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- [Minireview] Primary lymphocyte infection models for KSHV and its putative tumorigenesis mechanisms in B cell lymphomas
- Sangmin Kang , Jinjong Myoung
- J. Microbiol. 2017;55(5):319-329. Published online April 29, 2017
- DOI: https://doi.org/10.1007/s12275-017-7075-2
- 55 View
- 0 Download
- 12 Crossref
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Abstract
- Kaposi’s sarcoma-associated herpesvirus (KSHV) is the latest addition to the human herpesvirus family. Unlike alpha- and beta-herpesvirus subfamily members, gamma-herpesviruses, including Epstein-Barr virus (EBV) and KSHV, cause vari-ous tumors in humans. KSHV primarily infects endothelial and B cells in vivo, and is associated with at least three malig-nancies: Kaposi’s sarcoma and two B cell lymphomas, res-pectively. Although KSHV readily infects endothelial cells in vitro and thus its pathogenic mechanisms have been exten-sively studied, B cells had been refractory to KSHV infection. As such, functions of KSHV genes have mostly been eluci-dated in endothelial cells in the context of viral infection but not in B cells. Whether KSHV oncogenes, defined in endo-thelial cells, play the same roles in the tumorigenesis of B cells remains an open question. Only recently, through a few ground-breaking studies, B cell infection models have been established. In this review, those models will be compared and contrasted and putative mechanisms of KSHV-induced B cell transformation will be discussed.
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Citations
Citations to this article as recorded by
- Folate-Targeted Nanocarriers Co-Deliver Ganciclovir and miR-34a-5p for Combined Anti-KSHV Therapy
Fangling Li, Dongdong Cao, Wenyi Gu, Dongmei Li, Zhiyong Liu, Lin Cui
International Journal of Molecular Sciences.2024; 25(5): 2932. CrossRef - Structural aspects of hepatitis E virus
Florencia Cancela, Ofelia Noceti, Juan Arbiza, Santiago Mirazo
Archives of Virology.2022; 167(12): 2457. CrossRef - iTIME.219: An Immortalized KSHV Infected Endothelial Cell Line Inducible by a KSHV-Specific Stimulus to Transition From Latency to Lytic Replication and Infectious Virus Release
Stephen J. Dollery, Tania D. Maldonado, Eric A. Brenner, Edward A. Berger
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Analysis of KSHV B lymphocyte lineage tropism in human tonsil reveals efficient infection of CD138+ plasma cells
Farizeh Aalam, Romina Nabiee, Jesus Ramirez Castano, Jennifer Totonchy, Vera L. Tarakanova
PLOS Pathogens.2020; 16(10): e1008968. CrossRef - Molecular Virology of KSHV in the Lymphocyte Compartment—Insights From Patient Samples and De Novo Infection Models
Farizeh Aalam, Jennifer Totonchy
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef - Molecular and cellular interplay in virus-induced tumors in solid organ recipients
Alessia Gallo, Monica Miele, Ester Badami, Pier Giulio Conaldi
Cellular Immunology.2019; 343: 103770. CrossRef - Middle East respiratory syndrome coronavirus-encoded ORF8b strongly antagonizes IFN-β promoter activation: its implication for vaccine design
Jeong Yoon Lee, Sojung Bae, Jinjong Myoung
Journal of Microbiology.2019; 57(9): 803. CrossRef - Methyltransferase of a cell culture-adapted hepatitis E inhibits the MDA5 receptor signaling pathway
Jinjong Myoung, Jeong Yoon Lee, Kang Sang Min
Journal of Microbiology.2019; 57(12): 1126. CrossRef - Cell Type-Specific Interferon-γ-mediated Antagonism of KSHV Lytic Replication
Mi-Kyung Park, Hyejeong Cho, Seong Woon Roh, Seong-Jun Kim, Jinjong Myoung
Scientific Reports.2019;[Epub] CrossRef - Comment on primary lymphocyte infection models for KSHV and its putative tumorigenesis mechanisms in B cell lymphomas (Journal of Microbiology 2017, 55(5): 319-329)
Giovanna Rappocciolo, Frank Jenkins, Charles R. Rinaldo
Journal of Microbiology.2017; 55(7): 592. CrossRef - Lipids, lipid metabolism and Kaposi’s sarcoma-associated
herpesvirus pathogenesis
Lu Dai, Zhen Lin, Wei Jiang, Erik K. Flemington, Zhiqiang Qin
Virologica Sinica.2017; 32(5): 369. CrossRef - The Roles of Matricellular Proteins in Oncogenic Virus-Induced Cancers and Their Potential Utilities as Therapeutic Targets
Naoyoshi Maeda, Katsumi Maenaka
International Journal of Molecular Sciences.2017; 18(10): 2198. CrossRef
- Folate-Targeted Nanocarriers Co-Deliver Ganciclovir and miR-34a-5p for Combined Anti-KSHV Therapy
Research Support, Non-U.S. Gov't
- KSHV Infection of B-Cell Lymphoma Using a Modified KSHV BAC36 and Coculturing System
- Hyosun Cho , Hyojeung Kang
- J. Microbiol. 2012;50(2):285-292. Published online April 27, 2012
- DOI: https://doi.org/10.1007/s12275-012-1495-9
- 43 View
- 0 Download
- 6 Scopus
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Abstract
- Kaposi’s sarcoma-associated herpesvirus (KSHV) is the causative agent of two B cell lymphoproliferative diseases, namely primary effusion lymphoma (PEL) and multicentric Castleman’s disease (MCD). KSHV infection of B cell lymphoma in vitro has been a long-standing battle in advancing human KSHV biology. In this study, a modified form of KSHV BAC36 named BAC36A significantly increased the fidelity of gene-targeted site-directed mutagenesis in the KSHV genome. This modification eliminates tedious screening steps required to obtain mutant clones when a KSHV BAC36 reverse genetic system is used. Coculturing B-cell lymphoma BJAB cells with KSHV BAC36A stably transfected 293T cells enabled us to infect BJAB cells with a KSHV virion derived from the KSHV BAC36A. The coculture system produced substantial amounts of KSHV infection to BJAB, meaning that KSHV virions were released from 293T cells and then infected neighboring BJAB cells. Owing to our success with the KSHV BAC36A and coculture system, we propose a new genetic system for the study of KSHV gene expression and regulation in B-cell lymphoma.
- Efficient Generation of BLCL Expressing Foreign Antigen as Antigen-presenting Cells with Recombinant Retroviruses
- Hyun-Il Cho , Soon-Young Paik , Il-Hoan Oh , Kyu-Jung Ahn , Dong-Wook Kim , Jin-Han Kang , Wan-Shik Shin , Chun-Choo Kim , Hoon Han
- J. Microbiol. 2001;39(4):300-304.
- 36 View
- 0 Download
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Abstract
- Epstein-Barr Virus (EBV)-transformed lymphoblastoid B cell lines, BLCL, which expresse antigens, are potential antigen-presenting cells (APCs) for the induction of CTL in vitro. However, transfection of BLCLs with subsequent selection by antibiotics is notoriously difficult because plating efficiencies of BLCLs are reported to be 1% or less. To generate stable transfectants of BLCLs, we produced high titers of retroviruses encoding pp65 antigen of human cytomegalovirus as foreign antigens and transduced them to BLCLs. The pp65 gene was cloned into the retroviral vector pLXSN. The recombinant retroviral vector was transfected to ecotropic packaging cell line, GP&E86, and this polyclonal recombinant retrovirus was transduced to PA317 that is amphotropic packaging cell line. The titers of cloned PA317 amphotropic retroviruses ranged from 5 to 7 X 10^6 colony forming units (CFU) per ml (CFU/ml). We performed three rounds of consecutive transductions to BLCLs in order to improve the cloning efficiencies. The expression of recombinant HCMV-pp65 antigen was more than 20% after the final transduction. The third-transduced BLCLs were easily selected in optimal concentration of G418. BLCLs expressing foreign antigens could be used as target cells for CTL assay and/or as APCs for induction of in vitro CTL responses specific for viral and tumor antigens.
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