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- Lactobacillus gasseri BNR17 and Limosilactobacillus fermentum ABF21069 Ameliorate High Sucrose-Induced Obesity and Fatty Liver via Exopolysaccharide Production and β-oxidation
- Yu Mi Jo, Yoon Ji Son, Seul-Ah Kim, Gyu Min Lee, Chang Won Ahn, Han-Oh Park, Ji-Hyun Yun
- J. Microbiol. 2024;62(10):907-918. Published online October 17, 2024
- DOI: https://doi.org/10.1007/s12275-024-00173-6
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Abstract
- Obesity and metabolic dysfunction-associated fatty liver disease (MAFLD) are prevalent metabolic disorders with substantial global health implications that are often inadequately addressed by current treatments and may have side effects. Probiotics have emerged as promising therapeutic agents owing to their beneficial effects on gut health and metabolism. This study investigated the synergistic effects of a probiotic combination of BNR17 and ABF21069 on obesity and MAFLD in C57BL/6 mice fed a high-sucrose diet. The probiotic combination significantly reduced body weight and fat accumulation compared with the high-sucrose diet. It also alleviated elevated serum leptin levels induced by a high-sucrose diet. Histological analysis revealed a significant reduction in white adipose tissue and fatty liver in the mice treated with the probiotic combination. Furthermore, increased expression of genes related to β-oxidation, thermogenesis, and lipolysis suggested enhanced metabolic activity. The probiotic groups, particularly the BNR17 group, showed an increase in fecal exopolysaccharides, along with a tendency toward a lower expression of intestinal sugar transport genes, indicating reduced sugar absorption. Additionally, inflammatory markers in the liver tissue exhibited lower expression in the ABF21069 group than in the HSD group. Despite each strain in the combination group having distinct characteristics and functions, their combined effect demonstrated synergy in mitigating obesity and MAFLD, likely through the modulation of fecal exopolysaccharides content and improvement in lipid metabolism. These findings underscore the potential of probiotic supplementation as a promising assistant therapy for managing obesity and MAFLD and provide valuable insights into its therapeutic mechanisms in metabolic disorders.
- The protein and neutral lipid composition of lipid droplets isolated from the fission yeast, Schizosaccharomyces pombe
- Alex Meyers , Karuna Chourey , Taylor M. Weiskittel , Susan Pfiffner , John R. Dunlap , Robert L. Hettich , Paul Dalhaimer
- J. Microbiol. 2017;55(2):112-122. Published online January 26, 2017
- DOI: https://doi.org/10.1007/s12275-017-6205-1
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- 16 Crossref
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Abstract
- Lipid droplets consist of a core of neutral lipids surrounded by a phospholipid monolayer with bound proteins. Much of the information on lipid droplet function comes from proteomic and lipodomic studies that identify the components of droplets isolated from organisms throughout the phylogenetic tree. Here, we add to that important inventory by reporting lipid droplet factors from the fission yeast, Schizosaccharomyces pombe. Unique to this study was the fact that cells were cultured in three different environments: 1) late log growth phase in glucose-based media, 2) stationary phase in glucosebased media, and 3) late log growth phase in media containing oleic acid. We confirmed colocalization of major factors with lipid droplets using live-cell fluorescent microscopy. We also analyzed droplets from each of the three conditions for sterol ester (SE) and triacylglycerol (TAG) content, along with their respective fatty acid compositions. We identified a previously undiscovered lipid droplet protein, Vip1p, which affects droplet size distribution. The results provide further insight into the workings of these ubiquitous organelles.
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- Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast
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PLOS Genetics.2024; 20(12): e1011509. CrossRef - Mild Heat Stress Alters the Physical State and Structure of Membranes in Triacylglycerol-Deficient Fission Yeast, Schizosaccharomyces pombe
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Cell Death Discovery.2023;[Epub] CrossRef - Oleaginous yeasts: Time to rethink the definition?
José Manuel Salvador López, Meriam Vandeputte, Inge N. A. Van Bogaert
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Molecular Biology of the Cell.2022;[Epub] CrossRef - Lipid Droplet Nucleation
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Trends in Cell Biology.2021; 31(2): 108. CrossRef - Metabolism of Storage Lipids and the Role of Lipid Droplets in the Yeast Schizosaccharomyces pombe
Ivan Hapala, Peter Griac, Roman Holic
Lipids.2020; 55(5): 513. CrossRef - Lipid Droplets in Neurodegenerative Disorders
Brandon C. Farmer, Adeline E. Walsh, Jude C. Kluemper, Lance A. Johnson
Frontiers in Neuroscience.2020;[Epub] CrossRef - Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts
Neha Arora, Hong-Wei Yen, George P. Philippidis
Sustainability.2020; 12(12): 5125. CrossRef - Mechanisms of protein targeting to lipid droplets: A unified cell biological and biophysical perspective
Ravi Dhiman, Stefanie Caesar, Abdou Rachid Thiam, Bianca Schrul
Seminars in Cell & Developmental Biology.2020; 108: 4. CrossRef - The New Face of the Lipid Droplet: Lipid Droplet Proteins
Congyan Zhang, Pingsheng Liu
PROTEOMICS.2019;[Epub] CrossRef - Effect of Selenium on Lipid and Amino Acid Metabolism in Yeast Cells
Marek Kieliszek, Stanisław Błażejak, Anna Bzducha-Wróbel, Anna M. Kot
Biological Trace Element Research.2019; 187(1): 316. CrossRef - The Peroxygenase Activity of the Aspergillus flavus Caleosin, AfPXG, Modulates the Biosynthesis of Aflatoxins and Their Trafficking and Extracellular Secretion via Lipid Droplets
Abdulsamie Hanano, Mari Alkara, Ibrahem Almousally, Mouhnad Shaban, Farzana Rahman, Mehedi Hassan, Denis J. Murphy
Frontiers in Microbiology.2018;[Epub] CrossRef - Mitotic defects in fission yeast lipid metabolism ‘cut’ mutants are suppressed by ammonium chloride
Róbert Zach, Jarmila Tvarůžková, Martin Schätz, Ondřej Ťupa, Beáta Grallert, Martin Převorovský
FEMS Yeast Research.2018;[Epub] CrossRef - Lipid Droplets: Formation to Breakdown
Alex Meyers, Taylor M. Weiskittel, Paul Dalhaimer
Lipids.2017; 52(6): 465. CrossRef
- Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast
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