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Research Support, Non-U.S. Gov't
- Note] Identification of High-Specificity H-NS Binding Site in LEE5 Promoter of Enteropathogenic Esherichia coli (EPEC)
- Abhay Prasad Bhat , Minsang Shin , Hyon E. Choy
- J. Microbiol. 2014;52(7):626-629. Published online March 7, 2014
- DOI: https://doi.org/10.1007/s12275-014-3562-x
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Abstract
- Histone-like nucleoid structuring protein (H-NS) is a small but abundant protein present in enteric bacteria and is involved in compaction of the DNA and regulation of the transcription. Recent reports have suggested that H-NS binds to a specific AT rich DNA sequence than to intrinsically curved DNA in sequence independent manner. We detected two high-specificity H-NS binding sites in LEE5 promoter of EPEC centered at -110 and -138, which were close to the proposed consensus H-NS binding motif. To identify H-NS binding sequence in LEE5 promoter, we took a random mutagenesis approach and found the mutations at around -138 were specifically defective in the regulation byH-NS. It was concluded that H-NS exertsmaximumrepression via the specific sequence at around -138 and ubsequently contacts α subunit of RNAP through oligomerization.
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Citations
Citations to this article as recorded by
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Journal of Bacteriology.2018;[Epub] CrossRef -
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Journal of Microbiology.2015; 53(4): 250. CrossRef
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