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- CA‑CAS‑01‑A: A Permissive Cell Line for Isolation and Live Attenuated Vaccine Development Against African Swine Fever Virus
- Seung-Chul Lee , Yongkwan Kim , Ji-Won Cha , Kiramage Chathuranga , Niranjan Dodantenna , Hyeok-Il Kwon , Min Ho Kim , Weonhwa Jheong , In-Joong Yoon , Joo Young Lee , Sung-Sik Yoo , Jong-Soo Lee
- J. Microbiol. 2024;62(2):125-134. Published online March 13, 2024
- DOI: https://doi.org/10.1007/s12275-024-00116-1
- 336 View
- 4 Download
- 7 Web of Science
- 4 Crossref
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Abstract
PDF - African swine fever virus (ASFV) is the causative agent of the highly lethal African swine fever disease that affects domestic pigs and wild boars. In spite of the rapid spread of the virus worldwide, there is no licensed vaccine available. The lack of a suitable cell line for ASFV propagation hinders the development of a safe and effective vaccine. For ASFV propagation, primary swine macrophages and monocytes have been widely studied. However, obtaining these cells can be time-consuming and expensive, making them unsuitable for mass vaccine production. The goal of this study was to validate the suitability of novel CA-CAS-01-A (CAS-01) cells, which was identified as a highly permissive cell clone for ASFV replication in the MA-104 parental cell line for live attenuated vaccine development. Through a screening experiment, maximum ASFV replication was observed in the CAS-01 cell compared to other sub-clones of MA-104 with 14.89 and log10 7.5 ± 0.15 Ct value and TCID50/ ml value respectively. When CAS-01 cells are inoculated with ASFV, replication of ASFV was confirmed by Ct value for ASFV DNA, HAD50/ ml assay, TCID50/ ml assay, and cytopathic effects and hemadsoption were observed similar to those in primary porcine alveolar macrophages after 5th passage. Additionally, we demonstrated stable replication and adaptation of ASFV over the serial passage. These results suggest that CAS-01 cells will be a valuable and promising cell line for ASFV isolation, replication, and development of live attenuated vaccines.
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Citations
Citations to this article as recorded by
- Establishment of a highly sensitive porcine alveolar macrophage cell line for African swine fever virus
Xiangwan Lu, Xiadan Gong, Yingshuo Sun, Lang Gong, Yan Zhang
In Vitro Cellular & Developmental Biology - Animal.2025; 61(4): 425. CrossRef - Genetic and Pathogenic Characteristic of High Pathogenic Korean NADC34‐Like Porcine Reproductive and Respiratory Syndrome Virus
Sehyeong Ham, Chanhee Chae, Nan-hua Chen
Transboundary and Emerging Diseases.2025;[Epub] CrossRef - Efficient and modular reverse genetics system for rapid generation of recombinant severe acute respiratory syndrome coronavirus 2
Sojung Bae, Jinjong Myoung
Journal of Microbiology.2025; 63(7): e2504015. CrossRef - Development and characterization of high-efficiency cell-adapted live attenuated vaccine candidate against African swine fever
Min Ho Kim, Ashan Subasinghe, Yongkwan Kim, Hyeok-Il Kwon, Yehjin Cho, Kiramage Chathuranga, Ji-Won Cha, Ji-Yoon Moon, Ji-Hyeon Hong, Jin Kim, Seung-Chul Lee, Niranjan Dodantenna, Nuwan Gamage, W. A. Gayan Chathuranga, Yeonji Kim, In-Joong Yoon, Joo Young
Emerging Microbes & Infections.2024;[Epub] CrossRef
- Establishment of a highly sensitive porcine alveolar macrophage cell line for African swine fever virus
Research Support, Non-U.S. Gov't
- NOTE] Microbacterium suwonense sp. nov., Isolated from Cow Dung
- Rangasamy Anandham , Tomohiko Tamura , Moriyuki Hamada , Hang-Yeon Weon , Soo-Jin Kim , Yi-Seul Kim , Ken-ichiro Suzuki , Soon-Wo Kwon
- J. Microbiol. 2011;49(5):852-856. Published online November 9, 2011
- DOI: https://doi.org/10.1007/s12275-011-1036-y
- 159 View
- 0 Download
- 11 Crossref
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Abstract
PDF
- An actinomycete strain, designated M1T8B9T, was isolated from cow dung in Suwon, Republic of Korea. The isolate was a Gram-positive, nonmotile, and non-spore-forming bacterium. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the genus Microbacterium, with its closest neighbors being Microbacterium soli DCY17T (98.2%) and Microbacterium esteraromaticum DSM 8609T (98.0%). The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, and one unknown glycolipid. Strain M1T8B9T contained the major fatty acids C15:0 anteiso, C16:0 iso, C17:0 anteiso, and C15:0 iso, and the cell-wall peptidoglycan was of type B2β. According to DNA-DNA hybridization studies, strain M1T8B9T showed 42% and 26% relatedness with M. soli DCY17T and M. esteraromaticum DSM 8609T, respectively. On the basis of the data presented, strain M1T8B9T is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium suwonense sp. nov. is proposed. The type strain is M1T8B9T (=KACC 14058T =NBRC 106310T).
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Citations
Citations to this article as recorded by- Plant growth-promoting bacteria from dung of indigenous and exotic cow breeds and their effect on the growth of pea plant in sustainable agriculture
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Biotechnology for the Environment.2025;[Epub] CrossRef - The Isolation and Characterization of a Novel Psychrotolerant Cellulolytic Bacterium, Microbacterium sp. QXD-8T
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Microorganisms.2024; 12(2): 303. CrossRef - Harnessing the Power of Traditional Organic Formulations for Crop Growth and Microbial Harmony
Errakutty Arunan Yuvasri, Rangasamy Anandham, Dananjeyan Balachandar, Murugaiyan Senthilkumar, Subramaniam Thiyageshwari, Saminathan Vincent
Frontiers in Bioscience-Elite.2024;[Epub] CrossRef -
Microbacterium tenebrionis sp. nov. and Microbacterium allomyrinae sp. nov., isolated from larvae of Tenebrio molitor L. and Allomyrina dichotoma, respectively
Soon Dong Lee, In Seop Kim
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef - Four new Microbacterium species isolated from seaweeds and reclassification of five Microbacterium species with a proposal of Paramicrobacterium gen. nov. under a genome-based framework of the genus Microbacterium
Soon Dong Lee, Hong Lim Yang, In Seop Kim
Frontiers in Microbiology.2023;[Epub] CrossRef -
Microbacterium protaetiae sp. nov., isolated from gut of larva of Protaetia brevitarsis seulensis
Jun Heo, Hayoung Cho, Mi Ae Kim, Moriyuki Hamada, Tomohiko Tamura, Satomi Saitou, Soo-Jin Kim Kim, Soon-Wo Kwon
International Journal of Systematic and Evolutionary Microbiology .2020; 70(4): 2226. CrossRef -
Microbacterium wangchenii sp. nov., isolated from faeces of Tibetan gazelles (Procapra picticaudata) on the Qinghai-Tibet Plateau
Kui Dong, Jing Yang, Shan Lu, Ji Pu, Xin-He Lai, Dong Jin, Junqin Li, Gui Zhang, Xiaoxia Wang, Sihui Zhang, Wenjing Lei, Zhihong Ren, Xiaomin Wu, Ying Huang, Suping Wang, Jianguo Xu
International Journal of Systematic and Evolutionary Microbiology .2020; 70(2): 1307. CrossRef -
Azospirillum ramasamyi sp. nov., a novel diazotrophic bacterium isolated from fermented bovine products
Rangasamy Anandham, Jun Heo, Ramasamy Krishnamoorthy, Murugaiyan SenthilKumar, Nellaiappan Olgaganathan Gopal, Soo-Jin Kim, Soon-Wo Kwon
International Journal of Systematic and Evolutionary Microbiology .2019; 69(5): 1369. CrossRef - Cultivable bacterial diversity and early plant growth promotion by the traditional organic formulations prepared using organic waste materials
Rangasamy Anandham, Nagaiah Premalatha, Hyeong Jin Jee, Hang Yeon Weon, Soon Wo Kwon, Ramasamy Krishnamoorthy, Pandiyan Indira Gandhi, Yong Ki Kim, Nellaiappan Olaganathan Gopal
International Journal of Recycling of Organic Waste in Agriculture.2015; 4(4): 279. CrossRef - Biodegradation of oxyethylated fatty alcohols by bacteria Microbacterium strain E19
Dorota Nowicka, Dobrochna Ginter-Kramarczyk, Aleksandra Holderna-Odachowska, Irena Budnik, Ewa Kaczorek, Zenon Lukaszewski
Ecotoxicology and Environmental Safety.2013; 91: 32. CrossRef - List of new names and new combinations previously effectively, but not validly, published
International Journal of Systematic and Evolutionary Microbiology .2012; 62(Pt_3): 473. CrossRef
- Plant growth-promoting bacteria from dung of indigenous and exotic cow breeds and their effect on the growth of pea plant in sustainable agriculture
Journal Article
- Symbiotic Relationship between Microbacterium sp. SK0812 and Candida tropicalis SK090404
- Seung Won Kang , Bo Young Jeon , Tae Sik Hwang , Doo Hyun Park
- J. Microbiol. 2009;47(6):721-727. Published online February 4, 2010
- DOI: https://doi.org/10.1007/s12275-009-0146-2
- 174 View
- 0 Download
- 4 Crossref
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Abstract
PDF
- A bacterium growing inside yeast cytoplasm was observed by light microscope without staining. The bacterium was separately stained from yeast cell by a fluorescent dye, 4′,6-diamidino-2-phenylindole (DAPI). The bacterium actively moved inside yeast cytoplasm and propagated in company with the yeast growth. The bacterium was separated from the yeast cytoplasm by selective disruption of yeast cells and the yeast without the intracellular bacterium (YWOB) was obtained by selective inactivation of bacterial cells. The yeast and the intracellular bacterium were identified as Candida tropicalis and Microbacterium sp., respectively. The length of Microbacterium sp. and C. tropicalis measured with SEM image was smaller than 0.5 μm and was larger than 5 μm, respectively. The yeast with the intracellular bacterium (YWIB) grew in a starch-based medium but the YWOB was not C. tropicalis has neither extracellular nor intracellular saccharification enzyme. Glucose was produced from starch by the extracellular crude enzyme (culture fluid) of Microbacterium sp. YWIB produced significantly more ethanol from glucose than YWOB but did not from starch. Conclusively, C. tropicalis is thought to catabolize starch dependent upon Microbacterium sp. growing in its cytoplasm and furnish stable habitat for the Microbacterium sp.
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Citations
Citations to this article as recorded by- Tripartite interactions comprising yeast-endobacteria systems in the gut of vector mosquitoes
Alessia Cappelli, Claudia Damiani, Aida Capone, Jovana Bozic, Priscilla Mensah, Emanuela Clementi, Roberta Spaccapelo, Guido Favia, Irene Ricci
Frontiers in Microbiology.2023;[Epub] CrossRef - Uncovering the hidden bacterial ghost communities of yeast and experimental evidences demonstrates yeast as thriving hub for bacteria
B. Indu, Tallapragada Keertana, Sahu Ipsita, Uppada Jagadeeshwari, Chintalapati Sasikala, Chintalapati Venkata Ramana
Scientific Reports.2021;[Epub] CrossRef - Wolbachia pipientis grows in Saccharomyces cerevisiae evoking early death of the host and deregulation of mitochondrial metabolism
Cristina Uribe‐Alvarez, Natalia Chiquete‐Félix, Lilia Morales‐García, Arlette Bohórquez‐Hernández, Norma Laura Delgado‐Buenrostro, Luis Vaca, Antonio Peña, Salvador Uribe‐Carvajal
MicrobiologyOpen.2019;[Epub] CrossRef - Managing plant-environment-symbiont interactions to promote plant performance under low temperature stress
Omid Askari-Khorasgani, Harlene Hatterman-Valenti, Francisco Borja Flores, Mohammad Pessarakli
Journal of Plant Nutrition.2019; 42(16): 2010. CrossRef
- Tripartite interactions comprising yeast-endobacteria systems in the gut of vector mosquitoes
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