MSK
- About
- Browse Articles
-
Special Issues
- Pioneering strategies for overcoming bacterial drug resistance (2026)
- Advancing microbial engineering through synthetic biology (2025)
- Host-associated microbiome (2024)
- Bacterial regulatory mechanisms for the control of complex cellular mechanisms (2023)
- Two years into COVID-19 pandemic: Where are we? (2022)
- Collections
- For Contributors
- Policies
- E-Submission
- About
- Browse Articles
-
Special Issues
- Pioneering strategies for overcoming bacterial drug resistance (2026)
- Advancing microbial engineering through synthetic biology (2025)
- Host-associated microbiome (2024)
- Bacterial regulatory mechanisms for the control of complex cellular mechanisms (2023)
- Two years into COVID-19 pandemic: Where are we? (2022)
- Collections
- Policies
- For Contributors
Search
- Page Path
- HOME > Search
Research article
- Berberine promotes host lipolysis to enhance antimicrobial defense against hypervirulent Klebsiella pneumoniae infection
- Ju Yeong Lee, Hui-Jung Jung, Anwesha Ash, Seunghyeon Jeon, Miri Hyun, Ji Yeon Lee, Sang-Hee Lee, Hyuk Nam Kwon, Won-Ki Baek, Jichan Jang, Hyun ah Kim, Jin Kyung Kim
- J. Microbiol. 2026;64(8):e2605001. Published online August 31, 2026
- DOI: https://doi.org/10.71150/jm.2605001
- 150 View
- 6 Download
-
Abstract
PDF
Supplementary Material -
Hypervirulent Klebsiella pneumoniae (hvKp) is an emerging pathogen that causes severe community-acquired infections; however, the immune mechanisms controlling intracellular hvKp have yet to be clearly defined. In this study, we investigated the therapeutic potential of berberine against hvKp infection and elucidated the underlying molecular mechanisms using macrophage cell models and in vivo zebrafish models. Berberine significantly reduced intracellular hvKp survival in macrophages and improved survival in hvKp-infected zebrafish. Berberine markedly attenuated proinflammatory cytokine production and inhibited the c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) signaling pathways. Notably, we found that hvKp exploited host lipid droplets (LD) biosynthesis to support its intracellular survival, and berberine effectively suppressed LD accumulation. Mechanistically, berberine promoted the nuclear translocation of transcription factor EB (TFEB), thereby enhancing lipolysis. Although berberine upregulated autophagy-related gene expression during hvKp infection, it did not induce lipophagy, the selective autophagic degradation of LD. Collectively, these findings indicate that berberine has potential as a therapeutic agent against hvKp infection by modulating host lipid metabolism to restrict bacterial intracellular survival.
Article
- Performance comparison of fecal preservative and stock solutions for gut microbiome storage at room temperature
- Chanhyeok Park , Kyeong Eui Yun , Jeong Min Chu , Ji Yeon Lee , Chang Pyo Hong , Young Do Nam , Jinuk Jeong , Kyudong Han , Yong Ju Ahn
- J. Microbiol. 2020;58(8):703-710. Published online June 25, 2020
- DOI: https://doi.org/10.1007/s12275-020-0092-6
- 1,031 View
- 10 Download
- 16 Web of Science
- 17 Crossref
-
Abstract
PDF
-
The gut microbiome, which is symbiotic within the human
body, assists in human digestion. It plays significant roles
in identifying intestinal disease as well as in maintaining a
healthy body with functional immune and metabolic activities.
To confirm the consistency of fecal intestinal microbial
research, it is necessary to study the changes in intestinal microbial
flora according to the fecal collection solution and
storage period. We collected fecal samples from three healthy
Korean adults. To examine the efficacy of fecal collection solution,
we used NBgene-Gut, OMNIgene-Gut, 70% ethanol
(Ethanol-70%), and RNAlater. The samples were stored for
up to two months at room temperature using three different
methods
, and we observed changes in microbial communities over time. We analyzed clusters of changes in the microbial flora by observing fecal stock solutions and metagenome sequencing performed over time. In particular, we confirmed the profiling of alpha and beta diversity and microbial classification according to the differences in intestinal environment among individuals. We also confirmed that the microbial profile remained stable for two months and that the microbial profile did not change significantly over time. In addition, our results suggest the possibility of verifying microbial profiling even for long-term storage of a single sample. In conclusion, collecting fecal samples using a stock solution rather than freezing feces seems to be relatively reproducible and stable for GUT metagenome analysis. Therefore, stock solution tubes in intestinal microbial research can be used without problems. -
Citations
Citations to this article as recorded by
- Five-Day Treatment with B. licheniformis Along with Classical Vancomycin Treatment Was Effective in Preserving Gut Microbiota in Patients with Clostridioides difficile Infection
Tae-Geun Gweon, Sang-Bum Kang, Soo-Young Na, Dong Jun Oh, Sang Wook Kim, Geom Seog Seo, Joo Young Cho
Nutrients.2025; 17(4): 641. CrossRef - Stabilized and unstabilized sampling methods result in differential fecal 16S rRNA microbial sequencing results
Christopher E. Stamper, Andrew J. Hoisington, Joseph C. Ellis, Christopher A. Lowry, Lisa A. Brenner, Theodore Raymond Muth
PLOS One.2025; 20(8): e0324351. CrossRef - Stability of oral and fecal microbiome at room temperature: impact on diversity
Blanca Rius-Sansalvador, David Bars-Cortina, Olfat Khannous-Lleiffe, Ainhoa Garcia-Serrano, Elisabet Guinó, Ester Saus, Toni Gabaldón, Victor Moreno, Mireia Obón-Santacana
Frontiers in Microbiomes.2025;[Epub] CrossRef - Omnigene-Guttm ensures fecal microbiome stability in the pediatric population
Raoull Hoogendijk, Thijs J. M. van den Broek, Hyunju Lee, Sabine Mueller, Cassie Kline, John Bianco, Janetta Top, Marcel R. de Zoete, Lennart Kester, Friso Calkoen, Jasper van der Lugt
AMB Express.2024;[Epub] CrossRef - Microbes for lung cancer detection: feasibility and limitations
Sirui Zhou, Weijian Zhu, Hehua Guo, Yalan Nie, Jiazheng Sun, Ping Liu, Yulan Zeng
Frontiers in Oncology.2024;[Epub] CrossRef - Importance of Human Faecal Biobanking: From Collection to Storage
Izabela Lewandowska, Katarzyna Grzech, Jolanta Krzysztoń-Russjan
Advancements of Microbiology.2024; 63(4): 181. CrossRef - Effects of Stool Sample Preservation Methods on Gut Microbiota Biodiversity: New Original Data and Systematic Review with Meta-Analysis
Xin-meng Li, Xiao Shi, Yao Yao, Yi-cun Shen, Xiang-ling Wu, Ting Cai, Lun-xi Liang, Fen Wang, Po-Yu Liu
Microbiology Spectrum.2023;[Epub] CrossRef - Microbial Composition and Stool Short Chain Fatty Acid Levels in Fibromyalgia
Yunkyung Kim, Geun-Tae Kim, Jihun Kang
International Journal of Environmental Research and Public Health.2023; 20(4): 3183. CrossRef - Best practice for wildlife gut microbiome research: A comprehensive review of methodology for 16S rRNA gene investigations
Leigh Combrink, Ian R. Humphreys, Quinn Washburn, Holly K. Arnold, Keaton Stagaman, Kristin D. Kasschau, Anna E. Jolles, Brianna R. Beechler, Thomas J. Sharpton
Frontiers in Microbiology.2023;[Epub] CrossRef - Effects of Waterlogging on Soybean Rhizosphere Bacterial Community Using V4, LoopSeq, and PacBio 16S rRNA Sequence
Taobing Yu, Lang Cheng, Qi Liu, Shasha Wang, Yuan Zhou, Hongbin Zhong, Meifang Tang, Hai Nian, Tengxiang Lian, Cheng Gao, Glade Dlott
Microbiology Spectrum.2022;[Epub] CrossRef - Effects of Stool Sample Preservation Methods on Gut Microbiota Biodiversity: New Original Data and Systematic Review with Meta-Analysis
Xin-meng Li, Xiao Shi, Yao Yao, Yi-cun Shen, Xiang-ling Wu, Fen Wang
SSRN Electronic Journal .2022;[Epub] CrossRef - Trialling a microbiome-targeted dietary intervention in children with ADHD—the rationale and a non-randomised feasibility study
Kate Lawrence, Kyriaki Myrissa, Miguel Toribio-Mateas, Lori Minini, Alice M. Gregory
Pilot and Feasibility Studies.2022;[Epub] CrossRef - Can the genetic variability of Blastocystis sp. be associated with the climatic region of its human carriers?
B. Ake-Canche, E. Rodriguez-Bataz, J.Y Esquivel-Piña, A. Tolentino-Loreto, S. Arroyo-Escalante, J. Martínez-Ocaña, M. Romero-Valdovinos, O. Valenzuela, G.E. Orozco-Mosqueda, F. Martinez-Hernandez, P. Maravilla, A. Martinez
Infection, Genetics and Evolution.2022; 106: 105383. CrossRef - Critical evaluation of faecal microbiome preservation using metagenomic analysis
Alena L Pribyl, Donovan H Parks, Nicola Z Angel, Joel A Boyd, Alexander G Hasson, Liang Fang, Samantha L MacDonald, Blake A Wills, David L A Wood, Lutz Krause, Gene W Tyson, Philip Hugenholtz
ISME Communications.2021;[Epub] CrossRef - The effect of taxonomic classification by full-length 16S rRNA sequencing with a synthetic long-read technology
Jinuk Jeong, Kyeongeui Yun, Seyoung Mun, Won-Hyong Chung, Song-Yi Choi, Young-do Nam, Mi Young Lim, Chang Pyo Hong, ChanHyeok Park, Yong Ju Ahn, Kyudong Han
Scientific Reports.2021;[Epub] CrossRef - Dietary fiber and probiotics influence the gut microbiome and melanoma immunotherapy response
Christine N. Spencer, Jennifer L. McQuade, Vancheswaran Gopalakrishnan, John A. McCulloch, Marie Vetizou, Alexandria P. Cogdill, Md A. Wadud Khan, Xiaotao Zhang, Michael G. White, Christine B. Peterson, Matthew C. Wong, Golnaz Morad, Theresa Rodgers, Jona
Science.2021; 374(6575): 1632. CrossRef - Impact of Plant-Based Meat Alternatives on the Gut Microbiota of Consumers: A Real-World Study
Miguel A. Toribio-Mateas, Adri Bester, Natalia Klimenko
Foods.2021; 10(9): 2040. CrossRef
- Five-Day Treatment with B. licheniformis Along with Classical Vancomycin Treatment Was Effective in Preserving Gut Microbiota in Patients with Clostridioides difficile Infection
First
Prev
TOP
