Journal of Microbiology

Journal Article

Halomonas tibetensis sp. nov., isolated from saline lakes on Tibetan Plateau: Hui-bin Lu , Peng Xing , Lei Zhai , Dorji Phurbu , Qian Tang , Qing-long Wu; J. Microbiol. 2018;56(7):493-499. Published online June 14, 2018; DOI: https://doi.org/10.1007/s12275-018-8076-5

43 View
0 Download
6 Crossref

Strains pyc13T and ZGT13 were isolated from Lake Pengyan and Lake Zigetang on Tibetan Plateau, respectively. Both strains were Gram-negative, catalase- and oxidase-positive, aerobic, rod-shaped, nonmotile, and nonflagellated bacteria. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains pyc13T and ZGT13 belong to the genus Halomonas, with Halomonas alkalicola 56-L4-10aEnT as their closest neighbor, showing 97.4% 16S rRNA gene sequence similarity. The predominant respiratory quinone of both strains was Q-9, with Q-8 as a minor component. The major fatty acids of both strains were C18:1 ω6c/C18:1 ω7c, C16:1 ω6c/C16:1 ω7c, C16:0, and C12:0 3OH. The polar lipids of both strains consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, glycolipid, phospholipids of unknown structure containing glucosamine, and unidentified phospholipids. The DNA G + C content of pyc13T and ZGT13 were 62.6 and 63.4 mol%, respectively. The DNA-DNA hybridization values of strain pyc13T were 34, 41, 61, 35, and 35% with the reference strains H. alkalicola 56-L4-10aEnT, H. sediminicola CPS11T, H. mongoliensis Z-7009T, H. ventosae Al12T, and H. fontilapidosi 5CRT, respectively. Phenotypic, biochemical, genotypic, and DNA-DNA hybridization data showed that strains pyc13T and ZGT13 represent a new species within the genus Halomonas, for which the name H. tibetensis sp. nov. is proposed. The type strain is pyc13T (= CGMCC 1.15949T = KCTC 52660T).

Citations

Citations to this article as recorded by

The Bacterial Microbiota of Artisanal Cheeses from the Northern Caucasus
Tatiana V. Kochetkova, Ilya P. Grabarnik, Alexandra A. Klyukina, Kseniya S. Zayulina, Liliya A. Gavirova, Polina A. Shcherbakova, Gennady S. Kachmazov, Andrey I. Shestakov, Ilya V. Kublanov, Alexander G. Elcheninov
Fermentation.2023; 9(8): 719. CrossRef
Halomonas profundi sp. nov., isolated from deep-sea sediment of the Mariana Trench
Fan Wang, Jin-Jian Wan, Xi-Ying Zhang, Yu Xin, Mei-Ling Sun, Peng Wang, Wei-Peng Zhang, Ji-Wei Tian, Yu-Zhong Zhang, Chun-Yang Li, Hui-Hui Fu
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Halomonas jincaotanensis sp. nov., isolated from the Pamir Plateau degrading polycyclic aromatic hydrocarbon
Xuying Bu, Zhanfeng Xia, Zhanwen Liu, Min Ren, Chuanxing Wan, Lili Zhang
Archives of Microbiology.2022;[Epub] CrossRef
Halomonas rituensis sp. nov. and Halomonas zhuhanensis sp. nov., isolated from natural salt marsh sediment on the Tibetan Plateau
Peixin Gao, Huibin Lu, Peng Xing, Qinglong L. Wu
International Journal of Systematic and Evolutionary Microbiology .2020; 70(10): 5217. CrossRef
Halomonas montanilacus sp. nov., isolated from hypersaline Lake Pengyanco on the Tibetan Plateau
Huibin Lu, Peng Xing, Lei Zhai, Huabing Li, Qinglong Wu
International Journal of Systematic and Evolutionary Microbiology .2020; 70(4): 2859. CrossRef
List of new names and new combinations previously effectively, but not validly, published
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology.2018; 68(11): 3379. CrossRef

Research Support, Non-U.S. Gov'ts

Identification and Characterization of Ectoine Biosynthesis Genes and Heterologous Expression of the ectABC Gene Cluster from Halomonas sp. QHL1, a Moderately Halophilic Bacterium Isolated from Qinghai Lake: Derui Zhu , Jian Liu , Rui Han , Guoping Shen , Qifu Long , Xiaoxing Wei , Deli Liu; J. Microbiol. 2014;52(2):139-147. Published online February 1, 2014; DOI: https://doi.org/10.1007/s12275-014-3389-5

45 View
0 Download
16 Crossref

Abstract

The moderately halophilic bacterium Halomonas sp. QHL1 was identified as a member of the genus Halomonas by 16S rRNA gene sequencing. HPLC analysis showed that strain QHL1 synthesizes ectoine in its cytoplasm. The genes involved in the ectoine biosynthesis pathway were identified on the chromosome in the order ectABC. Subsequently, the ectB gene from this strain was amplified by PCR, and the entire ectABC gene cluster (3,580 bp) was cloned using genome walking. Analysis showed that the ectA (579 bp), ectB (1269 bp), and ectC (390 bp) genes were organized in a single transcriptional unit and were predicted to encode three peptides of 21.2 kDa, 46.4 kDa, and 14.7 kDa, respectively. Two putative promoters, a δ70-dependent promoter and a δ38-controlled promoter, as well as several conserved motifs with unknown function were identified. Individual ectA, ectB, and ectC genes, and the entire ectABC gene cluster were inserted into the expression plasmid pET-28a(+) to generate the recombinant plasmids pET-28a(+)-ectA, pET-28a(+)-ectB, pET-28a(+)-ectC and pET-28a(+)-ectABC, respectively. Heterologous expression of these proteins in Escherichia coli BL21 (DE3) was confirmed by SDS-PAGE. The recombinant E. coli strain BL21 (pET-28a (+)-ectABC) displayed a higher salt tolerance than native E. coli cells but produced far less ectoine than the wild-type QHL1 strain.

Citations

Citations to this article as recorded by

Biotechnological production of ectoine: current status and prospects
Jun Chen, Deliang Qiao, Tao Yuan, Yeyuan Feng, Pengjun Zhang, Xuejun Wang, Li Zhang
Folia Microbiologica.2024; 69(2): 247. CrossRef
Comparative genomic analysis of Halomonas campaniensis wild-type and ultraviolet radiation-mutated strains reveal genomic differences associated with increased ectoine production
Zhibo Wang, Yongzhen Li, Xiang Gao, Jiangwa Xing, Rong Wang, Derui Zhu, Guoping Shen
International Microbiology.2023; 26(4): 1009. CrossRef
Comparative Genome Analysis of a Novel Alkaliphilic Actinobacterial Species Nesterenkonia haasae
Shuang Wang, Lei Sun, Manik Prabhu Narsing Rao, Bao‑zhu Fang, Wen‑jun Li
Polish Journal of Microbiology.2022; 71(3): 453. CrossRef
Whole genome sequencing of the halophilic Halomonas qaidamensis XH36, a novel species strain with high ectoine production
Tiantian Zhang, Tianqi Cui, Yaning Cao, Yongzhen Li, Fenghui Li, Derui Zhu, Jiangwa Xing
Antonie van Leeuwenhoek.2022; 115(4): 545. CrossRef
Identification and characterization of an ectoine biosynthesis gene cluster from Aestuariispira ectoiniformans sp. nov., isolated from seawater
Ji Young Kang, Binna Lee, Jeong Ah Kim, Min-Soo Kim, Chul Ho Kim
Microbiological Research.2022; 254: 126898. CrossRef
Genome Sequence of Streptomyces sp. Strain GQFP Isolated from Soil Near the Roots of Pharmaceutical Plant Elaeagnus pungens
Jie Zhu, David A. Baltrus
Microbiology Resource Announcements.2022;[Epub] CrossRef
Enhanced production of ectoine from methane using metabolically engineered Methylomicrobium alcaliphilum 20Z
Sukhyeong Cho, Yun Seo Lee, Hanyu Chai, Sang Eun Lim, Jeong Geol Na, Jinwon Lee
Biotechnology for Biofuels and Bioproducts.2022;[Epub] CrossRef
Ectoine Production Using Novel Heterologous EctABCS. salarius from Marine Bacterium Salinicola salarius
Yue Su, Wenting Peng, Tong Wang, Yanhui Li, Luyu Zhao, Xinyu Wang, Ying Li, Ling Lin
Applied Sciences.2021; 11(15): 6873. CrossRef
High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium
Qi Zhao, Shannan Li, Peiwen Lv, Simian Sun, Cuiqing Ma, Ping Xu, Haijun Su, Chunyu Yang
Microbial Cell Factories.2019;[Epub] CrossRef
Analysis of Zobellella denitrificans ZD1 draft genome: Genes and gene clusters responsible for high polyhydroxybutyrate (PHB) production from glycerol under saline conditions and its CRISPR-Cas system
Yu-Wei Wu, Shih-Hung Yang, Myung Hwangbo, Kung-Hui Chu, Chih-Horng Kuo
PLOS ONE.2019; 14(9): e0222143. CrossRef
Engineering the Salt-Inducible Ectoine Promoter Region of Halomonas elongata for Protein Expression in a Unique Stabilizing Environment
Lisa Stiller, Erwin Galinski, Elisabeth Witt
Genes.2018; 9(4): 184. CrossRef
New Alpiniamides From Streptomyces sp. IB2014/011-12 Assembled by an Unusual Hybrid Non-ribosomal Peptide Synthetase Trans-AT Polyketide Synthase Enzyme
Constanze Paulus, Yuriy Rebets, Josef Zapp, Christian Rückert, Jörn Kalinowski, Andriy Luzhetskyy
Frontiers in Microbiology.2018;[Epub] CrossRef
Metabolic engineering of Escherichia coli for L-tryptophan production
V. B. Panichkin, V. A. Livshits, I. V. Biryukova, S. V. Mashko
Applied Biochemistry and Microbiology.2016; 52(9): 783. CrossRef
Draft Genome Sequence of Halomonas elongata Strain K4, an Endophytic Growth-Promoting Bacterium Enhancing Salinity Tolerance In Planta
Feras F. Lafi, Juan S. Ramirez-Prado, Intikhab Alam, Vladimir B. Bajic, Heribert Hirt, Maged M. Saad
Genome Announcements.2016;[Epub] CrossRef
High production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinant Escherichia coli
Yong-Zhi He, Jiao Gong, Hai-Ying Yu, Yong Tao, Shan Zhang, Zhi-Yang Dong
Microbial Cell Factories.2015;[Epub] CrossRef
Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis
Wei Chen, Shan Zhang, Peixia Jiang, Jun Yao, Yongzhi He, Lincai Chen, Xiwu Gui, Zhiyang Dong, Shuang-Yan Tang
Metabolic Engineering.2015; 30: 149. CrossRef

Halomonas alkalitolerans sp. nov., a Novel Moderately Halophilic Bacterium Isolated from Soda Meadow Saline Soil in Daqing, China: Shuang Wang , Qian Yang , Zhi-Hua Liu , Lei Sun , Dan Wei , Jun-Zheng Zhang , Jin-Zhu Song , Yun Wang , Jia Song , Jin-Xia Fan , Xian-Xin Meng , Wei Zhang; J. Microbiol. 2011;49(1):24-28. Published online March 3, 2011; DOI: https://doi.org/10.1007/s12275-011-0197-z

40 View
0 Download
9 Scopus

Abstract: A moderately halophilic bacterial strain 15-13T, which was isolated from soda meadow saline soil in Daqing City, Heilongjiang Province, China, was subjected to a polyphasic taxonomic study. The cells of strain 15-13T were found to be Gram-negative, rod-shaped, and motile. The required growth conditions for strain 15-13T were: 1-23% NaCl (optimum, 7%), 10-50°C (optimum, 35°C), and pH 7.0-11.0 (optimum, pH 9.5). The predominant cellular fatty acids were C18:1 ω7c (60.48%) and C16:0 (13.96%). The DNA G+C content was 67.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 15-13T clustered within a branch comprising species of the genus Halomonas. The closest phylogenetic neighbor of strain 15-13T was Halomonas pantelleriensis DSM 9661T (98.9% 16S rRNA gene sequence similarity). The level of DNA-DNA relatedness between the novel isolated strain and H. pantelleriensis DSM 9661T was 33.8%. On the basis of the phenotypic and phylogenetic data, strain 15-13T represents a novel species of the genus Halomonas, for which the name Halomonas alkalitolerans sp. nov. is proposed. The type strain for this novel species is 15-13T (=CGMCC 1.9129T =NBRC 106539T).

NOTE] Halomonas jeotgali sp. nov., a New Moderate Halophilic Bacterium Isolated from a Traditional Fermented Seafood: Min-Soo Kim , Seong Woon Roh , Jin-Woo Bae; J. Microbiol. 2010;48(3):404-410. Published online June 23, 2010; DOI: https://doi.org/10.1007/s12275-010-0032-y

24 View
0 Download
27 Scopus

Abstract: A moderate halophilic, Gram-negative, non-motile, rod-shape, and aerobe designated as strain HwaT was isolated from traditional fermented Korean seafood, which presented as a single cell or paired cells. Optimal growth occurred at 25°C in 10% (w/v) salts at pH 7.0-8.0; however, growth occurred in a temperature range of 10-32°C, a salts concentration of 5-25% (w/v) and pH 5.0-10.0. Tests for oxidase and catalase were positive. The cells produced poly-β-hydroxybutyric acid, but not exopolysaccharide. Based on the 16S rRNA gene sequence, not only was there low similarity between strain HwaT and all other species (94.1% similarity with H. subglaciescola DSM 4683T, 94.0% similarity with H. sulfidaeris Esulfide1T, 93.6% similarity with H. cerina SP4T and 93.0% similarity with H. halodurans DSM 5160T), but the phylogenetic analysis revealed that the isolate may be classified as a novel species belonging to the genus Halomonas in the class Gammaproteobacteria. The predominant fatty acids of strain HwaT were C18:1 ω7c, C16:0, C12:0 3-OH and C16:1 ω7c/C15:0 iso 2-OH. The DNA G+C content was calculated as 61.7 mol%. Based on phenotypic, genotypic, and phylogenetic characteristics, it is proposed that the strain designated as HwaT be assigned to the genus Halomonas as Halomonas jeotgali sp. nov. (=KCTC 22487T =JCM 15645T).

Cel8H, a Novel Endoglucanase from the Halophilic Bacterium Halomonas sp. S66-4: Molecular Cloning, Heterogonous Expression, and Biochemical Characterization: Xiaoluo Huang , Zongze Shao , Yuzhi Hong , Ling Lin , Chanjuan Li , Fei Huang , Hui Wang , Ziduo Liu; J. Microbiol. 2010;48(3):318-324. Published online June 23, 2010; DOI: https://doi.org/10.1007/s12275-009-0188-5

38 View
0 Download
28 Scopus

Abstract: A recombinant Escherichia coli clone expressing an endoglucanase was identified from a genomic library of the halophilic bacterium Halomonas sp. S66-4, and the enzyme was designated Cel8H. The cel8H gene consisted of 1,053 bp and encoded 350 amino acids sharing the highest identity of 48% to other known endoglucanases. The protein was expressed in E. coli BL21 (DE3) and purified to homogeneity. The purified recombinant enzyme had an optimal activity of 4.9 U/mg at pH 5 and 45°C toward the substrate carboxymethylcellulose. It exhibited extraordinary properties which differed from endoglucanases reported previously at the point of high salt tolerance above 5 M, simultaneously with high pH stability at pH 4-12 and high temperature stability at 40-60°C. Various substrate tests indicated that the enzyme hydrolyzes β-1,4-glucosidic bonds specifically.

Journal Article

Growth and Physiological Properties of Wild Type and Mutants of Halomonas subglaciescola DH-1 in Saline Environment: Hye Jeong Ryu , Yoo Jung Jeong , Doo Hyun Park; J. Microbiol. 2004;42(3):174-180.; DOI: https://doi.org/2093 [pii]

39 View
0 Download

Abstract: A halophilic bacterium was isolated from fermented seafood. The 16S rDNA sequence identity between the isolate and Halomonas subglaciescola AJ306801 was above 95%. The isolate that did not grow in the condition without NaCl or in the condition with other sodium (Na^+) or chloride ions (Cl^-) instead of NaCl was named H. subglaciescola DH-1. Two mutants capable of growing without NaCl were obtained by random mutagenesis, of which their total soluble protein profiles were compared with those of the wild type by two-dimensional electrophoresis. The external compatible solutes (betaine and choline) and cell extract of the wild type did not function as osmoprotectants, and these parameters within the mutants did not enhance their growth in the saline environment. In the proton translocation test, rapid acidification of the reactant was not detected for the wild type, but it was detected for the mutant in the condition without NaCl. From these results, we derived the hypothesis that NaCl may be absolutely required for the energy metabolism of H. subglaciescola DH-1 but not for its osmoregulation, and the mutants may have another modified proton translocation system that is independent of NaCl, except for those mutants with an NaCl-dependent system.

First
Prev
Page of 1
Next
Last

Search