Journal of Microbiology

Journal Articles

Phylogenetic Assessment of Understudied Families in Hymenochaetales (Basidiomycota, Fungi)-Reporting Uncovered Species and Reflecting the Recent Taxonomic Updates in the Republic of Korea: Yoonhee Cho, Dohye Kim, Young Woon Lim; J. Microbiol. 2024;62(6):429-447. Published online May 16, 2024; DOI: https://doi.org/10.1007/s12275-024-00120-5

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Abstract: Hymenochaetales Oberw. is an order classified in Basidiomycota of Fungi, and species in this order display notable diversity. They exhibit various fruiting body shapes, including clavarioid, effused-reflexed, and resupinate basidiomes. Few mycorrhizal species have been reported in Hymenochaetales, but wood-decaying species dominate the order. Hymenochaetaceae Imazeki & Toki and Schizoporaceae Jülich are the most species-rich families within Hymenochaetales, and most species in the Republic of Korea belong to these two families. As such, current taxonomic classification and nomenclature are not reflected upon species in the remaining Hymenochaetales families. For this study, a multifaceted morphological and multigenetic marker-based phylogenetic investigation was conducted to, firstly, comprehensively identify understudied Hymenochaetales specimens in Korea and, secondly, reflect the updates on the species classification. Five genetic markers were assessed for the phylogenetic analysis: nuclear small subunit ribosomal DNA (nSSU), internal transcribed spacer (ITS), nuclear large subunit ribosomal DNA (nLSU), RNA polymerase II subunit 2 gene (RPB2), and translation elongation factor 1 gene (TEF1). The results from phylogenetic analysis supported 18 species classified under eight families (excluding Hymenochaetaceae and Schizoporaceae) in Korea. Species formerly placed in Rickenellaceae and Trichaptum sensu lato have been systematically revised based on recent taxonomic reconstructions. In addition, our findings revealed one new species, Rickenella umbelliformis, and identified five formerly nationally unreported species classified under five understudied families. Our findings contribute to a better understanding of Hymenochaetales diversity and highlight the need for continued research.

Transcriptome‑based Mining of the Constitutive Promoters for Tuning Gene Expression in Aspergillus oryzae: Kobkul Laoteng , Jutamas Anantayanon , Chanikul Chutrakul , Sarocha Panchanawaporn , Sukanya Jeennor; J. Microbiol. 2023;61(2):199-210. Published online February 6, 2023; DOI: https://doi.org/10.1007/s12275-023-00020-0

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Transcriptional regulation has been adopted for developing metabolic engineering tools. The regulatory promoter is a crucial genetic element for strain optimization. In this study, a gene set of Aspergillus oryzae with highly constitutive expression across different growth stages was identified through transcriptome data analysis. The candidate promoters were functionally characterized in A. oryzae by transcriptional control of β-glucuronidase (GUS) as a reporter. The results showed that the glyceraldehyde triphosphate dehydrogenase promoter (PgpdA1) of A. oryzae with a unique structure displayed the most robust strength in constitutively controlling the expression compared to the PgpdA2 and other putative promoters tested. In addition, the ubiquitin promoter (Pubi) of A. oryzae exhibited a moderate expression strength. The deletion analysis revealed that the 5' untranslated regions of gpdA1 and ubi with the length of 1028 and 811 nucleotides, counted from the putative translation start site (ATG), respectively, could efficiently drive the GUS expression. Interestingly, both promoters could function on various carbon sources for cell growth. Glucose was the best fermentable carbon source for allocating high constitutive expressions during cell growth, and the high concentrations (6–8% glucose, w/v) did not repress their functions. It was also demonstrated that the secondary metabolite gene coding for indigoidine could express under the control of PgpdA1 or Pubi promoter. These strong and moderate promoters of A. oryzae provided beneficial options in tuning the transcriptional expression for leveraging the metabolic control towards the targeted products.

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Construction of an Aspergillus oryzae △nptB△pyrG Host for Homologous Expression of Lipase and Catalytic Property Characterization of Recombinant Lipase
Yueting Zhang, Hongmei Nie, Fei Zhang, Mengmeng Jin, Zhao Wang, Jianyong Zheng
Applied Biochemistry and Biotechnology.2024;[Epub] CrossRef
Mining and Understanding of New Transcriptional Regulatory Elements from Licorice-Derived Endophyte Serratia Rubidaea W12-1
Ying Zhang, Yunyang Ma, Bing Hu, H.M. Zabed, A.K. Singh, M.A. Ibrahim, N. Chen
BIO Web of Conferences.2024; 142: 03018. CrossRef
Exploring and Engineering Novel Strong Promoters for High-Level Protein Expression in Bacillus subtilis DB104 through Transcriptome Analysis
Ji-Su Jun, Hyang-Eun Jeong, Kwang-Won Hong
Microorganisms.2023; 11(12): 2929. CrossRef
Efficient de novo production of bioactive cordycepin by Aspergillus oryzae using a food-grade expression platform
Sukanya Jeennor, Jutamas Anantayanon, Sarocha Panchanawaporn, Chanikul Chutrakul, Wanwipa Vongsangnak, Kobkul Laoteng
Microbial Cell Factories.2023;[Epub] CrossRef

Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains: Amanda Aparecida Seribelli , Tamara R. Machado Ribeiro , Patrick da Silva† , Isabela Mancini Martins , Felipe Pinheiro Vilela , Marta I. Cazentini Medeiros , Kamila Chagas Peronni , Wilson Araújo da Silva Junior , Cristiano Gallina Moreira , Juliana Pfrimer Falcão; J. Microbiol. 2021;59(9):861-870. Published online August 12, 2021; DOI: https://doi.org/10.1007/s12275-021-1082-z

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Abstract

Salmonella Typhimurium (ST313) has caused an epidemic of invasive disease in sub-Saharan Africa and has been recently identified in Brazil. As the virulence of this ST is poorly understood, the present study aimed to (i) perform the RNAseq in vitro of S. Typhimurium STm30 (ST313) grown in Luria-Bertani medium at 37°C; (ii) compare it with the RNAseq of the S. Typhimurium SL1344 (ST19) and S. Typhimurium STm11 (ST19) strains under the same growing conditions; and (iii) examine the colonization capacity and expression of virulence genes and cytokines in murine colon. The STm30 (ST313) strain exhibited stronger virulence and was associated with a more inflammatory profile than the strains SL1344 (ST19) and STm11 (ST19), as demonstrated by transcriptome and in vivo assay. The expression levels of the hilA, sopD2, pipB, and ssaS virulence genes, other Salmonella pathogenicity islands SPI-1 and SPI-2 genes or effectors, and genes of the cytokines IL-1β, IFN-γ, TNF-α, IL-6, IL-17, IL-22, and IL-12 were increased during ST313 infection in C57BL/6J mice. In conclusion, S. Typhimurium STm30 (ST313) isolated from human feces in Brazil express higher levels of pathogenesis- related genes at 37°C and has stronger colonization and invasion capacity in murine colon due to its high expression levels of virulence genes, when compared with the S. Typhimurium SL1344 (ST19) and STm11 (ST19) strains. STm30 (ST313) also induces stronger expression of pro-inflammatory cytokines in this organ, suggesting that it causes more extensive tissue damage.

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Whole-Genome Sequencing Reveals the Population Structure and Genetic Diversity of Salmonella Typhimurium ST34 and ST19 Lineages
Zhen-xu Zhuo, Yu-lian Feng, Xi-wei Zhang, Hao Liu, Fang-yin Zeng, Xiao-yan Li
Journal of Microbiology.2024; 62(10): 859. CrossRef
Incremental increases in physiological fluid shear progressively alter pathogenic phenotypes and gene expression in multidrug resistant Salmonella
Jiseon Yang, Jennifer Barrila, Eric A. Nauman, Seth D. Nydam, Shanshan Yang, Jin Park, Ami D. Gutierrez-Jensen, Christian L. Castro, C. Mark Ott, Kristina Buss, Jason Steel, Anne D. Zakrajsek, Mary M. Schuff, Cheryl A. Nickerson
Gut Microbes.2024;[Epub] CrossRef
Virulence potential of Salmonella 1,4, [5],12:i:- strains isolated during decades from different sources in the Southeast region of Brazil
Giovana do Nascimento Pereira, Amanda Aparecida Seribelli, Carolina Nogueira Gomes, Felipe Pinheiro Vilela, Ludmilla Tonani, Monique Ribeiro Tiba-Casas, Marta Inês Cazentini Medeiros, Dália dos Prazeres Rodrigues, Márcia Regina von Zeska Kress, Juliana Pf
Brazilian Journal of Microbiology.2023; 54(4): 2827. CrossRef
Invasive non-typhoidal Salmonella (iNTS) aminoglycoside-resistant ST313 isolates feature unique pathogenic mechanisms to reach the bloodstream
Isabela Mancini Martins, Amanda Aparecida Seribelli, Tamara R. Machado Ribeiro, Patrick da Silva, Bruna Cardinali Lustri, Rodrigo T. Hernandes, Juliana Pfrimer Falcão, Cristiano Gallina Moreira
Infection, Genetics and Evolution.2023; 116: 105519. CrossRef
Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef
Antimicrobial resistance and genetic background of non-typhoidal Salmonella enterica strains isolated from human infections in São Paulo, Brazil (2000–2019)
Aline Parolin Calarga, Marco Tulio Pardini Gontijo, Luiz Gonzaga Paula de Almeida, Ana Tereza Ribeiro de Vasconcelos, Leandro Costa Nascimento, Taíse Marongio Cotrim de Moraes Barbosa, Thalita Mara de Carvalho Perri, Silvia Regina dos Santos, Monique Ribe
Brazilian Journal of Microbiology.2022; 53(3): 1249. CrossRef

Effect of biostimulation and bioaugmentation on hydrocarbon degradation and detoxification of diesel-contaminated soil: a microcosm study: Patricia Giovanella , Lídia de Azevedo Duarte , Daniela Mayumi Kita , Valéria Maia de Oliveira , Lara Durães Sette; J. Microbiol. 2021;59(7):634-643. Published online May 15, 2021; DOI: https://doi.org/10.1007/s12275-021-0395-2

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Abstract

Soil contamination with diesel oil is quite common during processes of transport and storage. Bioremediation is considered a safe, economical, and environmentally friendly approach for contaminated soil treatment. In this context, studies using hydrocarbon bioremediation have focused on total petroleum hydrocarbon (TPH) analysis to assess process effectiveness, while ecotoxicity has been neglected. Thus, this study aimed to select a microbial consortium capable of detoxifying diesel oil and apply this consortium to the bioremediation of soil contaminated with this environmental pollutant through different bioremediation approaches. Gas chromatography (GC-FID) was used to analyze diesel oil degradation, while ecotoxicological bioassays with the bioindicators Artemia sp., Aliivibrio fischeri (Microtox), and Cucumis sativus were used to assess detoxification. After 90 days of bioremediation, we found that the biostimulation and biostimulation/ bioaugmentation approaches showed higher rates of diesel oil degradation in relation to natural attenuation (41.9 and 26.7%, respectively). Phytotoxicity increased in the biostimulation and biostimulation/bioaugmentation treatments during the degradation process, whereas in the Microtox test, the toxicity was the same in these treatments as that in the natural attenuation treatment. In both the phytotoxicity and Microtox tests, bioaugmentation treatment showed lower toxicity. However, compared with natural attenuation, this approach did not show satisfactory hydrocarbon degradation. Based on the microcosm experiments results, we conclude that a broader analysis of the success of bioremediation requires the performance of toxicity bioassays.

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Heavy fuel oil-contaminated soil remediation by individual and bioaugmentation-assisted phytoremediation with Medicago sativa and with cold plasma-treated M. sativa
Jūratė Žaltauskaitė, Rimas Meištininkas, Austra Dikšaitytė, Laima Degutytė-Fomins, Vida Mildažienė, Zita Naučienė, Rasa Žūkienė, Kazunori Koga
Environmental Science and Pollution Research.2024; 31(20): 30026. CrossRef
Soil Corrosivity Under Natural Attenuation
Larissa O. da Silva, Sara H. de Oliveira, Rafael G. C. da Silva, Magda R. S. Vieira, Ivanilda R. de Melo, Severino L. Urtiga Filho
Materials Research.2024;[Epub] CrossRef
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Daniel Hidalgo-Lasso, Karina García-Villacís, Jeaneth Urvina Ulloa, Darwin Marín Tapia, Patricio Gómez Ortega, Frederic Coulon
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Oil biodegradation studies with an immobilized bacterial consortium in plant biomass for the construction of bench-scale bioreactor
Rachel M. Ferreira, Bernardo D. Ribeiro, Danielle.M.A. Stapelfeldt, Rodrigo P. do Nascimento, Maria de.F.R. Moreira
Cleaner Chemical Engineering.2023; 6: 100107. CrossRef
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Kai Zhang, Meng Liu, Xinlong Song, Dongyu Wang
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Functional analysis of Mpk1-mediated cell wall integrity signaling pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha: Hyunah Kim , Eun Jung Thak , Ji Yoon Yeon , Min Jeong Sohn , Jin Ho Choo , Jeong-Yoon Kim , Hyun Ah Kang; J. Microbiol. 2018;56(1):72-82. Published online January 4, 2018; DOI: https://doi.org/10.1007/s12275-018-7508-6

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Abstract

Understanding the characteristics and regulation mechanisms of cell wall integrity (CWI) in yeast is important not only for basic research but also in biotechnological applications. We found significantly different CWIs in two representative strains of the thermotolerant methylotrophic yeast Hansenula polymorpha. Compared to the A16 strain (classified as Ogataea polymorpha), the DL1-L strain (classified as Ogataea parapolymorpha) has a thinner cell wall that was found to be more fragile following long-term cultivation and more sensitive to zymolyase. To gain a deeper insight into this difference, we compared the characteristics of the Mpk1pmediated CWI signaling pathway in the two strains. While a DL1-L mutant deficient in Mpk1p (mpk1Δ) showed severe growth retardation at both normal and high growth temperatures and in the presence of cell-wall disrupting agents, the A16 mpk1Δ mutant displayed only a mild defect in cell growth. Sorbitol effect on rescuing growth retardation was different in the two mpk1Δ strains, which could partly be ascribed to subtle differences in the activation of HOG pathway. Among the cell wall disruptors evaluated, only caffeine clearly increased phosphorylation of Mpk1p in DL1-L, but not in A16. A transcriptome analysis of the DL1-L strain revealed that caffeine significantly increased the expression of a subset of cell-wall related genes in an Mpk1p-dependent manner, but not the expected Rlm1-target genes. Taken together, our data support an essential role for Mpk1p in maintaining CWI in H. polymorpha, although the requirement for Mpk1p and its regulation under diverse stress conditions varies depending on the strain background.

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Optimization, characterization, comparison of self-assembly VLP of capsid protein L1 in yeast and reverse vaccinology design against human papillomavirus type 52
Moh Egy Rahman Firdaus, Apon Zaenal Mustopa, Nurlaili Ekawati, Sheila Chairunnisa, Rosyida Khusniatul Arifah, Ai Hertati, Shasmita Irawan, Anika Prastyowati, Arizah Kusumawati, Maritsa Nurfatwa
Journal of Genetic Engineering and Biotechnology.2023; 21(1): 68. CrossRef
Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici
Tong-tong Zhu, Sheng-han Xiang, Lei Yang, Fang Tang, Wei Li, Ying-bao Liu, Wen-xiu Sun
European Journal of Plant Pathology.2022; 164(4): 525. CrossRef
The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts
Ricardo A. Ribeiro, Nuno Bourbon-Melo, Isabel Sá-Correia
Frontiers in Microbiology.2022;[Epub] CrossRef
The damage and tolerance mechanisms of Phaffia rhodozyma mutant strain MK19 grown at 28 °C
Li-Li Miao, Shuang Chi, Ting-Ting Hou, Zhi-Pei Liu, Ying Li
Microbial Cell Factories.2021;[Epub] CrossRef
Molecular characterization of Hsf1 as a master regulator of heat shock response in the thermotolerant methylotrophic yeast Ogataea parapolymorpha
Jin Ho Choo, Su-Bin Lee, Hye Yun Moon, Kun Hwa Lee, Su Jin Yoo, Keun Pil Kim, Hyun Ah Kang
Journal of Microbiology.2021; 59(2): 151. CrossRef
Pleiotropic Effects of Caffeine Leading to Chromosome Instability and Cytotoxicity in Eukaryotic Microorganisms
Woo-Hyun Chung
Journal of Microbiology and Biotechnology.2021; 31(2): 171. CrossRef
Short bZIP homologue of sulfur regulator Met4 from Ogataea parapolymorpha does not depend on DNA‐binding cofactors for activating genes in sulfur starvation
Su Jin Yoo, Min Jeong Sohn, Da Min Jeong, Hyun Ah Kang
Environmental Microbiology.2020; 22(1): 310. CrossRef
PlMAPK10, a Mitogen-Activated Protein Kinase (MAPK) in Peronophythora litchii, Is Required for Mycelial Growth, Sporulation, Laccase Activity, and Plant Infection
Liqun Jiang, Junjian Situ, Yi Zhen Deng, Lang Wan, Dandan Xu, Yubin Chen, Pinggen Xi, Zide Jiang
Frontiers in Microbiology.2018;[Epub] CrossRef

Alteration in the ultrastructural morphology of mycelial hyphae and the dynamics of transcriptional activity of lytic enzyme genes during basidiomycete morphogenesis: Elena Vetchinkina , Maria Kupryashina , Vladimir Gorshkov , Marina Ageeva , Yuri Gogolev , Valentina Nikitina; J. Microbiol. 2017;55(4):280-288. Published online January 26, 2017; DOI: https://doi.org/10.1007/s12275-017-6320-z

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Abstract

The morphogenesis of macromycetes is a complex multilevel process resulting in a set of molecular-genetic, physiological- biochemical, and morphological-ultrastructural changes in the cells. When the xylotrophic basidiomycetes Lentinus edodes, Grifola frondosa, and Ganoderma lucidum were grown on wood waste as the substrate, the ultrastructural morphology of the mycelial hyphal cell walls differed considerably between mycelium and morphostructures. As the macromycetes passed from vegetative to generative development, the expression of the tyr1, tyr2, chi1, chi2, exg1, exg2, and exg3 genes was acti-vated. These genes encode enzymes such as tyrosinase, chi-tinase, and glucanase, which play essential roles in cell wall growth and morphogenesis.

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Transcriptome analysis provides insight into gamma irradiation delaying quality deterioration of postharvest Lentinula edodes during cold storage
Hong Gao, Shuang Ye, Yani Liu, Xiuzhi Fan, Chaomin Yin, Ying Liu, Jingyu Liu, Yu Qiao, Xueling Chen, Fen Yao, Defang Shi
Food Chemistry: Molecular Sciences.2023; 6: 100172. CrossRef
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Journal of Chemical Technology & Biotechnology.2021; 96(6): 1533. CrossRef
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International Journal of Biological Macromolecules.2020; 161: 1161. CrossRef
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Lilia Sabantina, Franziska Kinzel, Thomas Hauser, Astrid Többer, Michaela Klöcker, Christoph Döpke, Robin Böttjer, Daria Wehlage, Anke Rattenholl, Andrea Ehrmann
Nanomaterials.2019; 9(3): 475. CrossRef
Algorithm for Physiological Interpretation of Transcriptome Profiling Data for Non-Model Organisms
R. F. Gubaev, V. Y. Gorshkov, L. M. Gapa, N. E. Gogoleva, E. P. Vetchinkina, Y. V. Gogolev
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Comparing the sugar profiles and primary structures of alkali-extracted water-soluble polysaccharides in cell wall between the yeast and mycelial phases from Tremella fuciformis: Hanyu Zhu , Yuan Yuan , Juan Liu , Liesheng Zheng , Liguo Chen , Aimin Ma; J. Microbiol. 2016;54(5):381-386. Published online April 20, 2016; DOI: https://doi.org/10.1007/s12275-016-5533-x

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Abstract

To gain insights into dimorphism, cell wall polysaccharides from Tremella fuciformis strains were obtained from alkaliextracted water-soluble fractions PTF-M38 (from the mycelial form), PTF-Y3 and PTF-Y8 (from the yeast form) of T. fuciformis strains were used to gain some insights into dimorphism study. Their chemical properties and structural features were investigated using gel permeation chromatography, gas chromatography, UV and IR spectrophotometry and Congo red binding reactions. The results indicated that the backbones of PTF-M38, PTF-Y3 and PTF-Y8 were configured with α-linkages with average molecular weights of 1.24, 1.08, and 1.19 kDa, respectively. PTF-M38 was mainly composed of xylose, mannose, glucose, and galactose in a ratio of 1:1.47:0.48:0.34, while PTF-Y3 and PTF-Y8 were mainly composed of xylose, mannose and glucose in a ratio of 1:1.65:4.06 and 1:1.21:0.44, respectively. The sugar profiles of PTF-M38, PTF-Y3 and PTF-Y8 were also established for further comparison. These profiles showed that all three polysaccharides contained the same sugars but in different ratios, and the carbon sources (xylose, mannose, glucose, and galactose) affected the sugar ratios within the polysaccharides.

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Reviews

REVIEW] Recent advances in the understanding of the Aspergillus fumigatus cell wall: Mark J. Lee , Donald C. Sheppard; J. Microbiol. 2016;54(3):232-242. Published online February 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6045-4

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Abstract

Over the past several decades, research on the synthesis and organization of the cell wall polysaccharides of Aspergillus fumigatus has expanded our knowledge of this important fungal structure. Besides protecting the fungus from environmental stresses and maintaining structural integrity of the organism, the cell wall is also the primary site for interaction with host tissues during infection. Cell wall polysaccharides are important ligands for the recognition of fungi by the innate immune system and they can mediate potent immunomodulatory effects. The synthesis of cell wall polysaccharides is a complicated process that requires coordinated regulation of many biosynthetic and metabolic pathways. Continuous synthesis and remodeling of the polysaccharides of the cell wall is essential for the survival of the fungus during development, reproduction, colonization and invasion. As these polysaccharides are absent from the human host, these biosynthetic pathways are attractive targets for antifungal development. In this review, we present recent advances in our understanding of Aspergillus fumigatus cell wall polysaccharides, including the emerging role of cell wall polysaccharides in the host-pathogen interaction.

Citations

Citations to this article as recorded by

Fungal β-1, 3-glucanosyltransferases: A comprehensive review on classification, catalytic mechanism and functional role
Ting-Ting Wen, Zhuo-Yu Qian, Lei Sun, Feng-Jie Cui, Xin-Yi Zan, Li-Juan Meng, Wen-Jing Sun
International Journal of Biological Macromolecules.2025; 289: 138651. CrossRef
The yeast–human coevolution: Fungal transition from passengers, colonizers, and invaders
Stefano Nenciarini, Sonia Renzi, Monica di Paola, Niccolò Meriggi, Duccio Cavalieri
WIREs Mechanisms of Disease.2024;[Epub] CrossRef
The postbiotic potential of Aspergillus oryzae – a narrative review
Yvonne Seidler, Gerald Rimbach, Kai Lüersen, Gabriel Vinderola, Ignacio R. Ipharraguerre
Frontiers in Microbiology.2024;[Epub] CrossRef
Roles of α-1,3-glucosyltransferase in growth and polysaccharides biosynthesis of Ganoderma lucidum
Haixiu Chen, Liting Zhao, Cuiping You, Jingyun Liu, Lei Chen, Zhenghua Gu, Guiyang Shi, Junxun Li, Zhongyang Ding
International Journal of Biological Macromolecules.2024; 276: 134031. CrossRef
The basic leucine zipper transcription factor MeaB is critical for biofilm formation, cell wall integrity, and virulence in Aspergillus fumigatus
Yuan Chen, Fei Gao, Xiaojin Chen, Siyuan Tao, Peiying Chen, Wei Lin, Aaron P. Mitchell
mSphere.2024;[Epub] CrossRef
Antifungal mechanism of p-anisaldehyde against Aspergillus flavus based on transcriptome analysis
Yanli Xin, Wei Zhang, Yang Lei, Shan Wei, Shuaibing Zhang, Na Li, Yuansen Hu, Yangyong Lv
LWT.2024; 195: 115844. CrossRef
Aspergillus Cell Surface Structural Analysis and Its Applications to Industrial and Medical Use
Ken Miyazawa, Takashi Umeyama, Akira Yoshimi, Keietsu Abe, Yoshitsugu Miyazaki
Medical Mycology Journal.2024; 65(3): 75. CrossRef
Structural adaptation of fungal cell wall in hypersaline environment
Liyanage D. Fernando, Yordanis Pérez-Llano, Malitha C. Dickwella Widanage, Anand Jacob, Liliana Martínez-Ávila, Andrew S. Lipton, Nina Gunde-Cimerman, Jean-Paul Latgé, Ramón Alberto Batista-García, Tuo Wang
Nature Communications.2023;[Epub] CrossRef
The Lipid Raft-Associated Protein Stomatin Is Required for Accumulation of Dectin-1 in the Phagosomal Membrane and for Full Activity of Macrophages against Aspergillus fumigatus
Marie Goldmann, Franziska Schmidt, Zoltán Cseresnyés, Thomas Orasch, Susanne Jahreis, Susann Hartung, Marc Thilo Figge, Marie von Lilienfeld-Toal, Thorsten Heinekamp, Axel A. Brakhage, Aaron P. Mitchell
mSphere.2023;[Epub] CrossRef
Antifungal effect of triclosan on Aspergillus fumigatus: quorum quenching role as a single agent and synergy with liposomal amphotericin-B
Roya Tamimi, Godfrey Kyazze, Tajalli Keshavarz
World Journal of Microbiology and Biotechnology.2022;[Epub] CrossRef
Quantitative Monitoring of Mycelial Growth of Aspergillus fumigatus in Liquid Culture by Optical Density
Ken Miyazawa, Takashi Umeyama, Yasutaka Hoshino, Keietsu Abe, Yoshitsugu Miyazaki, Slavena Vylkova, Xiaoxiao He, Takuji Oka
Microbiology Spectrum.2022;[Epub] CrossRef
Pharmacokinetic profile of sarcin and thionin from Aspergillus giganteus and in vitro validation against human fungal pathogen
Ramya Ravindhiran, Ramya Krishnamurthy, Karthiga Sivarajan, Jothi Nayaki Sekar, Kumarappan Chidambaram, Ali M Alqahtani, Kavitha Dhandapani
Bioscience Reports.2022;[Epub] CrossRef
Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species
Liyanage D. Fernando, Malitha C. Dickwella Widanage, S. Chandra Shekar, Frederic Mentink-Vigier, Ping Wang, Sungsool Wi, Tuo Wang
Journal of Structural Biology: X.2022; 6: 100070. CrossRef
Distinct Cohorts of Aspergillus fumigatus Transcription Factors Are Required for Epithelial Damage Occurring via Contact- or Soluble Effector-Mediated Mechanisms
Sayema Rahman, Norman van Rhijn, Panagiotis Papastamoulis, Darren D. Thomson, Zorana Carter, Rachael Fortune-Grant, Magnus Rattray, Michael John Bromley, Elaine Bignell
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
A 13C three-dimensional DQ-SQ-SQ correlation experiment for high-resolution analysis of complex carbohydrates using solid-state NMR
S. Chandra Shekar, Wancheng Zhao, Liyanage D. Fernando, Ivan Hung, Tuo Wang
Journal of Magnetic Resonance.2022; 336: 107148. CrossRef
MicroRNA regulation and host interaction in response to Aspergillus exposure
MANSI SHRIVASTAVA, DIKSHA PANDEY, POONAM SHARMA, RAMBIR SINGH
BIOCELL.2022; 46(2): 339. CrossRef
SfgA Renders Aspergillus flavus More Stable to the External Environment
Xiao-Yu Yuan, Jie-Ying Li, Qing-Qing Zhi, Sheng-Da Chi, Su Qu, Yan-Feng Luo, Zhu-Mei He
Journal of Fungi.2022; 8(6): 638. CrossRef
Online Biomass Monitoring Enables Characterization of the Growth Pattern of Aspergillus fumigatus in Liquid Shake Conditions
Ingo Bauer, Beate Abt, Annie Yap, Bernd Leuchtle, Hubertus Haas
Journal of Fungi.2022; 8(10): 1013. CrossRef
The putative polysaccharide synthase AfCps1 regulates Aspergillus fumigatus morphogenesis and conidia immune response in mouse bone marrow-derived macrophages
Sha Wang, Anjie Yuan, Liping Zeng, Sikai Hou, Meng Wang, Lei Li, Zhendong Cai, Guowei Zhong
Journal of Microbiology.2021; 59(1): 64. CrossRef
Unraveling the sugar code: the role of microbial extracellular glycans in plant–microbe interactions
Alan Wanke, Milena Malisic, Stephan Wawra, Alga Zuccaro, Andreas Weber
Journal of Experimental Botany.2021; 72(1): 15. CrossRef
Aspergillus fumigatus In-Host HOG Pathway Mutation for Cystic Fibrosis Lung Microenvironment Persistence
Brandon S. Ross, Lotus A. Lofgren, Alix Ashare, Jason E. Stajich, Robert A. Cramer, J. Andrew Alspaugh
mBio.2021;[Epub] CrossRef
Fungal α-1,3-Glucan as a New Pathogen-Associated Molecular Pattern in the Insect Model Host Galleria mellonella
Sylwia Stączek, Agnieszka Zdybicka-Barabas, Iwona Wojda, Adrian Wiater, Paweł Mak, Piotr Suder, Krzysztof Skrzypiec, Małgorzata Cytryńska
Molecules.2021; 26(16): 5097. CrossRef
Genetic and structural validation of phosphomannomutase as a cell wall target in Aspergillus fumigatus
Yuanwei Zhang, Wenxia Fang, Olawale G. Raimi, Deborah E. A. Lockhart, Andrew T. Ferenbach, Ling Lu, Daan M. F. van Aalten
Molecular Microbiology.2021; 116(1): 245. CrossRef
The Influence of Anomeric Configuration and Aglycone Structure on the Outcome of Acid‐Promoted Ring Contraction in 2,3‐Di‐O‐Silylated S‐Galactopyranosides
Polina I. Abronina, Alexander I. Zinin, Nelly N. Malysheva, Maxim Y. Karpenko, Natalya G. Kolotyrkina, Leonid O. Kononov
ChemistrySelect.2021; 6(24): 6223. CrossRef
Effect of Carbohydrate Substrate from Starch-Containing Raw Materials on the Composition of Glucans in the Micromycete Aspergillus niger VKPM F-171 Biomass
N.Yu. Sharova, B.S. Manzhieva, A.A. Printseva, T.V. Vybornova, A.S. Miroshnik, I.V. Kruchina-Bogdanov, I.A. Orekhova
Biotekhnologiya.2020; 36(5): 41. CrossRef
Cell wall polysaccharides from pathogenic fungi for diagnosis of fungal infectious disease
Dandy Ahamefula Osibe, Shuhan Lei, Bin Wang, Cheng Jin, Wenxia Fang
Mycoses.2020; 63(7): 644. CrossRef
What Are the Functions of Chitin Deacetylases in Aspergillus fumigatus?
Isabelle Mouyna, Sarah Dellière, Anne Beauvais, Fabrice Gravelat, Brendan Snarr, Mélanie Lehoux, Caitlin Zacharias, Yan Sun, Steven de Jesus Carrion, Eric Pearlman, Donald C. Sheppard, Jean-Paul Latgé
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef
The Fungal Cell Wall: Candida, Cryptococcus, and Aspergillus Species
Rocio Garcia-Rubio, Haroldo C. de Oliveira, Johanna Rivera, Nuria Trevijano-Contador
Frontiers in Microbiology.2020;[Epub] CrossRef
Usefulness of serum galactomannan in initiating and modifying antifungal therapy in children with cancer and persistent high‐risk febrile neutropenia
Matías Delgado‐Araneda, Romina Valenzuela, Verónica de la Maza, Marcela Rabello, Ana M. Álvarez, Verónica Contardo, Marcela Zubieta, Valentina Gutierrez, Ximena Claverie, Juan P. Torres, Carmen Salgado, Juan Tordecilla, Mónica Varas, Carmen L. Avilés, Mar
Mycoses.2020; 63(8): 802. CrossRef
The mechanisms of hyphal pellet formation mediated by polysaccharides, α-1,3-glucan and galactosaminogalactan, in Aspergillus species
Ken Miyazawa, Akira Yoshimi, Keietsu Abe
Fungal Biology and Biotechnology.2020;[Epub] CrossRef
Dimethylformamide Inhibits Fungal Growth and Aflatoxin B1 Biosynthesis in Aspergillus flavus by Down-Regulating Glucose Metabolism and Amino Acid Biosynthesis
Lin Pan, Peng Chang, Jing Jin, Qingli Yang, Fuguo Xing
Toxins.2020; 12(11): 683. CrossRef
Overview of the Interplay Between Cell Wall Integrity Signaling Pathways and Membrane Lipid Biosynthesis in Fungi: Perspectives forAspergillus fumigatus
João Henrique T.M. Fabri, Marina C. Rocha, Iran Malavazi
Current Protein & Peptide Science.2020; 21(3): 265. CrossRef
The Myosin Motor Domain-Containing Chitin Synthases Are Involved in Cell Wall Integrity and Sensitivity to Antifungal Proteins in Penicillium digitatum
Mónica Gandía, Sandra Garrigues, Begoña Bolós, Paloma Manzanares, Jose F. Marcos
Frontiers in Microbiology.2019;[Epub] CrossRef
Cell surface display of proteins on filamentous fungi
Jesús Urbar-Ulloa, Paul Montaño-Silva, Ana Sofía Ramírez-Pelayo, Elisa Fernández-Castillo, Lorena Amaya-Delgado, Benjamín Rodríguez-Garay, Jorge Verdín
Applied Microbiology and Biotechnology.2019; 103(17): 6949. CrossRef
Transcriptomic Insights into Benzenamine Effects on the Development, Aflatoxin Biosynthesis, and Virulence of Aspergillus flavus
Mingguan Yang, Laifeng Lu, Shuhua Li, Jing Zhang, Zhenjing Li, Shufen Wu, Qingbin Guo, Huanhuan Liu, Changlu Wang
Toxins.2019; 11(2): 70. CrossRef
Both Galactosaminogalactan and α-1,3-Glucan Contribute to Aggregation of Aspergillus oryzae Hyphae in Liquid Culture
Ken Miyazawa, Akira Yoshimi, Motoaki Sano, Fuka Tabata, Asumi Sugahara, Shin Kasahara, Ami Koizumi, Shigekazu Yano, Tasuku Nakajima, Keietsu Abe
Frontiers in Microbiology.2019;[Epub] CrossRef
Aspergillus fumigatus Mnn9 is responsible for mannan synthesis and required for covalent linkage of mannoprotein to the cell wall
Ting Du, Haomiao Ouyang, Josef Voglmeir, Iain B.H. Wilson, Cheng Jin
Fungal Genetics and Biology.2019; 128: 20. CrossRef
Aspergillus fumigatus phosphoethanolamine transferase gene gpi7 is required for proper transportation of the cell wall GPI-anchored proteins and polarized growth
Haomiao Ouyang, Ting Du, Hui Zhou, Iain B. H. Wilson, Jinghua Yang, Jean-Paul Latgé, Cheng Jin
Scientific Reports.2019;[Epub] CrossRef
Klebsiella pneumoniae prevents spore germination and hyphal development of Aspergillus species
M. F. Nogueira, L. Pereira, S. Jenull, K. Kuchler, T. Lion
Scientific Reports.2019;[Epub] CrossRef
Overexpression of Aspergillus nidulans α-1,3-glucan synthase increases cellular adhesion and causes cell wall defects
Xiaoxiao He, Shengnan Li, Susan G W Kaminskyj
Medical Mycology.2018; 56(5): 645. CrossRef
The Aspergillus fumigatus Sialidase (Kdnase) Contributes to Cell Wall Integrity and Virulence in Amphotericin B-Treated Mice
Jason R. Nesbitt, Elizabeth Y. Steves, Cole R. Schonhofer, Alissa Cait, Sukhbir S. Manku, Juliana H. F. Yeung, Andrew J. Bennet, Kelly M. McNagny, Jonathan C. Choy, Michael R. Hughes, Margo M. Moore
Frontiers in Microbiology.2018;[Epub] CrossRef
Protein Kinase A and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus
Leandro José de Assis, Adriana Manfiolli, Eliciane Mattos, João H. T. Marilhano Fabri, Iran Malavazi, Ilse D. Jacobsen, Matthias Brock, Robert A. Cramer, Arsa Thammahong, Daisuke Hagiwara, Laure Nicolas Annick Ries, Gustavo Henrique Goldman, Reinhard Fisc
mBio.2018;[Epub] CrossRef
A Ring Contraction of 2,3-Di-O-Silylated Thiopyranosides To Give Thiofuranosides under Mildly Acidic Conditions
Polina I. Abronina, Nelly N. Malysheva, Veronika V. Litvinenko, Alexander I. Zinin, Natalya G. Kolotyrkina, Leonid O. Kononov
Organic Letters.2018; 20(19): 6051. CrossRef
Aspergillus-specific antibodies – Targets and applications
Max Schubert, Holger Spiegel, Stefan Schillberg, Greta Nölke
Biotechnology Advances.2018; 36(4): 1167. CrossRef
Molecular Mass and Localization of α-1,3-Glucan in Cell Wall Control the Degree of Hyphal Aggregation in Liquid Culture of Aspergillus nidulans
Ken Miyazawa, Akira Yoshimi, Shin Kasahara, Asumi Sugahara, Ami Koizumi, Shigekazu Yano, Satoshi Kimura, Tadahisa Iwata, Motoaki Sano, Keietsu Abe
Frontiers in Microbiology.2018;[Epub] CrossRef
Aspergillusin chronic lung disease: Modeling what goes on in the airways
Takahiro Takazono, Donald C. Sheppard
Medical Mycology.2017; 55(1): 39. CrossRef
Sporothrix schenckii sensu stricto and Sporothrix brasiliensis Are Differentially Recognized by Human Peripheral Blood Mononuclear Cells
José A. Martínez-Álvarez, Luis A. Pérez-García, Erika Mellado-Mojica, Mercedes G. López, Iván Martínez-Duncker, Leila M. Lópes-Bezerra, Héctor M. Mora-Montes
Frontiers in Microbiology.2017;[Epub] CrossRef
Immune responses to invasive aspergillosis: new understanding and therapeutic opportunities
Tobias M. Hohl
Current Opinion in Infectious Diseases.2017; 30(4): 364. CrossRef
Host Soluble Mediators: Defying the Immunological Inertness of Aspergillus fumigatus Conidia
Sarah Sze Wah Wong, Vishukumar Aimanianda
Journal of Fungi.2017; 4(1): 3. CrossRef
Immune Recognition of Fungal Polysaccharides
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Journal of Fungi.2017; 3(3): 47. CrossRef
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Ivey Geoghegan, Gero Steinberg, Sarah Gurr
Trends in Microbiology.2017; 25(12): 957. CrossRef
Immunity against fungi
Michail S. Lionakis, Iliyan D. Iliev, Tobias M. Hohl
JCI Insight.2017;[Epub] CrossRef
Human fungal pathogens: Why should we learn?
Jeong-Yoon Kim
Journal of Microbiology.2016; 54(3): 145. CrossRef
Proteomic analysis of Aspergillus fumigatus – clinical implications
Nicola M. Moloney, Rebecca A. Owens, Sean Doyle
Expert Review of Proteomics.2016; 13(7): 635. CrossRef
How to invade a susceptible host: cellular aspects of aspergillosis
Sven Krappmann
Current Opinion in Microbiology.2016; 34: 136. CrossRef
The Use of Pyranoside-into-Furanoside Rearrangement and Controlled O(5) → O(6) Benzoyl Migration as the Basis of a Synthetic Strategy To Assemble (1→5)- and (1→6)-Linked Galactofuranosyl Chains
Dmitry A. Argunov, Vadim B. Krylov, Nikolay E. Nifantiev
Organic Letters.2016; 18(21): 5504. CrossRef

REVIEW] The contribution of Aspergillus fumigatus stress responses to virulence and antifungal resistance: Neil A. Brown , Gustavo H. Goldman; J. Microbiol. 2016;54(3):243-253. Published online February 27, 2016; DOI: https://doi.org/10.1007/s12275-016-5510-4

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Abstract

Invasive aspergillosis has emerged as one of the most common life-threatening fungal disease of humans. The emergence of antifungal resistant pathogens represents a current and increasing threat to society. In turn, new strategies to combat fungal infection are urgently required. Fungal adaptations to stresses experienced within the human host are a prerequisite for the survival and virulence strategies of the pathogen. Here, we review the latest information on the signalling pathways in Aspergillus fumigatus that contribute to stress adaptations and virulence, while highlighting their potential as targets for the development of novel combinational antifungal therapies.

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Josephine L. Battersby, David A. Stevens, Robert H. A. Coutts, Vladimír Havlíček, Joe L. Hsu, Gabriele Sass, Ioly Kotta-Loizou
Journal of Fungi.2024; 10(8): 585. CrossRef
Synergistic effects of putative Ca2+-binding sites of calmodulin in fungal development, temperature stress and virulence ofAspergillus fumigatus
Xingyue Li, Ruoyun Feng, Pan Luo, Yuanwei Zhang, Ling Lu
Virulence.2024;[Epub] CrossRef
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Prasad Gandham, Kanniah Rajasekaran, Christine Sickler, Harikrishnan Mohan, Matthew Gilbert, Niranjan Baisakh
Stress Biology.2024;[Epub] CrossRef
Pleiotropic functions of SscA on the asexual spore of the human pathogenic fungus Aspergillus fumigatus
Ye-Eun Son, Jiwoo Han, Kyung-Tae Lee, Hee-Soo Park
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Tamás Emri, Károly Antal, Kinga Varga, Barnabás Csaba Gila, István Pócsi
Journal of Fungi.2024; 10(3): 221. CrossRef
Response of Fusarium oxysporum soil isolate to amphotericin B and fluconazole at the proteomic level
I. V. da S. Amatto, F. A. de O. Simões, N. G. da R. Garzon, C. L. Marciano, R. R. da Silva, H. Cabral
Brazilian Journal of Microbiology.2024; 55(3): 2557. CrossRef
tRNA hypomodification facilitates 5-fluorocytosine resistance via cross-pathway control system activation in Aspergillus fumigatus
Alexander Bruch, Valentina Lazarova, Maximilian Berg, Thomas Krüger, Sascha Schäuble, Abdulrahman A Kelani, Birte Mertens, Pamela Lehenberger, Olaf Kniemeyer, Stefanie Kaiser, Gianni Panagiotou, Fabio Gsaller, Matthew G Blango
Nucleic Acids Research.2024;[Epub] CrossRef
A host defense peptide mimetic, brilacidin, potentiates caspofungin antifungal activity against human pathogenic fungi
Thaila Fernanda dos Reis, Patrícia Alves de Castro, Rafael Wesley Bastos, Camila Figueiredo Pinzan, Pedro F. N. Souza, Suzanne Ackloo, Mohammad Anwar Hossain, David Harold Drewry, Sondus Alkhazraji, Ashraf S. Ibrahim, Hyunil Jo, Jorge D. Lightfoot, Emily
Nature Communications.2023;[Epub] CrossRef
Pathogenicity and virulence of Aspergillus fumigatus
Kayleigh Earle, Clara Valero, Daniel P. Conn, George Vere, Peter C. Cook, Michael J. Bromley, Paul Bowyer, Sara Gago
Virulence.2023;[Epub] CrossRef
Putative Core Transcription Factors Affecting Virulence in Aspergillus flavus during Infection of Maize
Matthew K. Gilbert, Brian M. Mack, Matthew D. Lebar, Perng-Kuang Chang, Stephanie R. Gross, Rebecca R. Sweany, Jeffrey W. Cary, Kanniah Rajasekaran
Journal of Fungi.2023; 9(1): 118. CrossRef
Fungi’s Swiss Army Knife: Pleiotropic Effect of Melanin in Fungal Pathogenesis during Cattle Mycosis
Víctor Romero, Carolina Kalinhoff, Luis Rodrigo Saa, Aminael Sánchez
Journal of Fungi.2023; 9(9): 929. CrossRef
Afu-Emi1 Contributes to Stress Adaptation and Voriconazole Susceptibility in Aspergillus fumigatus
Jufang Tan, Heng Zhang, Yi Sun, Lujuan Gao, Cezar M. Khursigara
Microbiology Spectrum.2023;[Epub] CrossRef
COVID-19-Associated Pulmonary Aspergillosis Isolates Are Genomically Diverse but Similar to Each Other in Their Responses to Infection-Relevant Stresses
Matthew E. Mead, Patrícia Alves de Castro, Jacob L. Steenwyk, Jean-Pierre Gangneux, Martin Hoenigl, Juergen Prattes, Riina Rautemaa-Richardson, Hélène Guegan, Caroline B. Moore, Cornelia Lass-Flörl, Florian Reizine, Clara Valero, Norman Van Rhijn, Michael
Microbiology Spectrum.2023;[Epub] CrossRef
Host-derived reactive oxygen species trigger activation of the Candida albicans transcription regulator Rtg1/3
Mazen Oneissi, Melissa R. Cruz, Bernardo Ramírez-Zavala, Elena Lindemann-Perez, Joachim Morschhäuser, Danielle A. Garsin, J. Christian Perez, Aaron P. Mitchell
PLOS Pathogens.2023; 19(9): e1011692. CrossRef
The high osmolarity glycerol (HOG) pathway in fungi†
Hajar Yaakoub, Norma Silvia Sanchez, Laura Ongay-Larios, Vincent Courdavault, Alphonse Calenda, Jean-Philippe Bouchara, Roberto Coria, Nicolas Papon
Critical Reviews in Microbiology.2022; 48(6): 657. CrossRef
Stress Responses Elicited by Glucose Withdrawal in Aspergillus fumigatus
Tamás Emri, Károly Antal, Barnabás Gila, Andrea P. Jónás, István Pócsi
Journal of Fungi.2022; 8(11): 1226. CrossRef
PHYTONICIDIC PROPERTIES OF PLANTS REGARDING FUNGI OF THE ASPERGILLUS GENUS
A. Karasenko, S. Peredera
Sučasne ptahìvnictvo.2022;[Epub] CrossRef
Strategies Shaping the Transcription of Carbohydrate-Active Enzyme Genes in Aspergillus nidulans
Barnabás Cs. Gila, Károly Antal, Zsuzsanna Birkó, Judit Sz. Keserű, István Pócsi, Tamás Emri
Journal of Fungi.2022; 8(1): 79. CrossRef
An evolutionary genomic approach reveals both conserved and species-specific genetic elements related to human disease in closely related Aspergillus fungi
Matthew E Mead, Jacob L Steenwyk, Lilian P Silva, Patrícia A de Castro, Nauman Saeed, Falk Hillmann, Gustavo H Goldman, Antonis Rokas, A Mitchell
Genetics.2021;[Epub] CrossRef
Flavonoids Modulate the Accumulation of Toxins From Aspergillus flavus in Maize Kernels
Lina Castano-Duque, Matthew K. Gilbert, Brian M. Mack, Matthew D. Lebar, Carol H. Carter-Wientjes, Christine M. Sickler, Jeffrey W. Cary, Kanniah Rajasekaran
Frontiers in Plant Science.2021;[Epub] CrossRef
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Patrícia Alves de Castro, Clara Valero, Jéssica Chiaratto, Ana Cristina Colabardini, Lakhansing Pardeshi, Lilian Pereira Silva, Fausto Almeida, Marina Campos Rocha, Roberto Nascimento Silva, Iran Malavazi, Wenyue Du, Paul S. Dyer, Matthias Brock, Flávio V
mBio.2021;[Epub] CrossRef
Fungal G-Protein-Coupled Receptors: A Promising Mediator of the Impact of Extracellular Signals on Biosynthesis of Ochratoxin A
Jing Gao, Xinge Xu, Kunlun Huang, Zhihong Liang
Frontiers in Microbiology.2021;[Epub] CrossRef
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Jasmin Binder, Yana Shadkchan, Nir Osherov, Sven Krappmann
Frontiers in Microbiology.2020;[Epub] CrossRef
Putative Membrane Receptors Contribute to Activation and Efficient Signaling of Mitogen-Activated Protein Kinase Cascades during Adaptation of Aspergillus fumigatus to Different Stressors and Carbon Sources
Lilian Pereira Silva, Dean Frawley, Leandro José de Assis, Ciara Tierney, Alastair B. Fleming, Ozgur Bayram, Gustavo Henrique Goldman, Aaron P. Mitchell
mSphere.2020;[Epub] CrossRef
Evolving moldy murderers: Aspergillus section Fumigati as a model for studying the repeated evolution of fungal pathogenicity
Antonis Rokas, Matthew E. Mead, Jacob L. Steenwyk, Nicholas H. Oberlies, Gustavo H. Goldman, Donald C. Sheppard
PLOS Pathogens.2020; 16(2): e1008315. CrossRef
Aspergillus fumigatus G-Protein Coupled Receptors GprM and GprJ Are Important for the Regulation of the Cell Wall Integrity Pathway, Secondary Metabolite Production, and Virulence
Aílton Pereira da Costa Filho, Guilherme Thomaz Pereira Brancini, Patrícia Alves de Castro, Clara Valero, Jaire Alves Ferreira Filho, Lilian Pereira Silva, Marina Campos Rocha, Iran Malavazi, João Guilherme de Moraes Pontes, Taícia Fill, Roberto Nasciment
mBio.2020;[Epub] CrossRef
Phenotypic plasticity and the evolution of azole resistance in Aspergillus fumigatus; an expression profile of clinical isolates upon exposure to itraconazole
Margriet W. J. Hokken, Jan Zoll, Jordy P. M. Coolen, Bas J. Zwaan, Paul E. Verweij, Willem J. G. Melchers
BMC Genomics.2019;[Epub] CrossRef
Aspergillus fumigatus calcium-responsive transcription factors regulate cell wall architecture promoting stress tolerance, virulence and caspofungin resistance
Patrícia Alves de Castro, Ana Cristina Colabardini, Adriana Oliveira Manfiolli, Jéssica Chiaratto, Lilian Pereira Silva, Eliciane Cevolani Mattos, Giuseppe Palmisano, Fausto Almeida, Gabriela Felix Persinoti, Laure Nicolas Annick Ries, Laura Mellado, Mari
PLOS Genetics.2019; 15(12): e1008551. CrossRef
How Environmental Fungi Cause a Range of Clinical Outcomes in Susceptible Hosts
Steven T. Denham, Morgan A. Wambaugh, Jessica C.S. Brown
Journal of Molecular Biology.2019; 431(16): 2982. CrossRef
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The crystal structure of the D-alanine-D-alanine ligase from Acinetobacter baumannii suggests a flexible conformational change in the central domain before nucleotide binding: Kim-Hung Huynh , Myoung-ki Hong , Clarice Lee , Huyen-Thi Tran , Sang Hee Lee , Yeh-Jin Ahn , Sun-Shin Cha , Lin-Woo Kang; J. Microbiol. 2015;53(11):776-782. Published online October 28, 2015; DOI: https://doi.org/10.1007/s12275-015-5475-8

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Abstract

Acinetobacter baumannii, which is emerging as a multidrugresistant nosocomial pathogen, causes a number of diseases, including pneumonia, bacteremia, meningitis, and skin infections. With ATP hydrolysis, the D-alanine-D-alanine ligase (DDL) catalyzes the synthesis of D-alanyl-D-alanine, which is an essential component of bacterial peptidoglycan. In this study, we determined the crystal structure of DDL from A. baumannii (AbDDL) at a resolution of 2.2 Å. The asymmetric unit contained six protomers of AbDDL. Five protomers had a closed conformation in the central domain, while one protomer had an open conformation in the central domain. The central domain with an open conformation did not interact with crystallographic symmetry-related protomers and the conformational change of the central domain was not due to crystal packing. The central domain of AbDDL can have an ensemble of the open and closed conformations before the binding of substrate ATP. The conformational change of the central domain is important for the catalytic activity and the detail information will be useful for the development of inhibitors against AbDDL and putative antibacterial agents against A. baumannii. The AbDDL structure was compared with that of other DDLs that were in complex with potent inhibitors and the catalytic activity of AbDDL was confirmed using enzyme kinetics assays.

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In Silico Design and In Vitro Assessment of Bicyclic Trifluoromethylated Pyrroles as New Antibacterial and Antifungal Agents
Diana Hodyna, Anton Klipkov, Maryna Kachaeva, Yurii Shulha, Igor Gerus, Larysa Metelytsia, Vasyl Kovalishyn
Chemistry & Biodiversity.2024;[Epub] CrossRef
Genome-Scale Metabolic Modeling Reveals Metabolic Alterations of Multidrug-Resistant Acinetobacter baumannii in a Murine Bloodstream Infection Model
Jinxin Zhao, Yan Zhu, Jiru Han, Yu-Wei Lin, Michael Aichem, Jiping Wang, Ke Chen, Tony Velkov, Falk Schreiber, Jian Li
Microorganisms.2020; 8(11): 1793. CrossRef
Identification of natural inhibitors against Acinetobacter baumannii d-alanine-d-alanine ligase enzyme: A multi-spectrum in silico approach
Sajjad Ahmad, Saad Raza, Sumra Wajid Abbasi, Syed Sikander Azam
Journal of Molecular Liquids.2018; 262: 460. CrossRef
Molecular characterization of SCO0765 as a cellotriose releasing endo-β-1,4-cellulase from Streptomyces coelicolor A(3)
Joo-Bin Hong, Vijayalakshmi Dhakshnamoorthy, Chang-Ro Lee
Journal of Microbiology.2016; 54(9): 626. CrossRef

The Identification of Six Novel Proteins with Fibronectin or Collagen Type І Binding Activity from Streptococcus suis Serotype 2: Hui Zhang , Junxi Zheng , Li Yi , Yue Li , Zhe Ma , Hongjie Fan , Chengping Lu; J. Microbiol. 2014;52(11):963-969. Published online October 31, 2014; DOI: https://doi.org/10.1007/s12275-014-4311-x

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Abstract

Streptococcus suis, a major swine pathogen, is an emerging zoonotic agent that causes meningitis and septic shock. Bacterial cell wall and secreted proteins are often involved in interactions with extracellular matrix proteins (ECMs), which play important roles in the initial steps of pathogenesis. In this study, 2D SDS-PAGE, western blotting-based binding affinity measurements, and microtiter plate binding assays were used to identify cell wall and secreted proteins from S. suis that interact with fibronectin and collagen type І. We identified six proteins from S. suis, including three proteins (translation elongation factor G, oligopeptide-binding protein OppA precursor, and phosphoglycerate mutase) that show both fibronectin and collagen type І binding activity. To the best of our knowledge, these three newly identified proteins had no previously reported fibronectin or collagen type І binding activity. Overall, the aim in this study was to identify proteins with ECM binding activity from S. suis and it represents the first report of six new proteins from S. suis that interact with fibronectin or collagen type І.

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Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig
Yanyan Zhang, Rui Li, Qian Li, Yongwei Zhu, Xiaopei Yang, Di Zhao, Bingbing Zong
BMC Veterinary Research.2023;[Epub] CrossRef
Schistosoma mansoni phosphoglycerate mutase: a glycolytic ectoenzyme with thrombolytic potential
David B. Pirovich, Akram A. Da’dara, Patrick J. Skelly
Parasite.2022; 29: 41. CrossRef
Quantitative proteomic analysis reveals that serine/threonine kinase is involved in Streptococcus suis virulence and adaption to stress conditions
Haodan Zhu, Junming Zhou, Dandan Wang, Zhengyu Yu, Bin Li, Yanxiu Ni, Kongwang He
Archives of Microbiology.2021; 203(7): 4715. CrossRef
Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2
Quan Li, Yang Fu, Genglin Guo, Zhuohao Wang, Wei Zhang
AMB Express.2020;[Epub] CrossRef
Identification of two adhesins of Actinobacillus seminis
J. Fernando Montes-García, Delil A. Chincoya Martinez, Sergio Vaca Pacheco, Candelario Vázquez Cruz, Patricia Sanchez Alonso, Juan Xicohtencatl Cortes, Hector Trujillo-Ruiz, Erasmo Negrete-Abascal
Small Ruminant Research.2018; 167: 100. CrossRef
Characterization and functional analysis of PnuC that is involved in the oxidative stress tolerance and virulence of Streptococcus suis serotype 2
Quan Li, Yuhang Zhang, Du Dechao, Yu Yanfei, Wei Zhang
Veterinary Microbiology.2018; 216: 198. CrossRef
Understanding the virulence of Streptococcus suis : A veterinary, medical, and economic challenge
B. Haas, D. Grenier
Médecine et Maladies Infectieuses.2018; 48(3): 159. CrossRef
Factor H specifically capture novel Factor H-binding proteins of Streptococcus suis and contribute to the virulence of the bacteria
Quan Li, Caifeng Ma, Yang Fu, Yanan He, Yanfei Yu, Dechao Du, Huochun Yao, Chengping Lu, Wei Zhang
Microbiological Research.2017; 196: 17. CrossRef
The surface-displayed chaperones GroEL and DnaK of Mycoplasma pneumoniae interact with human plasminogen and components of the extracellular matrix
Lisa Hagemann, Anne Gründel, Enno Jacobs, Roger Dumke
Pathogens and Disease.2017;[Epub] CrossRef
Initial steps of the pathogenesis of the infection caused byStreptococcus suis: fighting against nonspecific defenses
Mariela Segura, Cynthia Calzas, Daniel Grenier, Marcelo Gottschalk
FEBS Letters.2016; 590(21): 3772. CrossRef
Interactions of surface-displayed glycolytic enzymes of Mycoplasma pneumoniae with components of the human extracellular matrix
Anne Gründel, Enno Jacobs, Roger Dumke
International Journal of Medical Microbiology.2016; 306(8): 675. CrossRef
Identification of Novel Laminin- and Fibronectin-binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2
Quan Li, Hanze Liu, Dechao Du, Yanfei Yu, Caifeng Ma, Fangfang Jiao, Huochun Yao, Chengping Lu, Wei Zhang
Frontiers in Cellular and Infection Microbiology.2015;[Epub] CrossRef

Identification and Characterization of a Class III Chitin Synthase Gene of Moniliophthora perniciosa, the Fungus That Causes Witches' Broom Disease of Cacao: Catiane S. Souza , Bruno M. Oliveira , Gustavo G. L. Costa , Albert Schriefer , Alessandra Selbach-Schnadelbach , Ana Paula T. Uetanabaro , Carlos P. Pirovani , Goncalo A. G. Pereira , Alex G. Taranto , Julio Cezar de M. Cascardo , Aristoteles Goes-Neto; J. Microbiol. 2009;47(4):431-440. Published online September 9, 2009; DOI: https://doi.org/10.1007/s12275-008-0166-3

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Abstract: Chitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin, one of the main components of fungal cell wall. Class III chitin synthases act directly in the formation of the cell wall. They catalyze the conversion of the immediate precursor of chitin and are responsible for the majority of chitin synthesis in fungi. As such, they are highly specific molecular targets for drugs that can inhibit the growth and development of fungal pathogens. In this work, we have identified and characterized a chitin synthase gene of Moniliophthora perniciosa (Mopchs) by primer walking. The complete gene sequence is 3,443 bp, interrupted by 13 small introns, and comprises a cDNA with an ORF with 2,739 bp, whose terminal region was experimentally determined, encoding a protein with 913 aa that harbors all the motifs and domains typically found in class III chitin synthases. This is the first report on the characterization of a chitin synthase gene, its mature transcription product, and its putative protein in basidioma and secondary mycelium stages of M. perniciosa, a basidiomycotan fungus that causes witches’ broom disease of cacao.

Enhanced Secretion of Cell Wall Bound Enolase into Culture Medium by the soo1-1 Mutation of Saccharomyces cerevisiae: Ki-Hyun Kim , Hee-Moon Park; J. Microbiol. 2004;42(3):248-252.; DOI: https://doi.org/2080 [pii]

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Abstract: In order to identify the protein(s) secreted into culture medium by the soo1-1/ret1-1 mutation of Saccharomyces cerevisiae, proteins from the culture medium of cells grown at permissive (28^oC) and non-permissive temperatures (37^oC), were analyzed. Comparison of protein bands separated by SDS-PAGE identified a prominent band of 47-kDa band from a mutant grown at 37^oC. N-terminal amino acid sequencing of this 47-kDa protein showed high identity with enolases 1 and 2. Western blot analysis revealed that most of the cell wall-bound enolase was released into the culture medium of the mutant grown at 37^oC, some of which were separated as those with lower molecular weights. Our results, presented here, indicate the impairment of cell wall enolase biogenesis and assembly by the soo1-1/ret1-1 mutation of S. cerevisiae.

Heavy Metal Biosorption and its Significance to Metal Tolerance of Streptomycetes: Jae-young Rho , Jae-heon Kim; J. Microbiol. 2002;40(1):51-54.

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Abstract: Heavy metal adsorptions of four streptomycetes were compared with each other. Among the test strains, Streptomyces viridochromogenes showed the most efficient metal binding activity, which was carried out by cell wall as well as freeze-dried mycelium. An order of adsorption potential (zinc > copper > lead > cadmium) was observed in single metal reactions, whereas this adsorption order was disturbed in mixed-metal reactions. The metal adsorption reactions were very fast, pH dependent and culture age-independent, suggestive of a physico-chemical reaction between cell wall components and heavy metal ions. The metal tolerant stains presented the weakest adsorbing activity, indicating that the metal biosorption was not the basis of the metal tolerance.

Characterization of Cell Wall Proteins from the soo1-1/ret1-1 Mutant of Saccharomyces cerevisiae: Dong-Won Lee , Ki-Hyun Kim , Se-Chul Chun , Hee-Moon Park; J. Microbiol. 2002;40(3):219-223.

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Abstract: In order to investigate the function of Soo1p/[alpha]-COP during post-translational modification and intracellular transport of cell wall proteins in Saccharomyces cerevisiae, cell wall proteins from the soo1-1/ret1-1 mutant cells were analyzed. SDS-PAGE analysis of biotin labeled cell wall proteins suggested that the soo1-1 mutation impairs post-translational modification of cell wall proteins, such as N- and/or O-glycosylation. Analysis of cell wall proteins with antibodies against [beta]-1,3-glucan and [beta]-1,6-glucan revealed alteration of the linkage between cell wall proteins and [beta]-glucans in the soo1-1 mutant cells. Compositional sugar analysis of the cell wall proteins also suggested that the soo1-1 mutation impairs glycosylation of cell wall protein in the ER, which is crucial for the maintenance of cell wall integrity.

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