Journal of Microbiology

Journal Articles

The role of Jacalin-related lectin gene AOL_s00083g511 in the development and pathogenicity of the nematophagous fungus Arthrobotrys oligospora: Xinyuan Dong , Jiali Si , Guanghui Zhang , Zhen Shen , Li Zhang , Kangliang Sheng , Jingmin Wang , Xiaowei Kong , Xiangdong Zha , Yongzhong Wang; J. Microbiol. 2021;59(8):736-745. Published online July 5, 2021; DOI: https://doi.org/10.1007/s12275-021-1029-4

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Arthrobotrys oligospora is a model species of nematophagous fungi and has great potential for the biological control of nematode diseases. Lectin is a protein that binds to carbohydrates and their complexes with high specificity, which mediates recognition events in various physiological and pathological processes. This study aimed to investigate the role of the Jacalin-related lectin (JRL) gene, AOL_s00083g511, in A. oligospora development. Through a homology recombination approach, we obtained the AOL_s00083g511 knockout mutant strain (Δg511). Next, the biological characteristics of the Δg511 mutant strain, including growth rate, conidia germination rate, adaptation to environmental stresses, and nematocidal activity, were compared with those of the wild-type (WT) strain. The results showed that the JRL gene AOL_ s00083g511 did not affect fungal growth, conidia germination, 3D-trap formation, and the ability of A. oligospora to prey on nematodes significantly. We speculate that this phenomenon may be caused by a loss of the key β1–β2 loops in the AOL_ s00083g511-encoded JRL domain and an intrinsic genetic compensation of AOL_s00083g511 in this fungus. The growth rates of both strains on high salt or surfactant media were similar; however, in the strong oxidation medium, the growth rate of the Δg511 mutant was significantly lower than that of the WT strain, indicating that AOL_s00083g511 might play a role in oxidative stress resistance. These findings provide a basis for further analysis of the related functions of the JRL gene in A. oligospora and their potential roles in the biological control of nematodes in the future.

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Function discovery of a non-ribosomal peptide synthetase-like encoding gene in the nematode-trapping fungus Arthrobotrys oligospora
Tiantian Gu, Hengqian Lu, Huiwen Liu, Guanghui Zhang, Yongzhong Wang
Frontiers in Microbiology.2023;[Epub] CrossRef
The fucose-specific lectin gene AOL_s00054g276 affects trap formation and nematocidal activity of the nematophagous fungus Arthrobotrys oligospora
Jiali Si, Xinyuan Dong, Guanghui Zhang, Hengqian Lu, Kaijing Tang, Li Zhang, Xiaowei Kong, Kangliang Sheng, Jingmin Wang, Xiangdong Zha, Yongzhong Wang
FEMS Microbiology Letters.2022;[Epub] CrossRef
Phospholipase C (AoPLC2) regulates mycelial development, trap morphogenesis, and pathogenicity of the nematode-trapping fungus Arthrobotrys oligospora
Meihua Xie, Ni Ma, Na Bai, Meichen Zhu, Ke-Qin Zhang, Jinkui Yang
Journal of Applied Microbiology.2022; 132(3): 2144. CrossRef

Molecular characterization of Hsf1 as a master regulator of heat shock response in the thermotolerant methylotrophic yeast Ogataea parapolymorpha: Jin Ho Choo , Su-Bin Lee , Hye Yun Moon , Kun Hwa Lee , Su Jin Yoo , Keun Pil Kim , Hyun Ah Kang; J. Microbiol. 2021;59(2):151-163. Published online February 1, 2021; DOI: https://doi.org/10.1007/s12275-021-0646-2

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Abstract

Ogataea parapolymorpha (Hansenula polymorpha DL-1) is a thermotolerant methylotrophic yeast with biotechnological applications. Here, O. parapolymorpha genes whose expression is induced in response to heat shock were identified by transcriptome analysis and shown to possess heat shock elements (HSEs) in their promoters. The function of O. parapolymorpha HSF1 encoding a putative heat shock transcription factor 1 (OpHsf1) was characterized in the context of heat stress response. Despite exhibiting low sequence identity (26%) to its Saccharomyces cerevisiae homolog, OpHsf1 harbors conserved domains including a DNA binding domain (DBD), domains involved in trimerization (TRI), transcriptional activation (AR1, AR2), transcriptional repression (CE2), and a C-terminal modulator (CTM) domain. OpHSF1 could complement the temperature sensitive (Ts) phenotype of a S. cerevisiae hsf1 mutant. An O. parapolymorpha strain with an H221R mutation in the DBD domain of OpHsf1 exhibited significantly retarded growth and a Ts phenotype. Intriguingly, the expression of heat-shock-protein‒coding genes harboring HSEs was significantly decreased in the H221R mutant strain, even under non-stress conditions, indicating the importance of the DBD for the basal growth of O. parapolymorpha. Notably, even though the deletion of C-terminal domains (ΔCE2, ΔAR2, ΔCTM) of OpHsf1 destroyed complementation of the growth defect of the S. cerevisiae hsf1 strain, the C-terminal domains were shown to be dispensable in O. parapolymorpha. Overexpression of OpHsf1 in S. cerevisiae increased resistance to transient heat shock, supporting the idea that OpHsf1 could be useful in the development of heatshock‒ resistant yeast host strains.

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A comprehensive review and comparison of L-tryptophan biosynthesis in Saccharomyces cerevisiae and Escherichia coli
Xinru Ren, Yue Wei, Honglu Zhao, Juanjuan Shao, Fanli Zeng, Zhen Wang, Li Li
Frontiers in Bioengineering and Biotechnology.2023;[Epub] CrossRef
Heat shock in Cronobacter sakazakii induces direct protection and cross-protection against simulated gastric fluid stress
Hongmei Niu, MingzheYang, Yonghua Qi, Yangtai Liu, Xiang Wang, Qingli Dong
Food Microbiology.2022; 103: 103948. CrossRef
A review of yeast: High cell-density culture, molecular mechanisms of stress response and tolerance during fermentation
Dongxu Shen, Xiaoli He, Peifang Weng, Yanan Liu, Zufang Wu
FEMS Yeast Research.2022;[Epub] CrossRef

Development of a strategy for the screening of α-glucosidase-producing microorganisms: Bo Zhou+ , Nan Huang+ , Wei Zeng+ , Hao Zhang , Guiguang Chen , Zhiqun Liang; J. Microbiol. 2020;58(2):163-172. Published online January 29, 2020; DOI: https://doi.org/10.1007/s12275-020-9267-4

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Abstract

α-Glucosidase is a crucial enzyme for the production of isomaltooligosaccharide. In this study, a novel method comprising eosin Y (EY) and α-D-methylglucoside (AMG) in glass plates was tested for the primary screening of α-glucosidaseproducing strains. First, α-glucosidase-producing Aspergillus niger strains were selected on plates containing EY and AMG based on transparent zone formation resulting from the solubilization of EY by the hydrolyzed product. Conventional
methods
that use trypan blue (TB) and p-nitrophenyl-α-Dglucopyranoside (pPNP) as indicators were then compared with the new strategy. The results showed that EY-containing plates provide the advantages of low price and higher specificity for the screening of α-glucosidase-producing strains. We then evaluated the correlation between the hydrolytic activity of α-glucosidase and diffusion distance, and found that good linearity could be established within a 6–75 U/ml enzyme concentration range. Finally, the hydrolytic and transglycosylation activities of α-glucosidase obtained from the target isolates were determined by EY plate assay and 3,5- dinitrosalicylic acid-Saccharomyces cerevisiae assay, respectively. The results showed that the diameter of the transparent zone varied among isolates was positively correlated with α-glucosidase hydrolytic activity, while good linearity could also be established between α-glucosidase transglycosylation activity and non-fermentable reducing sugars content. With this strategy, 7 Aspergillus niger mutants with high yield of α-glucosidase from 200 obvious single colonies on the primary screen plate were obtained.

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Purification, characterization of a novel α-glucosidase from Debaryomyces hansenii strain MCC 0202 and chromatographic separation for high purity isomalto-oligosaccharides production
Saravanan Rengarajan, Rameshthangam Palanivel
Process Biochemistry.2024; 136: 109. CrossRef
Development of a PMA‐LAMP visual detection assay for viable Cronobacter sakazakii
Qiming Chen, Yang Yu, Xiaodi Chen, Fangming Tu, Peng Wang, Junyi Huang, Zhanmin Liu
International Journal of Dairy Technology.2024; 77(2): 427. CrossRef
Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation
Chunxu Jiang, Han Wang, Menghan Liu, Li Wang, Ruwen Yang, Peng Wang, Zongmei Lu, Yong Zhou, Zhiming Zheng, Genhai Zhao
Applied Microbiology and Biotechnology.2022; 106(21): 6993. CrossRef
Cloning and characterization of a recombinant α-glucosidase from Ensifer adhaerens NBRC 100388 and evaluation of its glucosyl transfer activity
Tatsuya Suzuki, Miyu Fukaya, Kazuki Takahashi, Michiki Takeuchi, Ryotaro Hara, Jun Ogawa, Makoto Ueda
Biocatalysis and Agricultural Biotechnology.2020; 30: 101837. CrossRef

Production of an Endoinulinase from Aspergillus niger AUMC 9375, by Solid State Fermentation of Agricultural Wastes, with Purification and Characterization of the Free and Immobilized Enzyme: Manal M. Housseiny; J. Microbiol. 2014;52(5):389-398. Published online May 9, 2014; DOI: https://doi.org/10.1007/s12275-014-3561-y

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Abstract

Two different substrates, sunflower (Helianthus annuus L.) tubers and lettuce (Lactuca sativa) roots, were tested. Using a mixture of both wastes resulted in higher production of endoinulinase than either waste alone. Also, ten fungal spe-cies grown on these substrates as inexpensive, carbon sour-ces were screened for the best production of endoinulinase activities. Of these, Aspergillus niger AUMC 9375 was the most productive, when grown on the mixture using a 6:1 w/w ratio of sun flower: lettuce, and yielded the highest levels of inulinase at 50% moisture, 30°C, pH 5.0, with seven days of incubation, and with yeast extract as the best nitrogen source. Inulinase was purified to homogeneity by ion-exchange chro-matography and gel-filtration giving a 51.11 fold purification. The mixture of sunflower tubers and lettuce roots has poten-tial to be an effective and economical substrate for inulinase production. Inulinase was successfully immobilized with an immobilization yield of 71.28%. After incubation for 2 h at 60°C, the free enzyme activity decreased markedly to 10%, whereas that of the immobilized form decreased only to 87%. A reusability test demonstrated the durability of the immo-bilized inulinase for 10 cycles and in addition, that it could be stored for 32 days at 4°C. These results indicate that this inulinase, in the immobilized form, is a potential candidate for large-scale production of high purity fructose syrups.

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Production of β-mannanase, inulinase, and oligosaccharides from coffee wastes and extracts
Selin Basmak, Irfan Turhan
International Journal of Biological Macromolecules.2024; 261: 129798. CrossRef
Sustainable inulinase enzyme production from novel strain Fusarium parceramosum with mixed biomass substrates of rice husk and banana shoot through solid-state fermentation
Shreya Hegde, Ramananda Bhat M, Subbalaxmi Selvaraj
Biomass Conversion and Biorefinery.2024;[Epub] CrossRef
Bio-utilization of agricultural residue banana plant shoot through solid state fermentation for production of inulinase using newly isolated Nothophoma anigozanthi JAM
Nisarga Tippanavar, Divya Bhat, Orline Rebello, Girisa Prabhu, Subbalaxmi Selvaraj, Ramananda M. Bhat
Biomass Conversion and Biorefinery.2024; 14(13): 14755. CrossRef
Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production
Sanja Stojanović, Marina Ristović, Jelena Stepanović, Aleksandra Margetić, Bojan Duduk, Zoran Vujčić, Biljana Dojnov
Food Research International.2022; 160: 111755. CrossRef
Solid-state fermentation enhances inulinase and invertase production by Aspergillus brasiliensis
C. Guerrero-Urrutia, T. Volke-Sepulveda, F. Figueroa-Martinez, E. Favela-Torres
Process Biochemistry.2021; 108: 169. CrossRef
Statistical optimization of solid-state fermentation for the production of fungal inulinase from apple pomace
Ram Sarup Singh, Kanika Chauhan, Karminder Kaur, Ashok Pandey
Bioresource Technology Reports.2020; 9: 100364. CrossRef
Optimization of inulinase production by a newly isolated strain Aspergillus flavus var. flavus by solid state fermentation of Saccharum arundinaceum
Deblina Das, Raja Selvaraj, M. Ramananda Bhat
Biocatalysis and Agricultural Biotechnology.2019; 22: 101363. CrossRef
Review of inulinase production using solid-state fermentation
Deblina Das, Ramananda Bhat M, Raja Selvaraj
Annals of Microbiology.2019; 69(3): 201. CrossRef
Immobilized inulinase: a new horizon of paramount importance driving the production of sweetener and prebiotics
Gerard Neeraj, Shobana Ravi, Ravindran Somdutt, ShriAishvarya Kaliyur Ravi, Vaidyanathan Vinoth Kumar
Critical Reviews in Biotechnology.2018; 38(3): 409. CrossRef
The cell wall anchored β-fructosidases of Lactobacillus paracasei : Overproduction, purification, and gene expression control
Petya Velikova, Kaloyan Petrov, Penka Petrova
Process Biochemistry.2017; 52: 53. CrossRef
Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor
Hemant Kumar Rawat, Hemant Soni, Naveen Kango, C. Ganesh Kumar
Biocatalysis and Agricultural Biotechnology.2017; 9: 134. CrossRef
Biotechnological potential of microbial inulinases: Recent perspective
Hemant Kumar Rawat, Hemant Soni, Helen Treichel, Naveen Kango
Critical Reviews in Food Science and Nutrition.2017; 57(18): 3818. CrossRef
Development of heterogeneous preparation with inulinase for tubular reactor systems
M.G. Holyavka, M.P. Evstigneev, V.G. Artyukhov, V.V. Savin
Journal of Molecular Catalysis B: Enzymatic.2016; 129: 1. CrossRef
Recent insights in enzymatic synthesis of fructooligosaccharides from inulin
Ram Sarup Singh, Rupinder Pal Singh, John F. Kennedy
International Journal of Biological Macromolecules.2016; 85: 565. CrossRef
Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides
Hemant Kumar Rawat, Mohd Anis Ganaie, Naveen Kango
Antonie van Leeuwenhoek.2015; 107(3): 799. CrossRef

Purification of carbosymethyl cellulase from hybrid between aspergillus niger and penicillium verruculosum: Yang, Young Ki , Lee, Jung Sup , Park, Hyung Nam , Moon, Myung Nim , Kim, Hong Sub , Kim, Jong Se , Lim, Chae Young , Rhee, Young Ha; J. Microbiol. 1996;34(1):90-94.

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Abstract: The carboxymethyl cellulase (CMCase) was purified from the induced culture filtrate of hybrid TAPW15703 between Aspergillus niger and penicillium verruculosum made by nuclear transfer. The enzyme was purified 80 fold with an overall yield 17% from the culture medium by ammonium sulfate fractionation, Sephadex G-75 gel permeation chromatography, and DEAE-ion exchange column chromatography. The molecular weight of the CMCase has estimated to be 32,000 daltons on SDS-polyacrylamide gel electrophoresis and Sephadex G-150 gel permeation chromatography. The purified enzyme functions optimally at pH 4.0 and 40℃. The Km value for carbosymethyl cellulose was 68 mM. The enzyme activity was increased by the presence of Mg^2+ and Mn^2+.

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