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- Characterization of staphylococcal endolysin LysSAP33 possessing untypical domain composition
- Jun-Hyeok Yu , Do-Won Park , Jeong-A Lim , Jong-Hyun Park
- J. Microbiol. 2021;59(9):840-847. Published online August 12, 2021
- DOI: https://doi.org/10.1007/s12275-021-1242-1
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- 5 Web of Science
- 5 Crossref
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Abstract
- Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent. Many endolysins on staphylococcal phages have been identified and applied extensively against Staphylococcus spp. Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins. However, relatively little interest has been paid to LysKunlike endolysin and a few of them has been characterized. An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD). A green fluorescence assay using a fusion protein for Lys- SAP33_CBD indicated that the CBD domain (157-251 aa) was bound to the peptidoglycan of S. aureus. The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy. Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations. In addition, it showed a higher activity against biofilms than LysK-like endolysin. This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.
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Citations
Citations to this article as recorded by
- Phage-Derived Endolysins Against Resistant Staphylococcus spp.: A Review of Features, Antibacterial Activities, and Recent Applications
Mina Golban, Javad Charostad, Hossein Kazemian, Hamid Heidari
Infectious Diseases and Therapy.2024;[Epub] CrossRef - Molecular Machinery of the Triad Holin, Endolysin, and Spanin: Key
Players Orchestrating Bacteriophage-Induced Cell Lysis and their
Therapeutic Applications
Safia Samir
Protein & Peptide Letters.2024; 31(2): 85. CrossRef - A Novel Truncated CHAP Modular Endolysin, CHAPSAP26-161, That Lyses Staphylococcus aureus, Acinetobacter baumannii, and Clostridioides difficile, and Exhibits Therapeutic Effects in a Mouse Model of A. baumannii Infection
Yoon-Jung Choi, Shukho Kim, Ram Hari Dahal, Jungmin Kim
Journal of Microbiology and Biotechnology.2024; 34(8): 1718. CrossRef - Therapeutic potential of bacteriophage endolysins for infections caused by Gram-positive bacteria
He Liu, Zhen Hu, Mengyang Li, Yi Yang, Shuguang Lu, Xiancai Rao
Journal of Biomedical Science.2023;[Epub] CrossRef - Endolysin, a Promising Solution against Antimicrobial Resistance
Mujeeb ur Rahman, Weixiao Wang, Qingqing Sun, Junaid Ali Shah, Chao Li, Yanmei Sun, Yuanrui Li, Bailing Zhang, Wei Chen, Shiwei Wang
Antibiotics.2021; 10(11): 1277. CrossRef
- Phage-Derived Endolysins Against Resistant Staphylococcus spp.: A Review of Features, Antibacterial Activities, and Recent Applications
Research Support, Non-U.S. Gov't
- Fatty Acid Biosynthesis in Eukaryotic Photosynthetic Microalgae: Identification of a Microsomal Delta 12 Desaturase in Chlamydomonas reinhardtii
- Xiaoyuan Chi , Xiaowen Zhang , Xiangyu Guan , Ling Ding , Youxun Li , Mingqing Wang , Hanzhi Lin , Song Qin
- J. Microbiol. 2008;46(2):189-201. Published online June 11, 2008
- DOI: https://doi.org/10.1007/s12275-007-0223-3
- 35 View
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- 39 Scopus
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Abstract
- Polyunsaturated fatty acids (PUFAs) are important components of infant and adult nutrition because they serve as structural elements of cell membranes. Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents. In this study, the genome-wide characterization of the fatty acid desaturases from seven eukaryotic photosynthetic microalgae was undertaken according to the conserved histidine-rich motifs and phylogenetic profiles. Analysis of these genomes provided insight into the origin and evolution of the pathway of fatty acid biosynthesis in eukaryotic plants. In addition, the candidate enzyme from Chlamydomonas reinhardtii with the highest similarity to the microsomal Δ12 desaturase of Chlorella vulgaris was isolated, and its function was verified by heterologous expression in yeast (Saccharomyces cerevisiae).
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