The microbiota of lactic acid bacteria (LAB) in thirty-five
samples of Miang, a traditional fermented tea leaf product,
collected from twenty-two different regions of eight provinces
in upper northern Thailand was revealed through the
culture-dependent technique. A total of 311 presumptive
LAB strains were isolated and subjected to clustering analysis
based on repetitive genomic element-PCR (rep-PCR) fingerprinting
profiles. The majority of the strains belonged to
the Lactobacillus genera with an overwhelming predominance
of the Lb. plantarum group. Further studies of species-specific
PCR showed that 201 of 252 isolates in the Lb. plantarum
group were Lb. plantarum which were thus considered
as the predominant LAB in Miang, while the other 51 isolates
belonged to Lb. pentosus. In contrast to Lb. plantarum,
there is a lack of information on the tannase gene and the
tea tannin-tolerant ability of Lb. pentosus. Of the 51 Lb. pentosus
isolates, 33 were found to harbor the genes encoding
tannase and shared 93-99% amino acid identity with tannase
obtained from Lb. pentosus ATCC 8041T. Among 33
tannase gene-positive isolates, 23 isolates exhibited high tannin-
tolerant capabilities when cultivated on de Man Rogosa
and Sharpe agar-containing bromocresol purple (0.02 g/L,
MRS-BCP) supplemented with 20% (v/v) crude tea extract,
which corresponded to 2.5% (w/v) tannins. These Lb. pentosus
isolates with high tannin-tolerant capacity are expected
to be the high potential strains for functional tannase production involved in Miang fermentation as they will bring
about certain benefits and could be used to improve the
fermentation of tea products.
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