Journal Articles
- Lactobacillus gasseri BNR17 and Limosilactobacillus fermentum ABF21069 Ameliorate High Sucrose-Induced Obesity and Fatty Liver via Exopolysaccharide Production and β-oxidation
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Yu Mi Jo, Yoon Ji Son, Seul-Ah Kim, Gyu Min Lee, Chang Won Ahn, Han-Oh Park, Ji-Hyun Yun
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J. Microbiol. 2024;62(10):907-918. Published online October 17, 2024
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DOI: https://doi.org/10.1007/s12275-024-00173-6
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Abstract
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Obesity and metabolic dysfunction-associated fatty liver disease (MAFLD) are prevalent metabolic disorders with substantial global health implications that are often inadequately addressed by current treatments and may have side effects.
Probiotics have emerged as promising therapeutic agents owing to their beneficial effects on gut health and metabolism. This study investigated the synergistic effects of a probiotic combination of BNR17 and ABF21069 on obesity and MAFLD in C57BL/6 mice fed a high-sucrose diet. The probiotic combination significantly reduced body weight and fat accumulation compared with the high-sucrose diet. It also alleviated elevated serum leptin levels induced by a high-sucrose diet.
Histological analysis revealed a significant reduction in white adipose tissue and fatty liver in the mice treated with the probiotic combination. Furthermore, increased expression of genes related to β-oxidation, thermogenesis, and lipolysis suggested enhanced metabolic activity. The probiotic groups, particularly the BNR17 group, showed an increase in fecal exopolysaccharides, along with a tendency toward a lower expression of intestinal sugar transport genes, indicating reduced sugar absorption. Additionally, inflammatory markers in the liver tissue exhibited lower expression in the ABF21069 group than in the HSD group. Despite each strain in the combination group having distinct characteristics and functions, their combined effect demonstrated synergy in mitigating obesity and MAFLD, likely through the modulation of fecal exopolysaccharides content and improvement in lipid metabolism. These findings underscore the potential of probiotic supplementation as a promising assistant therapy for managing obesity and MAFLD and provide valuable insights into its therapeutic mechanisms in metabolic disorders.
- Effect of exopolysaccharides of Paenibacillus polymyxa rhizobacteria on physiological and morphological variables of wheat seedlings
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Irina V. Yegorenkova , Kristina V. Tregubova , Alexander I. Krasov , Nina V. Evseeva , Larisa Yu. Matora
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J. Microbiol. 2021;59(8):729-735. Published online July 24, 2021
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DOI: https://doi.org/10.1007/s12275-021-0623-9
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Abstract
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Paenibacillus polymyxa is a promising plant-growth-promoting
rhizobacterium that associates with a wide range of host
plants, including agronomically important ones. Inoculation
of wheat seedlings with P. polymyxa strains CCM 1465 and
92 was found to increase the mitotic index of the root cells
1.2- and 1.6-fold, respectively. Treatment of seedlings with
the exopolysaccharides (EPSs) of these strains increased the
mitotic index 1.9-fold (P. polymyxa CCM 1465) and 2.8-fold
(P. polymyxa 92). These increases indicate activation of cell
division in the root meristems. Analysis of the morphometric
variables of the seedlings showed that P. polymyxa CCM
1465, P. polymyxa 92, and their EPSs promoted wheat growth,
increasing root and shoot length up to 22% and root and
shoot dry weight up to 28%, as compared with the control.
In addition, both strains were found to intensely colonize the
seedling root surface. Thus, P. polymyxa EPSs are active metabolites
that, along with whole cells, are responsible for the
contact interactions of the bacteria with wheat roots and are
implicated in the induction of plant responses to these interactions.
The strains used in this work are of interest for
further study to broaden the existing understanding of the
mechanisms of plant–bacterial interactions and to develop
effective biofertilizers for agricultural purposes.
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Citations
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- Optimization of the Production Parameters of a Novel Exopolysaccharide Chrysosporine from Endophytic Chrysosporium sp. KTL2, and Evaluation of its Antioxidative and Prebiotic Potentialities
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Frontiers in Plant Science.2024;[Epub] CrossRef - Screening of Endophytic Antagonistic Bacteria in Wheat and Evaluation of Biocontrol Potential against Wheat Stripe Rust
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International Journal of Molecular Sciences.2024; 25(13): 6877. CrossRef - Exopolysaccharides of Paenibacillus polymyxa: A review
Xuan-Ya Huang, Xin-Pei Ye, Yan-Yu Hu, Zhen-Xing Tang, Tian Zhang, Hai Zhou, Ting Zhou, Xue-Lian Bai, Er-Xu Pi, Bing-Hua Xie, Lu-E Shi
International Journal of Biological Macromolecules.2024; 261: 129663. CrossRef - Bacterial Inoculation and Co-Inoculation Improves Durum Wheat Productivity in Alkaline Calcareous Soils
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Review
- [MINIREVIEW] Interdependence between iron acquisition and biofilm formation in Pseudomonas aeruginosa
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Donghoon Kang , Natalia V. Kirienko
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J. Microbiol. 2018;56(7):449-457. Published online June 14, 2018
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DOI: https://doi.org/10.1007/s12275-018-8114-3
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47
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Abstract
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Bacterial biofilms remain a persistent threat to human healthcare
due to their role in the development of antimicrobial
resistance. To combat multi-drug resistant pathogens, it is
crucial to enhance our understanding of not only the regulation
of biofilm formation, but also its contribution to bacterial
virulence. Iron acquisition lies at the crux of these two
subjects. In this review, we discuss the role of iron acquisition
in biofilm formation and how hosts impede this mechanism
to defend against pathogens. We also discuss recent findings
that suggest that biofilm formation can also have the reciprocal
effect, influencing siderophore production and iron
sequestration.
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Citations
Citations to this article as recorded by

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Journal Article
- Potential use of lactic acid bacteria Leuconostoc mesenteroides as a probiotic for the removal of Pb(II) toxicity
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Young-Joo Yi , Jeong-Muk Lim , Suna Gu , Wan-Kyu Lee , Eunyoung Oh , Sang-Myeong Lee , Byung-Taek Oh
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J. Microbiol. 2017;55(4):296-303. Published online March 31, 2017
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DOI: https://doi.org/10.1007/s12275-017-6642-x
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Abstract
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It has been demonstrated that certain lactic acid bacteria (LAB) can sequester metal ions by binding them to their surfaces. In the present study, lead (Pb)-resistant LAB were isolated from kimchi, a Korean fermented food. A total of 96 different LAB strains were isolated, and 52 strains showed lead resistance. Among them, an LAB strain-96 (L-96) iden-tified as Leuconostoc mesenteroides showed remarkable Pb resistance and removal capacity. The maximum adsorption capacity of this strain calculated using the Langmuir isotherm was 60.6 mg Pb/g. In an in vivo experiment, young male mice were provided with water (A), Pb-water (B), or Pb-water+ L-96 (C) during puberty. Lower glutamate oxaloacetate trans-aminase (GOT) and glutamate pyruvate transaminase (GPT) levels in Pb-exposed male mice that received strain L-96 as a probiotic were suggestive of reduced hepatotoxicity. More-over, feces from mice treated with L-96 contained more Pb than feces from untreated mice. Increased Pb elimination likely reduced internal accumulation, and this hypothesis was supported by significantly lower Pb concentrations in kid-neys and testes of the mice treated with strain L-96. The mo-tility and ATP content of epididymal spermatozoa were par-tially restored if strain L-96 was administered. In conclusion, isolated L-96 LAB had lead-biosorption activity and effi-ciently detoxified lead-poisoned male mice, resulting in re-covering male reproductive function. These results suggest the potential use of LAB as a probiotic to protect humans from the adverse effects of Pb exposure.
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Citations
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Research Support, Non-U.S. Gov't
- Enhanced Production of Exopolysaccharides by Fed-batch Culture of Ganoderma resinaceum DG-6556
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DOI: https://doi.org/2360 [pii]
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Abstract
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The objectives of this study were to optimize submerged culture conditions of a new fungal isolate, Ganorderma resinaceum, and to enhance the production of bioactive mycelial biomass and exopolysaccharides (EPS) by fed-batch culture. The maximum mycelial growth and EPS production in batch culture were achieved in a medium containing 10 g/l glucose, 8 g/l soy peptone, and 5 mM MnCl2 at an initial pH 6.0 and temperature 31°C. After optimization of culture medium and environmental conditions in batch cultures, a fed-batch culture strategy was employed to enhance production of mycelial biomass and EPS. Five different EPS with molecular weights ranging from 53,000 to 5,257,000 g/mole were obtained from either top or bottom fractions of ethanol precipitate of culture filtrate. A fed-batch culture of G. resinaceum led to enhanced production of both mycelial biomass
and EPS. The maximum concentrations of mycelial biomass (42.2 g/l) and EPS (4.6 g/l)
were obtained when 50 g/l of glucose was fed at day 6 into an initial 10 g/l of glucose
medium. It may be worth attempting with other mushroom fermentation processes for
enhanced production of mushroom polysaccharides, particularly those with industrial
potential.