory T. Barbier, Ali Riza Koksal, Gordon Love, Luis Del Valle
Cells.2023; 12(2): 262. CrossRef
Selenoprotein S: A versatile disordered protein
Farid Ghelichkhani, Fabio A. Gonzalez, Mariia A. Kapitonova, Stephanie Schaefer-Ramadan, Jun Liu, Rujin Cheng, Sharon Rozovsky
Archives of Biochemistry and Biophysics.2022; 731: 109427. CrossRef
Nanomaterials to combat SARS-CoV-2: Strategies to prevent, diagnose and treat COVID-19
Agustín Valenzuela-Fernández, Romina Cabrera-Rodriguez, Laura Ciuffreda, Silvia Perez-Yanes, Judith Estevez-Herrera, Rafaela González-Montelongo, Julia Alcoba-Florez, Rodrigo Trujillo-González, Diego García-Martínez de Artola, Helena Gil-Campesino, Oscar
Frontiers in Bioengineering and Biotechnology.2022;[Epub] CrossRef
Two years of COVID-19 pandemic: where are we now?
Jinjong Myoung
Journal of Microbiology.2022; 60(3): 235. CrossRef
Warmer Ambient Air Temperatures Reduce Nasal Turbinate and Brain Infection, But Increase Lung Inflammation in the K18-hACE2 Mouse Model of COVID-19
Troy Dumenil, Thuy T. Le, Daniel Rawle, Kexin Yan, Bing Tang, Wilson Nguyen, Cameron Bishop, Andreas Suhrbier
SSRN Electronic Journal.2022;[Epub] CrossRef
SARS-CoV-2: A Master of Immune Evasion
Alberto Rubio-Casillas, Elrashdy M. Redwan, Vladimir N. Uversky
Biomedicines.2022; 10(6): 1339. CrossRef
Interferon α-2b spray shortened viral shedding time of SARS-CoV-2 Omicron variant: An open prospective cohort study
Nan Xu, Jinjin Pan, Li Sun, Cuimei Zhou, Siran Huang, Mingwei Chen, Junfei Zhang, Tiantian Zhu, Jiabin Li, Hong Zhang, Yufeng Gao
Frontiers in Immunology.2022;[Epub] CrossRef

Journal Articles

Adaptation of Pseudomonas helmanticensis to fat hydrolysates and SDS: fatty acid response and aggregate formation: Ilya N. Zubkov , Anatoly P. Nepomnyshchiy , Vadim D. Kondratyev , Pavel N. Sorokoumov , Konstantin V. Sivak , Edward S. Ramsay , Sergey M. Shishlyannikov; J. Microbiol. 2021;59(12):1104-1111. Published online October 26, 2021; DOI: https://doi.org/10.1007/s12275-021-1214-5

357 View
0 Download
3 Web of Science
3 Crossref

Abstract PDF

An essential part of designing any biotechnological process is examination of the physiological state of producer cells in different phases of cultivation. The main marker of a bacterial cell’s state is its fatty acid (FA) profile, reflecting membrane lipid composition. Consideration of FA composition enables assessment of bacterial responses to cultivation conditions and helps biotechnologists understand the most significant factors impacting cellular metabolism. In this work, soil SDS-degrading Pseudomonas helmanticensis was studied at the fatty acid profile level, including analysis of rearrangement between planktonic and aggregated forms. The set of substrates included fat hydrolysates, SDS, and their mixtures with glucose. Such media are useful in bioplastic production since they can help incrementally lower overall costs. Conventional gas chromatography-mass spectrometry was used for FA analysis. Acridine orange-stained aggregates were observed by epifluorescence microscopy. The bacterium was shown to change fatty acid composition in the presence of hydrolyzed fats or SDS. These changes seem to be driven by the depletion of metabolizable substrates in the culture medium. Cell aggregation has also been found to be a defense strategy, particularly with anionic surfactant (SDS) exposure. It was shown that simple fluidity indices (such as saturated/ unsaturated FA ratios) do not always sufficiently characterize a cell's physiological state, and morphological examination is essential in cases where complex carbon sources are used.

Citations

Citations to this article as recorded by

Effect of different diet composition on the fat profile of two different black soldier fly larvae populations
M. Tognocchi, L. Abenaim, C. Adamaki-Sotiraki, G.C. Athanassiou, I.C. Rumbos, M. Mele, B. Conti, G. Conte
animal.2024; 18(7): 101205. CrossRef
Earth to Mars: A Protocol for Characterizing Permafrost in the Context of Climate Change as an Analog for Extraplanetary Exploration
Kimberley R. Miner, Joseph Razzell Hollis, Charles E. Miller, Kyle Uckert, Thomas A. Douglas, Emily Cardarelli, Rachel Mackelprang
Astrobiology.2023; 23(9): 1006. CrossRef
Preparation of polyhydroxyalkanoates using Pseudomonas helmanticensis in non-sterile media containing glycerol and sodium dodecyl sulfate
I. N. Zubkov, Yu. S. Bukin, P. N. Sorokoumov, S. M. Shishlyannikov
Proceedings of Universities. Applied Chemistry and Biotechnology.2022; 12(3): 479. CrossRef

The comparison of microbial communities in thyroid tissues from thyroid carcinoma patients: Chen-Jian Liu , Si-Qian Chen , Si-Yao Zhang , Jia-Lun Wang , Xiao-Dan Tang , Kun-Xian Yang , Xiao-Ran Li; J. Microbiol. 2021;59(11):988-1001. Published online October 6, 2021; DOI: https://doi.org/10.1007/s12275-021-1271-9

385 View
0 Download
14 Web of Science
12 Crossref

Abstract PDF

Thyroid carcinoma is a common endocrine organ cancer associated with abnormal hormone secretion, leading to the disorder of metabolism. The intestinal microbiota is vital to maintain digestive and immunologic homeostasis. The relevant information of the microbial community in the gut and thyroid, including composition, structure, and relationship, is unclear in thyroid carcinoma patients. A total of 93 samples from 25 patients were included in this study. The results showed that microbial communities existed in thyroid tissue; gut and thyroid had high abundance of facultative anaerobes from the Proteobacteria phyla. The microbial metabolism from the thyroid and gut may be affected by the thyroid carcinoma cells. The cooccurrence network showed that the margins of different thyroid tissues were unique areas with more competition; the stabilization of microcommunities from tissue and stool may be maintained by several clusters of species that may execute different vital metabolism processes dominantly that are attributed to the microenvironment of cancer.

Citations

Citations to this article as recorded by

Modulation of Intestinal Flora: a Novel Immunotherapeutic Approach for Enhancing Thyroid Cancer Treatment
Weiqiang Huang, Tao Jiang, Jiaxuan He, Jing Ruan, Baihui Wu, Runchao Tao, Peiye Xu, Yongpan Wang, Rongbing Chen, Hanbing Wang, Qinsi Yang, Kun Zhang, Libo Jin, Da Sun, Jinfeng You
Probiotics and Antimicrobial Proteins.2025; 17(3): 1038. CrossRef
The intratumor microbiota and thyroid cancer: a review
Shan Liu, Xiaoliang Xiong, Tingting Hao, Xue Shi, Yinlong Zhao
Frontiers in Endocrinology.2025;[Epub] CrossRef
Recent advances in gut microbiota and thyroid disease: pathogenesis and therapeutics in autoimmune, neoplastic, and nodular conditions
Lihua Fang, Jie Ning
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Causal relationship of genetically predicted gut microbiota with thyroid cancer: a bidirectional two-sample mendelian randomization study
Xiaohe Sun, Shuai Chen, Shuoqi Zhao, Jingwen Wang, Haibo Cheng
Frontiers in Endocrinology.2024;[Epub] CrossRef
Investigating the role of the intratumoral microbiome in thyroid cancer development and progression
Hanieh Ataollahi, Mehdi Hedayati, Noosha Zia-Jahromi, Maryam Daneshpour, Seyed Davar Siadat
Critical Reviews in Oncology/Hematology.2024; 204: 104545. CrossRef
The relationship between thyroid and human-associated microbiota: A systematic review of reviews
Camilla Virili, Ilaria Stramazzo, Maria Flavia Bagaglini, Anna Lucia Carretti, Silvia Capriello, Francesco Romanelli, Pierpaolo Trimboli, Marco Centanni
Reviews in Endocrine and Metabolic Disorders.2024; 25(1): 215. CrossRef
Clinical potential of microbiota in thyroid cancer therapy
Zilan Xie, Jiating Zhou, Xuan Zhang, Zhi Li
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease.2024; 1870(2): 166971. CrossRef
Recent Trends and Potential of Radiotherapy in the Treatment of Anaplastic Thyroid Cancer
Kazumasa Sekihara, Hidetomo Himuro, Soji Toda, Nao Saito, Ryoichi Hirayama, Nobuyasu Suganuma, Tetsuro Sasada, Daisuke Hoshino
Biomedicines.2024; 12(6): 1286. CrossRef
Intratumoral Bacteria Dysbiosis Is Associated with Human Papillary Thyroid Cancer and Correlated with Oncogenic Signaling Pathways
Shuang Yu, Yanqiang Ding, Xuejie Wang, Siu Kin Ng, Siting Cao, Weixin Liu, Zhuming Guo, Yubin Xie, Shubin Hong, Lixia Xu, Xiaoxing Li, Jie Li, Weiming Lv, Sui Peng, Yanbing Li, Joseph J.Y. Sung, Jun Yu, Haipeng Xiao
Engineering.2023; 28: 179. CrossRef
Causal analysis of the gut microbiota in differentiated thyroid carcinoma: a two-sample Mendelian randomization study
Zheng Quan, Xiaoyu Zhang, Shilong Wang, Yong Meng
Frontiers in Genetics.2023;[Epub] CrossRef
Tumor Microbial Communities and Thyroid Cancer Development—The Protective Role of Antioxidant Nutrients: Application Strategies and Future Directions
Francesca Gorini, Alessandro Tonacci
Antioxidants.2023; 12(10): 1898. CrossRef
Interaction of Gut Microbiota with Endocrine Homeostasis and Thyroid Cancer
Qi Liu, Wei Sun, Hao Zhang
Cancers.2022; 14(11): 2656. CrossRef

Characterization of staphylococcal endolysin LysSAP33 possessing untypical domain composition: Jun-Hyeok Yu , Do-Won Park , Jeong-A Lim , Jong-Hyun Park; J. Microbiol. 2021;59(9):840-847. Published online August 12, 2021; DOI: https://doi.org/10.1007/s12275-021-1242-1

335 View
0 Download
8 Web of Science
7 Crossref

Abstract PDF

Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent. Many endolysins on staphylococcal phages have been identified and applied extensively against Staphylococcus spp. Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins. However, relatively little interest has been paid to LysKunlike endolysin and a few of them has been characterized. An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD). A green fluorescence assay using a fusion protein for Lys- SAP33_CBD indicated that the CBD domain (157-251 aa) was bound to the peptidoglycan of S. aureus. The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy. Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations. In addition, it showed a higher activity against biofilms than LysK-like endolysin. This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.

Citations

Citations to this article as recorded by

Phage-Derived Endolysins Against Resistant Staphylococcus spp.: A Review of Features, Antibacterial Activities, and Recent Applications
Mina Golban, Javad Charostad, Hossein Kazemian, Hamid Heidari
Infectious Diseases and Therapy.2025; 14(1): 13. CrossRef
Characterization of a phage endolysin LysLFP01 and its antibacterial activity
Qiannan Wen, Xuecheng Huang, Wenxin Ma, Yingtong Chen, Luyao Wang, Yang Ma, Xia Chen
International Journal of Food Microbiology.2025; 432: 111110. CrossRef
ZAM-CS, a novel chimeric endolysin with enhanced stability and rapid action against methicillin-resistant Staphylococcus aureus
Yasaman Ahmadbeigi, Neda Soleimani, Farzaneh Azizmohseni, Zahra Amini-Bayat
BMC Microbiology.2025;[Epub] CrossRef
Molecular Machinery of the Triad Holin, Endolysin, and Spanin: Key Players Orchestrating Bacteriophage-Induced Cell Lysis and their Therapeutic Applications
Safia Samir
Protein & Peptide Letters.2024; 31(2): 85. CrossRef
A Novel Truncated CHAP Modular Endolysin, CHAPSAP26-161, That Lyses Staphylococcus aureus, Acinetobacter baumannii, and Clostridioides difficile, and Exhibits Therapeutic Effects in a Mouse Model of A. baumannii Infection
Yoon-Jung Choi, Shukho Kim, Ram Hari Dahal, Jungmin Kim
Journal of Microbiology and Biotechnology.2024; 34(8): 1718. CrossRef
Therapeutic potential of bacteriophage endolysins for infections caused by Gram-positive bacteria
He Liu, Zhen Hu, Mengyang Li, Yi Yang, Shuguang Lu, Xiancai Rao
Journal of Biomedical Science.2023;[Epub] CrossRef
Endolysin, a Promising Solution against Antimicrobial Resistance
Mujeeb ur Rahman, Weixiao Wang, Qingqing Sun, Junaid Ali Shah, Chao Li, Yanmei Sun, Yuanrui Li, Bailing Zhang, Wei Chen, Shiwei Wang
Antibiotics.2021; 10(11): 1277. CrossRef

Raman spectroscopy reveals alteration of spore compositions under different nutritional conditions in Lysinibacillus boronitolerans YS11: Youngung Ryu , Minyoung Hong , Soo Bin Kim , Tae Kwon Lee , Woojun Park; J. Microbiol. 2021;59(5):491-499. Published online March 29, 2021; DOI: https://doi.org/10.1007/s12275-021-0679-6

390 View
0 Download
7 Web of Science
7 Crossref

Abstract PDF

Little is known about final spores components when bacteria undergo sporulation under different nutrient conditions. Different degrees of resistance and germination rates were observed in the three types of spores of Lysinibacillus boronitolerans YS11 (SD, Spores formed in Difco sporulation mediumTM; SC and SF, Spores formed in an agricultural byproduct medium with 10 mM CaCl2 and with 10 mM FeSO4, respectively). Stronger UV resistance was recorded for SF with 1.8–2.3-fold greater survival than SC and SD under UV treatment. The three spore types showed similar heat resistances at 80°C, but survival rates of SC and SD were much higher (~1,000 times) than those of SF at 90°C. However, germination capacity of SF was 20% higher than those of SD and SC on Luria-Bertani agar plates for 24 h. SF germinated more rapidly in a liquid medium with high NaCl concentrations than SC and SD, but became slower under alkaline conditions. Raman spectroscopy was used to analyze the heterogeneities in the three types of vegetative cells and their spores under different nutritional conditions. Exponentially grown-each vegetative cells had different overall Raman peak values. Raman peaks of SC, SD, and SF also showed differences in adenine and amide III compositions and nucleic acid contents. Our data along with Raman spectroscopy provided the evidence that spores formed under under different growth conditions possess very different cellular components, which affected their survival and germination rates.

Citations

Citations to this article as recorded by

Characterization of the Bacillus cereus spore killed by plasma-activated water (PAW)
Xiao Hu, Pengfei Ge, Xiaomeng Wang, Xinyu Liao, Jinsong Feng, Ruiling Lv, Tian Ding
Food Research International.2024; 196: 115058. CrossRef
Alleviation of H2O2 toxicity by extracellular catalases in the phycosphere of Microcystis aeruginosa
Yerim Park, Wonjae Kim, Yeji Cha, Minkyung Kim, Woojun Park
Harmful Algae.2024; 137: 102680. CrossRef
Effects of sporulation conditions on the growth, germination, and resistance of Clostridium perfringens spores
Dong Liang, Xiaoshuang Cui, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Shijie Liu, Gaiming Zhao, Na Wang, Yangyang Ma, Lina Xu
International Journal of Food Microbiology.2023; 396: 110200. CrossRef
Lysinibacilli: A Biological Factories Intended for Bio-Insecticidal, Bio-Control, and Bioremediation Activities
Qazi Mohammad Sajid Jamal, Varish Ahmad
Journal of Fungi.2022; 8(12): 1288. CrossRef
Discrimination of Stressed and Non-Stressed Food-Related Bacteria Using Raman-Microspectroscopy
Daniel Klein, René Breuch, Jessica Reinmüller, Carsten Engelhard, Peter Kaul
Foods.2022; 11(10): 1506. CrossRef
Detection of low numbers of bacterial cells in a pharmaceutical drug product using Raman spectroscopy and PLS-DA multivariate analysis
R. A. Grosso, A. R. Walther, E. Brunbech, A. Sørensen, B. Schebye, K. E. Olsen, H. Qu, M. A. B. Hedegaard, E. C. Arnspang
The Analyst.2022; 147(15): 3593. CrossRef
Linkage between bacterial community-mediated hydrogen peroxide detoxification and the growth of Microcystis aeruginosa
Minkyung Kim, Wonjae Kim, Yunho Lee, Woojun Park
Water Research.2021; 207: 117784. CrossRef

Functional and structural characterization of Deinococcus radiodurans R1 MazEF toxin-antitoxin system, Dr0416-Dr0417: Immanuel Dhanasingh , Eunsil Choi , Jeongeun Lee , Sung Haeng Lee , Jihwan Hwang; J. Microbiol. 2021;59(2):186-201. Published online February 1, 2021; DOI: https://doi.org/10.1007/s12275-021-0523-z

347 View
0 Download
6 Web of Science
4 Crossref

Abstract PDF

In prokaryotes, toxin-antitoxin (TA) systems are commonly found. They likely reflect the adaptation of pathogenic bacteria or extremophiles to various unfavorable environments by slowing their growth rate. Genomic analysis of the extremophile Deinococcus radiodurans R1 revealed the presence of eight type II TA systems, including the genes dr0417, dr0660, dr1530, dr0690, and dr1807. Expression of these toxin genes led to inhibition of Escherichia coli growth, whereas their antidote antitoxins were able to recover the growth defect. Remarkably, Dr0417 (DrMazF) showed endoribonuclease activity toward rRNAs as well as mRNAs, as determined by in vivo and in vitro RNA cleavage assays, and this activity was inhibited by Dr0416 (DrMazE). It was also found that the expression of dr0416-0417 module is directly regulated by the DrMazE-MazF complex. Furthermore, this TA module was induced under stress conditions and plays an important role in survival. To understand the regulatory mechanism at the molecular level, we determined the first high-resolution structures of DrMazF alone and of the DrMazE-MazF complex. In contrast with the hetero-hexameric state of typical MazEMazF complexes found in other species, DrMazE-MazF crystal structure consists of a hetero-trimer, with the DNA-binding domain of DrMazE undergoing self-cleavage at the flexible linker loop. Our structure revealed that the unique residue R54 provides an additional positive charge to the substratebinding pocket of DrMazF, its mutation significantly affects the endonuclease activity. Thus, our work reports the unique structural and biochemical features of the DrMazE-MazF system.

Citations

Citations to this article as recorded by

SeaMoon: From protein language models to continuous structural heterogeneity
Valentin Lombard, Dan Timsit, Sergei Grudinin, Elodie Laine
Structure.2025; 33(9): 1577. CrossRef
Focused Overview of Mycobacterium tuberculosis VapBC Toxin–Antitoxin Systems Regarding Their Structural and Functional Aspects: Including Insights on Biomimetic Peptides
Sung-Min Kang
Biomimetics.2023; 8(5): 412. CrossRef
Functional characterization of HigBA toxin-antitoxin system in an Arctic bacterium, Bosea sp. PAMC 26642
Eunsil Choi, Ahhyun Huh, Changmin Oh, Jeong-Il Oh, Ho Young Kang, Jihwan Hwang
Journal of Microbiology.2022; 60(2): 192. CrossRef
Identification and characterization of the type II toxin-antitoxin systems in the carbapenem-resistant Acinetobacter baumannii
Alireza Japoni-Nejad, Elnaz Harifi Mood, Parastoo Ehsani, Soroush Sardari, Fatemah Sadeghpour Heravi, Saeid Bouzari, Nader Shahrokhi
Microbial Pathogenesis.2021; 158: 105052. CrossRef

Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense: Syahriel Abdullah , Yoon Sin Oh , Min-Kyu Kwak , KhimPhin Chong; J. Microbiol. 2021;59(2):164-174. Published online December 23, 2020; DOI: https://doi.org/10.1007/s12275-021-0551-8

359 View
0 Download
11 Web of Science
11 Crossref

Abstract PDF

There have been relatively few studies which support a link between Ganoderma boninense, a phytopathogenic fungus that is particularly cytotoxic and pathogenic to plant tissues and roots, and antimicrobial compounds. We previously observed that liquid-liquid extraction (LLE) using chloroformmethanol- water at a ratio (1:1:1) was superior at detecting antibacterial activities and significant quantities of antibacterial compounds. Herein, we demonstrate that antibacterial secondary metabolites are produced from G. boninense mycelia. Antibacterial compounds were monitored in concurrent biochemical and biophysical experiments. The combined
methods
included high performance thin-layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR) spectroscopy. The antibacterial compounds derived from mycelia with chloroform-methanol extraction through LLE were isolated via a gradient solvent elution system using HPTLC. The antibacterial activity of the isolated compounds was observed to be the most potent against Staphylococcus aureus ATCC 25923 and multidrug-resistant S. aureus NCTC 11939. GC-MS, HPLC, and FTIR analysis confirmed two antibacterial compounds, which were identified as 4,4,14α-trimethylcholestane (m/z = 414.75; lanostane, C30H54) and ergosta-5,7,22-trien-3β-ol (m/z = 396.65; ergosterol, C28H44O). With the aid of spectroscopic evaluations, ganoboninketal (m/z = 498.66, C30H42O6), which belongs to the 3,4-seco-27-norlanostane triterpene family, was additionally characterized by 2D-NMR analysis. Despite the lack of antibacterial potential exhibited by lanostane; both ergosterol and ganoboninketal displayed significant antibacterial activities against bacterial pathogens. Results provide evidence for the existence of bioactive compounds in the mycelia of the relatively unexplored phytopathogenic G. boninense, together with a robust method for estimating the corresponding potent antibacterial secondary metabolites.

Citations

Citations to this article as recorded by

Techno‐Functional Biochemical Analysis and Food Applications of Edible Mushroom Powder
Subhra De, Prince Chawla, Sanju Bala Dhull, Gulden Goksen, Anarase Dattatray Arjun, Aarti Bains, Nadica Maltar Strmečki
Journal of Food Biochemistry.2025;[Epub] CrossRef
Optimization of Ganoderma lingzhi triterpene extraction method and its hypoglycemic activity
Shuang Hua, Yanshuang Li, Feng Jin, Meiyao Gan, Xianshun Jiang, Ying Zhang, Bo Zhang, Xiao Li
Preparative Biochemistry & Biotechnology.2025; 55(9): 1180. CrossRef
Exploring the health benefits of Ganoderma: antimicrobial properties and mechanisms of action
Samantha C. Karunarathna, Nimesha M. Patabendige, Kalani K. Hapuarachchi, Itthayakorn Promputtha
Frontiers in Cellular and Infection Microbiology.2025;[Epub] CrossRef
Extraction, Isolation and Characterization of Bioactive Compounds from Euphorbia neriifolia (L.) Leaf and Evaluation of their Antioxidant Activity
Priya Chaudhary, Devendra Singh, Mukesh Meena, Pracheta Janmeda
Current Analytical Chemistry.2025; 21(5): 559. CrossRef
Anti-Staphylococcus aureus potential of compounds from Ganoderma sp.: A comprehensive molecular docking and simulation approaches
Trang Thi Thu Nguyen, Trinh Thi Tuyet Nguyen, Hoang Duc Nguyen, Tan Khanh Nguyen, Phu Tran Vinh Pham, Linh Thuy Thi Tran, Hong Khuyen Thi Pham, Phu Chi Hieu Truong, Linh Thuoc Tran, Manh Hung Tran
Heliyon.2024; 10(7): e28118. CrossRef
Medium composition optimization and characterization of polysaccharides extracted from Ganoderma boninense along with antioxidant activity
Qian-Zhu Li, Chuan Xiong, Wei Chee Wong, Li-Wei Zhou
International Journal of Biological Macromolecules.2024; 260: 129528. CrossRef
Plant Defense Inducers and Antioxidant Metabolites Produced During Oil Palm-Ganoderma boninense Interaction In Vitro
Neda Shokrollahi, Chai-Ling Ho, Nur Ain Izzati Mohd Zainudin, Mohd As’wad Bin Abdul Wahab, Mui-Yun Wong
Chemistry Africa.2023; 6(1): 499. CrossRef
Identification of Antibacterial Metabolites from Endophytic Fungus Aspergillus fumigatus, Isolated from Albizia lucidior Leaves (Fabaceae), Utilizing Metabolomic and Molecular Docking Techniques
Mai E. Hussein, Osama G. Mohamed, Ahlam M. El-Fishawy, Hesham I. El-Askary, Amira S. El-Senousy, Ahmed A. El-Beih, Eman S. Nossier, Ahmed M. Naglah, Abdulrahman A. Almehizia, Ashootosh Tripathi, Ahmed A. Hamed
Molecules.2022; 27(3): 1117. CrossRef
Bioactive Compounds of Ganoderma boninense Inhibited Methicillin-Resistant Staphylococcus aureus Growth by Affecting Their Cell Membrane Permeability and Integrity
Yow-San Chan, Khim-Phin Chong
Molecules.2022; 27(3): 838. CrossRef
Review Update on the Life Cycle, Plant–Microbe Interaction, Genomics, Detection and Control Strategies of the Oil Palm Pathogen Ganoderma boninense
Izwan Bharudin, Anis Farhan Fatimi Ab Wahab, Muhammad Asyraff Abd Samad, Ng Xin Yie, Madihah Ahmad Zairun, Farah Diba Abu Bakar, Abdul Munir Abdul Murad
Biology.2022; 11(2): 251. CrossRef
Screening for Antibacterial Activity of French Mushrooms against Pathogenic and Multidrug Resistant Bacteria
Clément Huguet, Mélanie Bourjot, Jean-Michel Bellanger, Gilles Prévost, Aurélie Urbain
Applied Sciences.2022; 12(10): 5229. CrossRef

Genetic linkage map construction and quantitative trait loci mapping of agronomic traits in Gloeostereum incarnatum: Wan-Zhu Jiang , Fang-Jie Yao , Li-Xin Lu , Ming Fang , Peng Wang , You-Min Zhang , Jing-Jing Meng , Jia Lu , Xiao-Xu Ma , Qi He , Kai-Sheng Shao; J. Microbiol. 2021;59(1):41-50. Published online November 17, 2020; DOI: https://doi.org/10.1007/s12275-021-0242-5

324 View
0 Download
7 Web of Science
7 Crossref

Abstract PDF

Gloeostereum incarnatum is an edible medicinal mushroom widely grown in China. Using the whole genome of G. incarnatum, simple sequence repeat (SSR) markers were developed and synthetic primers were designed to construct its first genetic linkage map. The 1,048.6 cm map is composed of 10 linkage groups and contains 183 SSR markers. In total, 112 genome assembly sequences were anchored, representing 16.43 Mb and covering 46.41% of the genome. Selfing populations were used for quantitative trait loci (QTL) targeting, and the composite interval mapping method was used to co-localize the mycelium growth rate (potato dextrose agar and sawdust), growth period, yield and fruiting body length, and width and thickness. The 14 QTLs of agronomic traits had LOD values of 3.20–6.51 and contribution rates of 2.22– 13.18%. No linkage relationship was found between the mycelium growth rate and the growth period, but a linkage relationship was observed among the length, width and thickness of the fruiting bodies. Using NCBI’s BLAST alignment, the genomic sequences corresponding to the QTL regions were compared, and a TPR-like protein candidate gene was selected. Using whole-genome data, 138 candidate genes were found in four sequence fragments of two SSR markers located in the same scaffold. The genetic map and QTLs established in this study will aid in developing selective markers for agronomic traits and identifying corresponding genes, thereby providing a scientific basis for the further gene mapping of quantitative traits and the marker-assisted selection of functional genes in G. incarnatum breeding programs.

Citations

Citations to this article as recorded by

Biochemistry and Chemoinformatics Guided Classification of Hirsutane Sesquiterpenes Isolated from Mushroom
Yaping Liu, Jumpei Nishishita, Chengwei Liu, Hideaki Oikawa, Atsushi Minami
JACS Au.2025; 5(2): 740. CrossRef
Dicarboxylic Amino Acid Permease 7219 Regulates Fruiting Body Type of Auricularia heimuer
Jia Lu, Lixin Lu, Fangjie Yao, Ming Fang, Xiaoxu Ma, Jingjing Meng, Kaisheng Shao
Journal of Fungi.2023; 9(9): 876. CrossRef
Identification of quantitative trait loci for growth traits in red swamp crayfish (Procambarus clarkii)
Junxiao Sun, Cuirong Luo, Bo Peng, Guohui Peng, Yunfei Tan, Xufeng Bai
Aquaculture and Fisheries.2023; 8(6): 727. CrossRef
Improved Genetic Map and Localization of Quantitative Trait Loci for Quality Traits in Auricularia heimuer
Jia Lu, Ming Fang, Fangjie Yao, Lixin Lu, Xiaoxu Ma, Jingjing Meng, Kaisheng Shao
Horticulturae.2023; 9(7): 763. CrossRef
Assemblies of the genomes of parasitic wasps using meta-assembly and scaffolding with genetic linkage
Kameron T Wittmeyer, Sara J Oppenheim, Keith R Hopper, J Hesselberth
G3 Genes|Genomes|Genetics.2022;[Epub] CrossRef
EST-SSR Primer Development and Genetic Structure Analysis of Psathyrostachys juncea Nevski
Zhen Li, Lan Yun, Zhiqi Gao, Tian Wang, Xiaomin Ren, Yan Zhao
Frontiers in Plant Science.2022;[Epub] CrossRef
Analysis of the Genome Sequence of Strain GiC-126 of Gloeostereum incarnatum with Genetic Linkage Map
Wan-Zhu Jiang, Fang-Jie Yao, Ming Fang, Li-Xin Lu, You-Min Zhang, Peng Wang, Jing-Jing Meng, Jia Lu, Xiao-Xu Ma, Qi He, Kai-Sheng Shao, Asif Ali Khan, Yun-Hui Wei
Mycobiology.2021; 49(4): 406. CrossRef

[PROTOCOL]A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans: Kwang-Woo Jung , Kyung-Tae Lee , Yong-Sun Bahn; J. Microbiol. 2020;58(10):823-831. Published online September 29, 2020; DOI: https://doi.org/10.1007/s12275-020-0341-8

366 View
0 Download
2 Web of Science
2 Crossref

Abstract PDF

Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen in its host. Recently, this
method
has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM
method
for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence.

Citations

Citations to this article as recorded by

Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
Dayoung Sung, Garam Choi, Minji Ahn, Hokyung Byun, Tae Young Kim, Hojun Lee, Zee-Won Lee, Ji Yong Park, Young Hyun Jung, Ho Jae Han, Sang Ho Choi
Nucleic Acids Research.2025;[Epub] CrossRef
Zinc-binding domain mediates pleiotropic functions of Yvh1 in Cryptococcus neoformans
Jae-Hyung Jin, Myung Kyung Choi, Hyun-Soo Cho, Yong-Sun Bahn
Journal of Microbiology.2021; 59(7): 658. CrossRef

Caspase-3 inhibitor inhibits enterovirus D68 production: Wenbo Huo , Jinghua Yu , Chunyu Liu , Ting Wu , Yue Wang , Xiangling Meng , Fengmei Song , Shuxia Zhang , Ying Su , Yumeng Liu , Jinming Liu , Xiaoyan Yu , Shucheng Hua; J. Microbiol. 2020;58(9):812-820. Published online September 1, 2020; DOI: https://doi.org/10.1007/s12275-020-0241-y

361 View
0 Download
9 Web of Science
9 Crossref

Abstract PDF

Enterovirus D68 (EVD68) is an emerging pathogen that recently caused a large worldwide outbreak of severe respiratory disease in children. However, the relationship between EVD68 and host cells remains unclear. Caspases are involved in cell death, immune response, and even viral production. We found that caspase-3 was activated during EVD68 replication to induce apoptosis. Caspase-3 inhibitor (Z-DEVDFMK) inhibited viral production, protected host cells from the cytopathic effects of EVD68 infection, and prevented EVD68 from regulating the host cell cycle at G0/G1. Meanwhile, caspase-3 activator (PAC-1) increased EVD68 production. EVD68 infection therefore activates caspase-3 for virus production. This knowledge provides a potential direction for the prevention and treatment of disease related to EVD68.

Citations

Citations to this article as recorded by

Non-Polio Enterovirus Inhibitors: Scaffolds, Targets, and Potency─What’s New?
Hugo Fernando Georges Roux, Franck Touret, Pascal Rathelot, Pietro Sciò, Antonio Coluccia, Patrice Vanelle, Manon Roche
ACS Infectious Diseases.2025; 11(1): 21. CrossRef
Mechanistic and target study of anthraquinone modifier KA-4c triggering endoplasmic reticulum stress to inhibit triple-negative breast cancer cells
Yingying Yang, Shumei Xu, Qiangjian Chen, Yingdan Zhao, Xinxiao Li, Junying Li, Huaxin Hou, Danrong Li
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease.2025; 1871(8): 168020. CrossRef
Mode of cell death in the penile cavernous tissue of type 1 diabetes mellitus rats
Jing Li, Qilan Jiang, Jun Jiang, Rui Jiang
The Journal of Sexual Medicine.2024; 21(8): 652. CrossRef
MDA5 Enhances Invasive Candida albicans Infection by Regulating Macrophage Apoptosis and Phagocytosis/Killing Functions
Yayun Chen, Qian Jiang, Furong Qing, Junxia Xue, Qiuxiang Xiao, Wenji He, Lina Sui, Zhiping Liu
Inflammation.2024; 47(1): 191. CrossRef
Caspase-8 activation regulates enterovirus D68 infection-induced inflammatory response and cell death
Yuanyuan Zhou, Chongtao Zhang, Yuhan Zhang, Fei Li, Jun Shen
Biosafety and Health.2024; 6(3): 171. CrossRef
Enterovirus D68 Infection Induces TDP-43 Cleavage, Aggregation, and Neurotoxicity
Lili Zhang, Jiaxin Yang, Huili Li, Zhe Zhang, Zhilin Ji, Lirong Zhao, Wei Wei, Rebecca Ellis Dutch
Journal of Virology.2023;[Epub] CrossRef
Inhibitory effect of tanshinone IIA, resveratrol and silibinin on enterovirus 68 production through inhibiting ATM and DNA-PK pathway
Ying Su, Ting Wu, Xiao-Yan Yu, Wen-Bo Huo, Shao-Hua Wang, Chen Huan, Yu-Meng Liu, Jin-Ming Liu, Min-Na Cui, Xin-Hua Li, Jing-Hua Yu
Phytomedicine.2022; 99: 153977. CrossRef
Urolithin A inhibits enterovirus 71 replication and promotes autophagy and apoptosis of infected cells in vitro
Shengyu Wang, Junhua Qiao, Yaping Chen, Langfei Tian, Xin Sun
Archives of Virology.2022; 167(10): 1989. CrossRef
Mst1/2-ALK promotes NLRP3 inflammasome activation and cell apoptosis during Listeria monocytogenes infection
Aijiao Gao, Huixin Tang, Qian Zhang, Ruiqing Liu, Lin Wang, Yashan Liu, Zhi Qi, Yanna Shen
Journal of Microbiology.2021; 59(7): 681. CrossRef

Review

Recent advances in the development of β-lactamase inhibitors: Shivakumar S. Jalde , Hyun Kyung Choi; J. Microbiol. 2020;58(8):633-647. Published online July 27, 2020; DOI: https://doi.org/10.1007/s12275-020-0285-z

339 View
1 Download
23 Web of Science
22 Crossref

Abstract PDF

β-Lactam antibiotics are the most commonly prescribed antibiotics worldwide; however, antimicrobial resistance (AMR) is a global challenge. The β-lactam resistance in Gram-negative bacteria is due to the production of β-lactamases, including extended-spectrum β-lactamases, metallo-β-lactamases, and carbapenem-hydrolyzing class D β-lactamases. To restore the efficacy of BLAs, the most successful strategy is to use them in combination with β-lactamase inhibitors (BLI). Here we review the medically relevant β-lactamase families and penicillins, diazabicyclooctanes, boronic acids, and novel chemical scaffold-based BLIs, in particular approved and under clinical development.

Citations

Citations to this article as recorded by

Functional and structural analyses of IMP-27 metallo-β-lactamase: evolution of IMP-type enzymes to overcome Zn(II) deprivation
Yoshiki Kato, Toshio Yamaguchi, Haruka Nakagawa-Kamura, Yoshikazu Ishii, Akiko Shimizu-Ibuka, Pablo Power
Microbiology Spectrum.2024;[Epub] CrossRef
Current Strategy for Targeting Metallo-β-Lactamase with Metal-Ion-Binding Inhibitors
Jessica L. Ortega-Balleza, Lenci K. Vázquez-Jiménez, Eyra Ortiz-Pérez, Guadalupe Avalos-Navarro, Alma D. Paz-González, Edgar E. Lara-Ramírez, Gildardo Rivera
Molecules.2024; 29(16): 3944. CrossRef
Understanding the Functional Dynamics of the TokK Enzyme in Carbapenem Biosynthesis via MD Simulations and QM/MM Calculations
Shakir Ali Siddiqui, Kshatresh Dutta Dubey
Inorganic Chemistry.2024; 63(40): 18963. CrossRef
Recent advances in functionalized macrocyclic polyamines for medicine applications
Hao Chang, Renzhong Qiao, Chao Li
Chinese Chemical Letters.2024; : 110675. CrossRef
Exploring the dynamics of gut microbiota, antibiotic resistance, and chemotherapy impact in acute leukemia patients: A comprehensive metagenomic analysis
Ying Luo, Taha Majid Mahmood Sheikh, Xin Li, YuMeng Yuan, Fen Yao, Meimei Wang, Xiaoling Guo, Jilong Wu, Muhammad Shafiq, Qingdong Xie, Xiaoyang Jiao
Virulence.2024;[Epub] CrossRef
Decrypting biocontrol functions and application modes by genomes data of three Trichoderma Strains/Species
Shida Ji, Bin Liu, Jing Han, Ning Kong, Yongfeng Yang, Yucheng Wang, Zhihua Liu
Fungal Genetics and Biology.2024; 172: 103889. CrossRef
Revisiting the Checkerboard to Inform Development of β-Lactam/β-Lactamase Inhibitor Combinations
Darren J. Bentley
Antibiotics.2024; 13(4): 337. CrossRef
Role of β-Lactamase Inhibitors as Potentiators in Antimicrobial Chemotherapy Targeting Gram-Negative Bacteria
Song Zhang, Xinyu Liao, Tian Ding, Juhee Ahn
Antibiotics.2024; 13(3): 260. CrossRef
The C5α-Methyl-Substituted Carbapenem NA-1-157 Exhibits Potent Activity against Klebsiella spp. Isolates Producing OXA-48-Type Carbapenemases
Clyde A. Smith, Nichole K. Stewart, Marta Toth, Pojun Quan, John D. Buynak, Sergei B. Vakulenko
ACS Infectious Diseases.2023; 9(5): 1123. CrossRef
Phenotypes, genotypes and breakpoints: an assessment of β-lactam/β-lactamase inhibitor combinations against OXA-48
Tomefa E Asempa, Abigail K Kois, Christian M Gill, David P Nicolau
Journal of Antimicrobial Chemotherapy.2023; 78(3): 636. CrossRef
Characteristics of Extended-Spectrum β-Lactamase-Producing Escherichia coli Derived from Food and Humans in Northern Xinjiang, China
Yushuang Wu, Shudi Huang, Donglai Zhang, Hua Ji, Yongqing Ni, Xueling Zhang, Juan Dong, Baokun Li
Foodborne Pathogens and Disease.2023; 20(7): 270. CrossRef
Sequential C−H Methylation Catalyzed by the B12‐Dependent SAM Enzyme TokK: Comprehensive Theoretical Study of Selectivities
Wen‐Hao Deng, Rong‐Zhen Liao
Chemistry – A European Journal.2023;[Epub] CrossRef
CMOS Spectrophotometric Microsystem for Malaria Detection
Gabriel M. Ferreira, Vitória Baptista, Vítor Silva, Maria I. Veiga, Graça Minas, Susana O. Catarino
IEEE Transactions on Biomedical Engineering.2023; 70(8): 2318. CrossRef
Synthesis and β-Lactamase Inhibition Activity of Imidates of Diazabicyclooctane
Lijuan Zhai, Jian Sun, Jingwen Ji, Lili He, Yuanyu Gao, Jinbo Ji, Yuanbai Liu, Yangxiu Mu, Xueqin Ma, Dong Tang, Haikang Yang, Zafar Iqbal, Zhixiang Yang
Russian Journal of Bioorganic Chemistry.2022; 48(5): 1059. CrossRef
Recent Developments to Cope the Antibacterial Resistance via β-Lactamase Inhibition
Zafar Iqbal, Jian Sun, Haikang Yang, Jingwen Ji, Lili He, Lijuan Zhai, Jinbo Ji, Pengjuan Zhou, Dong Tang, Yangxiu Mu, Lin Wang, Zhixiang Yang
Molecules.2022; 27(12): 3832. CrossRef
Retracted and replaced: Phenotypes, genotypes and breakpoints: an assessment of β-lactam/ β-lactamase inhibitor combinations against OXA-48
Tomefa E Asempa, Abigail K Kois, Christian M Gill, David P Nicolau
Journal of Antimicrobial Chemotherapy.2022; 77(10): 2622. CrossRef
Carbapenemase producing Klebsiella pneumoniae: implication on future therapeutic strategies
Ilias Karaiskos, Irene Galani, Vassiliki Papoutsaki, Lamprini Galani, Helen Giamarellou
Expert Review of Anti-infective Therapy.2022; 20(1): 53. CrossRef
Antimicrobial Activity of Dihydroisocoumarin Isolated from Wadi Lajab Sediment-Derived Fungus Penicillium chrysogenum: In Vitro and In Silico Study
Raha Orfali, Shagufta Perveen, Mohamed Fahad AlAjmI, Safina Ghaffar, Md Tabish Rehman, Abdullah R. AlanzI, Saja Bane Gamea, Mona Essa Khwayri
Molecules.2022; 27(11): 3630. CrossRef
The Odd Couple(s): An Overview of Beta-Lactam Antibiotics Bearing More Than One Pharmacophoric Group
Margherita De Rosa, Anna Verdino, Annunziata Soriente, Anna Marabotti
International Journal of Molecular Sciences.2021; 22(2): 617. CrossRef
Drugs That Changed Society: History and Current Status of the Early Antibiotics: Salvarsan, Sulfonamides, and β-Lactams
Søren Brøgger Christensen
Molecules.2021; 26(19): 6057. CrossRef
In Crystallo Time-Resolved Interaction of the Clostridioides difficile CDD-1 enzyme with Avibactam Provides New Insights into the Catalytic Mechanism of Class D β-lactamases
Nichole K. Stewart, Marta Toth, Anastasiya Stasyuk, Sergei B. Vakulenko, Clyde A. Smith
ACS Infectious Diseases.2021; 7(6): 1765. CrossRef
Inhibition of the Clostridioides difficile Class D β-Lactamase CDD-1 by Avibactam
Nichole K. Stewart, Marta Toth, Anastasiya Stasyuk, Mijoon Lee, Clyde A. Smith, Sergei B. Vakulenko
ACS Infectious Diseases.2021; 7(5): 1164. CrossRef

Journal Articles

Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulatessensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology: Kui Wu , Yangyun Zheng , Qingping Wu , Haiying Chen , Songzhe Fu , Biao Kan , Yongyan Long , Xiansheng Ni , Junling Tu; J. Microbiol. 2019;57(12):1105-1114. Published online November 4, 2019; DOI: https://doi.org/10.1007/s12275-019-9379-x

302 View
0 Download
12 Web of Science
11 Crossref

Abstract PDF

In order to adapt to different environments, Vibrio parahaemolyticus employed a complicated quorum sensing system to orchestrate gene expression and diverse colony morphology patterns. In this study, the function of the putative quorum sensing signal synthase gene cqsA (VPA0711 in V. parahaemolyticus strain RIMD2210633 genome) was investigated. The cloning and expression of V. parahaemolyticus cqsA in Escherichia coli system induced the production of a new quorum sensing signal that was found in its culture supernatant. The signal was purified by high performance liquid chromatography
methods
and determined to be 3-hydroxyundecan- 4-one by indirect and direct mass spectra assays. The deletion of cqsA in RIMD2210633 changed V. parahaemolyticus colony morphology from the classical ‘fried-egg’ shape (thick and opaque in the center, while thin and translucent in the edge) of the wild-type colony to a ‘pancake’ shape (no significant difference between the centre and the edge) of the cqsAdeleted colony. This morphological change could be restored by complementary experiment with cqsA gene or the signal extract. In addition, the expression of opaR, a well-known quorum sensing regulatory gene, could be up-regulated by cqsA deletion. Our results suggested that V. parahaemolyticus used cqsA to produce 3-hydroxyundecan-4-one signal and thereby regulated colony morphology and other quorum sensing-associated behaviors.

Citations

Citations to this article as recorded by

Antimicrobial resistance, virulence factors and phylogenetic profiles of Vibrio parahaemolyticus in the eastern coast of Shenzhen
Xian Qiang Lian, Guo Dong Liu, Miao Fen Huang, Qiu Hua Fan, Zi Dan Lin
Frontiers in Microbiology.2024;[Epub] CrossRef
Quorum sensing signal synthases enhance Vibrio parahaemolyticus swarming motility
Fuwen Liu, Fei Wang, Yixuan Yuan, Xiaoran Li, Xiaojun Zhong, Menghua Yang
Molecular Microbiology.2023; 120(2): 241. CrossRef
Regulation of Virulence Factors Expression During the Intestinal Colonization of Vibrio parahaemolyticus
Jingyu Wang, Yuming Zhan, Han Sun, Xiaodan Fu, Qing Kong, Changliang Zhu, Haijin Mou
Foodborne Pathogens and Disease.2022; 19(3): 169. CrossRef
Supplementation of ex situ produced bioflocs improves immune response against AHPND in Pacific whiteleg shrimp (Litopenaeus vannamei) postlarvae
Magdalena Lenny Situmorang, Umaporn Uawisetwathana, Sopacha Arayamethakorn, Nitsara Karoonuthaisiri, Wanilada Rungrassamee, Haniswita Haniswita, Peter Bossier, Gede Suantika
Applied Microbiology and Biotechnology.2022; 106(9-10): 3751. CrossRef
A novel finding of intra-genus inhibition of quorum sensing in Vibrio bacteria
Huong Thanh Hoang, Thuy Thu Thi Nguyen, Ha Minh Do, Thao Kim Nu Nguyen, Hai The Pham
Scientific Reports.2022;[Epub] CrossRef
CqsA-introduced quorum sensing inhibits type VI secretion system 2 through an OpaR-dependent pathway in Vibrio parahaemolyticus
Kui Wu, Yongyan Long, Qian Liu, Wei Wang, Guoyin Fan, Hui Long, Yangyun Zheng, Xiansheng Ni, Shengen Chen, Haiying Chen, Shufen Shuai
Microbial Pathogenesis.2022; 162: 105334. CrossRef
CqsA inhibits the virulence of Vibrio harveyi to the pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)
Yaqiu Zhang, Yiqin Deng, Juan Feng, Zhixun Guo, Can Mao, Haoxiang Chen, Ziyang Lin, Jianmei Hu, Youlu Su
Aquaculture.2021; 535: 736346. CrossRef
Identification of LuxR Family Regulators That Integrate Into Quorum Sensing Circuit in Vibrio parahaemolyticus
Xiaojun Zhong, Ranran Lu, Fuwen Liu, Jinjie Ye, Junyang Zhao, Fei Wang, Menghua Yang
Frontiers in Microbiology.2021;[Epub] CrossRef
Adaptations of Vibrio parahaemolyticus to Stress During Environmental Survival, Host Colonization, and Infection
Gururaja Perumal Pazhani, Goutam Chowdhury, Thandavarayan Ramamurthy
Frontiers in Microbiology.2021;[Epub] CrossRef
Vibrio alginolyticus influences quorum sensing-controlled phenotypes of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus
Panida Paopradit, Natta Tansila, Komwit Surachat, Pimonsri Mittraparp-arthorn
PeerJ.2021; 9: e11567. CrossRef
Dynamics and Microevolution of Vibrio parahaemolyticus Populations in Shellfish Farms
Songzhe Fu, Qingyao Wang, Yixiang Zhang, Qian Yang, Jingwei Hao, Ying Liu, Bo Pang, Michael S. Rappe
mSystems.2021;[Epub] CrossRef

FgIlv3a is crucial in branched-chain amino acid biosynthesis, vegetative differentiation, and virulence in Fusarium graminearum: Xin Liu , Yichen Jiang , Yinghui Zhang , Mingzheng Yu , Hongjun Jiang , Jianhong Xu , Jianrong Shi; J. Microbiol. 2019;57(8):694-703. Published online May 11, 2019; DOI: https://doi.org/10.1007/s12275-019-9123-6

331 View
0 Download
15 Web of Science
11 Crossref

Abstract PDF

Dihydroxyacid dehydratase (DHAD), encoded by ILV3, catalyses the third step in the biosynthetic pathway of branchedchain amino acids (BCAAs), which include isoleucine (Ile), leucine (Leu), and valine (Val). Enzymes involved in BCAA biosynthesis exist in bacteria, plants, and fungi but not in mammals and are therefore attractive targets for antimicrobial or herbicide development. In this study, three paralogous ILV3 genes (FgILV3A, FgILV3B, and FgILV3C) were identified in the genome of Fusarium graminearum, the causal agent of Fusarium head blight (FHB). Deletion of FgILV3A alone or combined with FgILV3B or FgILV3C indicated an important role for FgILV3A in BCAA biosynthesis. FgILV3A deletion mutants lost the ability to grow on medium lacking amino acids. Exogenous supplementation of 1 mM Ile and Val rescued the auxotrophy of ΔFgIlv3A, though 5 mM was required to recover the growth defects in ΔFgIlv3AB and ΔFgIlv3AC strains, indicating that FgIlv3b and FgIlv3c exhibit redundant but accessory roles with FgIlv3a in BCAA biosynthesis. The auxotrophy of ΔFgIlv3A resulted in pleiotropic defects in aerial hyphal growth, in conidial formation and germination, and in aurofusarin accumulation. In addition, the mutants showed reduced virulence and deoxynivalenol production. Overall, our study demonstrates that FgIlv3a is crucial for BCAA biosynthesis in F. graminearum and a candidate fungicide target for FHB management.

Citations

Citations to this article as recorded by

AflaILVB/G/I and AflaILVD are involved in mycelial production, aflatoxin biosynthesis, and fungal virulence in Aspergillus flavus
Yarong Zhao, Chulan Huang, Rui Zeng, Peirong Chen, Kaihang Xu, Xiaomei Huang, Xu Wang
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Histone H3 N-Terminal Lysine Acetylation Governs Fungal Growth, Conidiation, and Pathogenicity through Regulating Gene Expression in Fusarium pseudograminearum
Hang Jiang, Lifang Yuan, Liguo Ma, Kai Qi, Yueli Zhang, Bo Zhang, Guoping Ma, Junshan Qi
Journal of Fungi.2024; 10(6): 379. CrossRef
Identification and Characterization of an Antifungal Gene Mt1 from Bacillus subtilis by Affecting Amino Acid Metabolism in Fusarium graminearum
Pei Song, Wubei Dong
International Journal of Molecular Sciences.2023; 24(10): 8857. CrossRef
Branched-chain amino acid biosynthesis in fungi
Gary Jones, Jane Usher, Joel T. Steyer, Richard B. Todd
Essays in Biochemistry.2023; 67(5): 865. CrossRef
FgLEU1 Is Involved in Leucine Biosynthesis, Sexual Reproduction, and Full Virulence in Fusarium graminearum
Shaohua Sun, Mingyu Wang, Chunjie Liu, Yilin Tao, Tian Wang, Yuancun Liang, Li Zhang, Jinfeng Yu
Journal of Fungi.2022; 8(10): 1090. CrossRef
Acetolactate synthases regulatory subunit and catalytic subunit genes VdILVs are involved in BCAA biosynthesis, microscletotial and conidial formation and virulence in Verticillium dahliae
ShengNan Shao, Biao Li, Qi Sun, PeiRu Guo, YeJuan Du, JiaFeng Huang
Fungal Genetics and Biology.2022; 159: 103667. CrossRef
Molecular targets for antifungals in amino acid and protein biosynthetic pathways
Aleksandra Kuplińska, Kamila Rząd
Amino Acids.2021; 53(7): 961. CrossRef
MoCpa1-mediated arginine biosynthesis is crucial for fungal growth, conidiation, and plant infection of Magnaporthe oryzae
Osakina Aron, Min Wang, Anjago Wilfred Mabeche, Batool Wajjiha, Meiqin Li, Shuai Yang, Haixia You, Yan Cai, Tian Zhang, Yunxi Li, Baohua Wang, Dongmei Zhang, Zonghua Wang, Wei Tang
Applied Microbiology and Biotechnology.2021; 105(14-15): 5915. CrossRef
Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid
Marta J. Fiołka, Paulina Czaplewska, Sylwia Wójcik-Mieszawska, Aleksandra Lewandowska, Kinga Lewtak, Weronika Sofińska-Chmiel, Tomasz Buchwald
Scientific Reports.2021;[Epub] CrossRef
The pyruvate dehydrogenase kinase 2 (PDK2) is associated with conidiation, mycelial growth, and pathogenicity in Fusarium graminearum
Tao Gao, Dan He, Xin Liu, Fang Ji, Jianhong Xu, Jianrong Shi
Food Production, Processing and Nutrition.2020;[Epub] CrossRef
The Intermediates in Branched-Chain Amino Acid Biosynthesis Are Indispensable for Conidial Germination of the Insect-Pathogenic Fungus Metarhizium robertsii
Feifei Luo, Hongxia Zhou, Xue Zhou, Xiangyun Xie, You Li, Fenglin Hu, Bo Huang, Karyn N. Johnson
Applied and Environmental Microbiology.2020;[Epub] CrossRef

Low-density lipoprotein as an opsonin promoting the phagocytosis of Pseudomonas aeruginosa by U937 cells: Yuxin Li , Zhi Liu , Jinli Yang , Ling Liu , Runlin Han; J. Microbiol. 2019;57(8):711-716. Published online May 11, 2019; DOI: https://doi.org/10.1007/s12275-019-8413-3

357 View
0 Download
2 Web of Science
2 Crossref

Abstract PDF

Low-density lipoprotein (LDL) was recently reported to be an opsonin, enhancing the phagocytosis of group A Streptococcus (GAS) by human monocytic leukemia U937 cells due to the binding of LDL to some GAS strains. We postulated that LDL might also promote the opsonophagocytosis of Pseudomonas aeruginosa by U937 cells since this bacterium interacts with LDL. In this study, P. aeruginosa (CMCC10104), U937 cells, and human LDL were used in phagocytosis assays to test our hypothesis. Escherichia coli strain BL21, which does not interact with LDL, was used as a negative control. Colony counting and fluorescence microscopy were used to determine the bacterial quantity in the opsonophagocytosis assays. After incubation of U937 cells and P. aeruginosa with LDL (100 μg/ml) for 15 and 30 min, phagocytosis was observed to be increased by 22.71% and 32.90%, respectively, compared to that seen in the LDL-free group. However, LDL did not increase the phagocytosis of E. coli by U937 cells. In addition, we identified CD36 as a major opsonin receptor on U937 cells, since an anti-CD36 monoclonal antibody, but not an anti- CD4 monoclonal antibody, almost completely abolished the opsonophagocytosis of P. aeruginosa by U937 cells.

Citations

Citations to this article as recorded by

Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glycoprotein 2
Christin Bartlitz, Rafał Kolenda, Jarosław Chilimoniuk, Krzysztof Grzymajło, Stefan Rödiger, Rolf Bauerfeind, Aamir Ali, Veronika Tchesnokova, Dirk Roggenbuck, Peter Schierack, Isaac Cann
Applied and Environmental Microbiology.2022;[Epub] CrossRef
Lipoprotein(a), an Opsonin, Enhances the Phagocytosis of Nontypeable Haemophilus influenzae by Macrophages
Zhi Liu, Yuxin Li, Yu Wang, Zhe Liu, Yan Su, Qiang Ma, Runlin Han, Enrique Ortega
Journal of Immunology Research.2021; 2021: 1. CrossRef

β-1,3-Glucan/CR3/SYK pathway-dependent LC3B-II accumulation enhanced the fungicidal activity in human neutrophils: Ding Li , Changsen Bai , Qing Zhang , Zheng Li , Di Shao , Xichuan Li; J. Microbiol. 2019;57(4):263-270. Published online February 5, 2019; DOI: https://doi.org/10.1007/s12275-019-8298-1

380 View
0 Download
9 Web of Science
7 Crossref

Abstract PDF

Since molecular genotyping has been established for the Candida species, studies have found that a single Candida strain (endemic strain) can persist over a long period of time and results in the spread of nosocomial invasive candidiasis without general characteristics of horizontal transmissions. Our previous study also found the existence of endemic strains in a cancer center in Tianjin, China. In the current study, we performed further investigation on endemic and non-endemic Candida albicans strains, with the aim of explaining the higher morbidity of endemic strains. In an in vivo experiment, mice infected with endemic strains showed significantly shorter survival time and higher kidney fungal burdens compared to mice infected with non-endemic strains. In an in vitro experiment, the killing percentage of neutrophils to endemic strains was significantly lower than that to non-endemic strains, which is positively linked to the ratio of LC3B-II/I in neutrophils. An immunofluorescence assay showed more β-1,3-glucan exposure on the cell walls of nonendemic strains compared to endemic strains. After blocking the β-glucan receptor (CR3) or inhibiting downstream kinase (SYK) in neutrophils, the killing percent to C. albicans (regardless of endemic and non-endemic strains) and the ratio of LC3B-II/I of neutrophils were significantly decreased. These data suggested that the killing capability of neutrophils to C. albicans was monitored by β-1,3-glucan via CR3/SYK pathway-dependent LC3B-II accumulation and provided an explanation for the variable killing capability of neutrophils to different strains of C. albicans, which would be beneficial in improving infection control and therapeutic strategies for invasive candidiasis.

Citations

Citations to this article as recorded by

LC3B: A microtubule-associated protein influences disease progression and prognosis
Yan Chen, Hong Yi, Shan Liao, Junyu He, Yanhong Zhou, Yan Lei
Cytokine & Growth Factor Reviews.2025; 81: 16. CrossRef
Autophagy and LC3-associated phagocytosis contribute negatively to the killing capability of THP-1-derived macrophages against Candida albicans at the mid-stage
Ding Li, Lin Wang, Zhihong Zhao, Changsen Bai, Xichuan Li
Immunology Letters.2023; 263: 25. CrossRef
Metabolism and Biodegradation of β-Glucan in vivo
Ziming Zheng, Wenqi Tang, Weipeng Lu, Xu Mu, Yuxuan Liu, Xianglin Pan, Kaiping Wang, Yu Zhang
Frontiers in Veterinary Science.2022;[Epub] CrossRef
Biological Effects of β-Glucans on Osteoclastogenesis
Wataru Ariyoshi, Shiika Hara, Ayaka Koga, Yoshie Nagai-Yoshioka, Ryota Yamasaki
Molecules.2021; 26(7): 1982. CrossRef
Interaction Between Dendritic Cells and Candida krusei β-Glucan Partially Depends on Dectin-1 and It Promotes High IL-10 Production by T Cells
Truc Thi Huong Dinh, Phawida Tummamunkong, Panuwat Padungros, Pranpariya Ponpakdee, Lawan Boonprakong, Wilasinee Saisorn, Asada Leelahavanichkul, Patipark Kueanjinda, Patcharee Ritprajak
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Insights on the Functional Role of Beta-Glucans in Fungal Immunity Using Receptor-Deficient Mouse Models
Mark Joseph Maranan Desamero, Soo-Hyun Chung, Shigeru Kakuta
International Journal of Molecular Sciences.2021; 22(9): 4778. CrossRef
Immunomodulatory Effect of Structurally Characterized Mushroom Sclerotial Polysaccharides Isolated from Polyporus rhinocerus on Bone Marrow Dendritic Cells
Chaoran Liu, Man Wing Choi, Xingkui Xue, Peter C. K. Cheung
Journal of Agricultural and Food Chemistry.2019; 67(43): 12137. CrossRef

Biosynthesis of 2-amino-3-hydroxycyclopent-2-enone moiety of bafilomycin in Kitasatospora cheerisanensis KCTC2395: Nguyen Phan Kieu Hanh , Jae Yoon Hwang , Hye Ryeung Oh , Geum Jin Kim , Hyukjae Choi , Doo Hyun Nam; J. Microbiol. 2018;56(8):571-578. Published online July 25, 2018; DOI: https://doi.org/10.1007/s12275-018-8267-0

349 View
0 Download
4 Crossref

Abstract PDF

Bafilomycins produced by Kitasatospora cheerisanensis KCTC- 2395 belong to the 16-membered macrolactone family plecomacrolide antibiotics. Bafilomycin B1 contains 2-amino- 3-hydroxycyclopent-2-enone (C5N), a five membered ring, which gets condensed via an amide linkage to bafilomycin polyketide. To study the biosynthetic pathway of C5N during bafilomycin biosynthesis in K. cheerisanensis KCTC2395, we attempted the functional analysis of two putative genes, encoding 5-aminolevulinic acid synthase (ALAS) and acyl- CoA ligase (ACL). The amplified putative genes for ALAS and ACL were cloned into the E. coli expression vector pET- 32a(+) plasmid, following which the soluble recombinant ALAS and ACL proteins were purified through nickel-affinity column chromatography. Through HPLC analysis of the enzyme reaction mixture, we confirmed the products of putative ALAS and ACL reaction as 5-aminolevulinic acid (5- ALA) and 5-ALA-CoA, respectively. The optimal pH for the putative ALAS reaction was 7.5, and for putative ACL reaction was 7.0, as confirmed by the colorimetric assay. Furthermore, pyridoxal 5􍿁-phosphate (PLP) was found to be an essential cofactor in the putative ALAS reaction, and ATP was a cofactor for the putative ACL catalysis. Finally, we also confirmed that the simultaneous treatment of putative ACL and putative ALAS enzymes resulted in the production of C5N compound from 5-ALA.

Citations

Citations to this article as recorded by

The Secondary Metabolites from Genus Kitasatospora: A Promising Source for Drug Discovery
Yuanjuan Wei, Guiyang Wang, Yan Li, Maoluo Gan
Chemistry & Biodiversity.2024;[Epub] CrossRef
Elucidation of the Late Steps during Hexacosalactone A Biosynthesis in Streptomyces samsunensis OUCT16-12
He Duan, Fang Wang, Chuchu Zhang, Yujing Dong, Huayue Li, Fei Xiao, Wenli Li, Haruyuki Atomi
Applied and Environmental Microbiology.2023;[Epub] CrossRef
A Secondary Metabolic Enzyme Functioned as an Evolutionary Seed of a Primary Metabolic Enzyme
Jun Kawaguchi, Hikaru Mori, Noritaka Iwai, Masaaki Wachi, Miriam Barlow
Molecular Biology and Evolution.2022;[Epub] CrossRef
Biosynthesis of Methoxymalonyl-acyl Carrier Protein (ACP) as an Extender Unit for Bafilomycin Polyketide in Streptomyces griseus DSM 2608
Nguyen Phan Kieu Hanh, Jae Yoon Hwang, Doo Hyun Nam
Biotechnology and Bioprocess Engineering.2018; 23(6): 693. CrossRef

Antifungal activity of 3-acetylbenzamide produced by actinomycete WA23-4-4 from the intestinal tract of Periplaneta americana: Xia Fang , Juan Shen , Jie Wang , Zhi-li Chen , Pei-bin lin , Zhi-yu Chen , Lin-yan Liu , Huan-xiong Zeng , Xiao-bao Jin; J. Microbiol. 2018;56(7):516-523. Published online June 28, 2018; DOI: https://doi.org/10.1007/s12275-018-7510-z

400 View
0 Download
11 Crossref

Abstract PDF

Actinomycetes are well-known for producing numerous bioactive secondary metabolites. In this study, primary screening by antifungal activity assay found one actinomycete strain WA23-4-4 isolated from the intestinal tract of Periplaneta americana that exhibited broad spectrum antifungal activity. 16S rDNA gene analysis of strain WA23-4-4 revealed close similarity to Streptomyces nogalater (AB045886) with 86.6% sequence similarity. Strain WA23-4-4 was considered as a novel Streptomyces and the 16s rDNA sequence has been submitted to GenBank (accession no. KX291006). The maximum antifungal activity of WA23-4-4 was achieved when culture conditions were optimized to pH 8.0, with 12% inoculum concentration and 210 ml ISP2 medium, which remained stable between the 5th and the 9th day. 3-Acetyl benzoyl amide was isolated by ethyl acetate extraction of WA23- 4-4 fermentation broth, and its molecular formula was determined as C9H9NO2 based on MS, IR, 1H, and 13C NMR analyses. The compound showed significant antifungal activity against Candida albicans ATCC 10231 (MIC: 31.25 μg/ml) and Aspergillus niger ATCC 16404 (MIC: 31.25 μg/ml). However, the compound had higher MIC values against Trichophyton rubrum ATCC 60836 (MIC: 500 μg/ml) and Aspergillus fumigatus ATCC 96918 (MIC: 1,000 μg/ml). SEM analysis showed damage to the cell membrane of Candida albicans ATCC 10231 and to the mycelium of Aspergillus niger ATCC 16404 after being treatment with 3-acetyl benzoyl amide. In conclusion, this is the first time that 3-acetyl benzoyl amide has been identified from an actinomycete and this compound exhibited antifungal activity against Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404.

Citations

Citations to this article as recorded by

Comparative metabolomics reveals streptophenazines with anti-methicillin-resistant Staphylococcus aureus activity derived from Streptomyces albovinaceus strain WA10-1-8 isolated from Periplaneta americana
Liuchong Zhu, Dan Huang, Jinli Tan, Jiaxuan Huang, Ruyu Zhang, Jingyang Liao, Jie Wang, Xiaobao Jin
BMC Microbiology.2025;[Epub] CrossRef
State-of-the-art review of edible insect: from bioactives, pretreatment to enrichment
Zong-Qi Zhang, Si-Cong Chen, Jin-Hua Xiao, Da-Wei Huang
Food Bioscience.2024; 59: 103879. CrossRef
Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana
Yan Ma, Ping Guo, Xueqin Chen, Minhua Xu, Wenbin Liu, Xiaobao Jin
BMC Microbiology.2023;[Epub] CrossRef
Stereochemical Control of Secondary Benzamide‐based BODIPY Emitters
Sara M. A. Waly, Andrew C. Benniston, Joshua K. G. Karlsson, Casey M. Dixon, Corinne Wills, Paul G. Waddell, Anthony Harriman
Chemistry – A European Journal.2023;[Epub] CrossRef
Antifungal activity of compounds from Gordonia sp. WA8-44 isolated from the gut of Periplaneta americana and molecular docking studies
Wenbin Liu, Ertong Li, Lingyan Liu, Fangyuan Tian, Xiongming Luo, Yanqu Cai, Jie Wang, Xiaobao Jin
Heliyon.2023; 9(7): e17777. CrossRef
A minireview of the medicinal and edible insects from the traditional Chinese medicine (TCM)
Enming Zhang, Xin Ji, Fang Ouyang, Yang Lei, Shun Deng, Haibo Rong, Xuangen Deng, Hai Shen
Frontiers in Pharmacology.2023;[Epub] CrossRef
Insects as sources of food and bioproducts: a review from Colombia
María Susana Pérez-Grisales, Sandra I. Uribe Soto
The Journal of Basic and Applied Zoology.2022;[Epub] CrossRef
Bacterial bioactive metabolites as therapeutic agents: From production to action
K.B. Arun, Raveendran Sindhu, Deepthy Alex, Parameswaran Binod, Arivalagan Pughazhendi, Toms C. Joseph, Ashok Pandey, Mohammed Kuddus, Santhosh Pillai, Shibitha Emmanual, Mukesh Kumar Awasthi, Aravind Madhavan
Sustainable Chemistry and Pharmacy.2022; 27: 100650. CrossRef
A cytotoxic triterpenoid from a Periplaneta americana-derived, Gordonia hongkongensis WA12-1-1
Jie Wang, Mengying He, Huanxiong Zeng, Wenbin Liu, Xiongming Luo, Yan Ma, Zhiyu Chen, Xiaobao Jin
FEMS Microbiology Letters.2022;[Epub] CrossRef
Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana
Yan Ma, Minhua Xu, Hancong Liu, Tiantian Yu, Ping Guo, Wenbin Liu, Xiaobao Jin
AMB Express.2021;[Epub] CrossRef
Bioactive antifungal metabolites produced by Streptomyces amritsarensis V31 help to control diverse phytopathogenic fungi
Mohammad Shahid, Bansh Narayan Singh, Shaloo Verma, Prassan Choudhary, Sudipta Das, Hillol Chakdar, Kumar Murugan, Sanjay Kumar Goswami, Anil Kumar Saxena
Brazilian Journal of Microbiology.2021; 52(4): 1687. CrossRef

The synthetic human beta-defensin-3 C15 peptide exhibits antimicrobial activity against Streptococcus mutans, both alone and in combination with dental disinfectants: Ki Bum Ahn , A Reum Kim , Kee-Yeon Kum , Cheol-Heui Yun , Seung Hyun Han; J. Microbiol. 2017;55(10):830-836. Published online September 28, 2017; DOI: https://doi.org/10.1007/s12275-017-7362-y

387 View
0 Download
29 Crossref

Abstract PDF

Streptococcus mutans is a major etiologic agent of human dental caries that forms biofilms on hard tissues in the human oral cavity, such as tooth and dentinal surfaces. Human β-defensin-3 (HBD3) is a 45-amino-acid natural antimicrobial peptide that has broad spectrum antimicrobial activity against bacteria and fungi. A synthetic peptide consisting of the C-terminal 15 amino acids of HBD3 (HBD3-C15) was recently shown to be sufficient for its antimicrobial activity. Thus, clinical applications of this peptide have garnered attention. In this study, we investigated whether HBD3-C15 inhibits the growth of the representative cariogenic pathogen Streptococcus mutans and its biofilm formation. HBD3-C15 inhibited bacterial growth, exhibited bactericidal activity, and attenuated bacterial biofilm formation in a dose-dependent manner. HBD3-C15 potentiated the bactericidal and anti-biofilm activity of calcium hydroxide (CH) and chlorhexidine digluconate (CHX), which are representative disinfectants used in dental clinics, against S. mutans. Moreover, HBD3-C15 showed antimicrobial activity by inhibiting biofilm formation by S. mutans and other dentinophilic bacteria such as Enterococcus faecalis and Streptococcus gordonii, which are associated with dental caries and endodontic infection, on human dentin slices. These effects were observed for HBD3-C15 alone and for HBD3-C15 in combination with CH or CHX. Therefore, we suggest that HBD3-C15 is a potential alternative or additive disinfectant that can be used for the treatment of oral infectious diseases, including dental caries and endodontic infections.

Citations

Citations to this article as recorded by

The Role and Progress of Antimicrobial Peptides in Managing Oral Biofilms: A Narrative Review
Deji Ciren, Xiaoyue Xia, Yuemeng Zhu, Sumin Hui, Lihua Hong
International Dental Journal.2026; 76(1): 104022. CrossRef
Comparison of salivary statherin and beta-defensin-2 levels, oral health behaviors, and demographic factors in children with and without early childhood caries
Maryam Koopaie, Faezeh Khajehreza Shahri, Roshanak Montazeri, Sajad Kolahdooz, Majid Mardani Shahri, Elham Moshkbouy
BMC Oral Health.2025;[Epub] CrossRef
Human β-defensins and their synthetic analogs: Natural defenders and prospective new drugs of oral health
Mumian Chen, Zihe Hu, Jue Shi, Zhijian Xie
Life Sciences.2024; 346: 122591. CrossRef
Addressing Antimicrobial Properties in Guided Tissue/Bone Regeneration Membrane: Enhancing Effectiveness in Periodontitis Treatment
Sara Takallu, Esmaeil Mirzaei, Abbas Zakeri Bazmandeh, Hamid Reza Ghaderi Jafarbeigloo, Hooman Khorshidi
ACS Infectious Diseases.2024; 10(3): 779. CrossRef
Novel Nano Drug‐Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI
Jibin Wu, Jianling Yan, Sijia Xu, Xuan Zou, Yinghua Xu, Xiaobao Jin, Xuemei Lu, Shuiqing Gui
Advanced Healthcare Materials.2024;[Epub] CrossRef
Harnessing antimicrobial peptides in endodontics
Xinzi Kong, Vijetha Vishwanath, Prasanna Neelakantan, Zhou Ye
International Endodontic Journal.2024; 57(7): 815. CrossRef
A Robust Strategy Against Multi-Resistant Pathogens in Oral Health: Harnessing the Potency of Antimicrobial Peptides in Nanofiber-Mediated Therapies
Karthikeyan Kandaswamy, Raghunandhakumar Subramanian, Jayant Giri, Ajay Guru, Jesu Arockiaraj
International Journal of Peptide Research and Therapeutics.2024;[Epub] CrossRef
A Novel Beta-Defensin Isoform from Malabar Trevally, Carangoides malabaricus (Bloch & Schneider, 1801), an Arsenal Against Fish Bacterial Pathogens: Molecular Characterization, Recombinant Production, and Mechanism of Action
Athira P. P., Anju M. V., Anooja V. V., Archana K., Neelima S., Muhammed Musthafa S., I. S. Bright Singh, Rosamma Philip
Marine Biotechnology.2024; 26(4): 696. CrossRef
Antimicrobial Peptides Targeting Oral Pathogens: Applicability as an Oral Disease Treatment and Dental Material
Sehyeok Im, Jun Hyuck Lee, Youn-Soo Shim
Journal of Dental Hygiene Science.2024; 24(4): 231. CrossRef
Antimicrobial and Antibiofilm Activity of Synthetic Peptide [W7]KR12-KAEK Against Enterococcus faecalis Strains
Mayron Alves Vasconcelos, Bruno Rocha da Silva, Alexandre Lopes Andrade, Aryane de Azevedo Pinheiro, Francisco Flávio Vasconcelos Evaristo, Francisco Vassiliepe Sousa Arruda, Esteban Nicolás Lorenzón, Eduardo Maffud Cilli, Edson Holanda Teixeira
Current Microbiology.2023;[Epub] CrossRef
Peptide Designs for Use in Caries Management: A Systematic Review
Olivia Lili Zhang, John Yun Niu, Ollie Yiru Yu, May Lei Mei, Nicholas Stephen Jakubovics, Chun Hung Chu
International Journal of Molecular Sciences.2023; 24(4): 4247. CrossRef
Growing Global Research Interest in Antimicrobial Peptides for Caries Management: A Bibliometric Analysis
Olivia Lili Zhang, John Yun Niu, Iris Xiaoxue Yin, Ollie Yiru Yu, May Lei Mei, Chun Hung Chu
Journal of Functional Biomaterials.2022; 13(4): 210. CrossRef
Important Roles and Potential Uses of Natural and Synthetic Antimicrobial Peptides (AMPs) in Oral Diseases: Cavity, Periodontal Disease, and Thrush
Albert Donald Luong, Alyah Buzid, John H. T. Luong
Journal of Functional Biomaterials.2022; 13(4): 175. CrossRef
Development and evaluation of a chewing gum containing antimicrobial peptide GH12 for caries prevention
Xuelian Jiang, Yufei Wang, Xinwei Li, Zening Feng, Yuhao Zeng, Sili Han, Nobuhiro Takahashi, Linglin Zhang
European Journal of Oral Sciences.2022;[Epub] CrossRef
Effect of Bacteriocin-Like Inhibitory Substance (BLIS) from Enterococcus faecium DB1 on Cariogenic Streptococcus mutans Biofilm Formation
Ni-Na Kim, Bong Sun Kim, Han Bin Lee, Sunghyun An, Donghan Kim, Seok-Seong Kang
Food Science of Animal Resources.2022; 42(6): 1020. CrossRef
In Vitro Pharmacodynamics and Bactericidal Mechanism of Fungal Defensin-Derived Peptides NZX and P2 against Streptococcus agalactiae
Yankang Wu, Na Yang, Ruoyu Mao, Ya Hao, Da Teng, Jianhua Wang
Microorganisms.2022; 10(5): 881. CrossRef
Characterization of Histopathology and Microbiota in Contemporary Regenerative Endodontic Procedures: Still Coming up Short
Yeon-Jee Yoo, Hiran Perinpanayagam, Youngnim Choi, Yu Gu, Seok-Woo Chang, Seung-Ho Baek, Qiang Zhu, Ashraf F. Fouad, Kee-Yeon Kum
Journal of Endodontics.2021; 47(8): 1285. CrossRef
The multifaceted roles of antimicrobial peptides in oral diseases
John Yun Niu, Iris Xiaoxue Yin, May Lei Mei, William Ka Kei Wu, Quan‐Li Li, Chun Hung Chu
Molecular Oral Microbiology.2021; 36(3): 159. CrossRef
Antimicrobial peptides: mechanism of action, activity and clinical potential
Qi-Yu Zhang, Zhi-Bin Yan, Yue-Ming Meng, Xiang-Yu Hong, Gang Shao, Jun-Jie Ma, Xu-Rui Cheng, Jun Liu, Jian Kang, Cai-Yun Fu
Military Medical Research.2021;[Epub] CrossRef
Antimicrobial peptides for the prevention and treatment of dental caries: A concise review
John Yun Niu, Iris Xiaoxue Yin, William Ka Kei Wu, Quan-Li Li, May Lei Mei, Chun Hung Chu
Archives of Oral Biology.2021; 122: 105022. CrossRef
Suppressive effects of gecko cathelicidin on biofilm formation and cariogenic virulence factors of Streptococcus mutans
Shasha Cai, Kai Meng, Peng Liu, Xianting Cao, Guannan Wang
Archives of Oral Biology.2021; 129: 105205. CrossRef
A Chimeric Cationic Peptide Composed of Human β-Defensin 3 and Human β-Defensin 4 Exhibits Improved Antibacterial Activity and Salt Resistance
Wenjing Yu, Nianzhi Ning, Ying Xue, Yanyu Huang, Feng Guo, Tao Li, Boning Yang, Deyan Luo, Yakun Sun, Zhan Li, Jianxin Wang, Zhili He, Shiwei Cheng, Xingxiao Zhang, Hui Wang
Frontiers in Microbiology.2021;[Epub] CrossRef
Oral antimicrobial peptides and new therapeutic strategies for plaque-mediated diseases
Nafiseh Izadi, Masoud Keikha, Kiarash Ghazvini, Mohsen Karbalaei
Gene Reports.2020; 21: 100811. CrossRef
Antimicrobial peptide GH12 as root canal irrigant inhibits biofilm and virulence of Enterococcus faecalis
Y. Li, Y. Wang, X. Chen, W. Jiang, X. Jiang, Y. Zeng, X. Li, Z. Feng, J. Luo, L. Zhang
International Endodontic Journal.2020; 53(7): 948. CrossRef
Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans
Kaixin Xiong, Xuan Chen, Hantao Hu, Huihui Hou, Peng Gao, Ling Zou, György Schneider
BioMed Research International.2020;[Epub] CrossRef
In Vitro Evaluation of the Antibacterial Properties of Tea Tree Oil on Planktonic and Biofilm-Forming Streptococcus mutans
Yu-Meng Song, Hong-Yan Zhou, Ye Wu, Jue Wang, Qian Liu, Yu-Feng Mei
AAPS PharmSciTech.2020;[Epub] CrossRef
Endodontic biofilms: contemporary and future treatment options
Yeon-Jee Yoo, Hiran Perinpanayagam, Soram Oh, A-Reum Kim, Seung-Hyun Han, Kee-Yeon Kum
Restorative Dentistry & Endodontics.2019;[Epub] CrossRef
Antimicrobial peptide GH12 targetsStreptococcus mutansto arrest caries development in rats
Yufei Wang, Yuhao Zeng, Yuanjing Wang, Haoran Li, Sihan Yu, Wentao Jiang, Yingxue Li, Linglin Zhang
Journal of Oral Microbiology.2019; 11(1): 1549921. CrossRef
A New Combination with D-Cateslytin to Eradicate Root Canal Pathogens
Claire Ehlinger, Pauline Dartevelle, Abdurraouf Zaet, Yoshihito Kurashige, Youssef Haïkel, Marie-Hélène Metz-Boutigue, Céline Marban
International Journal of Peptide Research and Therapeutics.2019; 25(4): 1679. CrossRef

Minimal amino acids in the I/LWEQ domain required for anterior/posterior localization in Dictyostelium: Hyeseon Kim , Dong-Yeop Shin , Taeck Joong Jeon; J. Microbiol. 2017;55(5):366-372. Published online January 26, 2017; DOI: https://doi.org/10.1007/s12275-017-6550-0

213 View
0 Download
1 Scopus

Abstract PDF: Establishment of cell polarity is mediated by a series of signal-ing molecules that are asymmetrically activated or localized in the cell upon extracellular stimulation. To understand the mechanism that mediates anterior/posterior asymmetric localization of RapGAP3 during migration, we determined the minimally required amino acids in the I/LWEQ domain that cause posterior localization and found that the minimal region of the F-actin binding domain for posterior localiza-tion could, with some additional deletion at the C-terminal, localize to the anterior. Analysis of the localization and trans-location kinetics to the cell cortex of the truncated proteins suggests that the required regions for anterior/posterior lo-calization might have a preferential binding affinity to pre- existing F-actins at the rear and lateral sides of the cell or newly formed F-actins at the front of the cell, leading to dis-tinct differential sites of the cell.

The antibacterial activity of E. coli bacteriophage lysin lysep3 is enhanced by fusing the Bacillus amyloliquefaciens bacteriophage endolysin binding domain D8 to the C-terminal region: Shuang Wang , Jingmin Gu , Meng Lv , Zhimin Guo , Guangmou Yan , Ling Yu , Chongtao Du , Xin Feng , Wenyu Han , Changjiang Sun , Liancheng Lei; J. Microbiol. 2017;55(5):403-408. Published online January 26, 2017; DOI: https://doi.org/10.1007/s12275-017-6431-6

408 View
0 Download
35 Crossref

Abstract PDF

Bacteriophage endolysin is one of the most promising anti-biotic substitutes, but in Gram-negative bacteria, the outer membrane prevents the lysin from hydrolyzing peptidogly-cans and blocks the development of lysin applications. The prime strategy for new antibiotic substitutes is allowing lysin to access the peptidoglycan from outside of the bacteria by reformation of the lysin. In this study, the novel Escherichia coli (E. coli) phage lyase lysep3, which lacks outside-in cata-lytic ability, was fused with the N-terminal region of the Bacillus amyloliquefaciens lysin including its cell wall bind-ing domain D8 through the best manner of protein fusion based on the predicted tertiary structure of lysep3-D8 to ob-tain an engineered lysin that can lyse bacteria from the out-side. Our results showed that lysep3-D8 could lyse both Gram- negative and Gram-positive bacteria, whereas lysep3 and D8 have no impact on bacterial growth. The MIC of lysep3-D8 on E. coli CVCC1418 is 60 μg/ml; lysep3-D8 can inhibit the growth of bacteria up to 12 h at this concentration. The bac-tericidal spectrum of lysep3-D8 is broad, as it can lyse of all of 14 E. coli strains, 3 P. aeruginosa strains, 1 Acinetobacter baumannii strain, and 1 Streptococcus strain. Lysep3-D8 has sufficient bactericidal effects on the 14 E. coli strains tested at the concentration of 100 μg/ml. The cell wall binding do-main of the engineered lysin can destroy the integrity of the outer membrane of bacteria, thus allowing the catalytic do-main to reach its target, peptidoglycan, to lyse the bacteria. Lysep3-D8 can be used as a preservative in fodder to benefit the health of animals. The method we used here proved to be a successful exploration of the reformation of phage lysin.

Citations

Citations to this article as recorded by

Advances in the development of phage-mediated cyanobacterial cell lysis
Haojie Jin, Wanzhao Ge, Mengzhe Li, Yan Wang, Yanjing Jiang, Jiaqi Zhang, Yike Jing, Yigang Tong, Yujie Fu
Critical Reviews in Biotechnology.2025; 45(4): 1002. CrossRef
Phage Endolysins as Promising and Effective Candidates for Use Against Uropathogenic Escherichia coli
Wojciech Wesołowski, Aleksandra Łukasiak, Sylwia Bloch, Kaja Kuligowska, Julia Neumann, Natalia Lewandowska, Emilia Węglińska, Grzegorz Węgrzyn, Bożena Nejman-Faleńczyk
Viruses.2025; 17(4): 560. CrossRef
Endolysins and membrane-active peptides: innovative engineering strategies against gram-negative bacteria
Monika Wojciechowska
Frontiers in Microbiology.2025;[Epub] CrossRef
Engineered Phages and Engineered and Recombinant Endolysins Against Carbapenem‐Resistant Gram‐Negative Bacteria: A Focused Review on Novel Antibacterial Strategies
Majid Taati Moghadam, Shaghayegh Shahkolahi, Israa A. Hashim, Shamsieh Asgharihajimahalleh, Ghazale Khodadadi, Fatemeh Moghbeli, Samira Sabzi, Shahla Shahbazi
Journal of Basic Microbiology.2025;[Epub] CrossRef
Characterization of LysBM1, a novel high-penetrating phage lysin targeting enterohemorrhagic Escherichia coli
Haitao Liu, Lichang Sun, Quan Sun, Shiqiang Zhang, Ran Wang
Frontiers in Veterinary Science.2025;[Epub] CrossRef
Engineering strategies and challenges of endolysin as an antibacterial agent against Gram‐negative bacteria
Tianyu Zheng, Can Zhang
Microbial Biotechnology.2024;[Epub] CrossRef
Role of hypothetical protein PA1-LRP in antibacterial activity of endolysin from a new Pantoea phage PA1
Ye Tian, Xinyan Xu, Munazza Ijaz, Ying Shen, Muhammad Shafiq Shahid, Temoor Ahmed, Hayssam M. Ali, Chengqi Yan, Chunyan Gu, Jianfei Lu, Yanli Wang, Gabrijel Ondrasek, Bin Li
Frontiers in Microbiology.2024;[Epub] CrossRef
New-Generation Antibacterial Agent—Cellulose-Binding Thermostable TP84_Endolysin
Małgorzata Ponikowska, Joanna Żebrowska, Piotr M. Skowron
International Journal of Molecular Sciences.2024; 25(23): 13111. CrossRef
Characterization of Pseudomonas aeruginosa bacteriophages and control hemorrhagic pneumonia on a mice model
Yanjie Zhang, Ruiqing Wang, Qingxia Hu, Ni Lv, Likun Zhang, Zengqi Yang, Yefei Zhou, Xinglong Wang
Frontiers in Microbiology.2024;[Epub] CrossRef
Biological and genomic characterization of a polyvalent phage PSH-1 against multidrug-resistant Salmonella Enteritidis
Shuai-Hua Li, Rui-Yun Wang, Jun-Kai Zhang, Kai-Fang Yi, Jian-Hua Liu, Hua Wu, Li Yuan, Ya-Jun Zhai, Gong-Zheng Hu
BMC Microbiology.2024;[Epub] CrossRef
Beyond antibiotics: phage-encoded lysins against Gram-negative pathogens
Sanket Shah, Ritam Das, Bhakti Chavan, Urmi Bajpai, Sarmad Hanif, Syed Ahmed
Frontiers in Microbiology.2023;[Epub] CrossRef
The Broad-Spectrum Endolysin LySP2 Improves Chick Survival after Salmonella Pullorum Infection
Hewen Deng, Mengjiao Li, Qiuyang Zhang, Chencheng Gao, Zhanyun Song, Chunhua Chen, Zhuo Wang, Xin Feng
Viruses.2023; 15(4): 836. CrossRef
Development of a Magnetic Bead-Based Method for Specific Detection of Enterococcus faecalis Using C-Terminal Domain of ECP3 Phage Endolysin
Yoon-Jung Choi, Shukho Kim, Jungmin Kim
Journal of Microbiology and Biotechnology.2023; 33(7): 964. CrossRef
Identification of Three Campylobacter Lysins and Enhancement of Their Anti-Escherichia coli Efficacy Using Colicin-Based Translocation and Receptor-Binding Domain Fusion
Peiqi Liu, Xinying Dong, Xuewei Cao, Qianmei Xie, Xiuqin Huang, Jinfei Jiang, Huilin Dai, Zheng Tang, Yizhen Lin, Saixiang Feng, Kaijian Luo, Jasna Kovac
Microbiology Spectrum.2023;[Epub] CrossRef
Isolation of Klebsiella pneumoniae Phage vB_KpnS_MK54 and Pathological Assessment of Endolysin in the Treatment of Pneumonia Mice Model
Biao Lu, Xueping Yao, Guangli Han, Zidan Luo, Jieru Zhang, Kang Yong, Yin Wang, Yan Luo, Zexiao Yang, Meishen Ren, Suizhong Cao
Frontiers in Microbiology.2022;[Epub] CrossRef
How Good are Bacteriophages as an Alternative Therapy to Mitigate Biofilms of Nosocomial Infections
Aditi Singh, Sudhakar Padmesh, Manish Dwivedi, Irena Kostova
Infection and Drug Resistance.2022; Volume 15: 503. CrossRef
Complete genome sequencing of a Tequintavirus bacteriophage with a broad host range against Salmonella Abortus equi isolates from donkeys
Wenhua Liu, Letian Han, Peng Song, Huzhi Sun, Can Zhang, Ling Zou, Jiaqi Cui, Qiang Pan, Huiying Ren
Frontiers in Microbiology.2022;[Epub] CrossRef
Characterization of Salmonella endolysin XFII produced by recombinant Escherichia coli and its application combined with chitosan in lysing Gram-negative bacteria
Shuhang Zhang, Yan Chang, Qing Zhang, Yingbo Yuan, Qingsheng Qi, Xuemei Lu
Microbial Cell Factories.2022;[Epub] CrossRef
The endolysin of the Acinetobacter baumannii phage vB_AbaP_D2 shows broad antibacterial activity
Yuyu Yuan, Xiaoyu Li, Lili Wang, Gen Li, Cong Cong, Ruihua Li, Huijing Cui, Bilal Murtaza, Yongping Xu
Microbial Biotechnology.2021; 14(2): 403. CrossRef
Lysins breaking down the walls of Gram-negative bacteria, no longer a no-go
Diana Gutiérrez, Yves Briers
Current Opinion in Biotechnology.2021; 68: 15. CrossRef
The Advantages and Challenges of Using Endolysins in a Clinical Setting
Ellen Murray, Lorraine A. Draper, R. Paul Ross, Colin Hill
Viruses.2021; 13(4): 680. CrossRef
The strategy of biopreservation of meat product against MRSA using lytic domain of lysin from Staphylococcus aureus bacteriophage
Jiai Yan, Ruijin Yang, Shuhuai Yu, Wei Zhao
Food Bioscience.2021; 41: 100967. CrossRef
Phage vB_PaeS-PAJD-1 Rescues Murine Mastitis Infected With Multidrug-Resistant Pseudomonas aeruginosa
Zhaofei Wang, Yibing Xue, Ya Gao, Mengting Guo, Yuanping Liu, Xinwei Zou, Yuqiang Cheng, Jingjiao Ma, Hengan Wang, Jianhe Sun, Yaxian Yan
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Opportunities for broadening the application of cell wall lytic enzymes
Amala Bhagwat, Monica Mixon, Cynthia H. Collins, Jonathan S. Dordick
Applied Microbiology and Biotechnology.2020; 104(21): 9019. CrossRef
Gram-Negative Bacterial Lysins
Chandrabali Ghose, Chad W. Euler
Antibiotics.2020; 9(2): 74. CrossRef
Bacteriophage-derived endolysins to target gram-negative bacteria
Wing Ching Bianca Lai, Xi Chen, Marco Kai Yuen Ho, Jiang Xia, Sharon Shui Yee Leung
International Journal of Pharmaceutics.2020; 589: 119833. CrossRef
Bacteriophages and Lysins as Possible Alternatives to Treat Antibiotic-Resistant Urinary Tract Infections
Trinidad de Miguel, José Luis R. Rama, Carmen Sieiro, Sandra Sánchez, Tomas G. Villa
Antibiotics.2020; 9(8): 466. CrossRef
Managing urinary tract infections through phage therapy: a novel approach
Shikha Malik, Parveen Kaur Sidhu, J.S. Rana, Kiran Nehra
Folia Microbiologica.2020; 65(2): 217. CrossRef
Endolysins as emerging alternative therapeutic agents to counter drug-resistant infections
Vijay Singh Gondil, Kusum Harjai, Sanjay Chhibber
International Journal of Antimicrobial Agents.2020; 55(2): 105844. CrossRef
External lysis of Escherichia coli by a bacteriophage endolysin modified with hydrophobic amino acids
Guangmou Yan, Rui Yang, Kejia Fan, Hanlin Dong, Chencheng Gao, Shuang Wang, Ling Yu, Zhe Cheng, Liancheng Lei
AMB Express.2019;[Epub] CrossRef
Phage Lysins for Fighting Bacterial Respiratory Infections: A New Generation of Antimicrobials
Roberto Vázquez, Ernesto García, Pedro García
Frontiers in Immunology.2018;[Epub] CrossRef
Synthetic biology of modular endolysins
Hans Gerstmans, Bjorn Criel, Yves Briers
Biotechnology Advances.2018; 36(3): 624. CrossRef
Isolation and identification of Salmonella pullorum bacteriophage YSP2 and its use as a therapy for chicken diarrhea
Kunyuan Tie, Yuyu Yuan, Shiqing Yan, Xi Yu, Qiuyang Zhang, Huihui Xu, Yang Zhang, Jingmin Gu, Changjiang Sun, Liancheng Lei, Wenyu Han, Xin Feng
Virus Genes.2018; 54(3): 446. CrossRef
Engineering of Phage-Derived Lytic Enzymes: Improving Their Potential as Antimicrobials
Carlos São-José
Antibiotics.2018; 7(2): 29. CrossRef
Expression of a Peptidoglycan Hydrolase from Lytic Bacteriophages Atu_ph02 and Atu_ph03 Triggers Lysis of Agrobacterium tumefaciens
Hedieh Attai, Jeanette Rimbey, George P. Smith, Pamela J. B. Brown, M. Julia Pettinari
Applied and Environmental Microbiology.2017;[Epub] CrossRef

Siphonobacter intestinalis sp. nov., a bacterium isolated from the feces of Pseudorhynchus japonicus: Shin Ae Lee , Jeong Myeong Kim , Jae-Hyung Ahn , Jae-Ho Joa , Soo-Jin Kim , Mee-Kyung Sang , Jaekyeong Song , Soon-Wo Kwon , Hang-Yeon Weon; J. Microbiol. 2016;54(11):709-712. Published online October 29, 2016; DOI: https://doi.org/10.1007/s12275-016-6451-7

334 View
0 Download
1 Crossref

Abstract PDF

Strain 63MJ-2T was isolated from the feces of broad-winged katydid (Pseudorhynchus japonicus) collected in Korea. The 16S rRNA gene sequence of this strain showed the highest sequence similarity with that of Siphonobacter aquaeclarae P2T (96.1%) and had low similarities (below 86.3%) with those of other members of family ‘Flexibacteraceae’. The strain 63MJ-2T is a strictly aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium. The strain grew at 4–35°C (optimum, 25–30°C), pH of 5.0–9.0 (optimum, 6.0–7.0), and 0–2.0% (optimum, 1.0–2.0) (w/v) NaCl. The DNA G+C content of strain 63MJ-2T was 43.5 mol%. The major fatty acids were C16:1 ω5c (42.5%), iso-C17:0 3-OH (18.7%), and summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c, 18.0%). The major menaquinone was MK-7 and polar lipids were phosphatidylethanolamine, six unknown aminolipids, and five unknown lipids. Based on the evidence from our polyphasic taxonomic study, we conclude that strain 63MJ-2T should be classified as a novel species of the genus Siphonobacter, and propose the name Siphonobacter intestinalis sp. nov. The type strain is 63MJ-2T (=KACC 18663T =NBRC 111883T).

Citations

Citations to this article as recorded by

Siphonobacter curvatus sp. nov., isolated from a freshwater river
Yunho Lee, Che Ok Jeon
International Journal of Systematic and Evolutionary Microbiology .2018; 68(6): 1925. CrossRef

Mycobacterium tuberculosis gene expression at different stages of hypoxia-induced dormancy and upon resuscitation: Elisabetta Iona , Manuela Pardini , Alessandro Mustazzolu , Giovanni Piccaro , Roberto Nisini , Lanfranco Fattorini , Federico Giannoni; J. Microbiol. 2016;54(8):565-572. Published online August 2, 2016; DOI: https://doi.org/10.1007/s12275-016-6150-4

450 View
0 Download
41 Crossref

Abstract PDF

The physiology of dormant Mycobacterium tuberculosis was studied in detail by examining the gene expression of 51 genes using quantitative Reverse-Transcription Polymerase Chain Reaction. A forty-day period of dormancy in the Wayne culture model depicted four major transcription patterns. Some sigma factors and many metabolic genes were constant, whereas genes belonging to the dormancy regulon were activated on day 9. In particular, alpha-crystallin mRNA showed more than a 1,000-fold increase compared to replicating bacilli. Genes belonging to the enduring hypoxic response were up-regulated at day 16, notably, transcription factors sigma B and E. Early genes typical of log-phase bacilli, esat-6 and fbpB, were uniformly down-regulated during dormancy. Late stages of dormancy showed a drop in gene expression likely due to a lack of substrates in anaerobic respiration as demonstrated by the transcriptional activation observed following nitrates addition. Among genes involved in nitrate metabolism, narG was strongly up-regulated by nitrates addition. Dormant bacilli responded very rapidly when exposed to oxygen and fresh medium, showing a transcriptional activation of many genes, including resuscitation-promoting factors, within one hour. Our observations extend the current knowledge on dormant M. tuberculosis gene expression and its response to nutrients and to aerobic and anaerobic respiration.

Citations

Citations to this article as recorded by

Expression of Resuscitation‐Promoting Factor C Stimulates the Growth of Mycobacterium bovis BCG and Delays DevR Regulon Activation in Hypoxia
Luz Virginia Reyes-González, Olga N. Hernández de la Cruz, Mauricio Castañón-Arreola, Saeed Mohammadi
International Journal of Microbiology.2025;[Epub] CrossRef
Macrophage Differentiation and Metabolic Adaptation in Mycobacterial Infections
Anne Kathrin Lösslein, Philipp Henneke
Annual Review of Immunology .2025; 43(1): 423. CrossRef
Biochemistry of Reactivation of Dormant Mycobacteria
Margarita O. Shleeva, Galina R. Demina, Arseny S. Kaprelyants
Biochemistry (Moscow).2025; 90(S1): S193. CrossRef
Regulatory role of Mycobacterium tuberculosis MtrA on dormancy/resuscitation revealed by a novel target gene-mining strategy
Xiang Fu, Xiaoyu Wan, Aadil Ahmed Memon, Xiao-Yong Fan, Qiuhong Sun, Haifeng Chen, Yufeng Yao, Zixin Deng, Jian Ma, Wei Ma
Frontiers in Microbiology.2024;[Epub] CrossRef
Adaptation of the Mycobacterium tuberculosis transcriptome to biofilm growth
Madison A. Youngblom, Tracy M. Smith, Holly J. Murray, Caitlin S. Pepperell, Christopher M. Sassetti
PLOS Pathogens.2024; 20(4): e1012124. CrossRef
Identification of Rv1133c (MetE) as a marker of Mycobacterium tuberculosis replication and as a highly immunogenic antigen with potential immunodiagnostic power
Angelo Iacobino, Raffaela Teloni, Carmine Mancone, Francesco Facchiano, Alessandra Di Giamberardino, Cinzia Senatore, Antonio Di Virgilio, Alessio Lanni, Federico Giannoni, Roberto Nisini, Sabrina Mariotti
Frontiers in Immunology.2024;[Epub] CrossRef
Advances in understanding immune homeostasis in latent tuberculosis infection
Liangfei Niu, Hao Wang, Geyang Luo, Jing Zhou, Zhidong Hu, Bo Yan
WIREs Mechanisms of Disease.2024;[Epub] CrossRef
Microneedle patch-based enzyme-linked immunosorbent assay to quantify protein biomarkers of tuberculosis
Youngeun Kim, Mary Beth Lewis, Jihyun Hwang, Zheyu Wang, Rohit Gupta, Yuxiong Liu, Tuhina Gupta, James P. Barber, Srikanth Singamaneni, Fred Quinn, Mark R. Prausnitz
Biomedical Microdevices.2024;[Epub] CrossRef
Mycobacterium tuberculosis Adaptation in Response to Isoniazid Treatment in a Multi-Stress System That Mimics the Host Environment
Manita Yimcharoen, Sukanya Saikaew, Usanee Wattananandkul, Ponrut Phunpae, Sorasak Intorasoot, Chatchai Tayapiwatana, Bordin Butr-Indr
Antibiotics.2023; 12(5): 852. CrossRef
Eradication of Drug-Tolerant Mycobacterium tuberculosis 2022: Where We Stand
Alessio Lanni, Angelo Iacobino, Lanfranco Fattorini, Federico Giannoni
Microorganisms.2023; 11(6): 1511. CrossRef
Intranasal multivalent adenoviral-vectored vaccine protects against replicating and dormant M.tb in conventional and humanized mice
Sam Afkhami, Michael R. D’Agostino, Maryam Vaseghi-Shanjani, Madeleine Lepard, Jack X. Yang, Rocky Lai, Margaret Wa Yan Choi, Alexis Chacon, Anna Zganiacz, Kees L. M. C. Franken, Hildegund C. Ertl, Tom H. M. Ottenhoff, Mangalakumari Jeyanathan, Amy Gillgr
npj Vaccines.2023;[Epub] CrossRef
WhiB4 Is Required for the Reactivation of Persistent Infection of Mycobacterium marinum in Zebrafish
Chen Lin, Yuting Tang, Yuchen Wang, Junli Zhang, Yeyu Li, Shuqin Xu, Bin Xia, Qiran Zhai, Yao Li, Lu Zhang, Jun Liu, Amit Singh
Microbiology Spectrum.2022;[Epub] CrossRef
Mycobacterium tuberculosis whiB3 and Lipid Metabolism Genes Are Regulated by Host Induced Oxidative Stress
Omar M. Barrientos, Elizabeth Langley, Yolanda González, Carlos Cabello, Martha Torres, Silvia Guzmán-Beltrán
Microorganisms.2022; 10(9): 1821. CrossRef
Optimal Detection of Latent Mycobacterium tuberculosis Infection by Combined Heparin-Binding Hemagglutinin (HBHA) and Early Secreted Antigenic Target 6 (ESAT-6) Whole-Blood Interferon Gamma Release Assays
V. Dirix, N. Dauby, M. Hites, E. Watelet, A. Van Praet, A. Godefroid, E. Petit, M. Singh, C. Locht, F. Mascart, V. Corbière, Christine Y. Turenne
Journal of Clinical Microbiology.2022;[Epub] CrossRef
Tuberculosis: The success tale of less explored dormant Mycobacterium tuberculosis
Akanksha Verma, Antara Ghoshal, Ved Prakash Dwivedi, Ashima Bhaskar
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Mycobacterial PPE36 Modulates Host Inflammation by Promoting E3 Ligase Smurf1-Mediated MyD88 Degradation
Zhangli Peng, Yan Yue, Sidong Xiong
Frontiers in Immunology.2022;[Epub] CrossRef
Immuno-Diagnosis of Active Tuberculosis by a Combination of Cytokines/Chemokines Induced by Two Stage-Specific Mycobacterial Antigens: A Pilot Study in a Low TB Incidence Country
Violette Dirix, Philippe Collart, Anne Van Praet, Maya Hites, Nicolas Dauby, Sabine Allard, Judith Racapé, Mahavir Singh, Camille Locht, Françoise Mascart, Véronique Corbière
Frontiers in Immunology.2022;[Epub] CrossRef
The Regulation of ManLAM-Related Gene Expression in Mycobacterium tuberculosis with Different Drug Resistance Profiles Following Isoniazid Treatment
Manita Yimcharoen, Sukanya Saikaew, Usanee Wattananandkul, Ponrut Phunpae, Sorasak Intorasoot, Watchara Kasinrerk, Chatchai Tayapiwatana, Bordin Butr-Indr
Infection and Drug Resistance.2022; Volume 15: 399. CrossRef
Implications of drug-induced phenotypical resistance: Is isoniazid radicalizing M. tuberculosis?
RJH Hammond, Frank Kloprogge, O. Della Pasqua, Stephen H. Gillespie
Frontiers in Antibiotics.2022;[Epub] CrossRef
Strain-Specific Behavior of Mycobacterium tuberculosis in Interruption of Autophagy Pathway in Human Alveolar Type II Epithelial A549 Cells
Nasim Ebrahimifard, Shima Hadifar, Mansour Kargarpour Kamakoli, Ava Behrouzi, Sharareh Khanipour, Abolfazl Fateh, Seyed Davar Siadat, Farzam Vaziri
Iranian Biomedical Journal.2022; 26(4): 313. CrossRef
Mycobacterium tuberculosis Dormancy: How to Fight a Hidden Danger
Elena G. Salina, Vadim Makarov
Microorganisms.2022; 10(12): 2334. CrossRef
Applications of Transcriptomics and Proteomics for Understanding Dormancy and Resuscitation in Mycobacterium tuberculosis
Manikuntala Kundu, Joyoti Basu
Frontiers in Microbiology.2021;[Epub] CrossRef
Differential Isoniazid Response Pattern Between Active and Dormant Mycobacterium tuberculosis
M.A. Abo-Kadoum, Yongdong Dai, Mohammed Asaad, Insaf Hamdi, Jianping Xie
Microbial Drug Resistance.2021; 27(6): 768. CrossRef
Insights into the evolutionary history of the virulent factor HBHA of Mycobacterium tuberculosis
Mariana P. Lanfranconi, Ana Arabolaza, Hugo Gramajo, Héctor M. Alvarez
Archives of Microbiology.2021; 203(5): 2171. CrossRef
Aptamer-Based Diagnostic Systems for the Rapid Screening of TB at the Point-of-Care
Darius Riziki Martin, Nicole Remaliah Sibuyi, Phumuzile Dube, Adewale Oluwaseun Fadaka, Ruben Cloete, Martin Onani, Abram Madimabe Madiehe, Mervin Meyer
Diagnostics.2021; 11(8): 1352. CrossRef
Transcriptome Changes of Mycobacterium marinum in the Process of Resuscitation From Hypoxia-Induced Dormancy
Jun Jiang, Chen Lin, Junli Zhang, Yuchen Wang, Lifang Shen, Kunpeng Yang, Wenxuan Xiao, Yao Li, Lu Zhang, Jun Liu
Frontiers in Genetics.2020;[Epub] CrossRef
Immunoinformatics Approach to Engineer a Potent Poly-epitope Fusion Protein Vaccine Against Coxiella burnetii
Ehsan Rashidian, Zeinab Shakarami Gandabeh, Ali Forouharmehr, Narges Nazifi, Nemat Shams, Amin Jaydari
International Journal of Peptide Research and Therapeutics.2020; 26(4): 2191. CrossRef
Coordinate regulation of virulence and metabolic genes by the transcription factor HbhR in Mycobacterium marinum
Dominique Raze, Jérôme Segers, Céline Mille, Stéphanie Slupek, Sophie Lecher, Loïc Coutte, Rudy Antoine, Lucie Ducrocq, Carine Rouanet, Ben J. Appelmelk, Camille Locht
Molecular Microbiology.2020; 113(1): 52. CrossRef
Small RNA Mcr11 requires the transcription factor AbmR for stable expression and regulates genes involved in the central metabolism of Mycobacterium tuberculosis
Roxie C. Girardin, Kathleen A. McDonough
Molecular Microbiology.2020; 113(2): 504. CrossRef
Resuscitation of Dormant “Non-culturable” Mycobacterium tuberculosis Is Characterized by Immediate Transcriptional Burst
Elena G. Salina, Artem S. Grigorov, Oksana S. Bychenko, Yulia V. Skvortsova, Ilgar Z. Mamedov, Tatyana L. Azhikina, Arseny S. Kaprelyants
Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef
Defining the Transcriptional and Post-transcriptional Landscapes of Mycobacterium smegmatis in Aerobic Growth and Hypoxia
M. Carla Martini, Ying Zhou, Huaming Sun, Scarlet S. Shell
Frontiers in Microbiology.2019;[Epub] CrossRef
Immunoscreening of the M. tuberculosis F15/LAM4/KZN secretome library against TB patients′ sera identifies unique active- and latent-TB specific biomarkers
Thamsanqa E. Chiliza, Manormoney Pillay, Kogieleum Naidoo, Balakrishna Pillay
Tuberculosis.2019; 115: 161. CrossRef
Modeling of Mycobacterium tuberculosis dormancy in bacterial cultures
Yana R. Batyrshina, Yakov Sh Schwartz
Tuberculosis.2019; 117: 7. CrossRef
The Combination Rifampin-Nitazoxanide, but Not Rifampin-Isoniazid-Pyrazinamide-Ethambutol, Kills Dormant Mycobacterium tuberculosis in Hypoxia at Neutral pH
Angelo Iacobino, Federico Giannoni, Manuela Pardini, Giovanni Piccaro, Lanfranco Fattorini
Antimicrobial Agents and Chemotherapy.2019;[Epub] CrossRef
Bacteriophage gene products as potential antimicrobials against tuberculosis
Maria Puiu, Christina Julius
Biochemical Society Transactions.2019; 47(3): 847. CrossRef
The role of epigenetics, bacterial and host factors in progression of Mycobacterium tuberculosis infection
Musa Marimani, Aijaz Ahmad, Adriano Duse
Tuberculosis.2018; 113: 200. CrossRef
Host-pathogen redox dynamics modulate Mycobacterium tuberculosis pathogenesis
Hayden T Pacl, Vineel P Reddy, Vikram Saini, Krishna C Chinta, Adrie J C Steyn
Pathogens and Disease.2018;[Epub] CrossRef
Heparin-Binding Hemagglutinin Adhesin (HBHA) Is Involved in Intracytosolic Lipid Inclusions Formation in Mycobacteria
Dominique Raze, Claudie Verwaerde, Gaspard Deloison, Elisabeth Werkmeister, Baptiste Coupin, Marc Loyens, Priscille Brodin, Carine Rouanet, Camille Locht
Frontiers in Microbiology.2018;[Epub] CrossRef
Opening Pandora’s Box: Mechanisms of Mycobacterium tuberculosis Resuscitation
Ashley V. Veatch, Deepak Kaushal
Trends in Microbiology.2018; 26(2): 145. CrossRef
AbmR (Rv1265) is a novel transcription factor of Mycobacterium tuberculosis that regulates host cell association and expression of the non‐coding small RNA Mcr11
Roxie C. Girardin, Guangchun Bai, Jie He, Haixin Sui, Kathleen A. McDonough
Molecular Microbiology.2018; 110(5): 811. CrossRef
Horizontal acquisition of a hypoxia-responsive molybdenum cofactor biosynthesis pathway contributed to Mycobacterium tuberculosis pathoadaptation
Florence Levillain, Yannick Poquet, Ludovic Mallet, Serge Mazères, Michael Marceau, Roland Brosch, Franz-Christoph Bange, Philip Supply, Axel Magalon, Olivier Neyrolles, Marcel A. Behr
PLOS Pathogens.2017; 13(11): e1006752. CrossRef

Characteristics of the community-genotype sequence type 72 methicillin-resistant Staphylococcus aureus isolates that underlie their persistence in hospitals: Eun-Jeong Joo , Ji-Young Choi , Doo Ryeon Chung , Jae-Hoon Song , Kwan Soo Ko; J. Microbiol. 2016;54(6):445-450. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6157-x

339 View
0 Download
17 Crossref

Abstract PDF

Panton-Valentine leukocidin-negative methicillin-resistant Staphylococcus aureus (MRSA) clone ST72, known as a major community-associated MRSA in Korea, has emerged as an important pathogen in hospitals. To understand bacterial properties that underlie transformation of this clone into a nosocomial pathogen, we compared characteristics of the community-genotype ST72 MRSA isolates with those of ST5 and ST239 MRSA, which have been predominant nosocomial MRSA clones in Korea. Several genes associated with adhesion and virulence were absent or rarely found in ST72 isolates. Many ST72 isolates (70.1%) belonged to agr group I, but the agr group of other ST72 isolates could not be determined. As indicated by δ-hemolysin production, ST72 isolates expressed fully functional agr, whereas agr dysfunction was observed in ST5 and ST239 isolates. In the biofilm formation assay, no upregulation of biofilm-forming activity of ST72 MRSA was detected. However, ST72 isolates demonstrated persistence under hypotonic and desiccating conditions (survival rates 72.3% and 33.9%, respectively), which was similar to characteristics of ST5 or ST239 isolates. ST72- MRSA isolates showed low virulence, but properties of their functional agr system could facilitate their spread in hospitals. In conclusion, tolerance to stressful environments, e.g., hypotonic and dry conditions, may also contribute to survival of the community-associated MRSA clones in healthcare facilities.

Citations

Citations to this article as recorded by

Multiple genome analysis of Staphylococcus aureus from wild ungulates renders new insights into the links between genetic diversity and biofilm formation
Sara Teixeira, Beatriz Ramos, Mónica V Cunha
Journal of Applied Microbiology.2025;[Epub] CrossRef
Dynamics of the MRSA Population in a Chilean Hospital: a Phylogenomic Analysis (2000–2016)
José R. W. Martínez, Paul J. Planet, Maria Spencer-Sandino, Lina Rivas, Lorena Díaz, Ahmed M. Moustafa, Ana Quesille-Villalobos, Roberto Riquelme-Neira, Manuel Alcalde-Rico, Blake Hanson, Lina P. Carvajal, Sandra Rincón, Jinnethe Reyes, Marusella Lam, Jua
Microbiology Spectrum.2023;[Epub] CrossRef
Comparing the Phylogenetic Distribution of Multilocus Sequence Typing, Staphylococcal Protein A, and Staphylococcal Cassette Chromosome Mec Types in Methicillin-Resistant Staphylococcus Aureus (MRSA) in Korea from 1994 to 2020
You-Jin Hwang
Antibiotics.2023; 12(9): 1397. CrossRef
Performance Evaluation of the IR Biotyper® System for Clinical Microbiology: Application for Detection of Staphylococcus aureus Sequence Type 8 Strains
Jun Sung Hong, Dokyun Kim, Seok Hoon Jeong
Antibiotics.2022; 11(7): 909. CrossRef
Comparative Genomic Analysis Provides Insights into the Evolution and Genetic Diversity of Community-Genotype Sequence Type 72 Staphylococcus aureus Isolates
Wangxiao Zhou, Ye Jin, Yanzi Zhou, Yuan Wang, Luying Xiong, Qixia Luo, Yonghong Xiao, Suleyman Yildirim
mSystems.2021;[Epub] CrossRef
C-reactive protein predicts persistent bacteremia caused by community-acquired methicillin-resistant Staphylococcus aureus strain
Hyemin Chung, Eunsil Kim, Eunmi Yang, Yun Woo Lee, Joung Ha Park, Seongman Bae, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim
European Journal of Clinical Microbiology & Infectious Diseases.2021; 40(12): 2497. CrossRef
Genome-Wide Analysis of Staphylococcus aureus Sequence Type 72 Isolates Provides Insights Into Resistance Against Antimicrobial Agents and Virulence Potential
Nayab Batool, Amen Shamim, Akhilesh Kumar Chaurasia, Kyeong Kyu Kim
Frontiers in Microbiology.2021;[Epub] CrossRef
A Longitudinal Study of Adult Patients withStaphylococcus aureusBacteremia over 11 Years in Korea
Seong-Ho Choi, Jeongsoo Lee, Jiwon Jung, Eun Sil Kim, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Jun Hee Woo, Yang Soo Kim
Journal of Korean Medical Science.2021;[Epub] CrossRef
Changing characteristics of S. aureus bacteremia caused by PVL-negative, MRSA strain over 11 years
Eunmi Yang, Eunsil Kim, Hyemin Chung, Yun Woo Lee, Seongman Bae, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Ho Choi, Sang-Oh Lee, Yang Soo Kim
Scientific Reports.2021;[Epub] CrossRef
Functional Identification of Serine Hydroxymethyltransferase as a Key Gene Involved in Lysostaphin Resistance and Virulence Potential of Staphylococcus aureus Strains
Nayab Batool, Kwan Soo Ko, Akhilesh Kumar Chaurasia, Kyeong Kyu Kim
International Journal of Molecular Sciences.2020; 21(23): 9135. CrossRef
Sterilization efficiency of pathogen-contaminated cottons in a laundry machine
Yoonjae Shin, Jungha Park, Woojun Park
Journal of Microbiology.2020; 58(1): 30. CrossRef
Clinical S. aureus Isolates Vary in Their Virulence to Promote Adaptation to the Host
Lorena Tuchscherr, Christine Pöllath, Anke Siegmund, Stefanie Deinhardt-Emmer, Verena Hoerr, Carl-Magnus Svensson, Marc Thilo Figge, Stefan Monecke, Bettina Löffler
Toxins.2019; 11(3): 135. CrossRef
Impact of Community-Onset Methicillin-ResistantStaphylococcus aureusonStaphylococcus aureusBacteremia in a Central Korea Veterans Health Service Hospital
Eunsin Bae, Choon Kwan Kim, Jung-Hyun Jang, Heungsup Sung, YounMi Choi, Mi-Na Kim
Annals of Laboratory Medicine.2019; 39(2): 158. CrossRef
Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean
Yae Sung Mun, You Jin Hwang
Antibiotics.2019; 8(4): 202. CrossRef
Emergence of Community-Genotype Methicillin-ResistantStaphylococcus aureusin Korean Hospitals: Clinical Characteristics of Nosocomial Infections by Community-Genotype Strain
Eun-Jeong Joo, Doo Ryeon Chung, So Hyun Kim, Jin Yang Baek, Nam Yong Lee, Sun Young Cho, Young Eun Ha, Cheol-In Kang, Kyong Ran Peck, Jae-Hoon Song
Infection & Chemotherapy.2017; 49(2): 109. CrossRef
Comparison of subsequent infection in methicillin-resistant Staphylococcus aureus nasal carriers between ST72 community-genotype and hospital genotypes: a retrospective cohort study
So Yeon Park, Doo Ryeon Chung, Yu Ri Kang, So Hyun Kim, Sun Young Cho, Young Eun Ha, Cheol-In Kang, Kyong Ran Peck, Jae-Hoon Song
Antimicrobial Resistance & Infection Control.2017;[Epub] CrossRef
Molecular Typing and Resistance Profiles of Vancomycin-IntermediateStaphylococcus aureusin Korea: Results from a National Surveillance Study, 2007-2013
Jung Wook Kim, Gi Su Kang, Jae Il Yoo, Hwa Su Kim, Yeong Seon Lee, Jae-Yon Yu, Kwang-Jun Lee, Chan Park, Il-Hwan Kim
Annals of Clinical Microbiology.2016; 19(4): 88. CrossRef

Research Support, Non-U.S. Gov't

Inhibition of eukaryotic translation by tetratricopeptide-repeat proteins of Orientia tsutsugamushi: Sunyoung Bang , Chan-Ki Min , Na-Young Ha , Myung-Sik Choi , Ik-Sang Kim , Yeon-Sook Kim , Nam-Hyuk Cho; J. Microbiol. 2016;54(2):136-144. Published online February 2, 2016; DOI: https://doi.org/10.1007/s12275-016-5599-5

306 View
0 Download
7 Crossref

Abstract PDF

Orientia tsutsugamushi, an obligate intracellular bacterium, is the causative agent of scrub typhus. The genome of Orientia tsutsugamushi has revealed multiple ORFs encoding tetratricopeptide- repeat (TPR) proteins. The TPR protein family has been shown to be involved in a diverse spectrum of cellular functions such as cell cycle control, transcription, protein transport, and protein folding, especially in eukaryotic cells. However, little is known about the function of the TPR proteins in O. tsutsugamushi. To investigate the potential role of TPR proteins in host-pathogen interaction, two oriential TPR proteins were expressed in E. coli and applied for GSTpull down assay. DDX3, a DEAD-box containing RNA helicase, was identified as a specific eukaryotic target of the TPR proteins. Since the RNA helicase is involved in multiple RNAmodifying processes such as initiation of translation reaction, we performed in vitro translation assay in the presence of GST-TPR fusion proteins by using rabbit reticulocyte lysate system. The TPR proteins inhibited in vitro translation of a reporter luciferase in a dose dependent manner whereas the GST control proteins did not. These results suggested TPR proteins of O. tsutsugamushi might be involved in the modulation of eukarytotic translation through the interaction with DDX3 RNA helicase after secretion into host cytoplasm.

Citations

Citations to this article as recorded by

Orientia tsutsugamushi: comprehensive analysis of the mobilome of a highly fragmented and repetitive genome reveals the capacity for ongoing lateral gene transfer in an obligate intracellular bacterium
Suparat Giengkam, Chitrasak Kullapanich, Jantana Wongsantichon, Haley E. Adcox, Joseph J. Gillespie, Jeanne Salje, Alfredo G. Torres
mSphere.2023;[Epub] CrossRef
The inside scoop: Comparative genomics of two intranuclear bacteria, “Candidatus Berkiella cookevillensis” and “Candidatus Berkiella aquae”
Destaalem T. Kidane, Yohannes T. Mehari, Forest C. Rice, Brock A. Arivett, John H. Gunderson, Anthony L. Farone, Mary B. Farone, Daniel E. Voth
PLOS ONE.2022; 17(12): e0278206. CrossRef
DciA Helicase Operators Exhibit Diversity across Bacterial Phyla
Helen C. Blaine, Joseph T. Burke, Janani Ravi, Christina L. Stallings, Michael J. Federle
Journal of Bacteriology.2022;[Epub] CrossRef
Cells within cells: Rickettsiales and the obligate intracellular bacterial lifestyle
Jeanne Salje
Nature Reviews Microbiology.2021; 19(6): 375. CrossRef
Bioinformatic Exploration of Metal-Binding Proteome of Zoonotic Pathogen Orientia tsutsugamushi
Dixit Sharma, Ankita Sharma, Birbal Singh, Shailender Kumar Verma
Frontiers in Genetics.2019;[Epub] CrossRef
Piscirickettsia salmonis Cryptic Plasmids: Source of Mobile DNA and Virulence Factors
Javiera Ortiz-Severín, Dante Travisany, Alejandro Maass, Francisco P. Chávez, Verónica Cambiazo
Pathogens.2019; 8(4): 269. CrossRef
An Update on Host-Pathogen Interplay and Modulation of Immune Responses during Orientia tsutsugamushi Infection
Fabián E. Díaz, Katia Abarca, Alexis M. Kalergis
Clinical Microbiology Reviews.2018;[Epub] CrossRef

Journal Article

Regulation of HBV-specific CD8+ T cell-mediated inflammation is diversified in different clinical presentations of HBV infection: Colin M. Dinney , Lu-Dong Zhao , Charles D. Conrad , Jay M. Duker , Richard O. Karas , Zhibin Hu , Michele A. Hamilton , Thomas R. Gillis , Thomas M. Parker , Bing Fan , Andrew H. Advani , Fred B. Poordad , Paulette L. Fauceglia , Kathrin M. Kirsch , Peter T. Munk , Marc P. Ladanyi , Bernard A. Bochner , Justin A. Bekelman , Carla M. Grandori , James C. Olson , Ronald D. Lechan , Ghassan M.A. Abou , Mark A. Goodarzi; J. Microbiol. 2015;53(10):718-724. Published online October 2, 2015; DOI: https://doi.org/10.1007/s12275-015-5314-y

348 View
0 Download
23 Crossref

Abstract

Chronic HBV infection is the leading cause of liver cirrhosis and hepatic cancer, but the individual responses toward HBV infection are highly variable, ranging from asymptomatic to chronic active hepatitis B inflammation. In this study, we hypothesized that the different individual responses to HBV infection was associated with differences in HBV-specific CD8+ T cell-mediated inflammation and cytotoxicity. Blood samples were collected from subjects with asymptomatic HBV-infection, subjects undergoing active chronic HBV flares (active CHB), and subjects with HBV-infected hepatocellular carcinoma (HBV-HCC). By tetramer staining, we found that all three groups had similar frequencies of HBVspecific CD8+ T cells. However, after HBV peptide stimulation, the HBV-specific CD8+ T cells in asymptomatic subjects had significantly stronger interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α), and CD107a expression than those in active CHB and HBV-HCC patients. Examination of surface marker expression revealed that the PD-1-Tim-3- double-negative cell population was the main contributor to HBV-specific inflammation. In active CHB patients and HBV-HCC patients, however, the frequencies of activated PD-1-Tim-3- cells were significantly reduced. Moreover, the serum HBV DNA titer was not correlated with the frequencies of HBV-specific CD8+ T cells but was inversely correlated with the frequencies of IFN-g-expressing and CD107a-express cells in response to HBV stimulation. Together, our data demonstrated that the status of HBVspecific CD8+ T cell exhaustion was associated with different clinical outcomes of chronic HBV infection.

Citations

Citations to this article as recorded by

The combination of CD8 and TIM3 expression predicts survival outcomes in hepatocellular carcinoma
Takayuki Noma, Yuma Wada, Masaaki Nishi, Hiroki Teraoku, Shinichiro Yamada, Yu Saito, Tetsuya Ikemoto, Yuji Morine, Mitsuo Shimada
The Journal of Medical Investigation.2025; 72(3.4): 354. CrossRef
The Adaptive Immune Response in Hepatitis B Virus-Associated Hepatocellular Carcinoma Is Characterized by Dysfunctional and Exhausted HBV-Specific T Cells
Malene Broholm, Anne-Sofie Mathiasen, Ása Didriksen Apol, Nina Weis
Viruses.2024; 16(5): 707. CrossRef
Tumor-associated macrophages and CD8+ T cells: dual players in the pathogenesis of HBV-related HCC
Muhammad Naveed Khan, Binli Mao, Juan Hu, Mengjia Shi, Shunyao Wang, Adeel Ur Rehman, Xiaosong Li
Frontiers in Immunology.2024;[Epub] CrossRef
The CD8+ T cell exhaustion mechanisms in chronic hepatitis B infection and immunotherapeutic strategies: a systematic review
Esmaeil Allahmoradi, Ramtin Mohammadi, Peyman Kheirandish Zarandi, Seyed Moayed Alavian, Mohammad Heiat
Expert Review of Clinical Immunology.2023; 19(6): 671. CrossRef
Increase in Serum Soluble Tim-3 Level Is Related to the Progression of Diseases After Hepatitis Virus Infection
Lingli Chen, Xiaomei Yu, Chunyan Lv, Yaping Dai, Tao Wang, Shaoxiong Zheng, Yuan Qin, Xiumei Zhou, Yigang Wang, Hao Pei, Hongming Fang, Biao Huang
Frontiers in Medicine.2022;[Epub] CrossRef
Programmed death 1 expressing CD8+CXCR5+ follicular T cells constitute effector rather than exhaustive phenotype in patients with chronic hepatitis B
Arshi Khanam, Lydia S. Y. Tang, Shyam Kottilil
Hepatology.2022; 75(3): 690. CrossRef
Insights into the Relationship between Pentraxin-3 and Cancer
Maria Bogdan, Andreea-Daniela Meca, Adina Turcu-Stiolica, Carmen Nicoleta Oancea, Roxana Kostici, Marin Valeriu Surlin, Cristina Florescu
International Journal of Molecular Sciences.2022; 23(23): 15302. CrossRef
Hepatitis B Flare in Hepatitis B e Antigen-Negative Patients: A Complicated Cascade of Innate and Adaptive Immune Responses
Ming-Ling Chang, Yun-Fan Liaw
International Journal of Molecular Sciences.2022; 23(3): 1552. CrossRef
Soluble programmed cell death-1 predicts hepatocellular carcinoma development during nucleoside analogue treatment
Ritsuzo Kozuka, Masaru Enomoto, Minh Phuong Dong, Hoang Hai, Le Thi Thanh Thuy, Naoshi Odagiri, Kanako Yoshida, Kohei Kotani, Hiroyuki Motoyama, Etsushi Kawamura, Atsushi Hagihara, Hideki Fujii, Sawako Uchida-Kobayashi, Akihiro Tamori, Norifumi Kawada
Scientific Reports.2022;[Epub] CrossRef
The Value of Immune System in the Progression of Chronic Hepatitis B
甜甜武
Advances in Clinical Medicine.2021; 11(11): 5386. CrossRef
Identification of the association between HBcAg-specific T cell and viral control in chronic HBV infection using a cultured ELISPOT assay
Chengcong Chen, Xiaotao Jiang, Xuan Liu, Ling Guo, Weibin Wang, Shuqin Gu, Chunhua Wen, Xuan Yi, Libo Tang, Yongyin Li
Journal of Leukocyte Biology.2021; 109(2): 455. CrossRef
Molecular signature of hepatitis B virus regulation by interferon‐γ in primary human hepatocytes
Takuto Nosaka, Tatsushi Naito, Hidetaka Matsuda, Masahiro Ohtani, Katsushi Hiramatsu, Tomoyuki Nemoto, Tsutomu Nishizawa, Hiroaki Okamoto, Yasunari Nakamoto
Hepatology Research.2020; 50(3): 292. CrossRef
Serum pentraxin 3 as a biomarker of hepatocellular carcinoma in chronic hepatitis B virus infection
Huan Deng, Xiude Fan, Xiaoyun Wang, Lu Zeng, Kun Zhang, Xiaoge Zhang, Na Li, Qunying Han, Yi Lv, Zhengwen Liu
Scientific Reports.2020;[Epub] CrossRef
Immune Checkpoints in Viral Infections
Huiming Cai, Ge Liu, Jianfeng Zhong, Kai Zheng, Haitao Xiao, Chenyang Li, Xun Song, Ying Li, Chenshu Xu, Haiqiang Wu, Zhendan He, Qinchang Zhu
Viruses.2020; 12(9): 1051. CrossRef
PRDM1 levels are associated with clinical diseases in chronic HBV infection and survival of patients with HBV-related hepatocellular carcinoma
Na Li, Xiude Fan, Xiaoyun Wang, Huan Deng, Kun Zhang, Xiaoge Zhang, Ye Wang, Qunying Han, Yi Lv, Zhengwen Liu
International Immunopharmacology.2019; 73: 156. CrossRef
Hepatitis B virus-specific effector CD8+ T cells are an important determinant of disease prognosis: A meta-analysis
Juzeng Zheng, Zhanfan Ou, Yilun Xu, Ziqiang Xia, Xianfan Lin, Sisi Jin, Yang Liu, Jinming Wu
Vaccine.2019; 37(18): 2439. CrossRef
PRDM1 rs1010273 polymorphism is associated with overall survival of patients with hepatitis B virus-related hepatocellular carcinoma
Na Li, Xiude Fan, Xiaoyun Wang, Huan Deng, Kun Zhang, Xiaoge Zhang, Qunying Han, Yi Lv, Zhengwen Liu
Immunology Letters.2019; 213: 39. CrossRef
Association of viral hepatitis and bipolar disorder: a nationwide population-based study
Lee-Won Chong, Chih-Chao Hsu, Chang-Yin Lee, Ruey-Hwang Chou, Cheng-Li Lin, Kuang-Hsi Chang, Yi-Chao Hsu
Journal of Translational Medicine.2018;[Epub] CrossRef
Tim-3 expression and its role in hepatocellular carcinoma
Feifei Liu, Yanning Liu, Zhi Chen
Journal of Hematology & Oncology.2018;[Epub] CrossRef
Novel Immunotherapy Strategies for Hepatobiliary Cancers
Thomas T DeLeon, Yumei Zhou, Bolni M Nagalo, Raquel T Yokoda, Daniel H Ahn, Ramesh K Ramanathan, Marcela A Salomao, Bashar A Aqel, Amit Mahipal, Tanios S Bekaii-Saab, Mitesh J Borad
Immunotherapy.2018; 10(12): 1077. CrossRef
Association of LTBR polymorphisms with chronic hepatitis B virus infection and hepatitis B virus-related hepatocellular carcinoma
Qianqian Zhu, Na Li, Fang Li, Jiao Sang, Huan Deng, Qunying Han, Yi Lv, Chunyan Li, Zhengwen Liu
International Immunopharmacology.2017; 49: 126. CrossRef
The development of hepatocarcinoma after long-term antivirus treatment of Chinese patients with chronic hepatitis B virus infection: Incidence, long-term outcomes and predictive factors
Zhi-Qin Li, Chun-Ling Hu, Ping Yu, Xin-Yu Gu, Jia-Jia Zhang, Hua Li, Hong-Yu Zhang, Jun Lv, Yan-Min Liu, Qing-Lei Zeng, Jing-Ya Yan, Zu-Jiang Yu, Yi Zhang
Clinics and Research in Hepatology and Gastroenterology.2017; 41(3): 311. CrossRef
Impaired Subset Progression and Polyfunctionality of T Cells in Mice Exposed to Methamphetamine during Chronic LCMV Infection
Uma Sriram, Beth L. Hill, Jonathan M. Cenna, Larisa Gofman, Nicole C. Fernandes, Bijayesh Haldar, Raghava Potula, Anil Kumar
PLOS ONE.2016; 11(10): e0164966. CrossRef

First
Prev
Page of 4
Next
Last

Search