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- Pioneering strategies for overcoming bacterial drug resistance (2026)
- Advancing microbial engineering through synthetic biology (2025)
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- Bacterial regulatory mechanisms for the control of complex cellular mechanisms (2023)
- Two years into COVID-19 pandemic: Where are we? (2022)
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Article
- Transposon insertion site sequencing (TIS) of Pseudomonas aeruginosa
- Hongbaek Cho
- J. Microbiol. 2021;59(12):1067-1074. Published online December 4, 2021
- DOI: https://doi.org/10.1007/s12275-021-1565-y
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- 7 Web of Science
- 7 Crossref
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Abstract
PDF - Transposon insertion site sequencing (TIS) is a technique that determines the insertion profile of a transposon mutant library by massive parallel sequencing of transposon-genomic DNA junctions. Because the transposon insertion profile reflects the abundance of each mutant in the library, it provides information to assess the fitness contribution of each genetic locus of a bacterial genome in a specific growth condition or strain background. Although introduced only about a dozen years ago, TIS has become an important tool in bacterial genetics that provides clues to study biological functions and regulatory mechanisms. Here, I describe a protocol for generating high density transposon insertion mutant libraries and preparing Illumina sequencing samples for mapping the transposon junctions of the transposon mutant libraries using Pseudomonas aeruginosa as an example.
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Citations
Citations to this article as recorded by
- Tn‐seq of Thermus thermophilus Genome Reveals Unexpected Tolerance to Insertions in Bacterial Common Essential Genes
Cristina L. Gómez‐Campo, Marc Gost, Bruna Fernanda Silva de Sousa, Laura Álvarez, José Berenguer, Modesto Redrejo‐Rodríguez, Mario Mencía
MicrobiologyOpen.2026;[Epub] CrossRef - Transposon insertion sequencing analysis reveals conditional essential genes for infection in Pseudomonas plecoglossicida
Haoyuan Ding, Peherden Ahat, Wei Song, Yuanxing Zhang, Sang Ho Choi, Yibei Zhang, Qiyao Wang
Aquaculture.2025; 607: 742655. CrossRef - mariner elements as a model for analyzing the stress response and somatic mobilization activity of transposable elements
Mariana Cancian, Fabiana Herédia, Alisson M. Gontijo, Elgion Lucio Silva Loreto
Heredity.2025; 134(12): 659. CrossRef - Optimizing phage-based mutant recovery and minimizing heat effect in the construction of transposon libraries in Staphylococcus aureus
Sally W. Yousief, Nader Abdelmalek, Bianca Paglietti
Scientific Reports.2024;[Epub] CrossRef - The biological essence of synthetic lethality: Bringing new opportunities for cancer therapy
Meiyi Ge, Jian Luo, Yi Wu, Guobo Shen, Xi Kuang
MedComm – Oncology.2024;[Epub] CrossRef - Optimization of Transposon Mutagenesis Methods in Pseudomonas antarctica
Sangha Kim, Changhan Lee
Microorganisms.2023; 11(1): 118. CrossRef - Construction of high-density transposon mutant library of Staphylococcus aureus using bacteriophage ϕ11
Wonsik Lee
Journal of Microbiology.2022; 60(12): 1123. CrossRef
- Tn‐seq of Thermus thermophilus Genome Reveals Unexpected Tolerance to Insertions in Bacterial Common Essential Genes
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