Journal of Microbiology

Journal Articles

Lipoteichoic Acid from Lacticaseibacillus rhamnosus GG as a Novel Intracanal Medicament Targeting Enterococcus faecalis Biofilm Formation: Ji-Young Yoon, Somin Park, Dongwook Lee, Ok-Jin Park, WooCheol Lee, Seung Hyun Han; J. Microbiol. 2024;62(10):897-905. Published online September 30, 2024; DOI: https://doi.org/10.1007/s12275-024-00165-6

41 View
0 Download

Abstract: The demand for safe and effective endodontic medicaments to control Enterococcus faecalis biofilms, a contributor to apical periodontitis, is increasing. Recently, lipoteichoic acid (LTA) of family Lactobacillaceae has been shown to have anti-biofilm effects against various oral pathogens. Preliminary experiments showed that LTA purified from Lacticaseibacillus rhamnosus GG (Lgg.LTA) was the most effective against E. faecalis biofilms among LTAs from three Lactobacillaceae including L. rhamnosus GG, Lacticaseibacillus casei, and Lactobacillus acidophilus. Therefore, in this study, we investigated the potential of Lgg.LTA as an intracanal medicament in human root canals infected with E. faecalis. Twenty eight dentinal cylinders were prepared from extracted human teeth, where two-week-old E. faecalis biofilms were formed followed by intracanal treatment with sterile distilled water (SDW), N-2 methyl pyrrolidone (NMP), calcium hydroxide (CH), or Lgg.LTA. Bacteria and biofilms that formed in the root canals were analyzed by scanning electron microscopy and confocal laser scanning microscopy. The remaining E. faecalis cells in the root canals after intracanal medicament treatment were enumerated by culturing and counting. When applied to intracanal biofilms, Lgg.LTA effectively inhibited E. faecalis biofilm formation as much as CH, while SDW and NMP had little effect. Furthermore, Lgg.LTA reduced both live and dead bacteria within the dentinal tubules, indicating the possibility of minimal re-infection in the root canals. Collectively, intracanal application of Lgg.LTA effectively inhibited E. faecalis biofilm formation, implying that Lgg.LTA can be used as a novel endodontic medicament.

Enterococcus Phage vB_EfaS_HEf13 as an Anti-Biofilm Agent Against Enterococcus faecalis: Dongwook Lee, Jintaek Im, A Reum Kim, Woohyung Jun, Cheol-Heui Yun, Seung Hyun Han; J. Microbiol. 2024;62(8):683-693. Published online June 27, 2024; DOI: https://doi.org/10.1007/s12275-024-00150-z

56 View
0 Download
1 Web of Science
1 Scopus

Abstract: Enterococcus faecalis is a Gram-positive bacterium that is frequently found in the periapical lesion of patients with apical periodontitis. Its biofilm formation in root canal is closely related to the development of refractory apical periodontitis by providing increased resistance to endodontic treatments. Phage therapy has recently been considered as an efficient therapeutic strategy in controlling various periodontal pathogens. We previously demonstrated the bactericidal capacities of Enterococcus phage vB_EfaS_HEf13 (phage HEf13) against clinically-isolated E. faecalis strains. Here, we investigated whether phage HEf13 affects biofilm formation and pre-formed biofilm of clinically-isolated E. faecalis, and its combinatory effect with endodontic treatments, including chlorhexidine (CHX) and penicillin. The phage HEf13 inhibited biofilm formation and disrupted pre-formed biofilms of E. faecalis in a dose- and time-dependent manner. Interestingly, phage HEf13 destroyed E. faecalis biofilm exopolysaccharide (EPS), which is known to be a major component of bacterial biofilm. Furthermore, combined treatment of phage HEf13 with CHX or penicillin more potently inhibited biofilm formation and disrupted pre-formed biofilm than either treatment alone. Confocal laser scanning microscopic examination demonstrated that these additive effects of the combination treatments on disruption of pre-formed biofilm are mediated by relatively enhanced reduction in thickness distribution and biomass of biofilm. Collectively, our results suggest that the effect of phage HEf13 on E. faecalis biofilm is mediated by its EPS-degrading property, and its combination with endodontic treatments more potently suppresses E. faecalis biofilm, implying that phage HEf13 has potential to be used as a combination therapy against E. faecalis infections.

Functional Characterization of DNA N‑Glycosylase Ogg1 and Ntg1 in DNA Damage Stress of Cryptococcus neoformans: Kwang-Woo Jung , Sunhak Kwon , Jong-Hyun Jung , Sangyong Lim , Yong-Sun Bahn; J. Microbiol. 2023;61(11):981-992. Published online December 6, 2023; DOI: https://doi.org/10.1007/s12275-023-00092-y

39 View
0 Download

Abstract: Reactive oxygen species induce DNA strand breaks and DNA oxidation. DNA oxidation leads to DNA mismatches, resulting in mutations in the genome if not properly repaired. Homologous recombination (HR) and non-homologous end-joining (NHEJ) are required for DNA strand breaks, whereas the base excision repair system mainly repairs oxidized DNAs, such as 8-oxoguanine and thymine glycol, by cleaving the glycosidic bond, inserting correct nucleotides, and sealing the gap. Our previous studies revealed that the Rad53-Bdr1 pathway mainly controls DNA strand breaks through the regulation of HRand NHEJ-related genes. However, the functional roles of genes involved in the base excision repair system remain elusive in Cryptococcus neoformans. In the present study, we identified OGG1 and NTG1 genes in the base excision repair system of C. neoformans, which are involved in DNA oxidation repair. The expression of OGG1 was induced in a Hog1-dependent manner under oxidative stress. On the other hand, the expression of NTG1 was strongly induced by DNA damage stress in a Rad53-independent manner. We demonstrated that the deletion of NTG1, but not OGG1, resulted in elevated susceptibility to DNA damage agents and oxidative stress inducers. Notably, the ntg1Δ mutant showed growth defects upon antifungal drug treatment. Although deletion of OGG1 or NTG1 did not increase mutation rates, the mutation profile of each ogg1Δ and ntg1Δ mutant was different from that of the wild-type strain. Taken together, we found that DNA N-glycosylase Ntg1 is required for oxidative DNA damage stress and antifungal drug resistance in C. neoformans.

Effects of Phosphorus‑dissolving Dark Septate Endophytes on the Growth of Blueberry: Qixin Luo , Rui Hou , Xiaojing Shang , Si Li; J. Microbiol. 2023;61(9):837-851. Published online October 5, 2023; DOI: https://doi.org/10.1007/s12275-023-00080-2

53 View
0 Download
2 Web of Science
2 Crossref

Abstract

Dark septate endophytes (DSEs) are widely distributed and improve plant growth. DSEs secrete large amounts of enzymes to mineralize insoluble phosphorus in soil and convert it into soluble phosphorus, promoting plant uptake of phosphorus. However, the effects of DSEs with phosphate-solubilizing ability on host plants need further study. In this study, phosphorusdissolving DSEs were screened for growth-promoting effects. We isolated, identified and characterized three DSE species (Thozetella neonivea, Pezicula ericae and Hyaloscyphaceae sp.) showing phosphate-solubilizing ability. The impact of single, dual or triple inoculation of DSEs on blueberry plant characteristics was studied. Their effects on colonization intensity, seedling biomass, nutrients in plants and soil, and activities of plant resistance enzymes and soil enzymes were markedly upregulated relative to the control (P < 0.05). The available phosphorus and acid phosphatase levels in different combinations were significantly increased. These findings indicate that the application of the three DSEs may be valuable in facilitating the cultivation of blueberry with a higher biomass and improved plant quality.

Citations

Citations to this article as recorded by

Diversity and Functional Roles of Root-Associated Endophytic Fungi in Two Dominant Pioneer Trees Reclaimed from a Metal Mine Slag Heap in Southwest China
Bo Bi, Yuqing Xiao, Xiaonan Xu, Qianqian Chen, Haiyan Li, Zhiwei Zhao, Tao Li
Microorganisms.2024; 12(10): 2067. CrossRef
Short-term organic fertilizer substitution increases sorghum yield by improving soil physicochemical characteristics and regulating microbial community structure
Mengen Nie, Guangqian Yue, Lei Wang, Yizhong Zhang
Frontiers in Plant Science.2024;[Epub] CrossRef

Thioredoxin A of Streptococcus suis Serotype 2 Contributes to Virulence by Inhibiting the Expression of Pentraxin 3 to Promote Survival Within Macrophages: Chijun Zhao , Xinglin Jia , Yanying Pan , Simeng Liao , Shuo Zhang , Chunxiao Ji , Guangwei Kuang , Xin Wu , Quan Liu , Yulong Tang , Lihua Fang; J. Microbiol. 2023;61(4):433-448. Published online April 3, 2023; DOI: https://doi.org/10.1007/s12275-023-00038-4

54 View
0 Download
2 Web of Science
2 Crossref

Abstract

Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that can infect humans in contact with infected pigs or their byproducts. It can employ different types of genes to defend against oxidative stress and ensure its survival. The thioredoxin (Trx) system is a key antioxidant system that contributes adversity adaptation and pathogenicity. SS2 has been shown to encode putative thioredoxin genes, but the biological roles, coding sequence, and underlying mechanisms remains uncharacterized. Here, we demonstrated that SSU05_0237-ORF, from a clinical SS2 strain, ZJ081101, encodes a protein of 104 amino acids with a canonical CGPC active motif and an identity 70–85% similar to the thioredoxin A (TrxA) in other microorganisms. Recombinant TrxA efficiently catalyzed the thiol-disulfide oxidoreduction of insulin. The deletion of TrxA led to a significantly slow growth and markedly compromised tolerance of the pathogen to temperature stress, as well as impaired adhesion ability to pig intestinal epithelial cells (IPEC-J2). However, it was not involved in H2O2 and paraquat-induced oxidative stress. Compared with the wild-type strain, the ΔTrxA strain was more susceptible to killing by macrophages through increasing NO production. Treatment with TrxA mutant strain also significantly attenuated cytotoxic effects on RAW 264.7 cells by inhibiting inflammatory response and apoptosis. Knockdown of pentraxin 3 in RAW 264.7 cells was more vulnerable to phagocytic activity, and TrxA promoted SS2 survival in phagocytic cells depending on pentraxin 3 activity compared with the wild-type strain. Moreover, a co-inoculation experiment in mice revealed that TrxA mutant strain is far more easily cleared from the body than the wild type strain in the period from 8–24 h, and exhibits significantly attenuated oxidative stress and liver injury. In summary, we reveal the important role of TrxA in the pathogenesis of SS2.

Citations

Citations to this article as recorded by

Thioredoxin C of Streptococcus suis serotype 2 contributes to virulence by inducing antioxidative stress and inhibiting autophagy via the MSR1/PI3K-Akt-mTOR pathway in macrophages
Chunxiao Ji, Yanying Pan, Bocheng Liu, Jianying Liu, Chijun Zhao, Zhuyuan Nie, Simeng Liao, Guangwei Kuang, Xin Wu, Quan Liu, Jie Ning, Yulong Tang, Lihua Fang
Veterinary Microbiology.2024; 298: 110263. CrossRef
A Comprehensive Review on the Roles of Metals Mediating Insect–Microbial Pathogen Interactions
Subhanullah Khan, Minglin Lang
Metabolites.2023; 13(7): 839. CrossRef

Lactobacillus plantarum lipoteichoic acid disrupts mature Enterococcus faecalis biofilm: A Reum Kim , Minji Kang , Yeon-Jee Yoo , Cheol-Heui Yun , Hiran Perinpanayagam , Kee-Yeon Kum , Seung Hyun Han; J. Microbiol. 2020;58(4):314-319. Published online January 28, 2020; DOI: https://doi.org/10.1007/s12275-020-9518-4

44 View
0 Download
18 Web of Science
19 Crossref

Abstract

Apical periodontitis is caused by biofilm-mediated root canal infection. Early phase oral bacterial biofilms are inhibited by Lactobacillus plantarum lipoteichoic acid (Lp.LTA). However, mature biofilms that develop over 3 weeks are more resistant to traditional endodontic medicaments. Therefore, this study examined the effectiveness of Lp.LTA on disrupting mature Enterococcus faecalis biofilms, and on enhancing the effects of endodontic medicaments. LTA was purified from L. plantarum through butanol extraction followed by hydrophobic and ion-exchange chromatography. E. faecalis biofilms were formed over 3 weeks on glass bottom dishes and in dentin blocks obtained from human single-rooted premolars. These mature biofilms were treated with or without Lp.LTA for 1 h, followed by additional treatment with either chlorhexidine digluconate (CHX), calcium hydroxide (CH), or triple antibiotics for 24 h. Biofilms on glass were live/dead stained and quantified by ZEN through confocal laser microscopy. Biofilms in dentin were fixed, sputter coated and analyzed by ImageJ with scanning electron microscopy. Preformed E. faecalis mature biofilms on the culture dishes were dose-dependently disrupted by Lp.LTA. Lp.LTA potentiated the effects of CHX or CH on the disruption of mature biofilm. Interestingly, CHX-induced disruption of preformed E. faecalis mature biofilms was synergistically enhanced only when pretreated with Lp.LTA. Furthermore, in the dentin block model, Lp.LTA alone reduced E. faecalis mature biofilm and pre-treatment with Lp.LTA promoted the anti-biofilm activity of CHX. Lp.LTA could be an anti-biofilm or supplementary agent that can be effective for E. faecalis-biofilminduced diseases.

Citations

Citations to this article as recorded by

A Systematic Review of the Comparative Efficacy of Lactobacillus Probiotics and Sodium Hypochlorite as Intracanal Irrigants Against Enterococcus faecalis
Mrinalini Mrinalini, Alpa Gupta, Dax Abraham, Arun Kumar Duraisamy, Rajat Sharma
Cureus.2024;[Epub] CrossRef
The role of Lactobacillus plantarum in oral health: a review of current studies
Xinyan Huang, Jianhang Bao, Mingzhen Yang, Yingying Li, Youwen Liu, Yuankun Zhai
Journal of Oral Microbiology.2024;[Epub] CrossRef
Lipoteichoic Acid from Lacticaseibacillus rhamnosus GG as a Novel Intracanal Medicament Targeting Enterococcus faecalis Biofilm Formation
Ji-Young Yoon, Somin Park, Dongwook Lee, Ok-Jin Park, WooCheol Lee, Seung Hyun Han
Journal of Microbiology.2024; 62(10): 897. CrossRef
Isolation, Identification and Antibacterial Characteristics of Lacticaseibacillus rhamnosus YT
Chengran Guan, Feng Li, Peng Yu, Xuan Chen, Yongqi Yin, Dawei Chen, Ruixia Gu, Chenchen Zhang, Bo Pang
Foods.2024; 13(17): 2706. CrossRef
Restriction of growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria
Prerna Saini, Repally Ayyanna, Rishi Kumar, Sayan Kumar Bhowmick, Vinay Bhaskar, Bappaditya Dey
Frontiers in Microbiology.2024;[Epub] CrossRef
Enterococcus Phage vB_EfaS_HEf13 as an Anti-Biofilm Agent Against Enterococcus faecalis
Dongwook Lee, Jintaek Im, A Reum Kim, Woohyung Jun, Cheol-Heui Yun, Seung Hyun Han
Journal of Microbiology.2024; 62(8): 683. CrossRef
Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model
Shymaa Shaaban, Salma Genena, Alaaeldin Elraggal, Gamal M. Hamad, Marwa A. Meheissen, Sybel Moussa
BMC Oral Health.2023;[Epub] CrossRef
Antioxidant Effects and Probiotic Properties of Latilactobacillus sakei MS103 Isolated from Sweet Pickled Garlic
Heng Li, Changlin Chen, Yuanxin Li, Zhengqiang Li, Chen Li, Chang Luan
Foods.2023; 12(23): 4276. CrossRef
Effectiveness of probiotics in apical periodontitis progression: A scoping review and implications for research
Giusy Rita Maria La Rosa, Eugenio Pedullà
Australian Endodontic Journal.2023; 49(S1): 528. CrossRef
Antibacterial efficacy of antibiotic pastes versus calcium hydroxide intracanal dressing: A systematic review and meta-analysis of ex vivo studies
Mohammadreza Vatankhah, Kamyar Khosravi, Nazanin Zargar, Armin Shirvani, MohammadHossein Nekoofar, Omid Dianat
Journal of Conservative Dentistry.2022; 25(5): 463. CrossRef
Plantaricin LD1 purified from Lactobacillus plantarum LD1 inhibits biofilm formation of Enterococcus faecalis ATCC 29212 in tooth model
M.K. Yadav, P. Yadav, M. Dhiman, S. Tewari, S.K. Tiwari
Letters in Applied Microbiology.2022; 75(3): 623. CrossRef
The Products of Probiotic Bacteria Effectively Treat Persistent Enterococcus faecalis Biofilms
Shatha Safadi, Harsh Maan, Ilana Kolodkin-Gal, Igor Tsesis, Eyal Rosen
Pharmaceutics.2022; 14(4): 751. CrossRef
Regenerative Endodontics and Minimally Invasive Dentistry: Intertwining Paths Crossing Over Into Clinical Translation
Hisham Elnawam, Menatallah Abdelmougod, Ahmed Mobarak, Mai Hussein, Hamdy Aboualmakarem, Michael Girgis, Rania El Backly
Frontiers in Bioengineering and Biotechnology.2022;[Epub] CrossRef
Can Probiotics Emerge as Effective Therapeutic Agents in Apical Periodontitis? A Review
Gaurav Kumar, Sanjay Tewari, John Tagg, Michael Leonidas Chikindas, Igor V Popov, Santosh Kumar Tiwari
Probiotics and Antimicrobial Proteins.2021; 13(2): 299. CrossRef
Interplay between Candida albicans and Lactic Acid Bacteria in the Gastrointestinal Tract: Impact on Colonization Resistance, Microbial Carriage, Opportunistic Infection, and Host Immunity
Karen D. Zeise, Robert J. Woods, Gary B. Huffnagle
Clinical Microbiology Reviews.2021;[Epub] CrossRef
Mucosal Bacteria Modulate Candida albicans Virulence in Oropharyngeal Candidiasis
M. Bertolini, R. Vazquez Munoz, L. Archambault, S. Shah, J. G. S. Souza, R. C. Costa, A. Thompson, Y. Zhou, T. Sobue, A. Dongari-Bagtzoglou, Deborah A. Hogan
mBio.2021;[Epub] CrossRef
Gram Positive Bacterial Lipoteichoic Acid Role in a Root Canal Infection – A Literature Review
Vinoo Subramaniam Ramachandran, Mensudar Rathakrishnan, Malathy Balaraman Ravindrran, Alargarsamy Venkatesh, Vidhya Shankari Shanmugasundaram, Karpagavinayagam Kumaraguru
Journal of Pure and Applied Microbiology.2021; 15(2): 534. CrossRef
Lactobacillus plantarum Lipoteichoic Acids Possess Strain-Specific Regulatory Effects on the Biofilm Formation of Dental Pathogenic Bacteria
Dongwook Lee, Jintaek Im, Dong Hyun Park, Sungho Jeong, Miri Park, Seokmin Yoon, Jaewoong Park, Seung Hyun Han
Frontiers in Microbiology.2021;[Epub] CrossRef
Streptococcus gordonii: Pathogenesis and Host Response to Its Cell Wall Components
Ok-Jin Park, Yeongkag Kwon, Chaeyeon Park, Yoon Ju So, Tae Hwan Park, Sungho Jeong, Jintaek Im, Cheol-Heui Yun, Seung Hyun Han
Microorganisms.2020; 8(12): 1852. CrossRef

Propionate, together with triple antibiotics, inhibits the growth of Enterococci: Soyoung Jeong , Yunjae Lee , Cheol-Heui Yun , Ok-Jin Park , Seung Hyun Han; J. Microbiol. 2019;57(11):1019-1024. Published online October 28, 2019; DOI: https://doi.org/10.1007/s12275-019-9434-7

46 View
0 Download
15 Web of Science
14 Crossref

Abstract

Enterococci are Gram-positive facultative anaerobic bacteria that colonize the oral cavity and gastrointestinal tract. Enterococcal infections, mainly caused by Enterococcus faecalis and Enterococcus faecium, include apical periodontitis, endocarditis, and bloodstream infections. Recently, vancomycinresistant Enterococci are considered major pathogens that are common but difficult to treat, especially in nosocomial settings. Moreover, E. faecalis is closely associated with recurrent endodontic infections and failed endodontic treatment. In this study, we investigated the effects of short-chain fatty acids (SCFAs), acetate, propionate, and butyrate, which are metabolites fermented by gut microbiota, on the growth of Enterococci. Enterococci were cultured in the presence or absence of acetate, propionate, or butyrate, and the optical density at 600 nm was measured to determine bacterial growth. The minimum inhibitory concentration/minimum bactericidal concentration test was conducted. Bacteria were treated with a SCFA, together with clinically used endodontic treatment methods such as triple antibiotics (metronidazole, minocycline, and ciprofloxacin) and chlorhexidine gluconate (CHX) to determine the effects of combination treatment. Of the SCFAs, propionate had a bacteriostatic effect, inhibiting the growth of E. faecalis in a dose-dependent manner and also that of clinical strains of E. faecalis isolated from dental plaques. Meanwhile, acetate and butyrate had minimal effects on E. faecalis growth. Moreover, propionate inhibited the growth of other Enterococci including E. faecium. In addition, combination treatment of propionate and triple antibiotics led to further growth inhibition, whereas no cooperative effect was observed at propionate plus CHX. These results indicate that propionate attenuates the growth of Enterococci, suggesting propionate as a potential agent to control Enterococcal infections, especially when combined with triple antibiotics.

Citations

Citations to this article as recorded by

Serotype-Dependent Inhibition of Streptococcus pneumoniae Growth by Short-Chain Fatty Acids
Suwon Lim, Dongwook Lee, Sungho Jeong, Jeong Woo Park, Jintaek Im, Bokeum Choi, Donghyun Gwak, Cheol-Heui Yun, Ho Seong Seo, Seung Hyun Han
Journal of Microbiology and Biotechnology.2024; 34(1): 47. CrossRef
Microbiota signatures associated with invasive Candida albicans infection in the gastrointestinal tract of immunodeficient mice
Jia-Ying Yan, Tsung-Han Lin, Yu-Tang Jong, Jun-Wei Hsueh, Sze-Hsien Wu, Hsiu-Jung Lo, Yee-Chun Chen, Chien-Hsiung Pan
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Diet-induced changes in the jejunal microbiota of developing broilers reduce the abundance of Enterococcus hirae and Enterococcus faecium
Paul B. Stege, Dirkjan Schokker, Frank Harders, Soumya K. Kar, Norbert Stockhofe, Vera Perricone, Johanna M. J. Rebel, Ingrid C. de Jong, Alex Bossers
BMC Genomics.2024;[Epub] CrossRef
Enterococcus faecium: evolution, adaptation, pathogenesis and emerging therapeutics
Yahan Wei, Dennise Palacios Araya, Kelli L. Palmer
Nature Reviews Microbiology.2024; 22(11): 705. CrossRef
Mechanisms of probiotic Bacillus against enteric bacterial infections
Jiajia Zhu, Yunsheng Chen, Kálmán Imre, Damla Arslan-Acaroz, Fatih Ramazan Istanbullugil, Yuwen Fang, Gaspar Ros, Kui Zhu, Ulas Acaroz
One Health Advances.2023;[Epub] CrossRef
Structural features and anticancer mechanisms of pectic polysaccharides: A review
Fangfang Yue, Jiaxin Xu, Sitan Zhang, Xinyu Hu, Xin Wang, Xin Lü
International Journal of Biological Macromolecules.2022; 209: 825. CrossRef
Dual role of microbiota-derived short-chain fatty acids on host and pathogen
Rasoul Mirzaei, Elahe Dehkhodaie, Behnaz Bouzari, Mandana Rahimi, Abolfazl Gholestani, Seyed Reza Hosseini-Fard, Hossein Keyvani, Ali Teimoori, Sajad Karampoor
Biomedicine & Pharmacotherapy.2022; 145: 112352. CrossRef
RNA-Seq-based transcriptome analysis of methicillin-resistant Staphylococcus aureus growth inhibition by propionate
Jintaek Im, Dongwook Lee, Ok-Jin Park, Sathishkumar Natarajan, Junhyung Park, Cheol-Heui Yun, Seung Hyun Han
Frontiers in Microbiology.2022;[Epub] CrossRef
Propionate Attenuates Growth of Oral Streptococci through Enhancing Methionine Biosynthesis
Taehwan Park, Jintaek Im, A Reum Kim, Dongwook Lee, Sungho Jeong, Cheol-Heui Yun, Seung Hyun Han
Journal of Microbiology and Biotechnology.2022; 32(10): 1234. CrossRef
Bacterial-Induced Blood Pressure Reduction: Mechanisms for the Treatment of Hypertension via the Gut
Tyler Alexander Cookson
Frontiers in Cardiovascular Medicine.2021;[Epub] CrossRef
Influence of operating conditions on the persistence of E. coli, enterococci, Clostridium perfringens and Clostridioides difficile in semi-continuous mesophilic anaerobic reactors
Derongs Lorine, Druilhe Céline, Le Maréchal Caroline, Barbut Frédéric, Heurtevent Lorette, Buffet Julie, Martin Laure, Ziebal Christine, Poezevara Typhaine, Rouxel Sandra, Houard Emmanuelle, Syed Zaidi Rabab, Couturier Jeanne, Pourcher Anne-Marie
Waste Management.2021; 134: 32. CrossRef
Short-Chain Fatty Acids as a Potential Treatment for Infections: a Closer Look at the Lungs
Marina Gomes Machado, Valentin Sencio, François Trottein, Andreas J. Bäumler
Infection and Immunity.2021;[Epub] CrossRef
The lung–gut axis during viral respiratory infections: the impact of gut dysbiosis on secondary disease outcomes
Valentin Sencio, Marina Gomes Machado, François Trottein
Mucosal Immunology.2021; 14(2): 296. CrossRef
Enhanced biofilm formation of Streptococcus gordonii with lipoprotein deficiency
Ok‐Jin Park, Solmin Jung, Taehwan Park, A Reum Kim, Dongwook Lee, Hyun Jung Ji, Ho Seong Seo, Cheol‐Heui Yun, Seung Hyun Han
Molecular Oral Microbiology.2020; 35(6): 271. CrossRef

Lipoteichoic acids of lactobacilli inhibit Enterococcus faecalis biofilm formation and disrupt the preformed biofilm: Solmin Jung , Ok-Jin Park , A Reum Kim , Ki Bum Ahn , Dongwook Lee , Kee-Yeon Kum , Cheol-Heui Yun , Seung Hyun Han; J. Microbiol. 2019;57(4):310-315. Published online January 22, 2019; DOI: https://doi.org/10.1007/s12275-019-8538-4

49 View
0 Download
49 Web of Science
47 Crossref

Abstract

Enterococcus faecalis, a Gram-positive bacterium commonly isolated in patients with refractory apical periodontitis, invades dentin tubules easily and forms biofilms. Bacteria in biofilms, which contribute to recurrent and/or chronic inflammatory diseases, are more resistant to antimicrobial agents than planktonic cells and easily avoid phagocytosis. Although Lactobacillus plantarum lipoteichoic acid (Lp.LTA) is associated with biofilm formation, the effect of Lp.LTA on biofilm formation by E. faecalis is not clearly understood. In this study, we investigated whether Lp.LTA inhibits E. faecalis biofilm formation. The degree of biofilm formation was determined by using crystal violet assay and LIVE/DEAD bacteria staining. The quantification of bacterial growth was determined by measuring the optical density at 600 nm with a spectrophotometer. Formation of biofilms on human dentin slices was observed under a scanning electron microscope. E. faecalis biofilm formation was reduced by Lp.LTA treatment in a dose-dependent manner. Lp.LTA inhibited biofilm development of E. faecalis at the early stage without affecting bacterial growth. LTA from other Lactobacillus species such as Lactobacillus acidophilus, Lactobacillus casei, or Lactobacillus rhamnosus GG also inhibited E. faecalis biofilm formation. In particular, among LTAs from various lactobacilli, Lp.LTA showed the highest inhibitory effect on biofilms formed by E. faecalis. Interestingly, LTAs from lactobacilli could remove the biofilm preformed by E. faecalis. These inhibitory effects were also observed on the surface of human dentin slices. In conclusion, Lactobacillus species LTA inhibits biofilm formation caused by E. faecalis and it could be used as an anti-biofilm agent for prevention or treatment against E. faecalis-associated diseases.

Citations

Citations to this article as recorded by

Inhibitory effect and mechanism of violacein on planktonic growth, spore germination, biofilm formation and toxin production of Bacillus cereus and its application in grass carp preservation
Xiangdi Lou, Qiang Zhou, Qiyue Jiang, Liping Lin, Wenwu Zhu, Xiaoyu Mei, Jianhua Xiong, Yanyan Gao
International Journal of Food Microbiology.2025; 426: 110917. CrossRef
The effects of Lactiplantibacillus plantarum 3-19 and Pediococcus pentosaceus 18-1 on preventing the accumulation of biogenic amines and promoting the production of volatile organic compounds during sour meat fermentation
Hao Shang, Ying Yue, Bingrui Guo, Chaofan Ji, Sufang Zhang, Liang Dong, Ilario Ferrocino, Luca Simone Cocolin, Xinping Lin
International Journal of Food Microbiology.2024; 421: 110806. CrossRef
Small cyclic dipeptide produced by Lactobacillus rhamnosus with anti-biofilm properties against Streptococcus mutans biofilm
Rashmi Niranjan, Sachin Patil, Amrita Dubey, Bimlesh Lochab, Richa Priyadarshini
Biofilm.2024; 8: 100237. CrossRef
Enterococcus Phage vB_EfaS_HEf13 as an Anti-Biofilm Agent Against Enterococcus faecalis
Dongwook Lee, Jintaek Im, A Reum Kim, Woohyung Jun, Cheol-Heui Yun, Seung Hyun Han
Journal of Microbiology.2024; 62(8): 683. CrossRef
Extracellular Proteins Isolated from L. acidophilus as an Osteomicrobiological Therapeutic Agent to Reduce Pathogenic Biofilm Formation, Regulate Chronic Inflammation, and Augment Bone Formation In Vitro
Abinaya Sindu Pugazhendhi, Anouska Seal, Megan Hughes, Udit Kumar, Elayaraja Kolanthai, Fei Wei, Jonathan D. Schwartzman, Melanie J. Coathup
Advanced Healthcare Materials.2024;[Epub] CrossRef
Strategies and mechanisms targeting Enterococcus faecalis biofilms associated with endodontic infections: a comprehensive review
Shipeng Yang, Xiuping Meng, Yuqi Zhen, Quzhen Baima, Yu Wang, Xinmiao Jiang, Zhibo Xu
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Lipoteichoic Acid from Lacticaseibacillus rhamnosus GG as a Novel Intracanal Medicament Targeting Enterococcus faecalis Biofilm Formation
Ji-Young Yoon, Somin Park, Dongwook Lee, Ok-Jin Park, WooCheol Lee, Seung Hyun Han
Journal of Microbiology.2024; 62(10): 897. CrossRef
Isolation, Identification and Antibacterial Characteristics of Lacticaseibacillus rhamnosus YT
Chengran Guan, Feng Li, Peng Yu, Xuan Chen, Yongqi Yin, Dawei Chen, Ruixia Gu, Chenchen Zhang, Bo Pang
Foods.2024; 13(17): 2706. CrossRef
Lactobacillus-derived components for inhibiting biofilm formation in the food industry
Jiaxun Li, Qiuxiang Zhang, Jianxin Zhao, Hao Zhang, Wei Chen
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef
Natural aggregation of Lactobacillus: Mechanisms and influencing factors
Shunhe Wang, Lulu Li, Leilei Yu, Fengwei Tian, Jianxin Zhao, Qixiao Zhai, Wei Chen
Food Bioscience.2024; 62: 105007. CrossRef
Hypericum perforatum L. (St. John's Wort) in broilers diet improve growth performance, intestinal microflora and immunity
Ziya İlhan, Muhittin Zengin, Oğuz Koray Bacaksız, Ergün Demir, İsmail Hakkı Ekin, Mehmet Ali Azman
Poultry Science.2024; 103(12): 104419. CrossRef
The role of Lactobacillus plantarum in oral health: a review of current studies
Xinyan Huang, Jianhang Bao, Mingzhen Yang, Yingying Li, Youwen Liu, Yuankun Zhai
Journal of Oral Microbiology.2024;[Epub] CrossRef
Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model
Shymaa Shaaban, Salma Genena, Alaaeldin Elraggal, Gamal M. Hamad, Marwa A. Meheissen, Sybel Moussa
BMC Oral Health.2023;[Epub] CrossRef
Antioxidant Effects and Probiotic Properties of Latilactobacillus sakei MS103 Isolated from Sweet Pickled Garlic
Heng Li, Changlin Chen, Yuanxin Li, Zhengqiang Li, Chen Li, Chang Luan
Foods.2023; 12(23): 4276. CrossRef
Postbiotics in Human Health: A Narrative Review
Linxi Ma, Huaijun Tu, Tingtao Chen
Nutrients.2023; 15(2): 291. CrossRef
Influence of dietary supplementation with new Lactobacillus strains on hematology, serum biochemistry, nutritional status, digestibility, enzyme activities, and immunity in dogs
Kamonporn Panja, Sathita Areerat, Pipatpong Chundang, Pornsucha Palaseweenun, Nattaphong Akrimajirachoote, Jaruwan Sitdhipo, Punnathorn Thaveethaptaikul, Pennapa Chonpathompikunlert, Kanidta Niwasabutra, Pongsathon Phapugrangkul, Attawit Kovitvadhi
Veterinary World.2023; : 834. CrossRef
Antibacterial and antibiofilm potential of Lacticaseibacillus rhamnosus YT and its cell-surface extract
Chengran Guan, Wenjuan Zhang, Jianbo Su, Feng Li, Dawei Chen, Xia Chen, Yujun Huang, Ruixia Gu, Chenchen Zhang
BMC Microbiology.2023;[Epub] CrossRef
Mechanisms of microbial interactions between probiotic microorganisms and Helicobacter pylori
Guzel Sh. Isaeva, R.A. Isaeva
Clinical Microbiology and Antimicrobial Chemotherapy.2023; 25(3): 225. CrossRef
Preparation of branched RG-I-rich pectin from red dragon fruit peel and the characterization of its probiotic properties
Meng-yuan Zhang, Jun Cai
Carbohydrate Polymers.2023; 299: 120144. CrossRef
The potential of paraprobiotics and postbiotics to modulate the immune system: A Review
Jeet P. Mehta, Sonal Ayakar, Rekha S. Singhal
Microbiological Research.2023; 275: 127449. CrossRef
Identification of soybean peptides and their effect on the growth and metabolism of Limosilactobacillus reuteri LR08
Chi Zhang, Shaoqi Xia, Yinxiao Zhang, Shuya Zhu, He Li, Xinqi Liu
Food Chemistry.2022; 369: 130923. CrossRef
Acquisition of Daptomycin Resistance by Enterococcus faecium Confers Collateral Sensitivity to Glycopeptides
Weiliang Zeng, Luozhu Feng, Changrui Qian, Tao Chen, Sipei Wang, Ying Zhang, Xiangkuo Zheng, Lingbo Wang, Shixing Liu, Tieli Zhou, Yao Sun
Frontiers in Microbiology.2022;[Epub] CrossRef
Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef
Subgingival microbiome in periodontitis and type 2 diabetes mellitus: an exploratory study using metagenomic sequencing
Xianjun Lu, Tingjun Liu, Jiani Zhou, Jia Liu, Zijian Yuan, Lihong Guo
Journal of Periodontal & Implant Science.2022; 52(4): 282. CrossRef
Hidden Agenda of Enterococcus Faecalis Lifestyle Transition: Planktonic To Sessile State
Parvathy Venkateswaran, Priya M Lakshmanan, Sudhiksha Muthukrishnan, Hema Bhagavathi, Sahana Vasudevan, Prasanna Neelakantan, Adline P Solomon
Future Microbiology.2022; 17(13): 1051. CrossRef
Probiotics in the treatment of Helicobacter pylori infection: reality and perspective
Guzel ISAEVA, Regina ISAEVA
Minerva Gastroenterology.2022;[Epub] CrossRef
Algal polysaccharide’s potential to combat respiratory infections caused by Klebsiella pneumoniae and Serratia marcescens biofilms
Jyoti Vishwakarma, Bhumika Waghela, Berness Falcao, Sirisha L. Vavilala
Applied Biochemistry and Biotechnology.2022; 194(2): 671. CrossRef
Phage therapy for refractory periapical periodontitis caused by Enterococcus faecalis in vitro and in vivo
Yingying Xiang, Chunlan Ma, Shuang Yin, Fei Song, Kunhao Qin, Yafang Ding, Xianghong Yang, Pengfei Song, Xiuling Ji, Yunlin Wei
Applied Microbiology and Biotechnology.2022; 106(5-6): 2121. CrossRef
Gram Positive Bacterial Lipoteichoic Acid Role in a Root Canal Infection – A Literature Review
Vinoo Subramaniam Ramachandran, Mensudar Rathakrishnan, Malathy Balaraman Ravindrran, Alargarsamy Venkatesh, Vidhya Shankari Shanmugasundaram, Karpagavinayagam Kumaraguru
Journal of Pure and Applied Microbiology.2021; 15(2): 534. CrossRef
In Vitro Effects of Lactobacillus plantarum LN66 and Antibiotics Used Alone or in Combination on Helicobacter pylori Mature Biofilm
Jianfu Ji, Hong Yang
Microorganisms.2021; 9(2): 424. CrossRef
Effects of Rose Bengal‐ and Methylene Blue‐Mediated Potassium Iodide‐Potentiated Photodynamic Therapy on Enterococcus faecalis: A Comparative Study
Ruijie Li, Lintian Yuan, Weiqian Jia, Man Qin, Yuguang Wang
Lasers in Surgery and Medicine.2021; 53(3): 400. CrossRef
HEMA-induced oxidative stress inhibits NF-κB nuclear translocation and TNF release from LTA- and LPS-stimulated immunocompetent cells
Helmut Schweikl, Margaritha Birke, Marialucia Gallorini, Christine Petzel, Carola Bolay, Claudia Waha, Karl-Anton Hiller, Wolfgang Buchalla
Dental Materials.2021; 37(1): 175. CrossRef
Lactobacillus rhamnosus and Lactobacillus casei Affect Various Stages of Gardnerella Species Biofilm Formation
Yuanhui He, Risu Na, Xiaoxi Niu, Bingbing Xiao, Huixia Yang
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Cell-Free Lactobacillus sp Supernatants Modulate Staphylococcus aureus Growth, Adhesion and Invasion to Human Osteoblast (HOB) Cells
Fatma Kalaycı Yüksek, Defne Gümüş, Gül İpek Gündoğan, Mine Anğ Küçüker
Current Microbiology.2021; 78(1): 125. CrossRef
Comparison of solithromycin with erythromycin in Enterococcus faecalis and Enterococcus faecium from China: antibacterial activity, clonality, resistance mechanism, and inhibition of biofilm formation
Yu Wang, Yanpeng Xiong, Zhanwen Wang, Jinxin Zheng, Guangjian Xu, Qiwen Deng, Zewen Wen, Zhijian Yu
The Journal of Antibiotics.2021; 74(2): 143. CrossRef
Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus
Jaejin Lee, Eunkyoung Shin, Jaeyeong Park, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(12): 1133. CrossRef
Lactobacillus plantarum Lipoteichoic Acids Possess Strain-Specific Regulatory Effects on the Biofilm Formation of Dental Pathogenic Bacteria
Dongwook Lee, Jintaek Im, Dong Hyun Park, Sungho Jeong, Miri Park, Seokmin Yoon, Jaewoong Park, Seung Hyun Han
Frontiers in Microbiology.2021;[Epub] CrossRef
Can Probiotics Emerge as Effective Therapeutic Agents in Apical Periodontitis? A Review
Gaurav Kumar, Sanjay Tewari, John Tagg, Michael Leonidas Chikindas, Igor V Popov, Santosh Kumar Tiwari
Probiotics and Antimicrobial Proteins.2021; 13(2): 299. CrossRef
Lactobacilli as Anti-biofilm Strategy in Oral Infectious Diseases: A Mini-Review
Barbara Giordani, Carola Parolin, Beatrice Vitali
Frontiers in Medical Technology.2021;[Epub] CrossRef
Interplay between Candida albicans and Lactic Acid Bacteria in the Gastrointestinal Tract: Impact on Colonization Resistance, Microbial Carriage, Opportunistic Infection, and Host Immunity
Karen D. Zeise, Robert J. Woods, Gary B. Huffnagle
Clinical Microbiology Reviews.2021;[Epub] CrossRef
Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods
Basavaprabhu H. Nataraj, Syed Azmal Ali, Pradip V. Behare, Hariom Yadav
Microbial Cell Factories.2020;[Epub] CrossRef
Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health
Wallace Jeng Yang Chee, Shu Yih Chew, Leslie Thian Lung Than
Microbial Cell Factories.2020;[Epub] CrossRef
Enhanced biofilm formation of Streptococcus gordonii with lipoprotein deficiency
Ok‐Jin Park, Solmin Jung, Taehwan Park, A Reum Kim, Dongwook Lee, Hyun Jung Ji, Ho Seong Seo, Cheol‐Heui Yun, Seung Hyun Han
Molecular Oral Microbiology.2020; 35(6): 271. CrossRef
Lactobacillus plantarum lipoteichoic acid disrupts mature Enterococcus faecalis biofilm
A. Reum Kim, Minji Kang, Yeon-Jee Yoo, Cheol-Heui Yun, Hiran Perinpanayagam, Kee-Yeon Kum, Seung Hyun Han
Journal of Microbiology.2020; 58(4): 314. CrossRef
Streptococcus gordonii: Pathogenesis and Host Response to Its Cell Wall Components
Ok-Jin Park, Yeongkag Kwon, Chaeyeon Park, Yoon Ju So, Tae Hwan Park, Sungho Jeong, Jintaek Im, Cheol-Heui Yun, Seung Hyun Han
Microorganisms.2020; 8(12): 1852. CrossRef
Using Probiotics as Supplementation for Helicobacter pylori Antibiotic Therapy
Jianfu Ji, Hong Yang
International Journal of Molecular Sciences.2020; 21(3): 1136. CrossRef
In-vitro effect of vaginal lactobacilli against group B Streptococcus
Giacomo Marziali, Claudio Foschi, Carola Parolin, Beatrice Vitali, Antonella Marangoni
Microbial Pathogenesis.2019; 136: 103692. CrossRef

Research Support, Non-U.S. Gov't

Food-Borne Enterococci and Their Resistance to Oxidative Stress: Barbora Vlková , Tomá&# , Gabriel Minárik , Lubomíra Tóthová , Hana Drahovská , Ján Tur&# , Peter Celec; J. Microbiol. 2011;49(4):657-662. Published online September 2, 2011; DOI: https://doi.org/10.1007/s12275-011-0296-x

26 View
0 Download
4 Scopus

Abstract: Enterococci are important food-borne pathogens that cause serious infections. Several virulence factors have been described including aggregation substance, gelatinase, cytolysin, and enterococcal surface protein. The ability to cause infections is mainly dependent on the response to oxidative stress due to the production of reactive oxygen species by immune cells. The aim of our study was to analyze the resistance of enterococcal strains from food to clinically relevant antiseptic agents with regard to the presence of selected virulence factors, and to uncover potential mechanisms of the antioxidative resistance. Eighty-two enterococcal isolates from Bryndza cheese were tested using in vitro growth assays to study the ability of these isolates to survive exposure to antiseptic agents – hydrogen peroxide, hypochlorite, and chlorhexidine. Virulence genotypes of the isolates were determined by PCR, and RT real time PCR was used for gene expression under oxidative stress. Resistance against antiseptic agents depends on the concentration of applied chemicals, on the time of exposure, but also on virulence factors of the enterococcal strains. Oxidative stress induces the expression of antioxidative enzymes and down-regulates the expression of prooxidative enzymes. These effects are dependent on the virulence genotype of the enterococcal strains. These findings are important for future research, especially concerning the role of enterococci in oral diseases.

Journal Article

In vitro Activity of Sodium Benzoate Against Clinically Relevant Enterococcus faecalis and Enterococcus faecium Isolates: Oguz Karabay , Esra Kocoglu , Nevin Ince , Tufan Sahan , Davut Ozdemir; J. Microbiol. 2006;44(1):129-131.; DOI: https://doi.org/2325 [pii]

28 View
0 Download

Abstract: The antimicrobial effects of sodium benzoate against Enterococcus faecalis and Enterococcus faecium were investigated. The MIC90 of sodium benzoate were 64 mg/L for E. faecalis and 32 mg/L for E. faecium, while the MBC90 were 128 mg/L and 64mg/L, respectively. Although further studies are required for clinical evidence, sodium benzoate seems to be effective against Enterococcus spp.

Published Erratums

Erratum] The transcription factor Cas5 suppresses hyphal morphogenesis during yeast-form growth in Candida albicans: Jong-Myeong Kim , Hye Yun Moon , Dong Wook Lee , Hyun Ah Kang , Jeong-Yoon Kim; J. Microbiol. 2021;59(11):1063-1063.; DOI: https://doi.org/10.1007/s12275-021-0326-2

38 View
0 Download

Erratum] Brevibacterium limosum sp. nov., Brevibacterium pigmenatum sp. nov., and Brevibacterium atlanticum sp. nov., three novel dye decolorizing actinobacteria isolated from ocean sediments: Shengxiang Pei , Siwen Niu , Fuquan Xie , Wenjing Wang , Shuang Zhang , Gaiyun Zhang; J. Microbiol. 2021;59(11):1064-1065.; DOI: https://doi.org/10.1007/s12275-021-0235-4

37 View
0 Download

First
Prev
Page of 1
Next
Last

Search