Research Support, Non-U.S. Gov't
- Antiviral effects of Lactobacillus ruminis SPM0211 and Bifidobacterium longum SPM1205 and SPM1206 on rotavirus-infected Caco-2 cells and a neonatal mouse model
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Joo Yeon Kang , Do Kyung Lee , Nam Joo Ha , Hea Soon Shin
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J. Microbiol. 2015;53(11):796-803. Published online October 28, 2015
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DOI: https://doi.org/10.1007/s12275-015-5302-2
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Abstract
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Rotavirus is worldwide cause of severe gastroenteritis including
severe diarrhea and fatal dehydration in infants
and young children. There is an available vaccination program
for preventing rotavirus infection, but it has limits
and restrictions. Probiotics therapy could be an alternative
method
of antiviral prevention and modulation against rotavirus
infection. In this study, we screened the antiviral activity
of probiotic bacteria such as 3 Lactobacillus spp. and
14 Bifidobacterium spp. isolated from young Korean. Three
of the bacteria, Lactobacillus ruminis SPM0211, Bifidobacterium
longum SPM1205, and SPM1206, inhibited human
strain Wa rotavirus infection in Caco-2 cells. Furthermore,
these bacterial strains inhibited rotavirus replication in a
rotavirus-infected neonatal mouse model. To clarify the mechanism
of inhibition, we investigated gene expression of
Interferon (IFN)-signaling components and IFN-inducible
antiviral effectors. All 3 probiotics increased IFN-α and IFN-
β levels compared with the control. Gene expression of IFNsignaling
components and IFN-inducible antiviral effectors
also increased. Overall, these results indicate that L. ruminis
SPM0211, B. longum SPM1205 and 1206 efficiently inhibit
rotavirus replication in vitro and in vivo. Especially, the antiviral
effect of Lactobacillus ruminis SPM0211 is worthy of
notice. This is the first report of L. ruminis with antiviral activity.
Anti-rotaviral effects of the 3 probiotics are likely due
to their modulation of the immune response through promoting
type I IFNs, which are key regulators in IFN signaling
pathway.
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- Inhibitory Effects of Lactic Acid Bacteria (LAB) on the Azoxymethane-induced Colonic Preneoplastic Lesions
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Sang-Myeong Lee , Wan-Kyu Lee
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J. Microbiol. 2000;38(3):169-175.
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Abstract
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Epidemiological and experimental studies provide evidences that diet and intestinal microflora play an important role in colon carcinogenesis. In recent years, it has been suggested that lactic acid bacteria (LAB) used to ferment dairy products have an inhibitory effect on the colon cancer. This study was designed to determine the effect of Bifidobacterium longum HY8001 (Bif) and Lactobacillus acidophilus HY2104 (Lac) of Korean origin on azoxymethane (AOM)-induced colonic preneoplastic lesions such as aberrant crypt foci (ACF) formation and cecal pH. At five weeks of age, Spraque-Dawley rats were divided at random into four (AOM alone, Bif, Lac, and Bif+Lac) groups. Animals were weighed weekly and oral administration of LAB cultures were performed daily until the termination of the study. Two weeks later, all animals were given a subcutaneous injection of AOM dissolved in normal saline at a dose of 15 mg/kg of body weight once per week for 2 weeks. All rats were necropsied 7 weeks after the last AOM injection, and the ACF were visualized under light microscopy in the formalin-fixed, unsectioned methylene blue-stained colons. The total number of aberrant crypt in Bif, Lac, and Bif+Lac groups were significantly lower than that of the AOM alone group and the percentage of inhibitions weas 35.0, 45.4 and 45.0%, respectively. Significant inhibition (p<0.001) in the total number of ACF was also observed in LAB treated groups (Bif, Lac, and Bif+Lac group by 30.3, 38.6, and 41.2%, respec-tively). Furthermore, cecal pH appeared to significantly decrease by LAB administration. The results of present study provide some evidences for potential colon tumor-inhibitory properties of lactic cultures and fermented dairy products.
- Effects of Lactic Acid Bacteria on Intestinal Microbial Enzyme Activity and Composition in Rats Treated with Azoxymethane
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Sang-Myeong Lee , Wan-Kyu Lee
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J. Microbiol. 2001;39(3):154-161.
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Abstract
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In recent years, colon cancer has been reported to be one of the most important causes of cancer morbidity and mortality in Korea. Epidemiological and experimental studies suggest that lactic acid bacteria (LAB) used to ferment dairy products inhibits colon carcinogenesis. The present study was designed to determine whether the colon cancer inhibitory effect of LAB (Bifidobacterium longum HY8001; Bif and Lactobacillus acidophilus HY2104; Lac) of Korean origin, is associated with intestinal microflora composition and certain enzyme activity in rats treated with azoxymethane (AOM). At five weeks of age, SD rats were divided at random into four (AOM alone, Bif, Lac, and Bif+Lac) groups. Oral administration of lactic acid bacteria cultures were performed daily until the termination of the study. Two weeks later, all animals were given a subcutaneous injection of AOM dissolved in normal saline at a dose of 15 mg/kg of body weight once weekly for 2 weeks. Every two weeks for 10 weeks, five of the rats in each group were randomly chosen for fecal specimen collection. The fecal specimens were used for assay of [beta]-glucuronidase and nitroreductase, and analysis of intestinal microflora composition. The activity of [beta]-glucuronidase which plays an important role in the production of the carcinogenic metabolite of azoxymethane was remarkably increased in the AOM alone group after AOM injection and maintained the high level during the experiment. However, LAB inhibited the AOM-induced increase in [beta]-glucuronidase activity. Nitroreductase activity decreased by 30-40% in LAB treated groups in comparison with that of the AOM alone group. The results of the present study suggest that LAB inhibits colon carcinogenesis by modulating the metabolic activity of intestinal microflora and improving the composition of intestinal microflora.