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- REVIEW] All about that fat: Lipid modification of proteins in Cryptococcus neoformans
- Felipe H. Santiago-Tirado , Tamara L. Doering
- J. Microbiol. 2016;54(3):212-222. Published online February 27, 2016
- DOI: https://doi.org/10.1007/s12275-016-5626-6
- 55 View
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- 8 Crossref
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Abstract
- Lipid modification of proteins is a widespread, essential process whereby fatty acids, cholesterol, isoprenoids, phospholipids, or glycosylphospholipids are attached to polypeptides. These hydrophobic groups may affect protein structure, function, localization, and/or stability; as a consequence such modifications play critical regulatory roles in cellular systems. Recent advances in chemical biology and proteomics have allowed the profiling of modified proteins, enabling dissection of the functional consequences of lipid addition. The enzymes that mediate lipid modification are specific for both the lipid and protein substrates, and are conserved from fungi to humans. In this article we review these enzymes, their substrates, and the processes involved in eukaryotic lipid modification of proteins. We further focus on its occurrence in the fungal pathogen Cryptococcus neoformans, highlighting unique features that are both relevant for the biology of the organism and potentially important in the search for new therapies.
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Citations
Citations to this article as recorded by
- Acylation of non‐specific phospholipase C4 determines its function in plant response to phosphate deficiency
Bao Yang, Ke Zhang, Xiong Jin, Jiayu Yan, Shaoping Lu, Qingwen Shen, Lei Guo, Yueyun Hong, Xuemin Wang, Liang Guo
The Plant Journal.2021; 106(6): 1647. CrossRef - Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases
Yuan Sui, Michael Wisniewski, Samir Droby, Edoardo Piombo, Xuehong Wu, Junyang Yue
Frontiers in Microbiology.2020;[Epub] CrossRef - Peeling the onion: the outer layers of Cryptococcus neoformans
Daniel P Agustinho, Liza C Miller, Lucy X Li, Tamara L Doering
Memórias do Instituto Oswaldo Cruz.2018;[Epub] CrossRef - The molecular era of protein S-acylation: spotlight on structure, mechanisms, and dynamics
María-Eugenia Zaballa, F. Gisou van der Goot
Critical Reviews in Biochemistry and Molecular Biology.2018; 53(4): 420. CrossRef - Glucosamine stimulates pheromone-independent dimorphic transition in Cryptococcus neoformans by promoting Crz1 nuclear translocation
Xinping Xu, Jianfeng Lin, Youbao Zhao, Elyssa Kirkman, Yee-Seul So, Yong-Sun Bahn, Xiaorong Lin, Aaron P. Mitchell
PLOS Genetics.2017; 13(9): e1006982. CrossRef - Alpha-helicoidal HEAT-like Repeat Proteins (αRep) Selected as Interactors of HIV-1 Nucleocapsid Negatively Interfere with Viral Genome Packaging and Virus Maturation
Sudarat Hadpech, Sawitree Nangola, Koollawat Chupradit, Kanda Fanhchaksai, Wilhelm Furnon, Agathe Urvoas, Marie Valerio-Lepiniec, Philippe Minard, Pierre Boulanger, Saw-See Hong, Chatchai Tayapiwatana
Scientific Reports.2017;[Epub] CrossRef - Human fungal pathogens: Why should we learn?
Jeong-Yoon Kim
Journal of Microbiology.2016; 54(3): 145. CrossRef - Interactions between Melanin Enzymes and Their Atypical Recruitment to the Secretory Pathway by Palmitoylation
Srijana Upadhyay, Xinping Xu, Xiaorong Lin, J. Andrew Alspaugh
mBio.2016;[Epub] CrossRef
- Acylation of non‐specific phospholipase C4 determines its function in plant response to phosphate deficiency
Research Support, Non-U.S. Gov't
- Cell-Surface Expression of Aspergillus saitoi-Derived Functional α-1,2-Mannosidase on Yarrowia lipolytica for Glycan Remodeling
- Hye Yun Moon , Trinh Luu Van , Seon Ah Cheon , Jinho Choo , Jeong-Yoon Kim , Hyun Ah Kang
- J. Microbiol. 2013;51(4):506-514. Published online August 30, 2013
- DOI: https://doi.org/10.1007/s12275-013-3344-x
- 40 View
- 0 Download
- 13 Crossref
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Abstract
- Expression of proteins on the surface of yeast has a wide range of applications, such as development of live vaccines, screening of antibody libraries, and use as whole-cell biocatalysts. The hemiascomycetes yeast Yarrowia lipolytica has been raised as a potential host for heterologous expression of recombinant proteins. In this study, we report the expression of Aspergillus saitoi α-1,2-mannosidase, encoded by the msdS gene, on the cell surface of Y. lipolytica. As the first step to achieve the secretory expression of msdS protein, four different signal sequences-derived from the endogenous Y. lipolytica Lip2 and Xpr2 prepro regions and the heterologous A. niger α-amylase and rice α-amylase signal sequences-were analyzed for their secretion efficiency. It was shown that the YlLip2 prepro sequence was most efficient in directing the secretory expression of msdS in fully N-glycosylated forms. The surface display of msdS was subsequently directed by fusing GPI anchoring motifs derived from Y. lipolytica cell wall proteins, YlCwp1p and YlYwp1p, respectively, to the C-terminus of the Lip2 prepro-msdS protein. The expression of actively functional msdS protein on the cell surface was confirmed by western blot, flow cytometry analysis, along with the α-1,2-mannosidase activity assay using intact Y. lipolytica cells as the enzyme source. Furthermore, the glycoengineered Y. lipolytica Δoch1Δmpo1 strains displaying α-1,2-mannosidase were able to convert Man8GlcNAc2 to Man5GlcNAc2 efficiently on their cell-wall mannoproteins, demonstrating its potential used for glycoengineering in vitro or in vivo.
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Citations
Citations to this article as recorded by- Engineering novel Yarrowia lipolytica whole-cell biocatalysts by cell surface display of the native Lip2 lipase for biodiesel production
Maria Orfanidou, Eleftheria Panagiotidou, Antonios M. Makris, Eleni Theodosiou
Biotechnology for the Environment.2025;[Epub] CrossRef - Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications
Karla V. Teymennet-Ramírez, Fernando Martínez-Morales, María R. Trejo-Hernández
Frontiers in Bioengineering and Biotechnology.2022;[Epub] CrossRef - Dietary intake of GDF11 delays the onset of several biomarkers of aging in male mice through anti-oxidant system via Smad2/3 pathway
Lili Song, Fei Wu, Congjun Li, Shicui Zhang
Biogerontology.2022; 23(3): 341. CrossRef - Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin
Ewelina Celińska, Jean-Marc Nicaud, Wojciech Białas
Applied Microbiology and Biotechnology.2021; 105(3): 975. CrossRef - Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement
Catherine Madzak
Journal of Fungi.2021; 7(7): 548. CrossRef - Engineering Yarrowia lipolytica for Use in Biotechnological Applications: A Review of Major Achievements and Recent Innovations
Catherine Madzak
Molecular Biotechnology.2018; 60(8): 621. CrossRef - Synthetic biology tools for engineering Yarrowia lipolytica
M. Larroude, T. Rossignol, J.-M. Nicaud, R. Ledesma-Amaro
Biotechnology Advances.2018; 36(8): 2150. CrossRef - Development of recombinant Yarrowia lipolytica producing virus-like particles of a fish nervous necrosis virus
Van-Trinh Luu, Hye Yun Moon, Jee Youn Hwang, Bo-Kyu Kang, Hyun Ah Kang
Journal of Microbiology.2017; 55(8): 655. CrossRef - Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica
Rémi Dulermo, François Brunel, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jérémy Vion, Marion Trassaert, Stéphane Thomas, Jean-Marc Nicaud, Christophe Leplat
Microbial Cell Factories.2017;[Epub] CrossRef -
Yarrowia lipolytica: recent achievements in heterologous protein expression and pathway engineering
Catherine Madzak
Applied Microbiology and Biotechnology.2015; 99(11): 4559. CrossRef - Biotechnological applications of Yarrowia lipolytica: Past, present and future
Hu-Hu Liu, Xiao-Jun Ji, He Huang
Biotechnology Advances.2015; 33(8): 1522. CrossRef - Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica
Jeong-Nam Park, Chang Pyo Han, Dong-Jik Lee, Seon Ah Cheon, Hyun Ah Kang
Journal of Microbiology.2014; 52(4): 284. CrossRef - Characterization of putative glycosylphosphatidylinositol-anchoring motifs for surface display in the methylotrophic yeast Hansenula polymorpha
Seon Ah Cheon, Jinhee Jung, Jin Ho Choo, Doo-Byoung Oh, Hyun Ah Kang
Biotechnology Letters.2014; 36(10): 2085. CrossRef
- Engineering novel Yarrowia lipolytica whole-cell biocatalysts by cell surface display of the native Lip2 lipase for biodiesel production
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