Journal of Microbiology

Journal Article

NOTE] Leucobacter denitrificans sp. nov., Isolated from Cow Dung: Hang-Yeon Weon , Rangasamy Anandham , Tomohiko Tamura , Moriyuki Hamada , Soo-Jin Kim , Yi-Seul Kim , Ken-ichiro Suzuki , Soon-Wo Kwon; J. Microbiol. 2012;50(1):161-165. Published online February 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1324-1

Abstract: The bacterial strain M1T8B10T was isolated from cow dung in Suwon, Republic of Korea. The strain was a Gram stainpositive rod, nonmotile, and non-spore-forming. According to 16S rRNA gene sequence analysis, the strain fell within the clade of the genus Leucobacter, showing the highest sequence similarities with Leucobacter aridicollis L-9T (98.7%), Leucobacter iarius 40T (98.4%), and Leucobacter komagatae JCM 9414T (98.2%). Cell-wall peptidoglycan contained the diagnostic diamino acid 2,4-diaminobutyric acid of the genus Leucobacter, showing B-type cross-linked peptidoglycans. The major fatty acids were anteiso-C15:0, iso-C16:0, and anteiso- C17:0. The quinone system consisted of the menaquinones MK-11 (78%) and MK-10 (22%). The polar lipid profiles contained diphosphatidylglycerol, phosphatidylglycerol, and an unidentified glycolipid. Differences in several physiological features including nitrate reduction enabled the isolate to be differentiated from all recognized Leucobacter species. Based on these phylogenetic, chemotaxonomic, and phenotypic results, the isolate represents a novel species, for which the name Leucobacter denitrificans sp. nov. is proposed. The type strain is M1T8B10T (=KACC 14055T =NBRC 106309T).

Research Support, Non-U.S. Gov't

NOTE] Leucobacter kyeonggiensis sp. nov., a New Species Isolated from Dye Waste Water: Hyun-jung Kim , Sang-Seob Lee; J. Microbiol. 2011;49(6):1044-1049. Published online December 28, 2011; DOI: https://doi.org/10.1007/s12275-011-1548-5

Abstract: A Gram-positive, aerobic, non-motile bacterium designated F3-P9T, was isolated from dye waste water in Korea and was characterized using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain F3-P9T belongs to genus Leucobacter. The 16S rRNA gene sequence similarities
among strain F3-P9T and validated representatives of the genus Leucobacter ranged from 95.9-97.4%. Strain F3-P9T exhibited DNA-DNA relatedness values below 48% with respect to Leucobacter species. The G+C content of the genomic DNA was 67.5 mol%. F3-P9T contained MK-11 as the major respiratory quinone. The major fatty acids were anteiso-C15:0 (48.5%), anteiso-C17:0 (22.7%), and iso-C16:0 (14.5%). The peptidoglycan was composed of L-2,4-diaminbutyric acid, alanine, glycine, and glutamic acid. The polar lipid profile showed a major amount of diphosphatidylglycerol (DPG), a moderate amount of phosphatidylglycerol (PG), and two unknown glycolipids. On the basis of its phenotypic and genotypic properties and its phylogenetic distinctiveness, strain F3-P9T (KEMC 211-128T =KACC 16572T =JCM 17539T) should be classified in the genus Leucobacter as the type strain of a novel species, for which the name Leucobacter kyeonggiensis sp. nov. is proposed.

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