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- Structural basis for the ATP-independent proteolytic activity of LonB proteases and reclassification of their AAA+ modules
- Young Jun An , Jung-Hyun Na , Myung-Il Kim , Sun-Shin Cha
- J. Microbiol. 2015;53(10):711-717. Published online October 2, 2015
- DOI: https://doi.org/10.1007/s12275-015-5417-5
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Abstract
- Lon proteases degrade defective or denature proteins as well as some folded proteins for the control of cellular protein quality. There are two types of Lon proteases, LonA and LonB. Each consists of two functional components: a protease component and an ATPase associated with various cellular activities (AAA+ module). Here, we report the 2.03 Å-resolution crystal structure of the isolated AAA+ module (iAAA+ module) of LonB from Thermococcus onnurineus NA1 (TonLonB). The iAAA+ module, having no bound nucleotide, adopts a conformation virtually identical to the ADP-bound conformation of AAA+ modules in the hexameric structure of TonLonB; this provides insights into the ATP-independent proteolytic activity observed in a LonB protease. Structural comparison of AAA+ modules between LonA and LonB revealed that the AAA+ modules of Lon proteases are separated into two distinct clades depending on their structural features. The AAA+ module of LonB belongs to the ‘H2 & Ins1 insert clade (HINS clade)’ defined for the first time in this study, while the AAA+ module of LonA is a member of the HCLR clade.
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- Unique Structural Fold of LonBA Protease from Bacillus subtilis, a Member of a Newly Identified Subfamily of Lon Proteases
Alla Gustchina, Mi Li, Anna G. Andrianova, Arsen M. Kudzhaev, George T. Lountos, Bartosz Sekula, Scott Cherry, Joseph E. Tropea, Ivan V. Smirnov, Alexander Wlodawer, Tatyana V. Rotanova
International Journal of Molecular Sciences.2022; 23(19): 11425. CrossRef - Structure and the Mode of Activity of Lon Proteases from Diverse Organisms
Alexander Wlodawer, Bartosz Sekula, Alla Gustchina, Tatyana V. Rotanova
Journal of Molecular Biology.2022; 434(7): 167504. CrossRef - Proteolytic systems of archaea: slicing, dicing, and mincing in the extreme
Nicholas P. Robinson, Julie A. Maupin-Furlow
Emerging Topics in Life Sciences.2018; 2(4): 561. CrossRef
- Unique Structural Fold of LonBA Protease from Bacillus subtilis, a Member of a Newly Identified Subfamily of Lon Proteases
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