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Research Support, Non-U.S. Gov't
- NOTE] Next-Generation Sequencing-Based Genome-Wide Mutation Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain
- Chang-Soo Lee , Jae-Young Nam , Eun-Suk Son , O-chul Kwon , Woorijarang Han , Jae-Yong Cho , Young-Jin Park
- J. Microbiol. 2012;50(5):860-863. Published online November 4, 2012
- DOI: https://doi.org/10.1007/s12275-012-2109-2
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- 9 Crossref
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Abstract
- In order to identify single nucleotide polymorphism and insertion/deletion mutations, we performed whole-genome re-sequencing of the enhanced L-lysine-producing Corynebacterium glutamicum ATCC 21300 strain. In total, 142 single nucleotide polymorphisms and 477 insertion/deletion mutations were identified in the ATCC 21300 strain when compared to 3,434 predicted genes of the wild-type C. glutamicum ATCC 13032 strain. Among them, 110 transitions and 29 transversions of single nucleotide polymorphisms were found from genes of the ATCC 21300 strain. In addition, 11 genes, involved in the L-lysine biosynthetic pathway and central carbohydrate metabolism, contained mutations including single nucleotide polymorphisms and insertions/deletions. Interestingly, RT-PCR analysis of these 11 genes indicated that they were normally expressed in the ATCC 21300 strain. This information of genome-wide gene-associated variations will be useful for genome breeding of C. glutamicum in order to develop an industrial amino acidproducing strain with minimal mutation.
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Citations
Citations to this article as recorded by
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Biotechnology Advances.2015; 33(8): 1653. CrossRef - Characterization of a Corynebacterium glutamicum dnaB mutant that shows temperature-sensitive growth and mini-cell formation
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Archives of Microbiology.2014; 196(12): 871. CrossRef - Next-generation sequencing-based transcriptome analysis of l-lysine-producing Corynebacterium glutamicum ATCC 21300 strain
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Journal of Microbiology.2013; 51(6): 877. CrossRef
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