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- Cloning, Expression, and Characterization of Xylose Reductase with Higher Activity from Candida tropicalis
- Feiwei Zhang , Dairong Qiao , Hui Xu , Chong Liao , Shilin Li , Yi Cao
- J. Microbiol. 2009;47(3):351-357. Published online June 26, 2009
- DOI: https://doi.org/10.1007/s12275-008-0225-9
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- 17 Crossref
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Abstract
- Xylose reductase (XR) is a key enzyme in xylose metabolism because it catalyzes the reduction of xylose to xylitol. In order to study the characteristics of XR from Candida tropicalis SCTCC 300249, its XR gene(xyl1) was cloned and expressed in Escherichia coli BL21 (DE3). The fusion protein was purified effectively by Ni2+-chelating chromatography, and the kinetics of the recombinant XR was investigated. The Km values of the C. tropicalis XR for NADPH and NADH were 45.5 uM and 161.9 uM, respectively, which demonstrated that this XR had dual coenzyme specificity. Moreover, this XR showed the highest catalytic efficiency (kcat=1.44x04 min-1) for xylose among the characterized aldose reductases. Batch fermentation was performed with Saccharomyces serivisiae W303-1A:pYES2XR, and resulted in 7.63 g/L cell mass, 93.67 g/L xylitol, and 2.34 g/Lh xylitol productivity. This XR coupled with its dual coenzyme specificity, high activity, and catalytic efficiency proved its utility in in vitro xylitol production.
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Citations
Citations to this article as recorded by
- Efficient production of ginsenoside Rh2 from xylose by remodeling metabolism in Saccharomyces cerevisiae
Wanze Zhang, Jiale Zhang, Xiaomeng Zhao, Zhanwei Zhang, Shifan He, Xueke Bian, Haibin Wang, Chuanbo Zhang, Wenyu Lu
Chemical Engineering Journal.2024; 494: 153120. CrossRef - Recent insights, applications and prospects of xylose reductase: a futuristic enzyme for xylitol production
Yogita Lugani, Munish Puri, Balwinder Singh Sooch
European Food Research and Technology.2021; 247(4): 921. CrossRef - Characterization of d-xylose reductase, XyrB, from Aspergillus niger
Agata Terebieniec, Tania Chroumpi, Adiphol Dilokpimol, Maria Victoria Aguilar-Pontes, Miia R. Mäkelä, Ronald P. de Vries
Biotechnology Reports.2021; 30: e00610. CrossRef - Combining Xylose Reductase from Spathaspora arborariae with Xylitol Dehydrogenase from Spathaspora passalidarum to Promote Xylose Consumption and Fermentation into Xylitol by Saccharomyces cerevisiae
Adriane Mouro, Angela A. dos Santos, Denis D. Agnolo, Gabriela F. Gubert, Elba P. S. Bon, Carlos A. Rosa, César Fonseca, Boris U. Stambuk
Fermentation.2020; 6(3): 72. CrossRef - Kinetics and Predicted Structure of a Novel Xylose Reductase from Chaetomium thermophilum
Julian Quehenberger, Tom Reichenbach, Niklas Baumann, Lukas Rettenbacher, Christina Divne, Oliver Spadiut
International Journal of Molecular Sciences.2019; 20(1): 185. CrossRef - Biosynthetic strategies to produce xylitol: an economical venture
Yirong Xu, Ping Chi, Muhammad Bilal, Hairong Cheng
Applied Microbiology and Biotechnology.2019; 103(13): 5143. CrossRef - A halotolerant aldose reductase from Debaryomyces nepalensis: gene isolation, overexpression and biochemical characterization
Bhaskar Paidimuddala, Gopala Krishna Aradhyam, Sathyanarayana N. Gummadi
RSC Advances.2017; 7(33): 20384. CrossRef - Inhibition of Debaryomyces nepalensis xylose reductase by lignocellulose derived by-products
Bhaskar Paidimuddala, Ashish Rathod, Sathyanarayana N. Gummadi
Biochemical Engineering Journal.2017; 121: 73. CrossRef - Bioprospecting and evolving alternative xylose and arabinose pathway enzymes for use in Saccharomyces cerevisiae
Sun-Mi Lee, Taylor Jellison, Hal S. Alper
Applied Microbiology and Biotechnology.2016; 100(5): 2487. CrossRef - The yeast Scheffersomyces amazonensis is an efficient xylitol producer
Raquel M. Cadete, Monaliza A. Melo-Cheab, Adriana L. Viana, Evelyn S. Oliveira, César Fonseca, Carlos A. Rosa
World Journal of Microbiology and Biotechnology.2016;[Epub] CrossRef - Identification and characterization of d-arabinose reductase and d-arabinose transporters from Pichia stipitis
Seiya Watanabe, Yuki Utsumi, Shigeki Sawayama, Yasuo Watanabe
Bioscience, Biotechnology, and Biochemistry.2016; 80(11): 2151. CrossRef - Sequence analysis of KmXYL1 genes and verification of thermotolerant enzymatic activities of xylose reductase from four Kluyveromyces marxianus strains
Jae-Bum Park, Jin-Seong Kim, Seung-Won Jang, Deok-Ho Kweon, Eock Kee Hong, Won Cheol Shin, Suk-Jin Ha
Biotechnology and Bioprocess Engineering.2016; 21(5): 581. CrossRef - Cloning, expression, and characterization of a novel xylose reductase from Rhizopus oryzae
Min Zhang, Shao‐tong Jiang, Zhi Zheng, Xing‐jiang Li, Shui‐zhong Luo, Xue‐feng Wu
Journal of Basic Microbiology.2015; 55(7): 907. CrossRef - Metabolic engineering strategies for improving xylitol production from hemicellulosic sugars
Buli Su, Mianbin Wu, Jianping Lin, Lirong Yang
Biotechnology Letters.2013; 35(11): 1781. CrossRef - Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777
Biao Zhang, Ling Zhang, Dongmei Wang, Xiaolian Gao, Jiong Hong
Journal of Industrial Microbiology & Biotechnology.2011; 38(12): 2001. CrossRef - Purification and biochemical characterization of a moderately halotolerant NADPH dependent xylose reductase from Debaryomyces nepalensis NCYC 3413
Sawan Kumar, Sathyanarayana N. Gummadi
Bioresource Technology.2011; 102(20): 9710. CrossRef - Cosubstrate effect on xylose reductase and xylitol dehydrogenase activity levels, and its consequence on xylitol production by Candida tropicalis
Elena Tamburini, Ercolina Bianchini, Alessandro Bruni, Giuseppe Forlani
Enzyme and Microbial Technology.2010; 46(5): 352. CrossRef
- Efficient production of ginsenoside Rh2 from xylose by remodeling metabolism in Saccharomyces cerevisiae
- Strain Improvement of Candida tropicalis for the Production of Xylitol:Biochemical and Physiological Characterization of Wild-type and Mutant Strain CT-OMV5
- Ravella Sreenivas Rao , Cherukuri Pavana Jyothi , Reddy Shetty Prakasham , Chaganti Subba Rao , Ponnupalli Nageshwara Sarma , Linga Venkateswar Rao
- J. Microbiol. 2006;44(1):113-120.
- DOI: https://doi.org/2328 [pii]
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Abstract
- Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamydospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5.
Journal Article
- Molecular Investigation of Two Consecutive Nosocomial Clusters of Candida tropicalis Candiduria Using Pulsed-Field Gel Electrophoresis
- Joon Rho , Jong Hee Shin , Jeong Won Song , Mi-Ra Park , Seung Jung Kee , Sook Jin Jang , Young Kyu Park , Soon Pal Suh , Dong Wook Ryang
- J. Microbiol. 2004;42(2):80-86.
- DOI: https://doi.org/2041 [pii]
- 34 View
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Abstract
- Pulsed-field gel electrophoresis (PFGE) typing was applied to the epidemiological investigation of 21 Candida tropicalis isolates collected from urine specimens of 11 patients and one healthcare worker, in an intensive care unit (ICU) over a 4-month period. Seventeen epidemiologically unrelated strains from 14 patients were also tested to determine the discriminatory power of PFGE. PFGE typing consisted of electrophoretic karyotyping (EK) and restriction endonuclease analysis of genomic DNA (REAG), using two restriction enzymes (BssHII and SfiI). The EK pattern was the same in all 38 isolates, while REAG using SfiI separated the isolates into nine types. However, 16 different PFGE types were identified by REAG with BssHII, and the same results were obtained when the results of both REAG tests were combined. In serial urinary isolates from 10 patients, all strains from each patient had the same PFGE pattern. While the epidemiologically unrelated strains from 14 patients consisted of 13 different PFGE types, the 20 isolates from the 11 ICU patients fell into only two PFGE types (types C1 and C2), and these apparently originated from the two different outbreaks. All strains of type C1 (n = 12) were isolated from six patients, between November 1999 and January 2000, and all of the type C2 strains (n=8) were isolated from five patients, during January and February 2000. This study shows two consecutive clusters of C. tropicalis candiduria in an ICU, defined by PFGE typing, and also demonstrates that a PFGE typing method using BssHII is perhaps the most useful method for investigating C. tropicalis candiduria.
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