A Gram-positive, aerobic, non-motile, pale-yellow, and rodshaped
bacterium, designated as Gsoil 188T, was isolated from
the soil of a ginseng field in Pocheon, South Korea. A phylogenetic
analysis based on 16S rRNA gene sequence comparison
revealed that the strain formed a distinct lineage within
the genus Brevibacterium and was most closely related to B.
epidermidis NBRC 14811T (98.4%), B. sediminis FXJ8.269T
(98.2%), B. avium NCFB 3055T (98.1%), and B. oceani BBH7T
(98.1%), while it shared less than 98.1% identity with the other
species of this genus. The DNA G + C content was 68.1 mol%.
The predominant quinone was MK-8(H2). The major fatty
acids were anteiso-C15:0 and anteiso-C17:0. The cell wall peptidoglycan
of strain Gsoil 188T contained meso-diaminopimelic
acid. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol,
and an unidentified aminolipid. The
physiological and biochemical characteristics, low DNA-DNA
relatedness values, and taxonomic analysis allowed the differentiation
of strain Gsoil 188T from the other recognized
species of the genus Brevibacterium. Therefore, strain Gsoil
188T represents a novel species of the genus Brevibacterium,
for which the name Brevibacterium anseongense sp. nov. is
proposed, with the type strain Gsoil 188T (= KACC 19439T
= LMG 30331T).