Journal Articles
- In vitro disinfection efficacy and clinical protective effects of common disinfectants against acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio isolates in Pacific white shrimp Penaeus vannamei
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Peizhuo Zou , Qian Yang , Hailiang Wang , Guosi Xie , Zhi Cao , Xing Chen , Wen Gao , Jie Huang
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J. Microbiol. 2020;58(8):675-686. Published online July 27, 2020
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DOI: https://doi.org/10.1007/s12275-020-9537-1
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Abstract
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Acute hepatopancreatic necrosis disease (AHPND) is one
of the most significant bacterial diseases in global shrimp
culture, causing severe economic losses. In the present study,
we carried out in vitro antimicrobial tests to investigate the
disinfection efficacy of 14 common disinfectants toward different
AHPND-causing Vibrio spp., including eight isolates
of V. parahaemolyticus, four isolates of V. campbellii, and
one isolate of V. owensii. Polyhexamethylene biguanidine hydrochloride
(PHMB) was revealed to possess the strongest
inhibitory activity. Through analyzing and evaluating the results
of antimicrobial tests and acute toxicity test, we selected
PHMB and hydrogen peroxide (H2O2) for further clinical
protection test. Clinical manifestations indicated that both
PHMB (2 mg/L and 4 mg/L) and H2O2 (12 mg/L) could effectively
protect juvenile Penaeus vannamei from the infection
of V. parahaemolyticus isolate Vp362 at 106 CFU/ml, and the
survival rate was over 80%. When the bacterial concentration
was reduced to 105 CFU/ml, 104 CFU/ml, and 103 CFU/ml,
the survival rate after treated by 1 mg/L PHMB was 64.44%,
93.33%, and 100%, respectively. According to the results,
PHMB and H2O2 showed a lower toxicity while a better protection
activity, particularly against a lower concentration of
the pathogens. Therefore, these two disinfectants are proved
to be promising disinfectants that can be applied to prevent
and control AHPND in shrimp culture. Moreover, the methods
of this study also provided valuable information for the
prevention of other important bacterial diseases and suggested
a reliable means for screening potential drugs in aquaculture.
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- Basigin binds bacteria and activates Dorsal signaling to promote antibacterial defense in Penaeus vannamei
Linwei Yang, Zi-ang Wang, Yushi Gan, Hongliang Zuo, Hengwei Deng, Shaoping Weng, Jianguo He, Xiaopeng Xu
Fish & Shellfish Immunology.2023; 142: 109123. CrossRef - Clinical protective effects of polyhexamethylene biguanide hydrochloride (PHMB) against Vibrio parahaemolyticus causing translucent post-larvae disease (VTPD) in Penaeus vannamei
Tianchang Jia, Tingting Xu, Jitao Xia, Shuang Liu, Wenqiang Li, Ruidong Xu, Jie Kong, Qingli Zhang
Journal of Invertebrate Pathology.2023; 201: 108002. CrossRef
- Mutation of the cyclic di-GMP phosphodiesterase gene in Burkholderia lata SK875 attenuates virulence and enhances biofilm formation
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Hae-In Jung , Yun-Jung Kim , Yun-Jung Lee , Hee-Soo Lee , Jung-Kee Lee , Soo-Ki Kim
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J. Microbiol. 2017;55(10):800-808. Published online September 28, 2017
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DOI: https://doi.org/10.1007/s12275-017-7374-7
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Abstract
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Burkholderia sp. is a gram-negative bacterium that commonly
exists in the environment, and can cause diseases in plants,
animals, and humans. Here, a transposon mutant library of
a Burkholderia lata isolate from a pig with swine respiratory
disease in Korea was screened for strains showing attenuated
virulence in Caenorhabditis elegans. One such mutant was
obtained, and the Tn5 insertion junction was mapped to
rpfR, a gene encoding a cyclic di-GMP phosphodiesterase that
functions as a receptor. Mutation of rpfR caused a reduction
in growth on CPG agar and swimming motility as well as a
rough colony morphology on Congo red agar. TLC analysis
showed reduced AHL secretion, which was in agreement with
the results from plate-based and bioluminescence assays. The
mutant strain produced significantly more biofilm detected
by crystal violet staining than the parent strain. SEM of the
mutant strain clearly showed that the overproduced biofilm
contained a filamentous structure. These results suggest
that the cyclic di-GMP phosphodiesterase RpfR plays an important
role in quorum sensing modulation of the bacterial
virulence and biofilm formation.
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Citations
Citations to this article as recorded by

- Biocontrol of bacterial wilt disease in tomato using Bacillus subtilis strain R31
Yunhao Sun, Yutong Su, Zhen Meng, Jie Zhang, Li Zheng, Shuang Miao, Di Qin, Yulan Ruan, Yanhui Wu, Lina Xiong, Xun Yan, Zhangyong Dong, Ping Cheng, Mingwei Shao, Guohui Yu
Frontiers in Microbiology.2023;[Epub] CrossRef - Comprehensive genome analysis of Burkholderia contaminans SK875, a quorum-sensing strain isolated from the swine
Eiseul Kim, Hae-In Jung, Si Hong Park, Hae-Yeong Kim, Soo-Ki Kim
AMB Express.2023;[Epub] CrossRef - Comparative genomics and transcriptomic response to root exudates of six rice root-associated Burkholderia sensu lato species
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Peer Community Journal.2023;[Epub] CrossRef -
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Mingfang Wang, Xia Li, Shihao Song, Chaoyu Cui, Lian-Hui Zhang, Yinyue Deng, Gladys Alexandre
Applied and Environmental Microbiology.2022;[Epub] CrossRef - A c-di-GMP Signaling Cascade Controls Motility, Biofilm Formation, and Virulence in Burkholderia thailandensis
Zhuo Wang, Xiaorong Xie, Daohan Shang, Laigong Xie, Yueyue Hua, Li Song, Yantao Yang, Yao Wang, Xihui Shen, Lei Zhang, Gladys Alexandre
Applied and Environmental Microbiology.2022;[Epub] CrossRef - Methodological tools to study species of the genus Burkholderia
Viola Camilla Scoffone, Gabriele Trespidi, Giulia Barbieri, Samuele Irudal, Aygun Israyilova, Silvia Buroni
Applied Microbiology and Biotechnology.2021; 105(24): 9019. CrossRef - Complete Genome Sequence of Burkholderia contaminans SK875, Isolated from the Respiratory Tract of a Pig in the Republic of Korea
Hae-In Jung, Sang-Won Lee, Soo-Ki Kim, Irene L. G. Newton
Microbiology Resource Announcements.2020;[Epub] CrossRef - Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia
Anja M. Richter, Mustafa Fazli, Nadine Schmid, Rebecca Shilling, Angela Suppiger, Michael Givskov, Leo Eberl, Tim Tolker-Nielsen
Frontiers in Microbiology.2019;[Epub] CrossRef - In silico comparative analysis of GGDEF and EAL domain signaling proteins from the Azospirillum genomes
Alberto Ramírez Mata, César Millán Pacheco, José F. Cruz Pérez, Martha Minjárez Sáenz, Beatriz E. Baca
BMC Microbiology.2018;[Epub] CrossRef
Review
- REVIEW] Enterococcus Infection Biology: Lessons from Invertebrate Host Models
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Grace J. Yuen , Frederick M. Ausubel
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J. Microbiol. 2014;52(3):200-210. Published online March 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-4011-6
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Abstract
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The enterococci are commensals of the gastrointestinal tract of many metazoans, from insects to humans. While they normally do not cause disease in the intestine, they can become pathogenic when they infect sites outside of the gut. Recently, the enterococci have become important nosocomial pathogens, with the majority of human enterococcal infections caused by two species, Enterococcus faecalis and Enterococcus faecium. Studies using invertebrate infection models have revealed insights into the biology of enterococcal infections, as well as general principles underlying host innate immune defense. This review highlights recent findings on Enterococcus infection biology from two invertebrate infection models, the greater wax moth Galleria mellonella and the free-living bacteriovorous nematode Caenorhabditis elegans.
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Citations
Citations to this article as recorded by

- Microbiological profiles and clinical outcomes of critically ill surgical patients with lower gastrointestinal perforation in Japan: a single-center retrospective observational study
Takashi Nishikawa, Jiro Ito, Ryutaro Seo, Koichi Ariyoshi, Hiroyuki Mima
BMC Anesthesiology.2024;[Epub] CrossRef - Navigating ESKAPE Pathogens: Considerations and Caveats for Animal Infection Models Development
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Julián Cerón
Disease Models & Mechanisms.2023;[Epub] CrossRef - Enterococcus casseliflavus Infection: A Review of Clinical Features and Treatment
Yusuke Yoshino
Infection and Drug Resistance.2023; Volume 16: 363. CrossRef - It's all relative: analyzing microbiome compositions, its significance, pathogenesis and microbiota derived biofilms: Challenges and opportunities for disease intervention
Sahar Zaidi, Khursheed Ali, Asad U. Khan
Archives of Microbiology.2023;[Epub] CrossRef - Antimicrobial Activity of Ceragenins against Vancomycin-Susceptible and -Resistant Enterococcus spp.
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Gene Cluster-Carrying Linear Plasmid
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mSphere.2020;[Epub] CrossRef - The red flour beetle Tribolium castaneum: A model for host-microbiome interactions
Aparna Agarwal, Deepa Agashe, Jeffrey P. Demuth
PLOS ONE.2020; 15(10): e0239051. CrossRef - RNA-Seq comparative analysis reveals the response of Enterococcus faecalis TV4 under fluoride exposure
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Research Support, Non-U.S. Gov't
- Lactobacillus salivarius Strain FDB89 Induced Longevity in Caenorhabditis elegans by Dietary Restriction
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Yang Zhao , Liang Zhao , Xiaonan Zheng , Tianjiao Fu , Huiyuan Guo , Fazheng Ren
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J. Microbiol. 2013;51(2):183-188. Published online April 27, 2013
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DOI: https://doi.org/10.1007/s12275-013-2076-2
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Abstract
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In this study, we utilized the nematode Caenorhabditis elegans to assess potential life-expanding effect of Lactobacillus salivarius strain FDB89 (FDB89) isolated from feces of centenarians in Bama County (Guangxi, China). This study showed that feeding FDB89 extended the mean life span in C. elegans by up to 11.9% compared to that of control nematodes. The reduced reproductive capacities, pharyngeal pumping rate, growth, and increased superoxide dismutase (SOD) activity and XTT reduction capacity were also observed in FDB89 feeding worms. To probe the anti-aging mechanism further, we incorporated a food gradient feeding assay and assayed the life span of eat-2 mutant. The results demonstrated that the maximal life span of C. elegans fed on FDB89 was achieved at the concentration of 1.0 mg bacterial cells/plate, which was 10-fold greater than that of C. elegans fed on E. coli OP50 (0.1 mg bacterial cells/plate). However, feeding FDB89 could not further extend the life span of eat-2 mutant. These results indicated that FDB89 modulated the longevity of C. elegans in a dietary restriction-dependent manner and expanded the understanding of anti-aging effect of probiotics.