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- Taxonomic description and genome sequence of Halobacillus marinus sp. nov., a novel strain isolated from Chilika Lake, India
- Ananta N. Panda , Samir Ranjan Mishra , Lopamudra Ray , Surajit Das , Gurdeep Rastogi , Tapan Kumar Adhya , Mrutyunjay Suar , Vishakha Raina
- J. Microbiol. 2018;56(4):223-230. Published online April 2, 2018
- DOI: https://doi.org/10.1007/s12275-018-7387-x
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- 3 Crossref
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Abstract
- A moderately halophilic spore forming, motile, Gram-positive, rod-shaped bacterial strain designated as KGW1T was isolated from water sample of Chilika Lake and characterized taxonomically using polyphasic approach. The strain grew in the presence of 0–25% (w/v) NaCl in marine salt agar media, hydrolyzes casein, and gelatin and shows presence of alkaline proteases. The major cell wall menaquinone was MK7 and major cellular fatty acids were anteiso-C15:0 (44.89%), anteiso-C17:0 (6.18%), isoC15:0 (19.38%), and iso-C16:0 (7.39%). Several chemotaxonomic features conform the isolate be a member of genus Halobacillus. The isolate KGW1T contained A1γ meso-Dpm-direct type of peptidoglycan which is different from its phylogenetically closest neighbours. The 16S rRNA gene sequence based phylogenetic analysis also revealed the strain KGW1T was affiliated to the genus Halobacillus and sequence similarity between the isolated strain and the type strains of Halobacillus species were found closest to, H. dabanensis D-8 DSM 18199T (99.08%) and H. faecis IGA7-4 DSM 21559T (99.01%), H. trueperi SL-5 DSM 10404T (98.94%). The in silico DDH showed that the values in a range of 14.2–17.5% with the most closest strain H. dabanensis D-8 DSM 18199T and other type strains of the genus Halobacillus for which whole genome sequence is reported. DNA-DNA relatedness between strain KGW1T and the closest type strain Halobacillus trueperi DSM 10404T was 11.75% (± 1.15). The draft genome sequence includes 3,683,819 bases and comprises of 3898 predicted coding sequences with a G + C content of 46.98%. Thus, the significant distinctiveness supported by phenotypic and genotypic data with its closest neighbors and other closely related species confirm the strain KGW1T to be classified as a novel species within the genus Halobacillus, for which the name Halobacillus marinus sp. nov. is proposed. The type strain is KGW1T (= DSM 29522 = JCM 30443).
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Draft genome sequence of
Halobacillus campisalis
strain ASL-17
Anushree Srivastava, Michael Christopher Macey, Terry J. McGenity, Karen Olsson-Francis, Frank J. Stewart
Microbiology Resource Announcements.2024;[Epub] CrossRef - Trachyspermum ammi seed extract-mediated Ag nanoparticles: an insight into its in vitro biopotency
Vikneshvar K. S., R Subashini, Anieya Israel, Karuvelan Murugan, Namitha Ramakrishnan
Biomass Conversion and Biorefinery.2023;[Epub] CrossRef - Identification of antibacterial metabolites produced by a marine bacterium Halobacillus marinus HMALI004
Sardar Ali, Runlin Cai, Hao Feng, Jianmin Xie, Yueling Zhang, Hui Wang
Journal of Applied Microbiology.2022; 133(5): 3030. CrossRef
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Draft genome sequence of
Halobacillus campisalis
strain ASL-17
Research Support, Non-U.S. Gov'ts
- Cloning and Characterization of a Na+/H+ Antiporter Gene of the Moderately Halophilic Bacterium Halobacillus aidingensis AD-6T
- Ya Jie Zou , Li Fu Yang , Lei Wang , Su Sheng Yang
- J. Microbiol. 2008;46(4):415-421. Published online August 31, 2008
- DOI: https://doi.org/10.1007/s12275-008-0009-2
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- 14 Scopus
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Abstract
- A gene encoding a Na+/H+ antiporter was obtained from the genome of Halobacillus aidingensis AD-6T, which was sequenced and designated as nhaH. The deduced amino acid sequence of the gene was 91% identical to the NhaH of H. dabanensis, and shared 54% identity with the NhaG of Bacillus subtilis. The cloned gene enable the Escherichia coli KNabc cell, which lack all of the major Na+/H+ antiporters, to grow in medium containing 0.2 M NaCl or 10 mM LiCl. The nhaH gene was predicted to encode a 43.5 kDa protein (403 amino acid residues) with 11 putative transmembrane regions. Everted membrane vesicles prepared from E. coli KNabc cells carrying NhaH exhibited Na+/H+ as well as Li+/H+ antiporter activity, which was pH-dependent with the highest activity at pH 8.0, and no K+ /H+ antiporter activity was detected. The deletion of hydrophilic C-terminal amino acid residues showed that the short C-terminal tail was vital for Na+/H+ antiporter activity.
- Protein Expression Analysis of Halobacillus dabanensis D-8T Subjected to Salt Shock
- De Qin Feng , Bo Zhang , Wei Dong Lu , Su Sheng Yang
- J. Microbiol. 2006;44(4):369-374.
- DOI: https://doi.org/2418 [pii]
- 37 View
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Abstract
- To investigate the mechanism of salt tolerance of gram-positive moderately halophilic bacteria, two-dimensional gel electrophoresis (2-D PAGE) was employed to achieve high resolution maps of proteins of Halobacillus dabanensis D-8T. Approximately 700 spots of proteins were identified from these 2-D PAGE maps. The majority of these proteins had molecular weights between 17.5 and 66 kDa, and most of them were distributed between the isoelectric points (pI) 4.0 and 5.9. Some protein spots were distributed in the more acidic region of the 2-D gel (pI <4.0). This pattern indicated that a number of proteins in the strain D-8T are acidic. To understand the adaptation mechanisms of moderately halophilic bacteria in response to sudden environmental changes, differential protein profiles of this strain were investigated by 2-D PAGE and ImagemasterTM 2D Platinum software after the cells were subjected to salt shock of 1 to 25% salinity for 5 and 50 min. Analysis showed 59 proteins with an altered level of expression as the result of the exposure to salt shock. Eighteen proteins had increased expression, 8 proteins were induced, and the expression of 33 proteins was down-regulated. Eight of the up-regulated proteins were identified using MALDI-TOF/MS and MASCOT, and were similar to proteins involved in signal transduction, proteins participating in energy metabolism pathways and proteins involved in stress.
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