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Meta-Analysis
Proposal of a health gut microbiome index based on a meta-analysis of Korean and global population datasets
Hyun-Seok Oh , Uigi Min , Hyejin Jang , Namil Kim , Jeongmin Lim , Mauricio Chalita , Jongsik Chun
J. Microbiol. 2022;60(5):533-549.   Published online March 31, 2022
DOI: https://doi.org/10.1007/s12275-022-1526-0
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  • 9 Web of Science
  • 9 Crossref
AbstractAbstract
The disruption of the human gut microbiota has been linked to host health conditions, including various diseases. However, no reliable index for measuring and predicting a healthy microbiome is currently available. Here, the sequencing data of 1,663 Koreans were obtained from three independent studies. Furthermore, we pooled 3,490 samples from public databases and analyzed a total of 5,153 fecal samples. First, we analyzed Korean gut microbiome covariates to determine the influence of lifestyle on variation in the gut microbiota. Next, patterns of microbiota variations across geographical locations and disease statuses were confirmed using a global cohort and disease data. Based on comprehensive comparative analysis, we were able to define three enterotypes among Korean cohorts, namely, Prevotella type, Bacteroides type, and outlier type. By a thorough categorization of dysbiosis and the evaluation of microbial characteristics using multiple datasets, we identified a wide spectrum of accuracy levels in classifying health and disease states. Using the observed microbiome patterns, we devised an index named the gut microbiome index (GMI) that could consistently predict health conditions from human gut microbiome data. Compared to ecological metrics, the microbial marker index, and machine learning approaches, GMI distinguished between healthy and non-healthy individuals with a higher accuracy across various datasets. Thus, this study proposes a potential index to measure health status of gut microbiome that is verified from multiethnic data of various diseases, and we expect this model to facilitate further clinical application of gut microbiota data in future.

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  • A comparison of the prevalence of respiratory pathogens and opportunistic respiratory pathogenic profile of ‘clean’ and ‘unclean’ removable dental prostheses
    Tong Wah Lim, Shi Huang, Yufeng Zhang, Michael Francis Burrow, Colman McGrath
    Journal of Dentistry.2024; 145: 104968.     CrossRef
  • Characterization of pathogenic microbiome on removable prostheses with different levels of cleanliness using 2bRAD-M metagenomic sequencing
    Tong Wah Lim, Shi Huang, Yuesong Jiang, Yufeng Zhang, Michael Francis Burrow, Colman McGrath
    Journal of Oral Microbiology.2024;[Epub]     CrossRef
  • Gut microbial signatures in clinically stable ulcerative colitis according to the mucosal state and associated symptoms
    Soyoung Kim, Yeonjae Jung, Seung Bum Lee, Hyun‐Seok Oh, Sung Noh Hong
    Journal of Gastroenterology and Hepatology.2024; 39(2): 319.     CrossRef
  • Difference in gut microbial dysbiotic patterns between body-first and brain-first Parkinson's disease
    Don Gueu Park, Woorim Kang, In-Ja Shin, Mauricio Chalita, Hyun-Seok Oh, Dong-Wook Hyun, Hyun Kim, Jongsik Chun, Young-Sil An, Eun Jeong Lee, Jung Han Yoon
    Neurobiology of Disease.2024; 201: 106655.     CrossRef
  • Should Routine Diagnostics Implement Gut Microbiota Analysis?
    Giuseppe Guido Maria Scarlata, Ludovico Abenavoli
    The International Journal of Gastroenterology and Hepatology Diseases.2024;[Epub]     CrossRef
  • Feasibility study for a fully decentralized clinical trial in participants with functional constipation symptoms
    Ki Young Huh, Woo Kyung Chung, Jiyeon Park, SeungHwan Lee, Min‐Gul Kim, Jaeseong Oh, Kyung‐Sang Yu
    Clinical and Translational Science.2023; 16(11): 2177.     CrossRef
  • Predicting Personalized Responses to Dietary Fiber Interventions: Opportunities for Modulation of the Gut Microbiome to Improve Health
    Car Reen Kok, Devin Rose, Robert Hutkins
    Annual Review of Food Science and Technology.2023; 14(1): 157.     CrossRef
  • Effects of the multidomain intervention with nutritional supplements on cognition and gut microbiome in early symptomatic Alzheimer’s disease: a randomized controlled trial
    Eun Hye Lee, Geon Ha Kim, Hee Kyung Park, Hae Jin Kang, Yoo Kyoung Park, Hye Ah Lee, Chang Hyung Hong, So Young Moon, Woorim Kang, Hyun-Seok Oh, Hai-Jeon Yoon, Seong Hye Choi, Jee Hyang Jeong
    Frontiers in Aging Neuroscience.2023;[Epub]     CrossRef
  • Fecal microbial signatures of healthy Han individuals from three bio-geographical zones in Guangdong
    Litao Huang, Liting Deng, Changhui Liu, Enping Huang, Xiaolong Han, Cheng Xiao, Xiaomin Liang, Huilin Sun, Chao Liu, Ling Chen
    Frontiers in Microbiology.2022;[Epub]     CrossRef
Journal Articles
Description of Microbacterium luteum sp. nov., Microbacterium cremeum sp. nov., and Microbacterium atlanticum sp. nov., three novel C50 carotenoid producing bacteria
Fuquan Xie , Siwen Niu , Xihuang Lin , Shengxiang Pei , Li Jiang , Yun Tian , Gaiyun Zhang
J. Microbiol. 2021;59(10):886-897.   Published online September 7, 2021
DOI: https://doi.org/10.1007/s12275-021-1186-5
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AbstractAbstract
We have identified three Microbacterium strains, A18JL200T, NY27T, and WY121T, that produce C50 carotenoids. Taxonomy shows they represent three novel species. These strains shared < 98.5% 16S rRNA gene sequence identity with each other and were closely related to Microbacterium aquimaris JCM 15625T, Microbacterium yannicii JCM 18959T, Microbacterium ureisolvens CFH S00084T, and Microbacterium hibisci CCTCC AB 2016180T. Digital DNA-DNA hybridization (dDDH) values and average nucleotide identity (ANI) showed differences among the three strains and from their closest relatives, with values ranging from 20.4% to 34.6% and 75.5% to 87.6%, respectively. These values are below the threshold for species discrimination. Both morphology and physiology also differed from those of phylogenetically related Microbacterium species, supporting that they are indeed novel species. These strains produce C50 carotenoids (mainly decaprenoxanthin). Among the three novel species, A18JL200T had the highest total yield in carotenoids (6.1 mg/L or 1.2 mg/g dry cell weight). Unusual dual isoprenoid biosynthetic pathways (methylerythritol phosphate and mevalonate pathways) were annotated for strain A18JL200T. In summary, we found strains of the genus Microbacterium that are potential producers of C50 carotenoids, but their genome has to be investigated further.

Citations

Citations to this article as recorded by  
  • Identification, evaluation, control of yellow-causing bacteria and their influence on the quality of high moisture rice-based food
    Sijie Zhang, Lin Ou, Cuiping Yi, Qiutao Xie, Yang Shan
    Food Control.2025; 168: 110887.     CrossRef
  • Chlamydomonas reinhardtii and Microbacterium forte sp. nov., a mutualistic association that favors sustainable hydrogen production
    Neda Fakhimi, María Jesus Torres, Emilio Fernández, Aurora Galván, Alexandra Dubini, David González-Ballester
    Science of The Total Environment.2024; 913: 169559.     CrossRef
  • Discovery of a new bacterium, Microbacterium betulae sp. nov., in birch wood associated with hypersensitivity pneumonitis in woodworkers
    Mariola Paściak, Krzysztof J. Pawlik, Dariusz Martynowski, Łukasz Łaczmański, Jarosław Ciekot, Bogumiła Szponar, Angelina Wójcik‐Fatla, Barbara Mackiewicz, Ewelina Farian, Grażyna Cholewa, Alicja Cholewa, Jacek Dutkiewicz
    Environmental Microbiology Reports.2024;[Epub]     CrossRef
  • Microbacterium aquilitoris sp. nov. and Microbacterium gwkjiense sp. nov., isolated from beach
    Soon Dong Lee, Hong Lim Yang, Ji-Sun Kim, In Seop Kim
    Archives of Microbiology.2024;[Epub]     CrossRef
  • Microbacterium algihabitans sp. nov., Microbacterium phycohabitans sp. nov., and Microbacterium galbum sp. nov., isolated from dried beach seaweeds
    Soon Dong Lee, Hong Lim Yang, In Seop Kim
    International Journal of Systematic and Evolutionary Microbiology .2024;[Epub]     CrossRef
  • Association between the gut microbiome and carotenoid plumage phenotype in an avian hybrid zone
    Marcella D. Baiz, Andrew W. Wood, David P. L. Toews
    Proceedings of the Royal Society B: Biological Sciences.2024;[Epub]     CrossRef
  • Description of Microbacterium dauci sp. nov., a plant growth hormone indoleacetic acid-producing and nitrogen-fixing bacterium isolated from carrot rhizosphere soil
    Jiawen Zheng, Yunyu Liao, Yang Li, Dongsheng Li, Yaping Sun, Zijun Xiao
    Archives of Microbiology.2024;[Epub]     CrossRef
  • Can Sugarcane Yield and Health Be Altered with Fully Mechanized Management?
    Jian Xiao, Tian Liang, Shangdong Yang, Hongwei Tan
    Agronomy.2023; 13(1): 153.     CrossRef
  • Evaluation of Various Escherichia coli Strains for Enhanced Lycopene Production
    Jun Ren, Junhao Shen, Thi Duc Thai, Min-gyun Kim, Seung Ho Lee, Wonseop Lim, Dokyun Na
    Journal of Microbiology and Biotechnology.2023; 33(7): 973.     CrossRef
  • Effects of flavonoids on membrane adaptation of food-associated bacteria
    Maria Linden, Alexander Flegler, Michelle M. Feuereisen, Fabian Weber, André Lipski, Andreas Schieber
    Biochimica et Biophysica Acta (BBA) - Biomembranes.2023; 1865(4): 184137.     CrossRef
  • Four new Microbacterium species isolated from seaweeds and reclassification of five Microbacterium species with a proposal of Paramicrobacterium gen. nov. under a genome-based framework of the genus Microbacterium
    Soon Dong Lee, Hong Lim Yang, In Seop Kim
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Microbacterium Cluster EA Bacteriophages: Phylogenomic Relationships and Host Range Predictions
    Mark Milhaven, Cyril J. Versoza, Aman Garg, Lindsey Cai, Sanjana Cherian, Kamalei Johnson, Kevin Salas Perez, Madison Blanco, Jackelyn Lobatos, Corinne Mitra, Maria Strasser, Susanne P. Pfeifer
    Microorganisms.2023; 11(1): 170.     CrossRef
  • Valid publication of new names and new combinations effectively published outside the IJSEM. Validation List no. 203
    Aharon Oren, George M. Garrity
    International Journal of Systematic and Evolutionary Microbiology .2022;[Epub]     CrossRef
The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus
Renfei Lu , Junfang Sun , Yue Qiu , Miaomiao Zhang , Xingfan Xue , Xue Li , Wenhui Yang , Dongsheng Zhou , Lingfei Hu , Yiquan Zhang
J. Microbiol. 2021;59(7):651-657.   Published online June 1, 2021
DOI: https://doi.org/10.1007/s12275-021-0629-3
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AbstractAbstract
Vibrio parahaemolyticus possesses two types of flagella: a single polar flagellum (Pof) for swimming and the peritrichous lateral flagella (Laf) for swarming. Expression of Laf genes has previously been reported to be regulated by the quorum sensing (QS) regulators AphA and OpaR. In the present study, we showed that OpaR, the QS regulator at high cell density (HCD), acted as a negative regulator of swimming motility and the transcription of Pof genes in V. parahaemolyticus. OpaR bound to the promoter-proximal DNA regions of flgAMN, flgMN, and flgBCDEFGHIJ within the Pof gene loci to repress their transcription, whereas it negatively regulates the transcription of flgKL-flaC in an indirect manner. Thus, this work investigated how QS regulated the swimming motility via direct action of its master regulator OpaR on the transcription of Pof genes in V. parahaemolyticus.

Citations

Citations to this article as recorded by  
  • H-NS-Mediated Regulation of Swimming Motility and Polar Flagellar Gene Expression in Vibrio parahaemolyticus
    Yue Zhou, Jingyang Chang, Feng Li, Mei He, Rui Li, Yaqin Hou, Yiquan Zhang, Renfei Lu, Ming Yang
    Current Microbiology.2025;[Epub]     CrossRef
  • GefB, a GGDEF domain-containing protein, affects motility and biofilm formation of Vibrio parahaemolyticus and is regulated by quorum sensing regulators
    Yining Zhou, Jingyang Chang, Miaomiao Zhang, Xue Li, Xi Luo, Wanpeng Li, Zhukang Tian, Nan Zhang, Bin Ni, Yiquan Zhang, Renfei Lu
    Gene.2025; 933: 148968.     CrossRef
  • The effect of environmental calcium on gene expression, biofilm formation and virulence of Vibrio parahaemolyticus
    Xue Li, Jingyang Chang, Miaomiao Zhang, Yining Zhou, Tingting Zhang, Yiquan Zhang, Renfei Lu
    Frontiers in Microbiology.2024;[Epub]     CrossRef
  • VPA0198, a GGDEF domain-containing protein, affects the motility and biofilm formation of Vibrio parahaemolyticus and is regulated by quorum sensing associated regulators
    Yining Zhou, Jingyang Chang, Miaomiao Zhang, Xue Li, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Bin Ni, Renfei Lu, Yiquan Zhang
    Microbial Pathogenesis.2024; 195: 106882.     CrossRef
  • Antibacterial and anti-virulence potential of plant phenolic compounds against Vibrio parahaemolyticus
    F. Javier Vazquez-Armenta, M. Olivia Aros-Corrales, M. Lizeth Alvarez-Ainza, A. Thalia Bernal-Mercado, J. Fernando Ayala-Zavala, Adrian Ochoa-Leyva, A. Alexis Lopez-Zavala
    F1000Research.2024; 12: 1256.     CrossRef
  • Environmental magnesium ion affects global gene expression, motility, biofilm formation and virulence of Vibrio parahaemolyticus
    Xue Li, Xiaobai Zhang, Miaomiao Zhang, Xi Luo, Tingting Zhang, Xianjin Liu, Renfei Lu, Yiquan Zhang
    Biofilm.2024; 7: 100194.     CrossRef
  • The histone-like nucleoid-structuring protein encoded by the plasmid pMBL6842 regulates both plasmid stability and host physiology of Pseudoalteromonas rubra SCSIO 6842
    Baiyuan Li, Songwei Ni, Yabo Liu, Jianzhong Lin, Xiaoxue Wang
    Microbiological Research.2024; 286: 127817.     CrossRef
  • The Impact of Vp-Porin, an Outer Membrane Protein, on the Biological Characteristics and Virulence of Vibrio Parahaemolyticus
    Jinyuan Che, Qitong Fang, Shaojie Hu, Binghong Liu, Lei Wang, Xiu Fang, Lekang Li, Tuyan Luo, Baolong Bao
    Biology.2024; 13(7): 485.     CrossRef
  • Phenotypic changes and gene expression profiles of Vibrio parahaemolyticus in response to low concentrations of ampicillin
    Xi Luo, Miaomiao Zhang, Yiquan Zhang, Xue Li, Renfei Lu
    The Journal of Antibiotics.2024; 77(12): 823.     CrossRef
  • Bioprospecting and Exploration of Phytochemicals as Quorum Sensing Inhibitors against Cariogenic Dental Biofilm
    S. Arya, R. Usha
    Journal of Pure and Applied Microbiology.2024; 18(1): 100.     CrossRef
  • Identification of an LysR family transcriptional regulator that activates motility and flagellar gene expression in Vibrio parahaemolyticus
    Jingyang Chang, Yining Zhou, Xue Li, Miaomiao Zhang, Yiquan Zhang, Bin Ni, Renfei Lu
    Letters in Applied Microbiology.2024;[Epub]     CrossRef
  • The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces
    Francis J. Santoriello, Bonnie L. Bassler, Ankur B. Dalia
    PLOS Genetics.2024; 20(7): e1011243.     CrossRef
  • Evaluation of Therapeutic Efficiency of Stylicin against Vibrio parahaemolyticus Infection in Shrimp Penaeus vannamei through Comparative Proteomic Approach
    Saranya Chakrapani, Akshaya Panigrahi, Esakkiraj Palanichamy, Sathish Kumar Thangaraj, Naveenkumar Radhakrishnan, Puspamitra Panigrahi, Radhakrishnan Nagarathnam
    Probiotics and Antimicrobial Proteins.2024; 16(1): 76.     CrossRef
  • CalR Inhibits the Swimming Motility and Polar Flagellar Gene Expression in Vibrio parahaemolyticus
    Jingyang Chang, Yining Zhou, Miaomiao Zhang, Xue Li, Nan Zhang, Xi Luo, Bin Ni, Haisheng Wu, Renfei Lu, Yiquan Zhang
    Journal of Microbiology.2024; 62(12): 1125.     CrossRef
  • Quorum sensing: An emerging role for Vibrio infection and host defense
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    Infectious Microbes and Diseases.2024;[Epub]     CrossRef
  • QsvR and OpaR coordinately repress biofilm formation by Vibrio parahaemolyticus
    Miaomiao Zhang, Xingfan Xue, Xue Li, Qimin Wu, Tingting Zhang, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Renfei Lu, Yiquan Zhang
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Transcriptomic Profiles of Vibrio parahaemolyticus During Biofilm Formation
    Yiquan Zhang, Tingting Zhang, Yue Qiu, Miaomiao Zhang, Xiuhui Lu, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Bo Gao, Renfei Lu
    Current Microbiology.2023;[Epub]     CrossRef
  • Antibacterial and anti-virulence potential of plant phenolic compounds against Vibrio parahaemolyticus
    F. Javier Vazquez-Armenta, M. Olivia Aros-Corrales, M. Lizeth Alvarez-Ainza, A. Thalia Bernal-Mercado, J. Fernando Ayala-Zavala, Adrian Ochoa-Leyva, A. Alexis Lopez-Zavala
    F1000Research.2023; 12: 1256.     CrossRef
  • Effect of sublethal dose of chloramphenicol on biofilm formation and virulence in Vibrio parahaemolyticus
    Miaomiao Zhang, Liyan Cai, Xi Luo, Xue Li, Tingting Zhang, Fei Wu, Yiquan Zhang, Renfei Lu
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • Quorum sensing and QsvR tightly control the transcription of vpa0607 encoding an active RNase II-type protein in Vibrio parahaemolyticus
    Yiquan Zhang, Xingfan Xue, Fengjun Sun, Xue Li, Miaomiao Zhang, Qimin Wu, Tingting Zhang, Xi Luo, Renfei Lu
    Frontiers in Microbiology.2023;[Epub]     CrossRef
  • QsvR represses the transcription of polar flagellum genes in Vibrio parahaemolyticus
    Miaomiao Zhang, Xingfan Xue, Xue Li, Xi Luo, Qimin Wu, Tingting Zhang, Wenhui Yang, Lingfei Hu, Dongsheng Zhou, Renfei Lu, Yiquan Zhang
    Microbial Pathogenesis.2023; 174: 105947.     CrossRef
  • Transcriptomic Analysis of Vibrio parahaemolyticus Underlying the Wrinkly and Smooth Phenotypes
    Qimin Wu, Xue Li, Tingting Zhang, Miaomiao Zhang, Xingfan Xue, Wenhui Yang, Lingfei Hu, Zhe Yin, Dongsheng Zhou, Yuyu Sun, Renfei Lu, Yiquan Zhang, Sébastien P. Faucher
    Microbiology Spectrum.2022;[Epub]     CrossRef
  • Gut microbiota analysis of Blenniidae fishes including an algae-eating fish and clear boundary formation among isolated Vibrio strains
    Masa-aki Yoshida, Takuma Tanabe, Hideo Akiyoshi, Makoto Kawamukai
    Scientific Reports.2022;[Epub]     CrossRef
  • Computationally Designed Anti-LuxP DNA Aptamer Suppressed Flagellar Assembly- and Quorum Sensing-Related Gene Expression in Vibrio parahaemolyticus
    Nur Afiqah Md Yusof, Siti Aisyah Razali, Azyyati Mohd Padzil, Benjamin Yii Chung Lau, Syarul Nataqain Baharum, Nor Azlan Nor Muhammad, Nurul Hanun Ahmad Raston, Chou Min Chong, Natrah Fatin Mohd Ikhsan, Magdalena Lenny Situmorang, Low Chen Fei
    Biology.2022; 11(11): 1600.     CrossRef
  • An Increase of Seawater Temperature Upregulates the Expression of Vibrio parahaemolyticus Virulence Factors Implicated in Adhesion and Biofilm Formation
    Mélanie Billaud, François Seneca, Eric Tambutté, Dorota Czerucka
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • Characterization of the RpoN regulon reveals the regulation of motility, T6SS2 and metabolism in Vibrio parahaemolyticus
    Dan Gu, Youkun Zhang, Kangru Wang, Mingzhu Li, Xinan Jiao
    Frontiers in Microbiology.2022;[Epub]     CrossRef
Comparative genomic analysis of selenium utilization traits in different marine environments
Muhammad Farukh
J. Microbiol. 2020;58(2):113-122.   Published online January 29, 2020
DOI: https://doi.org/10.1007/s12275-020-9250-0
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  • 3 Web of Science
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AbstractAbstract
Selenium (Se) is an essential trace element for many organisms, which is required in the biosynthesis of proteins with selenocysteine, tRNAs with selenouridine, and certain enzymes with Se as a cofactor. Recent large-scale metagenomics projects provide a unique opportunity for studying the global trends of Se utilization in marine environments. Here, we analyzed samples from different marine microbial communities, revealed by the Tara Oceans project, to characterize the Se utilization traits. We found that the selenophosphate synthetase gene, which defines the overall Se utilization, and Se utilization traits are present in all samples. Regions with samples rich and poor in Se utilization traits were categorized. From the analysis of environmental factors, the mesopelagic zone and high temperature (> 15°C) of water are favorable, while geographical location has little influence on Se utilization. All Se utilization traits showed a relatively independent occurrence. The taxonomic classification of Se traits shows that most of the sequences corresponding to Se utilization traits belong to the phylum Proteobacteria. Overall, our study provides useful insights into the general features of Se utilization in ocean samples and may help to understand the evolutionary dynamics of Se utilization in different marine environments.

Citations

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  • The selenophosphate synthetase family: A review
    Bruno Manta, Nadezhda E Makarova, Marco Mariotti
    Free Radical Biology and Medicine.2022; 192: 63.     CrossRef
  • Selenium Metabolism and Selenoproteins in Prokaryotes: A Bioinformatics Perspective
    Yan Zhang, Jiao Jin, Biyan Huang, Huimin Ying, Jie He, Liang Jiang
    Biomolecules.2022; 12(7): 917.     CrossRef
  • Uses of Selenium Nanoparticles in the Plant Production
    Iqra Bano, Sylvie Skalickova, Hira Sajjad, Jiri Skladanka, Pavel Horky
    Agronomy.2021; 11(11): 2229.     CrossRef
Research Support, Non-U.S. Gov'ts
Distinct Patterns of Marine Bacterial Communities in the South and North Pacific Oceans
Sung-Suk Suh , Mirye Park , Jinik Hwang , Sukchan Lee , Youngjae Chung , Taek-Kyun Lee
J. Microbiol. 2014;52(10):834-841.   Published online October 1, 2014
DOI: https://doi.org/10.1007/s12275-014-4287-6
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AbstractAbstract
The study of oceanic microbial communities is crucial for our understanding of the role of microbes in terms of biomass, diversity and ecosystem function. In this study, 16S rRNA gene tag pyrosequencing was used to investigate change in bacterial community structure between summer and winter water masses from Gosung Bay in the South Sea of Korea and Chuuk in Micronesia, located in the North and South Pacific Oceans, respectively. Summer and winter sampling from each water mass revealed highly diverse bacterial communities, containing ~900 Operational Taxonomic Units (OTUs). The microbial distribution and highly heterogeneous composition observed at both sampling sites were different from those of most macroorganisms. The bacterial communities in the seawater at both sites were most abundant in Proteobacteria during the summer in Gosung and in Bacterioidetes during the winter. The proportion of Cyanobacteria was higher in summer than in winter in Chuuk and similar in Gosung. Additionally, the microbial community during summer in Gosung was significantly different from other communities observed based on the unweighted UniFrac distance. These data suggest that in both oceanic areas sampled, the bacterial communities had distinct distribution patterns with spatially- and temporally-heterogeneous distributions.

Citations

Citations to this article as recorded by  
  • Metavirome Profiling and Dynamics of the DNA Viral Community in Seawater in Chuuk State, Federated States of Micronesia
    Seung Won Jung, Kang Eun Kim, Hyun-Jung Kim, Taek-Kyun Lee
    Viruses.2023; 15(6): 1293.     CrossRef
  • The Diversity and Metabolism of Culturable Nitrate-Reducing Bacteria from the Photic Zone of the Western North Pacific Ocean
    Zhichen Jiang, Sizhen Liu, Dechao Zhang, Zhongli Sha
    Microbial Ecology.2023; 86(4): 2781.     CrossRef
  • Exploring the plankton bacteria diversity and distribution patterns in the surface water of northwest pacific ocean by metagenomic methods
    Yafei Wang, Hongmei Lin, Ranran Huang, Weidong Zhai
    Frontiers in Marine Science.2023;[Epub]     CrossRef
  • Characterization of bacterial community structure in two alcyonacean soft corals (Litophyton sp. and Sinularia sp.) from Chuuk, Micronesia
    Joon Sang Park, Jeonghoon Han, Sung-Suk Suh, Hyun-Jung Kim, Taek-Kyun Lee, Seung Won Jung
    Coral Reefs.2022; 41(3): 563.     CrossRef
  • Influences of Seasonal Monsoons on the Taxonomic Composition and Diversity of Bacterial Community in the Eastern Tropical Indian Ocean
    Ping Gao, Guangxun Du, Duo Zhao, Qinsheng Wei, Xuelei Zhang, Lingyun Qu, Xianzhe Gong
    Frontiers in Microbiology.2021;[Epub]     CrossRef
  • Bacterial and Fungal Diversity in Sediment and Water Column From the Abyssal Regions of the Indian Ocean
    Natasha Maria Barnes, Samir R. Damare, Belle Damodara Shenoy
    Frontiers in Marine Science.2021;[Epub]     CrossRef
  • Diversity and biogeography of picoplankton communities from the Straits of Malacca to the South China Sea
    Zhao-Yu Jiang, Fu-Lin Sun
    Oceanological and Hydrobiological Studies.2020; 49(1): 23.     CrossRef
  • Abundance and community composition of bacterioplankton in the Northern South China Sea during winter: geographic position and water layer influences
    Rongjun Shi, Jiajun Li, Zhanhui Qi, Zhe Zhang, Huaxue Liu, Honghui Huang
    Biologia.2018; 73(2): 197.     CrossRef
  • Bacterial Diversity and Nitrogen Utilization Strategies in the Upper Layer of the Northwestern Pacific Ocean
    Yuan-Yuan Li, Xiao-Huang Chen, Zhang-Xian Xie, Dong-Xu Li, Peng-Fei Wu, Ling-Fen Kong, Lin Lin, Shuh-Ji Kao, Da-Zhi Wang
    Frontiers in Microbiology.2018;[Epub]     CrossRef
  • Distribution Patterns of Microbial Community Structure Along a 7000-Mile Latitudinal Transect from the Mediterranean Sea Across the Atlantic Ocean to the Brazilian Coastal Sea
    Jin Zhou, Xiao Song, Chun-Yun Zhang, Guo-Fu Chen, Yong-Min Lao, Hui Jin, Zhong-Hua Cai
    Microbial Ecology.2018; 76(3): 592.     CrossRef
  • A snapshot on spatial and vertical distribution of bacterial communities in the eastern Indian Ocean
    Jing Wang, Jinjun Kan, Laura Borecki, Xiaodong Zhang, Dongxiao Wang, Jun Sun
    Acta Oceanologica Sinica.2016; 35(6): 85.     CrossRef
  • Seasonal Dynamics of Marine Microbial Community in the South Sea of Korea
    Sung-Suk Suh, Mirye Park, Jinik Hwang, Eui-Joon Kil, Seung Won Jung, Sukchan Lee, Taek-Kyun Lee, Torsten Thomas
    PLOS ONE.2015; 10(6): e0131633.     CrossRef
Effect of Fumarate Reducing Bacteria on In Vitro Rumen Fermentation, Methane Mitigation and Microbial Diversity
Lovelia Mamuad , Seon Ho Kim , Chang Dae Jeong , Yeon Jae Choi , Che Ok Jeon , Sang-Suk Lee
J. Microbiol. 2014;52(2):120-128.   Published online February 1, 2014
DOI: https://doi.org/10.1007/s12275-014-3518-1
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AbstractAbstract
The metabolic pathways involved in hydrogen (H2) production, utilization and the activity of methanogens are the important factors that should be considered in controlling methane (CH4) emissions by ruminants. H2 as one of the major substrate for CH4 production is therefore should be controlled. One of the strategies on reducing CH4 is through the use of hydrogenotrophic microorganisms such as fumarate reducing bacteria. This study determined the effect of fumarate reducing bacteria, Mitsuokella jalaludinii, supplementation on in vitro rumen fermentation, CH4 production, diversity and quantity. M. jalaludinii significantly reduced CH4 at 48 and 72 h of incubation and significantly increased succinate at 24 h. Although not significantly different, propionate was found to be highest in treatment containing M. jalaludinii at 12 and 48 h of incubation. These results suggest that supplementation of fumarate reducing bacteria to ruminal fermentation reduces CH4 production and quantity, increases succinate and changes the rumen microbial diversity.

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Molecular Detection and Genotyping of Fusarium oxysporum f. sp. psidii Isolates from Different Agro-Ecological Regions of India
Rupesh Kumar Mishra , Brajesh Kumar Pandey , Vijai Singh , Amita John Mathew , Neelam Pathak , Mohammad Zeeshan
J. Microbiol. 2013;51(4):405-412.   Published online August 30, 2013
DOI: https://doi.org/10.1007/s12275-013-2638-3
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AbstractAbstract
Twenty one isolates of Fusarium oxysporum f. sp. psidii (Fop), causing a vascular wilt in guava (Psidium guajava L.), were collected from different agro-ecological regions of India. The pathogenicity test was performed in guava seedlings, where the Fop isolates were found to be highly pathogenic. All 21 isolates were confirmed as F. oxysporum f. sp. psidii by a newly developed, species-specific primer against the conserved regions of 28S rDNA and the intergenic spacer region. RAPD and PCR-RFLP were used for genotyping the isolates to determine their genetic relationships. Fifteen RAPD primers were tested, of which five primers produced prominent, polymorphic, and reproducible bands. RAPD yielded an average of 6.5 polymorphic bands per primer, with the amplified DNA fragments ranging from 200–2,000 bp in size. A dendrogram constructed from these data indicated a 22–74% level of homology. In RFLP analysis, two major bands (350 and 220 bp) were commonly present in all isolates of F. oxysporum. These findings provide new insight for rapid, specific, and sensitive disease diagnosis. However, genotyping could be useful in strain-level discrimination of isolates from different agro-ecological regions of India.
Novel and Highly Diverse Fungal Endophytes in Soybean Revealed by the Consortium of Two Different Techniques
Tiago de Souza Leite , Andréia Cnossen-Fassoni , Olinto Liparini Pereira , Eduardo Seiti Gomide Mizubuti , Elza Fernandes de Araújo , Marisa Vieira de Queiroz
J. Microbiol. 2013;51(1):56-69.   Published online March 2, 2013
DOI: https://doi.org/10.1007/s12275-013-2356-x
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  • 40 Scopus
AbstractAbstract
Fungal endophytes were isolated from the leaves of soybean cultivars in Brazil using two different isolation techniques – fragment plating and the innovative dilution-to-extinction culturing – to increase the species richness, frequency of isolates and diversity. A total of 241 morphospecies were obtained corresponding to 62 taxa that were identified by analysis of the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA). The Phylum Ascomycota predominated, representing 99% and 95.2% of isolates in the Monsoy and Conquista cultivars, respectively, whereas the Phylum Basidiomycota represented 1% and 4.8% of isolates, respectively. The genera Ampelomyces, Annulohypoxylon, Guignardia, Leptospora, Magnaporthe, Ophiognomonia, Paraconiothyrium, Phaeosphaeriopsis, Rhodotorula, Sporobolomyces, and Xylaria for the first time were isolated from soybean; this suggests that soybean harbours novel and highly diverse fungi. The yeasts genera Rhodotorula and Sporobolomyces (subphylum Pucciniomycotina) represent the Phylum Basidiomycota. The species richness was greater when both isolation techniques were used. The diversity of fungal endophytes was similar in both cultivars when the same isolation technique was used except for Hill’s index, N1. The use of ITS region sequences allowed the isolates to be grouped according to Order, Class and Phylum. Ampelomyces, Chaetomium, and Phoma glomerata are endophytic species that may play potential roles in the biological control of soybean pathogens. This study is one of the first to apply extinction-culturing to isolate fungal endophytes in plant leaves, thus contributing to the development and improvement of this technique for future studies.
NOTE] Molecular Phylogenetic Status of Korean Strain of Podosphaera xanthii, a Causal Pathogen of Powdery Mildew on Japanese Thistle (Cirsium japonicum) in Korea
Hyang Burm Lee
J. Microbiol. 2012;50(6):1075-1080.   Published online December 30, 2012
DOI: https://doi.org/10.1007/s12275-012-2618-z
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AbstractAbstract
Powdery mildew diseases are sensitive to climate change and spread can be favored by increased temperature and low moisture. During 2011 to 2012, a powdery mildew disease by a Podosphaera species was observed on the leaves of Japanese thistle (Cirsium japonicum) in Korea. The initial sign of this disease included scattered superficial white mycelia on leaves. As the disease progressed, abundant necrotic black spots exhibiting chasmothecia were formed on the leaves. rDNA ITS and 28S homologies of the fungus (EML-CSPW1) showed 100% identity values with those regions from many strains of P. xanthii (syn. P. fusca) via NCBI BLASTN search.
Characterization, Metabolites and Gas Formation of Fumarate Reducing Bacteria Isolated from Korean Native Goat (Capra hircus coreanae)
Lovelia L. Mamuad , Seon Ho Kim , Sung Sil Lee , Kwang Keun Cho , Che Ok Jeon , Sang-Suk Lee
J. Microbiol. 2012;50(6):925-931.   Published online December 30, 2012
DOI: https://doi.org/10.1007/s12275-012-2497-3
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AbstractAbstract
Fumarate reducing bacteria, able to convert fumarate to succinate, are possible to use for the methane reduction in rumen because they can compete for H2 with methanogens. In this, we isolated fumarate reducing bacteria from a rumen of Korean native goat and characterized their molecular properties [fumarate reductase A gene (frdA)], fumarate reductase activities, and productions of volatile fatty acids and gas. Eight fumarate reducing bacteria belonging to Firmicutes were isolated from rumen fluid samples of slaughtered Korean black goats and characterized their phylogenetic positions based on 16S rRNA gene sequences. PCR based analyses showed that only one strain, closely related to Mitsuokella jalaludinii, harbored frdA. The growths of M. jalaludinii and Veillonella parvula strains were tested for different media. Interestingly, M. jalaludinii grew very well in the presence of hydrogen alone, while V. parvula grew well in response of fumarate and fumarate plus hydrogen. M. jalaludinii produced higher levels of lactate (P≤0.05) than did V. parvula. Additionally, M. jalaludinii produced acetate, but not butyrate, whereas V. parvula produced butyrate, not acetate. The fumarate reductase activities of M. jalaludinii and V. parvula were 16.8 ± 0.34 and 16.9 ± 1.21 mmol NADH oxidized/min/mg of cellular N, respectively. In conclusion, this showed that M. jalaludinii can be used as an efficient methane reducing agent in rumen.
NOTE] Detection of a Unique Fibrinolytic Enzyme in Aeromonas sp. JH1
Han-Young Cho , Min Jeong Seo , Jeong Uck Park , Byoung Won Kang , Gi-Young Kim , Woo Hong Joo , Young-Choon Lee , Yong Kee Jeong
J. Microbiol. 2011;49(6):1018-1021.   Published online December 28, 2011
DOI: https://doi.org/10.1007/s12275-011-1376-7
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AbstractAbstract
A fibrinolytic enzyme was found in a Gram-negative bacterium, Aeromonas sp. JH1. SDS-PAGE and fibrinzymography showed that it was a 36 kDa, monomeric protein. Of note, the enzyme was highly specific for fibrinogen molecules and the hydrolysis rate of fibrinogen subunits was highest for α, β, and γ chains in that order. The first 15 amino acids of N-terminal sequence were X-D-A-T-G-P-G-G-N-V-X-T-G-K-Y, which was distinguishable from other fibrinolytic enzymes. The optimum pH and temperature of the enzyme were approximately 8.0 and 40°C, respectively. Therefore, these results provide a fibrinolytic enzyme with potent thrombolytic activity from the Aeromonas genus.
Diversity of Endophytic Bacteria in Ginseng and Their Potential for Plant Growth Promotion
Regupathy Thamizh Vendan , Young Joon Yu , Sun Hee Lee , Young Ha Rhee
J. Microbiol. 2010;48(5):559-565.   Published online November 3, 2010
DOI: https://doi.org/10.1007/s12275-010-0082-1
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AbstractAbstract
Endophytic bacteria have been found in virtually every plant studied, where they colonize the internal tissues of their host plant and can form a range of different beneficial relationships. The diversity of bacterial endophytes associated with ginseng plants of varying age levels in Korea was investigated. Fifty-one colonies were isolated from the interior of ginseng stems. Although a mixed composition of endophyte communities was recovered from ginseng based on the results of 16S rDNA analysis, bacteria of the genus Bacillus and Staphylococcus dominated in 1-year-old and 4-year-old plants, respectively. Phylogenetic analysis revealed four clusters: Firmicutes, Actinobacteria, α-Proteobacteria, and γ-Proteobacteria, with Firmicutes being predominant. To evaluate the plant growth promoting activities, 18 representative isolates were selected. Amplification of nifH gene confirmed the presence of diazotrophy in only two isolates. Half of the isolates solubilized mineral phosphate. Except four, all the other endophytic isolates produced significant amounts of indole acetic acid in nutrient broth. Iron sequestering siderophore production was detected in seven isolates. Isolates E-I-3 (Bacillus megaterium), E-I-4 (Micrococcus luteus), E-I-8 (B. cereus), and E-I-20 (Lysinibacillus fusiformis) were positive for most of the plant growth promoting traits, indicating their role in growth promotion of ginseng.
Journal Article
Characterization of Cultivated Fungi Isolated from Grape Marc Wastes Through the Use of Amplified rDNA Restriction Analysis and Sequencing
Spyridon Ntougias , Nektarios Kavroulakis , Kalliope K. Papadopoulou , Constantinos Ehaliotis , Georgios I. Zervakis
J. Microbiol. 2010;48(3):297-306.   Published online June 23, 2010
DOI: https://doi.org/10.1007/s12275-010-9193-y
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AbstractAbstract
Microbial assessment of grape marc wastes, the residual solid by-product of the wine-industry, was performed by identifying phylogenetically the fungal culturable diversity in order to evaluate environmental and disposal safety issues and to discuss ecological considerations of applications on agricultural land. Fungal spores in grape marc were estimated to 4.7×106 per g dry weight. Fifty six fungal isolates were classified into eight operational taxonomic units (OTUs) following amplified ribosomal DNA restriction analysis (ARDRA) and colony morphology. Based on 18S rRNA gene and 5.8S rRNA gene-ITS sequencing, the isolates representing OTUs #1, #2, #3, and #4, which comprised 44.6%, 26.8%, 12.5%, and 5.3%, respectively, of the number of the total isolates, were identified as Aspergillus fumigatus, Bionectria ochroleuca, Haematonectria haematococca, and Trichosporon mycotoxinivorans. The isolates of OTU#5 demonstrated high phylogenetic affinity with Penicillium spp., while members of OTUs #6 and #7 were closer linked with Geotrichum candidum var. citri-aurantii and Mycocladus corymbifer, respectively (95.4 and 97.9% similarities in respect to their 5.8S rRNA gene-ITS sequences). The OTU#8 with a single isolate was related with Aspergillus strains. It appears that most of the fungal isolates are associated with the initial raw material. Despite the fact that some of the species identified may potentially act as pathogens, measures such as the avoidance of maintaining large and unprocessed quantities of grape marc wastes in premises without adequate aeration, together with its suitable biological treatment (e.g., composting) prior to any agriculture-related application, could eliminate any pertinent health risks.
Research Support, Non-U.S. Gov'ts
Molecular Diversity of Chrysoviruses in Korean Isolates of A New Fungal Species, Cryphonectria nitschkei
Jung-Mi Kim , Jung-Ae Kim , Jin-Ah Park , Seung-Moon Park , Byeong-Jin Cha , Moon-Sik Yang , Dae-Hyuk Kim
J. Microbiol. 2009;47(4):441-447.   Published online September 9, 2009
DOI: https://doi.org/10.1007/s12275-009-0206-7
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AbstractAbstract
Genetic diversity of the chrysovirus within the four fungal strains was analyzed by comparing the full- length sequences of cloned chrysoviral genes encoding the RNA-dependent RNA polymerase (RdRp) and capsid protein (CP). Because the morphological characteristics of four chrysovirus-infected Cryphonectria spp. strains were different, strain identification was conducted via sequence comparison of the internal transcribed spacers (ITSs) of the fungal rRNA gene. Phylogenic analysis of the ITS regions revealed that the four strains were closely clustered with the reference strain of Cryphonectria nitschkei, while they were more distantly related to other common Cryphonectria species, indicating that they were likely C. nitschkei. Sequence comparison among chrysoviruses from Korean C. nitschkei strains revealed that similarities of the RdRp and CP genes ranged from 98% to 100% and from 95% to 100%, respectively, at the protein level. Their corresponding nucleotide sequences showed 97% to 100% and 84% to 100% identities, respectively. Compared to RdRp, the CP gene had more divergence, suggesting the presence of genes possessing different evolutionary rates within the chrysovirus genome. Sequence comparisons with other known chrysoviruses showed that the four Korean chrysoviruses were clustered together at the next lineage level. Discovering why two strains (bs131 and bs132) containing identical ITS sequences and chrysoviruses display different phenotypes should prove interesting.
A New Dactylella Species from Orbilia alba
ZeFen Yu , YanJie Kong , Ying Zhang , Min Qiao , JianWei Guo , Ke-Qin Zhang
J. Microbiol. 2009;47(3):265-269.   Published online June 26, 2009
DOI: https://doi.org/10.1007/s12275-008-0301-1
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AbstractAbstract
A new Dactylella species, Dactylella alba was isolated from the ascospores of Orbilia alba collected in Wen- shan County, Yunnan Province, China. Conidiophores were either not branched or occasionally branched, bearing divergent sterigmata on the tip with single conidium on each. Conidia were elongated ellipsoids, 1-2 septate, mostly 1 septate. By combining the ITS sequence with morphological characteristics, a new anamorphic species is described and illustrated together with its teleomorph.

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