Journal Article
- Non-ureolytic calcium carbonate precipitation by Lysinibacillus sp. YS11 isolated from the rhizosphere of Miscanthus sacchariflorus
-
Yun Suk Lee , Hyun Jung Kim , Woojun Park
-
J. Microbiol. 2017;55(6):440-447. Published online May 28, 2017
-
DOI: https://doi.org/10.1007/s12275-017-7086-z
-
-
49
View
-
0
Download
-
37
Crossref
-
Abstract
-
Although microbially induced calcium carbonate precipita-tion (MICP) through ureolysis has been widely studied in en-vironmental engineering fields, urea utilization might cause environmental problems as a result of ammonia and nitrate production. In this study, many non-ureolytic calcium car-bonate-precipitating bacteria that induced an alkaline envi-ronment were isolated from the rhizosphere of Miscanthus sacchariflorus near an artificial stream and their ability to pre-cipitate calcium carbonate minerals with the absence of urea was investigated. MICP was observed using a phase-contrast microscope and ion-selective electrode. Only Lysinibacillus sp. YS11 showed MICP in aerobic conditions. Energy disper-sive X-ray spectrometry and X-ray diffraction confirmed the presence of calcium carbonate. Field emission scanning elec-tron microscopy analysis indicated the formation of morpho-logically distinct minerals around cells under these condi-tions. Monitoring of bacterial growth, pH changes, and Ca2+ concentrations under aerobic, hypoxia, and anaerobic con-ditions suggested that strain YS11 could induce alkaline con-ditions up to a pH of 8.9 and utilize 95% of free Ca2+ only under aerobic conditions. Unusual Ca2+ binding and its re-lease from cells were observed under hypoxia conditions. Bio-film and extracellular polymeric substances (EPS) formation were enhanced during MICP. Strain YS11 has resistance at high pH and in high salt concentrations, as well as its spore- forming ability, which supports its potential application for self-healing concrete.
-
Citations
Citations to this article as recorded by

- Revolutionizing remediation: Unveiling the power of Lysinibacillus sp. in tackling heavy metal stress
Akanksha Gupta, Chhavi Siwach, Virendra Kumar Mishra
Sustainable Chemistry for the Environment.2024; 8: 100156. CrossRef - Overcoming the inhibitory effects of urea to improve the kinetics of microbial-induced calcium carbonate precipitation (MICCP) by Lysinibacillus sphaericus strain MB284
Seyed Ali Rahmaninezhad, Mohammad Houshmand, Amirreza Sadighi, Kiana Ahmari, Divya Kamireddi, Reva M. Street, Yaghoob (Amir) Farnam, Caroline L. Schauer, Ahmad Raeisi Najafi, Christopher M. Sales
Journal of Bioscience and Bioengineering.2024; 138(1): 63. CrossRef - Comparison of calcium carbonate production by bacterial isolates from recycled aggregates
Giuseppe Ciaramella Moita, Vitor da Silva Liduino, Eliana Flávia Camporese Sérvulo, João Paulo Bassin, Romildo Dias Toledo Filho
Environmental Science and Pollution Research.2024; 31(25): 37810. CrossRef - Microbially Induced Calcium Carbonate Precipitation Using Lysinibacillus sp.: A Ureolytic Bacterium from Uttarakhand for Soil Stabilization
Aparna Ganapathy Vilasam Sreekala, Sreelakshmi Nair, Vinod Kumar Nathan
Current Microbiology.2024;[Epub] CrossRef - Application of Microbially Induced CaCO3 on the Reinforcement of Rock Discontinuity
Simiao Zhang, Shuhong Wang, Zulkifl Ahmed, Fahad Alshawmar
Applied Sciences.2024; 14(19): 8952. CrossRef - Exploring a cellulose-immobilized bacteria for self-healing concrete via microbe-induced calcium carbonate precipitation
Jian-Miao Xu, Zhuo-Ting Chen, Feng Cheng, Zhi-Qiang Liu, Yu-Guo Zheng
Journal of Building Engineering.2024; 95: 110248. CrossRef - Trends and opportunities for greener and more efficient microbially induced calcite precipitation pathways: a strategic review
Kishan Bhadiyadra, Siaw Chian Jong, Dominic E L Ong, Jeung-Hwan Doh
Geotechnical Research.2024; 11(3): 161. CrossRef - Microbial‑induced carbonate precipitation (MICP) technology: a review on the fundamentals and engineering applications
Kuan Zhang, Chao-Sheng Tang, Ning-Jun Jiang, Xiao-Hua Pan, Bo Liu, Yi-Jie Wang, Bin Shi
Environmental Earth Sciences.2023;[Epub] CrossRef - Solidification treatment of rare earth tailings by a renewable biological cementation method
Wei-da Wang, Tan Wang, Zhen-bo Sun, Yan-xin Bo, Chang-xiong Zou, Zhe Wang, Chun-li Zheng
Process Safety and Environmental Protection.2023; 179: 585. CrossRef - Biocalcifying Potential of Ureolytic Bacteria Isolated from Soil for Biocementation and Material Crack Repair
Laxmi Leeprasert, Duenrut Chonudomkul, Chanita Boonmak
Microorganisms.2022; 10(5): 963. CrossRef - Microbial Concrete—a Sustainable Solution for Concrete Construction
Parampreet Kaur, Varinder Singh, Amit Arora
Applied Biochemistry and Biotechnology.2022; 194(3): 1401. CrossRef - Effects of Various Pseudomonas Bacteria Concentrations on the Strength and Durability Characteristics of Concrete
Ashish Shukla, Nakul Gupta, Saurav Dixit, Nikolai Ivanovich Vatin, Manish Gupta, Kuldeep Kumar Saxena, Chander Prakash
Buildings.2022; 12(7): 993. CrossRef - Controlling pore-scale processes to tame subsurface biomineralization
Joaquin Jimenez-Martinez, Jen Nguyen, Dani Or
Reviews in Environmental Science and Bio/Technology.2022; 21(1): 27. CrossRef - Advancements in bacteria based self-healing concrete and the promise of modelling
Manpreet Bagga, Charlotte Hamley-Bennett, Aleena Alex, Brubeck L Freeman, Ismael Justo-Reinoso, Iulia C Mihai, Susanne Gebhard, Kevin Paine, Anthony D Jefferson, Enrico Masoero, Irina D Ofiţeru
Construction and Building Materials.2022; 358: 129412. CrossRef - Bio-strengthening of cementitious composites from incinerated sugarcane filter cake by a calcifying bacterium Lysinibacillus sp. WH
Zerlinda Mara Ditta, Nantawat Tanapongpisit, Wittawat Saenrang, Ittipon Fongkaew, Poemwai Chainakun, Wasan Seemakram, Sophon Boonlue, Vanchai Sata, Jindarat Ekprasert
Scientific Reports.2022;[Epub] CrossRef - Performance Evaluation of Bio Concrete by Cluster and Regression Analysis for Environment Protection
Ashish Shukla, Nakul Gupta, Kunwar Raghvendra Singh, Pawan Kumar Verma, Mohit Bajaj, Arfat Ahmad Khan, Frie Ayalew, Anastasios D. Doulamis
Computational Intelligence and Neuroscience.2022; 2022: 1. CrossRef - Agricultural by-products and oyster shell as alternative nutrient sources for microbial sealing of early age cracks in mortar
Minyoung Hong, Indong Jang, Yongjun Son, Chongku Yi, Woojun Park
AMB Express.2021;[Epub] CrossRef - The influence of long-term Zn and Cu contamination in Spolic Technosols on water-soluble organic matter and soil biological activity
Inna V. Zamulina, Andrey V. Gorovtsov, Tatiana M. Minkina, Saglara S. Mandzhieva, Tatiana V. Bauer, Marina V. Burachevskaya
Ecotoxicology and Environmental Safety.2021; 208: 111471. CrossRef - Morpho-Mineralogical and Bio-Geochemical Description of Cave Manganese Stromatolite-Like Patinas (Grotta del Cervo, Central Italy) and Hints on Their Paleohydrological-Driven Genesis
Simone Bernardini, Fabio Bellatreccia, Andrea Columbu, Ilaria Vaccarelli, Marika Pellegrini, Valme Jurado, Maddalena Del Gallo, Cesareo Saiz-Jimenez, Armida Sodo, Christian Millo, Luigi Jovane, Jo De Waele
Frontiers in Earth Science.2021;[Epub] CrossRef - Aerobic non-ureolytic bacteria-based self-healing cementitious composites: A comprehensive review
Ismael Justo-Reinoso, Andrew Heath, Susanne Gebhard, Kevin Paine
Journal of Building Engineering.2021; 42: 102834. CrossRef - Use of Methylcellulose-Based Pellet to Enhance the Bacterial Self-Healing of Cement Composite
Indong Jang, Dasom Son, Yongjun Son, Jihyeon Min, Chongku Yi
Materials.2021; 14(20): 6113. CrossRef - Profiling of Bacteria Capable of Precipitating CaCO3on the Speleothem Surfaces in Dupnisa Cave, Kırklareli, Turkey
Merve Dilek Türkgenci, Nihal Doğruöz Güngör
Geomicrobiology Journal.2021; 38(9): 816. CrossRef - Bacterial Self-Healing Performance of Coated Expanded Clay in Concrete
Sanghyun Han, Indong Jang, Eun Kyung Choi, Woojun Park, Chongku Yi, Namhyun Chung
Journal of Environmental Engineering.2020;[Epub] CrossRef - Biosynthesis and characterization of cadmium carbonate crystals by anaerobic granular sludge capable of precipitate cadmium
C.M. Martínez, M. Rivera-Hernández, Luis H. Álvarez, Ismael Acosta-Rodríguez, F. Ruíz, V.D. Compeán-García
Materials Chemistry and Physics.2020; 246: 122797. CrossRef - Amentoflavone, a novel cyanobacterial killing agent from Selaginella tamariscina
Jaebok Lee, Minkyung Kim, Sang Eun Jeong, Hye Yoon Park, Che Ok Jeon, Woojun Park
Journal of Hazardous Materials.2020; 384: 121312. CrossRef -
Dominance of Gas-Eating, Biofilm-Forming
Methylobacterium
Species in the Evaporator Cores of Automobile Air-Conditioning Systems
Chulwoo Park, Hye Su Jung, Soyoon Park, Che Ok Jeon, Woojun Park, Katherine McMahon
mSphere.2020;[Epub] CrossRef -
Bacillus miscanthi sp. nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus
Bora Shin, Chulwoo Park, Byoung-Hee Lee, Ki-Eun Lee, Woojun Park
International Journal of Systematic and Evolutionary Microbiology
.2020; 70(3): 1843. CrossRef - Effects of spray-dried co-cultured bacteria on cement mortar
Indong Jang, Dasom Son, Wonjae Kim, Woojun Park, Chongku Yi
Construction and Building Materials.2020; 243: 118206. CrossRef - Insights into the Current Trends in the Utilization of Bacteria for Microbially Induced Calcium Carbonate Precipitation
Sing Chuong Chuo, Sarajul Fikri Mohamed, Siti Hamidah Mohd Setapar, Akil Ahmad, Mohammad Jawaid, Waseem A. Wani, Asim Ali Yaqoob, Mohamad Nasir Mohamad Ibrahim
Materials.2020; 13(21): 4993. CrossRef - Optimization of bacterial sporulation using economic nutrient for self-healing concrete
Youngung Ryu, Ki-Eun Lee, In-Tae Cha, Woojun Park
Journal of Microbiology.2020; 58(4): 288. CrossRef - Non-ureolytic microbial self-repairing concrete for low temperature environment
Yilin Su, Jianhang Feng, Qiwei Zhan, Yi Zhang, Chunxiang Qian
Smart Materials and Structures.2019; 28(7): 075041. CrossRef - Biomineralization of carbonates induced by the fungi Paecilomyces inflatus and Plectosphaerella cucumerina
Vincenzo Pasquale, Saverio Fiore, Dhaker Hlayem, Antonio Lettino, F. Javier Huertas, Elena Chianese, Stefano Dumontet
International Biodeterioration & Biodegradation.2019; 140: 57. CrossRef - A Comparison of Bacteria Cultured from Unionid Mussel Hemolymph between Stable Populations in the Upper Mississippi River Basin and Populations Affected by a Mortality Event in the Clinch River
Eric Leis, Sara Erickson, Diane Waller, Jordan Richard, Tony Goldberg
Freshwater Mollusk Biology and Conservation.2019;[Epub] CrossRef - Effectiveness of expanded clay as a bacteria carrier for self-healing concrete
Sanghyun Han, Eun Kyung Choi, Woojun Park, Chongku Yi, Namhyun Chung
Applied Biological Chemistry.2019;[Epub] CrossRef - Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13
Yun Suk Lee, Woojun Park
AMB Express.2019;[Epub] CrossRef - Biomimetic mineralization of calcium carbonate/poly (sodium p-styrenesulfonate) for lysozyme immobilization
Wei Xu, Xiaonan Xue, Lu Huang, Huan Liu, Bakht Ramin Shah, Yuntao Wang, Yingying Li, Xinfang Liu
Materials Research Express.2018; 6(2): 025101. CrossRef - Current challenges and future directions for bacterial self-healing concrete
Yun Suk Lee, Woojun Park
Applied Microbiology and Biotechnology.2018; 102(7): 3059. CrossRef
Research Support, Non-U.S. Gov'ts
- Analysis of the Abilities of Endophytic Bacteria Associated with Banana Tree Roots to Promote Plant Growth
-
Leandro Fernandes Andrade , Gleika Larisse Oliveira Dorasio de Souza , Silvia Nietsche , Adelica Aparecida Xavier , Marcia Regina Costa , Acleide Maria Santos Cardoso , Marlon Cristian Toledo Pereira , Débora Francine Gomes Silva Pereira
-
J. Microbiol. 2014;52(1):27-34. Published online January 4, 2014
-
DOI: https://doi.org/10.1007/s12275-014-3019-2
-
-
48
View
-
0
Download
-
55
Crossref
-
Abstract
-
A total of 40 endophytic bacterial isolates obtained from banana
tree roots were characterized for their biotechnological
potential for promoting banana tree growth. All isolates
had at least one positive feature. Twenty isolates were likely
diazotrophs and formed pellicles in nitrogen-free culture
medium, and 67% of these isolates belonged to the genus
Bacillus sp. The isolates EB-04, EB-169, EB-64, and EB-144
had N fixation abilities as measured by the Kjeldahl method
and by an acetylene reduction activity assay. Among the 40
isolates, 37.5% were capable of solubilizing inorganic phosphate
and the isolates EB-47 and EB-64 showed the highest
solubilization capacity. The isolate EB-53 (Lysinibacillus sp.)
had a high solubilization index, whereas 73% of the isolates
had low solubilization indices. The synthesis of indole-3-
acetic acid (IAA) in the presence of L-tryptophan was detected
in 40% of the isolates. The isolate EB-40 (Bacillus sp.)
produced the highest amount of IAA (47.88 μg/ml) in medium
supplemented with L-tryptophan and was able to synthesize
IAA in the absence of L-tryptophan. The isolates
EB-126 (Bacillus subtilis) and EB-47 (Bacillus sp.) were able
to simultaneously fix nitrogen, solubilize phosphate and produce
IAA in vitro. The results of this study demonstrated
that the isolates analyzed here had diverse abilities and all
have the potential to be used as growth-promoting microbial
inoculants for banana trees.
-
Citations
Citations to this article as recorded by

- Genome sequencing of Bacillus cereus isolate EB-40: a plant growth-promoting bacterium
Débora Francine Gomes Silva Pereira, Pedro Marcus Pereira Vidigal, Samuel A. Santos, Silvia Nietsche, Adelica Aparecida Xavier, Marlon Cristian Toledo Pereira
Discover Applied Sciences.2024;[Epub] CrossRef - Lysinibacillus pinottii sp. nov., a novel species with anti-mosquito and anti-mollusk activity
Christopher A. Dunlap, Eric T. Johnson, Marleny Burkett-Cadena, Johanna Cadena, Ephantus J. Muturi
Antonie van Leeuwenhoek.2024;[Epub] CrossRef - Plant endophytes: unveiling hidden applications toward agro-environment sustainability
Rajeshwari Negi, Babita Sharma, Sanjeev Kumar, Kundan Kumar Chaubey, Tanvir Kaur, Rubee Devi, Ashok Yadav, Divjot Kour, Ajar Nath Yadav
Folia Microbiologica.2024; 69(1): 181. CrossRef - Lysinibacillus piscis sp. nov. isolated from the gut of mottled spinefoot Siganus fuscescens
Hiyu Kanbe, Yuki Sano, Kazumori Mise, Shusei Kanie, Natsumi Ushijima, Keisuke Kawano, Minoru Kihara, Hideomi Itoh
Archives of Microbiology.2024;[Epub] CrossRef - Control ofMeloidogyne javanicain banana by endophytic bacteria
Gleika Larisse Oliveira Dorásio de Souza, Regina Cássia Ferreira Ribeiro, Adelica Aparecida Xavier, Silvia Nietsche, Thaís de Castro Moreira, Maria Josiane Martins, Samy Pimenta, Edson Hiydu Mizobutsi, José Augusto dos Santos Neto, Isabela Oliveira Santos
Open Agriculture.2024;[Epub] CrossRef - Interaction of Mineral Nutrients and Plant Growth-Promoting Microbes for Biofortification of Different Cropping Systems
Arshad Jalal, Carlos Eduardo da Silva Oliveira, Isabela Martins Bueno Gato, Vitória de Almeida Moreira, Bruno Horschut de Lima, Andréa de Castro Bastos, Babar Iqbal, Marcelo Carvalho Minhoto Teixeira Filho
Journal of Plant Growth Regulation.2024;[Epub] CrossRef - Co-Inoculation of Azospirillum brasilense and Bacillus sp. Enhances Biomass and Photosynthetic Efficiency in Urochloa brizantha
Lorena Emanuelle da Mata Terra, Marinalva Martins dos Santos, Maria Carolina Soares Lopes, Daiane Antunes Pinheiro, Érika Manuela Gonçalves Lopes, Amanda Santos Soares, Thiago Gomes dos Santos Braz, Silvia Nietsche, Junio Cota
Agriculture.2024; 14(12): 2349. CrossRef - Molecular and biochemical responses of sesame (Sesame indicum L.) to rhizobacteria inoculation under water deficit
Anderson Reges dos Santos, Geisenilma Maria Gonçalves da Rocha, Alexandre Paulo Machado, Paulo Ivan Fernandes-Junior, Nair Helena Castro Arriel, Tarcisio Marcos de Souza Gondim, Liziane Maria de Lima
Frontiers in Plant Science.2024;[Epub] CrossRef - Tomato plant residues and Bacillus subtilis application can improve the soil environment and increase tomato yield and quality
Aokun Shi, Qinghua Di, Kunhao Xie, Xiaojie Feng, Xiaoqin Wang, Yansu Li, Mintao Sun, Xianchang Yu, Jun Wang, Li Han, Chaoxing He
Technology in Horticulture.2024;[Epub] CrossRef - Comparison of Rhizospheric and Endophytic Bacterial Compositions between Netted and Oriental Melons
Jian Xiao, Yan Sun, Yi He, Xiaofu Tang, Shangdong Yang, Jinyan Huang, Jing Han
Microbiology Spectrum.2023;[Epub] CrossRef - Salt tolerance of endophytic root bacteria and their effects on seed germination and viability on tomato plants
Mustafa Akbaba, Eren Özden
Brazilian Journal of Microbiology.2023; 54(4): 3147. CrossRef - Lysinibacillus spp.: an IAA-producing endospore forming-bacteria that promotes plant growth
Manuel Pantoja-Guerra, Marleny Burkett-Cadena, Johanna Cadena, Christopher A. Dunlap, Camilo A. Ramírez
Antonie van Leeuwenhoek.2023; 116(7): 615. CrossRef - Optimal growth and N use efficiency enhancements by growth-promoting rhizobacteria in seedlings banana under N2 deficiency
Flávia Melo Moreira, Paulo Araquém Ramos Cairo, Lucas Ribeiro do Nascimento, Raul Castro Carriello Costa Rosa, Leandro de Souza Rocha, Fernando Haddad
Biocatalysis and Agricultural Biotechnology.2023; 50: 102734. CrossRef - Response of endophytic bacteria in sugarcane roots to different slow-release fertilizers with dicyandiamide (DCD) and humic acid (HA) applications
Jian Xiao, Siyu Chen, Tian Liang, Shangdong Yang, Hongwei Tan
Environmental Technology & Innovation.2023; 32: 103244. CrossRef - Plant growth-promoting activities of bacterial endophytes isolated from the medicinal plant Pairs polyphylla var. yunnanensis
Liu Tao, Liao Qiuhong, Yu Fuqiang, Zi Shuhui, Tian Suohui, Fan Linyuan
World Journal of Microbiology and Biotechnology.2022;[Epub] CrossRef - Authentication of putative competitive bacterial endophytes of rice by re-isolation and DNA fingerprinting assay
Sai Aparna Devi Nunna, Dananjeyan Balachandar
Journal of Applied Microbiology.2022; 133(3): 1808. CrossRef - Autochthonous endophytic bacteria from Musa sp. controls Fusarium oxysporum f. sp. cubense under in vitro conditions
MARIA J. MARTINS, ADELICA A. XAVIER, ISABELLE C. CARDOSO, DANIELA F. SILVEIRA, REGINA C.F. RIBEIRO, SAMY PIMENTA, SILVIA NIETSCHE
Anais da Academia Brasileira de Ciências.2022;[Epub] CrossRef - Differences of rhizospheric and endophytic bacteria are recruited by different watermelon phenotypes relating to rind colors formation
Jian Xiao, Si-yu Chen, Yan Sun, Shang-dong Yang, Yi He
Scientific Reports.2022;[Epub] CrossRef - Growth and production of ‘Prata Anã Gorutuba’ banana under different planting densities
Célia Lúcia Siqueira, Marlon Cristian Toledo Pereira, Silvia Nietsche, Adelica Aparecida Xavier, Athos Henrique Mendes, Helisson Robert Araújo Xavier, Railton de Souza Guimarães, Danilo Reis Lacerda, Silvânio Rodrigues dos Santos
Scientia Agricola.2021;[Epub] CrossRef - Harnessing Bacterial Endophytes for Promotion of Plant Growth and Biotechnological Applications: An Overview
Ahmed M. Eid, Amr Fouda, Mohamed Ali Abdel-Rahman, Salem S. Salem, Albaraa Elsaied, Ralf Oelmüller, Mohamed Hijri, Arnab Bhowmik, Amr Elkelish, Saad El-Din Hassan
Plants.2021; 10(5): 935. CrossRef - A comprehensive synthesis unveils the mysteries of phosphate‐solubilizing microbes
Jin‐tian Li, Jing‐li Lu, Hong‐yu Wang, Zhou Fang, Xiao‐juan Wang, Shi‐wei Feng, Zhang Wang, Ting Yuan, Sheng‐chang Zhang, Shu‐ning Ou, Xiao‐dan Yang, Zhuo‐hui Wu, Xiang‐deng Du, Ling‐yun Tang, Bin Liao, Wen‐sheng Shu, Pu Jia, Jie‐Liang Liang
Biological Reviews.2021; 96(6): 2771. CrossRef - Maize-associated bacteria from the Brazilian semiarid region boost plant growth and grain yield
Rejane de Carvalho Nascimento, Maria Idaline Pessoa Cavalcanti, Andreza de Jesus Correia, Indra Elena Costa Escobar, Ana Dolores Santiago de Freitas, Rafaela Simão Abrahão Nóbrega, Paulo Ivan Fernandes-Júnior
Symbiosis.2021; 83(3): 347. CrossRef - Genetic diversity and promotion plant growth of culturable endophytic diazotrophs associated with seashore paspalum cultivars
Tianzeng Liu, Chaonan Zhai, Juming Zhang, Jeffrey A. Coulter
New Zealand Journal of Crop and Horticultural Science.2021; 49(2-3): 243. CrossRef - Probiotic Endophytes for More Sustainable Banana Production
Miguel J. Beltran-Garcia, America Martinez-Rodriguez, Ileana Olmos-Arriaga, Benjamin Valdez-Salas, Yur Y. Chavez-Castrillon, Paolo Di Mascio, James F. White
Microorganisms.2021; 9(9): 1805. CrossRef - Structural and chemical characterization of hop bine fibers and their applications in the paper industry
Kurt J. Haunreiter, Anthony Dichiara, Rick Gustafson
Industrial Crops and Products.2021; 174: 114217. CrossRef - Lysinibacillus Species: Their Potential as Effective Bioremediation, Biostimulant, and Biocontrol Agents
Nusrat Ahsan, Masafumi Shimizu
Reviews in Agricultural Science.2021; 9: 103. CrossRef - Characterization of a Versatile Plant Growth-Promoting Rhizobacterium Pseudomonas mediterranea Strain S58
Yilin Gu, Jing Wang, Zhenyuan Xia, Hai-Lei Wei
Microorganisms.2020; 8(3): 334. CrossRef - Isolation, Selection and Characterization of Root-Associated Rock Phosphate Solubilizing Bacteria in Moroccan Wheat (Triticum aestivum L.)
Abderrazak Rfaki, Omar Zennouhi, Fatima Zahra Aliyat, Laila Nassiri, Jamal Ibijbijen
Geomicrobiology Journal.2020; 37(3): 230. CrossRef - Bacillus cereus bacteria endófita promotora de crecimiento vegetal
Lina Maria Chamorro Anaya, Leonado Miguel Chamorro Anaya, Alexander Perez Cordero
Revista Colombiana de Biotecnología.2020; 22(2): 18. CrossRef - Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir)
Sonia Maria Lima Santos do Vale, Amauri Siviero, Lauro Saraiva Lessa, Eduardo Pacca Luna Mattar, Paulo Arthur Almeida do Vale
AIMS Agriculture and Food.2020; 5(4): 850. CrossRef - Isolation of endophytic bacteria from Rehmannia glutinosa Libosch and their potential to promote plant growth
Shanshan Wang, Baoyu Ji, Xiuhong Su, Hanwei Li, Chengming Dong, Suiqing Chen, Yunhao Zhu, Weisheng Feng
The Journal of General and Applied Microbiology.2020; 66(5): 279. CrossRef - Çeşitli Kültür Bitkilerinden İzole Edilen Endofitik Bakterilerin Karakterizasyonu ve Bitki Patojeni Bakterilere Karşı Antagonistik Etkilerinin Belirlenmesi
Yasin BABİER, Ahmet AKKÖPRÜ
Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi.2020; 30(3): 521. CrossRef - Engineering banana endosphere microbiome to improve Fusarium wilt resistance in banana
Yupei Liu, Aiping Zhu, Hongming Tan, Lixiang Cao, Renduo Zhang
Microbiome.2019;[Epub] CrossRef - Endophytic interaction of Bacillus sp. in micropropagated banana plantlets
JOSIELE S. ROCHA, SILVIA NIETSCHE, MARLON C.T. PEREIRA, MERCADANTE-SIMÕES MARIA O., RAYANE C. SANTOS, ADELICA A. XAVIER
Anais da Academia Brasileira de Ciências.2019;[Epub] CrossRef - Lysinibacillus capsici sp. nov, isolated from the rhizosphere of a pepper plant
Marleny Burkett-Cadena, Leonardo Sastoque, Johanna Cadena, Christopher A. Dunlap
Antonie van Leeuwenhoek.2019; 112(8): 1161. CrossRef - Culturable endophytic bacteria ofCamelliaspecies endowed with plant growth promoting characteristics
A. Borah, R. Das, R. Mazumdar, D. Thakur
Journal of Applied Microbiology.2019; 127(3): 825. CrossRef - Endophytes isolated from ginger rhizome exhibit growth promoting potential forZea mays
Yu Zhang, Xia Kang, Hanjun Liu, Yihao Liu, Yanmei Li, Xiumei Yu, Ke Zhao, Yunfu Gu, Kaiwei Xu, Cuiping Chen, Qiang Chen
Archives of Agronomy and Soil Science.2018; 64(9): 1302. CrossRef - Bioprospecting and selection of growth-promoting bacteria for Cymbidium sp. orchids
Júlia Brandão Gontijo, Gracielle Vidal Silva Andrade, Marihus Altoé Baldotto, Lílian Estrela Borges Baldotto
Scientia Agricola.2018; 75(5): 368. CrossRef - Evaluation of the plant-growth-promoting abilities of endophytic bacteria from the psammophyteAmmodendron bifolium
Yanlei Zhu, Xiaoping She
Canadian Journal of Microbiology.2018; 64(4): 253. CrossRef - Mangrove endophyte promotes reforestation tree ( Acacia polyphylla ) growth
Renata Assis Castro, Manuella Nóbrega Dourado, Jaqueline Raquel de Almeida, Paulo Teixeira Lacava, André Nave, Itamar Soares de Melo, João Lucio de Azevedo, Maria Carolina Quecine
Brazilian Journal of Microbiology.2018; 49(1): 59. CrossRef - Screening of plant growth promotion ability among bacteria isolated from field-grown sorghum under different managements in Brazilian drylands
Jéssica Fernanda da Silva, Thaise Rosa da Silva, Indra Elena Costa Escobar, Ana Carla Resende Fraiz, Jonnathan Whiny Moraes dos Santos, Tailane Ribeiro do Nascimento, João Marcos Rodrigues dos Santos, Samuel James Windsor Peters, Roseli Freire de Melo, Di
World Journal of Microbiology and Biotechnology.2018;[Epub] CrossRef - Phosphate solubilization by endophytic bacteria isolated from banana trees
AMANDA D.M. MATOS, IZABELA C.P. GOMES, SILVIA NIETSCHE, ADELICA A. XAVIER, WELLINGTON S. GOMES, JOSÉ A. DOS SANTOS NETO, MARLON C.T. PEREIRA
Anais da Academia Brasileira de Ciências.2017; 89(4): 2945. CrossRef - ENDOPHYTIC BACTERIA USED AS BIOINOCULANTS IN MICROPROPAGATED BANANA SEEDLINGS
GLEIKA LARISSE OLIVEIRA DORASIO DE SOUZA, DEIVISSON FERREIRA DA SILVA, SILVIA NIETSCHE, ADELICA APARECIDA XAVIER, MARLON CRISTIAN TOLEDO PEREIRA
Revista Brasileira de Fruticultura.2017;[Epub] CrossRef - Temporal dynamics in microbial soil communities at anthrax carcass sites
Karoline Valseth, Camilla L. Nesbø, W. Ryan Easterday, Wendy C. Turner, Jaran S. Olsen, Nils Chr. Stenseth, Thomas H. A. Haverkamp
BMC Microbiology.2017;[Epub] CrossRef - Biocontrol of the internalization of Salmonella enterica and Enterohaemorrhagic Escherichia coli in mung bean sprouts with an endophytic Bacillus subtilis
Zhenyu Shen, Azlin Mustapha, Mengshi Lin, Guolu Zheng
International Journal of Food Microbiology.2017; 250: 37. CrossRef - Shoot the Message, Not the Messenger—Combating Pathogenic Virulence in Plants by Inhibiting Quorum Sensing Mediated Signaling Molecules
Ganesh Alagarasan, Kumar S. Aswathy, Munusamy Madhaiyan
Frontiers in Plant Science.2017;[Epub] CrossRef - Isolation of phosphate solubilizing endophytic bacteria from Phyllanthus amarus Schum & Thonn: Evaluation of plant growth promotion and antioxidant activity under salt stress
Manoharan Melvin Joe, Shalini Devaraj, Abitha Benson, Tongmin Sa
Journal of Applied Research on Medicinal and Aromatic Plants.2016; 3(2): 71. CrossRef - Diversity of indigenous endophytic bacteria associated with the roots of Chinese cabbage (Brassica campestris L.) cultivars and their antagonism towards pathogens
Md. Azizul Haque, Han Dae Yun, Kye Man Cho
Journal of Microbiology.2016; 54(5): 353. CrossRef - Triple combinations with PGPB stimulate plant growth in micropropagated banana plantlets
Gleika L.O.D. Souza, Silvia Nietsche, Adelica A. Xavier, Márcia R. Costa, Marlon C.T. Pereira, Márcio A. Santos
Applied Soil Ecology.2016; 103: 31. CrossRef - Bacillus amyloliquefaciens Strain W19 can Promote Growth and Yield and Suppress Fusarium Wilt in Banana Under Greenhouse and Field Conditions
Beibei WANG, Zongzhuan SHEN, Fengge ZHANG, Waseem RAZA, Jim YUAN, Rong HUANG, Yunze RUAN, Rong LI, Qirong SHEN
Pedosphere.2016; 26(5): 733. CrossRef - Novel components of leaf bacterial communities of field-grown tomato plants and their potential for plant growth promotion and biocontrol of tomato diseases
Fernando M. Romero, María Marina, Fernando L. Pieckenstain
Research in Microbiology.2016; 167(3): 222. CrossRef - Oasis desert farming selects environment‐specific date palm root endophytic communities and cultivable bacteria that promote resistance to drought
Hanene Cherif, Ramona Marasco, Eleonora Rolli, Raoudha Ferjani, Marco Fusi, Asma Soussi, Francesca Mapelli, Ikram Blilou, Sara Borin, Abdellatif Boudabous, Ameur Cherif, Daniele Daffonchio, Hadda Ouzari
Environmental Microbiology Reports.2015; 7(4): 668. CrossRef - Indole: a signaling molecule or a mere metabolic byproduct that alters bacterial physiology at a high concentration?
Jisun Kim, Woojun Park
Journal of Microbiology.2015; 53(7): 421. CrossRef - Phytostimulatory and hardening period-reducing effects of plant-associated bacteria on micropropagated Musa acuminata cv. Grand Naine
E. P. Suada, B. Jasim, C. J. Jimtha, G. P. Gayatri, E. K. Radhakrishnan, A. Remakanthan
In Vitro Cellular & Developmental Biology - Plant.2015; 51(6): 682. CrossRef - Diversity, bioactivities, and metabolic potentials of endophytic actinomycetes isolated from traditional medicinal plants in Sichuan, China
Peng QIU, Zhi-Xiang FENG, Jie-Wei TIAN, Zu-Chao LEI, Lei WANG, Zhi-Gang ZENG, Yi-Wen CHU, Yong-Qiang TIAN
Chinese Journal of Natural Medicines.2015; 13(12): 942. CrossRef
- Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Wheat Roots by Wheat Germ Agglutinin Labeled with Fluorescein Isothiocyanate
-
Jian Zhang , Jingyang Liu , Liyuan Meng , Zhongyou Ma , Xinyun Tang , Yuanyuan Cao , Leni Sun
-
J. Microbiol. 2012;50(2):191-198. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1472-3
-
-
41
View
-
0
Download
-
34
Crossref
-
Abstract
-
Thirty-two isolates were obtained from wheat rhizosphere
by wheat germ agglutinin (WGA) labeled with fluorescein
isothiocyanate (FITC). Most isolates were able to produce
indole acetic acid (65.6%) and siderophores (59.3%), as well
as exhibited phosphate solubilization (96.8%). Fourteen isolates
displayed three plant growth-promoting traits. Among
these strains, two phosphate-dissolving ones, WS29 and
WS31, were evaluated for their beneficial effects on the early
growth of wheat (Triticum aestivum Wan33). Strain WS29
and WS31 significantly promoted the development of lateral
roots by 34.9% and 27.6%, as well as increased the root dry
weight by 25.0% and 25.6%, respectively, compared to those
of the control. Based on 16S rRNA gene sequence comparisons
and phylogenetic positions, both isolates were determined
to belong to the genus Bacillus. The proportion of
isolates showing the properties of plant growth-promoting
rhizobacteria (PGPR) was higher than in previous reports.
The efficiency of the isolation of PGPR strains was also
greatly increased by WGA labeled with FITC. The present
study indicated that WGA could be used as an effective tool
for isolating PGPR strains with high affinity to host plants
from wheat roots. The proposed approach could facilitate
research on biofertilizers or biocontrol agents.
-
Citations
Citations to this article as recorded by

- Parameter evaluation for developing phosphate-solubilizing Bacillus inoculants
Daniel Bini, Bianca Braz Mattos, José Edson Fontes Figueiredo, Flávia Cristina dos Santos, Ivanildo Evodio Marriel, Cristiane Alcantara dos Santos, Christiane Abreu de Oliveira-Paiva
Brazilian Journal of Microbiology.2024; 55(1): 737. CrossRef - Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of major crops grown in Marathwada region of Maharashtra, India
Sharda S. Gavande, Anuj Maurya, Shashikant Sharma
Vegetos.2024; 37(2): 637. CrossRef - Multifaceted growth promotion and biocontrol of Agroathelia rolfsii and induction of defense mechanism by Bacillus amyloliquefaciensSS‐CR10 on chilli
Chandagalu Ramesh Santhosh, Shivannegowda Mahadevakumar, Bettadapura Rameshgowda Nuthan, Madeva Bharatha, Javaraiah Parashiva, Madappa Mahesh, Siddaiah Chandranayaka, Sreedharamurthy Satish
Physiologia Plantarum.2024;[Epub] CrossRef - Isolation of Endophytic Fungi from Halophytic Plants and their Identification and Screening for Auxin Production and Other Plant Growth Promoting Traits
Muhammad Ikram, Niaz Ali, Gul Jan, Farzana Gul Jan, Rainaz Pervez, Muhammad Romman, Rimsha Zainab, Humaira Yasmin, Naeem Khan
Journal of Plant Growth Regulation.2023; 42(8): 4707. CrossRef - The Effects of Seed Pretreatment with Endophytic Bacteria Bacillus subtilis on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit
Alsu R. Lubyanova, Chulpan R. Allagulova, Oksana V. Lastochkina
Plants.2023; 12(14): 2684. CrossRef - Phosphate-Solubilizing Pseudomonas sp. Strain WS32 Rhizosphere Colonization-Induced Expression Changes in Wheat Roots
Kangmiao Ou, Xiangyi He, Ke Cai, Weirong Zhao, Xiaoxun Jiang, Wenfeng Ai, Yue Ding, Yuanyuan Cao
Frontiers in Microbiology.2022;[Epub] CrossRef - Enhanced anti-herbivore defense of tomato plants against Spodoptera litura by their rhizosphere bacteria
Sumei Ling, Yi Zhao, Shaozhi Sun, Dong Zheng, Xiaomin Sun, Rensen Zeng, Dongmei Chen, Yuanyuan Song
BMC Plant Biology.2022;[Epub] CrossRef - Recent Progress in Identifying Bacteria with Fluorescent Probes
Yuefeng Ji, Guanhao Li, Juan Wang, Chunxiang Piao, Xin Zhou
Molecules.2022; 27(19): 6440. CrossRef - Cenchrus setigerus Vahl. secretes root agglutinins to promote colonization by plant growth-promoting rhizobacteria
Subhashree Subramanyam, Nagesh Sardesai, Cherukeri R. Babu
Brazilian Journal of Botany.2022; 45(2): 819. CrossRef - The impact of newly synthesized sulfonamides on soil microbial population and respiration in rhizospheric soil of wheat (Triticum aestivum L.)
Ammara Saleem, Asma Zulfiqar, Muhammad Zeeshan Arshed, Saber Hussain, Muhammad Tajammal Khan, Marek Zivcak, Ali Tan Kee Zuan, Shrouq M. Alshahrani, Khaloud Mohammed Alarjani, Rahul Datta
PLOS ONE.2022; 17(4): e0264476. CrossRef - Structural and functional characterization of rhizosphere actinomycetes of major crop plants under shifting cultivation practice in Northeast India
Marcy D. Momin, Ngangbam Somen Singh, Awadhesh Kumar, Shri Kant Tripathi
Vegetos.2021; 34(3): 638. CrossRef - Development of fluorescent probes targeting the cell wall of pathogenic bacteria
Weiwei Liu, Lu Miao, Xiaolian Li, Zhaochao Xu
Coordination Chemistry Reviews.2021; 429: 213646. CrossRef - The Isolation and Characterization of Phosphate Solubilizing Bacteria from the Onion Rhizosphere and Their Effect on Onion Growth
İdris BEKTAŞ, Mustafa KÜSEK
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi.2021; 24(5): 1084. CrossRef - Defining the wheat microbiome: Towards microbiome-facilitated crop production
Vanessa N. Kavamura, Rodrigo Mendes, Adnane Bargaz, Tim H. Mauchline
Computational and Structural Biotechnology Journal.2021; 19: 1200. CrossRef - Arsenic Remediation in Bangladeshi Rice Varieties with Enhance Plant Growth by Unique Arsenic-Resistant Bacterial Isolates
Md. Shahedur Rahman, Mohammad Abu Hena Mostofa Jamal, Polash Kumar Biswas, Shaikh Mizanur Rahman, Satya Priya Sharma, Subbroto Kumar Saha, Seong Tshool Hong, Md. Rezuanul Islam
Geomicrobiology Journal.2020; 37(2): 130. CrossRef - Shifts Between and Among Populations of Wheat Rhizosphere Pseudomonas, Streptomyces and Phyllobacterium Suggest Consistent Phosphate Mobilization at Different Wheat Growth Stages Under Abiotic Stress
Claudia Breitkreuz, François Buscot, Mika Tarkka, Thomas Reitz
Frontiers in Microbiology.2020;[Epub] CrossRef - Amelioration of drought effects in wheat and cucumber by the combined application of super absorbent polymer and potential biofertilizer
Yongbin Li, Haowen Shi, Haowei Zhang, Sanfeng Chen
PeerJ.2019; 7: e6073. CrossRef - Isolation, characterization and molecular identification of a halotolerant Bacillus megaterium CTBmeg1 able to grow on halogenated compounds
Kazim Akcay, Yilmaz Kaya
Biotechnology & Biotechnological Equipment.2019; 33(1): 945. CrossRef - Biodegradation of di-n-butyl phthalate (DBP) by a novel endophytic Bacillus megaterium strain YJB3
Nai-Xian Feng, Jiao Yu, Ce-Hui Mo, Hai-Ming Zhao, Yan-Wen Li, Bing-Xiao Wu, Quan-Ying Cai, Hui Li, Dong-Mei Zhou, Ming-Hung Wong
Science of The Total Environment.2018; 616-617: 117. CrossRef - Characterization of plant growth promoting rhizobacteria (PGPR) isolated from the rhizosphere of Vigna radiata (mung bean)
Punam Kumari, Mukesh Meena, R.S. Upadhyay
Biocatalysis and Agricultural Biotechnology.2018; 16: 155. CrossRef - Characterization of diazotrophic growth-promoting rhizobacteria isolated from ginger root soil as antagonists against Ralstonia solanacearum
Jian Zhang, Tingting Guo, Pengcheng Wang, Hongmei Tian, Yan Wang, Jingyi Cheng
Biotechnology & Biotechnological Equipment.2018; 32(6): 1447. CrossRef - Green mitigation strategy for cultural heritage: bacterial potential for biocide production
Mara Silva, Tânia Rosado, Dora Teixeira, António Candeias, Ana Teresa Caldeira
Environmental Science and Pollution Research.2017; 24(5): 4871. CrossRef - Screening of Rhizospheric Actinomycetes for Various In-vitro and In-vivo Plant Growth Promoting (PGP) Traits and for Agroactive Compounds
Sumaira Anwar, Basharat Ali, Imran Sajid
Frontiers in Microbiology.2016;[Epub] CrossRef - A Complex Inoculant of N2-Fixing, P- and K-Solubilizing Bacteria from a Purple Soil Improves the Growth of Kiwifruit (Actinidia chinensis) Plantlets
Hong Shen, Xinhua He, Yiqing Liu, Yi Chen, Jianming Tang, Tao Guo
Frontiers in Microbiology.2016;[Epub] CrossRef - Assessment of the natural endophytic association between Rhizobium and wheat and its ability to increase wheat production in the Nile delta
Youssef G. Yanni, Frank B. Dazzo, Andrea Squartini, Marina Zanardo, Mohamed I. Zidan, Abd Elgawad Y. Elsadany
Plant and Soil.2016; 407(1-2): 367. CrossRef - Involvement of soil bacteria in ABO blood mistyping
Naoki Takada, Chikahiro Mori, Rie Takai, Tomohiro Takayama, Yoshihisa Watanabe, Kohei Nakamura, Kazuhiro Takamizawa
Legal Medicine.2015; 17(2): 128. CrossRef - Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion
Afshan Majeed, M. Kaleem Abbasi, Sohail Hameed, Asma Imran, Nasir Rahim
Frontiers in Microbiology.2015;[Epub] CrossRef - Characterization of bacterial strains and their impact on plant growth promotion and yield of wheat and microbial populations of soil
Kumar Ashok, Ram Maurya Bihari, Raghuwanshi Richa
African Journal of Agricultural Research.2015; 10(12): 1367. CrossRef - Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.)
Mahwish Zahid, M. Kaleem Abbasi, Sohail Hameed, Nasir Rahim
Frontiers in Microbiology.2015;[Epub] CrossRef - The role of wheat germ agglutinin in the attachment of Pseudomonas sp. WS32 to wheat root
Jian Zhang, Liyuan Meng, Yuanyuan Cao, Huiping Chang, Zhongyou Ma, Leni Sun, Ming Zhang, Xinyun Tang
Journal of Microbiology.2014; 52(12): 1020. CrossRef - Screening of plant growth-promoting traits in arsenic-resistant bacteria isolated from agricultural soil and their potential implication for arsenic bioremediation
Suvendu Das, Jiin-Shuh Jean, Sandeep Kar, Mon-Lin Chou, Chien-Yen Chen
Journal of Hazardous Materials.2014; 272: 112. CrossRef - Screening of Antagonistic Bacteria having Antifungal Activity against Various Phytopathogens
Hee-Jong Yang, Su-Ji Jeong, Seong-Yeop Jeong, Do-Youn Jeong
The Korean Journal of Mycology.2014; 42(4): 333. CrossRef - The areas of application for plant lectins
N. M. Melnykova, L. M. Mykhalkiv, P. M. Mamenko, S. Ya. Kots
Biopolymers and Cell.2013; 29(5): 357. CrossRef - Diversity and Antimicrobial Activity of Actinomycetes Isolated from Rhizosphere of Rice (Oryza sativa L.)
Hye-Won Lee, Jae-Hyung Ahn, Hang-Yeon Weon, Jaekyeong Song, Byung-Yong Kim
The Korean Journal of Pesticide Science.2013; 17(4): 371. CrossRef
- Effect of copper on the growth and methanol dehydrogenase activity of methylobacillusd sp. strain SK1 DSM 8269
-
Kim, Si W. , Kim, Young M.
-
J. Microbiol. 1996;34(2):172-178.
-
-
-
Abstract
-
Methylobacillus sp. strain SK1, which grows only on methanol, was found to grow in the absence of added copper. The doubling time (t_d = 1.3 h) of the bacterium growing at the exponential growth phase at 30℃ in the absence of copper was the same as that of the cell growing in the presence of copper. The bacterium growing after the exponential phase in the absence of copper, however, grew faster than the cell growing in the presence of copper. Cells harvested after thee early stationary phase in the presence of copper were found to exhibit no methanol dehydrogenase (MDH) activity, but the amount and subunit structure of the enzyme in the cells were almost the same as that in cells harboring active MDH. Pellets of the cells harvested after the early stationary phase in the presence of copper were pale green. Cell-free extracts prepared from cells harvested at the early stationary phase in the presence of copper were pink and exhibited MDH activity, but it turned dark-green rapidly from the surface under air. The green-colored portions of the extracts showed no MDH activity and contained c-type cytochromes that were oxidized completely. The inactive MDH activity and contained c-type cytochromes that were oxidized completely. The inactive MDH proteins in the green portions were found to have antigenic sites identical to those of the active one as the inactive MDHs in cells grown in the presence of copper. The bacterium was found to accumulate copper actively during the exponential growth phase. MDH prepared from cells grown in the presence or absence of copper was found to be more stable under nitrogen gas than under air. Methanol at 10 mM was found to enhance the stability of the MDH under air.
- Isolation and characterization of Bacillus sp. KD1014 producing carboxymethyl-cellulase
-
Lee, Kyung Dong , Kim, Jung Ho , Kim Hoon
-
J. Microbiol. 1996;34(4):305-310.
-
-
-
Abstract
-
A microorganism producing carboxymethyl-cellulase (CMCase) was isoalted from 300 soil and compost samples. The isolated was identified as Bacillus sp. by Biolog^TM test and fatty acid analysis, and named as Bacillus sp. KD1014. The isolate could degrade, in addition to CMC, various kinds of polysaccharides such as levan, xylan, starch, and filter paper but hardly degrade microcrystaline Avicel. The optimum growth and CMCase production of the isolate was observed between 16-and 25 hr-culture at 45℃ and pH 5.0. The maximum CMCase activity was observed at pH 4.5 and 60℃. The CMCase was found to bind to Avicel. The CMCase was internally cleaved as growth continued. When crude supernatant was used for activity staining, three major bands were detected on a native gel, however, only on major band was detected on a denaturating gel after removal of the detergent.
- A possible mechanism responsible for translocation and secretion an alkaliphilic bacillus sp. S-1 pullulanase
-
Shim, Jae Kyoung , Kim, Kyoung Sook , Kim, Cheorl Ho
-
J. Microbiol. 1997;35(3):213-221.
-
-
-
Abstract
-
The secretion of the alkaliphilic Bacillus sp. S-1 extracellular pullulanase involves translocation across the cytoplasmic membrane of the Gram-positive bacterial cell envelope. Translocation of the intracellular pullulanase PUL-I, was traced to elucidate the mechanism and pathway of protein secretion from an alkaliphilic Bacillus sp. S-1. Pullulanase could be slowly but quantitatively released into the medium during growth of the cells in medium containing proteinase K. The released pullulanase lacked the N-terminal domain. The N-terminus is the sole membrane anchor in the pullulanase protein and was not affected by proteases, confirming that it is not exposed on the cell surface. Processing of a 180,000M_r pullulanase to a 140,000M_r polypeptide has been demonstrated in cell extracts using antibodies raised against 140,000M_r extracellular form. Processing of the 180,000 M_r protein occured during the preparation of extracts in an alkaline pH condition. A modified rapid extraction procedure suggested that the processing event also occured in vivo. Processing apparently increased the activity of pullulanase. The western blotting analysis with mouse anti-serum against 140-kDa extracellular pullulanase PUL-E showed that PUL-I is processed into PUL-X via intermediate form of PUL-E. Possible explanationa for the translocation are discussed.