Probiotics such as lactobacilli and bifidobacteria have healthpromoting
effects by immune modulation. In the present
study, we examined the immunomodulatory properties of
Lactobacillus curvatus WiKim38, which was newly isolated
from baechu (Chinese cabbage) kimchi. The ability of L.
curvatus WiKim38 to induce cytokine production in bone
marrow-derived dendritic cells (BMDCs) was determined by
enzyme-linked immunosorbent assay. To evaluate the molecular
mechanisms underlying L. curvatus Wikim38-mediated
IL-10 production, Western blot analyses and inhibitor
assays were performed. Moreover, the in vivo anti-inflammatory
effects of L. curvatus WiKim38 were examined in a dextran
sodium sulfate (DSS)-induced colitis mouse model. L.
curvatus WiKim38 induced significantly higher levels of IL-
10 in BMDCs compared with that induced by LPS. NF-κB
and ERK were activated by L. curvatus WiKim38, and an
inhibitor assay revealed that these pathways were required
for L. curvatus WiKim38-induced production of IL-10 in
BMDCs. An in vivo experiment showed that oral administration
of L. curvatus WiKim38 increased the survival rate
of mice with DSS-induced colitis and improved clinical signs
and histopathological severity in colon tissues. Taken together,
these results indicate that L. curvatus Wikim38 may
have health-promoting effects via immune modulation, and
may thus be applicable for therapy of various inflammatory
diseases.
Citations
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