Journal of Microbiology

Journal Article

Identification of a novel phospholipase D gene and effects of carbon sources on its expression in Bacillus cereus ZY12: Yu Zhao , Yinfeng Xu , Fang Yu , Chunzhi Zhang; J. Microbiol. 2018;56(4):264-271. Published online April 2, 2018; DOI: https://doi.org/10.1007/s12275-018-7529-1

49 View
0 Download
3 Crossref

In the present study, a new strain, Bacillus cereus ZY12, producing phospholipase D (PLD) was identified. The expression of PLD in this strain was found to be induced by its substrate, phosphatidylcholine (PC), and completely silenced by other carbon sources, such as glucose, fructose, and maltose, which are generally used in microbial growth cultures, thus presenting a unique expression pattern different from other PLD-producing microorganisms. This study is the first to report on the ability of B. cereus to produce PLD, and successfully clone its PLD-coding gene and identify its function, extending the knowledge on PLD distribution and evolution in microorganisms.

Citations

Citations to this article as recorded by

Structural insights into PA3488-mediated inactivation of Pseudomonas aeruginosa PldA
Xiaoyun Yang, Zongqiang Li, Liang Zhao, Zhun She, Zengqiang Gao, Sen-Fang Sui, Yuhui Dong, Yanhua Li
Nature Communications.2022;[Epub] CrossRef
Construction of a Super-Folder Fluorescent Protein-Guided Secretory Expression System for the Production of Phospholipase D in Bacillus subtilis
Haiyang Zhang, Xuehan Li, Qi Liu, Jianan Sun, Francesco Secundo, Xiangzhao Mao
Journal of Agricultural and Food Chemistry.2021; 69(24): 6842. CrossRef
Microbial phospholipase D: Identification, modification and application
Zhenxia Zhang, Ming Chen, Wei Xu, Wenli Zhang, Tao Zhang, Cuie Guang, Wanmeng Mu
Trends in Food Science & Technology.2020; 96: 145. CrossRef

Research Support, Non-U.S. Gov'ts

NOTE] Bacillus manliponensis sp. nov., a New Member of the Bacillus cereus Group Isolated from Foreshore Tidal Flat Sediment: Min Young Jung , Joong-Su Kim , Woon Kee Paek , Jeongheui Lim , Hansoo Lee , Pyoung Il Kim , Jin Yeul Ma , Wonyong Kim , Young-Hyo Chang; J. Microbiol. 2011;49(6):1027-1032. Published online December 28, 2011; DOI: https://doi.org/10.1007/s12275-011-1049-6

26 View
0 Download
53 Scopus

Abstract: A Gram-positive, endospore-forming, new Bacillus species, strain BL4-6T, was isolated from tidal flat sediment of the Yellow Sea. Strain BL4-6T is a straight rod, with motility by peritrichate flagella. The cell wall contains meso-diaminopimelic acid, and the major respiratory quinone is menaquinone-7. The major fatty acids are iso-C15:0 and summed feature 3 (containing C16:1 ω7c/ iso-C15:0 2OH, and/or iso-C15:0 2OH/C16:1 ω7c). Cells are catalase-positive and oxidase-negative. The G+C content of the genomic DNA is 38.0 mol%. Based on a comparative 16S rRNA gene sequence analysis, the isolate belongs to the genus Bacillus, forms a clade with the Bacillus cereus group, and is closely related to Bacillus mycoides (98.5%), Bacillus cereus (98.5%), Bacillus anthracis (98.4%), Bacillus thuringiensis (98.4%), Bacillus weihenstephanensis (98.1%), and Bacillus pseudomycoides (97.5%). The isolate showed less than 85% similarity of the gyrA gene sequence and below 95% similarity of the rpoB gene sequence to the members of this group. DNA-DNA relatedness between strain BL4-6T and B. cereus group was found to be in a range of 22.8-42.3%, and thus BL4-6T represents a unique species. On the basis of these studies, strain BL4-6T (=KCTC 13319T =JCM 15802T) is proposed to represent the type strain of a novel species, Bacillus manliponensis sp. nov.

Characterization of Calcium-Activated Bifunctional Peptidase of the Psychrotrophic Bacillus cereus: Jong-Il Kim , Sun-Min Lee , Hyun-Joo Jung; J. Microbiol. 2005;43(3):237-243.; DOI: https://doi.org/2219 [pii]

39 View
0 Download

Abstract: The protease purified from Bacillus cereus JH108 has the function of leucine specific endopeptidase. When measured by hydrolysis of synthetic substrate (N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide), the enzyme activity exhibited optimal activity at pH 9.0, 60^oC. The endopeptidase activity was stimulated by Ca^+^+, Co^+^+, Mn^+^+, Mg^+^+, and Ni^+^+, and was inhibited by metal chelating agents such as EDTA, 1,10-phenanthroline, and EGTA. Addition of serine protease inhibitor, PMSF, resulted in the elimination of the activity. The endopeptidase activity was fully recovered from the inhibition of EDTA by the addition of 1 mM Ca^+^+, and was partially restored by Co^+^+ and Mn^+^+, indicating that the enzyme was stabilized and activated by divalent cations and has a serine residue at the active site. Addition of Ca^+^+ increased the pH and heat stability of endopeptidase activity. These results show that endopeptidase requires calcium ions for activity and/or stability. A Lineweaver-Burk plot analysis indicated that the K_m value of endopeptidase is 0.315 mM and V_max is 0.222 mmol of N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide per min. Bestatin was shown to act as a competitive inhibitor to the endopeptidase activity.

Editorial

[SPECIAL ISSUE]Two years of COVID-19 pandemic: where are we now?: Jinjong Myoung; J. Microbiol. 2022;60(3):235-237.; DOI: https://doi.org/10.1007/s12275-022-1679-x

58 View
0 Download
15 Web of Science
12 Crossref

Citations

Citations to this article as recorded by

Real-time infectious disease endurance indicator system for scientific decisions using machine learning and rapid data processing
Shivendra Dubey, Dinesh Kumar Verma, Mahesh Kumar
PeerJ Computer Science.2024; 10: e2062. CrossRef
Autoimmune/inflammatory syndrome induced by adjuvants (ASIA): past, present, and future implications
Isa Seida, Mahmoud Alrais, Ravend Seida, Abdulkarim Alwani, Zeynep Kiyak, Abdulrahman Elsalti, Sevval Nil Esirgun, Tunahan Abali, Naim Mahroum
Clinical and Experimental Immunology.2023; 213(1): 87. CrossRef
Surgical Treatment of Another Sequalae of COVID-19: Post-COVID CTEPH
Nezih Onur Ermerak, Sehnaz Olgun Yildizeli, Derya Kocakaya, Bulent Mutlu, Koray Ak, Serpil Tas, Bedrettin Yildizeli
The Thoracic and Cardiovascular Surgeon.2023; 71(05): 413. CrossRef
Distinctive Combinations of RBD Mutations Contribute to Antibody Evasion in the Case of the SARS-CoV-2 Beta Variant
Tae-Hun Kim, Sojung Bae, Sunggeun Goo, Jinjong Myoung
Journal of Microbiology and Biotechnology.2023; 33(12): 1587. CrossRef
Paxlovid for Treating COVID-19 Patients: A Case-Control Study From Two Hospitals in the Eastern Province of Saudi Arabia
Ali Alsaeed, Abdullah Alkhalaf, Ali Alomran, Walaa Alsfyani, Fadhel Alhaddad, Mousa J Alhaddad
Cureus.2023;[Epub] CrossRef
Ethical and coordinative challenges in setting up a national cohort study during the COVID-19 pandemic in Germany
Katharina Tilch, Sina M. Hopff, Katharina Appel, Monika Kraus, Bettina Lorenz-Depiereux, Lisa Pilgram, Gabi Anton, Sarah Berger, Ramsia Geisler, Kirsten Haas, Thomas Illig, Dagmar Krefting, Roberto Lorbeer, Lazar Mitrov, Maximilian Muenchhoff, Matthias Na
BMC Medical Ethics.2023;[Epub] CrossRef
Impact of the COVID-19 pandemic on the conduct of non-COVID-19 clinical trials: protocol for a scoping review
Wenru Shang, Lili Wei, Yujia Liu, Haosheng Pu, Xiuxia Li, Junqiang Niu, Long Ge, Cuncun Lu, Kehu Yang
BMJ Open.2023; 13(10): e074128. CrossRef
Amiodarone for the Treatment of Arrhythmias in COVID-19 Patients Does Not Increase the Risk of Pulmonary Fibrosis: A Retrospective Cohort Study
David B Money, Dae Hyun Lee, Ari Hadar, Justin Doherty, Christopher Malanga, Alexa Serino, Adam J Cohen
Cureus.2023;[Epub] CrossRef
Loyalty in the time of COVID-19: A review of the literature in tourism destination settings
Oliver Cruz-Milán
Frontiers in Psychology.2023;[Epub] CrossRef
Challenges of rapamycin repurposing as a potential therapeutic candidate for COVID-19: implications for skeletal muscle metabolic health in older persons
Matthew J. Lees, Nathan Hodson, Cassidy T. Tinline-Goodfellow, Hugo J. W. Fung, Antonis Elia, Daniel R. Moore
American Journal of Physiology-Endocrinology and Metabolism.2022; 322(6): E551. CrossRef
Use of Alcohol, Cannabinoids, Psychostimulants, and Sedatives before and during the COVID-19 Pandemic among Students in 40 European Countries
Mateusz Babicki
International Journal of Environmental Research and Public Health.2022; 19(22): 14879. CrossRef
Evaluation of Vaccine Hesitancy and Anxiety Levels among Hospital Cleaning Staff and Caregivers during COVID-19 Pandemic
Sami Akbulut, Ayse Gokce, Gulseda Boz, Hasan Saritas, Selver Unsal, Ali Ozer, Mehmet Serdar Akbulut, Cemil Colak
Vaccines.2022; 10(9): 1426. CrossRef

Characterization of Bacillus cereus SH-7 Extracellular Protease: Hak Kyu Yi , Young Jin Chum , Han Bok Kim; J. Microbiol. 1999;37(4):213-217.

40 View
0 Download

Abstract: An extracellular endopeptidase from Bacillus cereus SH-7 was purified to homogeneity. The protease was most active at pH 8 and 40 C, respectively. The molecular mass of the protease was 40 kDa on SDS-PAGE, and 120 kDa by gel filtration, suggesting that the native enzyme is composed of three homogeneous subunits. The K_m and V_max values of the protease for N-succinyl-(Ala)_2-Pro-Phe-p-nitroanilide were 11.11 mM and 170 nmol/mg of protein/min, respectively. The protease was also identified as a metalloprotease. The bioactivity of the SH-7 protease will need further study in the future.

First
Prev
Page of 1
Next
Last

Search