Journal Articles
- Biocontrol activity of volatile organic compounds from Streptomyces alboflavus TD-1 against Aspergillus flavus growth and aflatoxin production
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Mingguan Yang , Laifeng Lu , Jing Pang , Yiling Hu , Qingbin Guo , Zhenjing Li , Shufen Wu , Huanhuan Liu , Changlu Wang
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J. Microbiol. 2019;57(5):396-404. Published online May 6, 2019
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DOI: https://doi.org/10.1007/s12275-019-8517-9
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Abstract
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Aspergillus flavus is a saprophytic fungus that contaminates
crops with carcinogenic aflatoxin. In the present work, the
antifungal effects of volatile organic compounds (VOCs) from
Streptomyces alboflavus TD-1 against A. flavus were investigated.
VOCs from 8-day-old wheat bran culture of S. alboflavus
TD-1 displayed strong inhibitory effects against mycelial
growth, sporulation, and conidial germination of A.
flavus. Severely misshapen conidia and hyphae of A. flavus
were observed by scanning electron microscopy after exposure
to VOCs for 6 and 12 h, respectively. Rhodamine 123
staining of mitochondria indicated that mitochondria may
be a legitimate antifungal target of the VOCs from S. alboflavus
TD-1. Furthermore, the VOCs effectively inhibited
aflatoxin B1 production by downregulating genes involved
in aflatoxin biosynthesis. Dimethyl trisulfide and benzenamine
may play important roles in the suppression of A. flavus
growth and production of aflatoxin. The results indicate
that VOCs from S. alboflavus TD-1 have tremendous potential
to be developed as a useful bio-pesticide for controlling
A. flavus.
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Citations
Citations to this article as recorded by

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Fusarium kuroshium
and
Fusarium solani
and promote the growth of
Arabidopsis thaliana
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Efficacy of volatile compounds from
Streptomyces philanthi
RL‐1‐178 as a biofumigant for controlling growth and aflatoxin production of the two aflatoxin‐producing fungi on stored soybean seeds
S. Boukaew, P. Prasertsan
Journal of Applied Microbiology.2020; 129(3): 652. CrossRef - Microenvironmental Interplay Predominated by Beneficial Aspergillus Abates Fungal Pathogen Incidence in Paddy Environment
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- Characterization of the velvet regulators in Aspergillus flavus
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Tae-Jin Eom , Heungyun Moon , Jae-Hyuk Yu , Hee-Soo Park
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J. Microbiol. 2018;56(12):893-901. Published online October 25, 2018
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DOI: https://doi.org/10.1007/s12275-018-8417-4
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Abstract
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Fungal development and secondary metabolism are closely
associated via the activities of the fungal NK-kB-type velvet
regulators that are highly conserved in filamentous fungi.
Here, we investigated the roles of the velvet genes in the aflatoxigenic
fungus Aspergillus flavus. Distinct from other Aspergillus
species, the A. flavus genome contains five velvet genes,
veA, velB, velC, velD, and vosA. The deletion of velD blocks
the production of aflatoxin B1, but does not affect the formation
of sclerotia. Expression analyses revealed that vosA and
velB mRNAs accumulated at high levels during the late phase
of asexual development and in conidia. The absence of vosA
or velB decreased the content of conidial trehalose and the
tolerance of conidia to the thermal and UV stresses. In addition,
double mutant analyses demonstrated that VosA and
VelB play an inter-dependent role in trehalose biosynthesis
and conidial stress tolerance. Together with the findings of
previous studies, the results of the present study suggest that
the velvet regulators play the conserved and vital role in sporogenesis,
conidial trehalose biogenesis, stress tolerance, and
aflatoxin biosynthesis in A. flavus.
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J. Microbiol. 2018;56(5):356-364. Published online May 2, 2018
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DOI: https://doi.org/10.1007/s12275-018-7349-3
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Abstract
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Aspergillus flavus often invade many important corps and
produce harmful aflatoxins both in preharvest and during
storage stages. The regulation mechanism of aflatoxin biosynthesis
in this fungus has not been well explored mainly
due to the lack of an efficient transformation method for
constructing a genome-wide gene mutant library. This challenge
was resolved in this study, where a reliable and efficient
Agrobacterium tumefaciens-mediated transformation (ATMT)
protocol for A. flavus NRRL 3357 was established. The results
showed that removal of multinucleate conidia, to collect
a homogenous sample of uninucleate conidia for use as the
transformation material, is the key step in this procedure.
A. tumefaciens strain AGL-1 harboring the ble gene for zeocin
resistance under the control of the gpdA promoter from
A. nidulans is suitable for genetic transformation of this fungus.
We successfully generated A. flavus transformants with
an efficiency of ~ 60 positive transformants per 106 conidia
using our protocol. A small-scale insertional mutant library
(~ 1,000 mutants) was constructed using this method and
the resulting several mutants lacked both production of conidia
and aflatoxin biosynthesis capacity. Southern blotting
analysis demonstrated that the majority of the transformants
contained a single T-DNA insert on the genome. To the best
of our knowledge, this is the first report of genetic transformation
of A. flavus via ATMT and our protocol provides an
effective tool for construction of genome-wide gene mutant
libraries for functional analysis of important genes in A.
flavus.
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Citations
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- Isolation and characterization of Aspergillus flavus strains in China
-
Firew Tafesse Mamo , Bo Shang , Jonathan Nimal Selvaraj , Yan Wang , Yang Liu
-
J. Microbiol. 2018;56(2):119-127. Published online February 2, 2018
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DOI: https://doi.org/10.1007/s12275-018-7144-1
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43
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19
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Abstract
-
Important staple foods (peanuts, maize and rice) are susceptible
to contamination by aflatoxin (AF)-producing fungi such
as Aspergillus flavus. The objective of this study was to explore
non-aflatoxin-producing (atoxigenic) A. flavus strains
as biocontrol agents for the control of AFs. In the current
study, a total of 724 A. flavus strains were isolated from different
regions of China. Polyphasic approaches were utilized
for species identification. Non-aflatoxin and non-cyclopiazonic
acid (CPA)-producing strains were further screened
for aflatoxin B1 (AFB1) biosynthesis pathway gene clusters
using a PCR assay. Strains lacking an amplicon for the regulatory
gene aflR were then analyzed for the presence of the
other 28 biosynthetic genes. Only 229 (32%) of the A. flavus
strains were found to be atoxigenic. Smaller (S) sclerotial phenotypes
were dominant (51%) compared to large (L, 34%) and
non-sclerotial (NS, 15%) phenotypes. Among the atoxigenic
strains, 24 strains were PCR-negative for the fas-1 and aflJ
genes. Sixteen (67%) atoxigenic A. flavus strains were PCRnegative
for 10 or more of the biosynthetic genes. Altogether,
18 new PCR product patterns were observed, indicating great
diversity in the AFB1 biosynthesis pathway. The current study
demonstrates that many atoxigenic A. flavus strains can be
isolated from different regions of China. In the future laboratory
as well as field based studies are recommended to test
these atoxigenic strains as biocontrol agents for aflatoxin
contamination.
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Research Support, Non-U.S. Gov'ts
- Molecular Characterization of Atoxigenic Aspergillus flavus Isolates Collected in China
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Dandan Wei , Lu Zhou , Jonathan Nimal Selvaraj , Chushu Zhang , Fuguo Xing , Yueju Zhao , Yan Wang , Yang Liu
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J. Microbiol. 2014;52(7):559-565. Published online May 30, 2014
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DOI: https://doi.org/10.1007/s12275-014-3629-8
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53
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25
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Abstract
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Aspergillus flavus strains were isolated from peanut fields of Liaoning, Shandong, Hubei and Guangdong Provinces in China, and identified through phenotypic and molecular approaches. Of the 323 A. flavus strains isolated, 76 strains did not produce aflatoxins detectable by UPLC. The incidence of atoxigenic A. flavus strains decreased with increase in temperature and increased with increase in latitude in different geographical locations. Amplification of all the aflatoxin genes in the aflatoxin gene cluster in the atoxigenic isolates showed that there were 25 deletion patterns (A-Y), with 22 deletion patterns identified for the first time. Most of the atoxigenic A. flavus isolates with gene deletions (97%) had deletions in at least one of the four genes (aflT, nor-1, aflR, and hypB), indicating that these four genes could be targeted for rapid identification of atoxigenic strains. The atoxigenic isolates with gene deletions, especially the isolates with large deletions, are potential candidates for aflatoxin control.
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- Aflatoxin profiles of Aspergillus flavus isolates in Sudanese fungal rhinosinusitis
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CABI Agriculture and Bioscience.2024;[Epub] CrossRef - The biodiversity of Aspergillus flavus in stored rice grain leads to a decrease in the overall aflatoxin B1 production in these species
Song Tan, Fang Ma, Yajie Wu, Yuancheng Xu, Ajuan Niu, Yuping Chen, Guangyu Wang, Weifen Qiu
International Journal of Food Microbiology.2023; 406: 110416. CrossRef - Biocontrol efficacy of atoxigenic Aspergillus flavus strains against aflatoxin contamination in peanut field in Guangdong province, South China
Firew Tafesse Mamo, Bo Shang, Jonathan Nimal Selvaraj, Yongquan Zheng, Yang Liu
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Qi Sun, Bo Shang, Ling Wang, Zhisong Lu, Yang Liu
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Lu Zhou, Dan-Dan Wei, Jonathan Nimal Selvaraj, Bo Shang, Chu-Shu Zhang, Fu-Guo Xing, Yue-Ju Zhao, Yan Wang, Yang Liu
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Perng-Kuang Chang, Leslie L. Scharfenstein, Cesar D. Solorzano, Hamed K. Abbas, Sui-Sheng T. Hua, Walker A. Jones, Robert M. Zablotowicz
International Journal of Food Microbiology.2015; 200: 66. CrossRef - Diversity, Saccharification Capacity, and Toxigenicity Analyses of Fungal Isolates in Nuruk
Min Sik Kim, Sinil Kim, Byeong-Seok Ha, Hye-Young Park, Seong-Yeol BaeK, Soo-Hwan Yeo, Hyeon-Su Ro
The Korean Journal of Mycology.2014; 42(3): 191. CrossRef
- The Proportion of Non-Aflatoxigenic Strains of the Aspergillus flavus/oryzae Complex from Meju by Analyses of the Aflatoxin Biosynthetic Genes
-
Seung-Beom Hong , Mina Lee , Dae-Ho Kim , Soo-Hyun Chung , Hyeon-Dong Shin , Robert A. Samson
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J. Microbiol. 2013;51(6):766-772. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3128-3
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42
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13
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Abstract
-
Strains of the Aspergillus flavus/oryzae complex are frequently
isolated from meju, a fermented soybean product, that is used
as the starting material for ganjang (soy sauce) and doenjang
(soybean paste) production. In this study, we examined the
aflatoxin producing capacity of A. flavus/oryzae strains isolated
from meju. 192 strains of A. flavus/oryzae were isolated
from more than 100 meju samples collected from diverse
regions of Korea from 2008 to 2011, and the norB-cypA,
omtA, and aflR genes in the aflatoxin biosynthesis gene
cluster were analyzed. We found that 178 strains (92.7%)
belonged to non-aflatoxigenic group (Type I of norB-cypA,
IB-L-B-, IC-AO, or IA-L-B- of omtA, and AO type of aflR),
and 14 strains (7.3%) belonged to aflatoxin-producible group
(Type II of norB-cypA, IC-L-B+/B- or IC-L-B+ of omtA, and
AF type of aflR). Only 7 strains (3.6%) in the aflatoxin-producible
group produced aflatoxins on Czapek yeast-extract
medium. The aflatoxin-producing capability of A. flavus/
oryzae strains from other sources in Korea were also investigated,
and 92.9% (52/56) strains from air, 93.9% (31/33)
strains from rice straw, 91.7% (11/12) strains from soybean,
81.3% (13/16) strains from corn, 82% (41/50) strains from
peanut, and 73.2% (41/56) strains from arable soil were included
in the non-aflatoxigenic group. The proportion of
non-aflatoxigenicity of meju strains was similar to that of
strains from soybean, air and rice straw, all of which have
an effect on the fermentation of meju. The data suggest that
meju does not have a preference for non-aflatoxigenic or aflatoxin-producible strains of A. flavus/oryzae from the
environment of meju. The non-aflatoxigenic meju strains
are proposed to be named A. oryzae, while the meju strains
that can produce aflatoxins should be referred to A. flavus
in this study.
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