Journal of Microbiology

Review

Advancements in dengue vaccines: A historical overview and pro-spects for following next-generation candidates: Kai Yan, Lingjing Mao, Jiaming Lan, Zhongdang Xiao; J. Microbiol. 2025;63(2):e2410018. Published online February 27, 2025; DOI: https://doi.org/10.71150/jm.2410018

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Abstract PDF: Dengue, caused by four serotypes of dengue viruses (DENV-1 to DENV-4), is the most prevalent and widely mosquito-borne viral disease affecting humans. Dengue virus (DENV) infection has been reported in over 100 countries, and approximately half of the world's population is now at risk. The paucity of universally licensed DENV vaccines highlights the urgent need to address this public health concern. Action and attention to antibody-dependent enhancement increase the difficulty of vaccine development. With the worsening dengue fever epidemic, Dengvaxia^® (CYD-TDV) and Qdenga^® (TAK-003) have been approved for use in specific populations in affected areas. However, these vaccines do not provide a balanced immune response to all four DENV serotypes and the vaccination cannot cover all populations. There is still a need to develop a safe, broad-spectrum, and effective vaccine to address the increasing number of dengue cases worldwide. This review provides an overview of the existing DENV vaccines, as well as potential candidates for future studies on DENV vaccine development, and discusses the challenges and possible solutions in the field.

Journal Articles

Inhibition of KIF20A suppresses the replication of influenza A virus by inhibiting viral entry: Hoyeon Jeon , Younghyun Lim , In-Gu Lee , Dong-In Kim , Keun Pil Kim , So-Hee Hong , Jeongkyu Kim , Youn-Sang Jung , Young-Jin Seo; J. Microbiol. 2022;60(11):1113-1121. Published online November 1, 2022; DOI: https://doi.org/10.1007/s12275-022-2436-x

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The influenza A virus (IAV) has caused several pandemics, and therefore there are many ongoing efforts to identify novel antiviral therapeutic strategies including vaccines and antiviral drugs. However, influenza viruses continuously undergo antigenic drift and shift, resulting in the emergence of mutated viruses. In turn, this decreases the efficiency of existing vaccines and antiviral drugs to control IAV infection. Therefore, this study sought to identify alternative therapeutic strategies targeting host cell factors rather than viruses to avoid infection by mutated viruses. Particularly, we investigated the role of KIF20A that is one of kinesin superfamily proteins in the replication of IAV. The KIF20A increased viral protein levels in IAV-infected cells by regulating the initial entry stage during viral infection. Furthermore, the KIF20A inhibitor significantly suppressed viral replication, which protected mice from morbidity and mortality. Therefore, our findings demonstrated that KIF20A is highly involved in the viral replication process and viral propagation both in vitro and in vivo, and could thus be used as a target for the development of novel antiviral drugs.

Citations

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Emerging roles of cytoskeletal transport and scaffold systems in human viral propagation
Younghyun Lim, Yong-Bin Cho, Young-Jin Seo
Animal Cells and Systems.2024; 28(1): 506. CrossRef

Anti-inflammatory and anti-oxidative effect of Korean propolis on Helicobacter pylori-induced gastric damage in vitro: Moon-Young Song , Da-Young Lee , Eun-Hee Kim; J. Microbiol. 2020;58(10):878-885. Published online September 2, 2020; DOI: https://doi.org/10.1007/s12275-020-0277-z

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Abstract

Helicobacter pylori, present in the stomach lining, is a Gramnegative bacterium that causes various gastrointestinal diseases, including gastritis and peptic ulcers. Propolis is a natural resinous substance collected from a variety of plants, and contains several natural bioactive substances. The aim of this study was to investigate the anti-inflammatory and antioxidative effects of Korean propolis on H. pylori-induced damage in the human adenocarcinoma gastric cell line. The propolis used in this study was obtained from the Korea Beekeeping Association in South Korea. The expression of pro-inflammatory interleukins (ILs), such as IL-8, IL-12, IL-1β, tumor necrosis factor alpha, cyclooxygenase-2, and inducible nitric oxide synthase, which was increased after H. pylori infection, significantly decreased in a dose-dependent manner upon pretreatment with Korean propolis, because of the suppression of mitogen-activated protein kinases and nuclear factor κB pathway. The anti-oxidative activity of propolis was assessed using the 2,2-diphenyl-1-picrylhydrazyl hydrate free radical assay. Korean propolis showed significant anti-oxidative effects via reactive oxygen species scavenging. In addition, pretreatment with Korean propolis upregulated the expression of anti-oxidant enzymes through Nrf2 signaling activation. These findings indicate that the use of Korean propolis, which has anti-inflammatory and anti-oxidative effects, can be promising for the prevention of H. pylori-induced gastric damage.

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Exploring the Prospective Role of Propolis in Modifying Aging Hallmarks
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Development of a chitosan/propolis-based polymeric system: Characterization, biocompatibility, and modulation of transcription factor expression
Raquel Velázquez-Rodríguez, Amaury Pozos-Guillén, Martha Gabriela Chuc-Gamboa, Juan Valerio Cauich-Rodríguez, Héctor Flores-Reyes, Francisco Javier Tejeda-Nava, Fernando Javier Aguilar-Perez, Diana Maria Escobar Garcia
Journal of Bioactive and Compatible Polymers.2024; 39(5): 329. CrossRef
Therapeutic potential of propolis in alleviating inflammatory response and promoting wound healing in skin burn
Christian Oktavianus Manginstar, Trina Ekawati Tallei, Nurdjannah Jane Niode, Christina Leta Salaki, Sofia Safitri Hessel
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A review for non-antibiotic treatment of Helicobacter pylori: new insight
Neda Shadvar, Sousan Akrami, Seyyed-Mohammad-Amin Mousavi Sagharchi, Rafee Habib Askandar, Alireza Merati, Masoomeh Aghayari, Nikki Kaviani, Hamed Afkhami, Mojtaba Kashfi
Frontiers in Microbiology.2024;[Epub] CrossRef
Wound Healing, Anti-Inflammatory and Anti-Oxidant Activities, and Chemical Composition of Korean Propolis from Different Sources
Aman Dekebo, Chalshisa Geba, Daniel Bisrat, Jin Boo Jeong, Chuleui Jung
International Journal of Molecular Sciences.2024; 25(21): 11352. CrossRef
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Bo Chen, Xinqian Dong, Jinlong Zhang, Wei Wang, Yujiao Song, Xitong Sun, Kangning Zhao, Zhen Sun
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Thach Phan Van, Anh Duy Do
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Jarosław Widelski, Piotr Okińczyc, Katarzyna Suśniak, Anna Malm, Anna Bozhadze, Malkhaz Jokhadze, Izabela Korona-Głowniak
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Therapeutic effect of propolis nanoparticles on wound healing
Juan Yang, Yingjuan He, Sha Nan, Juan Li, Anjuan Pi, Lele Yan, Jinshan Xu, Yuhui Hao
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Recent Update on the Anti-Inflammatory Activities of Propolis
Felix Zulhendri, Ronny Lesmana, Steven Tandean, Andreas Christoper, Kavita Chandrasekaran, Ilham Irsyam, Auliya A. Suwantika, Rizky Abdulah, Nasrul Wathoni
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Construction of a Cuprotosis-Related Gene-Based Model to Improve the Prognostic Evaluation of Patients with Gastric Cancer
Chunyan Han, Kai Zhang, XinKai Mo, Fu Wang
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Honey Bee Products: Preclinical and Clinical Studies of Their Anti-inflammatory and Immunomodulatory Properties
Hesham R. El-Seedi, Nehal Eid, Aida A. Abd El-Wahed, Mostafa E. Rateb, Hanan S. Afifi, Ahmed F. Algethami, Chao Zhao, Yahya Al Naggar, Sultan M. Alsharif, Haroon Elrasheid Tahir, Baojun Xu, Kai Wang, Shaden A. M. Khalifa
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Plant-Based Polyphenols: Anti-Helicobacter pylori Effect and Improvement of Gut Microbiota
María Guerra-Valle, Patricio Orellana-Palma, Guillermo Petzold
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DETERMINATION OF ANTI-UREASE ACTIVITY OF PROPOLIS FROM MARMARA REGION OF TURKEY
Zehra Can, Yakup Kara, Sevgi Kolaylı, İbrahim Çakmak
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Nrf2 Downregulation Contributes to Epithelial-to-Mesenchymal Transition in Helicobacter pylori-Infected Cells
Sarah Bacon, Lornella Seeneevassen, Alison Fratacci, Faustine Rose, Camille Tiffon, Elodie Sifré, Maria M. Haykal, Maya M. Moubarak, Astrid Ducournau, Lucie Bruhl, Stéphane Claverol, Caroline Tokarski, Alina-Roxani Gouloumi, Ioannis S. Pateras, Thomas Dau
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Anti-Inflammatory Effect of Korean Propolis on Helicobacter pylori-Infected Gastric Mucosal Injury Mice Model
Moon-Young Song, Da-Young Lee, Young-Min Han, Eun-Hee Kim
Nutrients.2022; 14(21): 4644. CrossRef
Antifungal Properties of Chemically Defined Propolis from Various Geographical Regions
Marcin Ożarowski, Tomasz M. Karpiński, Rahat Alam, Małgorzata Łochyńska
Microorganisms.2022; 10(2): 364. CrossRef
The Antimicrobial Properties of Poplar and Aspen–Poplar Propolises and Their Active Components against Selected Microorganisms, including Helicobacter pylori
Jarosław Widelski, Piotr Okińczyc, Emil Paluch, Tomasz Mroczek, Jakub Szperlik, Magdalena Żuk, Zbigniew Sroka, Zuriyadda Sakipova, Ioanna Chinou, Krystyna Skalicka-Woźniak, Anna Malm, Izabela Korona-Głowniak
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Research Progress on Therapeutic Effect and Mechanism of Propolis on Wound Healing
Juan Yang, Anjuan Pi, Lele Yan, Juan Li, Sha Nan, Jing Zhang, Yuhui Hao, Juraj Majtan
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"GREEN SYNTHESIS OF NANO-SILVER/SODIUM ALGINATE/CARBOXYMETHYL XANTHAN GUM HYDROGEL AND EVALUATION OF ITS ANTI-INFLAMMATORY AND ANTI-Helicobacter pylori ACTIVITY "
NOURA Y. ELMEHBAD, NADIA A. MOHAMED, NAHED A. ABD EL-GHANY, MARWA M. ABDEL-AZIZ
Cellulose Chemistry and Technology.2022; 56(9-10): 983. CrossRef
Propolis-Loaded Chitosan Nanoparticles for the Treatment of Bacterial Diarrhea: In Vitro and In Vivo Evaluation
Jie Qiao, Jun Yang, Saman Jalili
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Steamed Ginger Extract Exerts Anti-inflammatory Effects in Helicobacter pylori-infected Gastric Epithelial Cells through Inhibition of NF-κB
Moon-Young Song, Da-Young Lee, Sang-Yong Park, Seul-A Seo, Jeong-Seung Hwang, Soo-Hyeon Heo, Eun-Hee Kim
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Short‐term efficacy of a gel containing propolis extract, nanovitamin C and nanovitamin E on peri‐implant mucositis: A double‐blind, randomized, clinical trial
José González‐Serrano, Rosa María López‐Pintor, Julia Serrano, Jesús Torres, Gonzalo Hernández, Mariano Sanz
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Priyanshu Bhargava, Debajit Mahanta, Ashish Kaul, Yoshiyuki Ishida, Keiji Terao, Renu Wadhwa, Sunil C. Kaul
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Simultaneous detection of Salmonella spp., Pseudomonas aeruginosa, Bacillus cereus, and Escherichia coli O157:H7 in environmental water using PMA combined with mPCR: Guoyang Xie , Shuang Yu , Wen Li , Dan Mu , Zoraida P. Aguilar , Hengyi Xu; J. Microbiol. 2020;58(8):668-674. Published online June 25, 2020; DOI: https://doi.org/10.1007/s12275-020-0084-6

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Abstract

A multiplex polymerase chain reaction (mPCR) with propidium monoazide (PMA) and internal amplification control (IAC) for the simultaneous detection of waterborne pathogens Salmonella spp., Pseudomonas aeruginosa, Bacillus cereus, and Escherichia coli O157:H7, was developed. This PMA-IAC-mPCR assay used four new specific primers based on the genes for invA, ecfX, cesB, and fliC, respectively. A 16S rRNA primer was chosen for IAC to eliminate false negative
results
. The photosensitive dye, propidium monoazide (PMA) was used to exclude signals from dead bacteria that could lead to false positive results. In pure culture, the limits of detection (LOD) were 101 CFU/ml for P. aeruginosa, 102 CFU/ml for both Salmonella spp. and E. coli O157:H7, and 103 CFU/ml for B. cereus, respectively. In addition, with a 6–8 h enrichment of all four bacteria that were combined in a mixture that was spiked in water sample matrix, the LOD was 3 CFU/ml for Salmonella spp., 7 CFU/ml for E. coli O157:H7, 10 CFU/ml for B. cereus and 2 CFU/ml for P. aeruginosa. This PMA-IAC-mPCR assay holds potential for application in the multiplex assay of waterborne pathogens.

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Rapid, sensitive, and user-friendly detection of Pseudomonas aeruginosa using the RPA/CRISPR/Cas12a system
Wenjing Zhang, Hai Qu, Xin Wu, Jingjing Shi, Xinling Wang
BMC Infectious Diseases.2024;[Epub] CrossRef
Review of Detection Limits for Various Techniques for Bacterial Detection in Food Samples
Xinyi Zhao, Abhijnan Bhat, Christine O’Connor, James Curtin, Baljit Singh, Furong Tian
Nanomaterials.2024; 14(10): 855. CrossRef
The dual nucleic acid amplification with dynamic light scattering strategy for ultrasensitive detection of Salmonella in milk
Qian Xu, Guoyang Xie, Qiang Shi, Ju Liu, Baoqing Zhou, Ping Tong, Zoraida P. Aguilar, Hengyi Xu
Microchemical Journal.2023; 184: 108143. CrossRef
An Assay Combining Droplet Digital PCR With Propidium Monoazide Treatment for the Accurate Detection of Live Cells of Vibrio vulnificus in Plasma Samples
Ling Hu, Yidong Fu, Shun Zhang, Zhilei Pan, Jiang Xia, Peng Zhu, Jing Guo
Frontiers in Microbiology.2022;[Epub] CrossRef
Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality
Lisa Paruch
International Journal of Environmental Research and Public Health.2022; 19(9): 5128. CrossRef
Rapid-Response Magnetic Enrichment Strategy for Significantly Improving Sensitivity of Multiplex PCR Analysis of Pathogenic Listeria Species
Fangbin Xiao, Xuekun Bai, Keyu Wang, Yifan Sun, Hengyi Xu
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Real-time and visual detection of viableSalmonellain milk by a competitive annealing mediated isothermal amplification (CAMP) combined with propidium monoazide (PMA)
Xu Chen, Wei Li, Yue Ma
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Hybrid RCA-DLS assay combined with aPCR for sensitive Salmonella enteritidis detection
Guoyang Xie, Zhongxu Zhan, Yu Ye, Baoqing Zhou, Ping Tong, Zoraida P. Aguilar, Hengyi Xu
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Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus: Jae Woong Lee , Yu-Jung Won , Lae Hyung Kang , Sung-Geun Lee , Seung-Won Park , Soon-Young Paik; J. Microbiol. 2020;58(8):711-715. Published online May 18, 2020; DOI: https://doi.org/10.1007/s12275-020-0109-1

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Abstract

Severe fever with thrombocytopenia syndrome (SFTS) is being reported annually in South Korea since its first detection there in 2010. The causal agent is a negative-strand RNA virus 80–100 nm in diameter. It causes fever, thrombocytopenia, leukocytopenia, gastrointestinal symptoms, and neural symptoms. The mortality rate of SFTS was 32.6% among 172
case
s reported from 2012 to 2015 in South Korea. Thus, is necessary to develop an effective diagnostic method that selectively identifies the isolates circulating in South Korea. The real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a simple, rapid, and sensitive approach for molecular diagnosis. Here, we designed novel primers for this assay and found that the technique had very high specificity, sensitivity, and efficiency. This real-time RTLAMP approach using the novel primers developed herein can be applied for early diagnosis of SFTSV strains in South Korea to reduce the mortality rate of SFTS.

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Long-Term Detection and Isolation of Severe Fever with Thrombocytopenia Syndrome (SFTS) Virus in Dog Urine
Yumiko Saga, Toshikazu Yoshida, Rieko Yoshida, Shunsuke Yazawa, Takahisa Shimada, Noriko Inasaki, Masae Itamochi, Emiko Yamazaki, Kazunori Oishi, Hideki Tani
Viruses.2023; 15(11): 2228. CrossRef
Advancements in the Worldwide Detection of Severe Fever with Thrombocytopenia Syndrome Virus Infection from 2009 to 2023
Lin Ai, Wei Wang, Zheng Teng
China CDC Weekly.2023; 5(31): 687. CrossRef
Molecular detection of “Candidatus Rickettsia tarasevichiae” by Loop-mediated Isothermal Amplification (LAMP) of the ompA gene
Jing Xue, Qing Ren, Rui Jian, Guang-Cheng Xie, Yongliang Chen, Jiangli Wang, Luanying Du, Wen-Ping Guo
Journal of Microbiological Methods.2022; 202: 106601. CrossRef
Dual-gene detection in a single-tube system based on CRISPR-Cas12a/Cas13a for severe fever thrombocytopenia syndrome virus
Yating Zhu, Chen Xing, Li Yang, Qian Li, Xiaofeng Wang, Jing Zhou, Cong Zhang, Cuiping Ren, Fahu Liu, Jun He, Bing Shen, Yinan Du, Yan Liu
Frontiers in Microbiology.2022;[Epub] CrossRef
CRISPR/Cas12a Technology Combined With RPA for Rapid and Portable SFTSV Detection
Mengqian Huang, Sihua Liu, Yanan Xu, Aqian Li, Wei Wu, Mifang Liang, Guoyu Niu, Zhiyun Wang, Tao Wang
Frontiers in Microbiology.2022;[Epub] CrossRef
Development of monoclonal antibody based IgG and IgM ELISA for diagnosis of severe fever with thrombocytopenia syndrome virus infection
Mei Zhang, Yanhua Du, Li Yang, Lin Zhan, Bin Yang, Xueyong Huang, Bianli Xu, Koichi Morita, Fuxun Yu
The Brazilian Journal of Infectious Diseases.2022; 26(4): 102386. CrossRef
Development of an RT-LAMP Assay for the Rapid Detection of SFTS Virus
Shiori Sano, Shuetsu Fukushi, Souichi Yamada, Shizuko Harada, Hitomi Kinoshita, Satoko Sugimoto, Tomoki Yoshikawa, Takeshi Kurosu, Yuki Takamatsu, Masayuki Shimojima, Shoichi Toda, Yuka Hamada, Naoki Fujisawa, Takayuki Sugimoto, Masayuki Saijo
Viruses.2021; 13(4): 693. CrossRef
A Real-Time Loop-Mediated Isothermal Amplification for Detection of the Wheat Dwarf Virus in Wheat and the Insect VectorPsammotettix alienus
Xingan Hao, Licheng Wang, Xudong Zhang, Qinrong Zhong, Jamal-U-Ddin Hajano, Liangsheng Xu, Yunfeng Wu
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Baseline mapping of severe fever with thrombocytopenia syndrome virology, epidemiology and vaccine research and development
Nathen E. Bopp, Jaclyn A. Kaiser, Ashley E. Strother, Alan D. T. Barrett, David W. C. Beasley, Virginia Benassi, Gregg N. Milligan, Marie-Pierre Preziosi, Lisa M. Reece
npj Vaccines.2020;[Epub] CrossRef

IgG and IgM responses to human papillomavirus L1 virus-like particle as a function of dosing schedule and vaccine formulation: Min-Hye Park , Ji Won You , Hyoung Jin Kim , Hong-Jin Kim; J. Microbiol. 2019;57(9):821-827. Published online August 27, 2019; DOI: https://doi.org/10.1007/s12275-019-9308-z

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Abstract

Most commercialized virus-like particle (VLP) vaccines use aluminum salt as adjuvant, even though VLPs provoke adequate antibody responses without adjuvant. We do not have detailed knowledge of how adjuvant affects the profile of anti- VLP antibodies. Meanwhile, there is evidence that differences between vaccination protocols influence the glycosylation of antibodies, which may alter their effector functions. In the present study a murine model was used to investigate the effects of dosing schedule and adjuvant on the antibody profiles and glycosylation levels of antigen-specific antibody responses to human papillomavirus type 16 L1 (HPV16 L1) VLPs. Mice received subcutaneously 2,000 ng of antigen divided into 4 or 7 doses. The HPV16 L1 VLPs elicited > 4 log10 anti-HPV16 L1 IgG titers without adjuvant, and aluminum hydroxide as adjuvant increased IgG titers 1.3- to 4-fold and reduced the anti-HPV16 L1 IgG2a / anti-HPV16 L1 IgG1 ratio value (use of aluminum hydroxide reduced the ratio of the IgG2a). Immunization with HPV16 L1 VLPs in combination with Freund’s adjuvant enhanced IgG titers 5- to 12- fold. Seven-dose immunization markedly increased anti- HPV16 L1 IgM titers compared to four-dose immunization, as well as increasing the proportion of glycosylated antibodies. Our results suggest that antibody glycosylation can be controlled immunologically, and IgG and IgM profiles and glycosylation profiles of the vaccine-induced antibodies can be used as indicators reflecting the vaccine characteristics. These
results
indicate that the HPV16 L1 VLP dosing schedule can affect the quality of antigen-specific antibody responses. We suggest that dosing schedules should be noted in vaccination protocols for VLP-based vaccines.

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Human papillomavirus vaccines: organisation and experience of preclinical studies
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Chimeric Hepatitis B core virus-like particles harboring SARS-CoV2 epitope elicit a humoral immune response in mice
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Prevalence of antibodies against a cyclic peptide mimicking the FG loop of the human papillomavirus type 16 capsid among Tunisian women
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Roles of eIF4E-binding protein Caf20 in Ste12 translation and P-body formation in yeast: Kiyoung Park , Yu-Seon Lee , Daehee Jung , Jinmi Kim; J. Microbiol. 2018;56(10):744-747. Published online August 22, 2018; DOI: https://doi.org/10.1007/s12275-018-8230-0

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Abstract

Translation initiation factor eIF4E forms eIF4E-eIF4G complex at the 5’ cap of mRNA. This interaction can be inhibited by the family of 4E-binding proteins (4E-BP). In yeast Saccharomyces cerevisiae, two 4E-BPs, Caf20 and Eap1, compete with eIF4G for binding to eIF4E via the shared conserved interaction motif. In order to investigate the roles of Caf20 in gene-specific translational regulation and the formation of mRNA granules (P-bodies), we introduced substitution mutations, caf20-Y4A or caf20-L9A, in the eIF4E-binding motif for CAF20. Overexpression of the wild-type CAF20 showed an increased protein level of Ste12 transcription factor as well as highly developed P-body formation. However, 4E-binding site mutations of CAF20 led to a reduced number of P-body foci and decreased levels of Ste12 protein. The phenotypes of the caf20 deletion mutation were also analyzed, and we suggest that Caf20 plays a critical role in Ste12 protein expression and in the control of P-body formation.

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Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae
Wenyan Cao, Weiquan Zhao, Bolun Yang, Xinning Wang, Yu Shen, Tiandi Wei, Wensheng Qin, Zailu Li, Xiaoming Bao
Microbial Cell Factories.2021;[Epub] CrossRef
Functional association of Loc1 and Puf6 with RNA helicase Dhh1 in translational regulation of Saccharomyces cerevisiae Ste12
Daehee Jung, Jong Seok Seo, Jayoung Nam, Jinmi Kim, Enrico Baruffini
PLOS ONE.2019; 14(7): e0220137. CrossRef

Cecal microbiome divergence of broiler chickens by sex and body weight: Kyu-Chan Lee , Dong Yong Kil , Woo Jun Sul; J. Microbiol. 2017;55(12):939-945. Published online December 7, 2017; DOI: https://doi.org/10.1007/s12275-017-7202-0

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Abstract

The divergence of gut bacterial community on broiler chickens has been reported as potentially possible keys to enhancing nutrient absorption, immune systems, and increasing poultry health and performance. Thus, we compared cecal bacterial communities and functional predictions by sex and body weight regarding the association between cecal microbiota and chicken growth performance. In this study, a total of 12 male and 12 female 1-day-old broiler chickens were raised for 35 days in 2 separate cages. Chickens were divided into 3 subgroups depending on body weight (low, medium, and high) by each sex. We compared chicken cecal microbiota compositions and its predictive functions by sex and body weight difference. We found that bacterial 16S rRNA genes were classified as 3 major phyla (Bacteroidetes, Firmicutes, and Proteobacteria), accounting for > 98% of the total bacterial community. The profiling of different bacterial taxa and predictive metagenome functions derived from 16S rRNA genes were performed over chicken sex and bodyweight. Male chickens were related to the enrichment of Bacteroides while female chickens were to the enrichment of Clostridium and Shigella. Male chickens with high body weight were associated with the enrichment of Faecalibacterium and Shuttleworthia. Carbohydrate and lipid metabolisms were suggested as candidate functions for weight gain in the males. This suggests that the variation of cecal bacterial communities and their functions by sex and body weight may be associated with the differences in the growth potentials of broiler chickens.

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Mutational analysis of the RNA helicase Dhh1 in Ste12 expression and yeast mating: Daehee Jung , Jihye Ahn , Boram Rhee , Jinmi Kim; J. Microbiol. 2017;55(5):373-378. Published online April 29, 2017; DOI: https://doi.org/10.1007/s12275-017-7020-4

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Abstract

Dhh1 and Dhh1 homologues (RCK/p54/DDX6) are mem-bers of the DEAD-box protein family of RNA helicases. These proteins display conserved sequence motifs for ATPase and RNA binding activities. Dhh1 is a component of the P-bodies (processing bodies) of mRNA granules and functions as an mRNA decapping activator in Saccharomyces cerevisiae. Dhh1 also contributes to gene-specific regulation during yeast mating. The dhh1 deletion mutation results in a significant decrease in the expression of Ste12, a mating-specific trans-cription factor, showing severe mating defects. Here, we in-troduced amino-acid substitution mutations in the ATPase and RNA binding domains of Dhh1 and also constructed a deletion of 79 amino acids at the Q/P-rich C-terminal region. The mutations in ATPase A and B motif (K96R, D195A) and C-terminus deletion showed reduced levels of mating effi-ciency as well as Ste12 protein expression. The Q/P-rich C- terminal region of Dhh1 was dispensable for growth at non- permissive temperature 37°C but appeared to play an im-portant role in regulating the Ste12 protein expression and mating processes. The P-body accumulation induced by treatment with α-mating factor required ATPase, RNA-bind-ing and the Q/P-rich C-terminal domains of Dhh1.

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Fus3 and Tpk2 protein kinases regulate the phosphorylation-dependent functions of RNA helicase Dhh1 in yeast mating and Ste12 protein expression
Jaehee Hwang, Daehee Jung, Jinmi Kim
Journal of Microbiology.2022; 60(8): 843. CrossRef
The Role of DEAD-Box ATPases in Gene Expression and the Regulation of RNA–Protein Condensates
Karsten Weis, Maria Hondele
Annual Review of Biochemistry.2022; 91(1): 197. CrossRef
Roles of Dhh1 RNA helicase in yeast filamentous growth: Analysis of N-terminal phosphorylation residues and ATPase domains
Eunji Lee, Daehee Jung, Jinmi Kim
Journal of Microbiology.2020; 58(10): 853. CrossRef
Functional association of Loc1 and Puf6 with RNA helicase Dhh1 in translational regulation of Saccharomyces cerevisiae Ste12
Daehee Jung, Jong Seok Seo, Jayoung Nam, Jinmi Kim, Enrico Baruffini
PLOS ONE.2019; 14(7): e0220137. CrossRef
Roles of eIF4E-binding protein Caf20 in Ste12 translation and P-body formation in yeast
Kiyoung Park, Yu-Seon Lee, Daehee Jung, Jinmi Kim
Journal of Microbiology.2018; 56(10): 744. CrossRef

Research Support, Non-U.S. Gov't

Immunological charaterization of monoclonal antibodies used in rapid influenza diagnostic test for detection of the 2009 pandemic influenza A(H1N1)pdm09 infection: Hwajung Yi , Mi-Seon Lee , Joo-Yeon Lee , Hae Kyung Lee , Chun Kang; J. Microbiol. 2015;53(2):166-175. Published online January 28, 2015; DOI: https://doi.org/10.1007/s12275-015-4642-2

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Abstract

Since the 2009 pandemic, monoclonal antibodies (mAbs) for rapid influenza diagnostic tests (RIDT) have been developed for specific diagnostics of pandemic viral infection. Most of the mAbs were poorly characterized because of urgency during the pandemic. Further characterization of the mAbs for RIDTs would be beneficial for understanding the immunological properties of the pandemic virus and utilizing the mAbs for other research purposes. In this study, it was confirmed that two mAbs (I38 and D383) in an RIDT for H1N1pdm09 diagnostics were able to detect H1N1pdm09 virus through enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay (IFA). Also, the two mAbs exhibited reactivity to hemagglutinins (HAs) of both the H1N1pdm09 and 1918 H1N1 viruses; therefore, the RIDT using the mAbs could detect HAs of H1N1pdm09 and also HAs of 1918 H1N1-like strains. In an extension to our previous study, the epitopes (Sa antigenic site and the interface area of F?and vestigial esterase subdomains on the HA1 domain of HA of H1N1pdm09) recognized by the mAbs were corroborated in depth by IFA with escape-mutants from the mAbs and mapping of the epitopes on the crystal structure of human H1N1 viral HAs. Collectively, these results imply that the mAbs for the RIDT may be suitable for use in studying the immunological properties of H1N1pdm09 viruses and that the Sa antigenic site and the interface area between F?and vestigial esterase subdomains on influenza viral HA recognized by the mAbs are immunologically conserved regions between H1N1pdm09 and 1918 H1N1.

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Antigenic diversity of H5 highly pathogenic avian influenza viruses of clade 2.3.4.4 isolated in Asia
Ayako Ohkawara, Masatoshi Okamatsu, Makoto Ozawa, Duc‐Huy Chu, Lam Thanh Nguyen, Takahiro Hiono, Keita Matsuno, Hiroshi Kida, Yoshihiro Sakoda
Microbiology and Immunology.2017; 61(5): 149. CrossRef

Journal Article

Anti protein A antibody-gold nanorods conjugate: a targeting agent for selective killing of methicillin resistant Staphylococcus aureus using photothermal therapy method: Rasoul Shokri , Mojtaba Salouti , Rahim Sorouri Zanjani; J. Microbiol. 2015;53(2):116-121. Published online January 28, 2015; DOI: https://doi.org/10.1007/s12275-015-4519-4

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Abstract

The high prevalence of methicillin resistant Staphylococcus aureus (MRSA) and developing resistance to antibiotics requires new approaches for treatment of infectious diseases due to this bacterium. In this study, we developed a targeting agent for selective killing of MRSA using photothermal therapy method based on anti protein A antibody and gold nanorods (GNRs). Polystyrene sulfonate (PSS) coated GNRs were conjugated with anti protein A antibody. The FT-IR and UV-vis analyses approved the formation of anti protein A antibody-gold nanorods conjugate. In vitro study of photothermal therapy showed 82% reduction in the MRSA cells viability which was significantly greater than the ablation effect of free GNRs and laser alone. Significant accumulation of anti protein A antibody-GNRs in the infected muscle in comparison with normal muscle approved the targeting ability of new agent. In vivo study of photothermal therapy resulted in a significant reduction (73%) in the bacterial cells viability in the infected mouse model. These results demonstrated the ability of anti protein A antibody-GNRs conjugate in combination with NIR laser energy for selective killing of MRSA in mouse model.

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Validation Study

Comparison of JEV Neutralization Assay Using Pseudotyped JEV with the Conventional Plaque-Reduction Neutralization Test: Hee-Jung Lee , Kyung-Il Min , Ki Hoon Park , Hyo Jung Choi , Min-Kyoung Kim , Chi-Young Ahn , Young-Jin Hong , Young Bong Kim; J. Microbiol. 2014;52(5):435-440. Published online March 7, 2014; DOI: https://doi.org/10.1007/s12275-014-3529-y

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Abstract

We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV- PV). JEV-PV-based neutralization assay offers several advan-tages compared with the current standard plaque-reduc-tion neutralization test (PRNT), including simplicity, safety, and speed. To evaluate the suitability of the JEV-PV assay as new replacement neutralization assay, we compared its repeatability, reproducibility, specificity, and correlated its results with those obtained using the PRNT. These analyses showed a close correlation between the results obtained with the JEV-PV assay and the PRNT, using the 50% plaque re-duction method as a standard for measuring NAb titers to JEV. The validation results met all analytical acceptance criteria. These results suggest that the JEV-PV assay could serve as a safe and simple method for measuring NAb titer against JEV and could be used as an alternative approach for assaying the potency of JEV neutralization.

Citations

Citations to this article as recorded by

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Research Support, Non-U.S. Gov't

Neutralization Potential of the Plasma of HIV-1 Infected Indian Patients in the Context of Anti-V3 Antibody Content and Antiretroviral Theraphy: Alok Kumar Choudhary , Raiees Andrabi , Somi Sankaran Prakash , Rajesh Kumar , Shubhasree Dutta Choudhury Choudhury , Naveet Wig , Ashutosh Biswas , Anjali Hazarika , Kalpana Luthra; J. Microbiol. 2012;50(1):149-154. Published online February 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1246-y

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Abstract: We assessed the anti-V3 antibody content and viral neutralization potential of the plasma of 63 HIV-1-infected patients (antiretroviral naïve=39, treated=24) against four primary isolates (PIs) of clade C and a tier 1 clade B isolate SF162. Depletion and inhibition of anti-V3 antibodies in the plasma of five patients with high titers of anti-V3 antibodies led to modest change in the neutralization percentage against two PIs (range 0–21%). The plasma of antiretroviral-treated patients exhibited higher neutralization potential than that of the drug-naïve plasmas against the four PIs tested which was further evidenced by a follow-up study.

Journal Article

NOTE] Sawadaea koelreuteriae comb. nov., a Powdery Mildew of Koelreuteria paniculata: Hyeon-Dong Shin , Mi-Jeong Park; J. Microbiol. 2011;49(5):862-866. Published online November 9, 2011; DOI: https://doi.org/10.1007/s12275-011-1479-1

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6 Scopus

Abstract: A powdery mildew parasitizing Koelreuteria spp. was first described under the name Uncinula koelreuteriae Miyake and later transferred to the genus Typhulochaeta. Based on morphological and molecular data of several herbarium specimens collected from Korea, the generic placement of Typhulochaeta is discussed and T. koelreuteriae is combined in the genus Sawadaea. Redescription and epitypification of this species is provided hereby.

Research Support, Non-U.S. Gov't

Fine Mapping of a Foot-and-Mouth Disease Virus Epitope Recognized by Serotype-Independent Monoclonal Antibody 4B2: Yongzhong Yu , Haiwei Wang , Lei Zhao , Chunyuan Zhang , Zhigang Jiang , Li Yu; J. Microbiol. 2011;49(1):94-101. Published online March 3, 2011; DOI: https://doi.org/10.1007/s12275-011-0134-1

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26 Scopus

Abstract: VP2 is a structural protein of the foot-and-mouth disease virus (FMDV). In this study, a FMDV serotype-independent monoclonal antibody (MAb), 4B2, was generated. By screening a phage-displayed random 12-peptide library, we found positive phages displaying the consensus motif ETTXLE (X is any amino acid (aa)), which is highly homologous to 6ETTLLE11 at the N-terminus of the VP2 protein. Subsequently, a series of GST-fusion proteins expressing a truncated N-terminus of VP2 were examined by western blot analysis using the MAb 4B2. The results indicated that the motif 6ETTLLE11 of VP2 may be the minimal requirement of the epitope recognized by 4B2. Moreover, a 12-aa peptide 2KKTEETTLLEDR13 was shown to be the minimal unit of the epitope with maximal binding activity to 4B2. Alanine-scanning analysis demonstrated thatThr7, Thr8, and Leu10 are the functional residues of the 4B2 epitope Glu6 and Leu9 are required residues, and Glu11 plays a crucial role in the binding of MAb 4B2. The fine mapping of the epitope indicated that MAb 4B2 has the potential to be used in FMDV diagnosis.

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