Journal of Microbiology

Journal Article

Lipoteichoic Acid from Lacticaseibacillus rhamnosus GG as a Novel Intracanal Medicament Targeting Enterococcus faecalis Biofilm Formation: Ji-Young Yoon, Somin Park, Dongwook Lee, Ok-Jin Park, WooCheol Lee, Seung Hyun Han; J. Microbiol. 2024;62(10):897-905. Published online September 30, 2024; DOI: https://doi.org/10.1007/s12275-024-00165-6

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Abstract: The demand for safe and effective endodontic medicaments to control Enterococcus faecalis biofilms, a contributor to apical periodontitis, is increasing. Recently, lipoteichoic acid (LTA) of family Lactobacillaceae has been shown to have anti-biofilm effects against various oral pathogens. Preliminary experiments showed that LTA purified from Lacticaseibacillus rhamnosus GG (Lgg.LTA) was the most effective against E. faecalis biofilms among LTAs from three Lactobacillaceae including L. rhamnosus GG, Lacticaseibacillus casei, and Lactobacillus acidophilus. Therefore, in this study, we investigated the potential of Lgg.LTA as an intracanal medicament in human root canals infected with E. faecalis. Twenty eight dentinal cylinders were prepared from extracted human teeth, where two-week-old E. faecalis biofilms were formed followed by intracanal treatment with sterile distilled water (SDW), N-2 methyl pyrrolidone (NMP), calcium hydroxide (CH), or Lgg.LTA. Bacteria and biofilms that formed in the root canals were analyzed by scanning electron microscopy and confocal laser scanning microscopy. The remaining E. faecalis cells in the root canals after intracanal medicament treatment were enumerated by culturing and counting. When applied to intracanal biofilms, Lgg.LTA effectively inhibited E. faecalis biofilm formation as much as CH, while SDW and NMP had little effect. Furthermore, Lgg.LTA reduced both live and dead bacteria within the dentinal tubules, indicating the possibility of minimal re-infection in the root canals. Collectively, intracanal application of Lgg.LTA effectively inhibited E. faecalis biofilm formation, implying that Lgg.LTA can be used as a novel endodontic medicament.

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Paenibacillus swuensis sp. nov., a Bacterium Isolated from Soil: Jae-Jin Lee , Da-Hye Yang , Ye-Sil Ko , Jae-Kyoung Park , Eun-Young Im , Ju-Yeon Kim , Ka-Young Kwon , Yu-Jung Lee , Hyung-Mi Kim , Myung Kyum Kim; J. Microbiol. 2014;52(2):106-110. Published online February 1, 2014; DOI: https://doi.org/10.1007/s12275-014-3546-x

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Strain DY6T, a Gram-positive endospore-forming motile rodshaped bacterium, was isolated from soil in South Korea and characterized to determine its taxonomic position. Phylogenetic analyses based on the 16S rRNA gene sequence of strain DY6T revealed that strain DY6T belongs to the genus Paenibacillus in the family Paenibacillaceae in the class Bacilli. The highest degree of sequence similarities of strain DY6T were found with Paenibacillus gansuensis B518T (97.9%), P. chitinolyticus IFO 15660T (95.3%), P. chinjuensis WN9T (94.7%), and P. rigui WPCB173T (94.7%). Chemotaxonomic data revealed that the predominant fatty acids were anteiso-C15:0 (38.7%) and C16:0 (18.0%). A complex polar lipid profile consisted of major amounts of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The predominant respiratory quinone was MK-7. Based on these phylogenetic, chemotaxonomic, and phenotypic data, strain DY6T (=KCTC 33026T =JCM 18491T) should be classified as a type strain of a novel species, for which the name Paenibacillus swuensis sp. nov. is proposed.

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Paenibacillus arcticus sp. nov., isolated from Arctic soil
In-Tae Cha, Eui-Sang Cho, Yesol Yoo, Yoon Ji Seok, Inhye Park, Hee Seon Lim, Jung-Min Park, Seong Woon Roh, Young-Do Nam, Hak-Jong Choi, Yoo Kyung Lee, Myung-Ji Seo
International Journal of Systematic and Evolutionary Microbiology.2017; 67(11): 4385. CrossRef
Paenibacillus albidus sp. nov., isolated from grassland soil
Junli Zhuang, Di Xin, Yu-Qin Zhang, Jingnan Guo, Jianli Zhang
International Journal of Systematic and Evolutionary Microbiology.2017; 67(11): 4685. CrossRef
Complete genome sequence of Paenibacillus swuensis DY6T, a bacterium isolated from gamma-ray irradiated soil
Myung Kyum Kim, Seung-Yeol Lee, Hee-Young Jung, Sathiyaraj Srinivasan
The Korean Journal of Microbiology.2016; 52(4): 500. CrossRef
Paenibacillus baekrokdamisoli sp. nov., isolated from soil of crater lake
Keun Chul Lee, Kwang Kyu Kim, Jong-Shik Kim, Dae-Shin Kim, Suk-Hyung Ko, Seung-Hoon Yang, Jung-Sook Lee
International Journal of Systematic and Evolutionary Microbiology.2016; 66(5): 1937. CrossRef
List of new names and new combinations previously effectively, but not validly, published
A. Oren, G. M. Garrity
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY.2015; 65(Pt 3): 741. CrossRef

Bacillus kyonggiensis sp. nov., Isolated from Soil of a Lettuce Field: Ke Dong , Sangseob Lee; J. Microbiol. 2011;49(5):776-781. Published online November 9, 2011; DOI: https://doi.org/10.1007/s12275-011-1218-7

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Abstract

A Gram-positive, rod-shaped, motile, endospore-forming bacterial strain, designated NB22T, was isolated from soil of a lettuce field in Kyonggi province, South Korea, and was characterized by using a polyphasic taxonomic approach. This novel isolate grew optimally at 30-37°C and pH 8-9. It grew in the presence of 0-4% NaCl (optimum, 1-2%). Comparative 16S rRNA gene sequence analysis showed that strain NB22T was closely related to members of the genus Bacillus and fell within a coherent cluster comprising B. siralis 171544T (98.1%) and B. korlensis ZLC-26T (97.3%). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species with validly published names were less than 96.4%. Strain NB22T had a genomic DNA G+C content of 36.3 mol% and the predominant respiratory quinone was MK-7. The peptidoglycan contained meso-diaminopimelic acid. The major cellular fatty acids were iso-C15:0, anteiso-C15:0, C14:0, and C16:0. These chemotaxonomic results supported the affiliation of strain NB22T to the genus Bacillus, and the low DNA-DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain NB22T from recognized Bacillus species. On the basis of the evidence presented, strain NB22T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus kyonggiensis sp. nov. is proposed. The type strain is NB22T (=KEMB 5401-267T =JCM 17569T).

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Comparative genomic analyses of the genus Robertmurraya and proposal of the novel species Robertmurraya mangrovi sp. nov., isolated from mangrove soil
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Bacillus yapensis sp. nov., a novel piezotolerant bacterium isolated from deep-sea sediment of the Yap Trench, Pacific Ocean
Xiashutong Xu, Libo Yu, Guangxin Xu, Qilin Wang, Shiping Wei, Xixiang Tang
Antonie van Leeuwenhoek.2020; 113(3): 389. CrossRef
Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it o
Radhey S. Gupta, Sudip Patel, Navneet Saini, Shu Chen
International Journal of Systematic and Evolutionary Microbiology .2020; 70(11): 5753. CrossRef
Production of Biosurfactant Produced from Used Cooking Oil by Bacillus sp. HIP3 for Heavy Metals Removal
Nurul Hanisah Md Badrul Hisham, Mohamad Faizal Ibrahim, Norhayati Ramli, Suraini Abd-Aziz
Molecules.2019; 24(14): 2617. CrossRef
Tumebacillus luteolus sp. nov., isolated from soil
Jihee Her, Sathiyaraj Srinivasan, Sang-Seob Lee
International Journal of Systematic and Evolutionary Microbiology .2015; 65(Pt_11): 4107. CrossRef

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