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- Cryptic prophages in a blaNDM-1-bearing plasmid increase bacterial survival against high NaCl concentration, high and low temperatures, and oxidative and immunological stressors
- So Yeon Kim , Kwan Soo Ko
- J. Microbiol. 2020;58(6):483-488. Published online March 28, 2020
- DOI: https://doi.org/10.1007/s12275-020-9605-6
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- 5 Web of Science
- 4 Crossref
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Abstract
- In this study, we investigated the effect of cryptic prophage regions in a blaNDM-1-bearing plasmid, which was identified in a patient from South Korea, on the survival of bacteria against adverse environmental conditions. First, we conjugated the intact plasmid and plasmids with deleted cryptic prophages into Escherichia coli DH5α. The E. coli transconjugants carrying the plasmid with intact cryptic prophages showed increased survival during treatment with a high concentration of NaCl, high and low temperatures, an oxidative stressor (H2O2), and an immunological stressor (human serum). By contrast, the transconjugants carrying the plasmid with a single-cryptic prophage knockout did not show any change in survival rates. mRNA expression analyses revealed that the genes encoding sigma factor proteins were highly upregulated by the tested stressors and affected the expression of various proteins (antioxidant, cell osmosis-related, heat shock, cold shock, and universal stress proteins) associated with the specific defense against each stress. These findings indicate that a bacterial strain carrying a plasmid with intact carbapenemase gene and cryptic prophage regions exhibited an increased resistance against simulated environmental stresses, and cryptic prophages in the plasmid might contribute to this enhanced stress resistance. Our study indicated that the coselection of antibiotic resistance and resistance to other stresses may help bacteria to increase survival rates against adverse environments and disseminate.
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Citations
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- Uncovering the virome and its interaction with antibiotic resistome during compost fertilization
Qingxia Zhang, Lei Zhou, Yilong Zhao, Shuhong Gao, Yanjun Yang, Qingyun Chen, Wenhui Li, Qi Qi, Qiang Dong, Jiesi Lei, Xue Guo, Qun Gao, Yunfeng Yang
Journal of Hazardous Materials.2023; 457: 131763. CrossRef - Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef - Presence and Persistence of Putative Lytic and Temperate Bacteriophages in Vaginal Metagenomes from South African Adolescents
Anna-Ursula Happel, Christina Balle, Brandon S. Maust, Iyaloo N. Konstantinus, Katherine Gill, Linda-Gail Bekker, Rémy Froissart, Jo-Ann Passmore, Ulas Karaoz, Arvind Varsani, Heather Jaspan
Viruses.2021; 13(12): 2341. CrossRef - Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus
Jaejin Lee, Eunkyoung Shin, Jaeyeong Park, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(12): 1133. CrossRef
- Uncovering the virome and its interaction with antibiotic resistome during compost fertilization
Research Support, Non-U.S. Gov't
- Prediction of Bacterial Proteins Carrying A Nuclear Localization Signal and Nuclear Targeting of HsdM from Klebsiella pneumoniae
- Je Chul Lee , Dong Sun Kim , Dong Chan Moon , Jung-Hwa Lee , Mi Jin Kim , Su Man Lee , Yong Seok Lee , Se-Won Kang , Eun Jung Lee , Sang Sun Kang , Eunpyo Lee , Sung Hee Hyun
- J. Microbiol. 2009;47(5):641-645. Published online October 24, 2009
- DOI: https://doi.org/10.1007/s12275-009-0217-4
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- 15 Scopus
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Abstract
- Nuclear targeting of bacterial proteins is an emerging pathogenic mechanism whereby bacterial proteins can interact with nuclear molecules and alter the physiology of host cells. The fully sequenced bacterial genome can predict proteins that target the nuclei of host cells based on the presence of nuclear localization signal (NLS). In the present study, we predicted bacterial proteins with the NLS sequences from Klebsiella pneumoniae by bioinformatic analysis, and 13 proteins were identified as carrying putative NLS sequences. Among them, HsdM, a subunit of KpnAI that is a type I restriction-modification system found in K. pneumoniae, was selected for the experimental proof of nuclear targeting in host cells. HsdM carried the NLS sequences, 7KKAKAKK13, in the N-terminus. A transient expression of HsdM-EGFP in COS-1 cells exhibited exclusively a nuclear localization of the fusion proteins, whereas the fusion proteins of HsdM with substitutions in residues lysine to alanine in the NLS sequences, 7AAAKAAA13, were localized in the cytoplasm. HsdM was co-localized with importin α in the nuclei of host cells. Recombinant HsdM alone methylated the eukaryotic DNA in vitro assay. Although HsdM tested in this study has not been considered to be a virulence factor, the prediction of NLS motifs from the full sequenced genome of bacteria extends our knowledge of functional genomics to understand subcellular targeting of bacterial proteins.
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