MSK
Search
- Page Path
- HOME > Search
Journal Article
- Characterization of MocR, a GntR-like transcriptional regulator, in Bradyrhizobium japonicum: its impact on motility, biofilm formation, and soybean nodulation
- May Nyan Taw , Hae-In Lee , Sang-Ho Lee , Woo-Suk Chang
- J. Microbiol. 2015;53(8):518-525. Published online July 31, 2015
- DOI: https://doi.org/10.1007/s12275-015-5313-z
- 45 View
- 0 Download
- 10 Crossref
-
Abstract
- Bradyrhizobium japonicum is a Gram-negative soil bacterium that can fix nitrogen into ammonia by developing a symbiotic relationship with the soybean plant. MocR proteins make up a subfamily of GntR superfamily, one of the most widely distributed and prolific groups of the helix-turn-helix transcription factors. In this study, we constructed a mutant strain for mocR (blr6977) to investigate its role in cellular processes and symbiosis in B. japonicum. Although growth rate and morphology of the mutant were indistinguishable from those of the wild type, the mutant showed significant differences in motility and attachment (i.e., biofilm formation) from the wild type. The mutant displayed a decrease in biofilm formation, but was more motile than the wild type. The inactivation of mocR did not affect the number of nodules on soybean roots, but caused delayed nodulation. Delayed nodulation intrigued us to study competitiveness of the mutant infecting soybeans. The mutant was less competitive than the wild type, indicating that delayed nodulation might be due to competitiveness. Gene expressions of other MocR subfamily members were also compared between the wild type and mutant strains. None of the mocR-like genes examined in this study were differentially expressed between both strains.
-
Citations
Citations to this article as recorded by
- Antibiofilm efficacies and mechanism of perillaldehyde against Shewanella putrefaciens
Wenxiu Zhu, Yuanhang Cheng, Yankun Zhang, Mingxin Li, Yue Teng, Yunqi Gu, Haisong Wang, Xiaodong Xia
Food Microbiology.2025; 128: 104699. CrossRef - Prevalence and virulence of Vibrio parahaemolyticus isolated from clinical and environmental samples in Huzhou, China
Peng Zhang, Xiaofang Wu, Lei Ji, Wei Yan, Liping Chen, Zhonghao Lu, Deshun Xu, Yunfeng Zha, Dafang Xu, Fenfen Dong
BMC Genomics.2024;[Epub] CrossRef - The MocR family transcriptional regulator DnfR has multiple binding sites and regulates Dirammox gene transcription in Alcaligenes faecalis JQ135
Si‐Qiong Xu, Xiao Wang, Lu Xu, Ke‐Xin Wang, Yin‐Hu Jiang, Fu‐Yin Zhang, Qing Hong, Jian He, Shuang‐Jiang Liu, Ji‐Guo Qiu
Environmental Microbiology.2023; 25(3): 675. CrossRef - The GntR-like transcriptional regulator HutC involved in motility, biofilm-forming ability, and virulence in Vibrio parahaemolyticus
Yangyang Li, Weidong Sun, Quan Wang, Ying Yu, Ying Wan, Kai Zhou, Rong Guo, Xiangan Han, Zhaoguo Chen, Weihuan Fang, Wei Jiang
Microbial Pathogenesis.2022; 167: 105546. CrossRef - Biological and transcriptional studies reveal VmeL is involved in motility, biofilm formation and virulence in Vibrio parahaemolyticus
Peng-xuan Liu, Xiao-yun Zhang, Quan Wang, Yang-yang Li, Wei-dong Sun, Yu Qi, Kai Zhou, Xian-gan Han, Zhao-guo Chen, Wei-huan Fang, Wei Jiang
Frontiers in Microbiology.2022;[Epub] CrossRef - PrbP modulates biofilm formation in Liberibacter crescens
Lei Pan, Christopher L. Gardner, Reagan Beliakoff, Danilo da Silva, Ran Zuo, Fernando A. Pagliai, Kaylie A. Padgett‐Pagliai, Marcelo L. Merli, Erol Bahadiroglu, Claudio F. Gonzalez, Graciela L. Lorca
Environmental Microbiology.2021; 23(11): 7121. CrossRef - The glucose uptake systems in Corynebacterium glutamicum: a review
Haozhe Ruan, Haibo Yu, Jianzhong Xu
World Journal of Microbiology and Biotechnology.2020;[Epub] CrossRef - Comparative Genomics of Pseudomonas sp. Strain SI-3 Associated With Macroalga Ulva prolifera, the Causative Species for Green Tide in the Yellow Sea
Huihui Fu, Peng Jiang, Jin Zhao, Chunhui Wu
Frontiers in Microbiology.2018;[Epub] CrossRef - The MocR‐like transcription factors: pyridoxal 5′‐phosphate‐dependent regulators of bacterial metabolism
Angela Tramonti, Caterina Nardella, Martino L. di Salvo, Stefano Pascarella, Roberto Contestabile
The FEBS Journal.2018; 285(21): 3925. CrossRef - Negative Regulation of Ectoine Uptake and Catabolism in Sinorhizobium meliloti: Characterization of the EhuR Gene
Qinli Yu, Hanlin Cai, Yanfeng Zhang, Yongzhi He, Lincai Chen, Justin Merritt, Shan Zhang, Zhiyang Dong, Anke Becker
Journal of Bacteriology.2017;[Epub] CrossRef
- Antibiofilm efficacies and mechanism of perillaldehyde against Shewanella putrefaciens
First
Prev
TOP
