Journal of Microbiology

Observation and Enumeration of Attached Bacteria on Cellulos Film: Ahn, Tae Seok , Choi, Seung Ik , Byeon, Myeong Seop; J. Microbiol. 1995;33(1):1-4.

Abstract: The DNA specific fluorochorome, Hoechst 33258, was used to enumerate the attached bacteria on cellulose film by epifluorescence microscope. Stainning with Hoechst dye gave fine features, whereas staining with acridine orange and DAPI gave poor contrast between background and bacterial cells. In Lake Uiam, Morphologically different two bacteria were detected after 3 days submerging in lake water. In Lake Soyang, the bacterial colonies appeared after 16 hours. This staining method had distinct advantages to enumerate the attached bacteria on surface.

Optimal Protocol for Enumeration of Attached Bacteria on Glass Slides: Hyun Sang Lee , Kae Kyoung Kwon , Jong Ho Lee , Hong Kum Lee; J. Microbiol. 1999;37(4):263-266.

Abstract: In examining bacterial growth on glass surfaces immersed in sea water, we found serious differences between enumeration methods. Therefore, we compared various methods and found sonication and direct count methods were superior to other methods. Since the direct count method was not suitable for long-term investigation, we chose the sonication method and confirmed that sonication periods 8 times for 30 seconds was optimal for the detachment of bacteria from glass surfaces.

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