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- The transcription factor Cas5 suppresses hyphal morphogenesis during yeast-form growth in Candida albicans
- Jong-Myeong Kim , Hye Yun Moon , Dong Wook Lee , Hyun Ah Kang , Jeong-Yoon Kim
- J. Microbiol. 2021;59(10):911-919. Published online September 7, 2021
- DOI: https://doi.org/10.1007/s12275-021-1326-y
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- 2 Web of Science
- 1 Crossref
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Abstract
-
Candida albicans is an opportunistic human pathogen that
exists as yeast, hyphal or pseudohyphal forms depending on
pH, nutrients, and temperature. The morphological transition
from yeast to hyphae, which is required for the complete virulence
of C. albicans, is controlled by many transcription factors
that activate or repress hypha-specific genes. The C. albicans
transcriptional factor Cas5, a key regulator of genes involved
in cell wall integrity, affects the susceptibility of C. albicans
to fluconazole, an inhibitor of ergosterol synthesis. In
this study, we found that deletion of CAS5 in C. albicans decreased
the expression levels of a set of ergosterol biosynthesis
genes, such as ERG2, ERG3, ERG5, ERG6, ERG11, and ERG24,
result
ing in the accumulation of lanosterol and zymosterol, which are intermediate metabolites in the ergosterol biosynthesis pathway. Interestingly, it was observed that the cas5Δ/Δ mutant could not maintain the yeast form under non-hyphainducing conditions, while the CAS5-overexpressing cells could not form hyphae under hypha-inducing conditions. Consistent with these observations, the cas5Δ/Δ mutant highly expressed hypha-specific genes, ALS3, ECE1, and HWP1, under non-hypha-inducing conditions. In addition, CAS5 transcription was significantly downregulated immediately after hyphal initiation in the wild-type strain. Furthermore, the cas5Δ/Δ mutant reduced the transcription of NRG1, which encodes a major repressor of hyphal morphogenesis, while Cas5 overexpression increased the transcription of NRG1 under hyphainducing conditions. Collectively, this study suggests the potential role of Cas5 as a repressor of hypha-specific genes during yeast-form growth of C. albicans. -
Citations
Citations to this article as recorded by
- The Role of Sfp1 in Candida albicans Cell Wall Maintenance
Che-Kang Chang, Min-Chi Yang, Hsueh-Fen Chen, Yi-Ling Liao, Chung-Yu Lan
Journal of Fungi.2022; 8(11): 1196. CrossRef
- The Role of Sfp1 in Candida albicans Cell Wall Maintenance
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