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- Note] Antifungal Chitinase against Human Pathogenic Yeasts from Coprinellus congregatus
- Yeeun Yoo Hyoung T. Choi
- J. Microbiol. 2014;52(5):441-443. Published online February 17, 2014
- DOI: https://doi.org/10.1007/s12275-014-3257-3
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- 2 Crossref
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Abstract
- The inky cap, Coprinellus congregatus, produces mushrooms which become autolyzed rapidly to generate black liquid droplets, in which no cell wall is detected by microscopy. A chitinase (Chi2) which is synthesized during the autolytic phase of C. congregatus inhibits the growths of Candida al-bicans and Cryptococcus neoformans up to 10% at the con-centration of 10 μg/ml, about 50% at concentration of 20 μg/ml, and up to 95% at the concentration of 70 μg/ml. Upon treatment these yeast cells are observed to be severely de-formed, with the formation of large holes in the cell wall. The two yeast species show no growth inhibition at the concen-tration of 5 μg/ml, which means the minimum inhibitory concentrations for both yeast species are 10 μg/ml under these experimental conditions.
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Analysis of the Antifungal Potential of
Macrocybe Titans
Extract Against
Candida Albicans
Fernanda CBN Pereira, Gabrielle C Peiter, Vivian EMS Justo, Gabrieli M Huff, Pollyanna CV Conrado, Mauro AP da Silva, Patrícia S Bonfim-Mendonça, Terezinha IE Svidzinski, Fabio R Rosado, Adriana Fiorini
Future Microbiology.2023; 18(6): 357. CrossRef -
Disarming Fungal Pathogens:
Bacillus safensis
Inhibits Virulence Factor Production and Biofilm Formation by
Cryptococcus neoformans
and
Candida albicans
François L. Mayer, James W. Kronstad, Yong-Sun Bahn, J. Andrew Alspaugh, Deborah Hogan
mBio.2017;[Epub] CrossRef
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Analysis of the Antifungal Potential of
Macrocybe Titans
Extract Against
Candida Albicans
Research Support, Non-U.S. Gov't
- Increase of Yeast Survival under Oxidative Stress by the Expression of the Laccase Gene from Coprinellus congregatus
- Dongsik Kim , Eunju Kwak , Hyoung T. Choi
- J. Microbiol. 2006;44(6):617-621.
- DOI: https://doi.org/2466 [pii]
- 37 View
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Abstract
- Coprinellus congregatus secreted a laccase isozyme when the culture was transferred to an acidic liquid medium (pH 4.1). The laccase cDNA gene (clac2) was used as a probe for cloning of the genomic laccase gene (lac2) including the promoter (Plac2). The open reading frame (ORF) of lac2 had 526 deduced amino acids and four conserved copper binding domains as other fungal laccases. Recombinant plasmid (pRSlac2p-cDNA) of lac2 cDNA with its own promoter was transformed in Saccharomyces cerevisiae. Expression of the transformed lac2 gene was induced by oxidative stress (H2O2) in yeast and the survival rate of the transformed yeast strain was greatly increased when compared with that of the control strain transformed with pRS316 yeast vector.
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