Vibrio parahaemolyticus possesses two types of flagella: a
single polar flagellum (Pof) for swimming and the peritrichous
lateral flagella (Laf) for swarming. Expression of Laf
genes has previously been reported to be regulated by the quorum
sensing (QS) regulators AphA and OpaR. In the present
study, we showed that OpaR, the QS regulator at high cell density
(HCD), acted as a negative regulator of swimming motility
and the transcription of Pof genes in V. parahaemolyticus.
OpaR bound to the promoter-proximal DNA regions
of flgAMN, flgMN, and flgBCDEFGHIJ within the Pof gene
loci to repress their transcription, whereas it negatively regulates
the transcription of flgKL-flaC in an indirect manner.
Thus, this work investigated how QS regulated the swimming
motility via direct action of its master regulator OpaR on
the transcription of Pof genes in V. parahaemolyticus.
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