Journal of Microbiology

Journal Articles

Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus: Jaejin Lee , Eunkyoung Shin , Jaeyeong Park , Minho Lee , Kangseok Lee; J. Microbiol. 2021;59(12):1133-1141. Published online November 9, 2021; DOI: https://doi.org/10.1007/s12275-021-1518-5

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RraA, a protein regulator of RNase E activity, plays a unique role in modulating the mRNA abundance in Escherichia coli. The marine pathogenic bacterium Vibrio vulnificus also possesses homologs of RNase E (VvRNase E) and RraA (VvRraA1 and VvRraA2). However, their physiological roles have not yet been investigated. In this study, we demonstrated that VvRraA1 expression levels affect the pathogenicity of V. vulnificus. Compared to the wild-type strain, the VvrraA1-deleted strain (ΔVvrraA1) showed decreased motility, invasiveness, biofilm formation ability as well as virulence in mice; these phenotypic changes of ΔVvrraA1 were restored by the exogenous expression of VvrraA1. Transcriptomic analysis indicated that VvRraA1 expression levels affect the abundance of a large number of mRNA species. Among them, the halflives of mRNA species encoding virulence factors (e.g., smcR and htpG) that have been previously shown to affect VvrraA1 expression-dependent phenotypes were positively correlated with VvrraA1 expression levels. These findings suggest that VvRraA1 modulates the pathogenicity of V. vulnificus by regulating the abundance of a subset of mRNA species.

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Identification of the global regulatory roles of RraA via the integrative transcriptome and proteome in Vibrio alginolyticus
Huizhen Chen, Qian Gao, Bing Liu, Ying Zhang, Jianxiang Fang, Songbiao Wang, Youqi Chen, Chang Chen, Nicolas E. Buchler
mSphere.2024;[Epub] CrossRef
Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant
Seungmok Han, Ji-Won Byun, Minho Lee
Journal of Microbiology.2024; 62(1): 33. CrossRef
Eco-Evolutionary Drivers of Vibrio parahaemolyticus Sequence Type 3 Expansion: Retrospective Machine Learning Approach
Amy Marie Campbell, Chris Hauton, Ronny van Aerle, Jaime Martinez-Urtaza
JMIR Bioinformatics and Biotechnology.2024; 5: e62747. CrossRef
Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I
Dayeong Bae, Hana Hyeon, Eunkyoung Shin, Ji-Hyun Yeom, Kangseok Lee
Journal of Microbiology.2023; 61(2): 211. CrossRef
Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef

Leifsonia flava sp. nov., a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora: Ying Cai , Wen-Zhen Tao , Yu-Jun Ma , Juan Cheng , Meng-Yue Zhang , Yi-Xuan Zhang; J. Microbiol. 2018;56(8):549-555. Published online July 25, 2018; DOI: https://doi.org/10.1007/s12275-018-8061-z

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Abstract

SYP-B2174T is a yellow-pigmented, Gram-positive, non-motile, and rod-shaped actinobacterium isolated from the rhizospheric soil of Aquilegia viridiflora Pall. collected from the Xinjiang uygur autonomous region of China. The strain’s growth temperature ranges from 1 to 35°C, with an optimal growth being observed at 28°C. Growth occurs from 0 to 5% NaCl and at pH 6–8, with optimal growth being observed in 1% NaCl at pH 7. Comparative 16S rRNA gene sequencebased phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis JCM 17021T and Leifsonia psychrotolerans DSM 22824T with similarities of 97.8 and 97.6%, respectively. The DNA-DNA hybridization values of the strain SYP-B2174T to its closest phylogenetic neighbors were significantly lower than 35.7%. The strain was identified as a novel species of the genus Leifsonia judging by the coryneform morphology, peptidoglycans based upon 2,4-diaminobutyric acid, principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, major menaquinone MK-11, predominant fatty acids of anteiso-C15:0, anteiso-C17:0, and iso-C16:0, and a DNA G + C base composition of 68.7 mol%, for which the name Leifsonia flava sp. nov. is proposed. The type strain is SYP-B2174T (= CGMCC 1.15856T = DSM 105144T = KCTC 39963T).

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Enhancement of the germination and growth of Panicum miliaceum and Brassica juncea in Cd- and Zn-contaminated soil inoculated with heavy-metal-tolerant Leifsonia sp. ZP3
Ian Cho, Soo Yeon Lee, Kyung-Suk Cho
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef
Plant Growth-Promoting and Tequila Vinasse-Resistant Bacterial Strains
Alberto J. Valencia-Botín, Ismael F. Chávez-Díaz, Florentina Zurita-Martínez, Allan Tejeda-Ortega, Lily X. Zelaya-Molina
Microbiology Research.2024; 15(3): 1144. CrossRef
Phylogenomics, phenotypic, and functional traits of five novel (Earth-derived) bacterial species isolated from the International Space Station and their prevalence in metagenomes
Anna C. Simpson, Pratyay Sengupta, Flora Zhang, Asif Hameed, Ceth W. Parker, Nitin K. Singh, Georgios Miliotis, Punchappady D. Rekha, Karthik Raman, Christopher E. Mason, Kasthuri Venkateswaran
Scientific Reports.2023;[Epub] CrossRef
Diversity of Culturable Bacteria Isolated from Highland Barley Cultivation Soil in Qamdo, Tibet Autonomous Region
HU PAN, JIE ZHOU, ZHUOMA DAWA, YANNA DAI, YIFAN ZHANG, HUI YANG, CHONG WANG, HUHU LIU, HUI ZHOU, XIANGYANG LU, YUN TIAN
Polish Journal of Microbiology.2021; 70(1): 87. CrossRef
Unlocking the potential of plant growth-promoting rhizobacteria on soil health and the sustainability of agricultural systems
Zobia Khatoon, Suiliang Huang, Mazhar Rafique, Ali Fakhar, Muhammad Aqeel Kamran, Gustavo Santoyo
Journal of Environmental Management.2020; 273: 111118. CrossRef
Notification of changes in taxonomic opinion previously published outside the IJSEM
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology.2019; 69(1): 13. CrossRef

Research Support, Non-U.S. Gov'ts

Paenibacillus insulae sp. nov., isolated from soil: Sung-Jun Cho , Sung-Heun Cho , Tae-Su Kim , Suhk-Hwan Park , Seung-Bum Kim , Geon-Hyoung Lee; J. Microbiol. 2015;53(9):588-591. Published online August 27, 2015; DOI: https://doi.org/10.1007/s12275-015-4610-x

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Abstract

A Gram-stain-positive, motile, endospore-forming, and strictly aerobic rod-shaped bacterium designated DS80T was isolated from an island soil. The strain DS80T grew at temperatures between 15 and 40°C (optimum = 30°C) and at pH values ranging from 5.0 to 9.0 (optimum = 7.0). The phylogenetic analysis based on the comparisons of the 16S rRNA gene sequences showed that the isolate was affiliated to the genus Paenibacillus and was mostly related to Paenibacillus assamensis GPTSA11T (with the sequence similarity of 96.33%) and Paenibacillus urinalis 5402403T(95.48%). The G+C content of the genomic DNA was 44.0 mol% and the major fatty acids were anteiso-C15:0, iso-C15:0, iso-C16:0, and C16:1 ω11c. Strain DS80T contained MK-7 as the major menaquinone, and phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol as the major polar lipids. The peptidoglycan contained a major amount of meso-diaminopimelic acid. The chemotaxonomic profile of strain DS80T was consistent with that of Paenibacillus. However, the phenotypic properties clearly separated the strain from other species of the genus. Accordingly, a new species, Paenibacillus insulae sp. nov., is proposed (type strain =DS80T =JCM 17278T =KCTC 13833T).

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Enhancement of stability and activity of RSD amylase from Paenibacillus lactis OPSA3 for biotechnological applications by covalent immobilization on green silver nanoparticles
Emmanuel Tobechukwu Ugwuoji, Ifeanyi S. Eze, Tochukwu Nwamaka T. Nwagu, Lewis Iheanacho Ezeogu
International Journal of Biological Macromolecules.2024; 279: 135132. CrossRef
Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology
Emmanuel Tobechukwu Ugwuoji, Tochukwu Nwamaka T. Nwagu, Lewis Iheanacho Ezeogu
Biotechnology Reports.2023; 39: e00808. CrossRef
Characterization of cellulose-degrading microbiota from the eastern subterranean termite and soil
Xianfa Xie, Alonzo B. Anderson, Latoya J. Wran, Myrna G. Serrano, Gregory A. Buck
F1000Research.2021; 6: 2082. CrossRef
Paenibacillus oryzisoli sp. nov., isolated from the rhizosphere of rice
Jun Zhang, Xiao-Tong Ma, Jun-Sheng Gao, Cai-Wen Zhang, Juan-Juan Zhao, Rui-Jie Zhang, Li-An Ma, Xiao-Xia Zhang
Antonie van Leeuwenhoek.2017; 110(1): 69. CrossRef
Characterization of cellulose-degrading microbiota from the eastern subterranean termite and soil
Xianfa Xie, Alonzo B. Anderson, Latoya J. Wran, Myrna G. Serrano, Gregory A. Buck
F1000Research.2017; 6: 2082. CrossRef

Paenibacillus telluris sp. nov., a Novel Phosphate-Solubilizing Bacterium Isolated from Soil: Jae-Chan Lee , Chang-Jin Kim , Ki-Hong Yoon; J. Microbiol. 2011;49(4):617-621. Published online September 2, 2011; DOI: https://doi.org/10.1007/s12275-011-0471-0

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Abstract: A phosphate-solubilizing bacterial strain designated PS38T was isolated from farm soil. The isolate was a Gram-positive, motile, endospore-forming, rod-shaped bacterium. It grew optimally at 37oC and pH 7.5. The predominant cellular fatty acids were anteiso-C15:0, anteiso-C17:0, and iso-C16:0. The DNA G+C content was 49.5 mol% and the predominant menaquinone was MK-7. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain PS38T belonged to the genus Paenibacillus and was most closely related to Paenibacillus chibensis JCM 9905T, P. barengoltzii SAFN-016T, P. timonensis 2301032T, and P. motobuensis MC10T with 96.3%, 96.0%, 95.9%, and 95.5% 16S rRNA gene sequence similarity, respectively. On the basis of morphological, chemotaxonomic, physiological, and phylogenetic properties, strain PS38T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus telluris sp. nov. is proposed. The type strain is PS38T (=KCTC 13946T =CGMCC 1.10695T).

Journal Article

Epidemiological Features and Resistance Pattern in Uropathogens Isolated from Chronic Bacterial Prostatitis: Tommaso Cai , Sandra Mazzoli , Francesca Meacci , Vieri Boddi , Nicola Mondaini , Gianni Malossini , Riccardo Bartoletti; J. Microbiol. 2011;49(3):448-454. Published online June 30, 2011; DOI: https://doi.org/10.1007/s12275-011-0391-z

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Abstract: Chronic bacterial prostatitis (CBP) is, usually, caused by uropathogens, especially gram-negative bacilli, although infection is sometimes due to Gram-positive and atypical microorganisms. A recent increasing in prevalence of Gram-positive strains has been reported. The aim of this study was to explore the epidemiological features and resistance rates in uropathogens isolated from CBP outpatients in last 10 years. All consecutive outpatients with demonstrated CBP attending a single Sexually Transmitted Disease centre from January 1997 and December 2008, were enrolled and underwent microbiological cultures in first void early morning urine, midstream urine, expressed prostatic secretion, and post prostate massage urine. Prevalence of different bacterial strains was stratified in four different periods: 1997-1999, 2000-2002, 2003-2005, 2006-2008. Any changes observed in epidemiological features and resistance rates in uropathogens over the whole study period have been analyzed. The present study has been planned, thus, as in vitro study. From 6,221 patients, 4,601 Gram-positive and 1,620 Gram-negative bacterial strains have been isolated. Enterococcus faecalis and Escherichia coli strains are the first and second frequent pathogens found, respectively. Significant differences between E. faecalis prevalence in the 1997-1999 and 2006-2008 periods were found. E. coli showed a significant difference between prevalence in 1997-1999 and 2006-2008 periods. Gram-positive organisms showed a decreasing of susceptibility to ciprofloxacin as well as Gram-negative strains, while a good susceptibility to the levofloxacin was evidenced. E. faecalis prevalence seemed to be raised in 2006-2008 periods. Moreover, a decreasing of activity of ciprofloxacin and a good activity profile of levofloxacin have been reported.

Research Support, Non-U.S. Gov'ts

Oceanobacillus manasiensis sp. nov., a Moderately Halophilic Bacterium Isolated from the Salt Lakes of Xinjiang, China: Lei Wang , Wen-Yan Liu , Zhi-Jing Gu , San-Feng Chen , Su-Sheng Yang; J. Microbiol. 2010;48(3):312-317. Published online June 23, 2010; DOI: https://doi.org/10.1007/s12275-010-0135-5

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Abstract: Three Gram reaction positive, rod-shaped, moderately motile halophilic bacterial strains, designated YD3-56T, YD16, and YH29, were isolated from the sediments of Manasi and Aiding salt lakes in the Xinjiang region of China, respectively. The strains grew optimally at 30-37°C, pH 8-11, in the presence of 5-10% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains were closely related to members of the genus Oceanobacillus, exhibiting 99.1-99.2% similarity to O. kapialis KCTC 13177T, 99.2-99.3% to O. picturae KCTC 3821T, and 94.2-96% sequence similarity to other described Oceanobacillus species. SDS-PAGE of whole cell proteins preparations demonstrated that the strains exhibited high similarity to each other, but distinguished from O. kapialis KCTC 13177T and O. picturae KCTC 3821T (75%). DNA-DNA hybridization revealed that the similarity between the representative strain YD3-56T and O. kapialis KCTC 13177T was 35.3%, and the similarity between YD3-56T and O. picturae KCTC 3821T was 22.3%. Chemotaxonomic analysis of the strains showed menaquinone-7 was the predominant respiratory quinine. Major cellular fatty acids were anteiso-C15:0 and anteiso-C17:0. The polar lipid pattern for strain YD3-56T predominantly contained phosphatidylcholine, and trace to moderate amounts of phosphatidyl ethanolamine and hydroxy-phosphatidyl ethanolamine. The diamino acid in murein was meso-diaminopimelic acid. The DNA G+C content of the strains was 39.7-40.1 mol%. On the basis of these results, the three strains should be classified as a novel species of the genus Oceanobacillus, for which the name Oceanobacillus manasiensis sp. nov. has been proposed, with the type strain as YD3-56T (=CGMCC 1.9105T =NBRC 105903T).

Virgibacillus xinjiangensis sp. nov., Isolated from a Salt Lake of Xin-jiang Province in China: Che Ok Jeon , Jeong Myeong Kim , Dong-Jin Park , Li-Hua Xu , Cheng-Lin Jiang , Chang-Jin Kim; J. Microbiol. 2009;47(6):705-709. Published online February 4, 2010; DOI: https://doi.org/10.1007/s12275-009-0107-9

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Abstract: A strictly aerobic Gram-positive, moderately halophilic spore forming bacterium, designated strain SL6-1T, was isolated from a salt lake in Xin-jiang province, China. Growth of strain SL6-1T was observed at NaCl concentrations of 0~20% (w/v) (the optimum being 5~7%, w/v). The peptidoglycan type of strain SL6-1T was A1γ-meso-diaminopimelic acid and its major cellular fatty acids were iso-C14:0 and iso-C16:0 and anteiso-C15:0. The major respiratory isoprenoid quinone was MK-7 and the G+C content of the genomic DNA was 44.5 mol%. The major cellular phospholipids were phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SL6-1T formed a phylogenetic lineage within the genus Virgibacillus. Based on 16S rRNA gene sequence similarity, the strain was most closely related to Virgibacillus olivae E308T, Virgibacillus kekensis YIM kkny16T, Virgibacillus marismortui DSM 12325T with 97.1%, 97.1%, and 97.0% gene sequence similarities, respectively and the sequence similarities to other related taxa were less than 96.7%. The DNA relatedness values between strain SL6-1T and V. olivae E308T, V. kekensis YIM kkny16T, V. marismortui DSM 12325T were 16.7%, 51.0%, and 22.8%, respectively. On the basis of physiological, biochemical and phylogenetic properties, strain SL6-1T represents a novel species, for which the name Virgibacillus xinjiangensis sp. nov. is proposed. The type strain is SL6-1T (=KCTC 13128T =DSM 19031T).

Toxic Effects of Catechol and 4-Chlorobenzoate Stresses on Bacterial Cells: Sang-Ho Park , Yeon-Ja Ko , Chi-Kyung Kim; J. Microbiol. 2001;39(3):206-212.

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Abstract: Catechol and 4-chlorobenzoate (4CBA) which are produced from the biodegradation of a variety of aromatic and chloroaromatics have been recognized as toxic to living organisms. In this study, the toxic effects of catechol and 4-chlorobenzoate on gram-positive and -negative bacteria were examined in terms of survival, morphology, change in fatty acids and membrane protein composition. The survival rate of the organisms during treatment for 6 h was decreased, as the concentration of each aromatic was increased. Escherichia coli and Pseudomonas cells treated with catechol and 4CBA at concentrations causing a significant decrease in their viability, showed destructive openings in their cell envelopes. Bacillus subtilis treated with the aromatics were reduced in cell size and Staphylococcus aureus cells displayed irregular rod shapes with wrinkled surfaces. The bacterial cells treated with 20 mM catechol showed increases in unsaturated fatty acids, but several saturated fatty acids were decreased. In the E. coli cells treated with 20 mM catechol, inner membrane proteins of 150 kDa and 105 kDa were decreased. But several kinds of the inner and outer membrane proteins were increased. In B. subtilis treated with 20 mM catechol, several kinds of proteins were increased or decreased in membrane proteins.

Culture and Identification of Bacteria from Marine Biofilms: Yoo Kyung Lee , Kae-Kyung Kwon , Kyeung Hee Cho , Hyo Won Kim , Jae Hyun Park , Hong Kum Lee; J. Microbiol. 2003;41(3):183-188.

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Abstract: We isolated and cultured bacteria that inhabited marine biofilms, and identified them by phylogenetic analysis using 16S rDNA sequences. In the marine environment, biofilms cover most subtidal and intertidal solid surfaces such as rocks, ships, loops, marine animals, and algae. The bacteria in most biofilms are embedded in extracellular polymeric substances that comprise mainly of exopolysaccharides. The exopolysaccharides are excreted from multiple bacterial species; therefore, biofilms are a good source for screening exopolysaccharide-producing bacteria. Thirty-one strains were cultured, and a total of 17 unique strains were identified. Phylogenetic analysis using 16S rDNA sequences indicated that the 17 strains belonged to [alpha]-Proteobacteria (Ochrobactrum anthropi, Paracoccus carotinifaciens); [gamma]-Proteobacteria (Pseudoalteromonas agarovorans, P. piscicida, Pseudomonas aeruginosa, Shewanella baltica, Vibrio parahaemolyticus, V. pomeroyi); CFB group bacteria (Cytophaga latercula, Tenacibaculum mesophilum); high GC, Gram-positive bacteria (Arthrobacter nicotianae, Brevibacterium casei, B. epidermidis, Tsukamurella inchonensis); and low GC, Gram-positive bacteria (Bacillus macroides, Staphylococcus haemolyticus, S. warneri).

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