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- PROTOCOL] Applications of different solvents and conditions for differential extraction of lipopolysaccharide in Gram-negative bacteria
- Mai Phuong Nguyen , Le Viet Ha Tran , Hyun Namgoong , Yong-Hak Kim
- J. Microbiol. 2019;57(8):644-654. Published online May 23, 2019
- DOI: https://doi.org/10.1007/s12275-019-9116-5
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- 6 Web of Science
- 6 Crossref
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Abstract
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Lipopolysaccharide (LPS) is one of the major components in
the outer membrane of Gram-negative bacteria. However,
its heterogeneity and variability in different bacteria and differentiation
conditions make it difficult to extract all of the
structural variants. We designed a solution to improve quality
and biological activity of LPS extracted from various bacteria
with different types of LPS, as compared to conventional
methods
. We introduced a quality index as a simple measure of LPS purity in terms of a degree of polysaccharide content detected by absorbance at 204 nm. Further experiments using gel electrophoresis, endotoxin test, and macrophage activation test were performed to evaluate the performance and reliability of a proposed ‘T-sol’ method and the biological effectiveness and character of the LPS products. We presented that the T-sol method had differential effects on extraction of a RAW 264.7 cell-activating LPS, which was effective in the macrophage activation with similar effects in stimulating the production of TNF-alpha. In conclusion, the T-sol method provides a simple way to improve quality and biological activity of LPS with high yield. -
Citations
Citations to this article as recorded by
- Effective Modalities of Periodontitis Induction in Rat Model
Fazle Khuda, Badiah Baharin, Nur Najmi Mohamad Anuar, Bellen Sharon Fred Satimin, Nurrul Shaqinah Nasruddin
Journal of Veterinary Dentistry.2024; 41(1): 49. CrossRef - LPS-Induced Mortality in Zebrafish: Preliminary Characterisation of Common Fish Pathogens
Rafaela A. Santos, Cláudia Cardoso, Neide Pedrosa, Gabriela Gonçalves, Jorge Matinha-Cardoso, Filipe Coutinho, António P. Carvalho, Paula Tamagnini, Aires Oliva-Teles, Paulo Oliveira, Cláudia R. Serra
Microorganisms.2023; 11(9): 2205. CrossRef - Heterogeneity of Lipopolysaccharide as Source of Variability in Bioassays and LPS-Binding Proteins as Remedy
Alexandra C. Fux, Cristiane Casonato Melo, Sara Michelini, Benjamin J. Swartzwelter, Andreas Neusch, Paola Italiani, Martin Himly
International Journal of Molecular Sciences.2023; 24(9): 8395. CrossRef - Identification workflow of endotoxins by pyrolysis–gas chromatography–mass spectrometry based on a database and chemometrics
Jackie Jackie, Chun Kiang Chua, Norrapat Shih, Sam Fong Yau Li
Journal of Analytical and Applied Pyrolysis.2022; 165: 105547. CrossRef - Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis
Julian Aldana, Adriana Romero-Otero, Mónica P. Cala
Metabolites.2020; 10(6): 231. CrossRef - The outer membrane glycolipids of bacteria from cold environments: isolation, characterization, and biological activity
Angela Casillo, Ermenegilda Parrilli, Maria Luisa Tutino, Maria Michela Corsaro
FEMS Microbiology Ecology.2019;[Epub] CrossRef
- Effective Modalities of Periodontitis Induction in Rat Model
Research Support, Non-U.S. Gov't
- NOTE] Pontibacter jeungdoensis sp. nov., Isolated from a Solar Saltern in Korea
- Yochan Joung , Haneul Kim , Beom-Il Lee , Heeyoung Kang , Tae Yong Jang , O-Seob Kwon , Kiseong Joh
- J. Microbiol. 2013;51(4):531-535. Published online August 30, 2013
- DOI: https://doi.org/10.1007/s12275-013-2324-5
- 39 View
- 0 Download
- 12 Crossref
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Abstract
- A Gram-staining-negative, rod-shaped and red-pigmented bacterial strain, HMD3125T, was isolated from a solar saltern in Jeungdo, Republic of Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD3125T formed a lineage within the genus Pontibacter and was similar to Pontibacter salisaro (96.1%) and P. korlensis (95.3%). The major fatty acids of strain HMD3125T were summed feature 4 (comprising iso-C17:1 I and/or anteiso-C17:1 B; 30.4%), iso-C15:0 (20.4%) and iso-C17:0 3OH (17.2%). The polar lipid profile of HMD3125T consisted of the phosphatidylethanolamine, four unidentified polar lipids, unidentified phospholipid, unidentified aminolipid and unidentified aminophospholipid. Strain HMD3125T contained MK-7 as the predominant menaquinone and sym-homospermidine as the major polyamine. The DNA G+C content of strain HMD3125T was 45.6 mol%. Strain HMD3125T assigned as a novel species in the genus Pontibacter, for which the name Pontibacter jeungdoensis sp. nov. is proposed. The type strain is HMD3125T (=KCTC 23156T =CECT 7710T).
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Citations
Citations to this article as recorded by- Microbial community and functional prediction during the processing of salt production in a 1000-year-old marine solar saltern of South China
Ya-Li Wei, Zi-Jie Long, Ming-Xun Ren
Science of The Total Environment.2022; 819: 152014. CrossRef -
Pontibacter aquaedesilientis sp. nov., isolated from Jeongbang Waterfall, Jeju Island
Geeta Chhetri, Taegun Seo
International Journal of Systematic and Evolutionary Microbiology .2021;[Epub] CrossRef - High-throughput absolute quantification sequencing reveals the effect of different fertilizer applications on bacterial community in a tomato cultivated coastal saline soil
Si-Qi Jiang, Ya-Nan Yu, Ren-Wei Gao, Huan Wang, Jian Zhang, Rong Li, Xiao-Hua Long, Qi-Rong Shen, Wei Chen, Feng Cai
Science of The Total Environment.2019; 687: 601. CrossRef - Pontibacter chitinilyticus sp. nov., a novel chitin-hydrolysing bacterium isolated from soil
Geeta Chhetri, Jiyoun Kim, Inhyup Kim, Myung Kyum Kim, Taegun Seo
Antonie van Leeuwenhoek.2019; 112(7): 1011. CrossRef - Pontibacter locisalis Sy30T sp. nov. isolated from soil collected from an abandoned saltern
Yan-Xia Zhou, Zhi-Hong Xie, Jin-Xin Zhao, Zong-Jun Du, Guan-Jun Chen
Antonie van Leeuwenhoek.2016; 109(3): 415. CrossRef - Pontibacter amylolyticus sp. nov., isolated from a deep-sea sediment hydrothermal vent field
Yue-Hong Wu, Peng Zhou, Shu-Ling Jian, Zhen-Sheng Liu, Chun-Sheng Wang, Aharon Oren, Xue-Wei Xu
International Journal of Systematic and Evolutionary Microbiology.2016; 66(4): 1760. CrossRef -
Pontibacter aydingkolensis sp. nov., isolated from soil of a salt lake
Ghenijan Osman, Tao Zhang, Kai Lou, Yan Gao, Wei Chang, Qing Lin, Hong-Mei Yang, Xiang-Dong Huo, Ning Wang
International Journal of Systematic and Evolutionary Microbiology .2016; 66(12): 5523. CrossRef - Pontibacter mucosus sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment
Namita Nayyar, Puneet Kohli, Nitish Kumar Mahato, Rup Lal
International Journal of Systematic and Evolutionary Microbiology.2016; 66(6): 2234. CrossRef - Pontibacter ummariensis sp. nov., isolated from a hexachlorocyclohexane-contaminated soil
N. K. Mahato, C. Tripathi, N. Nayyar, A. K. Singh, R. Lal
International Journal of Systematic and Evolutionary Microbiology.2016; 66(2): 1080. CrossRef -
Pontibacter chinhatensis sp. nov., isolated from pond sediment containing discarded hexachlorocyclohexane isomer waste
Amit Kumar Singh, Nidhi Garg, Rup Lal
International Journal of Systematic and Evolutionary Microbiology .2015; 65(Pt_7): 2248. CrossRef - Pontibacter humi sp. nov., Isolated from Mountain Soil
Sathiyaraj Srinivasan, Jae-Jin Lee, Sang-Seob Lee, Myung Kyum Kim
Current Microbiology.2014; 69(3): 263. CrossRef - Pontibacter diazotrophicus sp. nov., a Novel Nitrogen-Fixing Bacterium of the Family Cytophagaceae
Linghua Xu, Xian-Chun Zeng, Yao Nie, Xuesong Luo, Enmin Zhou, Lingli Zhou, Yunfan Pan, Wenjun Li, Paul Jaak Janssen
PLoS ONE.2014; 9(3): e92294. CrossRef
- Microbial community and functional prediction during the processing of salt production in a 1000-year-old marine solar saltern of South China
Journal Article
- Optimization of Lactic Acid Production in SSF by Lactobacillus amylovorus NRRL B-4542 Using Taguchi Methodology
- Pyde Acharya Nagarjun , Ravella Sreenivas Rao , Swargam Rajesham , Linga Venkateswar Rao
- J. Microbiol. 2005;43(1):38-43.
- DOI: https://doi.org/2140 [pii]
- 51 View
- 0 Download
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Abstract
- Lactic acid production parameter optimization using Lactobacillus amylovorus NRRL B-4542 was performed using the design of experiments (DOE) available in the form of an orthogonal array and a software for automatic design and analysis of the experiments, both based on Taguchi protocol. Optimal levels of physical parameters and key media components namely temperature, pH, inoculum size, moisture, yeast extract, MgSO_4 . 7H_20, Tween 80, and corn steep liquor (CSL) were determined. Among the physical parameters, temperature contributed higher influence, and among media components, yeast extract, MgSO_4 . 7H_20, and Tween 80 played important roles in the conversion of starch to lactic acid. The expected yield of lactic acid under these optimal conditions was 95.80% and the actual yield at optimum conditions was 93.50%.
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