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- Characteristic and role of chromosomal type II toxin-antitoxin systems locus in Enterococcus faecalis ATCC29212
- Zhen Li , Chao Shi , Shanjun Gao , Xiulei Zhang , Di Lu , Guangzhi Liu
- J. Microbiol. 2020;58(12):1027-1036. Published online October 23, 2020
- DOI: https://doi.org/10.1007/s12275-020-0079-3
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- 4 Web of Science
- 3 Crossref
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Abstract
- The Gram-positive bacterium Enterococcus faecalis is currently one of the major pathogens of nosocomial infections. The lifestyle of E. faecalis relies primarily on its remarkable capacity to face and survive in harsh environmental conditions. Toxin-antitoxin (TA) systems have been linked to the growth control of bacteria in response to adverse environments but have rarely been reported in Enterococcus. Three functional type II TA systems were identified among the 10 putative TA systems encoded by E. faecalis ATCC29212. These toxin genes have conserved domains homologous to MazF (DR75_ 1948) and ImmA/IrrE family metallo-endopeptidases (DR75_ 1673 and DR75_2160). Overexpression of toxin genes could inhibit the growth of Escherichia coli. However, the toxin DR75_1673 could not inhibit bacterial growth, and the bacteriostatic effect occurred only when it was coexpressed with the antitoxin DR75_1672. DR75_1948–DR75_1949 and DR75_ 160–DR75_2161 could maintain the stable inheritance of the unstable plasmid pLMO12102 in E. coli. Moreover, the transcription levels of these TAs showed significant differences when cultivated under normal conditions and with different temperatures, antibiotics, anaerobic agents and H2O2. When DR75_2161 was knocked out, the growth of the mutant strain at high temperature and oxidative stress was limited. The experimental characterization of these TAs loci might be helpful to investigate the key roles of type II TA systems in the physiology and environmental stress responses of Enterococcus.
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Citations
Citations to this article as recorded by
- Sonic-assisted antibacterial photodynamic therapy: a strategy for enhancing lateral canal disinfection
Yanhuang Wang, Lishan Lei, Jing Huang, Zhiyu Cai, Xiaojing Huang
BMC Oral Health.2024;[Epub] CrossRef - Unveiling the impact of antibiotic stress on biofilm formation and expression of toxin-antitoxin system genes in Clostridium difficile clinical isolates
Nasim Cheraghi, Saeed Khoshnood, Nourkhoda Sadeghifard, Niloufar Khodaei, Parisa Asadollahi, Saiyad Bastaminejad, Ebrahim Kouhsari, Nazanin Omidi, Behrooz Sadeghi Kalani
Molecular Biology Reports.2024;[Epub] CrossRef - Edwardsiella piscicida HigB: A type II toxin that is essential to oxidative resistance, biofilm formation, serum survival, intracellular propagation, and host infection
Jinhong Xie, Qianyun Zhao, Huiqin Huang, Zaiguang Fang, Yonghua Hu
Aquaculture.2021; 535: 736382. CrossRef
- Sonic-assisted antibacterial photodynamic therapy: a strategy for enhancing lateral canal disinfection
Research Support, Non-U.S. Gov't
- Molecular Detection and Characterization of Human Enteroviruses in Korean Surface Water
- Gyucheol Lee , Chanhee Lee
- J. Microbiol. 2008;46(3):319-324. Published online July 5, 2008
- DOI: https://doi.org/10.1007/s12275-007-0232-2
- 37 View
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- 8 Scopus
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Abstract
- In this study, the genetic epidemiology of enteroviruses (EVs) in Korean surface water was evaluated by conducting phylogenetic analyses of the nucleotide sequences of the 5’ non-coding region (5’ NCR), which was determined by RT-PCR analysis of total culturable virus assay-positive samples. The results showed that the nucleotide sequences of the EVs could be classified into 4 genetic clusters, and that the predominant presence of Korea EVs were very similar to echoviruses type 30. Interestingly, two nucleotide sequences were very similar to those of coxsackievirus type B1 isolated from aseptic meningitis patients in Seoul, Korea, implying the possibility of a common source for the viruses circulated in water systems and humans. In addition, 3 nucleotide sequences clustered strongly with the nucleotide sequences from China or Japan, and one fell into the same cluster as echovirus type 11 from Taiwan, which suggests that EVs in Asia may have evolved in a region-specific manner. Taken together, the results of this study revealed that EVs from Korea surface waters could be genetically classified as coxsackieviruses or echoviruses, and that they evolved in Asia in a region-specific manner.
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