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- Hrq1 Facilitates Nucleotide Excision Repair of DNA Damage Induced by 4-Nitroquinoline-1-Oxide and Cisplatin in Saccharomyces cerevisiae
- Do-Hee Choi , Moon-Hee Min , Min-Ji Kim , Rina Lee , Sung-Hun Kwon , Sung-Ho Bae
- J. Microbiol. 2014;52(4):292-298. Published online March 29, 2014
- DOI: https://doi.org/10.1007/s12275-014-4018-z
- 51 View
- 0 Download
- 15 Crossref
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Abstract
- Hrq1 helicase is a novel member of the RecQ family. Among the five human RecQ helicases, Hrq1 is most homologous to RECQL4 and is conserved in fungal genomes. Recent genetic and biochemical studies have shown that it is a functional gene, involved in the maintenance of genome stability. To better define the roles of Hrq1 in yeast cells, we investigated genetic interactions between HRQ1 and several DNA repair genes. Based on DNA damage sensitivities induced by 4-nitroquinoline- 1-oxide (4-NQO) or cisplatin, RAD4 was found to be epistatic to HRQ1. On the other hand, mutant strains defective in either homologous recombination (HR) or postreplication repair (PRR) became more sensitive by additional deletion of HRQ1, indicating that HRQ1 functions in the RAD4-dependent nucleotide excision repair (NER) pathway independent of HR or PRR. In support of this, yeast twohybrid analysis showed that Hrq1 interacted with Rad4, which was enhanced by DNA damage. Overexpression of Hrq1K318A helicase-deficient protein rendered mutant cells more sensitive to 4-NQO and cisplatin, suggesting that helicase activity is required for the proper function of Hrq1 in NER.
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Citations
Citations to this article as recorded by
- Metabolomics Combined with Physiology and Transcriptomics Reveal the Response of Samsoniella hepiali to Key Metabolic Pathways and Its Degradation Mechanism during Subculture
Hui He, Zhengfei Cao, Tao Wang, Chuyu Tang, Yuling Li, Xiuzhang Li
Antioxidants.2024; 13(7): 780. CrossRef - Yeast Ribonucleotide Reductase Is a Direct Target of the Proteasome and Provides Hyper Resistance to the Carcinogen 4-NQO
Daria S. Spasskaya, Kirill A. Kulagin, Evgenia N. Grineva, Pamila J. Osipova, Svetlana V. Poddubko, Julia A. Bubis, Elizaveta M. Kazakova, Tomiris T. Kusainova, Vladimir A. Gorshkov, Frank Kjeldsen, Vadim L. Karpov, Irina A. Tarasova, Dmitry S. Karpov
Journal of Fungi.2023; 9(3): 351. CrossRef - Hrq1/RECQL4 regulation is critical for preventing aberrant recombination during DNA intrastrand crosslink repair and is upregulated in breast cancer
Thong T. Luong, Zheqi Li, Nolan Priedigkeit, Phoebe S. Parker, Stefanie Böhm, Kyle Rapchak, Adrian V. Lee, Kara A. Bernstein, Dmitry A. Gordenin
PLOS Genetics.2022; 18(9): e1010122. CrossRef - A skipping rope translocation mechanism in a widespread family of DNA repair helicases
Johann J Roske, Sunbin Liu, Bernhard Loll, Ursula Neu, Markus C Wahl
Nucleic Acids Research.2021; 49(1): 504. CrossRef - Role and Regulation of the RECQL4 Family during Genomic Integrity Maintenance
Thong T. Luong, Kara A. Bernstein
Genes.2021; 12(12): 1919. CrossRef - Zuo1 supports G4 structure formation and directs repair toward nucleotide excision repair
Alessio De Magis, Silvia Götz, Mona Hajikazemi, Enikő Fekete-Szücs, Marco Caterino, Stefan Juranek, Katrin Paeschke
Nature Communications.2020;[Epub] CrossRef - The Genetic and Physical Interactomes of theSaccharomyces cerevisiaeHrq1 Helicase
Cody M Rogers, Elsbeth Sanders, Phoebe A Nguyen, Whitney Smith-Kinnaman, Amber L Mosley, Matthew L Bochman
G3 Genes|Genomes|Genetics.2020; 10(12): 4347. CrossRef - Maintenance of Yeast Genome Integrity by RecQ Family DNA Helicases
Sonia Vidushi Gupta, Kristina Hildegard Schmidt
Genes.2020; 11(2): 205. CrossRef - Analyses of DNA double-strand break repair pathways in tandem arrays of HXT genes of Saccharomyces cerevisiae
Ju-Hee Choi, Ye-Seul Lim, Min-Ku Kim, Sung-Ho Bae
Journal of Microbiology.2020; 58(11): 957. CrossRef - DNA Helicases as Safekeepers of Genome Stability in Plants
Annika Dorn, Holger Puchta
Genes.2019; 10(12): 1028. CrossRef - The RecQ‐like helicase HRQ1 is involved in DNA crosslink repair in Arabidopsis in a common pathway with the Fanconi anemia‐associated nuclease FAN1 and the postreplicative repair ATPase RAD5A
Sarah Röhrig, Annika Dorn, Janina Enderle, Angelina Schindele, Natalie J. Herrmann, Alexander Knoll, Holger Puchta
New Phytologist.2018; 218(4): 1478. CrossRef - Yeast Hrq1 shares structural and functional homology with the disease-linked human RecQ4 helicase
Cody M. Rogers, Joseph Che-Yen Wang, Hiroki Noguchi, Tsuyoshi Imasaki, Yuichiro Takagi, Matthew L. Bochman
Nucleic Acids Research.2017; 45(9): 5217. CrossRef - Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells
Maciej Kliszczak, Hana Sedlackova, Ganesha P. Pitchai, Werner W. Streicher, Lumir Krejci, Ian D. Hickson
Oncotarget.2015; 6(38): 40464. CrossRef - DNA repair defects ascribed to pby1 are caused by disruption of Holliday junction resolvase Mus81-Mms4
Gizem Ölmezer, Dominique Klein, Ulrich Rass
DNA Repair.2015; 33: 17. CrossRef - Characterization of Hrq1-Rad14 Interaction in Saccharomyces cerevisiae
Moon-Hee Min, Min-Ji Kim, You-Jin Choi, Min-Ju You, Uy-Ra Kim, Hyo-Bin An, Chae-Hyun Kim, Chae-Yeon Kwon, Sung-Ho Bae
The Korean Journal of Microbiology.2014; 50(2): 95. CrossRef
- Metabolomics Combined with Physiology and Transcriptomics Reveal the Response of Samsoniella hepiali to Key Metabolic Pathways and Its Degradation Mechanism during Subculture
- Hrq1 Functions Independently of Sgs1 to Preserve Genome Integrity in Saccharomyces cerevisiae
- Do-Hee Choi , Rina Lee , Sung-Hun Kwon , Sung-Ho Bae
- J. Microbiol. 2013;51(1):105-112. Published online March 2, 2013
- DOI: https://doi.org/10.1007/s12275-013-3048-2
- 38 View
- 0 Download
- 18 Crossref
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Abstract
- Maintenance of genome stability in eukaryotes involves a number of conserved proteins, including RecQ helicases, which play multiple roles at various steps in homologous recombination and DNA repair pathways. Sgs1 has been described as the only RecQ helicase in lower eukaryotes. However, recent studies revealed the presence of a second RecQ helicase, Hrq1, which is most homologous to human RECQL4. Here we show that hrq1Δ mutation resulted in increased mitotic recombination and spontaneous mutation in Saccharomyces cerevisiae, and sgs1Δ mutation had additive effects on the phenotypes of hrq1Δ. We also observed that the hrq1Δ mutant was sensitive to 4-nitroquinoline 1-oxide and cisplatin, which was not complemented by overexpression of Sgs1. In addition, the hrq1Δ sgs1Δ double mutant displayed synthetic growth defect as well as a shortened chronological life span compared with the respective single mutants. Analysis of the type of age-dependent Canr mutations revealed that only point mutations were found in hrq1Δ, whereas significant numbers of gross deletion mutations were found in sgs1Δ. Our results suggest that Hrq1 is involved in recombination and DNA repair pathways in S. cerevisiae independent of Sgs1.
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Citations
Citations to this article as recorded by- Deletion of IRC19 Causes Defects in DNA Double-Strand Break Repair Pathways in Saccharomyces cerevisiae
Ju-Hee Choi, Oyungoo Bayarmagnai, Sung-Ho Bae
Journal of Microbiology.2024; 62(9): 749. CrossRef - Systematic analysis of changes across different developmental stages of the mushroom Sarcomyxa edulis
Chao Duan, Lan Yao, Jian-Hua Lv, Chuan-Wen Jia, Feng-hua Tian, Chang-Tian Li
Gene.2022; 824: 146450. CrossRef - Human RecQ Helicases in DNA Double-Strand Break Repair
Huiming Lu, Anthony J. Davis
Frontiers in Cell and Developmental Biology.2021;[Epub] CrossRef - Effects of the loss of mismatch repair genes on single-strand annealing between divergent sequences in Saccharomyces cerevisiae
Ye-Seul Lim, Ju-Hee Choi, Kyu-Jin Ahn, Min-Ku Kim, Sung-Ho Bae
Journal of Microbiology.2021; 59(4): 401. CrossRef - Analyses of DNA double-strand break repair pathways in tandem arrays of HXT genes of Saccharomyces cerevisiae
Ju-Hee Choi, Ye-Seul Lim, Min-Ku Kim, Sung-Ho Bae
Journal of Microbiology.2020; 58(11): 957. CrossRef - The Genetic and Physical Interactomes of theSaccharomyces cerevisiaeHrq1 Helicase
Cody M Rogers, Elsbeth Sanders, Phoebe A Nguyen, Whitney Smith-Kinnaman, Amber L Mosley, Matthew L Bochman
G3 Genes|Genomes|Genetics.2020; 10(12): 4347. CrossRef - Maintenance of Yeast Genome Integrity by RecQ Family DNA Helicases
Sonia Vidushi Gupta, Kristina Hildegard Schmidt
Genes.2020; 11(2): 205. CrossRef - The nature of meiotic chromosome dynamics and recombination in budding yeast
Soogil Hong, Jeong Hwan Joo, Hyeseon Yun, Keunpil Kim
Journal of Microbiology.2019; 57(4): 221. CrossRef - Ku complex suppresses recombination in the absence of MRX activity during budding yeast meiosis
Hyeseon Yun, Keunpil Kim
BMB Reports.2019; 52(10): 607. CrossRef - The RecQ‐like helicase HRQ1 is involved in DNA crosslink repair in Arabidopsis in a common pathway with the Fanconi anemia‐associated nuclease FAN1 and the postreplicative repair ATPase RAD5A
Sarah Röhrig, Annika Dorn, Janina Enderle, Angelina Schindele, Natalie J. Herrmann, Alexander Knoll, Holger Puchta
New Phytologist.2018; 218(4): 1478. CrossRef - Disruption of SUMO-targeted ubiquitin ligases Slx5–Slx8/RNF4 alters RecQ-like helicase Sgs1/BLM localization in yeast and human cells
Stefanie Böhm, Michael Joseph Mihalevic, Morgan Alexandra Casal, Kara Anne Bernstein
DNA Repair.2015; 26: 1. CrossRef - Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells
Maciej Kliszczak, Hana Sedlackova, Ganesha P. Pitchai, Werner W. Streicher, Lumir Krejci, Ian D. Hickson
Oncotarget.2015; 6(38): 40464. CrossRef - Hrq1 facilitates nucleotide excision repair of DNA damage induced by 4-nitroquinoline-1-oxide and cisplatin in Saccharomyces cerevisiae
Do-Hee Choi, Moon-Hee Min, Min-Ji Kim, Rina Lee, Sung-Hun Kwon, Sung-Ho Bae
Journal of Microbiology.2014; 52(4): 292. CrossRef - Conditional Genetic Interactions ofRTT107,SLX4, andHRQ1Reveal Dynamic Networks upon DNA Damage inS. cerevisiae
Grace P Leung, Maria J Aristizabal, Nevan J Krogan, Michael S Kobor
G3 Genes|Genomes|Genetics.2014; 4(6): 1059. CrossRef - Characterization of the Caenorhabditis elegans HIM-6/BLM Helicase: Unwinding Recombination Intermediates
Hana Jung, Jin A Lee, Seoyoon Choi, Hyunwoo Lee, Byungchan Ahn, Michael Lichten
PLoS ONE.2014; 9(7): e102402. CrossRef - Characterization of Hrq1-Rad14 Interaction in Saccharomyces cerevisiae
Moon-Hee Min, Min-Ji Kim, You-Jin Choi, Min-Ju You, Uy-Ra Kim, Hyo-Bin An, Chae-Hyun Kim, Chae-Yeon Kwon, Sung-Ho Bae
The Korean Journal of Microbiology.2014; 50(2): 95. CrossRef - The intrinsically disordered amino-terminal region of human RecQL4: multiple DNA-binding domains confer annealing, strand exchange and G4 DNA binding
Heidi Keller, Kristin Kiosze, Juliane Sachsenweger, Sebastian Haumann, Oliver Ohlenschläger, Tarmo Nuutinen, Juhani E. Syväoja, Matthias Görlach, Frank Grosse, Helmut Pospiech
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Deborah L. Croteau, Venkateswarlu Popuri, Patricia L. Opresko, Vilhelm A. Bohr
Annual Review of Biochemistry.2014; 83(1): 519. CrossRef
- Deletion of IRC19 Causes Defects in DNA Double-Strand Break Repair Pathways in Saccharomyces cerevisiae
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