Journal of Microbiology

Research Support, Non-U.S. Gov't

In Vivo Studies with a Candida tropicalis Isolate Exhibiting Paradoxical Growth In Vitro in the Presence of High Concentration of Caspofungin: Sedigh Bayegan , Laszlo Majoros , Gabor Kardos , Adam Kemény-Beke , Cecilia Miszti , Renato Kovacs , Rudolf Gesztelyi; J. Microbiol. 2010;48(2):170-173. Published online May 1, 2010; DOI: https://doi.org/10.1007/s12275-010-9221-y

57 View
0 Download
18 Crossref

We investigated the activity of caspofungin against a Candida tropicalis clinical isolate showing paradoxical growth in vitro. BALB/c mice immunosuppressed by cyclophosphamide were infected intraperitoneally using 107 CFU/mouse. Caspofungin was administered intraperitoneally once daily for 5 days or as a single dose using the following doses: 0.12, 0.25, 1, 2, 3, 5, and 15 mg/kg. The single dose of caspofungin was effective only at 5 and 15 mg/kg concentrations (100% survival). Five-day caspofungin treatment led to 100% survival at doses of 1 mg/kg or higher. Caspofungin treatment significantly decreased the number of viable yeasts in the peritoneal lavage samples as well as in the infected abscesses at doses 1, 3, 5, and 15 mg/kg caspofungin as compared to the untreated control (P<0.001 in all cases), and even to the group treated with 0.12 mg/kg caspofungin (P<0.05 in all cases). At 2 mg/kg caspofungin dose, sterilization of the internal organs was reproducibly incomplete, suggesting that the role of paradoxical growth in the late clinical failure cannot be excluded.

Citations

Citations to this article as recorded by

Adaptation of the emerging pathogenic yeast Candida auris to high caspofungin concentrations correlates with cell wall changes
Violeta Lara-Aguilar, Cristina Rueda, Irene García-Barbazán, Sarai Varona, Sara Monzón, Pilar Jiménez, Isabel Cuesta, Ángel Zaballos, Óscar Zaragoza
Virulence.2021; 12(1): 1400. CrossRef
Relative Frequency of Paradoxical Growth and Trailing Effect with Caspofungin, Micafungin, Anidulafungin, and the Novel Echinocandin Rezafungin against Candida Species
Zoltán Tóth, Lajos Forgács, Tamás Kardos, Renátó Kovács, Jeffrey B. Locke, Gábor Kardos, Fruzsina Nagy, Andrew M. Borman, Awid Adnan, László Majoros
Journal of Fungi.2020; 6(3): 136. CrossRef
Minimal Inhibitory Concentration (MIC)-Phenomena in Candida albicans and Their Impact on the Diagnosis of Antifungal Resistance
Ulrike Binder, Maria Aigner, Brigitte Risslegger, Caroline Hörtnagl, Cornelia Lass-Flörl, Michaela Lackner
Journal of Fungi.2019; 5(3): 83. CrossRef
Treatment of Invasive Candidiasis: A Narrative Review
Ronen Ben-Ami
Journal of Fungi.2018; 4(3): 97. CrossRef
Pharmacokinetics of extended dose intervals of micafungin in haematology patients: optimizing antifungal prophylaxis
E W Muilwijk, J A Maertens, W J F M van der Velden, R ter Heine, A Colbers, D M Burger, D Andes, K Theunissen, N M A Blijlevens, R J M Brüggemann
Journal of Antimicrobial Chemotherapy.2018; 73(11): 3095. CrossRef
An Update on Candida tropicalis Based on Basic and Clinical Approaches
Diana L. Zuza-Alves, Walicyranison P. Silva-Rocha, Guilherme M. Chaves
Frontiers in Microbiology.2017;[Epub] CrossRef
Recent Insights into the Paradoxical Effect of Echinocandins
Johannes Wagener, Veronika Loiko
Journal of Fungi.2017; 4(1): 5. CrossRef
Drug resistance mechanisms and their regulation in non-albicans Candidaspecies
Anna Kołaczkowska, Marcin Kołaczkowski
Journal of Antimicrobial Chemotherapy.2016; 71(6): 1438. CrossRef
The Eagle-Like Effect of the Echinocandins: Is It Relevant for Clinical Decisions?
Kayla R. Stover, John D. Cleary
Current Fungal Infection Reports.2015; 9(2): 88. CrossRef
Paradoxical Growth of Candida albicans in the Presence of Caspofungin Is Associated with Multiple Cell Wall Rearrangements and Decreased Virulence
Cristina Rueda, Manuel Cuenca-Estrella, Oscar Zaragoza
Antimicrobial Agents and Chemotherapy.2014; 58(2): 1071. CrossRef
The Eagle-like effect of echinocandins: what’s in a name?
Kim Vanstraelen, Katrien Lagrou, Johan Maertens, Joost Wauters, Ludo Willems, Isabel Spriet
Expert Review of Anti-infective Therapy.2013; 11(11): 1179. CrossRef
The Postantifungal and Paradoxical Effects of Echinocandins Against Candida spp.
Brad Moriyama, Stacey A Henning, Scott R Penzak, Thomas J Walsh
Future Microbiology.2012; 7(5): 565. CrossRef
Echinocandin Resistance in Candida Species: Mechanisms of Reduced Susceptibility and Therapeutic Approaches
Nicholas D Beyda, Russell E Lewis, Kevin W Garey
Annals of Pharmacotherapy.2012; 46(7-8): 1086. CrossRef
The potential impact of antifungal drug resistance mechanisms on the host immune response to Candida
Russell E. Lewis, Pierluigi Viale, Dimitrios P Kontoyiannis
Virulence.2012; 3(4): 368. CrossRef
Candida glabrata, Candida parapsilosisandCandida tropicalis: biology, epidemiology, pathogenicity and antifungal resistance
Sónia Silva, Melyssa Negri, Mariana Henriques, Rosário Oliveira, David W. Williams, Joana Azeredo
FEMS Microbiology Reviews.2012; 36(2): 288. CrossRef
Effect of nikkomycin Z and 50% human serum on the killing activity of high-concentration caspofungin againstCandida speciesusing time-kill methodology
Judit Szilágyi, Richárd Földi, Sedigh Bayegan, Gábor Kardos, László Majoros
Journal of Chemotherapy.2012; 24(1): 18. CrossRef
Echinocandins: Are They All The Same?
PK Mukherjee, D Sheehan, L Puzniak, H Schlamm, MA Ghannoum
Journal of Chemotherapy.2011; 23(6): 319. CrossRef
Paradoxical Effect of Caspofungin against Candida Bloodstream Isolates Is Mediated by Multiple Pathways but Eliminated in Human Serum
Ryan K. Shields, M. Hong Nguyen, Chen Du, Ellen Press, Shaoji Cheng, Cornelius J. Clancy
Antimicrobial Agents and Chemotherapy.2011; 55(6): 2641. CrossRef

Journal Article

Symbiotic Relationship between Microbacterium sp. SK0812 and Candida tropicalis SK090404: Seung Won Kang , Bo Young Jeon , Tae Sik Hwang , Doo Hyun Park; J. Microbiol. 2009;47(6):721-727. Published online February 4, 2010; DOI: https://doi.org/10.1007/s12275-009-0146-2

48 View
0 Download
4 Crossref

Abstract

A bacterium growing inside yeast cytoplasm was observed by light microscope without staining. The bacterium was separately stained from yeast cell by a fluorescent dye, 4′,6-diamidino-2-phenylindole (DAPI). The bacterium actively moved inside yeast cytoplasm and propagated in company with the yeast growth. The bacterium was separated from the yeast cytoplasm by selective disruption of yeast cells and the yeast without the intracellular bacterium (YWOB) was obtained by selective inactivation of bacterial cells. The yeast and the intracellular bacterium were identified as Candida tropicalis and Microbacterium sp., respectively. The length of Microbacterium sp. and C. tropicalis measured with SEM image was smaller than 0.5 μm and was larger than 5 μm, respectively. The yeast with the intracellular bacterium (YWIB) grew in a starch-based medium but the YWOB was not C. tropicalis has neither extracellular nor intracellular saccharification enzyme. Glucose was produced from starch by the extracellular crude enzyme (culture fluid) of Microbacterium sp. YWIB produced significantly more ethanol from glucose than YWOB but did not from starch. Conclusively, C. tropicalis is thought to catabolize starch dependent upon Microbacterium sp. growing in its cytoplasm and furnish stable habitat for the Microbacterium sp.

Citations

Citations to this article as recorded by

Tripartite interactions comprising yeast-endobacteria systems in the gut of vector mosquitoes
Alessia Cappelli, Claudia Damiani, Aida Capone, Jovana Bozic, Priscilla Mensah, Emanuela Clementi, Roberta Spaccapelo, Guido Favia, Irene Ricci
Frontiers in Microbiology.2023;[Epub] CrossRef
Uncovering the hidden bacterial ghost communities of yeast and experimental evidences demonstrates yeast as thriving hub for bacteria
B. Indu, Tallapragada Keertana, Sahu Ipsita, Uppada Jagadeeshwari, Chintalapati Sasikala, Chintalapati Venkata Ramana
Scientific Reports.2021;[Epub] CrossRef
Wolbachia pipientis grows in Saccharomyces cerevisiae evoking early death of the host and deregulation of mitochondrial metabolism
Cristina Uribe‐Alvarez, Natalia Chiquete‐Félix, Lilia Morales‐García, Arlette Bohórquez‐Hernández, Norma Laura Delgado‐Buenrostro, Luis Vaca, Antonio Peña, Salvador Uribe‐Carvajal
MicrobiologyOpen.2019;[Epub] CrossRef
Managing plant-environment-symbiont interactions to promote plant performance under low temperature stress
Omid Askari-Khorasgani, Harlene Hatterman-Valenti, Francisco Borja Flores, Mohammad Pessarakli
Journal of Plant Nutrition.2019; 42(16): 2010. CrossRef

First
Prev
Page of 1
Next
Last

Search