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- Cloning and Functional Analysis of the Gβ Gene Mgb1 and the Gγ Gene Mgg1 in Monascus ruber
- Li Li , Lu He , Yong Lai , Yanchun Shao , Fusheng Chen
- J. Microbiol. 2014;52(1):35-43. Published online January 4, 2014
- DOI: https://doi.org/10.1007/s12275-014-3072-x
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- 28 Crossref
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Abstract
-
The ascomycetous fungus Monascus ruber is one of the most
well-known species widely used to produce Monascus-fermentation
products for natural food colorants and medicine.
Our previous research on the Gα subunit Mga1 and the regulator
of G protein signaling MrflbA indicated that heterotrimeric
G protein signaling pathways were involved in aspects
of growth, sporulation and secondary metabolite production
in M. ruber. To better understand the G protein signaling
pathways in this fungus, a Gβ subunit gene (Mgb1)
and a Gγ subunit gene (Mgg1) were cloned and investigated
in the current study. The predicted Mgb1 protein consisted
of 353 amino acids and Mgg1 consisted of 94 amino acids,
sharing marked similarity with Aspergillus Gβ and Gγ subunits,
respectively. Targeted deletion (Δ) of Mgb1 or Mgg1
result
ed in phenotypic alterations similar to those resulting from ΔMga1, i.e., restricted vegetative growth, lowered asexual sporulation, impaired cleistothecial formation, and enhanced citrinin and pigment production. Moreover, deletion of Mgg1 suppressed the defects in asexual development and in biosynthesis of citrinin and pigment caused by the absence of MrflbA function. These results provide evidence that Mgb1 and Mgg1 form a functional Gβγ dimer and the dimer interacts with Mga1 to mediate signaling pathways, which are negatively controlled by MrflbA, for growth, reproduction and citrinin and pigment biosynthesis in M. ruber. -
Citations
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